CN105410365A - Feed additive capable of replacing antibiotics and application of feed additive - Google Patents

Abstract

The invention discloses a feed additive which can replace antibiotics and an application thereof. In terms of weight percentage, the feed additive comprises: 10-20% monoglyceride laurate; 20-40% dilaurin glyceride; monostearic acid Glyceride 30-60%; Carrier 0-40%. The present invention uses glyceryl monolaurate, glyceryl dilaurate and glyceryl monostearate as the main raw materials, and the feed additive prepared according to the appropriate ratio can replace antibiotics in piglet breeding and improve the disease resistance of piglets. Promote intestinal development and intestinal function improvement, significantly increase daily weight gain and feed intake, significantly reduce diarrhea rate and death rate, improve feed conversion rate, promote growth and development, improve breeding economic benefits, reduce antibiotic use, and reduce environmental pollution , which is conducive to promoting the green, safe and healthy development of the pig industry.

Description

A feed additive that can replace antibiotics and its application

technical field

The invention relates to the technical field of feed additives, in particular to a feed additive that can replace antibiotics and its application.

Background technique

In the high-density large-scale breeding mode, in order to make the pigs go out of the market as soon as possible and reduce illness and death, heavy metals, hormones, antibiotic additives and various drugs are often used in excess of the standard in the piglet breeding process, and these substances remain in the piglets. Entering the environment with the excrement from farming seriously affects human health.

With people's attention to food safety and human health, the requirements for meat products have changed, and it is urgent to market green, safe and nutritious products. Therefore, it is necessary to use safe, efficient, and green functional food ingredients to develop feed additives to replace the application of antibiotics in piglet breeding, not only without heavy metals, hormones, and antibiotic residues, but also to improve disease resistance and promote intestinal development. And intestinal function is perfect, increase daily weight gain and feed intake, reduce mortality rate, improve feed conversion rate, promote growth and development, improve economic benefits, and reduce environmental pollution.

Glyceryl monolaurate has broad-spectrum antibacterial activity and specific antiviral ability; glyceryl dilaurate itself does not have antibacterial and antiviral ability, but it can be released in the intestinal tract through the metabolism of intestinal flora and the action of digestive enzymes Lauric acid, which has potential antibacterial and antiviral ability; glyceryl monostearate, which has excellent emulsifying function, is an internationally recognized safe, non-toxic, multi-functional food additive, and is widely used in various foods, Among cosmetics and pharmaceutical products, it is a GRAS (Generally Recognized As Safe) food additive recognized by the FDA in the United States, and it is also a safe additive approved by the Ministry of Health of China for use in various foods and feeds.

At present, the above-mentioned components are mainly used to prepare feed additives with antiseptic and anti-mildew functions, such as:

The invention patent document with the authorized notification number CN1235520C discloses a glyceryl monolaurate microemulsion and its preparation method and application. Agent 10-30%; glyceryl monolaurate 1-20%; water or sodium chloride or organic weak salt 15-40%. This product can be widely used in food and cosmetics to play the role of anti-corrosion, anti-mildew and prolonging the shelf life of products.

The invention patent application document with the application publication number CN101797011A discloses a feed antifungal agent, which contains glycerol monolaurate, sodium dehydroacetate, calcium propionate and a carrier. The antifungal agent for feed is added in a small amount and does not affect the palatability of animals. When applied to antifungal feed, it has good moisture and heat resistance, is less affected by pH, and has the effect of efficiently inhibiting and killing microorganisms such as bacteria and fungi in feed.

