CN103300016B - A kind of bactericidal composition containing Acibenzolar and dithianon - Google Patents

Abstract

The invention relates to a bactericidal composition containing activated ester and dicyanoanthraquinone, wherein the active ingredients are activated ester and dicyanoanthraquinone, and the weight ratio of activated ester to dicyanoanthraquinone is 1:80 to 60:1. The effective active ingredient can be made into wettable powder, water dispersible granule, suspension concentrate, suspoemulsion, water emulsion, microemulsion, microcapsule suspension and microcapsule suspension-suspension concentrate by adding auxiliary agents. Can be used to control rice, wheat, peach, citrus, banana, watermelon, pear, grape, flower, pepper, cucumber, rust on apple, downy mildew, mildew, scab, blight, anthracnose, ring spot , brown spot, brown rot, leaf shrinkage, scab, leaf spot, leaf spot. It has the characteristics of wide bactericidal spectrum, small dosage, resistance to rain erosion and obvious synergistic effect.

Description

A kind of bactericidal composition containing activated ester and dicyanoanthraquinone

technical field

The invention belongs to the technical field of pesticides, and relates to the application of a fungicidal composition containing activated ester and dicyanoanthraquinone on crop diseases.

technical background

Acibenzolar chemical name: methyl 1,2,3-benzothiadiazole-7-thiocarboxylate, molecular formula: C ₂₁ H ₁₆ ClF ₃ N ₄ O ₅ . Activated ester is a kind of plant disease resistance activator. It has no bactericidal activity and therapeutic effect, but it activates the plant's resistance mechanism after being absorbed by the plant, and activates the plant's own defense and resistance response, thus making the plant resistant to various Fungi, bacteria, nematodes and diseases develop broad-spectrum self-protection and long-lasting resistance.

Dithianon chemical name: 2,3-dicyano-1,4-dithioanthraquinone, molecular formula: C ₁₄ H ₄ N ₂ O ₂ S ₂ . Dicyanoanthraquinone decomposes under alkaline conditions (PH>7), cannot be mixed with mineral oil sprays, and is mainly used as a protective fungicide for various leaf diseases. It has multiple action mechanisms. It inhibits a series of fungal enzymes by reacting with sulfur-containing groups and interfering with cellular respiration, and finally leads to the death of the disease. It has good protective activity and certain therapeutic activity.

In the actual process of agricultural production, the most likely problem in disease prevention and control is the emergence of bacterial resistance. Combination of ingredients from different varieties is a very common method to prevent and control resistant bacteria. Different ingredients are compounded, and according to the actual application effect, it is judged whether a certain compound is synergistic, additive or antagonistic. In most cases, the compounding effects of pesticides are additive effects, and there are very few compoundings that really have synergistic effects, especially those with very obvious synergistic effects and high synergistic ratios. After research by the inventor, it is found that the compounding of activated ester and dicyanoanthraquinone has a good synergistic effect within a certain range, and the relevant compounding of activated ester and dicyanoanthraquinone has not been seen at home and abroad. Related reports.

Contents of the invention

The object of the present invention is to propose a bactericidal composition containing activated ester and dicyanoanthraquinone with synergistic effect, low use cost and good control effect.

A bactericidal composition containing activated ester and dicyanoanthraquinone, characterized in that the weight ratio of the effective active ingredient activated ester to dicyanoanthraquinone is 1:80-60:1.

The bactericidal composition containing activated ester and dicyanoanthraquinone is characterized in that the weight ratio of activated ester to dicyanoanthraquinone is 1:40-30:1.

The bactericidal composition containing activated ester and dicyanoanthraquinone is characterized in that the weight ratio of activated ester to dicyanoanthraquinone is 1:25-5:1.

The bactericidal composition containing activated ester and dicyanoanthraquinone is made into wettable powder, water dispersible granule, suspension, suspoemulsion, water emulsion, microemulsion, microcapsule suspension and microcapsule suspension-suspension.

