CN104872155A - Compound bactericide capable of preventing and treating rice blast - Google Patents
Compound bactericide capable of preventing and treating rice blast Download PDFInfo
- Publication number
- CN104872155A CN104872155A CN201510332882.6A CN201510332882A CN104872155A CN 104872155 A CN104872155 A CN 104872155A CN 201510332882 A CN201510332882 A CN 201510332882A CN 104872155 A CN104872155 A CN 104872155A
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- CN
- China
- Prior art keywords
- rice blast
- albendazole
- tricyclazole
- bactericide
- compound
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 36
- 235000009566 rice Nutrition 0.000 title claims abstract description 36
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 21
- 239000003899 bactericide agent Substances 0.000 title claims abstract description 20
- 150000001875 compounds Chemical class 0.000 title claims abstract description 20
- 240000007594 Oryza sativa Species 0.000 title claims 2
- HXHWSAZORRCQMX-UHFFFAOYSA-N albendazole Chemical compound CCCSC1=CC=C2NC(NC(=O)OC)=NC2=C1 HXHWSAZORRCQMX-UHFFFAOYSA-N 0.000 claims abstract description 27
- 229960002669 albendazole Drugs 0.000 claims abstract description 27
- DQJCHOQLCLEDLL-UHFFFAOYSA-N tricyclazole Chemical compound CC1=CC=CC2=C1N1C=NN=C1S2 DQJCHOQLCLEDLL-UHFFFAOYSA-N 0.000 claims abstract description 26
- 239000000463 material Substances 0.000 claims abstract description 8
- 239000000645 desinfectant Substances 0.000 claims description 14
- 239000003814 drug Substances 0.000 claims description 8
- 229940079593 drug Drugs 0.000 claims description 5
- 241000209094 Oryza Species 0.000 abstract description 34
- 230000000694 effects Effects 0.000 abstract description 10
- 230000002195 synergetic effect Effects 0.000 abstract description 5
- 241000894006 Bacteria Species 0.000 abstract description 2
- 231100000614 poison Toxicity 0.000 abstract 2
- 230000007096 poisonous effect Effects 0.000 abstract 2
- 239000004557 technical material Substances 0.000 abstract 2
- 241001330975 Magnaporthe oryzae Species 0.000 description 15
- 238000012360 testing method Methods 0.000 description 8
- 206010039509 Scab Diseases 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 231100000820 toxicity test Toxicity 0.000 description 5
- 241001344131 Magnaporthe grisea Species 0.000 description 4
- 241000607479 Yersinia pestis Species 0.000 description 4
- 239000002131 composite material Substances 0.000 description 4
- 230000003902 lesion Effects 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 239000007921 spray Substances 0.000 description 3
- 230000001988 toxicity Effects 0.000 description 3
- 231100000419 toxicity Toxicity 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 241000231139 Pyricularia Species 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 2
- 229920000053 polysorbate 80 Polymers 0.000 description 2
- 230000003449 preventive effect Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000193764 Brevibacillus brevis Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000220324 Pyrus Species 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 230000000843 anti-fungal effect Effects 0.000 description 1
- 229940121375 antifungal agent Drugs 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000000205 computational method Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 210000005069 ears Anatomy 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000000855 fungicidal effect Effects 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 150000002460 imidazoles Chemical class 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 239000012452 mother liquor Substances 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 235000021017 pears Nutrition 0.000 description 1
- 239000003415 peat Substances 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000004763 spore germination Effects 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 229910052902 vermiculite Inorganic materials 0.000 description 1
- 235000019354 vermiculite Nutrition 0.000 description 1
- 239000010455 vermiculite Substances 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a compound bactericide capable of preventing and treating rice blast. The compound bactericide is prepared from base materials albendazole and tricyclazole in the mass ratio of 1: (15-35); the albendazole is a bactericide technical material in the mass fraction of 97%, while the tricyclazole is a bactericide technical material in the mass fraction of 95%. Preferably, the mass ratio of the albendazole and tricyclazole is 1: 35. According to the compound bactericide, the two base materials are not chemically reacted, have an obvious synergic or addition effect, and are safe to use; besides, the compound bactericide has excellent poisonous activity on the rice blast bacteria different in types in the field at present, and is obviously higher than the single dose of the albendazole or the tricyclazole in the poisonous activity. The compound bactericide is suitable for vast rural areas to prevent and treat the rice blast.
