CN110495456A - A kind of bactericidal composition - Google Patents
A kind of bactericidal composition Download PDFInfo
- Publication number
- CN110495456A CN110495456A CN201810472191.XA CN201810472191A CN110495456A CN 110495456 A CN110495456 A CN 110495456A CN 201810472191 A CN201810472191 A CN 201810472191A CN 110495456 A CN110495456 A CN 110495456A
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- Prior art keywords
- polyglutamic acid
- disease
- agent
- acid
- supplied
- Prior art date
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
- A01N31/16—Oxygen or sulfur directly attached to an aromatic ring system with two or more oxygen or sulfur atoms directly attached to the same aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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Abstract
The invention belongs to a kind of technical field of pesticide, disclose a kind of bactericidal composition, one kind is principle active component using polyglutamic acid and in for amino-oligosaccharide, eugenol, humic acid copper, Brevibacillus laterosporus, bacillus subtilis, waxy Bacillus, bacillus licheniformis, the two mass ratio is 1:20~20:1, and quality hundred content of the sum of the two in bactericidal composition is 0.1-50%.The invention also discloses the bactericidal compositions can be prepared into aqueous suspension agent, wettable powder, water dispersible granules, granule, and for preventing and kill off powdery mildew, angular leaf spot, head blight, late blight, wilt disease, false smut, rice blast, bacterial wilt, soft rot, banded sclerotial blight, verticillium wilt, balck shank, anthracnose, late blight, gray mold, downy mildew, bacterial leaf-blight, epidemic disease, virosis, scab, rot disease, foot rot, full rot.Bactericidal composition of the invention is applied widely, prevent and prevention and treatment bactericidal effect is good.
Description
Technical field
The invention belongs to technical field of pesticide, especially fungicide field, and in particular to a kind of sterilization containing polyglutamic acid
Composition.
Background technique
Polyglutamic acid is the product of microorganism (predominantly bacillus category) fermentation, is a kind of extracellular polypeptide, is to be at present
Only it is only can be polymerize as microorganism obtained from 4 kinds of polymeric amino acids (poly-aspartate, gamma-polyglutamic acid, poly ornithine,
One of epsilon-polylysine).A kind of water-soluble high-molecular material of the polyglutamic acid as novel biodegradable has moisturizing, increases
The characteristics such as thick, film forming, bonding, nontoxic, water-soluble, biodegradable, are widely used it in pesticide field.
Amino-oligosaccharide is to degrade again obtained after obtaining chitosan by chitin degrading, or is extracted by microbial fermentation, right
The growth of some germs generates inhibiting effect, influences fungus spore germination, induction hypha form morphs, biochemistry occurs in spore
Change etc., while plant can be excited to generate the chitinase with resistant effect, dextranase, guarantor's element and PR albumen etc., and have
Cell activation effect facilitates the recovery of aggrieved plant, and promoting root growth strong sprout enhances the resistance of crop, promotes plant growth and development.
Not only there is extremely strong preventive effect, but also have effects that nutrition, adjusting, removing toxic substances to fungi, bacterium, virus.It can be widely used for
Virus, bacterium, the fungus-caused disease for preventing and treating fruit tree, vegetables, underground rhizome, tobacco, Chinese medicine and grain and cotton crop.
Eugenol is botanical pesticide, which is to extract sterilization component from the plants such as cloves, is aided with a variety of auxiliary agents and grinds
Novel low-toxicity fungicide made of system can effectively prevent gray mold, downy mildew to people and animals and Environmental security.
Humic acid copper is the chelate of humic acid, is that a kind of polynary organic acid mixes, they can with metal from
Son reaction forms chelate, these chelates chemical substance in alkaline solution is more stable, not only there is stronger sterilizing ability,
And without phytotoxicity.
Brevibacillus laterosporus is a kind of new type bactericide, can prevent and treat multiple diseases, such as capsicum epidemic disease, watermelon are withered
Disease, wheat scab, grape grey mould, Strawberry anthracnose.
Bacillus subtilis is a kind of microbial bactericide, passes through the raw antibacterial material of effective component producing bacillus subtilis
With the mode of action of site competition, induction of resistance, kills and control pathogen, to achieve the purpose that controlling disease.
Waxy Bacillus improves crop and endangers generation oxygen in primosome to germ and adverse circumstance by intracorporal SOD enzyme
Scavenging activity adjusts crop cell microhabitat, the normal physiological metabolism of maintenance cell and biochemical reaction, improves resistance, accelerate
Growth, improves the yield and quality.
Bacillus licheniformis is microbial bactericide, is that bacillus licheniformis is prevented using bacillary made of culture medium fermentation
Sick preparation has strong antagonism to plant pathogenic fungi class, can effectively prevent cucumber and tobacco diseases.But as life
Though the bacillus licheniformis lasting period of object pesticide is longer, the quick-acting of product is poor.
So far by polyglutamic acid and new type bactericide amino-oligosaccharide, eugenol, humic acid copper, the short gemma of side spore
Bacillus, bacillus subtilis, waxy Bacillus, bacillus licheniformis compounding, both at home and abroad there is not yet relevant report, and inventor
Research finds that the two compounding has good synergistic effect in a certain range, while preventing and treating disease, reaches volume increase
Effect.
Based on the above situation, the purpose of the present invention is to provide a kind of bactericidal composition and its preparations.The bactericidal composition
Prevention and treatment with bactericidal effect is good, stability is good, persistence is good, can effectively prevent powdery mildew, angular leaf spot, head blight, late blight,
Wilt disease, false smut, rice blast, bacterial wilt, soft rot, banded sclerotial blight, verticillium wilt, balck shank, anthracnose, late blight, gray mold,
Downy mildew, bacterial leaf-blight, epidemic disease, virosis, scab, rot disease, foot rot, full rot, aqueous suspension agent can be prepared into, can
Wet powder, water dispersible granules, granule.
To achieve the purpose of the present invention, the invention provides the following technical scheme:
A kind of bactericidal composition, it is characterised in that the bactericidal composition includes effective constituents A and B, and A is polyglutamic acid, and B is
Amino-oligosaccharide, eugenol, humic acid copper, Brevibacillus laterosporus, bacillus subtilis, waxy Bacillus, lichens bud
The mass ratio of one of spore bacillus, A and B are 1:40~40:1, preferably 1:20~20:1, and sum of the two accounts for bactericidal composition
Percentage composition be 0.1-50%.
In two-spot compound preparation of the invention, other than effective component, the auxiliary agent allowed on pesticide can be also added, to prepare
At any one dosage form.It is preferred that being processed into wettable powder, water dispersible granules, granule, aqueous suspension agent.The insecticides adjuvant
Including dispersing agent, wetability, thickener, antifreezing agent, preservative, thickener etc., using well known to a person skilled in the art auxiliary agents.
Instant component is reasonable, and effect is good, and its activity and the active simple superposition of bactericidal effect not instead of each component,
With significant synergistic effect, to crop safety, there is excellent control efficiency to corps diseases.
Specific embodiment
Polyglutamic acid amino-oligosaccharide
12% polyglutamic acid amino-oligosaccharide wettable powder of S1
Polyglutamic acid 5%, amino-oligosaccharide 7%, dispersing agent sodium lignin sulfonate 1.5%, wetting agent detergent alkylate sulphur
Sour sodium 1%, filler clay are supplied.It is made according to conventional preparation method.
10% polyglutamic acid amino-oligosaccharide wettable powder of S2
Polyglutamic acid 9.5%, amino-oligosaccharide 0.5%, dispersing agent sodium polycarboxylate 1.5%, wetting agent dodecyl sulphate
Sodium 1%, filler bentonite are supplied.It is made according to conventional preparation method.
6% polyglutamic acid amino-oligosaccharide wettable powder of S3
Polyglutamic acid 0.5%, amino-oligosaccharide 5.5%, dispersing agent Negel 0.3%, wetting agent dodecyl
Benzene sulfonic acid sodium salt 0.2%, filler attapulgite are supplied.It is made according to conventional preparation method.
30% polyglutamic acid amino-oligosaccharide water dispersible granules of S4
Polyglutamic acid 2%, amino-oligosaccharide 28%, dispersing agent sodium naphthalene sulfonate 3%, wetting agent lauryl sodium sulfate
2.5%, disintegrating agent ammonium sulfate 4.5%, binder starch 2%, filler diatomite are supplied.It is made according to conventional preparation method.