In addition, there are also research reports that the above components are used to prepare feed additives that can replace antibiotics, such as:

The invention patent application document with the application publication number CN104839516A discloses a compound poultry feed, which is composed of the following components in parts by weight: 20-50 parts of corn, 15-21 parts of soybean meal, 10-35 parts of wheat bran, vegetable 5-20 parts of seed meal, 3-10 parts of secondary powder, 4-6 parts of distiller's grain powder, 3-6 parts of rice bran, 2-8 parts of feed grade jujube powder, 4-8 parts of fish meal, 1-5 parts of spirulina powder, 3-5 parts of traditional Chinese medicine additives, 0.5-5 parts of shell powder, 1-3.5 parts of calcium powder, 0.2-1 part of brown sugar, 0.55-0.75 parts of bone powder, 0.3-0.4 parts of table salt, 0.1-0.4 parts of feed additives, 0.1 parts of trace element additives ～0.3 parts, 0.3～0.5 parts of multivitamins, 0.2～0.6 parts of glycerol monolaurate. The feed can promote the formation and enrichment of organic trace elements in poultry and eggs, and reduce the use of antibiotic drugs and additives and drug residues in poultry products.

The invention patent application document with the application publication number CN103005210A discloses a pig feed additive that can replace antibiotics and its preparation method and application. , 1-5 parts of angelica, 1-5 parts of astragalus, 1-5 parts of hawthorn, 1-5 parts of Codonopsis, 10-20 parts of citric acid, 10-20 parts of lactic acid, 20-30 parts of coated phosphoric acid, 5 parts of probiotics ~10 parts, 5-10 parts of yeast cell wall polysaccharide, 1-5 parts of compound plant essential oil and 5-10 parts of carrier. Wherein, the coated phosphoric acid is a mixture of mono/distearic acid glyceride and phosphoric acid. The feed additive can completely replace antibiotics and solve many problems such as antibiotic resistance, drug residues, and overuse.

The invention patent application document with the application publication number CN102696883A discloses a feed additive and its application. The feed additive includes: 20-60% glyceryl monolaurate, 10-40% calcium propionate, 0- 50% feed carrier. The feed additive can not only effectively inhibit the growth and reproduction of mold, prolong the storage period of feed in hot and humid seasons, but also have the functions of antibacterial and antiviral, reducing the use of antibiotics in feed, promoting animal growth, improving animal survival rate and disease resistance.

However, mechanistic studies have found that the additives used as antibiotic substitutes need to consider issues such as palatability, intestinal environment balance, and intestinal flora balance. If used improperly, it may cause performance decline, intestinal environment imbalance, and bacterial flora imbalance; The feed additives disclosed in the above documents that can be used to replace antibiotics can replace antibiotics to a certain extent, but there are problems such as the feed additive has too much or too little bactericidal effect in livestock and poultry, which affects the growth of livestock and poultry. The replacement effect of antibiotics is not good, and it still needs to be improved, and the influence of the ratio of lauric acid mono/diglycerides on the balance of the intestinal environment and the balance of intestinal flora has not been considered.

Contents of the invention

The invention provides a feed additive that can replace antibiotics and its application. Adding the feed additive in piglet breeding can replace the use of antibiotics, improve the disease resistance of piglets, promote the development of animal intestines and the improvement of intestinal functions, and improve daily life. Increase weight and feed intake, reduce diarrhea rate and death rate, improve feed conversion rate, promote animal growth and development, improve breeding economic benefits, reduce antibiotic use, and reduce environmental pollution.

Technical scheme of the present invention is as follows:

A feed additive, by weight percentage, comprising:

The present invention aims at the influence of the mono/diglyceride content and ratio of lauric acid on the palatability, the balance of the intestinal environment, the balance of the intestinal flora, and the emulsification that helps the digestion and absorption of intestinal nutrients, and optimizes the obtained An animal feed antibiotic substitute formula with food-attracting effect, little irritation to the gastrointestinal tract, and inhibiting harmful bacteria from invading the intestinal tract without causing excessive inhibition of intestinal flora.

After the above-mentioned feed additives enter the intestinal tract of the piglets, on the one hand, they use the antibacterial and antiviral effects of glyceryl monolaurate and lauric acid derived from glyceryl dilaurate to build an exogenous protective barrier in the intestinal tract. Kill exogenous pathogenic bacteria invading the intestinal tract, improve the health level of farmed animals, and provide protection for the improvement of the immune system of young animals; Energy, promotes gut health.

