CN105165815A - Root dipping method of low concentration BA and glycerin gel - Google Patents
Root dipping method of low concentration BA and glycerin gel Download PDFInfo
- Publication number
- CN105165815A CN105165815A CN201510525554.8A CN201510525554A CN105165815A CN 105165815 A CN105165815 A CN 105165815A CN 201510525554 A CN201510525554 A CN 201510525554A CN 105165815 A CN105165815 A CN 105165815A
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- CN
- China
- Prior art keywords
- hormone
- concentration
- root
- working solution
- low concentration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 title claims abstract description 18
- 235000011187 glycerol Nutrition 0.000 title claims abstract description 9
- 238000007598 dipping method Methods 0.000 title abstract 3
- 238000000034 method Methods 0.000 claims abstract description 14
- 239000012452 mother liquor Substances 0.000 claims abstract description 13
- 239000007788 liquid Substances 0.000 claims abstract description 6
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 5
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000001768 carboxy methyl cellulose Substances 0.000 claims abstract description 5
- 239000000661 sodium alginate Substances 0.000 claims abstract description 5
- 235000010413 sodium alginate Nutrition 0.000 claims abstract description 5
- 229940005550 sodium alginate Drugs 0.000 claims abstract description 5
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 claims abstract description 5
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 claims abstract description 5
- 239000005556 hormone Substances 0.000 claims description 26
- 229940088597 hormone Drugs 0.000 claims description 26
- 239000012224 working solution Substances 0.000 claims description 16
- 238000002360 preparation method Methods 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 239000012153 distilled water Substances 0.000 claims description 3
- 239000011159 matrix material Substances 0.000 claims description 3
- 239000012530 fluid Substances 0.000 abstract 2
- 238000007865 diluting Methods 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 4
- 235000013399 edible fruits Nutrition 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 2
- UDPGUMQDCGORJQ-UHFFFAOYSA-N (2-chloroethyl)phosphonic acid Chemical compound OP(O)(=O)CCCl UDPGUMQDCGORJQ-UHFFFAOYSA-N 0.000 description 1
- ZMZGFLUUZLELNE-UHFFFAOYSA-N 2,3,5-triiodobenzoic acid Chemical compound OC(=O)C1=CC(I)=CC(I)=C1I ZMZGFLUUZLELNE-UHFFFAOYSA-N 0.000 description 1
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 1
- 241001656898 Buxus microphylla Species 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- KNXVOGGZOFOROK-UHFFFAOYSA-N trimagnesium;dioxido(oxo)silane;hydroxy-oxido-oxosilane Chemical compound [Mg+2].[Mg+2].[Mg+2].O[Si]([O-])=O.O[Si]([O-])=O.[O-][Si]([O-])=O.[O-][Si]([O-])=O KNXVOGGZOFOROK-UHFFFAOYSA-N 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a root dipping method of low concentration BA and glycerin gel, and belongs to the technical field of plant biology. The method comprises the following steps: at first, preparing a BA mother liquor with a concentration of 1 mg/mL, diluting the mother liquid into a low concentration BA work fluid with a concentration of 10 mg/L, using the work fluid to prepare a low concentration BA and glycerin gel confining liquid, which contains sodium carboxymethyl cellulose with a mass concentration of 1% and sodium alginate with a mass concentration of 8%, dipping the lower ends of healthy and robust branches into the gel confining liquid for 3 to 5 seconds, taking out the branches, and inserting the branches into a cultivation medium to make the branches grow out roots.
Description
Technical field
Low concentration BA and glycerogel be stained with a method for root, belong to plant biotechnology field.
Background technology
Cottage propagation is one of vegetative common method of plant, especially trees, and the method has that cost is low, instant effect, cycle short, used branch abundance and can keep the plurality of advantages such as parent's merit.Usually can use dissimilar hormone during cottage propagation, as BA, IBA, NAA, 2,4-D, GA
3, 6-BA, KT, TDZ, TIBA, ethrel, B
9, PP333, MH etc., the interpolation of above-mentioned hormone can enable plant at body or in vitro ahead of time or postpone to take root, bloom, bear fruit, and increases radical, induction axillary bud sprouting, improves percentage of fertile fruit etc., agricultural production plays important purposes.But the branch being difficult to break up in in-vitro propagate or rooting process, due to the loss of long moisture or nutriment, extremely easily causes death.And the method that existing employing urges root to lure bud to bloom possesses skills the shortcomings such as step is more, cost is high, unstable, in production, effect is unsatisfactory.Therefore, need badly a kind of fast and convenient method for plant axillary bud deriving, take root and Cheng Hua etc.