The bactericidal composition containing activated ester and dicyanoanthraquinone is used for preventing and controlling diseases of rice, wheat, peach, citrus, banana, watermelon, pear, grape, flower, pepper, cucumber and apple.

The diseases include rust, downy mildew, mildew, scab, blight, anthracnose, ring spot, brown spot, brown rot, leaf shrinkage, scab, leaf spot, leaf spot .

The content of the active components in the composition of the present invention depends on the application amount when used alone, also depends on the mixing ratio of one compound and another compound and the degree of synergistic effect, and is also related to the target fungus. Usually the weight percentage of active components in the composition is 2%-90% of the total weight, preferably 5%-80%. Depending on the type of preparation, the content range of the active ingredient is different. Usually, liquid preparations contain 1% to 70% by weight of active substances, preferably 5% to 50%; solid preparations contain 5% to 80% by weight of active substances, preferably 10% to 80%. %.

The fungicidal composition of the present invention contains at least one surfactant to facilitate the dispersion of active components in water during application. The surfactant content is 5%-30% of the total weight of the preparation, and the balance is liquid diluent.

The surfactant selected for the bactericidal composition of the present invention is well known to those skilled in the art: it can be selected from one or more of dispersants, wetting agents, thickeners or defoamers. According to different dosage forms, the preparation may also contain stabilizers, antifreezing agents and the like known to those skilled in the art.

The antiseptic composition of the present invention can be diluted or directly used by users before use. Its preparation can be prepared by processing methods known to those skilled in the art, that is, after the active ingredient is mixed with a liquid solvent or a solid carrier, and then added to a surfactant such as a dispersant, a stabilizer, a wetting agent, a binder, an antifoaming agent, etc. one or more of.

The fungicidal composition of the present invention can be processed into any pesticide-acceptable dosage form as required. Among them, the preferred formulations are wettable powder, water dispersible granule, suspension, suspoemulsion, water emulsion, microemulsion, microcapsule suspension, and microcapsule suspension-suspension concentrate.

When the composition is made into wettable powder, it contains the following components: 1-60% of activated ester, 1-80% of dicyanoanthraquinone, 2-10% of dispersant, 2-8% of wetting agent and the rest of filler.

The original medicine, dispersant, wetting agent and filler in the above formula are mixed, mixed uniformly in a mixing tank, pulverized by a jet mill, and then mixed uniformly to produce a wettable powder product of the composition of the present invention.

When the composition is made into water-dispersible granules, the following components are included: 1-60% of activated ester, 1-80% of dicyanoanthraquinone, 1-10% of dispersant, 1-8% of wetting agent, 1-80% of disintegrant 10%, binder 1-8%, filler balance.

The original drug, dispersant, wetting agent, disintegrating agent, filler, etc. in the above formula are crushed together by airflow to obtain the required particle size, and then other additives such as binder are added to obtain the granulation material. The water-dispersible granule product of the composition of the present invention is obtained by quantitatively sending the material into a fluidized bed granulation dryer for granulation and drying.

When the composition is made into a suspending agent, it includes the following components: 1-40% of activated ester, 1-50% of dicyanoanthraquinone, 1-10% of dispersant, 1-10% of wetting agent, and 0.1-1% of defoamer , Thickener 0.01-2%, antifreeze agent 0.1-8%, deionized water added to 100%.

Mix the dispersant, wetting agent, defoamer, thickener, and antifreeze in the above formula evenly through high-speed shearing, add the active ester original drug and dicyanoanthraquinone original drug, and ball mill in a ball mill for 2 to 3 hours. The particle size of the particles is all below 5 μm to prepare the suspension product of the composition of the present invention.

When the composition is made into a suspoemulsion, it includes the following components: 1% to 40% of activated ester, 1% to 50% of dicyanoanthraquinone, 1% to 10% of emulsifier, 1% to 10% of dispersant, and defoamer 0.1% to 2%, thickener 0.01% to 2%, antifreeze agent 0.1% to 8%, stabilizer 0.05% to 3%, and deionized water to 100%.