The specific ratio of glyceryl monolaurate and glyceryl dilaurate can make the taste of the feed additive good. For piglets, it has a good food-inducing effect and increases feed intake and daily gain.

Glyceryl monostearate at an appropriate dose can emulsify feed ingredients, promote digestion and absorption in the intestine, and promote the formation of chyme microns. Moreover, it has been found through experiments that glyceryl monostearate can further promote the antibacterial and antiviral effects of glyceryl monolaurate and glyceryl dilaurate, and for piglets, improve feed intake and daily gain, enhance disease resistance.

More preferably, said feed additive, by weight percentage, includes:

Further preferably, said feed additive, by weight percentage, includes:

More preferably, said feed additive, by weight percentage, includes:

Preferably, the carrier is silicon dioxide, maltodextrin or starch. The above-mentioned carrier is beneficial to the dispersion of glyceryl mono/dilaurate and glyceryl monostearate in the feed, and does not react with them, which is beneficial to the play of the substitution effect of antibiotics.

The present invention also provides an animal feed, which contains the above-mentioned feed additive. The animal feed can be directly applied to animal breeding, can replace half or all of the antibiotics used in feeding, improves the immunity and survival rate of animals, and promotes the growth of animals.

The antibiotics include: colistin sulfate and florfenicol.

The animal feed is livestock feed. Specifically, the livestock feed is pig feed.

Preferably, in weight percentage, the added amount of the feed additive in the animal feed is 0.05-0.3%.

The dosage of glyceryl monolaurate, glyceryl dilaurate and glyceryl monostearate in the feed additive of the present invention must be appropriate, otherwise the feed additive with too high content will cause feed intake due to astringent taste and strong antibacterial and antiviral effects. Feed additives with low content and the imbalance of intestinal flora structure will lead to the decrease of daily weight gain, while feed additives with too low content will not be able to build a protective barrier in the intestinal tract due to insufficient dosage, and will have little effect on improving feed intake and daily gain. smaller.

As a preference, the animal feed includes feed material and the feed additive; in weight percentage, the formula of the feed material is: 30.0-65.0% of corn, 9.0-14.0% of wheat, 4.0-6.0% of secondary flour, and 16-20% soybean meal, 6.0-8.0% puffed soybean, 10.0-14% high lactose whey powder, 3.0-6.0% imported fish meal, 1.0-3.0% skimmed milk powder, 1.0-5.0% premix, 1.0-3.0% stone powder %;

In terms of weight percentage, the nutritional components of the feed raw materials are: crude protein 16-23%, lysine 0.8-1.6%, methionine 0.5-0.8%, cystine 0.3-0.6%, threonine 0.8-1.2% , tryptophan 0.2-0.4%, total calcium 1.0-2.0%, total phosphorus 0.8-1.0%, available phosphorus 0.4-0.6%.

More preferably, when the animal feed is feed for piglets, the formula of the feed raw materials is: by weight percentage, corn 36.0%, wheat 12.0%, secondary flour 5.0%, peeled soybean meal 17.5%, puffed soybean 8.0% , high lactose whey powder 12.5%, imported fish meal 5.0%, skimmed milk powder 2.0%, pig premix 1.0%, stone powder 1.0%;

In terms of weight percentage, the nutritional ingredients of the feed raw materials are: crude protein 22%, lysine 1.4%, methionine 0.6%, cystine 0.4%, threonine 0.9%, tryptophan 0.3%, total calcium 1.0% %, total phosphorus 0.85%, available phosphorus 0.5%.

The present invention also provides a method for preparing animal feed, comprising: adding the feed additive to feed raw materials, and preparing the animal feed by stirring and mixing or extruding and granulating. The extrusion granulation can be carried out using a granulator.

The invention also provides the application of the feed additive as an antibiotic substitute in piglet feed.

