CN105061068B - A kind of Moringa polysaccharide enriched biological liquid fertilizer and preparation method thereof - Google Patents
A kind of Moringa polysaccharide enriched biological liquid fertilizer and preparation method thereof Download PDFInfo
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- CN105061068B CN105061068B CN201510504451.3A CN201510504451A CN105061068B CN 105061068 B CN105061068 B CN 105061068B CN 201510504451 A CN201510504451 A CN 201510504451A CN 105061068 B CN105061068 B CN 105061068B
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Abstract
The invention discloses a kind of Moringa polysaccharide enriched biological liquid fertilizers and preparation method thereof, the fertilizer includes 5 ~ 15 parts of Moringa polysaccharide, 10 ~ 25 parts of γ polyglutamic acids, 3 ~ 8 parts of attapulgite, 3 ~ 8 parts of Moringa nutritious protein liquid, 10 ~ 20 parts of bacillus megaterium liquid, 5 ~ 15 parts of bacillus subtilis bacterium solution, 5 ~ 15 parts of 5 ~ 15 parts of bacillus licheniformis liquid, 5 ~ 15 parts of unwrapping wire bacterium solution, 5 ~ 15 parts of lactobacillus plantarum liquid and photosynthetic bacterium solution；Preparation method is by Moringa polysaccharide, γ polyglutamic acids, the probiotic composition and other Organic nutrients contained in the fertilizer can play apparent synergistic effect, it can promote increasing crop yield 15% and 25% or so respectively compared to independent beneficial bacterium analysis fertilizer and independent Organic nutrient fertilizer, and application method is various, the strong root strong sprout that crop can be rapidly promoted, improves the quality of crops, agricultural product can be promoted to increase production 20% or so compared to common fertilizer.
Description
Technical field
The present invention relates to pharmaceutical technology fields, and in particular to a kind of Moringa polysaccharide enriched biological liquid fertilizer and its preparation side
Method.
Background technology
Since the long-time service of chemical fertilizer can damage the crumb structure of the suitable Bacterial community of soil and soil, so in order to protect
The ecology and soil fertility of soil are protected, the use of microbial manure is more and more extensive.But existing liquid microbe currently on the market
Fertilizer is monistic biological probiotics fertilizer, such as azotobacteria fertilizer, phosphobacteria and B. mucilaginocus, the exclusive use of these fertilizer mostly
It is only capable of meeting the needs of crop is to single nutrition, and of high cost, dosage is big, such as with the use of it is difficult to accomplish to combine reasonable, proportioning
Science, thus the fertilizer efficiency of fertilizer cannot be played well.
A kind of good bio-feritlizer has strict requirements to living bacteria count, miscellaneous bacteria rate, shelf life etc., but
The poor management lattice at present to bio-feritlizer, in addition occur without production licence, production technology is backward, effective bacterial content is low,
The exceeded phenomenon of miscellaneous bacteria, some producers are added to an inappropriate amount of chemical fertilizer or additive to improve the N-P-K content in fertilizer,
Cause effective bacterium dead, loses the effect of bio-fertilizer, loss is brought to agricultural production.
Invention content
To solve the above problems, the object of the present invention is to provide a kind of Moringa polysaccharide enriched biological liquid fertilizer and its preparations
Method.
The present invention to achieve the above object, is achieved through the following technical solutions：
A kind of Moringa polysaccharide enriched biological liquid fertilizer consists of the following compositions in parts by weight：Moringa polysaccharide 5 ~ 15
Part, 10 ~ 25 parts of gamma-polyglutamic acid, 3 ~ 8 parts of attapulgite, 3 ~ 8 parts of Moringa nutritious protein liquid, bacillus megaterium liquid 10 ~ 20
Part, 5 ~ 15 parts of bacillus subtilis bacterium solution, 5 ~ 15 parts of bacillus licheniformis liquid, 5 ~ 15 parts of unwrapping wire bacterium solution, lactobacillus plantarum liquid 5 ~ 15
5 ~ 15 parts of part and photosynthetic bacterium solution；The Moringa nutritious protein liquid is obtained by following steps：Moringa seeds are crushed to 80 ~ 100
Mesh, addition water, which stirs, obtains slurries for 0.5 ~ 1 hour, and the weight ratio of moringa seeds and water is 1: 3 ~ 5, and gained slurries are carried out micropore mistake
Filtrate is collected in filter, and gained filtrate is Moringa nutritious protein liquid.
To further realize the purpose of the present invention, following technical scheme can also be used：
Preferred a kind of Moringa polysaccharide enriched biological liquid fertilizer, in every milliliter of Moringa polysaccharide enriched biological liquid fertilizer
Viable count is 8 ~ 1,200,000,000.
