CN105853768B - Traditional Chinese medicine formula for treating rheumatoid arthritis and preparation method and application thereof - Google Patents
Traditional Chinese medicine formula for treating rheumatoid arthritis and preparation method and application thereof Download PDFInfo
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Abstract
The invention provides a traditional Chinese medicine formula for treating rheumatoid arthritis, which comprises, by mass, 20-40g of Chinese angelica, 20-40g of white paeony root, 20-40g of medicinal cyathula root, 15-30g of tall gastrodia tuber, 10-20g of frankincense, 10-20g of myrrh, 10-20g of dahurian angelica root, 5-10g of giant typhonium rhizome and 2-5g of scorpion.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine formula for treating rheumatoid arthritis and a preparation method thereof.
Background
Rheumatoid arthritis is a chronic systemic autoimmune disease mainly caused by joint diseases, and the pathological features of the rheumatoid arthritis are chronic inflammation of joint synovium, cell infiltration, pannus formation and invasion of cartilage and bone tissues, which cause damage of joint structures, and the rheumatoid arthritis is still one of major diseases threatening human health and life at present. At present, the clinical western medicines mostly adopt nonspecific symptomatic treatment, including glucocorticoid and nonsteroidal anti-inflammatory drug, and drugs for improving the disease condition and resisting rheumatism, and although the symptomatic treatment drugs can improve the symptoms of arthritis of patients, the modern medicine has no specific treatment for the middle and late stages of rheumatoid arthritis. The history of the traditional Chinese medicine for treating rheumatoid arthritis is long, a unique theoretical system is gradually formed, and the traditional Chinese medicine has detailed description on the expression of the arthralgia syndrome and records the application of the prescription and the medicine in the aspect of dialectical treatment of the arthralgia syndrome. In recent years, with the wide and deep development of traditional Chinese medicine research on the treatment of rheumatoid arthritis, the traditional Chinese medicine and western medicine combined treatment or the combined administration of oral administration and external application of the traditional Chinese medicine are adopted clinically, so that the good curative effect is obtained, and meanwhile, the application of the traditional Chinese medicine has the advantages of stable curative effect, safety, small adverse reaction and the like, and becomes one of indispensable effective methods for treating the rheumatoid arthritis.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides a traditional Chinese medicine formula for treating rheumatoid arthritis, and therefore, the invention also provides a preparation method and application thereof.
The invention aims to provide a traditional Chinese medicine formula for treating rheumatoid arthritis, which comprises the following raw materials in parts by mass: 20-40g of angelica, 20-40g of white peony root, 20-40g of medicinal cyathula root, 15-30g of gastrodia elata, 10-20g of frankincense, 10-20g of myrrh, 10-20g of angelica dahurica, 5-10g of rhizoma typhonii and 2-5g of scorpion.
The second purpose of the invention is to provide a preparation method of the traditional Chinese medicine formula, which comprises the following steps:
(1) weighing the raw material medicines according to the formula amount;
(2) pulverizing Scorpio into fine powder;
(3) mixing radix Angelicae sinensis, Olibanum, Myrrha, radix Angelicae Dahuricae, and rhizoma Typhonii, adding water, extracting volatile oil by steam distillation, and collecting volatile oil A; filtering the mother liquor, collecting the residue, concentrating the filtrate, precipitating with ethanol, and filtering to obtain filtrate B;
(4) mixing the residue obtained in step (3) with radix Paeoniae alba, radix Cyathulae and rhizoma Gastrodiae, extracting with ethanol, and filtering to obtain filtrate C;
(5) mixing the extractive solution B and the extractive solution C, concentrating, recovering ethanol, and adding Scorpio powder during concentration to obtain soft extract D;
(6) clathrating the volatile oil with β -cyclodextrin to obtain clathrate E;
(7) mixing the soft extract D, β -cyclodextrin clathrate E, and sodium carboxymethylcellulose, granulating, drying, and making into capsule.
Preferably, in the step (2), the scorpion is ground into fine powder and then sieved by a sieve of 80-120 meshes.
Preferably, in the step (3), after the angelica, the frankincense, the myrrh, the angelica dahurica and the rhizoma typhonii are mixed, water is added according to 10-15 times of the weight of the mixture, the volatile oil is extracted for 6-8 hours by a steam distillation method, and the volatile oil A is collected;
preferably, in the step (3), the filtrate is concentrated until the mass ratio of the medicinal materials to the liquid medicine is 1: 1;
preferably, in the step (3), the alcohol precipitation is performed by using 2 times of 75% -95% ethanol by weight, the alcohol precipitation temperature is 40-60 ℃, and the standing is performed for 16-24 hours.