Compared with the prior art, the present invention has the following beneficial effects:

(1) The present invention takes glyceryl monolaurate, glyceryl dilaurate and glyceryl monostearate as main raw materials, and the feed additive prepared according to the appropriate content and ratio can replace antibiotics in half or full amount in piglet breeding. When used, it has the effect of inhibiting the invasion of harmful bacteria on the intestinal tract without causing excessive inhibition of intestinal flora. At the same time, glyceryl dilaurate slowly releases lauric acid under the action of intestinal flora and digestive enzymes, which has little irritation to the gastrointestinal tract. , and play the role of continuous and rapid energy supply and antibacterial and antiviral, increase the effect of attracting food, promote the growth and development of animals, improve the economic benefits of breeding, reduce the use of antibiotics, thereby reducing environmental pollution.

(2) The feed additive of the present invention can improve the disease resistance of piglets without using antibiotics, reduce the probability of getting sick, improve the survival rate, reduce diseases such as diarrhea, and stimulate the fermentation of intestinal flora to produce acetic acid, propionic acid, butyric acid , promote intestinal development and intestinal function improvement.

(3) The feed additive of the present invention can also promote the emulsification of other components of feed raw materials, promote the formation of chyme particles, enhance the catabolism of feed protein and carbohydrates by intestinal flora, promote the digestion and absorption of nutrients, and accelerate the growth of piglets.

(4) The feed additive of the present invention is a natural, green and safe feed additive, which will not cause drug resistance in animals, has no drug residue and pollution, ensures the quality, safety and harmlessness of meat products, and has better flavor, bringing more green to pig breeding , Security protection, great application value.

detailed description

The following is a clear and complete description of the technical solutions in the present invention in conjunction with specific embodiments, and all other simple deformations or modifications made without departing from the core of the present invention fall within the protection scope of the present invention.

In the following examples, the preparation method of the feed is as follows:

(1) Uniformly pulverize each raw material, and then mix uniformly according to the ratio to obtain the feed additive;

(2) Add feed additives to the feed raw materials according to a certain amount, fully mix them with a horizontal steel-wing double-screw mixer, and then send them to the bucket elevator through a belt conveyor, and then pass the material through the bucket elevator Send it to the granulator, and under the action of the pressure roller and template inside the granulator, the material is extruded into granules to make feed.

Among them, the formula of feed raw materials is: by weight percentage, corn 36.0%, wheat 12.0%, secondary flour 5.0%, peeled soybean meal 17.5%, puffed soybean 8.0%, high lactose whey powder 12.5%, imported fish meal 5.0%, Skimmed milk powder 2.0%, pig premix 1.0%, stone powder 1.0%;

The nutrient composition of described feed raw material is: by weight percentage, crude protein 22%, lysine 1.4%, methionine 0.6%, cystine 0.4%, threonine 0.9%, tryptophan 0.3%, total calcium 1.0% %, total phosphorus 0.85%, available phosphorus 0.5%.

The formulations of the feed ingredients mentioned in the following examples are the same.

The above-mentioned feed additive refers to the antibiotic feed additive, antibiotic or the mixture of antibiotic feed additive and antibiotic according to the present invention.

Example 1

According to the principle of similar body weight and similar genetic basis, 240 weaned piglets (half male and female) at the age of 23 days were selected and randomly divided into 4 groups: the test group and the control group 1-3, each group had 3 replicates, and each replicated 20 piglets Be a column; Feed weaned piglets with piglet feed, and the test period is 28 days.

The composition and formula of piglet feed in above-mentioned test group and control group 1～3 are as follows:

(1) Test group (replacing antibiotics in full): Substituting antibiotics feed additive + feed raw materials

The formula of the substitute antibiotic feed additive is: by weight percentage, 15% of glyceryl monolaurate, 30% of glyceryl dilaurate, 30% of glyceryl monostearate and 25% of carrier (silicon dioxide). The addition amount of alternative antibiotic feed additive in piglet feed is 0.1%.

(2) Control group 1 (blank control): no substitute antibiotic feed additive was added, and only feed raw materials were used.

(3) Control group 2 (half-quantity replacement of antibiotics): substitute antibiotics feed additive + antibiotics + feed ingredients

The formula of the substitute antibiotic feed additive is: by weight percentage, 15% of glyceryl monolaurate, 30% of glyceryl dilaurate, 30% of glyceryl monostearate and 25% of carrier (silicon dioxide).