Description
Technical field
The present invention is relevant with bactericide, specifically, belongs to the bactericide containing organic compound, furthermore, belongs to the bactericide of control rice blast.
Technical background
Rice blast fungus (Pyricularia oryae Cav.), another name rice blast, burns pest, pest of kowtowing, hangs neck pest, and be one of Major Diseases of paddy rice, can cause the significantly underproduction, the underproduction 40% ~ 50% time serious, even No kernels or seeds are gathered, as in a year of scarcity.Rice blast all has generation all over the world, wherein occurs as many with leaf portion, joint portion, can cause the underproduction in various degree after generation, and especially panicle blast or joint pest occur early and weigh, and can cause dead ears so that total crop failure.Rice blast is caused by Deuteromycotina fungi Invisible element ash pears spore Pyricularia grisea (Cooke) Sacc, Perfect stage Magnaporthe grisea Pyricularia oryzae Cav.
Albendazole and Albendazole (Albendazole), a kind of efficient, safety, broad spectrum activity imidazoles bactericide, significant preventive effect is had to plurality of plant diseases, and have certain production-increasing function, certain effect is had to control rice blast fungus, but along with the increase of paddy rice Antifungal resistance, effect weakens to some extent; Tricyclazole (tricyclazole) is control rice blast bactericide specially, and the mechanism of action mainly suppresses the melanic formation of attachment spore, thus suppresses spore germination and attachment spore to be formed, and stops germ to invade people and reduces the generation of magnaporthe grisea spore.
In recent years, the problem of control rice blast is subject to people's attention, develop many relevant bactericide, such as: No. 2004100143497, Chinese patent " a kind of built agent preventing and treating rice blast ", No. 2007101868948 " a kind of bactericidal composition preventing and treating rice blast ", No. 2007100215094 " active bacteria formulation of Brevibacillus brevis TW and control rice blast ", No. 2011100704006 " bacteriocide formula of control rice blast and usings method thereof " etc.How these fungicide effects are still not clear.There is no at present with albendazole and tricyclazole composite, to the related application part of the bactericide of rice Pyricularia grisea.
Summary of the invention
The object of the present invention is to provide a kind of compound disinfectant preventing and treating rice blast, it possesses rice blast fungus stronger kills ability, can be applied to control rice blast, the increasing production of rice accounting for agricultural product critical role is increased income, be the development service of national economy.
Inventor carries out repeated screening to having the agricultural chemicals killing rice blast fungus activity, and repetition test is carried out to the bulk drug quality proportioning of Mixed Pharmacy, result of the test is contrasted, finds a kind of compound disinfectant, stronger poisoning ability is possessed to rice blast fungus.
The compound disinfectant of the control rice blast that inventor provides is with albendazole and tricyclazole for base-material, the quality proportioning 1 according to albendazole and tricyclazole: the compound disinfectant that (15 ~ 35) are formulated; Described albendazole is the bactericide original drug of mass fraction 97%, and above-mentioned tricyclazole is the bactericide original drug of mass fraction 95%.
Through preferred further, the best in quality proportioning of albendazole and tricyclazole is 1: 35.
The screening test of the compound disinfectant of above-mentioned control rice blast is as follows:
1 test objective: adopt indoor living pot-culture method, test albendazole, tricyclazole single dose is active to the Indoor antibacterial of rice blast fungus, the synergy type of design and screening 5 proportioning combinations, send out the proportioning now with addition or synergistic effect, for field trial and next step research and development provide foundation.
2 test materials: the former medicine of 97% albendazole is provided by Guizhou Daoyuan Biotechnology Co., Ltd.; 95% Mensurating Tricyclazole Technical is provided by pesticide research center, south, Jiangsu.
3 test dose designs:
Devise totally 5 combinations such as 1: 15,1: 20,1: 25,1: 30 and 1: 35.
4 for examination target
Rice blast fungus (Pyricularia oryae Cav.), indoor Plantlet in vitro and cultivation.
Rice varieties: Suyunuo, rice blast height sense kind, plant in basin, matrix is peat soil: vermiculite (3: 1), grows to 3 true leaves for subsequent use.
5 test methods
Medicament is prepared: albendazole DMF dissolves, and tricyclazole acetone solution, is mixed with mother liquor.According to experimental scheme with 0.1% Tween-80 stepwise dilution.