22% polyglutamic acid amino-oligosaccharide water dispersible granules of S5
The wooden disulfonate acid 1.5% of polyglutamic acid 4%, amino-oligosaccharide 18%, dispersing agent, wetting agent dodecyl sulphate
Sodium 0.5%, disintegrating agent sodium sulphate 3%, binder methylcellulose 1%, filler clay are supplied.According to conventional preparation method system
.
18% polyglutamic acid amino-oligosaccharide water dispersible granules of S6
The wooden disulfonate acid 1% of polyglutamic acid 17%, amino-oligosaccharide 1%, dispersing agent, wetting agent lauryl sodium sulfate
0.5%, disintegrating agent ammonium sulfate 2.5%, binder glucose 0.5%, filler attapulgite are supplied.According to conventional preparation method system
.
8% polyglutamic acid amino-oligosaccharide granule of S7
Polyglutamic acid 0.5%, amino-oligosaccharide 7.5%, adhesive dextrin 7%, filler attapulgite are supplied.According to routine
Formulation method is made.
0.2% polyglutamic acid amino-oligosaccharide granule of S8
Polyglutamic acid 0.19%, amino-oligosaccharide 0.01%, adhesive hydroxyethyl cellulose 2%, filler clay are supplied.It presses
More solito formulation method is made.
5% polyglutamic acid amino-oligosaccharide granule of S9
Polyglutamic acid 1%, amino-oligosaccharide 4%, adhesive polyvinyl alcohol 5%, filler diatomite are supplied.According to conventional system
Agent method is made.
14% polyglutamic acid amino-oligosaccharide aqueous suspension agent of S10
Polyglutamic acid 1%, amino-oligosaccharide 13%, dispersing agent xylonic acid sulfoacid calcium 1%, wetting agent dodecyl benzene sulfonic acid
Sodium 0.5%, thickener xanthan gum 1%, thixotropic agent white carbon black 0.5%, antifreeze glycol 1%, preservative paraformaldehyde
0.5%, deionized water is supplied.It is made according to conventional preparation method.
21% polyglutamic acid amino-oligosaccharide aqueous suspension agent of S11
Polyglutamic acid 20%, amino-oligosaccharide 1%, the formaldehyde condensation products 2% of dispersing agent alkyl sodium naphthalene sulfonate, wetting agent amber
Amber sodium dioctyl sulfo 1.5%, thixotropic agent white carbon black 1.5%, antifreezing agent propylene glycol 1%, is prevented thickener aluminium-magnesium silicate 1%
Rotten agent Kathon CG 0.5%, deionized water are supplied.It is made according to conventional preparation method.
30% polyglutamic acid amino-oligosaccharide granule aqueous suspension agent of S12
The wooden disulfonate acid 3% of polyglutamic acid 5%, amino-oligosaccharide 25%, dispersing agent, wetting agent dodecyl benzene sulfonic acid
Sodium 2%, thickener xanthan gum 1.5%, thixotropic agent white carbon black 2.5%, antifreezing agent glycerine 1.5%, preservative paraformaldehyde
1%, deionized water is supplied.It is made according to conventional preparation method.
Polyglutamic acid eugenol
30% polyglutamic acid eugenol wettable powder of S1
Polyglutamic acid 12%, eugenol 18%, dispersing agent sodium lignin sulfonate 4%, wetting agent dodecyl benzene sulfonic acid
Sodium 3%, filler clay are supplied.It is made according to conventional preparation method.
24% polyglutamic acid eugenol wettable powder of S2
Polyglutamic acid 2%, dispersing agent sodium polycarboxylate 3%, wetting agent lauryl sodium sulfate 2%, is filled out eugenol 22%
Material attapulgite is supplied.It is made according to conventional preparation method.
18% polyglutamic acid eugenol wettable powder of S3
Polyglutamic acid 17%, eugenol 1%, dispersing agent Negel 2%, wetting agent neopelex
1.5%, filler bentonite is supplied.It is made according to conventional preparation method.
37% polyglutamic acid eugenol water dispersible granules of S4
Polyglutamic acid 17%, eugenol 20%, dispersing agent sodium naphthalene sulfonate 4.5%, wetting agent lauryl sodium sulfate
2.5%, disintegrating agent ammonium sulfate 5.5%, binder starch 4%, filler diatomite are supplied.It is made according to conventional preparation method.
29% polyglutamic acid eugenol water dispersible granules of S5
The wooden disulfonate acid 3.5% of polyglutamic acid 2%, eugenol 27%, dispersing agent, wetting agent lauryl sodium sulfate
1.5%, disintegrating agent sodium sulphate 4.5%, binder methylcellulose 2.5%, filler clay are supplied.According to conventional preparation method system
.
20% polyglutamic acid eugenol water dispersible granules of S6
The wooden disulfonate acid 2.5% of polyglutamic acid 19%, eugenol 1%, dispersing agent, wetting agent lauryl sodium sulfate
1%, disintegrating agent ammonium sulfate 3.5%, binder glucose 1.5%, filler attapulgite are supplied.According to conventional formulation method system
.
1% polyglutamic acid eugenol granule of S7
Polyglutamic acid 0.35%, eugenol 0.65%, adhesive dextrin 4%, filler attapulgite are supplied.According to routine
Formulation method is made.
5% polyglutamic acid eugenol granule of S8
Polyglutamic acid 4.75%, eugenol 0.25%, adhesive hydroxyethyl cellulose 6%, filler clay are supplied.According to
Conventional preparation method is made.
10% polyglutamic acid eugenol granule of S9
Polyglutamic acid 1.5%, eugenol 8.5%, adhesive polyvinyl alcohol 8%, filler diatomite are supplied.According to routine
Formulation method is made.
16% polyglutamic acid eugenol aqueous suspension agent of S10
Polyglutamic acid 1%, eugenol 15%, dispersing agent xylonic acid sulfoacid calcium 1.5%, wetting agent dodecyl benzene sulfonic acid
Sodium 0.5%, thickener xanthan gum 0.5%, thixotropic agent white carbon black 1%, antifreeze glycol 1%, preservative paraformaldehyde
0.5%, deionized water is supplied.
27% polyglutamic acid eugenol aqueous suspension agent of S11
Polyglutamic acid 25.5%, eugenol 1.5%, the formaldehyde condensation products 2.5% of dispersing agent alkyl sodium naphthalene sulfonate, wetting
Agent dioctyl sodium sulfosuccinate 1.5%, thickener aluminium-magnesium silicate 1.5%, thixotropic agent white carbon black 2%, antifreezing agent propylene glycol
1.5%, Preservative Kathon CG(Methylchloroisothiazolinone/Methylisothiazolinone combination) 1%, deionized water are supplied.It is made according to conventional preparation method.
32% polyglutamic acid eugenol aqueous suspension agent of S12
The wooden disulfonate acid 3.5% of polyglutamic acid 12%, eugenol 20%, dispersing agent, wetting agent detergent alkylate sulphur
Sour sodium 2.5%, thickener xanthan gum 2%, thixotropic agent white carbon black 2.5%, antifreezing agent glycerine 1.5%, preservative poly first
Aldehyde 1%, deionized water are supplied.Conventionally it is made.
Polyglutamic acid humic acid copper
13% polyglutamic acid humic acid copper wettable powder of S1
Polyglutamic acid 3%, humic acid copper 10%, dispersing agent sodium lignin sulfonate 1.5%, wetting agent dodecyl benzene sulfonic acid
Sodium 1%, filler attapulgite are supplied.It is made according to conventional preparation method.
21% polyglutamic acid humic acid copper wettable powder of S2
Polyglutamic acid 20%, humic acid copper 1%, dispersing agent sodium polycarboxylate 2%, wetting agent lauryl sodium sulfate 1.5%,
Filler bentonite is supplied.It is made according to long formulation method.
28% polyglutamic acid humic acid copper wettable powder of S3
Polyglutamic acid 2%, humic acid copper 26%, dispersing agent Negel 3%, wetting agent neopelex
2%, filler clay is supplied.It is made according to conventional preparation method.
40% polyglutamic acid humic acid copper water dispersion granula of S4
Polyglutamic acid 15%, humic acid copper 25%, dispersing agent sodium naphthalene sulfonate 4%, wetting agent lauryl sodium sulfate 3%,
Disintegrating agent ammonium sulfate 6%, binder starch 4%, filler diatomite are supplied.It is made according to conventional preparation method.