Antibiotic: colistin sulfate.

The addition amount of alternative antibiotic feed additive in piglet feed is 0.1%; the addition amount of antibiotic in piglet feed is 50mg/kg.

(4) Control group 3 (antibiotic group): antibiotic + feed material

Antibiotic: colistin sulfate.

The amount of antibiotics added to piglet feed was 100mg/kg.

At the end of the experiment, fresh stool samples were collected to measure the diversity of fecal flora and the content of short-chain fatty acids. The result is as follows:

Data recording and effect evaluation indicators (see Table 1 and Table 2)

Table 1. Feeding effect of weaned piglets

Note: The data are means±SD; different lowercase letters in the same column represent significant differences, P﹤0.05.

Table 2 Effects of fecal flora diversity and short-chain fatty acids on weaned piglets

Note: The number of observed species and the Shannon index are indicators to characterize the diversity of the flora; the data are means±SD; different lowercase letters in the same column represent significant differences, P﹤0.05.

From Table 1-2, it can be seen that the weight gain and feed intake of the test group and the control group were significantly higher than those of the control group 1 and the control group 3, especially the weight gain and feed intake of the control group were higher than those of the control group 3. 19.7% and 12.8%, and the feed-to-meat ratio, diarrhea rate, and death rate also dropped significantly.

Compared with the control group 3, the daily gain of the piglets in the test group increased by 5%, and the death rate was zero, indicating that the use of the feed additive in the test group 1 has a better effect than colistin sulfate, and greatly reduces the amount of colistin sulfate. The use of mycocin antibiotics makes the feed more efficient and safer.

At the same time, the number of intestinal flora and the Shannon index of the experimental group and the control group 2 were higher than that of the control group 1 and lower than that of the control group 3, indicating that the feed additives in the experimental group had a good inhibitory effect on the intestinal flora, but not It leads to the imbalance of intestinal flora, which comprehensively shows that the feed additives in the experimental group are more efficient and safer, which can effectively improve the disease resistance of piglets and promote the growth of piglets.

Example 2

The experiment adopts single factor design, according to the principle of similar body weight and similar genetic basis, select 240 weaned piglets (half male and female) at the age of 28 days, and randomly divide them into 4 groups: control group, treatment group A, treatment group B, treatment group Group C, 3 repetitions in each group, 20 weaned piglets in each repetition; the weaned piglets were fed with piglet feed, and the test period was 60 days.

The formula of the piglet feed in the above-mentioned control group and treatment groups A～C is specifically as follows:

(1) Control group:

Feeding method: Only feed antibiotics and feed ingredients in the first and fifth weeks of the test period, and feed piglets with only feed ingredients as piglet feed for the rest of the time.

Antibiotics (Florfenicol): The amount added to piglet feed is 100mg/kg.

(2) Treatment group A:

Feeding method: Only feed the mixture of antibiotics, alternative antibiotic feed additives and feed ingredients in the first and fifth weeks of the test period, and only use alternative antibiotic feed additives and feed ingredients as piglet feed for the rest of the time.

Among them, antibiotics (florfenicol): the addition amount in the first week was 100 mg/kg, and the addition amount in the fifth week was 50 mg/kg.

The formulation of the substitute antibiotic feed additive is as follows: by weight percentage, 20% of glyceryl monolaurate, 40% of glyceryl dilaurate, 30% of glyceryl monostearate and 10% of carrier (maltodextrin). The addition amount of alternative antibiotic feed additive in piglet feed is 0.1%.

(3) Treatment group B:

Feeding method: Only feed the mixture of antibiotics, alternative antibiotic feed additives and feed ingredients in the first and fifth weeks of the test period, and only use alternative antibiotic feed additives and feed ingredients as piglet feed for the rest of the time.

Among them, antibiotics (florfenicol): the addition amount in the first week was 50 mg/kg, and the addition amount in the fifth week was 50 mg/kg.

