CN104106580A - Compound bactericide for preventing and treating wheat scab - Google Patents

Abstract

The invention discloses a compound bactericide for preventing and treating wheat scab. The compound bactericide is prepared from albendazole and tebuconazole as base materials according to a mass ratio of albendazole to tebuconazole of 7: 1 to 1: 7. Preferably, a mass ratio of albendazole to tebuconazole is 1:2. The albendazole is a bactericide raw drug having a mass fraction of 97% and the tebuconazole is a bactericide raw drug having a mass fraction of 98.43%. The two reaction base materials in the compound bactericide do not undergo a chemical reaction and produce obvious synergism or combined effects and thus the compound bactericide is safe in use and has good poisoning activity to different types of wheat scab fungi in the field, wherein the poisoning activity is obviously higher than that of an albendazole or tebuconazole single agent. The compound bactericide is suitable for preventing and treating wheat scab in the vast rural areas.

Description

A compound fungicide for preventing and treating wheat scab

technical field

The present invention relates to fungicides, specifically, belongs to fungicides containing organic compounds, and furthermore, belongs to fungicides for preventing and treating wheat head blight. the

technical background

Wheat scab scientific name Fusarium graminearum (FusaHum graminearum Sehw), alias wheat ear dead, rotten wheat head, red wheat head, is one of the main diseases of wheat. Wheat head blight occurs widely in the world, mainly in humid and semi-humid regions, especially in temperate regions with humid and rainy climates. It can be damaged from seedlings to heading, mainly causing seedling rot, stem base rot, stalk rot and ear rot, among which the most serious damage is ear rot. Wheat head blight is caused by a variety of Fusarium fungi, collectively known as wheat head blight [Gibbrelia zeae (Schw.) Petch]. the

Albendazole (Albendazole) is an efficient, safe and broad-spectrum imidazole fungicide, which has significant control effects on various plant diseases and has a certain effect on increasing production. Not ideal; Tebuconazole is a highly efficient, broad-spectrum, systemic triazole fungicidal pesticide, but its control effect on wheat head blight is not ideal, and it also has a certain inhibitory effect on wheat growth. These two pesticides have unsatisfactory control effects on wheat scab respectively. the

In recent years, the problem of preventing and treating wheat scab has attracted people's attention, and some related fungicides have been developed, such as Chinese patent application No. 00112638.5 "Plant active oligosaccharide pesticides for preventing and controlling wheat scab and other crop diseases", 200810022910.4 No. 201010202322.6 "A Fungicide for Controlling Wheat Scab" and No. 201110332967.6 "A Chitooligosaccharide Composition for Resistance to Wheat Scab and Its uses and methods", etc. How effective these fungicides are is unclear. At present, there is no related application for using albendazole and tebuconazole compounded as a fungicide against wheat head blight. the

Contents of the invention

The purpose of the present invention is to provide a compound fungicide for preventing and treating wheat scab, which has strong poisonous ability to wheat scab, can be applied to the vast rural areas to prevent and treat wheat scab, and make wheat, which occupies an important position in agricultural products, Increase production and income, and serve the development of the national economy. the

The inventor has repeatedly screened the pesticides with the activity of killing wheat scab, and repeatedly tested the mass ratio of the raw materials of the mixed medicament, compared the test results, and found a compound fungicide that is effective against wheat scab. Possess strong poisoning ability. the

The compound fungicide for the prevention and treatment of wheat head blight provided by the inventor is based on albendazole and tebuconazole, and is formulated according to the mass ratio of albendazole and tebuconazole in a ratio of 7:1 to 1:7. fungicide. the

After further optimization, the best mass ratio of albendazole and tebuconazole is 1:2. the

The above-mentioned albendazole is the original fungicide drug with a mass fraction of 98.9%, and the above-mentioned tebuconazole is the original fungicide drug with a mass fraction of 97.5%. the

The screening test of the compound fungicide of the above-mentioned prevention and treatment of wheat scab is as follows:

1. Purpose of the test: To determine the optimum ratio of albendazole-tebuconazole compound by using the method of mycelial growth rate with the fungus Fusarium scab as the target. the

2 Test conditions

2.1 Test target

Wheat scab [Gibbrella zeae (Schw.) Petch] was collected, isolated and identified from Zhejiang; it was stored in a refrigerator at 4°C. the

2.2 Culture conditions

After being transferred once on the PDA medium plate before identification, it was pre-cultured at 25°C for 4 days, and mycelium pieces with a diameter of 5 mm were taken from the edge of the colony for determination. the

3 Experimental design

3.1 Test drug

(1) Altimazole superfine powder original drug, with an effective content of 98.9%, provided by Guizhou Daoyuan Biotechnology Co., Ltd. the

(2) Tebuconazole technical, effective content 97.5%, provided by Changzhou Fengdeng Agrochemical Co., Ltd.;