Mix the dispersant, defoamer, thickener, antifreeze, and stabilizer in the above formula evenly through high-speed shearing, add the active ester raw material, and mill in a ball mill for 2 to 3 hours, so that the particle size of all particles is 5 μm Next, the activated ester suspending agent is prepared, and then the original drug of dicyanoanthraquinone, emulsifier and various auxiliary agents are directly emulsified into the suspending agent with a high-speed stirrer to obtain the suspoemulsion product of the composition of the present invention.

When the composition is made into water emulsion, the following components are included: 1%-40% of activated ester, 1%-50% of dicyanoanthraquinone, 1%-20% of solvent, 1%-12% of emulsifier, and 1% of dispersant ~10%, antifreeze 0.1%~8%, defoamer 0.1%~2%, thickener 0.01%~2%, deionized water to 100%.

Add the original drug, solvent, and emulsifier in the above formula together to dissolve into a uniform oil phase. Mix deionized water, dispersant, antifreeze, defoamer, and thickener together to form a uniform water phase, and slowly drop the water phase into the oil phase under high-speed stirring to prepare the dispersed composition of the present invention. Good emulsion in water product.

When the composition is made into a microemulsion, the following components are included: 1% to 40% of activated ester, 1% to 50% of dicyanoanthraquinone, 1% to 15% of emulsifier, 2% to 10% of solvent, 2% of antifreeze % to 10%, stabilizer 0.5% to 3%, and deionized water to 100%.

Add the original drug, solvent, and emulsifier in the above formula into the homogenizer to obtain a uniform oil phase, add deionized water, antifreeze agent, and stabilizer into the homogenizer equipped with the above solution, and then vigorously mix and homogenize Thin, mix uniformly to make the transparent or translucent microemulsion product of the composition of the present invention.

When the composition is made into a microcapsule suspension, the following components are included: 1% to 40% of activated ester, 1% to 50% of dicyanoanthraquinone, 1% to 10% of polymer capsule wall material, and 2% to 10% of dispersant %, 1% to 10% of organic solvent, 1% to 7% of emulsifier, 1% to 5% of pH regulator, and deionized water to 100%.

Mix the original drug, polymer capsule wall material and organic solvent in the above formula to dissolve into a uniform oil phase, and add the oil phase to the aqueous phase solution containing emulsifier, pH regulator and dispersant under shearing conditions, The two materials react at the oil-water interface to form a macromolecule capsule wall to prepare a well-dispersed microcapsule suspension product of the composition of the present invention.

When the composition is made into a microcapsule suspension-suspension agent, it includes the following components: 1% to 40% of activated ester, 1% to 50% of dicyanoanthraquinone, 1% to 12% of polymer capsule wall material, and 2% of surfactant %～15%, organic solvent 1%～15%, emulsifier 1%～8%, defoamer 0.2%～5%, thickener 0.01%～2%, pH regulator 0.1%～5%, deionization Water was added to 100%.

Mix the active ester raw drug, polymer capsule wall material, and organic solvent in the above formula to dissolve into a uniform oil phase, and add the oil phase to the aqueous phase solution containing an emulsifier and a pH regulator under shearing conditions. This material reacts at the oil-water interface, forms a polymer capsule wall around the droplet containing the chemical substance, and makes a well-dispersed microcapsule suspension. Mix the surfactant, defoamer, thickener, and antifreeze evenly through high-speed shearing, add the original drug of dicyanoanthraquinone, and ball mill in a ball mill for 2 to 3 hours, so that the particle size of all particles is below 5 μm. Obtain a suspending agent, and then add the suspending agent to the aqueous phase solution of the microcapsule suspending agent to prepare a well-dispersed microcapsule suspension-suspension agent product of the composition of the present invention.