31% polyglutamic acid humic acid copper water dispersion granula of S5
The wooden disulfonate acid 3.5% of polyglutamic acid 29.5%, humic acid copper 1.5%, dispersing agent, wetting agent dodecyl sulphur
Sour sodium 1.5%, disintegrating agent sodium sulphate 5%, binder methylcellulose 2%, filler clay are supplied.According to conventional preparation method system
.
25% polyglutamic acid humic acid copper water dispersion granula of S6
The wooden disulfonate acid 2% of polyglutamic acid 2%, humic acid copper 23%, dispersing agent, wetting agent lauryl sodium sulfate
1%, disintegrating agent ammonium sulfate 3.5%, binder glucose 1%, filler attapulgite are supplied.It is made according to conventional preparation method.
12% polyglutamic acid humic acid copper granule of S7
Polyglutamic acid 11.5%, humic acid copper 0.5%, adhesive dextrin 8%, filler attapulgite are supplied.According to routine
Formulation method is made.
0.5% polyglutamic acid humic acid copper granule of S8
Polyglutamic acid 0.1%, humic acid copper 0.4%, adhesive hydroxyethyl cellulose 3%, filler clay are supplied.According to normal
Regulation agent method is made.
7% polyglutamic acid humic acid copper granule of S9
Polyglutamic acid 0.5%, humic acid copper 6.5%, adhesive polyvinyl alcohol 5%, filler diatomite are supplied.According to routine
Formulation method is made.
13% polyglutamic acid humic acid copper water suspending agent of S10
Polyglutamic acid 1%, humic acid copper 12%, dispersing agent xylonic acid sulfoacid calcium 1%, wetting agent neopelex
0.5%, thickener xanthan gum 1%, thixotropic agent white carbon black 1.5%, antifreeze glycol 1%, preservative paraformaldehyde
0.5%, deionized water is supplied.It is made according to conventional preparation method.
27% polyglutamic acid humic acid copper water suspending agent of S11
Polyglutamic acid 25.5%, humic acid copper 1.5%, dispersing agent alkyl sodium naphthalene sulfonate formaldehyde condensation products 2%, wetting agent
Dioctyl sodium sulfosuccinate 1%, thixotropic agent white carbon black 2%, antifreezing agent propylene glycol 1.5%, is prevented thickener aluminium-magnesium silicate 1%
Rotten agent Kathon CG 1%, deionized water are supplied.It is made according to conventional preparation method.
32% polyglutamic acid humic acid copper water suspending agent of S12
The wooden disulfonate acid 3% of polyglutamic acid 7%, humic acid copper 25%, dispersing agent, wetting agent neopelex
2.5%, thickener xanthan gum 1.5%, thixotropic agent white carbon black 3%, antifreezing agent glycerine 1.5%, preservative paraformaldehyde
1.5%, deionized water is supplied.It is made according to conventional preparation method.
Polyglutamic acid Brevibacillus laterosporus
31% polyglutamic acid Brevibacillus laterosporus wettable powder of S1
Polyglutamic acid 6%, Brevibacillus laterosporus 25%, dispersing agent sodium lignin sulfonate 4%, wetting agent detergent alkylate
Sodium sulfonate 3%, filler clay are supplied.It is made according to conventional preparation method.
17% polyglutamic acid Brevibacillus laterosporus wettable powder of S2
Polyglutamic acid 16.2%, Brevibacillus laterosporus 0.8%, dispersing agent sodium polycarboxylate 3%, wetting agent dodecyl sulphur
Sour sodium 2%, filler attapulgite are supplied.It is made according to conventional preparation method.
26% polyglutamic acid Brevibacillus laterosporus wettable powder of S3
Polyglutamic acid 2%, Brevibacillus laterosporus 24%, dispersing agent Negel 3.5%, wetting agent dodecyl
Benzene sulfonic acid sodium salt 2.5%, filler bentonite are supplied.It is made according to conventional preparation method.
39% polyglutamic acid Brevibacillus laterosporus water dispersible granules of S4
Polyglutamic acid 13%, Brevibacillus laterosporus 26%, dispersing agent sodium naphthalene sulfonate 5%, wetting agent dodecyl sulphate
Sodium 3%, disintegrating agent ammonium sulfate 6%, binder starch 4%, filler diatomite are supplied.It is made according to conventional preparation method.
31% polyglutamic acid Brevibacillus laterosporus water dispersible granules of S5
The wooden disulfonate acid 3% of polyglutamic acid 2%, Brevibacillus laterosporus 29%, dispersing agent, wetting agent dodecyl sulphur
Sour sodium 1.5%, disintegrating agent sodium sulphate 4%, binder methylcellulose 2%, filler clay are supplied.According to conventional preparation method system
.
21% polyglutamic acid Brevibacillus laterosporus water dispersible granules of S6
The wooden disulfonate acid 2% of polyglutamic acid 20%, Brevibacillus laterosporus 1%, dispersing agent, wetting agent dodecyl sulphur
Sour sodium 0.5%, disintegrating agent ammonium sulfate 2.5%, binder glucose 1%, filler attapulgite are supplied.According to conventional preparation method
It is made.
11% polyglutamic acid Brevibacillus laterosporus granule of S7
Polyglutamic acid 3%, Brevibacillus laterosporus 8%, adhesive dextrin 7%, filler attapulgite are supplied.According to routine
Formulation method is made.
0.2% polyglutamic acid Brevibacillus laterosporus granule of S8
Polyglutamic acid 0.19%, Brevibacillus laterosporus 0.01%, adhesive hydroxyethyl cellulose 1.5%, filler clay
It supplies.It is made according to conventional preparation method.
6% polyglutamic acid Brevibacillus laterosporus granule of S9
Polyglutamic acid 0.4%, Brevibacillus laterosporus 5.6%, adhesive polyvinyl alcohol 4%, filler diatomite are supplied.It presses
More solito formulation method is made.
17% polyglutamic acid Brevibacillus laterosporus aqueous suspension agent of S10
Polyglutamic acid 2%, Brevibacillus laterosporus 15%, dispersing agent xylonic acid sulfoacid calcium 1%, wetting agent detergent alkylate
Sodium sulfonate 0.5%, thickener xanthan gum 1.5%, thixotropic agent white carbon black 1%, antifreeze glycol 1%, preservative poly first
Aldehyde 1%, deionized water are supplied.It is made according to formulation method.
29% polyglutamic acid Brevibacillus laterosporus aqueous suspension agent of S11
Polyglutamic acid 9%, Brevibacillus laterosporus 20%, the formaldehyde condensation products 1% of dispersing agent alkyl sodium naphthalene sulfonate, wetting
Agent dioctyl sodium sulfosuccinate 1%, thickener aluminium-magnesium silicate 2%, thixotropic agent white carbon black 1.5%, antifreezing agent propylene glycol
1.5%, Preservative Kathon CG(Methylchloroisothiazolinone/Methylisothiazolinone combination) 1.5%, deionized water are supplied.It is made according to conventional preparation method.
32.5% polyglutamic acid Brevibacillus laterosporus aqueous suspension agent of S12
The wooden disulfonate acid 3% of polyglutamic acid 31%, Brevibacillus laterosporus 1.5%, dispersing agent, wetting agent dodecyl
Benzene sulfonic acid sodium salt 2.5%, thickener xanthan gum 2.5%, thixotropic agent white carbon black 2%, antifreezing agent glycerine 2%, preservative poly
Formaldehyde 1.5%, deionized water are supplied.It is made according to conventional preparation method.
Polyglutamic acid bacillus subtilis
31% polyglutamic acid bacillus subtilis wettable powder of S1
Polyglutamic acid 3%, bacillus subtilis 28%, dispersing agent sodium lignin sulfonate 5%, neopelex
4%, filler clay is supplied.It is made according to the method for conventional formulation.
27% polyglutamic acid bacillus subtilis wettable powder of S2
Polyglutamic acid 7%, dispersing agent sodium polycarboxylate 3.5%, lauryl sodium sulfate 2%, is filled out at bacillus subtilis 20%
Material bentonite is supplied.It is made according to the method for conventional formulation.
13% polyglutamic acid bacillus subtilis wettable powder of S3
Polyglutamic acid 12.5%, bacillus subtilis 0.5%, dispersing agent Negel 2%, dodecyl benzene sulfonic acid
Sodium 1.5%, filler attapulgite are supplied.It is made according to the method for conventional formulation.