Chemicals treatment: set and only spray clear water as blank.Spraying adopts ACO series electromagnetic type air compressor to drive 10ml glass sprayer, and liquid is evenly imposed on rice seedlings complete stool, every basin spray 3ml liquid.Often process and repeat for 3 times.
Inoculation and cultivation: Pyricularia oryzae is forwarded in advance to produce on spore medium and produces spore, with L-type glass bar scraping spore, with two-layer filtered through gauze spore suspension; 0.05% Tween 80 emulsified water regulates concentration to 1 × 10
8individual spore/ml.
After spray medicine 24h, the Fresh spores uniform suspension prepared is inoculated in vacuum side of blade, inoculation adopts ACO series electromagnetic type air compressor to drive 10ml glass sprayer, every basin 2ml spore suspension.Postvaccinal rice seedlings moves in moisture preserving cover in time, and with humidifier timing humidification, prevent spore suspension from killing, shading, temperature controls at 25 DEG C.After 24h, rice seedlings moves into climatic cabinate, keeps temperature 28 DEG C, and RH 85%, 30000LUX illumination 16h, dark 8h are alternately.
Investigation method: when the sick leaf rate of blank reaches more than 50%, classification investigation the 2nd, 3 leaf incidences, grade scale is (in units of leaf, with reference to relevant rice blast grade scale and classification collection of illustrative plates).
0 grade: without scab; 1 grade: occur brown some scab;
3 grades: occur typical spindle scab, lesion area accounts for whole leaf area less than 5%;
5 grades: typical scab, lesion area accounts for 6 ~ 25% of whole leaf area;
7 grades: typical scab, lesion area accounts for 26 ~ 50% of whole leaf area;
9 grades: lesion area accounts for more than 51% of whole leaf area.
Computational methods: according to survey data, the disease calculating each process refers to and control efficiency.
Evaluation method: co-toxicity coefficient method
The content of toxicity index × B in mixture (%) of the content of toxicity index × A in mixture (the %)+B of theoretical toxicity index TTI=A
Co-toxicity coefficient is greater than 120, shows there is synergistic effect; Be less than 80 and show there is antagonism; Show there is summation action between 80-120.
6 toxicity tests
The toxicity test of single dose
Albendazole, tricyclazole are to the toxicity test of rice blast
Albendazole is to the EC of rice blast fungus
50for 1741.877mg/L, in this test, do not make the preventive effect of 90%, its EC
90value is greater than 2000mg/L, and tricyclazole is to the EC of rice blast fungus
50, EC
90value is respectively 1.2284mg/L, 17.6383mg/L.
5 proportionings are to the toxicity test of rice blast
5 composite proportionings are to the toxicity test result of rice blast fungus
The EC of proportioning 1: 25,1: 30 and 1: 35
50value is 0.9193 ~ 1.1573mg/L, the active EC higher than two single dose proportionings 1: 15 and 1: 20
50value, is respectively 1.6293mg/L and 1.5197mg/L, active between two single doses; The EC of proportioning 1: 15,1: 20 and 1: 30
90value is 17.6437 ~ 27.1839mg/L, active between two single doses, the EC of proportioning 1: 25 and 1: 35
90value is respectively 13.6504mg/L, 12.8628mg/L, active in two single doses.
Synergy is evaluated
5 composite proportionings of albendazole and tricyclazole are to the synergy evaluation of rice blast
The co-toxicity coefficient of proportioning 1: 35 is 137.44, is greater than 120 and shows as synergistic effect; The co-toxicity coefficient of other 4 proportionings is 80.42 ~ 110.39, shows as summation action.
7 conclusions
Albendazole is effective to rice blast fungus, and tricyclazole has good bacteriostatic activity to rice blast fungus.After both are composite, there is addition or synergistic effect.In conjunction with EC
50, EC
90, 3 indexs such as co-toxicity coefficient, 5 proportionings all can be selected, with proportioning albendazole: tricyclazole is 1:35 is good.
Compound disinfectant of the present invention, there is not chemical reaction in two kinds of base-materials, has obvious synergy or be added effect, use safety, good cytotoxicity is had for the rice blast fungus that current field is dissimilar, and apparently higher than albendazole or tricyclazole single dose.Be applicable to vast rural area for preventing and treating rice blast.
Embodiment
Execute in fact routine ︰ and get the albendazole 2kg of mass fraction 97% and the tricyclazole 70kg of mass fraction 95% is base-material, be re-dubbed the compound disinfectant that 72kg prevents and treats rice blast.