Preferably, in the step (4), the alcohol extraction is performed by mixing the dregs of decoction obtained after the volatile oil is extracted from the medicinal materials with the white paeony root, the medicinal cyathula root and the tall gastrodia tuber, adding ethanol with the weight being 10-15 times of that of the medicinal materials for alcohol extraction twice, wherein the ethanol concentration is 55-70% in the first time, the ethanol concentration is 75-90% in the second time, and the extraction time is 1.0-1.5 hours, and combining the alcohol extraction solutions.
Preferably, in the step (5), the ethanol is concentrated and recovered at the temperature of 80-90 ℃;
preferably, the thick paste D has a specific gravity of 1.25 to 1.30(55 to 65 ℃).
Preferably, in the step (6), the inclusion is carried out by the specific parameters of β -cyclodextrin and water in a weight ratio of 1: 2-5 and the weight ratio of the volatile oil A to β -cyclodextrin in a weight ratio of 1: 2-8, stirring for 2 hours during inclusion, carrying out inclusion at 50 ℃, filtering out the inclusion complex, and drying at 40-60 ℃ to obtain the inclusion complex E.
The third purpose of the invention is to provide the application of the traditional Chinese medicine formula in preparing the medicine for treating rheumatoid arthritis, rheumatic arthritis, ankylosing spondylitis or rheumatic cold arthralgia.
The fourth purpose of the invention is to provide a medicine, and the effective components of the medicine are the traditional Chinese medicine formula.
Preferably, the medicament further comprises medically acceptable auxiliary materials.
Preferably, the dosage form of the medicine is capsules or granules.
Rheumatic diseases are diseases which are different in pain but involve joints and surrounding soft tissues including muscles, ligaments, bursa of slipping and fascia, and are caused by joint pain, stiffness in the morning, swelling and tenderness, limited activity and a chronic process of alternating attack and relief, and some patients can suffer from whole body muscle weakness, pain, joint disability and viscera function weakness. The diseases are in the scope of arthralgia and flaccidity symptoms of traditional Chinese medicine, and are caused by liver and kidney deficiency, external pathogenic factors such as wind, cold and dampness attacking human body, blocking channels and collaterals and unsmooth circulation of qi and blood. The book of emperor's internal channel, questions and Bi Lun says that the combination of three qi of wind-cold-dampness is also arthralgia and that the qi must be deficient. Based on this theory, the recipe uses the actions of dispelling wind and removing dampness, nourishing blood and dispelling cold, and strengthening healthy qi to eliminate pathogenic factors, so called "self-extinguishing of blood-moving wind", "internal storage of healthy qi and no drying of pathogenic factors". The medicines used in the prescription have the effects of expelling wind and removing cold, and the traditional Chinese medicine theory considers that the arthralgia syndrome is 'wind-cold-dampness, three qi mixed and even arthralgia'. Wind is the first to treat all kinds of diseases, so the main treatment for arthralgia syndrome is wind. The angelica and the rhizoma gastrodiae in the formula enrich the blood and promote blood circulation, and remove blood stasis and relieve pain; radix Cyathulae has effects of removing blood stasis, dredging channels, dispelling pathogenic wind, removing dampness, and dredging joint; radix Paeoniae alba can nourish blood and soften liver, and relieve epigastric distention and pain; the angelica dahurica dispels wind and dampness, and reduces swelling and relieves pain; it is combined with Bai Fu Zi and quan Xie to strengthen the actions of removing toxicity, dissipating nodulation and alleviating pain; ru Xiang and Mo Yao are the essential herbs for dredging the zang-fu organs and circulating meridians and collaterals when used together. The whole formula has the effects of expelling wind and clearing away cold, and removing dampness and removing arthralgia.
The modern pharmacological research of the efficacy is summarized as follows:
chinese angelica: has the functions of enriching the blood; promoting blood circulation; regulating menstruation and relieving pain; moistening dryness and smoothing intestine. It can be used for treating blood deficiency syndrome, menoxenia, amenorrhea, dysmenorrhea, abdominal mass, metrorrhagia, asthenia cold, abdominal pain, flaccidity arthralgia, numbness of skin, intestinal dryness, constipation, dysentery, carbuncle, skin ulcer, traumatic injury. The main components of the compound are organic acids and volatile oil, and the modern pharmacological research shows that the compound has the effects of resisting thrombus, improving blood circulation, improving coronary circulation, resisting inflammation, relieving pain, protecting liver, benefiting gallbladder, enriching blood and the like.
White peony root: has effects in nourishing blood, softening liver, relieving pain, astringing yin, and arresting sweating. It can be used for treating chest and abdomen pain, hypochondriac pain, dysentery, abdominal pain, spontaneous perspiration, night sweat, fever due to yin deficiency, menoxenia, metrorrhagia, leukorrhagia, etc. It is mainly used for headache, arthralgia and numbness of limbs. It is combined with Chuan Xiong to treat headache, and combined with quan Xie and Ru Xiang to treat arthralgia. In addition, for numbness of limbs and paralysis of hands and feet, it is commonly used in clinic with Dang Gui and niu xi. It should contain glycosides. Modern pharmacological research shows that the medicine has the functions of regulating immune system, resisting atherosclerosis, protecting heart, etc.
