CN104971400A - Method for long-term intracerebral pump injection of drugs in great quantity used for primate - Google Patents
Method for long-term intracerebral pump injection of drugs in great quantity used for primate Download PDFInfo
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- CN104971400A CN104971400A CN201510414409.2A CN201510414409A CN104971400A CN 104971400 A CN104971400 A CN 104971400A CN 201510414409 A CN201510414409 A CN 201510414409A CN 104971400 A CN104971400 A CN 104971400A
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- Prior art keywords
- injection
- silica gel
- intracerebral
- needle
- long
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- 238000002347 injection Methods 0.000 title claims abstract description 74
- 239000007924 injection Substances 0.000 title claims abstract description 74
- 239000003814 drug Substances 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 title claims abstract description 23
- 229940079593 drug Drugs 0.000 title claims abstract description 18
- 230000007774 longterm Effects 0.000 title claims abstract description 17
- 241000288906 Primates Species 0.000 title claims abstract description 10
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 31
- 239000000741 silica gel Substances 0.000 claims abstract description 31
- 229910002027 silica gel Inorganic materials 0.000 claims abstract description 31
- 210000004556 brain Anatomy 0.000 claims abstract description 15
- 210000003625 skull Anatomy 0.000 claims abstract description 14
- 241001465754 Metazoa Species 0.000 claims abstract description 10
- 239000002184 metal Substances 0.000 claims abstract description 8
- 210000004761 scalp Anatomy 0.000 claims abstract description 5
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 4
- 238000002695 general anesthesia Methods 0.000 claims abstract description 4
- 239000000243 solution Substances 0.000 claims abstract description 4
- 210000003710 cerebral cortex Anatomy 0.000 claims description 6
- 230000002490 cerebral effect Effects 0.000 claims description 5
- 239000002639 bone cement Substances 0.000 claims description 3
- 210000001951 dura mater Anatomy 0.000 claims description 3
- 239000000284 extract Substances 0.000 claims description 3
- 238000009958 sewing Methods 0.000 claims description 3
- 210000004872 soft tissue Anatomy 0.000 claims description 3
- 238000005516 engineering process Methods 0.000 abstract description 3
- 210000003128 head Anatomy 0.000 abstract description 3
- 238000010171 animal model Methods 0.000 abstract description 2
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 238000005553 drilling Methods 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 230000004083 survival effect Effects 0.000 abstract 1
- 238000010992 reflux Methods 0.000 description 3
- 230000007812 deficiency Effects 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 108010025020 Nerve Growth Factor Proteins 0.000 description 1
- 102000007072 Nerve Growth Factors Human genes 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 208000015114 central nervous system disease Diseases 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 239000003900 neurotrophic factor Substances 0.000 description 1
Landscapes
- Infusion, Injection, And Reservoir Apparatuses (AREA)
Abstract
The invention discloses a method for long-term intracerebral pump injection of drugs in the great quantity used for a primate. The method comprises following steps: step1, positioning and opening a skull of an animal-determining an injection position and drilling the animal skull after general anesthesia is carried out to the animal; step2, imbedding a silica gel syringe needle into a brain; step3, pulling out a hard metal needle core of a silica gel syringe needle; step 4, connecting with a micro-pressure pump and starting intracerebral injection of a drug solution or an experiment reagent; step5, pulling out the silica gel syringe needle after finishing injection. In the step 5, the silica gel syringe needle is not pulled out in need of multiple dosing on a long-term basis. The micro-pressure pump is embedded into the skull surface under the head skin and an injection syringe is utilized for puncturing the scalp and injecting drugs into the micro-pressure pump for multiple drug injection on a long-term basis. The method for long-term intracerebral pump injection of drugs in the great quantity used for the primate has following beneficial effects: technology of pump injection of drugs is safe and reliable so as to effectively avoid intracerebral mass effect and effectively avoid countercurrent flow of drugs in case of multiple drug injection; intracerebral dosage is carried out for multiple times; and skull-opening operation is avoided for injection at each time so as to greatly simplify procedures for experimental operation, thereby improving survival quality of an experimental animal and scientific credibility of experimental data.