A kind of preferred Moringa polysaccharide enriched biological liquid fertilizer consists of the following compositions in parts by weight：Moringa polysaccharide
10 parts, 20 parts of gamma-polyglutamic acid, 5 parts of attapulgite, 5 parts of Moringa nutritious protein liquid, 15 parts of bacillus megaterium liquid, withered grass bud
10 parts of 10 parts of spore bacillus liquid, 10 parts of bacillus licheniformis liquid, 10 parts of unwrapping wire bacterium solution, 10 parts of lactobacillus plantarum liquid and photosynthetic bacterium solution,
The Moringa nutritious protein liquid is obtained by following steps：Moringa seeds are crushed to 90 mesh, water stirring is added and obtains within 45 minutes
The weight ratio of slurries, moringa seeds and water is 1: 4, and gained slurries are carried out micro porous filtration, collect filtrate, gained filtrate is Moringa
Nutritious protein liquid.
A kind of further preferred Moringa polysaccharide enriched biological liquid fertilizer, every milliliter of Moringa polysaccharide enriched biological Liquid Fertilizer
Viable count in material is 900,000,000.
The invention also includes a kind of preparation methods of Moringa polysaccharide enriched biological liquid fertilizer, include the following steps：
1. the preparation of Moringa nutritious protein liquid：Moringa seeds are crushed to 80 ~ 100 mesh, water stirring is added and obtains within 0.5 ~ 1 hour
The weight ratio of slurries, moringa seeds and water is 1: 3 ~ 5, and gained slurries are carried out micro porous filtration, collect filtrate, gained filtrate is as peppery
The wooden nutritious protein liquid；
2. attapulgite is crushed to 200 mesh or more, attapulgite fine powder is obtained；
3. in parts by weight, by 5 ~ 15 parts of Moringa polysaccharide, 10 ~ 25 parts of gamma-polyglutamic acid, 2. gained attapulgite is thin for step
3 ~ 8 parts of powder, step 1. 3 ~ 8 parts of gained Moringa nutritious protein liquid, 10 ~ 20 parts of bacillus megaterium liquid, bacillus subtilis bacterium solution 5 ~
15 parts, 5 ~ 15 parts of bacillus licheniformis liquid, 5 ~ 15 parts of unwrapping wire bacterium solution, 5 ~ 15 parts of 5 ~ 15 parts of lactobacillus plantarum liquid and photosynthetic bacterium solution exist
It is carried out liquid fermentation at 25 ~ 30 DEG C and obtains within 14 ~ 16 days Moringa polysaccharide enriched biological liquid fertilizer.
A kind of preferred preparation method of Moringa polysaccharide enriched biological liquid fertilizer, includes the following steps：
1. the preparation of Moringa nutritious protein liquid：Moringa seeds are crushed to 90 mesh, water stirring is added and obtains slurries within 45 minutes, it is peppery
The weight ratio of the wooden seed and water is 1: 4, and gained slurries are carried out micro porous filtration, collects filtrate, and gained filtrate is Moringa albumen battalion
Nutrient solution；
2. attapulgite is crushed to 200 mesh or more, attapulgite fine powder is obtained；
3. in parts by weight, by 10 parts of Moringa polysaccharide, 20 parts of gamma-polyglutamic acid, step 2. gained attapulgite fine powder 5
Part, step 1. 5 parts of gained Moringa nutritious protein liquid, 15 parts of bacillus megaterium liquid, 10 parts of bacillus subtilis bacterium solution, lichens gemma
10 parts of bacillus liquid, 10 parts of unwrapping wire bacterium solution, 10 parts of 10 parts of lactobacillus plantarum liquid and photosynthetic bacterium solution carry out liquid fermentation 15 at 28 DEG C
It obtains Moringa polysaccharide enriched biological liquid fertilizer.
The advantage of the invention is that：
The Moringa polysaccharide enriched biological liquid fertilizer formula combination of the present invention is scientific and reasonable, effective viable bacteria in every milliliter of fertilizer
Number can reach 1,000,000,000 or so, and total nutrient amount reaches 13% or so, and miscellaneous bacteria rate is low, the index well below national normal value,
Nutritional ingredient in the liquid fertilizer not only containing inorganic fertilizer and organic fertilizer but also contain compound probiotic, farming can not only be provided
Object all kinds of nutrients needed for growth and development, improve the water holding capacity of soil, the pH-value of balanced soil, and wherein contain
Compound probiotic have the function of apparent dissolving phosphor and dissolving potassium, and a variety of enzymes caused by its mass propagation can reduce in organic matter
With the toxin in soil, the physiological functions such as crop photosynthesis, aerobic respiration, fixed nitrogen, carbon sequestration can be promoted, harmful microorganism is risen
It is acted on to biological control, enhances the resistance of crops；
The probiotic composition contained in the Moringa polysaccharide enriched biological liquid fertilizer of the present invention and other Organic nutrients
Apparent synergistic effect can be played, can promote agriculture respectively compared to independent beneficial bacterium analysis fertilizer and independent Organic nutrient fertilizer
Crop yield 15% and 25% or so；
The application method of the Moringa polysaccharide enriched biological liquid fertilizer of the present invention is various, can use foliage spray, pour root, leaching
The modes such as kind and root dipping directly act on crops, and can generate plant hormone substance in each period of crop stimulates farming
The growth of object, and absorb soon, the strong root strong sprout of crop, flower and fruit protecting can be promoted to promote the hyperchromic sweetened of fruit, improve farming
The quality of object, and have wide range of applications, multiple kinds of crops is adapted to, agricultural product can be promoted to increase production 20% or so compared to common fertilizer.