41% polyglutamic acid bacillus subtilis water dispersible granules of S4
Polyglutamic acid 39%, bacillus subtilis 2%, dispersing agent sodium naphthalene sulfonate 5%, wetting agent lauryl sodium sulfate
3%, disintegrating agent ammonium sulfate 5%, binder starch 3%, filler diatomite are supplied.It is made according to conventional preparation method.
37% polyglutamic acid bacillus subtilis water dispersible granules of S5
The wooden disulfonate acid 3.5% of polyglutamic acid 2%, bacillus subtilis 35%, dispersing agent, wetting agent dodecyl sulphur
Sour sodium 2%, disintegrating agent sodium sulphate 4.5%, binder methylcellulose 2.5%, filler clay are supplied.According to conventional preparation method
It is made.
28% polyglutamic acid bacillus subtilis water dispersible granules of S6
The wooden disulfonate acid 2% of polyglutamic acid 12%, bacillus subtilis 16%, dispersing agent, wetting agent dodecyl sulphur
Sour sodium 1%, disintegrating agent ammonium sulfate 3%, binder glucose 1.5%, filler attapulgite are supplied.According to conventional preparation method system
.
0.16% polyglutamic acid bacillus subtilis granule of S7
Polyglutamic acid 0.06%, bacillus subtilis 0.1%, adhesive dextrin 2%, filler attapulgite are supplied.According to
Conventional preparation method is made.
6% polyglutamic acid bacillus subtilis granule of S8
Polyglutamic acid 0.5%, bacillus subtilis 5.5%, adhesive hydroxyethyl cellulose 4%, filler clay are supplied.It presses
More solito formulation method is made.
9% polyglutamic acid bacillus subtilis granule of S9
Polyglutamic acid 8.5%, bacillus subtilis 0.5%, adhesive polyvinyl alcohol 7%, filler diatomite are supplied.According to
Conventional preparation method is made.
17% polyglutamic acid bacillus subtilis aqueous suspension agent of S10
Polyglutamic acid 7%, bacillus subtilis 10%, dispersing agent xylonic acid sulfoacid calcium 1%, wetting agent detergent alkylate sulphur
Sour sodium 0.5%, thickener xanthan gum 0.5%, thixotropic agent white carbon black 1%, antifreeze glycol 1%, preservative paraformaldehyde
0.5%, deionized water is supplied.It is made according to conventional preparation method.
22% polyglutamic acid bacillus subtilis aqueous suspension agent of S11
Polyglutamic acid 2%, bacillus subtilis 20%, the formaldehyde condensation products 1.5% of dispersing agent alkyl sodium naphthalene sulfonate, wetting
Agent dioctyl sodium sulfosuccinate 1%, thickener aluminium-magnesium silicate 1%, thixotropic agent white carbon black 1.5%, antifreezing agent propylene glycol
1.5%, Preservative Kathon CG(Methylchloroisothiazolinone/Methylisothiazolinone combination) 1%, deionized water are supplied.It is made according to conventional preparation method.
33% polyglutamic acid bacillus subtilis aqueous suspension agent of S12
The wooden disulfonate acid 4% of polyglutamic acid 31.5%, bacillus subtilis 1.5%, dispersing agent, wetting agent dodecyl
Benzene sulfonic acid sodium salt 2.5%, thickener xanthan gum 1.5%, thixotropic agent white carbon black 2%, antifreezing agent glycerine 1.5%, preservative are more
Polyformaldehyde 1%, deionized water are supplied.It is made according to conventional preparation method.
Polyglutamic acid waxy Bacillus
37% polyglutamic acid waxy Bacillus wettable powder of S1
Polyglutamic acid 35%, waxy Bacillus 2%, dispersing agent sodium lignin sulfonate 5%, wetting agent detergent alkylate sulphur
Sour sodium 4%, filler clay are supplied.It is made according to conventional preparation method.
24% polyglutamic acid waxy Bacillus wettable powder of S2
Polyglutamic acid 3%, waxy Bacillus 21%, dispersing agent sodium polycarboxylate 3%, wetting agent lauryl sodium sulfate
2%, attapulgite is supplied.It is made according to conventional preparation method.
15% polyglutamic acid waxy Bacillus wettable powder of S3
Polyglutamic acid 5%, waxy Bacillus 10%, dispersing agent Negel 2%, wetting agent detergent alkylate sulphur
Sour sodium 1.5%, bentonite are supplied.It is made according to conventional preparation method.
43% polyglutamic acid waxy Bacillus water dispersible granules of S4
Polyglutamic acid 3%, waxy Bacillus 40%, dispersing agent sodium naphthalene sulfonate 5%, wetting agent lauryl sodium sulfate
3.5%, disintegrating agent ammonium sulfate 5%, binder starch 3%, filler diatomite are supplied.It is made according to conventional preparation method.
36% polyglutamic acid waxy Bacillus water dispersible granules of S5
The wooden disulfonate acid 3.5% of polyglutamic acid 34%, waxy Bacillus 2%, dispersing agent, wetting agent dodecyl sulphur
Sour sodium 1.5%, disintegrating agent sodium sulphate 4.5%, binder methylcellulose 2.5%, filler clay are supplied.According to conventional formulation side
Method is made.
21% polyglutamic acid waxy Bacillus water dispersible granules of S6
The wooden disulfonate acid 2% of polyglutamic acid 6%, waxy Bacillus 15%, dispersing agent, wetting agent dodecyl sulphate
Sodium 1%, disintegrating agent ammonium sulfate 3%, binder glucose 1.5%, filler attapulgite are supplied.According to conventional preparation method system
.
0.3% polyglutamic acid waxy Bacillus granule of S7
Polyglutamic acid 0.1%, waxy Bacillus 0.2%, adhesive dextrin 1.5%, filler attapulgite are supplied.According to
Conventional preparation method is made.
4% polyglutamic acid waxy Bacillus granule of S8
Polyglutamic acid 3.8%, waxy Bacillus 0.2%, adhesive hydroxyethyl cellulose 3.5%, filler clay are supplied.
It is made according to conventional preparation method.
13% polyglutamic acid waxy Bacillus granule of S9
Polyglutamic acid 2%, waxy Bacillus 11%, adhesive polyvinyl alcohol 9%, filler diatomite are supplied.According to normal
Regulation agent method is made.
13% polyglutamic acid waxy Bacillus aqueous suspension agent of S10
Polyglutamic acid 5%, waxy Bacillus 8%, dispersing agent xylonic acid sulfoacid calcium 1%, wetting agent detergent alkylate sulphur
Sour sodium 0.5%, thickener xanthan gum 0.5%, thixotropic agent white carbon black 1%, antifreeze glycol 1%, preservative paraformaldehyde
0.5%, deionized water is supplied.It is made according to conventional preparation method.
24% polyglutamic acid waxy Bacillus aqueous suspension agent of S11
Polyglutamic acid 3%, waxy Bacillus 21%, the formaldehyde condensation products 1.5% of dispersing agent alkyl sodium naphthalene sulfonate, wetting
Agent dioctyl sodium sulfosuccinate 1%, thickener aluminium-magnesium silicate 1%, thixotropic agent white carbon black 1.5%, antifreezing agent propylene glycol
1.5%, Preservative Kathon CG(Methylchloroisothiazolinone/Methylisothiazolinone combination) 1%, deionized water are supplied.It is made according to conventional preparation method.
38% polyglutamic acid waxy Bacillus aqueous suspension agent of S12
The wooden disulfonate acid 4% of polyglutamic acid 36%, waxy Bacillus 2%, dispersing agent, wetting agent detergent alkylate sulphur
Sour sodium 2%, thickener xanthan gum 2%, thixotropic agent white carbon black 2.5%, antifreezing agent glycerine 2.5%, preservative paraformaldehyde
1.5%, deionized water is supplied.It is made according to conventional preparation method.
Polyglutamic acid bacillus licheniformis
37% polyglutamic acid bacillus licheniformis wettable powder of S1
Polyglutamic acid 35%, bacillus licheniformis 2%, dispersing agent sodium lignin sulfonate 5%, wetting agent detergent alkylate sulphur
Sour sodium 4%, filler clay are supplied.It is made according to conventional preparation method.
23% polyglutamic acid bacillus licheniformis wettable powder of S2
Polyglutamic acid 7%, bacillus licheniformis 16%, dispersing agent sodium polycarboxylate 3%, wetting agent lauryl sodium sulfate
2%, filler attapulgite is supplied.