Main technical index of wettable powder of the present invention:

Water-dispersible granule main technical index of the present invention:

Suspending agent main technical index of the present invention:

Suspoemulsion main technical index of the present invention:

Water-emulsion main technical index of the present invention:

Microemulsion main technical index of the present invention:

Main technical index of microcapsule suspension of the present invention:

Microcapsule suspension-suspension agent main technical index of the present invention:

Beneficial effects of the present invention: (1) the composition of the present invention has a good synergistic effect within a certain range, the preventive effect is higher than that of a single dose, and the dosage is small; (2) the organic solvent of the present invention is used in a small amount, and it is difficult to produce drug (3) The composition of the present invention expands the bactericidal spectrum, and is effective against crop rust, downy mildew, mildew, scab, blight, anthracnose, ring spot, brown spot, brown rot disease, leaf shrinkage, scab, spotted defoliation, and leaf spot all have special effects.

Detailed ways

The present invention will be further described below in conjunction with the examples. The percentages in the examples are weight ratios, but the present invention is not limited thereto.

The embodiment of the present invention adopts the method combining indoor virulence determination and field test. Application Example 1

Example 161% activated ester·dicyananthraquinone wettable powder

Activated ester 60%, dicyanoanthraquinone 1%, arylphenol polyoxyethylene succinate sulfonate 7%, sodium lauryl sulfate 6%, diatomaceous earth to 100%, the mixture is jet milled to obtain 61% activated ester · dicyanoanthraquinone wettable powder.

Example 241% activated ester·dicyananthraquinone wettable powder

40% of activated ester, 1% of dicyanoanthraquinone, 6% of alkylphenol polyoxyethylene pyrimidine, 5% of sodium lauryl sulfate, bentonite to 100%, and the mixture is jet milled to obtain 41% of activated ester·dicyananthracene Quinone wettable powder.

Example 381% activated ester·dicyananthraquinone wettable powder

1% activated ester, 80% dicyanoanthraquinone, 5% polyoxyethylene pyrimidine ester, 3% pull-off powder BX, diatomaceous earth to 100%, the mixture is jet milled to obtain 81% activated ester·dicyanoanthraquinone wettable powder.

Example 430% activated ester·dicyananthraquinone water dispersible granule

Activated ester 10%, dicyanoanthraquinone 20%, octylphenol polyoxyethylene ether sulfate 7%, sodium lauryl sulfate 5%, urea 4%, white carbon black to 100%, mixed to obtain 30% activated Ester · dicyanoanthraquinone water dispersible granules.

Example 561% activated ester · dicyanoanthraquinone water dispersible granule

Add 1% of activated ester, 60% of dicyanoanthraquinone, 8% of lignosulfonate, 7% of teaku, 2.5% of bentonite, diatomaceous earth to 100%, and mix to obtain 61% activated ester·dicyanoanthraquinone water Dispersible granules.

Example 625% activated ester·dicyananthraquinone water dispersible granule

Activated ester 5%, dicyanoanthraquinone 20%, alkylphenol polyoxyethylene pyrimaldehyde condensate sulfate 7%, pull-off powder BX 4%, sodium bicarbonate 3%, light calcium carbonate to 100%, mixed A 25% activated ester·dicyananthraquinone water-dispersible granule was obtained.

Example 741% activated ester · dicyanoanthraquinone water dispersible granule

1% activated ester, 40% dicyanoanthraquinone, 8% lignosulfonate, 3% sodium lauryl sulfate, 4% bentonite, light calcium carbonate to 100%, mixed to obtain 41% activated ester. Dicyanoanthraquinone water dispersible granules.

Example 830% Activated Ester·Dicyananthraquinone Suspension Concentrate

Activated ester 5%, dicyanoanthraquinone 25%, naphthalenesulfonic acid formaldehyde condensate 8%, saponin powder 5%, silicone oil 0.2%, hydroxymethyl cellulose 0.09%, ethylene glycol 2.8%, deionized water to 100%, mixed to prepare 30% activated ester·dicyananthraquinone suspension concentrate.