Radix cyathulae: has effects in dispelling blood stasis, dredging channels, promoting joint movement, and inducing diuresis for treating stranguria. Can be used for treating amenorrhea, abdominal mass, retained afterbirth, arthralgia, flaccidity of feet, spasm of tendons, stranguria with hematuria and blood, and traumatic injury. The composition mainly contains sterone and polysaccharide compounds, and the modern pharmacological research shows that the composition has the functions of regulating the immune system, protecting the cardiovascular and cerebrovascular systems and resisting tumors.
Gastrodia elata: has effects of calming endogenous wind, relieving spasm, suppressing liver yang, dispelling pathogenic wind, and dredging collaterals. Can be used for treating infantile convulsion, epilepsy, convulsion, tetanus, headache, vertigo, limb paralysis, numbness of limbs, and rheumatalgia. The medicine mainly contains phenols, glycosides, polysaccharide compounds and the like, and modern pharmacological research shows that the medicine has the effects of tranquilizing and resisting convulsion, relieving pain, resisting inflammation, improving immunity and protecting the cardiovascular system.
Ru Xiang and Mo Yao can activate blood and resolve stasis, move qi and alleviate pain. But frankincense is pungent and fragrant, and is better at relieving pain mainly by promoting qi and blood circulation; mo Yao is bitter in flavor and good at promoting blood circulation and dissipating blood stasis, so it is good at relieving swelling. Compendium of materia Medica: "dispel blood and relieve swelling, relieve pain and promote tissue regeneration". "Ru Xiang is used for activating blood and Mo Yao for dispersing blood, so it can alleviate pain, resolve swelling and promote granulation, so they are used together. "
Radix angelicae: has effects in expelling pathogenic wind, relieving exterior syndrome, expelling cold, relieving pain, eliminating dampness, dredging orifice, and relieving swelling and pus. It can be used for treating headache, toothache, nasosinusitis, hemorrhoid, leucorrhea with red and white discharge, carbuncle, skin ulcer, and skin pruritus. The traditional Chinese medicine composition mainly contains components such as volatile oil, and modern pharmacological research shows that the traditional Chinese medicine composition has the effects of relieving fever, easing pain, resisting inflammation and the like and can improve local blood circulation.
Giant typhonium rhizome: has the effects of dispelling pathogenic wind and phlegm, arresting convulsion, removing toxic substance, resolving hard mass and relieving pain. Can be used for treating apoplexy with phlegm stagnation, facial distortion, phlegm syncope, headache, migraine, etc. The compound mainly contains components such as fatty acid, steroid compounds, volatile oil and the like, and the modern pharmacological research shows that the compound has the effects of calming, resisting inflammation, relieving pain, resisting convulsion, resisting tumors and the like.
Scorpion: has effects in calming endogenous wind, relieving spasm, removing toxic materials, resolving hard mass, dredging meridians, and relieving pain. Can be used for treating syndrome of internal stirring of liver wind, carbuncle, swelling, sore and ulcer, scrofula, goiter, intractable rheumatism, intractable headache, facial distortion, etc. The traditional Chinese medicine mainly contains components such as protein, amino acid and the like, and modern pharmacological research shows that the traditional Chinese medicine has the effects of analgesia, anticoagulation, anti-tumor, anti-epilepsy and the like.
Compared with the prior art, the invention has the following advantages:
1. in the prior art, the original formula is a capsule formulation, but the preparation process is simple and crude, and scientific extraction and preparation processes are lacked, the content of the capsule is that the medicinal materials are directly filled into the capsule after being crushed, the crude medicines are directly taken without extraction, the content of the effective components of the medicine is low, and the dosage is large. The invention improves the existing medicine preparation method, classifies the properties of various active ingredients contained in the medicinal materials of the prescription, selects different extraction methods to respectively extract, and changes the solubility of a solvent by changing the concentration of ethanol to remove impurities such as tannin, resin, fat-soluble pigment, protein and the like, thereby achieving the purpose of coarse extraction and fine extraction and improving the content of the active ingredients of the medicine contained in unit weight. The effective content of the medicine taken every day of the medicine prepared by the process is 15-18 times of that of the medicine processed by the prior art, so that the curative effect of clinical medicine application is improved by 10-13%.