19% polyglutamic acid bacillus licheniformis wettable powder of S3
Polyglutamic acid 1%, bacillus licheniformis 18%, dispersing agent Negel 2%, wetting agent detergent alkylate sulphur
Sour sodium 1.5%, filler bentonite are supplied.It is made according to conventional preparation method.
42% polyglutamic acid bacillus licheniformis water dispersible granules of S4
Polyglutamic acid 40%, bacillus licheniformis 2%, dispersing agent sodium naphthalene sulfonate 6%, wetting agent lauryl sodium sulfate
3%, disintegrating agent ammonium sulfate 5%, binder starch 3%, filler diatomite are supplied.It is made according to conventional preparation method.
36% polyglutamic acid bacillus licheniformis water dispersible granules of S5
The wooden disulfonate acid 3.5% of polyglutamic acid 4%, bacillus licheniformis 32%, dispersing agent, wetting agent dodecyl sulphur
Sour sodium 1.5%, disintegrating agent sodium sulphate 4.5%, binder methylcellulose 2.5%, filler clay are supplied.According to conventional formulation side
Method is made.
21% polyglutamic acid bacillus licheniformis water dispersible granules of S6
The wooden disulfonate acid 2% of polyglutamic acid 7%, bacillus licheniformis 14%, dispersing agent, wetting agent dodecyl sulphate
Sodium 1%, disintegrating agent ammonium sulfate 3%, binder glucose 1.5%, filler attapulgite are supplied.According to conventional formulation method system
Obtain water dispersible granules product of the invention.It is made according to conventional preparation method.
0.2% polyglutamic acid granular bacillus licheniformis agent of S7
Polyglutamic acid 0.08%, bacillus licheniformis 0.12%, adhesive dextrin 2.5%, filler attapulgite are supplied.It presses
More solito formulation method is made.
7.5% polyglutamic acid granular bacillus licheniformis agent of S8
Polyglutamic acid 7.1%, bacillus licheniformis 0.4%, adhesive hydroxyethyl cellulose 6%, filler clay are supplied.It presses
More solito formulation method is made.
14% polyglutamic acid granular bacillus licheniformis agent of S9
Polyglutamic acid 5%, bacillus licheniformis 9%, adhesive polyvinyl alcohol 8%, filler diatomite are supplied.According to routine
Formulation method is made.16% polyglutamic acid bacillus licheniformis aqueous suspension agent of S10
Polyglutamic acid 6%, bacillus licheniformis 10%, dispersing agent xylonic acid sulfoacid calcium 1%, wetting agent detergent alkylate sulphur
Sour sodium 0.5%, thickener xanthan gum 0.5%, thixotropic agent white carbon black 1%, antifreeze glycol 1%, preservative paraformaldehyde
0.5%, deionized water is supplied.It is made according to conventional preparation method.
29% polyglutamic acid bacillus licheniformis aqueous suspension agent of S11
Polyglutamic acid 27%, bacillus licheniformis 2%, dispersing agent alkyl sodium naphthalene sulfonate formaldehyde condensation products 2%, wetting agent
Dioctyl sodium sulfosuccinate 1%, thixotropic agent white carbon black 2%, antifreezing agent propylene glycol 1.5%, is prevented thickener aluminium-magnesium silicate 1%
Rotten agent Kathon CG 1%, deionized water are supplied.It is made according to conventional preparation method.
31% polyglutamic acid bacillus licheniformis aqueous suspension agent of S12
The wooden disulfonate acid 3.5% of polyglutamic acid 2%, bacillus licheniformis 29%, dispersing agent, wetting agent detergent alkylate
Sodium sulfonate 2%, thickener xanthan gum 2%, thixotropic agent white carbon black 2.5%, antifreezing agent glycerine 2.5%, preservative poly first
Aldehyde 1.5%, deionized water are supplied.It is made according to conventional preparation method.
Comparative example
3% polyglutamic acid aqua of D1 is made according to conventional preparation method
0.5% amino-oligosaccharide aqueous suspension agent of D2 is made according to conventional preparation method
0.3% eugenol soluble concentrate of D3 is made according to conventional preparation method
30% humic acid copper wettable powder of D4 is made according to conventional preparation method
10,000,000,000 spore of D5/gram Brevibacillus laterosporus wettable powder is made according to conventional preparation method
10,000,000,000 spore of D6/gram bacillus subtilis wettable powder is made according to conventional preparation method
2,000,000,000 spore of D7/gram waxy Bacillus wettable powder is made according to conventional preparation method
8,000,000,000 spores of D8/milliliter bacillus licheniformis aqua is made according to conventional preparation method
Toxicity determination test
Polyglutamic acid and amino-oligosaccharide
Subjects are rice blast.Test method is with reference to " NY/T 1156.6-2006 farm-chemical indoor determination examination
Test the 6th part of criterion fungicide: mixed synergy measurement " and " NY/T 1156.8-2007 farm-chemical indoor determination examination
Test the 8th part of criterion fungicide: prevention and treatment rice blast test pot-culture method ".According to survey data, the state of an illness for calculating each processing refers to
Several and control efficiency.By the linear regression analysis between preventive effect probability value and the logarithm of series of concentrations, each medicament is found out
EC50Value.The co-toxicity coefficient (CTC) of mixture is calculated with Sun Yunpei's Method, and medicament is evaluated to the activity of germ with this.CTC≥120
Show as synergistic effect；CTC≤80 show as antagonism；80 < CTC < 120 show as summation action.
1 polyglutamic acid of table is with amino-oligosacchride promotor composition to the indoor toxicity test of rice blast
| Chemicals treatment | EC50(mg/L) | ATI | TTI | CTC |
| Polyglutamic acid (A) | 0.13 | 235.96 | _ | _ |
| Amino-oligosaccharide (B) | 0.58 | 100.00 | _ | _ |
| A:B=20:1 | 0.19 | 219.75 | 175.64 | 125.11 |
| A:B=15:1 | 0.24 | 211.43 | 169.72 | 124.58 |
| A:B=10:1 | 0.27 | 204.68 | 164.38 | 124.52 |
| A:B=5:1 | 0.31 | 198.57 | 158.51 | 125.27 |
| A:B=1:1 | 0.37 | 191.29 | 152.39 | 125.53 |
| A:B=1:5 | 0.39 | 185.12 | 146.67 | 126.22 |
| A:B=1:10 | 0.42 | 181.45 | 140.85 | 128.82 |
| A:B=1:15 | 0.46 | 176.63 | 136.49 | 129.41 |
| A:B=1:20 | 0.49 | 171.09 | 132.91 | 128.73 |
Table 1 shows that polyglutamic acid is compounded with amino-oligosaccharide shows as apparent synergistic effect to rice blast, especially
It is 1:15 when ratio, and synergistic effect is the most significant.Applicant's test simultaneously is it has also been found that polyglutamic acid and amino-oligosaccharide
When proportion is within the scope of 20:1-1:20, to cucumber fusarium axysporum, watermelon blight, tomato late blight, wheat scab, tobacco disease
Viral disease, pear scab, there is synergistic effect.
Polyglutamic acid and eugenol
Subjects are tomato late blight.Test method is referring to " NY/T 1156.6-2006 farm-chemical indoor determination examination
Test the 6th part of criterion fungicide: mixed synergy measurement " and " NY/T 1156.13-2008 farm-chemical indoor determination examination
It tests the 13rd part of criterion fungicide: inhibiting late disease bacteria pilot blade method ".According to survey data, the state of an illness for calculating each processing refers to
Several and control efficiency.By the linear regression analysis between preventive effect probability value and the logarithm of series of concentrations, each medicament is found out
EC50Value.The co-toxicity coefficient (CTC) of mixture is calculated with Sun Yunpei's Method, and medicament is evaluated to the activity of germ with this.CTC≥120
Show as synergistic effect；CTC≤80 show as antagonism；80 < CTC < 120 show as summation action.
2 polyglutamic acid of table and eugenol combine the indoor toxicity test to tomato late blight
Table 2 is as can be seen that polyglutamic acid and eugenol complex composition show as synergistic effect to tomato late blight；Its
The proportion of middle polyglutamic acid and eugenol is 15:1, most significant to the synergistic effect of tomato late blight.Applicant's test simultaneously
It has also been found that when the proportion of polyglutamic acid and eugenol is within the scope of 20:1-1:20, to graw mold of tomato, tomato downy mildew, kind
Eggplant virosis, downy mildew of garpe, there is synergistic effect.