The formulation of the substitute antibiotic feed additive is as follows: by weight percentage, 20% of glyceryl monolaurate, 40% of glyceryl dilaurate, 30% of glyceryl monostearate and 10% of carrier (maltodextrin). The addition amount of alternative antibiotic feed additive in piglet feed is 0.1%.

(4) Treatment group C:

Feeding method: Only substitute antibiotic feed additives and feed raw materials are used as piglet feed to feed piglets throughout the whole process.

The addition amount of alternative antibiotic feed additive in piglet feed is 0.1%. The formulation of the substitute antibiotic feed additive is as follows: by weight percentage, 20% of glyceryl monolaurate, 40% of glyceryl dilaurate, 30% of glyceryl monostearate and 10% of carrier (maltodextrin).

Data recording and effect evaluation indicators (see Table 3)

Table 3. Feeding effect of weaned piglets

Note: The data are Mean±SD, different lowercase letters in the same row represent significant differences, P﹤0.05.

It can be seen from Table 3 that the daily gain and feed intake of piglets in treatment groups A, B and C increased by 12.3% to 14.9% and 6.7% to 10.8% respectively compared with the control group (ie, the full antibiotic group). The rate also decreased significantly, indicating that the use of alternative antibiotic feed additives has a better effect than florfenicol, and greatly reduces the use of florfenicol antibiotics. The feed produced is more efficient and safe, and can effectively improve the disease resistance of piglets Ability to promote piglet growth.

Example 3

The experiment adopts single factor design, and according to the principle of similar body weight and similar genetic basis, 300 weaned piglets (half male and female) at the age of 28 days were selected and randomly divided into 5 groups: treatment group, control group I, control group II, control group Group III and control group IV, 3 repetitions in each group, 20 heads in each repetition, fed weaned piglets with piglet feed, and the test period was 30 days.

The formula of the piglet feed in above-mentioned treatment group and control group I～IV is specific as follows:

(1) Treatment group: alternative antibiotic feed additive + feed raw materials

The formula of the substitute antibiotic feed additive is: by weight percentage, 20% of glyceryl monolaurate, 30% of glyceryl dilaurate, 30% of glyceryl monostearate and 20% of carrier (silicon dioxide).

The addition amount of alternative antibiotic feed additive in piglet feed is 0.1%.

(2) Control group I: Substitute antibiotic feed additive + feed material

Only glyceryl monolaurate was used as a feed additive to replace antibiotics, and its addition amount in piglet feed was the same as that of glyceryl monolaurate in the treatment group, ie 0.02%.

(3) Control group II: Substitute antibiotic feed additive + feed material

Only the mixture of glyceryl monolaurate, glyceryl monostearate and carrier (silicon dioxide) was used as a substitute antibiotic feed additive. Same, namely: glyceryl monolaurate 0.02%, glyceryl monostearate 0.03%, carrier (silicon dioxide) 0.02%.

(4) Control group III: alternative antibiotic feed additive + feed material

Only the mixture of glyceryl monolaurate, glyceryl dilaurate and carrier (silicon dioxide) was used as a substitute antibiotic feed additive, and the addition amount of each component in piglet feed was the same as that in the treatment group , namely: 0.02% glyceryl monolaurate, 0.03% glyceryl dilaurate, and 0.02% carrier (silicon dioxide).

(5) Control group IV: alternative antibiotic feed additive + feed raw materials

Only the mixture of glyceryl monolaurate, glyceryl dilaurate and glyceryl monostearate was used as a substitute antibiotic feed additive, and the addition amount of each component in piglet feed was the same as that in the treatment group , namely: glyceryl monolaurate 0.02%, glyceryl dilaurate 0.03%, glyceryl monostearate 0.03%.

Data recording and effect evaluation indicators (see Table 4)

Table 4. Feeding effect of weaned piglets

Note: The data are Mean±SD, different lowercase letters in the same row represent significant differences, P﹤0.05.

