CN105010320A - Node position use method of middle-concentration IBA and glycerol gel - Google Patents
Node position use method of middle-concentration IBA and glycerol gel Download PDFInfo
- Publication number
- CN105010320A CN105010320A CN201510525515.8A CN201510525515A CN105010320A CN 105010320 A CN105010320 A CN 105010320A CN 201510525515 A CN201510525515 A CN 201510525515A CN 105010320 A CN105010320 A CN 105010320A
- Authority
- CN
- China
- Prior art keywords
- hormone
- iba
- working solution
- concentration
- mother liquor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title claims abstract description 16
- 239000012224 working solution Substances 0.000 claims abstract description 18
- 239000012452 mother liquor Substances 0.000 claims abstract description 14
- 235000011187 glycerol Nutrition 0.000 claims abstract description 8
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 5
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000001768 carboxy methyl cellulose Substances 0.000 claims abstract description 5
- 239000000661 sodium alginate Substances 0.000 claims abstract description 5
- 235000010413 sodium alginate Nutrition 0.000 claims abstract description 5
- 229940005550 sodium alginate Drugs 0.000 claims abstract description 5
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 claims abstract description 5
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 claims abstract description 5
- 239000005556 hormone Substances 0.000 claims description 26
- 229940088597 hormone Drugs 0.000 claims description 26
- 238000002360 preparation method Methods 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 239000011159 matrix material Substances 0.000 claims description 3
- 239000000243 solution Substances 0.000 abstract description 5
- 238000007789 sealing Methods 0.000 abstract 2
- 238000007865 diluting Methods 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 4
- 235000013399 edible fruits Nutrition 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 2
- UDPGUMQDCGORJQ-UHFFFAOYSA-N (2-chloroethyl)phosphonic acid Chemical compound OP(O)(=O)CCCl UDPGUMQDCGORJQ-UHFFFAOYSA-N 0.000 description 1
- ZMZGFLUUZLELNE-UHFFFAOYSA-N 2,3,5-triiodobenzoic acid Chemical compound OC(=O)C1=CC(I)=CC(I)=C1I ZMZGFLUUZLELNE-UHFFFAOYSA-N 0.000 description 1
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 1
- 240000001432 Calendula officinalis Species 0.000 description 1
- 235000005881 Calendula officinalis Nutrition 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- KNXVOGGZOFOROK-UHFFFAOYSA-N trimagnesium;dioxido(oxo)silane;hydroxy-oxido-oxosilane Chemical compound [Mg+2].[Mg+2].[Mg+2].O[Si]([O-])=O.O[Si]([O-])=O.[O-][Si]([O-])=O.[O-][Si]([O-])=O KNXVOGGZOFOROK-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention discloses a node position use method of middle-concentration IBA and glycerol gel, and belongs to the field of plant biotechnology. The method comprises the following steps: first, preparing a 1 mg/mL IBA mother liquor; diluting the mother liquor into a 100 mg/L middle-concentration IBA working solution; preparing a middle-concentration IBA and glycerol gel sealing solution which comprises 1 mass percent of sodium carboxymethyl cellulose and 8 mass percent of sodium alginate from the working solution; inserting a healthy thick branch in a cultivation medium; dripping the gel sealing solution on an axillary bud of the healthy thick branch.
Description
Technical field
A joint position using method for middle concentration IBA and glycerogel, belongs to plant biotechnology field.
Background technology
Cottage propagation is one of vegetative common method of plant, especially trees, and the method has that cost is low, instant effect, cycle short, used branch abundance and can keep the plurality of advantages such as parent's merit.Usually can use dissimilar hormone during cottage propagation, as IBA, IBA, NAA, 2,4-D, GA
3, 6-BA, KT, TDZ, TIBA, ethrel, B
9, PP333, MH etc., the interpolation of above-mentioned hormone can enable plant at body or in vitro ahead of time or postpone to take root, bloom, bear fruit, and increases radical, induction axillary bud sprouting, improves percentage of fertile fruit etc., agricultural production plays important purposes.But the branch being difficult to break up in in-vitro propagate or rooting process, due to the loss of long moisture or nutriment, extremely easily causes death.And the method that existing employing urges root to lure bud to bloom possesses skills the shortcomings such as step is more, cost is high, unstable, in production, effect is unsatisfactory.Therefore, need badly a kind of fast and convenient method for plant axillary bud deriving, take root and Cheng Hua etc.
Summary of the invention
A joint position using method for middle concentration IBA and glycerogel, is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel, cuttage, the dropping of axillalry bud place in described method, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g IBA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the IBA mother liquor of 1mg/mL;
2. working solution preparation: it is concentration hormone working solution in 100mg/L that above-mentioned mother liquor is diluted to concentration according to the ratio of volume ratio 1:10, adds 10ml glycerine according to 90ml hormone working solution simultaneously;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 100mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;
cuttage: branch is inserted in cultivation matrix;
axillalry bud place drips: drip gel confining liquid at the axillalry bud place of branch.
