CN105191929A - Section-position using method adopting high-concentration 2,4-D and glycerinum gel - Google Patents
Section-position using method adopting high-concentration 2,4-D and glycerinum gel Download PDFInfo
- Publication number
- CN105191929A CN105191929A CN201510525778.9A CN201510525778A CN105191929A CN 105191929 A CN105191929 A CN 105191929A CN 201510525778 A CN201510525778 A CN 201510525778A CN 105191929 A CN105191929 A CN 105191929A
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- CN
- China
- Prior art keywords
- hormone
- concentration
- working solution
- gel
- mother liquor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 title claims abstract description 15
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 title claims abstract description 15
- 238000000034 method Methods 0.000 title claims abstract description 15
- 239000012224 working solution Substances 0.000 claims abstract description 18
- 239000012452 mother liquor Substances 0.000 claims abstract description 11
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 5
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000001768 carboxy methyl cellulose Substances 0.000 claims abstract description 5
- 239000007788 liquid Substances 0.000 claims abstract description 5
- 239000000661 sodium alginate Substances 0.000 claims abstract description 5
- 235000010413 sodium alginate Nutrition 0.000 claims abstract description 5
- 229940005550 sodium alginate Drugs 0.000 claims abstract description 5
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 claims abstract description 5
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 claims abstract description 5
- 239000005556 hormone Substances 0.000 claims description 23
- 229940088597 hormone Drugs 0.000 claims description 23
- 238000002360 preparation method Methods 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- 235000011187 glycerol Nutrition 0.000 claims description 6
- 229930192334 Auxin Natural products 0.000 claims description 3
- 239000002363 auxin Substances 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims description 3
- 239000011159 matrix material Substances 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 4
- 235000013399 edible fruits Nutrition 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 2
- UDPGUMQDCGORJQ-UHFFFAOYSA-N (2-chloroethyl)phosphonic acid Chemical compound OP(O)(=O)CCCl UDPGUMQDCGORJQ-UHFFFAOYSA-N 0.000 description 1
- ZMZGFLUUZLELNE-UHFFFAOYSA-N 2,3,5-triiodobenzoic acid Chemical compound OC(=O)C1=CC(I)=CC(I)=C1I ZMZGFLUUZLELNE-UHFFFAOYSA-N 0.000 description 1
- 240000001432 Calendula officinalis Species 0.000 description 1
- 235000005881 Calendula officinalis Nutrition 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- KNXVOGGZOFOROK-UHFFFAOYSA-N trimagnesium;dioxido(oxo)silane;hydroxy-oxido-oxosilane Chemical compound [Mg+2].[Mg+2].[Mg+2].O[Si]([O-])=O.O[Si]([O-])=O.[O-][Si]([O-])=O.[O-][Si]([O-])=O KNXVOGGZOFOROK-UHFFFAOYSA-N 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a section-position using method adopting high-concentration 2,4-D and glycerinum gel and belongs to the technical field of plant biology. At first, 2,4-D mother liquor of 1 mg/mL is prepared; the mother liquor is diluted into a high-concentration 2,4-D working solution of 500 mg/L; the working solution is prepared into high-concentration 2,4-D and glycerinum gel confining liquid containing sodium carboxymethyl cellulose with the mass concentration of 1% and sodium alginate with the mass concentration of 8%; a branch is inserted into a culture medium; the gel confining liquid is dripped into an axillary bud of the healthy and strong branch.
Description
Technical field
The joint position using method of a kind of high concentration 2,4-D and glycerogel, belongs to plant biotechnology field.
Background technology
Cottage propagation is one of vegetative common method of plant, especially trees, and the method has that cost is low, instant effect, cycle short, used branch abundance and can keep the plurality of advantages such as parent's merit.Usually can use dissimilar hormone during cottage propagation, as 2,4-D, IBA, NAA, 2,4-D, GA
3, 6-BA, KT, TDZ, TIBA, ethrel, B
9, PP333, MH etc., the interpolation of above-mentioned hormone can enable plant at body or in vitro ahead of time or postpone to take root, bloom, bear fruit, and increases radical, induction axillary bud sprouting, improves percentage of fertile fruit etc., agricultural production plays important purposes.But the branch being difficult to break up in in-vitro propagate or rooting process, due to the loss of long moisture or nutriment, extremely easily causes death.And the method that existing employing urges root to lure bud to bloom possesses skills the shortcomings such as step is more, cost is high, unstable, in production, effect is unsatisfactory.Therefore, need badly a kind of fast and convenient method for plant axillary bud deriving, take root and Cheng Hua etc.