2. Because the angelica, the angelica dahurica, the frankincense, the myrrh and the like in the formula all contain volatile substances, the main effective components of the medicinal materials are volatile oil and fat-soluble substances, the volatile oil is easy to volatilize when the temperature is higher than 60 ℃, the water decoction temperature reaches 100 ℃, the volatile components are greatly dissipated, and the lipid components can not be completely extracted in water, so that the original effect of the formula is lost, and the two substances are easy to dissolve in a low-polarity organic solvent and have low solubility in water, therefore, the extraction process firstly adopts a steam distillation method to extract the volatile components, and then mother liquor is reserved, so that the volatile oil and the fat-soluble substances are effectively obtained, and the water-soluble substances which are not completely extracted during extraction are reserved.
3. The invention adopts β -cyclodextrin inclusion technology to carry out inclusion on the volatile oil, the medicine is included in the molecular capsule, the release can be controlled, the irritation and the side effect are reduced, the volatilization is reduced, the liquid medicine is powdered, and β -cyclodextrin is carbohydrate which can be absorbed and utilized by human body without accumulation.
Detailed Description
The following examples are given to facilitate a better understanding of the invention, but do not limit the invention. The experimental procedures in the following examples are conventional unless otherwise specified. The test materials used in the following examples are commercially available unless otherwise specified.
The traditional Chinese medicine for treating rheumatoid arthritis comprises the following components:
20-40g of angelica, 20-40g of white peony root, 20-40g of medicinal cyathula root, 15-30g of gastrodia elata, 10-20g of frankincense, 10-20g of myrrh, 10-20g of angelica dahurica, 5-10g of rhizoma typhonii and 2-5g of scorpion.
The preparation method of the traditional Chinese medicine formula for treating rheumatoid arthritis comprises the following steps:
(1) weighing the following raw medicines in parts by weight: 20-40g of angelica, 20-40g of white peony root, 20-40g of medicinal cyathula root, 15-30g of gastrodia elata, 10-20g of frankincense, 10-20g of myrrh, 10-20g of angelica dahurica, 5-10g of rhizoma typhonii and 2-5g of scorpion;
(2) pulverizing Scorpio into fine powder, and sieving with 80-120 mesh sieve;
(3) mixing the angelica, the frankincense, the myrrh, the angelica and the rhizoma typhonii, adding water according to 10-15 times of the weight of the mixture, extracting volatile oil for 6-8 hours by a steam distillation method, collecting the volatile oil A, and storing in a sealed manner; filtering the mother liquor for extracting the volatile oil, reserving the dregs, and concentrating the filtrate to the following medicinal materials: when the liquid medicine is =1:1(w/w), carrying out alcohol precipitation by using 75% -95% ethanol with 2 times of weight, wherein the alcohol precipitation temperature is 40-60 ℃, standing for 16-24 hours, and filtering to obtain filtrate B;
(4) mixing the dregs of decoction obtained after the volatile oil is extracted from the medicinal materials with the white paeony root, the medicinal cyathula root and the tall gastrodia tuber, adding ethanol with the weight being 10-15 times of that of the medicinal materials for ethanol extraction twice, wherein the ethanol concentration of the ethanol is 55-70% for the first time, the ethanol concentration of the ethanol is 75-90% for the second time, the extraction time is 1.0-1.5 hours, combining the ethanol extract, and filtering to obtain a filtrate C;
(5) mixing the extractive solutions B and C, concentrating at 80-90 deg.C for recovering ethanol, and adding Scorpio powder during concentration process until soft extract D with specific gravity of 1.25-1.30(55-65 deg.C) is obtained;
(6) coating the volatile oil A with β -cyclodextrin, wherein the weight ratio of β -cyclodextrin to water is =1: 2-5, the weight ratio of volatile oil A to β -cyclodextrin is =1: 2-8, stirring for 2 hours, the coating temperature is 50 ℃, filtering out the inclusion complex, and drying at 40-60 ℃ to obtain an inclusion complex E;
(7) mixing the thick paste D, β -cyclodextrin inclusion compound E and sodium carboxymethyl cellulose, wherein the addition amount of the sodium carboxymethyl cellulose is 2-6% of the total weight of the thick paste D and β -cyclodextrin inclusion compound E, uniformly mixing, granulating, drying at 50-70 ℃, and encapsulating with 0.45 g/granule.
The functional indications are as follows:
dispelling cold and eliminating dampness, relaxing muscles and tendons, promoting blood circulation, invigorating qi and blood, and relieving swelling and pain. Can be used for treating rheumatic arthritis and rheumatoid arthritis.
The usage and dosage are as follows:
capsule, 0.45 g/granule. Orally administered 4 granules at a time, 3 times a day.
The clinical use condition of the traditional Chinese medicine formula of the invention
(1) The selection of cases comprises selecting 125 cases of medical records, 44 cases of men and 81 cases of women, age 56-76 years, and course of disease 4-240 days, wherein 39 cases of rheumatoid arthritis, 21 cases of rheumatoid arthritis, 11 cases of ankylosing spondylitis, and 49 cases of rheumatic-cold arthralgia.