It can be seen from Table 4 that the daily gain and feed intake of the treatment group increased by 9.3% and 6.8%, 7.2% and 6.4%, 2.8% and 2.0% respectively compared with the control group I, control group II, control group III and control group IV , 1.2% and 0.4%, compared with the control group I and control group II, the feed-to-meat ratio and the death rate were also significantly reduced, indicating that both glyceryl dilaurate and glyceryl monostearate can effectively improve the disease resistance of piglets. Promote the growth of piglets, and the effect of glyceryl dilaurate is greater than that of glyceryl monostearate, comprehensively indicating that glyceryl monolaurate is combined with glyceryl dilaurate, glyceryl monostearate and carrier (silicon dioxide) and After adopting a suitable dose ratio, the use effect is significantly better than that of monolaurin alone, and it is more efficient and safe. It can effectively improve the disease resistance of piglets, promote the growth of piglets, and obtain better economic benefits.

Example 4

The experiment adopts single factor design, according to the principle of similar body weight and similar genetic basis, select 300 weaned piglets (half male and female) at the age of 28 days, and randomly divide them into 5 groups: treatment group, control group D, control group E, control group Group F, control group G, 3 repetitions in each group, 20 piglets in each repetition, fed weaned piglets with piglet feed respectively, and the test period was 30 days.

(1) Treatment group: alternative antibiotic feed additive + feed raw materials

The formulation of the substitute antibiotic feed additive is as follows: by weight percentage, 20% of glyceryl monolaurate, 30% of glyceryl dilaurate, 30% of glyceryl monostearate and 20% of carrier (maltodextrin).

The addition amount of the substitute antibiotic feed additive in the piglet feed is 0.1%, that is, the weight percentage of monolaurin in the piglet feed is 0.02%.

(2) Control group D: alternative antibiotic feed additive + feed material

With the application publication number being the formula of Example 1 in the invention patent application document of CN102696883A as the substitute antibiotic feed additive.

Specifically: in terms of weight percentage, the weight ratio of glyceryl monolaurate, calcium propionate and feed carrier (maltodextrin) with a monoester content of 70% is 3:2:5, and the substitute antibiotic feed additive is used in piglet feed The added amount is 0.1%.

(3) Control group E: alternative antibiotic feed additive + feed raw materials

The mixture of glyceryl monolaurate, glyceryl dilaurate, glyceryl monostearate and carrier (maltodextrin) is used as a substitute antibiotic feed additive, and the weight of glyceryl monolaurate is adjusted to 1/4 times of the treatment group (ie 0.005%), and the addition amount of each component in the feed for piglets is the same as the addition amount of each component in the treatment group.

(4) Control group F: alternative antibiotic feed additive + feed material

The mixture of glyceryl monolaurate, glyceryl dilaurate, glyceryl monostearate and carrier (maltodextrin) is used as the substitute antibiotic feed additive, and the weight of glyceryl monolaurate is adjusted to be 1.5 times of the treatment group (i.e. 0.03%), and the addition amount of each component in piglet feed was the same as that of each component in the treatment group.

(5) Control group G: alternative antibiotic feed additive + feed material

The mixture of glyceryl monolaurate, glyceryl dilaurate, glyceryl monostearate and carrier (maltodextrin) is used as a substitute antibiotic feed additive, and the weight of glyceryl monolaurate is adjusted to be 3 times of the treatment group (i.e. 0.06%), and the addition amount of each component in piglet feed was the same as that of each component in the treatment group.

Data recording and effect evaluation indicators (see Table 5)

Table 5. Feeding effect of weaned piglets

Note: The data are Mean±SD, different lowercase letters in the same row represent significant differences, P﹤0.05.