The preparation method flow of the Moringa polysaccharide enriched biological liquid fertilizer of the present invention is few, easy implementation simple for process, fits
Close large-scale production.
Specific implementation mode
A kind of Moringa polysaccharide enriched biological liquid fertilizer consists of the following compositions in parts by weight：Moringa polysaccharide 5 ~ 15
Part, 10 ~ 25 parts of gamma-polyglutamic acid, 3 ~ 8 parts of attapulgite, 3 ~ 8 parts of Moringa nutritious protein liquid, bacillus megaterium liquid 10 ~ 20
Part, 5 ~ 15 parts of bacillus subtilis bacterium solution, 5 ~ 15 parts of bacillus licheniformis liquid, 5 ~ 15 parts of unwrapping wire bacterium solution, lactobacillus plantarum liquid 5 ~ 15
5 ~ 15 parts of part and photosynthetic bacterium solution；The Moringa nutritious protein liquid is obtained by following steps：Moringa seeds are crushed to 80 ~ 100
Mesh, addition water, which stirs, obtains slurries for 0.5 ~ 1 hour, and the weight ratio of moringa seeds and water is 1: 3 ~ 5, and gained slurries are carried out micropore mistake
Filtrate is collected in filter, and gained filtrate is Moringa nutritious protein liquid.
To further realize the purpose of the present invention, following technical scheme can also be used：
Preferred a kind of Moringa polysaccharide enriched biological liquid fertilizer, in every milliliter of Moringa polysaccharide enriched biological liquid fertilizer
Viable count is 8 ~ 1,200,000,000.
A kind of preferred Moringa polysaccharide enriched biological liquid fertilizer consists of the following compositions in parts by weight：Moringa polysaccharide
10 parts, 20 parts of gamma-polyglutamic acid, 5 parts of attapulgite, 5 parts of Moringa nutritious protein liquid, 15 parts of bacillus megaterium liquid, withered grass bud
10 parts of 10 parts of spore bacillus liquid, 10 parts of bacillus licheniformis liquid, 10 parts of unwrapping wire bacterium solution, 10 parts of lactobacillus plantarum liquid and photosynthetic bacterium solution,
The Moringa nutritious protein liquid is obtained by following steps：Moringa seeds are crushed to 90 mesh, water stirring is added and obtains within 45 minutes
The weight ratio of slurries, moringa seeds and water is 1: 4, and gained slurries are carried out micro porous filtration, collect filtrate, gained filtrate is Moringa
Nutritious protein liquid.
A kind of further preferred Moringa polysaccharide enriched biological liquid fertilizer, every milliliter of Moringa polysaccharide enriched biological Liquid Fertilizer
Viable count in material is 900,000,000.
The invention also includes a kind of preparation methods of Moringa polysaccharide enriched biological liquid fertilizer, include the following steps：
1. the preparation of Moringa nutritious protein liquid：Moringa seeds are crushed to 80 ~ 100 mesh, water stirring is added and obtains within 0.5 ~ 1 hour
The weight ratio of slurries, moringa seeds and water is 1: 3 ~ 5, and gained slurries are carried out micro porous filtration, collect filtrate, gained filtrate is as peppery
The wooden nutritious protein liquid；
2. attapulgite is crushed to 200 mesh or more, attapulgite fine powder is obtained；
3. in parts by weight, by 5 ~ 15 parts of Moringa polysaccharide, 10 ~ 25 parts of gamma-polyglutamic acid, 2. gained attapulgite is thin for step
3 ~ 8 parts of powder, step 1. 3 ~ 8 parts of gained Moringa nutritious protein liquid, 10 ~ 20 parts of bacillus megaterium liquid, bacillus subtilis bacterium solution 5 ~
15 parts, 5 ~ 15 parts of bacillus licheniformis liquid, 5 ~ 15 parts of unwrapping wire bacterium solution, 5 ~ 15 parts of 5 ~ 15 parts of lactobacillus plantarum liquid and photosynthetic bacterium solution exist
It is carried out liquid fermentation at 25 ~ 30 DEG C and obtains within 14 ~ 16 days Moringa polysaccharide enriched biological liquid fertilizer.
A kind of preferred preparation method of Moringa polysaccharide enriched biological liquid fertilizer, includes the following steps：
1. the preparation of Moringa nutritious protein liquid：Moringa seeds are crushed to 90 mesh, water stirring is added and obtains slurries within 45 minutes, it is peppery
The weight ratio of the wooden seed and water is 1: 4, and gained slurries are carried out micro porous filtration, collects filtrate, and gained filtrate is Moringa albumen battalion
Nutrient solution；
2. attapulgite is crushed to 200 mesh or more, attapulgite fine powder is obtained；
3. in parts by weight, by 10 parts of Moringa polysaccharide, 20 parts of gamma-polyglutamic acid, step 2. gained attapulgite fine powder 5
Part, step 1. 5 parts of gained Moringa nutritious protein liquid, 15 parts of bacillus megaterium liquid, 10 parts of bacillus subtilis bacterium solution, lichens gemma
10 parts of bacillus liquid, 10 parts of unwrapping wire bacterium solution, 10 parts of 10 parts of lactobacillus plantarum liquid and photosynthetic bacterium solution carry out liquid fermentation 15 at 28 DEG C
It obtains Moringa polysaccharide enriched biological liquid fertilizer.