Polyglutamic acid and humic acid copper
Subjects: apple tree canker.Test method reference test method refers to " NY/T 1156.6-2006 pesticide room
Interior the 6th part of bioassay test criterion fungicide: mixed synergy measurement " and " NY/T 1156.5-2006 pesticide room
Interior bioassay test criterion fungicide part 2: inhibit disease fungus mycelia growth test Plating ".
According to survey data, the disease index and control efficiency of each processing are calculated.Pass through preventive effect probability value and series of concentrations
Logarithm between linear regression analysis, find out the EC of each medicament50Value.
The co-toxicity coefficient (CTC) of mixture is calculated with Sun Yunpei's Method, and medicament is evaluated to the activity of germ with this.CTC≥
120 show as synergistic effect；CTC≤80 show as antagonism；80 < CTC < 120 show as summation action.
3 polyglutamic acid of table is with humic acid copper composition to the indoor toxicity test of apple tree canker
Table 3 is as can be seen that polyglutamic acid compounds with humic acid copper and shows as synergistic effect to apple tree canker；Wherein gather
The proportion of glutamic acid and humic acid copper is 1:1, shows as synergistic effect to apple tree canker.Applicant's test simultaneously also found
When the proportion of polyglutamic acid and humic acid copper is within the scope of 20:1-1:20, to mandarin tree foot rot, angular leaf spot of cucumber, there is increasing
Effect effect.
Polyglutamic acid and Brevibacillus laterosporus
Subjects: capsicum epidemic disease.Test method is referring to " test of NY/T 1156.6-2006 farm-chemical indoor determination is quasi-
Then the 6th part of fungicide: mixed synergy measurement " and " NY/T1156.2-2006 farm-chemical indoor determination test rule
Fungicide part 2: inhibit disease fungus mycelia growth test Plating ".
According to survey data, the disease index and control efficiency of each processing are calculated.Pass through preventive effect probability value and series of concentrations
Logarithm between linear regression analysis, find out the EC50 value of each medicament.
The co-toxicity coefficient (CTC) of mixture is calculated with Sun Yunpei's Method, and medicament is evaluated to the activity of germ with this.CTC≥
120 show as synergistic effect；CTC≤80 show as antagonism；80 < CTC < 120 show as summation action.
4 polyglutamic acid of table is with Brevibacillus laterosporus composition to the indoor toxicity test of capsicum epidemic disease
| Chemicals treatment | EC50(mg/L) | ATI | TTI | CTC |
| Polyglutamic acid (A) | 0.76 | 313.29 | _ | _ |
| Brevibacillus laterosporus (B) | 4.38 | 100.00 | _ | _ |
| A:B=1:20 | 0.99 | 264.37 | 200.39 | 131.93 |
| A:B=15:1 | 1.43 | 258.81 | 195.65 | 132.28 |
| A:B=10:1 | 1.75 | 240.93 | 181.28 | 132.90 |
| A:B=5:1 | 2.12 | 234.59 | 178.43 | 131.47 |
| A:B=1:1 | 2.53 | 226.74 | 164.56 | 137.79 |
| A:B=1:5 | 2.98 | 213.97 | 157.49 | 135.86 |
| A:B=1:10 | 3.42 | 201.59 | 143.67 | 140.31 |
| A:B=1:15 | 3.68 | 196.48 | 131.82 | 149.05 |
| A:B=1:20 | 4.09 | 181.27 | 126.48 | 143.32 |
Table 4 can be seen that polyglutamic acid and compound in the mass ratio range of 20:1-1:20 with Brevibacillus laterosporus,
CTC value > 120, show as synergistic effect to capsicum epidemic disease；Wherein the proportion of polyglutamic acid and Brevibacillus laterosporus is 1:
15, synergistic effect is shown as to capsicum epidemic disease.Applicant's test simultaneously is it has also been found that polyglutamic acid and Brevibacillus laterosporus are matched
When than within the scope of 20:1-1:20, to watermelon blight, wheat scab, grape grey mould, Strawberry anthracnose, there is synergy
Effect.
Polyglutamic acid and bacillus subtilis
Subjects: graw mold of tomato.Test method is referring to " NY/T 1156.6-2006 farm-chemical indoor determination test
The 6th part of criterion fungicide: mixed synergy measurement " and " NY/T1156.10-2008 farm-chemical indoor determination test
The 11st part of criterion fungicide: prevention and treatment gray mold tests pot-culture method ".
According to survey data, the disease index and control efficiency of each processing are calculated.Pass through preventive effect probability value and series of concentrations
Logarithm between linear regression analysis, find out the EC of each medicament50Value.
The co-toxicity coefficient (CTC) of mixture is calculated with Sun Yunpei's Method, and medicament is evaluated to the activity of germ with this.CTC≥
120 show as synergistic effect；CTC≤80 show as antagonism；80 < CTC < 120 show as summation action.
5 polyglutamic acid of table is with bacillus subtilis bacteria composition to the indoor toxicity test of graw mold of tomato
| Chemicals treatment | EC50(mg/L) | ATI | TTI | CTC |
| Polyglutamic acid (A) | 0.0019 | 289.75 | - | - |
| Bacillus subtilis (B) | 0.0312 | 100.00 | - | - |
| A:B=20:1 | 0.0028 | 276.13 | 203.14 | 135.93 |
| A:B=15:1 | 0.0043 | 257.92 | 189.75 | 135.93 |
| A:B=10:1 | 0.0065 | 238.71 | 172.38 | 138.48 |
| A:B=5:1 | 0.0097 | 219.68 | 163.56 | 134.31 |
| A:B=1:1 | 0.0125 | 192.36 | 154.92 | 124.17 |
| A:B=1:5 | 0.0168 | 178.54 | 143.27 | 124.62 |
| A:B=1:10 | 0.0204 | 176.72 | 130.98 | 134.92 |
| A:B=1:15 | 0.0257 | 165.39 | 123.47 | 133.95 |
| A:B=1:20 | 0.0284 | 153.87 | 119.26 | 129.02 |
Table 5 can be seen that polyglutamic acid compounds in the mass ratio range of 20:1-1:20 with bacillus subtilis, CTC value
> 120 show as synergistic effect to graw mold of tomato；Wherein the proportion of polyglutamic acid and bacillus subtilis is 10:1, right
Graw mold of tomato shows as synergistic effect.Applicant's test simultaneously is it has also been found that the proportion of polyglutamic acid and Brevibacillus laterosporus is
When within the scope of 20:1-1:20, to citrus bacterial canker disease, gray mold of cucumber, rice blast, rice green smut, bacterial blight of rice,
Powdery mildew of cucumber, powdery mildew of strawberry, bacterial wilt of tomato, tobacco bacterial wilt, soft rot of cabbage, the late blight of potato, cotton yellow wither
Disease, capsicum wilt, there is synergistic effect.
Polyglutamic acid and waxy Bacillus
Subjects are rice sheath blight disease.Test method is with reference to " NY/T 1156.6-2006 farm-chemical indoor determination examination
Test the 6th part of criterion fungicide: mixed synergy measurement " and " NY/T 1156.5-2006 farm-chemical indoor determination examination
It tests the 5th part of criterion fungicide: inhibiting Rhizoctonia solani Kuhn test Broad Bean Leaves method ".
According to survey data, the disease index and control efficiency of each processing are calculated.Pass through preventive effect probability value and series of concentrations
Logarithm between linear regression analysis, find out the EC of each medicament50Value.
The co-toxicity coefficient (CTC) of mixture is calculated with Sun Yunpei's Method, and medicament is evaluated to the activity of germ with this.CTC≥
120 show as synergistic effect；CTC≤80 show as antagonism；80 < CTC < 120 show as summation action.