(2) The diagnosis standard refers to the rheumatoid arthritis diagnosis standard revised by the American college of rheumatology in 1987 in all cases, which comprises ① morning stiffness lasting at least 1 hour every day, the course of disease lasting at least 6 weeks, ② 3 or more joint swelling lasting at least 6 weeks, ③ hand joint, palm joint or proximal interphalangeal joint swelling lasting at least 6 weeks, ④ joint symmetric swelling lasting at least 6 weeks, ⑤ rheumatoid nodules, ⑥ rheumatoid factor positive, ⑦ X-ray photograph showing hand/wrist joint osteoporosis or obvious decalcification signs, 4 of the 7 items can be confirmed, and the Chinese medicine syndrome differentiation standard refers to the rheumatoid arthritis inclusion and exclusion standard of the cold-dampness arthralgia syndrome of the Chinese medicine clinical research guide principle (trial) formulated by the Ministry of health of the people's republic of China in 2002.
(3) The treatment method comprises the following steps: the traditional Chinese medicine composition is orally taken 4 granules at a time, 3 times a day and 1 month as a treatment course.
(4) The evaluation standard of curative effect is as follows: the method is formulated according to the Chinese medicine administration 'Standard of curative Effect for diagnosing Chinese medical diseases'. And (3) healing: symptoms and physical signs disappear completely, joint function activity is normal, and laboratory examination is normal; the effect is shown: the symptoms and signs are mostly disappeared or reduced, the joint function activity is basically normal, and the laboratory checks that the joint function activity is normal or basically returns to normal; the method has the following advantages: the symptoms and physical signs are reduced, the joint function activity is improved, and the experimental indexes are improved; and (4) invalidation: symptoms, physical signs and joint function activities are not obviously improved, and experimental examination is unchanged.
(5) The treatment results are as follows: and (3) healing: 13 cases (10.4%), significant effect: 43 cases (34.4%), effective: 53 (42.4%), null: 16 cases (12.8%), total effective rate: 109 cases (87.2%).
Example 1
The traditional Chinese medicine for treating rheumatoid arthritis comprises the following components:
30g of angelica, 30g of white peony root, 30g of medicinal cyathula root, 20g of gastrodia elata, 15g of frankincense, 15g of myrrh, 15g of angelica dahurica, 5g of rhizoma typhonii and 2g of scorpion.
The preparation method of the traditional Chinese medicine formula for treating rheumatoid arthritis comprises the following steps:
(1) weighing the following raw medicines in parts by weight: 30g of angelica, 30g of white peony root, 30g of medicinal cyathula root, 20g of gastrodia elata, 15g of frankincense, 15g of myrrh, 15g of angelica dahurica, 5g of rhizoma typhonii and 2g of scorpion;
(2) pulverizing Scorpio into fine powder, and sieving with 100 mesh sieve;
(3) mixing radix Angelicae sinensis, Olibanum, Myrrha, radix Angelicae Dahuricae, and rhizoma Typhonii, adding water 12 times of the mixture, extracting volatile oil by steam distillation for 7 hr, collecting volatile oil A, and sealing; filtering the mother liquor for extracting the volatile oil, reserving the dregs, and concentrating the filtrate to the following medicinal materials: precipitating with 2 times of 85% ethanol at 50 deg.C for 20 hr when the medicinal liquid is =1:1(w/w), and filtering to obtain filtrate B;
(4) mixing the residues after extracting volatile oil from the medicinal materials with radix paeoniae alba, radix cyathulae and rhizoma gastrodiae, adding ethanol with the weight being 12 times of that of the medicinal materials for ethanol extraction, extracting twice, wherein the ethanol concentration is 60% for the first time and 85% for the second time, extracting for 1.2 hours, combining ethanol extract, filtering, and taking the filtrate as C;
(5) mixing the extractive solution B and the extractive solution C, concentrating at 85 deg.C for recovering ethanol, and adding Scorpio powder during concentration process until soft extract D with a weight of 1.25 (60 deg.C) is obtained;
(6) coating the volatile oil A with β -cyclodextrin, wherein the weight ratio of β -cyclodextrin to water is =1: 4, and the weight ratio of volatile oil A to β -cyclodextrin is =1: 6, stirring for 2 hours, at the coating temperature of 50 ℃, filtering out the inclusion complex, and drying at 50 ℃ to obtain an inclusion complex E;
(7) mixing the above soft extract D, β -cyclodextrin clathrate E, and sodium carboxymethylcellulose 4% of the total weight of soft extract D and β -cyclodextrin clathrate E, granulating, drying at 60 deg.C, and making into capsule at a dose of 0.45 g/granule.