Description
Technical field
The present invention relates to a kind of pump medicine technology, be specifically related to a kind of method for volume pump medicine in primate long term brain.
Background technology
In the research of primate central nervous system disease, when needs are to drugs such as intracerebral injection cell or neurotrophic factors, microsyringe is normally utilized to carry out under MRI orientation direction, extra pressure can not be applied during injection, microsyringe and injection needle are rigidity, injection needle is without tube core, and different according to injection target position, per injection amount can reach 5 ~ 20 microlitres at the most.
There are following 2 deficiencies: 1, per injection amount only about 20 microlitres at the most when utilizing microsyringe to carry out intracerebral injection, easily form occupation time process when injection volume is more at target position place or make because pressure is high medicine go out outside brain along needle track reflux, forming secondary damage or cause dosage not enough; 2, use microsyringe can not continue medication for a long time when carrying out intracerebral injection, per injection all needs out cranium to carry out, and operates more loaded down with trivial details and risk is larger.
Summary of the invention
The object of the invention is to for the deficiencies in the prior art, provide a kind of safe and reliable, the method for volume pump medicine in primate long term brain that effectively can avoid the generation of occupation time process in brain, administration in brain can be carried out repeatedly.
To achieve these goals, present invention employs following technical scheme:
For a method for volume pump medicine in primate long term brain, comprise the following steps:
Step one, after animal general anesthesia, utilizes stereotaxic technique determination injection site and holes on animal skull;
Step 2, uses stereotaxic instrument that the silica gel injection needle containing hard metal nook closing member is entered Cerebral cortex through sphenotresia place puncture cerebral dura mater, to enter after Cerebral cortex with the speed inserting needle of 1 mm/min until needle point arrives target position, leaves standstill stop 10 minutes;
Step 3, extracts the hard metal nook closing member of silica gel injection needle, then allows silica gel injection needle leave standstill stop 10 minutes;
Step 4, connects minute-pressure pump (can single or continue medication), starts to intracerebral injection drug solution or experiment reagent by one end that silica gel injection needle exposes outside brain, during single injection, injection speed is 1 ~ 10 mul/min (adjusting according to target position difference);
Step 5, during single injection, injected rear silica gel injection needle and minute-pressure pump leaves standstill stop 10 minutes, and then utilized stereotaxic instrument slowly to be extracted by silica gel injection needle, speed of extracting is identical with inserting needle speed, is all 1 mm/min;
When the long-term multiple dosing of needs, silica gel injection needle is not extracted after completing first time injection, with the boring on bone cement shutoff skull and fixing silica gel injection needle, and then minute-pressure pump screw special is fixed on skull surface also with skin and sub-dermal soft tissue covering, sewing up and close operation wound chamber, is that after usable syringes puncture scalp, in minute-pressure pump, filling medicine carries out long-term repeatedly drug injection afterwards.
Volume pump prescription method of the present invention have employed convection current and strengthens injection technique (CED), namely utilizes minute-pressure pump and the silica gel injection needle containing hard metal nook closing member to replace microsyringe to inject; Silica gel injection needle is non-rigid, has certain elasticity and pliability, injects and enters in cerebral tissue and can fit tightly with cerebral tissue after pulling out tube core, is beneficial to and stops medicine along needle track reflux; Certain pressure can be put on the liquid (medicine or cell suspension) in silica gel syringe needle by minute-pressure pump, it is helped slowly to spread at target position place along needle point and be unlikely to be formed occupation time process, thus the medicine of injectable more amount, minute-pressure pump can be imbedded at skull surface under animal head muscle where necessary, facilitates continuous several times administration.
Advantage of the present invention:
1. convection current strengthens injection technique relative usage microsyringe and carries out the generation that injection effectively can avoid occupation time process in brain.
2. convection current strengthens medicine reflux when injection technique can effectively avoid volume to inject.