6 polyglutamic acid of table is with waxy Bacillus composition to the indoor toxicity test of rice sheath blight disease
| Chemicals treatment | EC50(mg/L) | ATI | TTI | CTC |
| Polyglutamic acid (A) | 3.89 | 423.98 | _ | _ |
| Waxy Bacillus (B) | 15.03 | 100.00 | _ | _ |
| A:B=20:1 | 4.25 | 409.75 | 332.49 | 123.24 |
| A:B=15:1 | 5.62 | 378.19 | 314.52 | 120.24 |
| A:B=10:1 | 6.79 | 334.94 | 279.08 | 120.02 |
| A:B=5:1 | 7.57 | 295.35 | 241.67 | 122.21 |
| A:B=1:1 | 8.46 | 257.61 | 214.35 | 120.18 |
| A:B=1:5 | 9.12 | 226.47 | 182.76 | 123.92 |
| A:B=1:10 | 10.43 | 191.29 | 156.81 | 121.99 |
| A:B=1:15 | 11.98 | 164.85 | 131.27 | 125.58 |
| A:B=1:20 | 14.15 | 139.73 | 113.43 | 123.19 |
Table 6 show, polyglutamic acid is mixed by 20:1-1:20 with waxy Bacillus, to rice sheath blight disease show as it is bright
Aobvious synergistic effect, especially when ratio is 1:15, synergistic effect is the most significant.Applicant's test simultaneously is it has also been found that polyglutamic acid
When proportion with waxy Bacillus is within the scope of 20:1-1:20, to rice green smut, rice blast, eggplant bacterial wilt, grass
Certain kind of berries powdery mildew, there is synergistic effect.
Polyglutamic acid and bacillus licheniformis
Subjects are cucumber downy mildew.Test method is with reference to " NY/T 1156.6-2006 farm-chemical indoor determination examination
Test the 6th part of criterion fungicide: mixed synergy measurement " and " NY/T 1156.7-2006 farm-chemical indoor determination examination
Test the 7th part of criterion fungicide: prevention and treatment cucumber downy mildew test pot-culture method ".
According to survey data, the disease index and control efficiency of each processing are calculated.Pass through preventive effect probability value and series of concentrations
Logarithm between linear regression analysis, find out the EC of each medicament50Value.
The co-toxicity coefficient (CTC) of mixture is calculated with Sun Yunpei's Method, and medicament is evaluated to the activity of germ with this.CTC≥
120 show as synergistic effect；CTC≤80 show as antagonism；80 < CTC < 120 show as summation action.
7 polyglutamic acid of table is with bacillus licheniformis composition to the indoor toxicity test of cucumber downy mildew
Table 7 shows that polyglutamic acid compounds (20:1-1:20) with bacillus licheniformis and shows as increasing to cucumber downy mildew
Effect, especially when ratio is 1:15, synergistic effect is the most significant.Applicant's test simultaneously is it has also been found that polyglutamic acid and lichens gemma
When the proportion of bacillus is 20:1-1:20 range, to take-all, Alternaria alternate, tobacco black shank, rice green smut, west
Cucurbit wilt has synergistic effect.
2) field control effectiveness test
Polyglutamic acid and amino-oligosaccharide
Reagent agent: embodiment S1-12；Comparison medicament: comparative example D1, D2；Subjects: tomato late blight.
Test method: using conventional spraying methods, and period of disease is administered for the first time, is spaced 7d, is administered for the second time.
Investigation and statistical method: investigating state of an illness radix before application, 7d investigation is primary after being administered for the first time, after second is administered
7d, 14d are respectively investigated once.Every random 5 points of samplings of cell, often click two plants, every plant is divided upper, middle and lower to investigate 10 leaves, with every
Lesion area on a piece of leaf accounts for the percentage of entire leaf area to be classified.Stage division:
0 grade: disease-free spot；
1 grade: lesion area accounts for entire 5% or less leaf area；
3 grades: lesion area accounts for entire leaf area 6%-10%；
5 grades: lesion area accounts for entire leaf area 11%-20%；
7 grades: lesion area accounts for entire leaf area 21%-50%；
9 grades: lesion area accounts for entire 50% or more leaf area.
Disease index=[(the disease numbers of sheets at different levels × opposite value of series)/(the investigation number of blade × 9) Σ] × 100
Control efficiency/%=(blank control disease index-chemicals treatment disease index)/blank control disease index ×
100
Table 8 prevents and treats tomato late blight field trial
As can be seen from Table 8, polyglutamic acid is compounded with amino-oligosaccharide, and compared with single dose, which is improved
Bactericidal effect, and preventive effect phase is long；Composition is made of the effective component of different role mechanism, and action site increases, and is conducive to
Overcome and germ drug resistance is delayed to generate.Cucumber fusarium axysporum, watermelon blight, rice blast, gibberella saubinetii can be prevented and treated simultaneously
Disease, tobacco virus, pear scab, control efficiency are also superior to the preventive effect of single dose.Rake is denoted as in test scope of medication
Object is without influence.
Polyglutamic acid and eugenol
Reagent agent: embodiment S1-12；Comparison medicament: comparative example D1, D3；Subjects: tomato late blight.
Test method: using conventional spray-on process, early stage is administered for the first time, is spaced second of 10d and is administered.
Investigation and statistical method: investigating state of an illness radix before application, 7d investigation is primary after being administered for the first time, after second is administered
7d, 14d are respectively investigated once.Every random 5 points of samplings of cell, often click two plants, every plant is divided upper, middle and lower to investigate 10 leaves, with every
Lesion area on a piece of leaf accounts for the percentage of entire leaf area to be classified.Stage division:
0 grade: disease-free spot；
1 grade: lesion area accounts for entire 5% or less leaf area；
3 grades: lesion area accounts for entire leaf area 6%-10%；
5 grades: lesion area accounts for entire leaf area 11%-20%；
7 grades: lesion area accounts for entire leaf area 21%-50%；
9 grades: lesion area accounts for entire 50% or more leaf area.
Disease index=[(the disease numbers of sheets at different levels × opposite value of series)/(the investigation number of blade × 9) Σ] × 100
Control efficiency/%=(blank control disease index-chemicals treatment disease index)/blank control disease index ×
100
Table 9 prevents and treats tomato late blight field trial
As can be seen from Table 9, polyglutamic acid is compounded with eugenol, the sterilization compared with single dose, to tomato late blight
Effect improves, and preventive effect phase is long.Graw mold of tomato, tomato downy mildew, tomato virus disease, grape downy mildew can also be prevented and treated simultaneously
Disease, control efficiency is also superior to the preventive effect of single dose, and preventive effect phase is long.Object is denoted as without influence on rake in test scope of medication.
Polyglutamic acid and humic acid copper
Reagent agent: embodiment S1-12；Comparison medicament: comparative example D1, D4；Subjects: angular leaf spot of cucumber.
Test method: using conventional spray-on process, early stage starts to be administered for the first time, is spaced 10d, is administered for the second time.
Investigation and statistical method: investigating state of an illness radix before application, 7d investigation is primary after being administered for the first time, after second is administered
7d, 14d are respectively investigated once.5 points of every cell diagonal line samplings, 3 plants every, 3 points of protecting field (canopy room) samplings, are adjusted by 5 plants every
Whole blades are looked into, entire blade area percentage classification is accounted for every leaf spot lesion area.Stage division:
0 grade: disease-free spot；
1 grade: lesion area accounts for 5% or less full wafer leaf area；
3 grades: lesion area accounts for the 6%-10% of full wafer leaf area；
5 grades: lesion area accounts for the 11%-20% of full wafer leaf area；
7 grades: lesion area accounts for the 21%-50% of full wafer leaf area；
9 grades: lesion area accounts for 51% or more of full wafer leaf area.
Disease index=[(the disease numbers of sheets at different levels × opposite value of series)/(investigation total leaf number × 9) Σ] × 100
Control efficiency/%=(blank control disease index-chemicals treatment disease index)/blank control disease index ×
100
Table 10 prevents and treats angular leaf spot of cucumber field trial
Table 10 shows that compared with single dose, polyglutamic acid is improved with sterilizing ability of the humic acid copper to angular leaf spot of cucumber, and
Preventive effect phase is long；Apple tree canker, mandarin tree foot rot can also be prevented and treated simultaneously, control efficiency is also superior to the preventive effect of single dose,
And preventive effect phase is long.Object is denoted as without influence on rake in test scope of medication.
Polyglutamic acid and Brevibacillus laterosporus
Reagent agent: embodiment S1-12；Comparison medicament: comparative example D1, D5；Subjects: capsicum epidemic disease.
Test method: using conventional spray-on process, early stage starts to be administered for the first time, is spaced 20d, is administered for the second time.
Investigation and statistical method: investigating state of an illness radix before application, 10d investigation is primary after first time is administered, second of application
10d, 20d are respectively investigated once afterwards.5 points of every cell random searching, every is looked into 5 plants.Stage division: (being classified by symptom type)
0 grade: health is without disease；
1 grade: only leaf, fruit have scab to overground part；
3 grades: the aerial stem of plant, branch have brown rot spot；
5 grades: basal part of stem has brown rot spot；
7 grades: the aerial stem of plant, branch and basal part of stem have brown rot spot, and part branch is withered；
9 grades: complete stool is withered.