Example 9 10% Activated Ester·Dicyananthraquinone Suspension Concentrate

Activated ester 5%, dicyanoanthraquinone 5%, fatty amine polyoxyethylene pyrimidine 5%, sodium lauryl sulfate 6%, silicone compound 0.3%, xanthan gum 0.12%, diethylene glycol 2%, Add ionized water to 100%, and mix to prepare 10% activated ester·dicyananthraquinone suspension concentrate.

Example 10 33% Activated Ester·Dicyananthraquinone Suspension Concentrate

3% activated ester, 30% dicyanoanthraquinone, 7% polyoxyethylene pyrimidine ester, 3% sodium dodecylbenzenesulfonate, 0.3% silicone oil, 0.18% aluminum magnesium silicate, 0.15% polyethylene glycol, Add ionized water to 100%, and mix to prepare 33% activated ester·dicyananthraquinone suspension concentrate.

Example 1115% activated ester·dicyananthraquinone suspoemulsion

Activated ester 5%, dicyanoanthraquinone 10%, lignosulfonate 6%, wetting and penetrating agent F5%, methyl cellulose 0.18%, Tween series 4%, propylene glycol 2%, silicone 0.3%, Add water to 100%, and mix to obtain 15% activated ester·dicyananthraquinone suspoemulsion.

Example 1240% activated ester·dicyananthraquinone suspoemulsion

Activated ester 8%, dicyanoanthraquinone 32%, fatty alcohol polyoxyethylene ether 6%, saponin powder 6%, castor oil polyoxyethylene ether 5.5%, silicone oil 0.2%, polyvinylpyrrolidone 0.16%, polyethylene glycol Add 0.17% alcohol and deionized water to 100%, and mix to prepare 40% activated ester·dicyananthraquinone suspoemulsion.

Example 13 35% activated ester·dicyananthraquinone suspoemulsion

Activated ester 5%, dicyanoanthraquinone 30%, bis(alkyl) naphthalene sulfonate formaldehyde condensate 8%, pull-off powder BX 2%, xanthan gum 0.07%, phenethylphenol polyoxyethyl polypropylene Add 5% ether, 2.6% triethylene glycol, 0.2% silicone oil, and water to 100%, and mix to prepare a 35% activated ester·dicyananthraquinone suspoemulsion.

Example 14 30% activated ester · dicyanoanthraquinone suspoemulsion

Activated ester 20%, dicyanoanthraquinone 10%, lignosulfonate 4%, sapinberry powder 5%, sodium tripolyphosphate 0.07%, polyethylene glycol 0.12%, silicone compound 0.3%, fatty alcohol Add 7% polyoxyethylene ether and water to 100%, and mix to prepare 30% activated ester·dicyananthraquinone suspoemulsion.

Example 15 26% activated ester · dicyanoanthraquinone alcohol water emulsion

Activated ester 25%, dicyanoanthraquinone 1%, cyclohexanone 5%, vegetable oil 4%, sorbitan monostearate 6%, diethylene glycol 2.2%, silicone oil 0.3%, deionized water to 100% , mixed to obtain 26% activated ester · dicyanoanthraquinone alcohol aqueous emulsion.

Example 16 22% activated ester · dicyanoanthraquinone aqueous emulsion

20% activated ester, 2% dicyanoanthraquinone, 5% cyclohexanone, 3% butanol, 6% calcium alkylbenzene sulfonate, 2.4% glycerol, 0.6% silicone, deionized water to 100 %, 22% activated ester · dicyanoanthraquinone emulsion in water was prepared by mixing.

Example 17 32% activated ester · dicyanoanthraquinone aqueous emulsion

Activated ester 30%, dicyanoanthraquinone 2%, styrene polyoxyethylene ether ammonium sulfate 6%, alkylaryl polyoxyethylene ether 5%, castor oil polyoxyethylene ether 6%, glycerol 5%, 0.15% of gum arabic, 0.3% of C _10-20 saturated fatty acids, and 100% of deionized water were added to prepare a 32% activated ester·dicyanoanthraquinone emulsion in water.