The invention has the advantages that from the viewpoint of water conservation, short root, lure bud, bloom, bear fruit, with hormone working solution for solvent, sodium carboxymethylcellulose and sodium alginate are gel carrier, are mixed with containing gel solution that is dissimilar, concentration hormone.Features such as during use, branch, flower being soaked in slightly above-mentioned solution or above-mentioned solution is dripped at axillalry bud place, film can be formed rapidly after gel drying, reaching the evaporation of minimizing moisture, being continuously applied hormone, be easy and simple to handle.
embodiment:
Below in conjunction with specific embodiment, the present invention is conducted further description.
Embodiment
A joint position using method for middle concentration IBA and glycerogel, is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel, cuttage, the dropping of axillalry bud place in described method, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g IBA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the IBA mother liquor of 1mg/mL;
2. working solution preparation: it is concentration hormone working solution in 100mg/L that above-mentioned mother liquor is diluted to concentration according to the ratio of volume ratio 1:10, adds 10ml glycerine according to 90ml hormone working solution simultaneously;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 100mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;
cuttage: pot marigold branch is inserted in cultivation matrix;
axillalry bud place drips: drip gel confining liquid at the axillalry bud place of branch, after 20-30 days, axillary bud sprouting rate reaches more than 90%, and branch rooting rate reaches more than 80%.
Claims (1)
1. in, a joint position using method for concentration IBA and glycerogel, is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel, cuttage, the dropping of axillalry bud place in described method, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g IBA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the IBA mother liquor of 1mg/mL;
2. working solution preparation: it is concentration hormone working solution in 100mg/L that above-mentioned mother liquor is diluted to concentration according to the ratio of volume ratio 1:10, adds 10ml glycerine according to 90ml hormone working solution simultaneously;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 100mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;
cuttage: branch is inserted in cultivation matrix;
axillalry bud place drips: drip gel confining liquid at the axillalry bud place of branch.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510525515.8A CN105010320A (en) | 2015-08-25 | 2015-08-25 | Node position use method of middle-concentration IBA and glycerol gel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510525515.8A CN105010320A (en) | 2015-08-25 | 2015-08-25 | Node position use method of middle-concentration IBA and glycerol gel |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105010320A true CN105010320A (en) | 2015-11-04 |
Family
ID=54400978
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510525515.8A Pending CN105010320A (en) | 2015-08-25 | 2015-08-25 | Node position use method of middle-concentration IBA and glycerol gel |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105010320A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105519339A (en) * | 2016-01-08 | 2016-04-27 | 何晴雪 | Node order use method for medium-concentration NAA and glycerin gel |
-
2015
- 2015-08-25 CN CN201510525515.8A patent/CN105010320A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| 狄海霞 等: "月季扦插繁育技术研究", 《科技信息》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105519339A (en) * | 2016-01-08 | 2016-04-27 | 何晴雪 | Node order use method for medium-concentration NAA and glycerin gel |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105191919A (en) | Low-concentration NAA and glycerogel root-dipping method | |
| CN105103884A (en) | Low-concentration NAA and glycerin jelly node order using method | |
| CN105010320A (en) | Node position use method of middle-concentration IBA and glycerol gel | |
| CN105123688A (en) | Node order using method of high-concentration KT and glycerine gel | |
| CN105211058A (en) | The joint position using method of a kind of middle concentration IAA and glycerogel | |
| CN105123684A (en) | Node order using method of high-concentration BA and glycerine gel | |
| CN105052906A (en) | Node using method of high-concentration IBA and glycerin gel | |
| CN105165813A (en) | Node order application method of high concentration IAA and glycerin gel | |
| CN105191926A (en) | Section using method of middle-concentration BA and glycerin gel | |
| CN105191929A (en) | Section-position using method adopting high-concentration 2,4-D and glycerinum gel | |
| CN105191920A (en) | Section-position using method adopting high-concentration NAA gel | |
| CN104996412A (en) | Section position use method of medium-concentration KT and glycerogel | |
| CN105123674A (en) | Section position using method of low concentration NAA gel | |
| CN105191921A (en) | Root dipping method adopting high-concentration NAA gel | |
| CN105191917A (en) | Node position use method of low-concentration IAA and glycerogel | |
| CN105191928A (en) | Node position use method of low-concentration 2,4-D and glycerogel | |
| CN105519339A (en) | Node order use method for medium-concentration NAA and glycerin gel | |
| CN105052907A (en) | Node position using method for low-concentration IBA and glycerin gel | |
| CN105104368A (en) | Node application method of medium-concentration IBA gel | |
| CN105052914A (en) | Node position using method for low-concentration KT and glycerin gel | |
| CN105123676A (en) | Node order using method of medium-concentration IAA gel | |
| CN105191927A (en) | Section using method of middle-concentration 2,4-D and glycerin gel | |
| CN105519340A (en) | Node order use method for high-concentration NAA and glycerin gel | |
| CN105165817A (en) | Node order application method of medium concentration KT gel | |
| CN105103885A (en) | Low-concentration BA and glycerin jelly node order using method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20151104 |
|
| RJ01 | Rejection of invention patent application after publication |