Disease index=[(the disease numbers of sheets at different levels × opposite value of series)/(investigation total leaf number × 9) Σ] × 100
Control efficiency/%=(blank control disease index-chemicals treatment disease index)/blank control disease index ×
100
Table 11 prevents and treats capsicum epidemic disease field trial
Table 11 is shown, compared with single dose, polyglutamic acid of the invention and Brevibacillus laterosporus bactericidal composition are to capsicum
Epidemic disease improves bactericidal effect, and preventive effect phase is long；Watermelon blight, wheat scab, grape grey mold can also be prevented and treated simultaneously
Disease, Strawberry anthracnose, control efficiency is also superior to the preventive effect of single dose, and preventive effect phase is long.Rake is denoted as in test scope of medication
Object is without influence.
Polyglutamic acid and bacillus subtilis
Reagent agent: embodiment S1-12；Comparison medicament: comparative example D1, D6；Subjects: powdery mildew of cucumber.
Test method: using conventional spray-on process, early stage starts to be administered for the first time, is spaced 10d, is administered for the second time.
Investigation and statistical method: investigating state of an illness radix before application, 7d investigation is primary after being administered for the first time, after second is administered
7d, 14d are respectively investigated once.Every cell takes at 4 points at random, whole blades of every 2 plants of investigation.Stage division:
0 grade: disease-free spot；
1 grade: lesion area accounts for entire 5% or less leaf area；
3 grades: lesion area accounts for entire leaf area 6%-10%；
5 grades: lesion area accounts for entire leaf area 11%-20%；
7 grades: lesion area accounts for entire leaf area 21%-40%；
9 grades: lesion area accounts for entire 40% or more leaf area.
Disease index=[(the disease numbers of sheets at different levels × opposite value of series)/(investigation total number of sheets × 4) Σ] × 100
Control efficiency/%=(blank control disease index-chemicals treatment disease index)/blank control disease index ×
100
Table 12 prevents and treats powdery mildew of cucumber field trial
Table 12 shows that compared with single dose, which significantly improves the bactericidal effect of powdery mildew of cucumber, and preventive effect
Phase is long；Citrus bacterial canker disease, gray mold of cucumber, graw mold of tomato, rice blast, rice green smut, rice can be also prevented and treated simultaneously
Bacterial leaf-blight, powdery mildew of strawberry, bacterial wilt of tomato, tobacco bacterial wilt, soft rot of cabbage, the late blight of potato, cotton verticillium wilt,
Capsicum wilt, and preventive effect phase is long.Object is denoted as without influence on rake in test scope of medication.
Polyglutamic acid and waxy Bacillus
Reagent agent: embodiment S1-12；Comparison medicament: comparative example D1, D7；Subjects: eggplant bacterial wilt.
Test method: spraying using routine, early stage starts to be administered for the first time, is spaced 10d, second of application, interval
10d, third time are administered.
Investigation and statistical method: 7d is investigated after being administered for the first time, and 7d is investigated after being administered for the second time, 7d tune after third time is administered
It looks into.Every cell diagonal line fixes 5 points of samplings, and every is looked into 5 plants, and record investigates total strain number and diseased plant number.
Diseased plant rate=diseased plant number/investigation total strain number × 100
Control efficiency/%=(blank district diseased plant rate-treatment region diseased plant rate)/blank control diseased plant rate × 100
Table 13 prevents and treats eggplant bacterial wilt field trial
As can be seen from Table 13, compared with single dose, which obviously mentions the bactericidal effect of eggplant bacterial wilt
Height, and preventive effect phase is long；Rice green smut, rice blast, rice sheath blight disease, powdery mildew of strawberry, prevention and treatment effect can be also prevented and treated simultaneously
Fruit is also superior to the preventive effect of single dose, and preventive effect phase is long.Object is denoted as without influence on rake in test scope of medication.
Polyglutamic acid and bacillus licheniformis
Reagent agent: embodiment S1-12；Comparison medicament: comparative example D1, D8；Subjects: cucumber downy mildew.
Test method: using conventional spray-on process, early stage starts to be administered for the first time, is spaced 10d, is administered for the second time.
Investigation and statistical method: investigating state of an illness radix before application, 7d investigation is primary after being administered for the first time, after second is administered
7d, 14d are respectively investigated once.Every cell takes 4 points of investigation at random, and every is looked into two plants, the whole piece leaves of every plant of investigation.Stage division (with
Blade is unit):
0 grade: disease-free spot；
1 grade: lesion area accounts for 5% or less entire leaf area；
3 grades: lesion area accounts for the 6%-10% of entire leaf area；
5 grades: lesion area accounts for the 11%-25% of entire leaf area；
7 grades: lesion area accounts for the 26%-50% of entire leaf area；
9 grades: lesion area accounts for 50% or more of entire leaf area.
Disease index=[(the disease numbers of sheets at different levels × opposite value of series)/(investigation total number of sheets × 4) Σ] × 100
Control efficiency/%=(blank control disease index-chemicals treatment disease index)/blank control disease index ×
100
Table 14 prevents and treats cucumber downy mildew field trial
As can be seen from Table 14, compared with single dose, which obviously mentions the bactericidal effect of cucumber downy mildew
Height, and preventive effect phase is long；It is withered that take-all, Alternaria alternate, tobacco black shank, rice green smut, watermelon can also be prevented and treated simultaneously
It withers disease, control efficiency is also superior to the preventive effect of single dose, and preventive effect phase is long.Object is denoted as without influence on rake in test scope of medication.
It is known that the illustrative embodiments that above-described embodiment uses only for illustrating inventive principle, however it is of the invention
It is not limited only to this, those skilled in the art can make various improvement and change in the case where not departing from real situation of the present invention, these
It improves and change also belongs to protection scope of the present invention.
Claims (5)
1. a kind of bactericidal composition, it is characterised in that: effective component is A and B, and the effective constituents A is polyglutamic acid, effectively
Ingredient B is amino-oligosaccharide, eugenol, humic acid copper, Brevibacillus laterosporus, bacillus subtilis, wax gemma bar
The mass ratio of one of bacterium, bacillus licheniformis, effective constituents A and B are 1:40~40:1, and sum of the two is in bactericidal composition
In mass percentage be 0.1-50%.
2. bactericidal composition according to claim 1, it is characterised in that: the mass ratio of effective constituents A and B be 1:20~
20:1。
3. bactericidal composition described in -2 any one according to claim 1, it is characterised in that: can be prepared into aqueous suspension agent, can
Wet powder, water dispersible granules, granule.
4. bactericidal composition is used to prevent and treat crop disease and promote the purposes of plant growth according to claim 1.
5. bactericidal composition according to claim 4, it is characterised in that: the disease is powdery mildew, angular leaf spot, red mould
Disease, late blight, wilt disease, false smut, rice blast, bacterial wilt, soft rot, banded sclerotial blight, verticillium wilt, balck shank, anthracnose, late blight
Disease, gray mold, downy mildew, bacterial leaf-blight, epidemic disease, virosis, scab, rot disease, foot rot, full rot.
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| CN201810472191.XA CN110495456A (en) | 2018-05-16 | 2018-05-16 | A kind of bactericidal composition |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114868770A (en) * | 2022-06-25 | 2022-08-09 | 石河子大学 | A kind of agricultural composition containing bacterial agent and γ-polyglutamic acid |
| CN115500350A (en) * | 2022-10-25 | 2022-12-23 | 上海市农业科学院 | A kind of growth inhibitor of Chrysanthemum oryzae |
-
2018
- 2018-05-16 CN CN201810472191.XA patent/CN110495456A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114868770A (en) * | 2022-06-25 | 2022-08-09 | 石河子大学 | A kind of agricultural composition containing bacterial agent and γ-polyglutamic acid |
| CN114868770B (en) * | 2022-06-25 | 2024-03-19 | 石河子大学 | Agricultural composition containing bacterial agent and γ-polyglutamic acid |
| CN115500350A (en) * | 2022-10-25 | 2022-12-23 | 上海市农业科学院 | A kind of growth inhibitor of Chrysanthemum oryzae |
| CN115500350B (en) * | 2022-10-25 | 2024-02-09 | 上海市农业科学院 | A kind of growth inhibitor of Sclerotinia oryzae |
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