The Moringa polysaccharide of the present invention is to obtain leaf of Moringa powder by leaf of Moringa crushed after being dried to 100 mesh or so, and gained is peppery
The wooden leaf powder is mixed with water according to weight ratio 1: 3 ~ 5, obtains mixed liquor, and mixed liquor is flowed back 100 ~ 120 points at 80 ~ 90 DEG C of temperature
It is filtered after clock, filtrate obtains Moringa polysaccharide through vacuum distillation.
Bacillus megaterium liquid, bacillus subtilis bacterium solution, bacillus licheniformis liquid, unwrapping wire bacterium solution, plant used in the present invention
Object lactobacillus liquid and photosynthetic bacterium solution are obtained according to the method culture of known technology.
Embodiment 1
A kind of Moringa polysaccharide enriched biological liquid fertilizer consists of the following compositions in parts by weight：5 parts of Moringa polysaccharide,
10 parts of gamma-polyglutamic acid, 3 parts of attapulgite, 3 parts of Moringa nutritious protein liquid, 10 parts of bacillus megaterium liquid, bacillus subtilis
5 parts of 5 parts of liquid, 5 parts of bacillus licheniformis liquid, 5 parts of unwrapping wire bacterium solution, 5 parts of lactobacillus plantarum liquid and photosynthetic bacterium solution；The Moringa egg
White nutrient solution is obtained by following steps：Moringa seeds are crushed to 80 mesh, water stirring is added and obtains slurries, moringa seeds within 0.5 hour
Weight ratio with water is 1: 3, and gained slurries are carried out micro porous filtration, collect filtrate, gained filtrate is Moringa nutritious protein liquid.
Embodiment 2
A kind of Moringa polysaccharide enriched biological liquid fertilizer consists of the following compositions in parts by weight：15 parts of Moringa polysaccharide,
25 parts of gamma-polyglutamic acid, 8 parts of attapulgite, 8 parts of Moringa nutritious protein liquid, 20 parts of bacillus megaterium liquid, bacillus subtilis
15 parts of 15 parts of liquid, 15 parts of bacillus licheniformis liquid, 15 parts of unwrapping wire bacterium solution, 15 parts of lactobacillus plantarum liquid and photosynthetic bacterium solution；Described
Moringa nutritious protein liquid is obtained by following steps：Moringa seeds are crushed to 100 mesh, water stirring is added and obtains slurries within 1 hour, it is peppery
The weight ratio of the wooden seed and water is 1: 5, and gained slurries are carried out micro porous filtration, collects filtrate, and gained filtrate is Moringa albumen battalion
Nutrient solution.
Embodiment 3
A kind of Moringa polysaccharide enriched biological liquid fertilizer consists of the following compositions in parts by weight：12 parts of Moringa polysaccharide,
18 parts of gamma-polyglutamic acid, 7 parts of attapulgite, 4 parts of Moringa nutritious protein liquid, 12 parts of bacillus megaterium liquid, bacillus subtilis
7 parts of 8 parts of liquid, 12 parts of bacillus licheniformis liquid, 9 parts of unwrapping wire bacterium solution, 11 parts of lactobacillus plantarum liquid and photosynthetic bacterium solution；The Moringa
Nutritious protein liquid is obtained by following steps：Moringa seeds are crushed to 90 mesh, water stirring is added and obtains slurries, moringa seeds within 45 minutes
Weight ratio with water is 1: 3.5, and gained slurries are carried out micro porous filtration, collect filtrate, gained filtrate is Moringa nutrient protein
Liquid.
Embodiment 4
A kind of Moringa polysaccharide enriched biological liquid fertilizer consists of the following compositions in parts by weight：8 parts of Moringa polysaccharide,
25 parts of gamma-polyglutamic acid, 3 parts of attapulgite, 4 parts of Moringa nutritious protein liquid, 14 parts of bacillus megaterium liquid, bacillus subtilis
10 parts of 6 parts of liquid, 8 parts of bacillus licheniformis liquid, 12 parts of unwrapping wire bacterium solution, 13 parts of lactobacillus plantarum liquid and photosynthetic bacterium solution；Described is peppery
The wooden nutritious protein liquid is obtained by following steps：Moringa seeds are crushed to 85 mesh, water stirring is added and obtains slurries, Moringa within 50 minutes
The weight ratio of seed and water is 1: 4.5, and gained slurries are carried out micro porous filtration, collects filtrate, and gained filtrate is Moringa albumen battalion
Nutrient solution.