3.2 Test treatment

3.2.1 Dose setting

The test agent albendazole was made into 10000 μg/ml mother solution with N,N-dimethylformamide; tebuconazole was made into 10000 μg/ml mother solution with methanol. Albendazole and tebuconazole were compounded in eight ratios of 7:1, 5:1, 3:1, 1:1, 1:2, 1:3, 1:5, and 1:7, respectively. Drugs were added to the AEA medium according to the series concentrations designed after the preliminary test to make drug-containing plates. The measured concentration of albendazole was 100, 50, 25, 12.5, 6.25 μg/ml; the measured concentration of tebuconazole was 2.5, 1.25, 0.625, 0.3125, 0.0156 μg/ml. See the table for the measured concentrations of the albendazole-tebuconazole compound. Set no drug plate control. the

3.2.2 Test repetition

Each concentration treatment was repeated for 4 dishes. the

4 Test method

The mycelial growth rate method was used. Pick the pre-prepared mycelium blocks and inoculate them on PDA medium plates with different drug concentrations, culture at 25°C for 4 days, check the colony diameter, calculate the percentage of mycelial growth inhibited by each drug treatment, the probability value and series of the inhibition rate The linear regression analysis between the logarithmic values of the drug concentration is used to obtain the EC ₅₀ of each drug, and the synergistic effect of the mixture is calculated according to Wadley's method, that is, if SR<0.5, the two drugs have an antagonistic effect when mixed, and 0.5≤ If SR≤1.5, the mixture of the two agents has an additive effect; if SR>1.5, the mixture of the two agents has a synergistic effect.

${\mathrm{<span\; class="notranslate">\; x</span>}}_{\mathrm{<span\; class="notranslate">\; 1</span>}}<span\; class="notranslate">\; =</span>\frac{{\mathrm{<span\; class="notranslate">\; P</span>}}_{\mathrm{<span\; class="notranslate">\; A</span>}}<span\; class="notranslate">\; +</span>{\mathrm{<span\; class="notranslate">\; P</span>}}_{\mathrm{<span\; class="notranslate">\; B</span>}}}{{\mathrm{<span\; class="notranslate">\; P</span>}}_{\mathrm{<span\; class="notranslate">\; A</span>}}<span\; class="notranslate">\; /</span>\mathrm{<span\; class="notranslate">\; A</span>}<span\; class="notranslate">\; +</span>{\mathrm{<span\; class="notranslate">\; P</span>}}_{\mathrm{<span\; class="notranslate">\; B</span>}}<span\; class="notranslate">\; /</span>\mathrm{<span\; class="notranslate">\; B</span>}}$

SR=X ₁ /X ₂

5 Toxicity determination

Albimazole and tebuconazole were independently tested according to the above 8 ratios, and the results are as follows

Evaluation of combined effects of 8 compound ratios of albendazole and tebuconazole on wheat head blight

6 Results Analysis

The EC ₅₀ value of albendazole in inhibiting the mycelial growth of S. tritici was 13.5142 μg/ml; the EC ₅₀ value of tebuconazole inhibiting the growth of S. tritici mycelium was 0.8579 μg/ml. The EC ₅₀ values and SR values of albendazole and tebuconazole compounded at the ratios of 7:1, 5:1, 3:1, 1:1, 1:2, 1:3, 1:5, and 1:7 ₅₀ values are shown in the table above, all of which show good synergistic effect; the best mass ratio is 1:2.

The compound fungicide of the present invention has no chemical reaction between the two base materials, has obvious synergistic or additive effects, is safe to use, and has very good poisonous activity against different types of wheat head blight in the field at present, and is obviously high Altimazole or tebuconazole single agent. It is suitable for controlling wheat scab in vast rural areas. the

Detailed ways

Embodiment: Take 2.5 kg of albendazole with a mass fraction of 98.9% produced by Guizhou Daoyuan Biotechnology Co., Ltd. and 5.0 kg of tebuconazole with a mass fraction of 97.5% produced by Changzhou Fengdeng Agrochemical Co., Ltd. as base materials, and compound them into 7.5kg compound fungicide for preventing and controlling wheat head blight. the

The compound fungicide was used in a certain place in Guizhou, and achieved remarkable effects in treating wheat scab. the

Claims (3)

1. A compound fungicide for preventing and treating wheat head blight, characterized in that the compound fungicide is based on albendazole and tebuconazole, according to the mass ratio of albendazole and tebuconazole 7: 1～ 1:7 prepared. 2. According to the compound fungicide according to claim 1, it is characterized in that the compound fungicide is based on albendazole and tebuconazole, prepared according to the mass ratio of albendazole and tebuconazole 1:2 into. 3. according to the compound fungicide described in claim 2, it is characterized in that described albendazole is the former medicine of fungicide of mass fraction 98.9%, and described tebuconazole is the former medicine of fungicide of mass fraction 97.5%.