It can be seen from Table 5 that the daily gain and feed intake of the treatment group were significantly higher than that of the control group D, which increased by 15.1% and 12.6% respectively, indicating that the use effect of the feed additive in the treatment group was significantly better than that of the invention whose application publication number is CN102696883A The formula in the patent application literature, and the prepared feed is more efficient, which can more effectively improve the disease resistance of piglets and promote the growth of piglets. Compared with control group E, control group F and control group G, the daily gain and feed intake of the treatment group increased by 27.1% and 22.5%, 4.6% and 3.8%, 9.8% and 6.7%, respectively. Also significantly decreased, illustrating that the ratio of glyceryl monolaurate, glyceryl dilaurate and glyceryl monostearate must be suitable in the feed additives of the embodiment treatment group, and the consumption of glyceryl monolaurate must be suitable and reasonable, otherwise the content Feed additives that are too high will have a lesser effect on increasing feed intake due to astringent taste and strong antibacterial and antiviral effects, and the structure of intestinal flora will be imbalanced, resulting in a decrease in daily weight gain. It cannot build a protective barrier in the intestinal tract, and has little effect, and has little effect on improving feed intake and daily gain.

In short, the feed additive of the present invention is compounded according to the appropriate content and proportion, and added to piglet feed, which can replace the use of antibiotics in piglet breeding in half or full amount, and can inhibit the invasion of harmful bacteria on the intestinal tract without causing excessive inhibition of intestinal flora The role of lauric acid is slowly released under the action of intestinal flora and digestive enzymes, which has little irritation to the gastrointestinal tract, and plays a continuous and rapid energy supply, antibacterial and antiviral effect, and stimulates the fermentation of intestinal flora to produce acetic acid and propionic acid , butyric acid, reduce piglet diarrhea and other diseases, promote intestinal development and intestinal function improvement, improve disease resistance of piglets, reduce disease probability, improve survival rate, increase food-attracting effect, promote growth and development, and improve breeding economic benefits. Reduce the use of antibiotics and reduce environmental pollution.

Claims (10)

1. A feed additive, characterized in that, in weight percentage, comprising: 2. feed additive as claimed in claim 1, is characterized in that, by weight percentage, comprises: 3. The feed additive according to claim 1 or 2, characterized in that the carrier is silicon dioxide, maltodextrin or starch. 4. An animal feed, characterized in that it contains the feed additive according to any one of claims 1-3. 5. The animal feed according to claim 4, characterized in that, in weight percentage, the added amount of said feed additive in the animal feed is 0.05-0.3%. 6. The animal feed according to claim 5, characterized in that, the animal feed is pig feed. 7. The animal feed according to claim 5, characterized in that, the animal feed comprises feed materials and the feed additive; the formula of the feed materials is: by weight percentage, corn 30.0-65.0%, wheat 9.0～14.0%, secondary powder 4.0～6.0%, peeled soybean meal 16～20%, puffed soybean 6.0～8.0%, high lactose whey powder 10.0～14%, imported fish meal 3.0～6.0%, skimmed milk powder 1.0～3.0% , premix 1.0-5.0%, stone powder 1.0-3.0%; The nutritional components of the feed raw materials are: by weight percentage, crude protein 16-23%, lysine 0.8-1.6%, methionine 0.5-0.8%, cystine 0.3-0.6%, threonine 0.8-1.2% , tryptophan 0.2-0.4%, total calcium 1.0-2.0%, total phosphorus 0.8-1.0%, available phosphorus 0.4-0.6%. 8. The animal feed according to claim 7, characterized in that, the animal feed is piglet feed; the formula of the feed raw material is: by weight percentage, 36.0% of corn, 12.0% of wheat, and 5.0% of secondary flour , peeled soybean meal 17.5%, puffed soybean 8.0%, high lactose whey powder 12.5%, imported fish meal 5.0%, skimmed milk powder 2.0%, pig premix 1.0%, stone powder 1.0%; The nutrient composition of described feed raw material is: by weight percentage, crude protein 22%, lysine 1.4%, methionine 0.6%, cystine 0.4%, threonine 0.9%, tryptophan 0.3%, total calcium 1.0% %, total phosphorus 0.85%, available phosphorus 0.5%. 9. A method for preparing animal feed, characterized in that the feed additive according to any one of claims 1 to 4 is added to feed raw materials, and the animal feed is prepared by stirring and mixing or extrusion granulation. 10. The application of the feed additive according to any one of claims 1 to 3 as an antibiotic substitute in piglet feed.