Embodiment 5
A kind of Moringa polysaccharide enriched biological liquid fertilizer consists of the following compositions in parts by weight：10 parts of Moringa polysaccharide,
20 parts of gamma-polyglutamic acid, 5 parts of attapulgite, 5 parts of Moringa nutritious protein liquid, 15 parts of bacillus megaterium liquid, bacillus subtilis
10 parts of 10 parts of liquid, 10 parts of bacillus licheniformis liquid, 10 parts of unwrapping wire bacterium solution, 10 parts of lactobacillus plantarum liquid and photosynthetic bacterium solution, it is described
Moringa nutritious protein liquid is obtained by following steps：Moringa seeds are crushed to 90 mesh, water stirring is added and obtains slurries within 45 minutes, it is peppery
The weight ratio of the wooden seed and water is 1: 4, and gained slurries are carried out micro porous filtration, collects filtrate, and gained filtrate is Moringa albumen battalion
Nutrient solution.
Embodiment 6
A kind of preparation method of Moringa polysaccharide enriched biological liquid fertilizer obtained by quality proportioning by embodiment 1, including
Following steps：
1. the preparation of Moringa nutritious protein liquid：Moringa seeds are crushed to 80 mesh, water stirring is added and obtains slurries within 0.5 hour,
The weight ratio of moringa seeds and water is 1: 3, and gained slurries are carried out micro porous filtration, collect filtrate, gained filtrate is Moringa albumen
Nutrient solution.
2. attapulgite is crushed to 200 mesh or more, attapulgite fine powder is obtained；
3. in parts by weight, by 5 parts of Moringa polysaccharide, 10 parts of gamma-polyglutamic acid, step 2. gained attapulgite fine powder 3
Part, step 1. 3 parts of gained Moringa nutritious protein liquid, 10 parts of bacillus megaterium liquid, 5 parts of bacillus subtilis bacterium solution, lichens gemma
5 parts of 5 parts of bacillus liquid, 5 parts of unwrapping wire bacterium solution, 5 parts of lactobacillus plantarum liquid and photosynthetic bacterium solution carry out liquid fermentation 14 days at 25 DEG C
To Moringa polysaccharide enriched biological liquid fertilizer.
Embodiment 7
A kind of preparation method of Moringa polysaccharide enriched biological liquid fertilizer obtained by quality proportioning by embodiment 2, including
Following steps：
1. the preparation of Moringa nutritious protein liquid：Moringa seeds are crushed to 100 mesh, water stirring is added and obtains slurries within 1 hour, it is peppery
The weight ratio of the wooden seed and water is 1: 5, and gained slurries are carried out micro porous filtration, collects filtrate, and gained filtrate is Moringa albumen battalion
Nutrient solution；
2. attapulgite is crushed to 200 mesh or more, attapulgite fine powder is obtained；
3. in parts by weight, by 15 parts of Moringa polysaccharide, 25 parts of gamma-polyglutamic acid, step 2. gained attapulgite fine powder 8
Part, step 1. 8 parts of gained Moringa nutritious protein liquid, 20 parts of bacillus megaterium liquid, 15 parts of bacillus subtilis bacterium solution, lichens gemma
15 parts of bacillus liquid, 15 parts of unwrapping wire bacterium solution, 15 parts of 15 parts of lactobacillus plantarum liquid and photosynthetic bacterium solution carry out liquid fermentation 16 at 30 DEG C
It obtains Moringa polysaccharide enriched biological liquid fertilizer.
Embodiment 8
A kind of preparation method of Moringa polysaccharide enriched biological liquid fertilizer obtained by quality proportioning by embodiment 3, including
Following steps：
1. the preparation of Moringa nutritious protein liquid：Moringa seeds are crushed to 90 mesh, water stirring is added and obtains slurries within 45 minutes, it is peppery
The weight ratio of the wooden seed and water is 1: 3.5, and gained slurries are carried out micro porous filtration, collect filtrate, gained filtrate is Moringa albumen
Nutrient solution；
2. attapulgite is crushed to 200 mesh or more, attapulgite fine powder is obtained；
3. in parts by weight, by 12 parts of Moringa polysaccharide, 18 parts of gamma-polyglutamic acid, step 2. gained attapulgite fine powder 7
Part, step 1. 4 parts of gained Moringa nutritious protein liquid, 12 parts of bacillus megaterium liquid, 8 parts of bacillus subtilis bacterium solution, lichens gemma
12 parts of bacillus liquid, 9 parts of unwrapping wire bacterium solution, 7 parts of 11 parts of lactobacillus plantarum liquid and photosynthetic bacterium solution carry out liquid fermentation 15 days at 28 DEG C
Obtain Moringa polysaccharide enriched biological liquid fertilizer.