This compound disinfectant uses in somewhere, Guizhou for 2015, obtains the effect of control rice blast very well.
Claims (2)
1. prevent and treat a compound disinfectant for rice blast, it is characterized in that this compound disinfectant is with albendazole and tricyclazole for base-material, the quality proportioning 1 according to albendazole and tricyclazole: the compound disinfectant that (15 ~ 35) are formulated;
Described albendazole is the bactericide original drug of mass fraction 97%, and above-mentioned tricyclazole is the bactericide original drug of mass fraction 95%.
2., according to compound disinfectant according to claim 1, it is characterized in that this compound disinfectant is with albendazole and tricyclazole for base-material, formulated according to the quality proportioning of albendazole and tricyclazole 1: 35.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510332882.6A CN104872155B (en) | 2015-06-16 | 2015-06-16 | A kind of compound disinfectant preventing and treating rice blast |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510332882.6A CN104872155B (en) | 2015-06-16 | 2015-06-16 | A kind of compound disinfectant preventing and treating rice blast |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104872155A true CN104872155A (en) | 2015-09-02 |
| CN104872155B CN104872155B (en) | 2016-09-21 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510332882.6A Active CN104872155B (en) | 2015-06-16 | 2015-06-16 | A kind of compound disinfectant preventing and treating rice blast |
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| Country | Link |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106719747A (en) * | 2017-03-02 | 2017-05-31 | 贵州道元生物技术有限公司 | By rosickyite azoles and the bactericide of tricyclazole compounding preventing and treating rice blast |
| CN107333791A (en) * | 2017-08-30 | 2017-11-10 | 贵州道元生物技术有限公司 | By rosickyite azoles and the bactericide of the preventing and treating rice blast of OIT compounding |
| CN107333792A (en) * | 2017-08-30 | 2017-11-10 | 贵州道元生物技术有限公司 | By rosickyite azoles and the bactericide of the preventing and treating rice blast of miaow chaff azoles compounding |
| CN113632795A (en) * | 2021-05-07 | 2021-11-12 | 广东省农业科学院果树研究所 | Application of albendazole sulfoxide in preparation of pesticide bactericide for preventing and treating citrus postharvest chloromycete |
| CN113662002A (en) * | 2021-05-07 | 2021-11-19 | 广东省农业科学院果树研究所 | Application of albendazole in preparation of pesticide bactericide for preventing and treating rice blast disease |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1518876A (en) * | 2003-01-23 | 2004-08-11 | 王玉万 | Bactericide and insecticide in agricultural use containing albendazole sulfoxide and its salt |
| CN104106580A (en) * | 2014-06-03 | 2014-10-22 | 贵州道元生物技术有限公司 | Compound bactericide for preventing and treating wheat scab |
-
2015
- 2015-06-16 CN CN201510332882.6A patent/CN104872155B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1518876A (en) * | 2003-01-23 | 2004-08-11 | 王玉万 | Bactericide and insecticide in agricultural use containing albendazole sulfoxide and its salt |
| CN104106580A (en) * | 2014-06-03 | 2014-10-22 | 贵州道元生物技术有限公司 | Compound bactericide for preventing and treating wheat scab |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106719747A (en) * | 2017-03-02 | 2017-05-31 | 贵州道元生物技术有限公司 | By rosickyite azoles and the bactericide of tricyclazole compounding preventing and treating rice blast |
| CN107333791A (en) * | 2017-08-30 | 2017-11-10 | 贵州道元生物技术有限公司 | By rosickyite azoles and the bactericide of the preventing and treating rice blast of OIT compounding |
| CN107333792A (en) * | 2017-08-30 | 2017-11-10 | 贵州道元生物技术有限公司 | By rosickyite azoles and the bactericide of the preventing and treating rice blast of miaow chaff azoles compounding |
| CN113632795A (en) * | 2021-05-07 | 2021-11-12 | 广东省农业科学院果树研究所 | Application of albendazole sulfoxide in preparation of pesticide bactericide for preventing and treating citrus postharvest chloromycete |
| CN113662002A (en) * | 2021-05-07 | 2021-11-19 | 广东省农业科学院果树研究所 | Application of albendazole in preparation of pesticide bactericide for preventing and treating rice blast disease |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104872155B (en) | 2016-09-21 |
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