Example 18 30% activated ester · dicyanoanthraquinone aqueous emulsion

Activated ester 25%, dicyanoanthraquinone 5%, trimethylcyclohexenone 7%, acetone 3%, ethylene oxide-propylene oxide block copolymer 5%, triethylene glycol 2%, C _{8～ 10} Add 0.2% of fatty alcohols and deionized water to 100%, and mix to prepare 30% activated ester·dicyanoanthraquinone water emulsion.

Example 19 16% activated ester · dicyanoanthraquinone microemulsion

Activated ester 1%, dicyanoanthraquinone 15%, trimethylcyclohexenone 5%, diethylene glycol 6.3%, xanthan gum 0.06%, fatty alcohol polyoxyethylene ether 5%, arylphenol polyoxyethylene butyl Diacid ester sulfonate 4%, silicones 0.5%, deionized water make up 100%, and 16% activated ester·dicyananthraquinone microemulsion was prepared.

Example 2020% activated ester · dicyanoanthraquinone microemulsion

Activated ester 15%, dicyanoanthraquinone 5%, ethyl acetate 4%, acetonitrile 3%, castor oil polyoxyethylene ether 9%, ethylene glycol 2%, C _8-10 fatty alcohol 3%, deionized water Add to 100%, and mix to prepare 20% activated ester·dicyananthraquinone microemulsion.

Example 21 25% activated ester · dicyanoanthraquinone microemulsion

Activated ester 20%, dicyanoanthraquinone 5%, propanol 6%, N,N-dimethylformamide 4%, sorbitan monostearate 7%, polyvinyl alcohol 0.15%, silicone oil 0.4%, Add deionized water to 100%, and mix to prepare 25% activated ester·dicyananthraquinone microemulsion.

Example 2231% activated ester · dicyanoanthraquinone microemulsion

Activated ester 30%, dicyanoanthraquinone 1%, propanol 5%, N-pyrrolidone 4%, benzyl dimethylphenol polyoxyethyl ether 6%, polyethylene glycol 0.03%, silicones 0.2%, Add deionized water to 100%, and mix to prepare 31% activated ester·dicyananthraquinone microemulsion.

Example 2340% activated ester · dicyananthraquinone microcapsule suspension

Activated ester 10%, dicyanoanthraquinone 30%, bis(alkyl) naphthalene sulfonate formaldehyde condensate 4%, sodium lauryl sulfate 6%, silicones 2%, hydroxymethyl cellulose 0.06%, Add deionized water to 100%, and mix to prepare 40% activated ester·dicyananthraquinone microcapsule suspension.

Example 24 31% activated ester·dicyananthraquinone microcapsule suspension

Activated ester 1%, dicyanoanthraquinone 30%, naphthalenesulfonic acid formaldehyde condensate 5%, wetting and penetrating agent F4%, polyisocyanate 6%, sodium tripolyphosphate 0.05%, deionized water to 100%, mixed A 31% activated ester·dicyananthraquinone microcapsule suspension was obtained.

Example 25 26% Activated Ester·Dicyananthraquinone Microcapsule Suspension Concentrate

Activated ester 1%, dicyanoanthraquinone 25%, arylphenol polyoxyethylene succinate sulfonate 3%, pull-off powder BX 5%, C _10-20 saturated fatty acids 1.5%, hydroxyethyl cellulose 0.05 % and deionized water to 100%, mixed to prepare 26% activated ester·dicyananthraquinone microcapsule suspension.