Embodiment 9
A kind of preparation method of Moringa polysaccharide enriched biological liquid fertilizer obtained by quality proportioning by embodiment 4, including
Following steps：
1. the preparation of Moringa nutritious protein liquid：Moringa seeds are crushed to 85 mesh, water stirring is added and obtains slurries within 50 minutes, it is peppery
The weight ratio of the wooden seed and water is 1: 4.5, and gained slurries are carried out micro porous filtration, collect filtrate, gained filtrate is Moringa albumen
Nutrient solution；
2. attapulgite is crushed to 200 mesh or more, attapulgite fine powder is obtained；
3. in parts by weight, by 8 parts of Moringa polysaccharide, 25 parts of gamma-polyglutamic acid, step 2. gained attapulgite fine powder 3
Part, step 1. 4 parts of gained Moringa nutritious protein liquid, 14 parts of bacillus megaterium liquid, 16 parts of bacillus subtilis bacterium solution, lichens gemma
8 parts of bacillus liquid, 12 parts of unwrapping wire bacterium solution, 10 parts of 13 parts of lactobacillus plantarum liquid and photosynthetic bacterium solution carry out liquid fermentation 14 days at 26 DEG C
Obtain Moringa polysaccharide enriched biological liquid fertilizer.
Embodiment 10
A kind of preparation method of Moringa polysaccharide enriched biological liquid fertilizer obtained by quality proportioning by embodiment 5, including
Following steps：
1. the preparation of Moringa nutritious protein liquid：Moringa seeds are crushed to 90 mesh, water stirring is added and obtains slurries within 45 minutes, it is peppery
The weight ratio of the wooden seed and water is 1: 4, and gained slurries are carried out micro porous filtration, collects filtrate, and gained filtrate is Moringa albumen battalion
Nutrient solution；
2. attapulgite is crushed to 200 mesh or more, attapulgite fine powder is obtained；
3. in parts by weight, by 10 parts of Moringa polysaccharide, 20 parts of gamma-polyglutamic acid, step 2. gained attapulgite fine powder 5
Part, step 1. 5 parts of gained Moringa nutritious protein liquid, 15 parts of bacillus megaterium liquid, 10 parts of bacillus subtilis bacterium solution, lichens gemma
10 parts of bacillus liquid, 10 parts of unwrapping wire bacterium solution, 10 parts of 10 parts of lactobacillus plantarum liquid and photosynthetic bacterium solution carry out liquid fermentation 15 at 28 DEG C
It obtains Moringa polysaccharide enriched biological liquid fertilizer.
Comparative example 1
In parts by weight, by 12 parts of bacillus megaterium liquid, 8 parts of bacillus subtilis bacterium solution, 12 parts of bacillus licheniformis liquid,
9 parts of unwrapping wire bacterium solution, 7 parts of mixing of 11 parts of lactobacillus plantarum liquid and photosynthetic bacterium solution, obtains microbial liquid fertilizer.
Comparative example 2
In parts by weight, 12 parts of Moringa polysaccharide, 18 parts of gamma-polyglutamic acid, 7 parts of attapulgite and Moringa nutritious protein liquid 4
Part uniformly mixing, is crushed to 200 mesh or more, obtains Moringa polysaccharide fertilizer, the Moringa nutritious protein liquid passes through following steps
It obtains：Moringa seeds are crushed to 90 mesh, addition water, which stirs, obtains slurries for 45 minutes, and the weight ratio of moringa seeds and water is 1: 3.5, will
Gained slurries carry out micro porous filtration, collect filtrate, gained filtrate is Moringa nutritious protein liquid.
The Moringa polysaccharide enriched biological liquid fertilizer of embodiment 1 ~ 5 is detected, as a result as shown in Table 1 and Table 2.
The product technology detection data of the Moringa polysaccharide enriched biological liquid fertilizer of 1 embodiment 1 ~ 5 of table
| Living bacteria count, hundred million/ml | Total nutrient, N+P2O5+KO2% | Miscellaneous bacteria rate, % | pH | The term of validity | |
| Embodiment 1 | 8 | 10 | ≤2 | 6.0 | At least a year |
| Embodiment 2 | 9 | 11 | ≤2 | 7.0 | At least a year |
| Embodiment 3 | 11 | 13 | ≤2 | 6.0 | At least a year |
| Embodiment 4 | 11 | 12 | ≤2 | 7.0 | At least a year |
| Embodiment 5 | 12 | 15 | ≤1 | 7.0 | At least a year |
The innoxious detection data of the product of the Moringa polysaccharide enriched biological liquid fertilizer of 2 embodiment 1 ~ 5 of table
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | |
| Excrement colibacillus group number, a/ml | 5 | 6 | 5 | 5 | 3 |
| Induced worm egg death rate, % | 100 | 100 | 100 | 100 | 100 |
| Arsenic and its compound (in terms of As), mg/kg | It is not detected | It is not detected | It is not detected | It is not detected | It is not detected |
| Cadmium and its compound (in terms of Cd), mg/kg | It is not detected | It is not detected | It is not detected | It is not detected | It is not detected |
| Lead and its compound (in terms of Pb), mg/kg | It is not detected | It is not detected | It is not detected | It is not detected | It is not detected |
| Chromium and compound (in terms of Cr), mg/kg | 10 | 10 | 10 | 10 | 10 |
| Mercury and mercuric compounds (in terms of Hg), mg/kg | It is not detected | It is not detected | It is not detected | It is not detected | It is not detected |
The application method and applicable crops of this product
1. this product is suitable for all kinds of rhizome vegetables, Green leaf vegetable, melon and fruit, flowers, fruit tree and various cereal crops, such as
Rice, corn, wheat etc..