Summary of the invention
The joint position using method of a kind of high concentration 2,4-D and glycerogel, is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel, cuttage, the dropping of axillalry bud place in described method, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g 2,4-D with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be 2, the 4-D mother liquors of 1mg/mL;
2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:2 the High concentration auxin working solution that concentration is 500mg/L, adds 10ml glycerine according to 90ml hormone working solution simultaneously;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 500mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;
cuttage: branch is inserted in cultivation matrix;
axillalry bud place drips: drip gel confining liquid at the axillalry bud place of branch.
The invention has the advantages that from the viewpoint of water conservation, short root, lure bud, bloom, bear fruit, with hormone working solution for solvent, sodium carboxymethylcellulose and sodium alginate are gel carrier, are mixed with containing gel solution that is dissimilar, concentration hormone.Features such as during use, branch, flower being soaked in slightly above-mentioned solution or above-mentioned solution is dripped at axillalry bud place, film can be formed rapidly after gel drying, reaching the evaporation of minimizing moisture, being continuously applied hormone, be easy and simple to handle.
embodiment:
Below in conjunction with specific embodiment, the present invention is conducted further description.
Embodiment
The joint position using method of a kind of high concentration 2,4-D and glycerogel, is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel, cuttage, the dropping of axillalry bud place in described method, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g 2,4-D with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be 2, the 4-D mother liquors of 1mg/mL;
2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:2 the High concentration auxin working solution that concentration is 500mg/L, adds 10ml glycerine according to 90ml hormone working solution simultaneously;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 500mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;
cuttage: pot marigold branch is inserted in cultivation matrix;
axillalry bud place drips: drip gel confining liquid at the axillalry bud place of branch.
Claims (1)
1. the joint position using method of a high concentration 2,4-D and glycerogel, is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel, cuttage, the dropping of axillalry bud place in described method, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g 2,4-D with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be 2, the 4-D mother liquors of 1mg/mL;
2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:2 the High concentration auxin working solution that concentration is 500mg/L, adds 10ml glycerine according to 90ml hormone working solution simultaneously;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 500mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;
cuttage: branch is inserted in cultivation matrix;
axillalry bud place drips: drip gel confining liquid at the axillalry bud place of branch.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510525778.9A CN105191929A (en) | 2015-08-25 | 2015-08-25 | Section-position using method adopting high-concentration 2,4-D and glycerinum gel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510525778.9A CN105191929A (en) | 2015-08-25 | 2015-08-25 | Section-position using method adopting high-concentration 2,4-D and glycerinum gel |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105191929A true CN105191929A (en) | 2015-12-30 |
Family
ID=54940220
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510525778.9A Pending CN105191929A (en) | 2015-08-25 | 2015-08-25 | Section-position using method adopting high-concentration 2,4-D and glycerinum gel |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105191929A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105519340A (en) * | 2016-01-08 | 2016-04-27 | 何晴雪 | Node order use method for high-concentration NAA and glycerin gel |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1061885A (en) * | 1992-01-17 | 1992-06-17 | 北京林业大学 | High efficient growing promoter for root of plant |
| CN1114129A (en) * | 1994-06-16 | 1996-01-03 | 王华芳 | Process for breeding and culturing azalea |
| WO2001084928A1 (en) * | 2000-05-11 | 2001-11-15 | Bayer Cropscience Gmbh | Combinations of plant protection agents with organic or inorganic matrix materials |
| CN102599161A (en) * | 2012-02-09 | 2012-07-25 | 濮阳市林业科学院 | Hormone hydrogel rooting film |
-
2015
- 2015-08-25 CN CN201510525778.9A patent/CN105191929A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1061885A (en) * | 1992-01-17 | 1992-06-17 | 北京林业大学 | High efficient growing promoter for root of plant |
| CN1114129A (en) * | 1994-06-16 | 1996-01-03 | 王华芳 | Process for breeding and culturing azalea |
| WO2001084928A1 (en) * | 2000-05-11 | 2001-11-15 | Bayer Cropscience Gmbh | Combinations of plant protection agents with organic or inorganic matrix materials |
| CN102599161A (en) * | 2012-02-09 | 2012-07-25 | 濮阳市林业科学院 | Hormone hydrogel rooting film |
Non-Patent Citations (1)
| Title |
|---|
| 狄海霞 等: "月季扦插繁育技术研究", 《科技信息》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105519340A (en) * | 2016-01-08 | 2016-04-27 | 何晴雪 | Node order use method for high-concentration NAA and glycerin gel |
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| PB01 | Publication | ||
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20151230 |
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| WD01 | Invention patent application deemed withdrawn after publication |