Example 2
The traditional Chinese medicine for treating rheumatoid arthritis comprises the following components:
20g of angelica, 20g of white peony root, 20g of medicinal cyathula root, 15g of gastrodia elata, 10g of frankincense, 10g of myrrh, 10g of angelica dahurica, 5g of rhizoma typhonii and 2g of scorpion.
The preparation method of the traditional Chinese medicine formula for treating rheumatoid arthritis comprises the following steps:
(1) weighing the following raw medicines in parts by weight: 20g of angelica, 20g of white peony root, 20g of medicinal cyathula root, 15g of gastrodia elata, 10g of frankincense, 10g of myrrh, 10g of angelica dahurica, 5g of rhizoma typhonii and 2g of scorpion;
(2) pulverizing Scorpio into fine powder, and sieving with 80 mesh sieve;
(3) mixing radix Angelicae sinensis, Olibanum, Myrrha, radix Angelicae Dahuricae, and rhizoma Typhonii, adding water 10 times of the mixture, extracting volatile oil by steam distillation for 6 hr, collecting volatile oil A, and sealing; filtering the mother liquor for extracting the volatile oil, reserving the dregs, and concentrating the filtrate to the following medicinal materials: precipitating with 2 times of 75% ethanol at 60 deg.C for 24 hr when the liquid medicine is =1:1(w/w), and filtering to obtain filtrate B;
(4) mixing the residues after the volatile oil is extracted from the medicinal materials with the white paeony root, the medicinal cyathula root and the tall gastrodia tuber, adding ethanol with the weight being 10 times that of the medicinal materials for ethanol extraction, extracting for two times, wherein the ethanol concentration is 55 percent for the first time and 90 percent for the second time for 1.0 hour, combining ethanol extract, and filtering to obtain filtrate C;
(5) mixing the extractive solution B and the extractive solution C, concentrating at 80 deg.C for recovering ethanol, and adding Scorpio powder during concentration process until soft extract D with specific gravity of 1.30(65 deg.C) is obtained;
(6) coating the volatile oil A with β -cyclodextrin, wherein the weight ratio of β -cyclodextrin to water is =1: 5, the weight ratio of volatile oil A to β -cyclodextrin is =1: 8, stirring for 2 hours, at the coating temperature of 50 ℃, filtering out the inclusion complex, and drying at the temperature of 40 ℃ to obtain an inclusion complex E;
(7) mixing the above soft extract D, β -cyclodextrin inclusion compound E, and sodium carboxymethylcellulose 6 wt% of the soft extract D and β -cyclodextrin inclusion compound E, granulating, drying at 70 deg.C, and making into capsule at a dose of 0.45 g/granule.
Example 3
The traditional Chinese medicine for treating rheumatoid arthritis comprises the following components:
40g of angelica, 40g of white peony root, 40g of medicinal cyathula root, 30g of gastrodia elata, 20g of frankincense, 20g of myrrh, 20g of angelica dahurica, 10g of rhizoma typhonii and 5g of scorpion.
The preparation method of the traditional Chinese medicine formula for treating rheumatoid arthritis comprises the following steps:
(1) weighing the following raw medicines in parts by weight: 40g of angelica, 40g of white peony root, 40g of medicinal cyathula root, 30g of gastrodia elata, 20g of frankincense, 20g of myrrh, 20g of angelica dahurica, 10g of rhizoma typhonii and 5g of scorpion;
(2) pulverizing Scorpio into fine powder, and sieving with 120 mesh sieve;
(3) mixing radix Angelicae sinensis, Olibanum, Myrrha, radix Angelicae Dahuricae, and rhizoma Typhonii, adding water 15 times of the mixture, extracting volatile oil by steam distillation for 8 hr, collecting volatile oil A, and sealing; filtering the mother liquor for extracting the volatile oil, reserving the dregs, and concentrating the filtrate to the following medicinal materials: precipitating with 2 times of 95% ethanol at 40 deg.C for 16 hr when the liquid medicine is =1:1(w/w), and filtering to obtain filtrate B;
(4) mixing the residues after extracting volatile oil from the medicinal materials with radix paeoniae alba, radix cyathulae and rhizoma gastrodiae, adding ethanol 15 times of the weight of the medicinal materials for ethanol extraction, extracting twice, wherein the ethanol concentration is 70% for the first time, and 75% for the second time, extracting for 1.5 hours, combining ethanol extract, filtering, and taking the filtrate as C;
(5) mixing the extractive solution B and the extractive solution C, concentrating at 90 deg.C for recovering ethanol, and adding Scorpio powder during concentration process until soft extract D with a weight of 1.25 (60 deg.C) is obtained;
(6) coating the volatile oil A with β -cyclodextrin, wherein the weight ratio of β -cyclodextrin to water is =1: 2, the weight ratio of volatile oil A to β -cyclodextrin is =1: 2, stirring for 2 hours, at the coating temperature of 50 ℃, filtering out the inclusion complex, and drying at 60 ℃ to obtain an inclusion complex E;
(7) mixing the above soft extract D, β -cyclodextrin inclusion compound E, and sodium carboxymethylcellulose (2 wt% of the soft extract D and β -cyclodextrin inclusion compound E), granulating, drying at 50 deg.C, and making into capsule (0.45 g/granule).