2. dosage
1. foliage spray：Crop puberty, bloom or the phase of bearing fruit sprays Plant Leafs according to 1: 450 ~ 550 times of dilutions
Anyway the face in face, it is primary every sprinkling in 15 days；
2. pouring root：According to pouring root is carried out to crop after 1: 900 ~ 1100 dilutions, the strong robust seedling of root of the crop can be made, and then improve and make
The yield of object；
3. soaking seed：According to soaking seed 8 ~ 12 hours after 1: 250 ~ 350 dilutions, germination percentage and the emergence of crop can be significantly improved
Rate so that healthy seedlings；
4. root dipping：The nursery stock transplanted will be needed, sizes mixing root dipping according to 1: 450 ~ 550 dilution refinement soil, slow seedling can be shifted to an earlier date,
Improve survival rate.
3. using effect
40 mu of plant spring wheat in experimental plot are randomly divided into 8 groups, and every group 5 mu, experimental group 1 ~ 5 uses by the following method
The Moringa polysaccharide enriched biological liquid fertilizer of 1 ~ 5 gained of embodiment；Control group 1 uses 1 gained of comparative example in the same manner
Microbial liquid fertilizer；Control group 2 mainly applies the Moringa polysaccharide fertilizer of 2 gained of comparative example in sedimentation value during grain filling stage of spring wheat, per acre
Usage amount is 50 jin, and control group 3 applies common fertilizer to specifications.
The application method of Moringa polysaccharide enriched biological liquid fertilizer and microbial liquid fertilizer to spring wheat：According to 1: 300
It is sowed after being impregnated 10 hours to seed after dilution, and plant blade face is sprayed according to 1: 500 times of dilution in the pustulation period of spring wheat
Face anyway, primary every sprinkling in 15 days, sprinkling is twice altogether.
Continue maize planting, Moringa polysaccharide enriched biological liquid fertilizer and microbial liquid fertilizer in the plot after getting wheat
Expect the application method to corn：It is sowed after being impregnated 10 hours to seed after being diluted according to 1: 320, and in the tri-leaf period of corn and fringe
The face anyway that phase respectively sprays plant blade face according to 1: 500 times of dilution is primary.
Application method of the Moringa polysaccharide fertilizer in milpa：Control group 2 mainly applies 2 institute of comparative example in the tri-leaf period of corn
The Moringa polysaccharide fertilizer obtained, usage amount is 50 jin per acre.
The yield of wheat and corn is as shown in table 3.
Wheat, the corn yield table of 3 experimental group 1 ~ 5 of table and control group 1 ~ 2
| Wheat per mu yield/kg | Corn per mu yield/kg | |
| Experimental group 1 | 552 | 685 |
| Experimental group 2 | 554 | 690 |
| Experimental group 3 | 562 | 694 |
| Experimental group 4 | 565 | 698 |
| Experimental group 5 | 580 | 720 |
| Control group 1 | 480 | 600 |
| Control group 2 | 450 | 550 |
| Control group 3 | 475 | 590 |
As can be seen from Table 3, using the experimental group 1 ~ 5 of the Moringa polysaccharide enriched biological liquid fertilizer of the present invention compared to application
The per mu yield of 1 wheat of control group and corn of microbial liquid fertilizer increases separately 17.2% and 16.2%, using the Moringa of the present invention
The experimental group 1 ~ 5 of polysaccharide enriched biological liquid fertilizer compares the per mu yield of control group 2 wheat and corn using Moringa polysaccharide fertilizer
25.0% and 26.8% are increased separately, application is compared using the experimental group 1 ~ 5 of the Moringa polysaccharide enriched biological liquid fertilizer of the present invention
The per mu yield of 3 wheat of control group and corn of common fertilizer increases separately 18.4% and 18.2%, it can be seen that, Moringa of the invention is more
Sugared enriched biological liquid fertilizer compares common fertilizer effect of increasing production probiotic composition that is more obvious, and containing in the fertilizer
Apparent synergistic effect can be played with other nutritional ingredients.
Claims (5)
1. a kind of Moringa polysaccharide enriched biological liquid fertilizer, it is characterised in that：In parts by weight, it consists of the following compositions：Moringa
5 ~ 15 parts of polysaccharide, 10 ~ 25 parts of gamma-polyglutamic acid, 3 ~ 8 parts of attapulgite, 3 ~ 8 parts of Moringa nutritious protein liquid, bacillus megaterium
10 ~ 20 parts of liquid, 5 ~ 15 parts of bacillus subtilis bacterium solution, 5 ~ 15 parts of bacillus licheniformis liquid, 5 ~ 15 parts of unwrapping wire bacterium solution, lactobacillus plantarum
5 ~ 15 parts of 5 ~ 15 parts of liquid and photosynthetic bacterium solution；Viable count in every milliliter of Moringa polysaccharide enriched biological liquid fertilizer is 8 ~ 1,200,000,000；Institute
The Moringa nutritious protein liquid stated is obtained by following steps：Moringa seeds are crushed to 80 ~ 100 mesh, water is added and stirs 0.5 ~ 1 hour
Obtain slurries, the weight ratio of moringa seeds and water is 1: 3 ~ 5, and gained slurries are carried out micro porous filtration, collect filtrate, gained filtrate is
For Moringa nutritious protein liquid.