Summary of the invention
Low concentration BA and glycerogel be stained with a method for root, it is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel in described method, be stained with root, cuttage, concrete operation step is as follows:
1. hormone mother liquor: precise weight is, after the KOH of a little 1M of BA of 0.1g dissolves, be settled to 100mL, be the BA mother liquor of 1mg/mL with distilled water;
2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:100 the low concentration hormone working solution that concentration is 10mg/L, adds 10ml glycerine according to 90ml hormone working solution simultaneously;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 10mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;
be stained with root: branch lower end sturdy for health is sticked in 3-5 in above-mentioned gel confining liquid and take out after second;
cuttage: above-mentioned branch is inserted in cultivation matrix and takes root.
The invention has the advantages that from the viewpoint of water conservation, short root, lure bud, bloom, bear fruit, with hormone working solution for solvent, sodium carboxymethylcellulose and sodium alginate are gel carrier, are mixed with containing gel solution that is dissimilar, concentration hormone.Features such as during use, branch, flower being soaked in slightly above-mentioned solution or above-mentioned solution is dripped at axillalry bud place, film can be formed rapidly after gel drying, reaching the evaporation of minimizing moisture, being continuously applied hormone, be easy and simple to handle.
embodiment:
Below in conjunction with specific embodiment, the present invention is conducted further description.
Embodiment
Low concentration BA and glycerogel be stained with a method for root, it is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel in described method, be stained with root, cuttage, concrete operation step is as follows:
1. hormone mother liquor: precise weight is, after the KOH of a little 1M of BA of 0.1g dissolves, be settled to 100mL, be the BA mother liquor of 1mg/mL with distilled water;
2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:100 the low concentration hormone working solution that concentration is 10mg/L, simultaneously add 10ml glycerine according to 90ml hormone working solution;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 10mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;
be stained with root: 8-10cm is long, healthy sturdy Chinese littleleaf box branch lower end sticks in 3-5 in above-mentioned gel confining liquid and takes out after second;
cuttage: inserted in cultivation matrix by above-mentioned branch and take root, after 30-40 days, rooting rate reaches more than 75%.
Claims (1)
1. low concentration BA and glycerogel be stained with a method for root, it is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel in described method, be stained with root, cuttage, concrete operation step is as follows:
1. hormone mother liquor: precise weight is, after the KOH of a little 1M of BA of 0.1g dissolves, be settled to 100mL, be the BA mother liquor of 1mg/mL with distilled water;
2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:100 the low concentration hormone working solution that concentration is 10mg/L, adds 10ml glycerine according to 90ml hormone working solution simultaneously;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 10mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;
be stained with root: branch lower end sturdy for health is sticked in 3-5 in above-mentioned gel confining liquid and take out after second;
cuttage: above-mentioned branch is inserted in cultivation matrix and takes root.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510525554.8A CN105165815A (en) | 2015-08-25 | 2015-08-25 | Root dipping method of low concentration BA and glycerin gel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510525554.8A CN105165815A (en) | 2015-08-25 | 2015-08-25 | Root dipping method of low concentration BA and glycerin gel |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105165815A true CN105165815A (en) | 2015-12-23 |
Family
ID=54888694
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510525554.8A Pending CN105165815A (en) | 2015-08-25 | 2015-08-25 | Root dipping method of low concentration BA and glycerin gel |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105165815A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101238806A (en) * | 2008-03-14 | 2008-08-13 | 郑永昌 | Fruit tree protection glue for preventing fruit tree disease scar and wound generated by pruning from rot |
| CN102599161A (en) * | 2012-02-09 | 2012-07-25 | 濮阳市林业科学院 | Hormone hydrogel rooting film |
| WO2015038917A1 (en) * | 2013-09-13 | 2015-03-19 | Fine Agrochemicals Ltd. | Plant growth regulating composition and methods for making and using same |
-
2015
- 2015-08-25 CN CN201510525554.8A patent/CN105165815A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101238806A (en) * | 2008-03-14 | 2008-08-13 | 郑永昌 | Fruit tree protection glue for preventing fruit tree disease scar and wound generated by pruning from rot |
| CN102599161A (en) * | 2012-02-09 | 2012-07-25 | 濮阳市林业科学院 | Hormone hydrogel rooting film |
| WO2015038917A1 (en) * | 2013-09-13 | 2015-03-19 | Fine Agrochemicals Ltd. | Plant growth regulating composition and methods for making and using same |
Non-Patent Citations (1)
| Title |
|---|
| 张溃珍等: "高分子•激素水凝胶生根膜制备与试验", 《林业实用技术》 * |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20151223 |
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| RJ01 | Rejection of invention patent application after publication |