Example 4
The traditional Chinese medicine for treating rheumatoid arthritis comprises the following components:
25g of angelica, 25g of white peony root, 25g of medicinal cyathula root, 20g of gastrodia elata, 15g of frankincense, 15g of myrrh, 15g of angelica dahurica, 5g of rhizoma typhonii and 2g of scorpion.
The preparation method of the traditional Chinese medicine formula for treating rheumatoid arthritis comprises the following steps:
(1) weighing the following raw medicines in parts by weight: 25g of angelica, 25g of white peony root, 25g of medicinal cyathula root, 20g of gastrodia elata, 15g of frankincense, 15g of myrrh, 15g of angelica dahurica, 5g of rhizoma typhonii and 2g of scorpion;
(2) pulverizing Scorpio into fine powder, and sieving with 100 mesh sieve;
(3) mixing radix Angelicae sinensis, Olibanum, Myrrha, radix Angelicae Dahuricae, and rhizoma Typhonii, adding water 13 times of the mixture, extracting volatile oil by steam distillation for 7 hr, collecting volatile oil A, and sealing; filtering the mother liquor for extracting the volatile oil, reserving the dregs, and concentrating the filtrate to the following medicinal materials: precipitating with 2 times of 80% ethanol at 45 deg.C for 18 hr when the liquid medicine is =1:1(w/w), and filtering to obtain filtrate B;
(4) mixing the residues after extracting volatile oil from the medicinal materials with radix paeoniae alba, radix cyathulae and rhizoma gastrodiae, adding ethanol 13 times of the weight of the medicinal materials for ethanol extraction twice, extracting for 1.0 hour for the first time at the ethanol concentration of 60% and the second time at the ethanol concentration of 85%, combining ethanol extract, filtering, and taking the filtrate as C;
(5) mixing the extractive solution B and the extractive solution C, concentrating at 80 deg.C for recovering ethanol, and adding Scorpio powder during concentration process until soft extract D with specific gravity of 1.25 (65 deg.C) is obtained;
(6) coating the volatile oil A with β -cyclodextrin, wherein the weight ratio of β -cyclodextrin to water is =1: 5, the weight ratio of volatile oil A to β -cyclodextrin is =1: 8, stirring for 2 hours, at the coating temperature of 50 ℃, filtering out the inclusion complex, and drying at 55 ℃ to obtain an inclusion complex E;
(7) mixing the above soft extract D, β -cyclodextrin inclusion compound E, and sodium carboxymethylcellulose 5 wt% of the soft extract D and β -cyclodextrin inclusion compound E, granulating, drying at 55 deg.C, and making into capsule at a dose of 0.45 g/granule.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (14)
1. A traditional Chinese medicine for treating rheumatoid arthritis is characterized in that: the traditional Chinese medicine is prepared from the following raw material medicines by mass: 20-40g of angelica sinensis, 20-40g of radix paeoniae alba, 20-40g of radix cyathulae, 15-30g of rhizoma gastrodiae, 10-20g of frankincense, 10-20g of myrrh, 10-20g of radix angelicae, 5-10g of rhizoma typhonii and 2-5g of scorpion, wherein the preparation method of the traditional Chinese medicine comprises the following steps:
(1) weighing the raw material medicines according to the formula amount;
(2) pulverizing Scorpio into fine powder;
(3) mixing radix Angelicae sinensis, Olibanum, Myrrha, radix Angelicae Dahuricae, and rhizoma Typhonii, adding water, extracting volatile oil by steam distillation, and collecting volatile oil A; filtering the mother liquor, collecting the residue, concentrating the filtrate, precipitating with ethanol, and filtering to obtain filtrate B;
(4) mixing the residue obtained in step (3) with radix Paeoniae alba, radix Cyathulae and rhizoma Gastrodiae, extracting with ethanol, and filtering to obtain filtrate C;
(5) mixing the extractive solution B and the extractive solution C, concentrating, recovering ethanol, and adding Scorpio powder during concentration to obtain soft extract D;
(6) clathrating the volatile oil with β -cyclodextrin to obtain clathrate E;
(7) mixing the soft extract D, β -cyclodextrin clathrate E, and sodium carboxymethylcellulose, granulating, drying, and making into capsule.