2. a kind of Moringa polysaccharide enriched biological liquid fertilizer according to claim 1, it is characterised in that：In parts by weight,
It consists of the following compositions：10 parts of Moringa polysaccharide, 20 parts of gamma-polyglutamic acid, 5 parts of attapulgite, 5 parts of Moringa nutritious protein liquid are huge
15 parts of Bacterium anthracoides liquid, 10 parts of bacillus subtilis bacterium solution, 10 parts of bacillus licheniformis liquid, 10 parts of unwrapping wire bacterium solution, plant breast bar
10 parts of 10 parts of bacterium solution and photosynthetic bacterium solution, the Moringa nutritious protein liquid are obtained by following steps：Moringa seeds are crushed to 90
Mesh, addition water, which stirs, obtains slurries for 45 minutes, and the weight ratio of moringa seeds and water is 1: 4, and gained slurries are carried out micro porous filtration, are received
Collect filtrate, gained filtrate is Moringa nutritious protein liquid.
3. a kind of Moringa polysaccharide enriched biological liquid fertilizer according to claim 1, it is characterised in that：Every milliliter of Moringa is more
Viable count in sugared enriched biological liquid fertilizer is 900,000,000.
4. a kind of preparation method of Moringa polysaccharide enriched biological liquid fertilizer as described in claim 1, it is characterised in that：Including
Following steps：
1. the preparation of Moringa nutritious protein liquid：Moringa seeds are crushed to 80 ~ 100 mesh, water stirring is added and is starched within 0.5 ~ 1 hour
The weight ratio of liquid, moringa seeds and water is 1: 3 ~ 5, and gained slurries are carried out micro porous filtration, collect filtrate, gained filtrate is Moringa
Nutritious protein liquid；
2. attapulgite is crushed to 200 mesh or more, attapulgite fine powder is obtained；
3. in parts by weight, by 5 ~ 15 parts of Moringa polysaccharide, 10 ~ 25 parts of gamma-polyglutamic acid, step 2. gained attapulgite fine powder 3 ~
8 parts, step 1. 3 ~ 8 parts of gained Moringa nutritious protein liquid, 10 ~ 20 parts of bacillus megaterium liquid, 5 ~ 15 parts of bacillus subtilis bacterium solution,
5 ~ 15 parts of bacillus licheniformis liquid, 5 ~ 15 parts of unwrapping wire bacterium solution, 5 ~ 15 parts of 5 ~ 15 parts of lactobacillus plantarum liquid and photosynthetic bacterium solution are 25 ~ 30
It is carried out liquid fermentation at DEG C and obtains within 14 ~ 16 days Moringa polysaccharide enriched biological liquid fertilizer.
5. a kind of preparation method of Moringa polysaccharide enriched biological liquid fertilizer as claimed in claim 2, it is characterised in that：Including
Following steps：
1. the preparation of Moringa nutritious protein liquid：Moringa seeds are crushed to 90 mesh, water stirring is added and obtains slurries, moringa seeds within 45 minutes
Weight ratio with water is 1: 4, and gained slurries are carried out micro porous filtration, collect filtrate, gained filtrate is Moringa nutritious protein liquid；
2. attapulgite is crushed to 200 mesh or more, attapulgite fine powder is obtained；
3. in parts by weight, by 10 parts of Moringa polysaccharide, 20 parts of gamma-polyglutamic acid, step 2. 5 parts of gained attapulgite fine powder, step
Rapid 5 parts of 1. gained Moringa nutritious protein liquid, 15 parts of bacillus megaterium liquid, 10 parts of bacillus subtilis bacterium solution, bacillus licheniformis
10 parts of 10 parts of liquid, 10 parts of unwrapping wire bacterium solution, 10 parts of lactobacillus plantarum liquid and photosynthetic bacterium solution carry out liquid fermentation 15 days at 28 DEG C
To Moringa polysaccharide enriched biological liquid fertilizer.
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| CN109134047A (en) * | 2018-09-26 | 2019-01-04 | 广西大青山控股集团股份有限公司 | Organic ferment fertilizer and preparation method thereof |
| CN115643963A (en) * | 2022-11-02 | 2023-01-31 | 云南省热带作物科学研究所 | A method for promoting the growth of Moringa oleifera by using composite growth-promoting bacterial strains |
| CN118005464A (en) * | 2024-04-08 | 2024-05-10 | 中建易通科技股份有限公司 | A biocarbon-based remedial soil conditioner and preparation method thereof |
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