CN105104370A - Root dipping method using high-concentration BA and glycerin gel - Google Patents

Abstract

The invention discloses a root dipping method using high-concentration BA and glycerin gel and belongs to the technical field of plant biology. The root dipping method comprises the following steps: firstly, preparing a 1mg/mL BA mother solution; diluting the mother solution into a 500mg/L high-concentration BA working solution; preparing a high-concentration BA and glycerin gel blocking solution containing sodium carboxymethyl cellulose with the mass concentration of 1% and sodium alginate with the mass concentration of 8% from the working solution; dipping the lower ends of healthy and sturdy branches into the gel blocking solution for 3-5 seconds and taking out the braches; and inserting the braches into a culture medium to take roots.

Description

A kind of high concentration BA and glycerogel be stained with method for root

Technical field

High concentration BA and glycerogel be stained with a method for root, belong to plant biotechnology field.

Background technology

Cottage propagation is one of vegetative common method of plant, especially trees, and the method has that cost is low, instant effect, cycle short, used branch abundance and can keep the plurality of advantages such as parent's merit.Usually can use dissimilar hormone during cottage propagation, as BA, IBA, NAA, 2,4-D, GA ₃, 6-BA, KT, TDZ, TIBA, ethrel, B ₉, PP333, MH etc., the interpolation of above-mentioned hormone can enable plant at body or in vitro ahead of time or postpone to take root, bloom, bear fruit, and increases radical, induction axillary bud sprouting, improves percentage of fertile fruit etc., agricultural production plays important purposes.But the branch being difficult to break up in in-vitro propagate or rooting process, due to the loss of long moisture or nutriment, extremely easily causes death.And the method that existing employing urges root to lure bud to bloom possesses skills the shortcomings such as step is more, cost is high, unstable, in production, effect is unsatisfactory.Therefore, need badly a kind of fast and convenient method for plant axillary bud deriving, take root and Cheng Hua etc.

Summary of the invention

High concentration BA and glycerogel be stained with a method for root, it is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel in described method, be stained with root, cuttage, concrete operation step is as follows:

1. hormone mother liquor: precise weight is, after the KOH of a little 1M of BA of 0.1g dissolves, be settled to 100mL, be the BA mother liquor of 1mg/mL with distilled water;

2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:2 the High concentration auxin working solution that concentration is 500mg/L, adds 10ml glycerine according to 90ml hormone working solution simultaneously;

3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 500mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;

be stained with root: branch lower end sturdy for health is sticked in 3-5 in above-mentioned gel confining liquid and take out after second;

cuttage: above-mentioned branch is inserted in cultivation matrix and takes root.

The invention has the advantages that from the viewpoint of water conservation, short root, lure bud, bloom, bear fruit, with hormone working solution for solvent, sodium carboxymethylcellulose and sodium alginate are gel carrier, are mixed with containing gel solution that is dissimilar, concentration hormone.Features such as during use, branch, flower being soaked in slightly above-mentioned solution or above-mentioned solution is dripped at axillalry bud place, film can be formed rapidly after gel drying, reaching the evaporation of minimizing moisture, being continuously applied hormone, be easy and simple to handle.

embodiment:

Below in conjunction with specific embodiment, the present invention is conducted further description.

Embodiment

High concentration BA and glycerogel be stained with a method for root, it is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel in described method, be stained with root, cuttage, concrete operation step is as follows:

1. hormone mother liquor: precise weight is, after the KOH of a little 1M of BA of 0.1g dissolves, be settled to 100mL, be the BA mother liquor of 1mg/mL with distilled water;

2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:2 the High concentration auxin working solution that concentration is 500mg/L, simultaneously add 10ml glycerine according to 90ml hormone working solution;

3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 500mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;

be stained with root: 8-10cm is long, healthy sturdy Chinese littleleaf box branch lower end sticks in 3-5 in above-mentioned gel confining liquid and takes out after second;

cuttage: above-mentioned branch is inserted in cultivation matrix and takes root.

Claims (1)

1. high concentration BA and glycerogel be stained with a method for root, it is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel in described method, be stained with root, cuttage, concrete operation step is as follows:

1. hormone mother liquor: precise weight is, after the KOH of a little 1M of BA of 0.1g dissolves, be settled to 100mL, be the BA mother liquor of 1mg/mL with distilled water;

2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:2 the High concentration auxin working solution that concentration is 500mg/L, adds 10ml glycerine according to 90ml hormone working solution simultaneously;

3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 500mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;

be stained with root: branch lower end sturdy for health is sticked in 3-5 in above-mentioned gel confining liquid and take out after second;

cuttage: above-mentioned branch is inserted in cultivation matrix and takes root.