2. The preparation method of the traditional Chinese medicine of claim 1, which is characterized by comprising the following steps:
(1) weighing the raw material medicines according to the formula amount;
(2) pulverizing Scorpio into fine powder;
(3) mixing radix Angelicae sinensis, Olibanum, Myrrha, radix Angelicae Dahuricae, and rhizoma Typhonii, adding water, extracting volatile oil by steam distillation, and collecting volatile oil A; filtering the mother liquor, collecting the residue, concentrating the filtrate, precipitating with ethanol, and filtering to obtain filtrate B;
(4) mixing the residue obtained in step (3) with radix Paeoniae alba, radix Cyathulae and rhizoma Gastrodiae, extracting with ethanol, and filtering to obtain filtrate C;
(5) mixing the extractive solution B and the extractive solution C, concentrating, recovering ethanol, and adding Scorpio powder during concentration to obtain soft extract D;
(6) clathrating the volatile oil with β -cyclodextrin to obtain clathrate E;
(7) mixing the soft extract D, β -cyclodextrin clathrate E, and sodium carboxymethylcellulose, granulating, drying, and making into capsule.
3. The method of claim 2, wherein: in the step (2), the scorpion is crushed into fine powder and sieved by a sieve of 80-120 meshes.
4. The method of claim 2, wherein: in the step (3), after the angelica, the frankincense, the myrrh, the angelica dahurica and the rhizoma typhonii are mixed, water is added according to 10-15 times of the weight of the mixture, the volatile oil is extracted for 6-8 hours by a steam distillation method, and the volatile oil A is collected.
5. The method of claim 2, wherein: in the step (3), the filtrate is concentrated until the mass ratio of the medicinal materials to the liquid medicine is 1: 1.
6. The method of claim 2, wherein: in the step (3), the alcohol precipitation is performed by using 2 times of 75-95% ethanol by weight, the alcohol precipitation temperature is 40-60 ℃, and the standing is performed for 16-24 hours.
7. The method of claim 2, wherein: in the step (4), the alcohol extraction is to mix the dregs of a decoction obtained after the volatile oil is extracted from the medicinal materials with the white paeony root, the medicinal cyathula root and the tall gastrodia tuber, add 10-15 times of ethanol for alcohol extraction twice, extract the medicinal materials for the first time with the ethanol concentration of 55-70% and extract the medicinal materials for the second time with the ethanol concentration of 75-90% for 1.0-1.5 hours, and combine the alcohol extract.
8. The method of claim 2, wherein: in the step (5), the ethanol is concentrated and recovered at the temperature of 80-90 ℃.
9. The method of claim 2, wherein: the specific gravity of the thick paste D is 1.25-1.30, and the temperature range of the thick paste D is 55-65 ℃.
10. The method as claimed in claim 2, wherein in step (6), the inclusion is carried out by mixing β -cyclodextrin with water at a weight ratio of 1: 2-5, mixing volatile oil A with β -cyclodextrin at a weight ratio of 1: 2-8, stirring for 2 hr, inclusion at 50 deg.C, filtering out inclusion complex, and drying at 40-60 deg.C to obtain inclusion complex E.
11. The use of the Chinese medicament of claim 1 in the preparation of a medicament for the treatment of rheumatoid arthritis, rheumatoid arthritis or rheumatic cold joint pain.
12. A medicament, characterized by: the effective component of the medicine is the traditional Chinese medicine of claim 1.
13. The medicament of claim 12, wherein: the dosage form of the medicine is capsule or granule.
14. The medicament of claim 12, wherein: the medicine also comprises medically acceptable auxiliary materials.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1857541A (en) * | 2006-03-15 | 2006-11-08 | 刘少华 | Plaster for treating rheumatism and osteoarthrosis |
| CN101085304A (en) * | 2007-06-18 | 2007-12-12 | 付先进 | Plaster mainly for curing cervical vertebra and lumbar vertebra disease |
| CN102961610A (en) * | 2012-12-06 | 2013-03-13 | 宁英琪 | Topical paste patch |
| CN104337970A (en) * | 2013-07-30 | 2015-02-11 | 辜朝阳 | External medicinal liquor for treating rheumatoid arthritis |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1857541A (en) * | 2006-03-15 | 2006-11-08 | 刘少华 | Plaster for treating rheumatism and osteoarthrosis |
| CN101085304A (en) * | 2007-06-18 | 2007-12-12 | 付先进 | Plaster mainly for curing cervical vertebra and lumbar vertebra disease |
| CN102961610A (en) * | 2012-12-06 | 2013-03-13 | 宁英琪 | Topical paste patch |
| CN104337970A (en) * | 2013-07-30 | 2015-02-11 | 辜朝阳 | External medicinal liquor for treating rheumatoid arthritis |
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