3. minute-pressure pump to be imbedded under skin of head after skull surface, only need can carry out repeatedly administration in brain with the syringe scalp medicine of annotating in pump that punctures, and all open cranium without the need to per injection and carry out, very big simplification experimental implementation flow process, improves laboratory animal life quality and experimental data science credibility.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further described.
Embodiment:
For a method for volume pump medicine in primate long term brain, comprise the following steps:
Step one, after animal general anesthesia, utilizes stereotaxic technique determination injection site and holes on animal skull;
Step 2, uses stereotaxic instrument that the silica gel injection needle containing hard metal nook closing member is entered Cerebral cortex through sphenotresia place puncture cerebral dura mater, to enter after Cerebral cortex with the speed inserting needle of 1 mm/min until needle point arrives target position, leaves standstill stop 10 minutes;
Step 3, extracts the hard metal nook closing member of silica gel injection needle, then allows silica gel injection needle leave standstill stop 10 minutes;
Step 4, connects minute-pressure pump by one end that silica gel injection needle exposes outside brain, starts to intracerebral injection drug solution or experiment reagent, and during single injection, injection speed is 1 ~ 10 mul/min (adjusting according to target position difference);
Step 5, during single injection, injected rear silica gel injection needle and minute-pressure pump leaves standstill stop 10 minutes, and then utilized stereotaxic instrument slowly to be extracted by silica gel injection needle, speed of extracting is identical with inserting needle speed, is all 1 mm/min;
When the long-term multiple dosing of needs, silica gel injection needle is not extracted after completing first time injection, with the boring on bone cement shutoff skull and fixing silica gel injection needle, and then minute-pressure pump screw special is fixed on skull surface also with skin and sub-dermal soft tissue covering, sewing up and close operation wound chamber, is that after usable syringes puncture scalp, in minute-pressure pump, filling medicine carries out long-term repeatedly drug injection afterwards.
Claims (1)
1., for a method for volume pump medicine in primate long term brain, it is characterized in that, comprise the following steps:
Step one, after animal general anesthesia, utilizes stereotaxic technique determination injection site and holes on animal skull;
Step 2, uses stereotaxic instrument that the silica gel injection needle containing hard metal nook closing member is entered Cerebral cortex through sphenotresia place puncture cerebral dura mater, to enter after Cerebral cortex with the speed inserting needle of 1 mm/min until needle point arrives target position, leaves standstill stop 10 minutes;
Step 3, extracts the hard metal nook closing member of silica gel injection needle, then allows silica gel injection needle leave standstill stop 10 minutes;
Step 4, connects minute-pressure pump by one end that silica gel injection needle exposes outside brain, starts to intracerebral injection drug solution or experiment reagent, and during single injection, injection speed is 1 ~ 10 mul/min;
Step 5, during single injection, injected rear silica gel injection needle and minute-pressure pump leaves standstill stop 10 minutes, and then utilized stereotaxic instrument slowly to be extracted by silica gel injection needle, speed of extracting is identical with inserting needle speed, is all 1 mm/min; When the long-term multiple dosing of needs, silica gel injection needle is not extracted after completing first time injection, with the boring on bone cement shutoff skull and fixing silica gel injection needle, and then minute-pressure pump screw special is fixed on skull surface also with skin and sub-dermal soft tissue covering, sewing up and close operation wound chamber, is that after usable syringes puncture scalp, in minute-pressure pump, filling medicine carries out long-term repeatedly drug injection afterwards.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510414409.2A CN104971400A (en) | 2015-07-15 | 2015-07-15 | Method for long-term intracerebral pump injection of drugs in great quantity used for primate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510414409.2A CN104971400A (en) | 2015-07-15 | 2015-07-15 | Method for long-term intracerebral pump injection of drugs in great quantity used for primate |
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| Publication Number | Publication Date |
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| CN104971400A true CN104971400A (en) | 2015-10-14 |
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|---|---|---|---|
| CN201510414409.2A Pending CN104971400A (en) | 2015-07-15 | 2015-07-15 | Method for long-term intracerebral pump injection of drugs in great quantity used for primate |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113907909A (en) * | 2021-10-27 | 2022-01-11 | 苏州大学 | Brain localization injection device and injection method for animal experiment |
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|---|---|---|---|---|
| CN1597000A (en) * | 2004-07-21 | 2005-03-23 | 张商钱 | Anti-countercurrent arteriovenous retention needle |
| CN201061556Y (en) * | 2007-07-26 | 2008-05-21 | 赵高旭 | Minimally invasive ventricular puncture trocar |
| US20090143764A1 (en) * | 2007-11-30 | 2009-06-04 | Medtronic, Inc. | Infusion catheter assembly with reduced backflow |
| CN201299855Y (en) * | 2008-10-22 | 2009-09-02 | 中国人民解放军第二军医大学 | Drug feed device used for animal in vivo experiments of drug induced infusion phlebitis |
| CN201437014U (en) * | 2009-05-07 | 2010-04-14 | 上海交通大学医学院附属新华医院 | A kind of puncture and drainage device for children's halo door |
| CN201612673U (en) * | 2009-07-20 | 2010-10-27 | 余斌 | Improved peripheral nerve block retaining trocar |
| CN202342649U (en) * | 2011-10-26 | 2012-07-25 | 李德凤 | Micro-pump-dosed brachial plexus anesthesia puncture needle |
| CN203139250U (en) * | 2013-04-04 | 2013-08-21 | 吴慧 | Novel trace analgesia pump |
| CN103861172A (en) * | 2012-12-18 | 2014-06-18 | 珠海凯迪莱医疗科技有限公司 | Disposable pneumatic dual-tube insulin injection pump |
| CN104225772A (en) * | 2014-09-25 | 2014-12-24 | 山西医科大学 | Visual minimally-invasive intracranial dosing device |
| CN104582610A (en) * | 2012-07-24 | 2015-04-29 | 瑞尼斯豪公司 | Neurosurgical apparatus and methods |
-
2015
- 2015-07-15 CN CN201510414409.2A patent/CN104971400A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1597000A (en) * | 2004-07-21 | 2005-03-23 | 张商钱 | Anti-countercurrent arteriovenous retention needle |
| CN201061556Y (en) * | 2007-07-26 | 2008-05-21 | 赵高旭 | Minimally invasive ventricular puncture trocar |
| US20090143764A1 (en) * | 2007-11-30 | 2009-06-04 | Medtronic, Inc. | Infusion catheter assembly with reduced backflow |
| CN201299855Y (en) * | 2008-10-22 | 2009-09-02 | 中国人民解放军第二军医大学 | Drug feed device used for animal in vivo experiments of drug induced infusion phlebitis |
| CN201437014U (en) * | 2009-05-07 | 2010-04-14 | 上海交通大学医学院附属新华医院 | A kind of puncture and drainage device for children's halo door |
| CN201612673U (en) * | 2009-07-20 | 2010-10-27 | 余斌 | Improved peripheral nerve block retaining trocar |
| CN202342649U (en) * | 2011-10-26 | 2012-07-25 | 李德凤 | Micro-pump-dosed brachial plexus anesthesia puncture needle |
| CN104582610A (en) * | 2012-07-24 | 2015-04-29 | 瑞尼斯豪公司 | Neurosurgical apparatus and methods |
| CN103861172A (en) * | 2012-12-18 | 2014-06-18 | 珠海凯迪莱医疗科技有限公司 | Disposable pneumatic dual-tube insulin injection pump |
| CN203139250U (en) * | 2013-04-04 | 2013-08-21 | 吴慧 | Novel trace analgesia pump |
| CN104225772A (en) * | 2014-09-25 | 2014-12-24 | 山西医科大学 | Visual minimally-invasive intracranial dosing device |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113907909A (en) * | 2021-10-27 | 2022-01-11 | 苏州大学 | Brain localization injection device and injection method for animal experiment |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20151014 |
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