Example 26 20% Activated Ester·Dicyananthraquinone Microcapsule Suspension-Suspension Concentrate

Activated ester 2%, dicyanoanthraquinone 18%, maltose 0.8%, gum arabic 0.13%, β-cyclodextrin 0.9%, gelatin 0.1%, propylene glycol polyoxyethylene polyoxypropylene block polyether 2.4%, methylene Binaphthalene sulfonate 5.5%, propanol 2%, vegetable oil 8%, solvent oil 5%, polyethylene glycol 0.07%, triethanolamine salt 4%, By series 3%, silicone defoamer 0.1%, pH adjustment Sodium hydroxide and hydrochloric acid totaled 2.1%, deionized water was added to 100%, and mixed to prepare 20% activated ester·dicyananthraquinone microcapsule suspension-suspension concentrate.

Example 2740% Activated Ester·Dicyananthraquinone Microcapsule Suspension-Suspension Concentrate

Activated ester 4%, dicyanoanthraquinone 36%, maltose 0.8%, gum arabic 0.06%, β-cyclodextrin 0.9%, gelatin 0.1%, propylene glycol polyoxyethylene polyoxypropylene block polyether 2.4%, methylene Binaphthalene sulfonate 5.5%, propanol 2%, vegetable oil 8%, solvent oil 5%, polyethylene glycol 0.08%, triethanolamine salt 4%, By series 3%, silicone defoamer 0.1%, pH adjustment Sodium hydroxide and hydrochloric acid totaled 2.1%, deionized water was added to 100%, and mixed to prepare 40% activated ester·dicyananthraquinone microcapsule suspension-suspension concentrate.

The embodiment of the present invention adopts the method combining indoor virulence determination and field test. First, through the indoor toxicity test, the synergistic ratio (SR) of the two agents after compounding in a certain proportion is clarified. SR<0.5 is an antagonistic effect, 0.5≤SR≤1.5 is an additive effect, and SR>1.5 is a synergistic effect. On this basis, field experiments were carried out.

Test method: After pre-testing to determine the effective inhibitory concentration range of each drug, 5 doses of drugs were set according to the content of active ingredients, and water was used as a control. Refer to the "Pesticide Indoor Bioassay Test Guidelines for Fungicides", and use the mycelium growth rate method to determine the toxicity of the pesticides to crop pathogens. After 72 hours, the colony diameter was measured by the cross method, and the net growth and mycelial growth inhibition rate of each treatment were calculated.

Net growth (mm) = measured colony diameter -5

The mycelia growth inhibition rate was converted into a probability value (y), the concentration of the medicinal solution (μg/mL) was converted into a logarithmic value (x), and the regression equation of virulence (y=a+bx) was obtained by the least square method, and by This calculates the _EC50 value for each agent. At the same time, according to the Wadley method, the synergistic ratio (SR) of the combination of two drugs with different ratios was calculated. SR<0.5 was regarded as antagonistic effect, 0.5≤SR≤1.5 was regarded as additive effect, and SR>1.5 was regarded as synergistic effect. Calculated as follows:

Wherein: a, b are respectively the proportions of the active ingredient activated ester and dicyanoanthraquinone in the combination;

A is an activated ester;

B is dicyanoanthraquinone.

Implement application example two:

Indoor toxicity determination of activated ester and dicyanoanthraquinone against cucumber downy mildew.

All test agents were provided by Shaanxi Welch Crop Protection Co., Ltd.

Experimental design: The effective inhibitory concentration range of activated ester, dicyanoanthraquinone and their mixtures with different ratios was determined through preliminary experiments.

Toxicity test results

Table 1 Analysis table of toxicity test results of activated ester and dicyanoanthraquinone compounded to cucumber downy mildew

It can be seen from Table 1 that the EC ₅₀ of activated ester and dicyanoanthraquinone against cucumber downy mildew are 5.78mg/L and 13.16mg/L, respectively. When the ratio of activated ester to dicyanoanthraquinone is 1:80-60:1, the synergistic ratio SR is greater than 1.5, indicating that the mixture of activated ester and dicyanoanthraquinone in the range of 1:80-60:1 is uniform. Shows synergistic effect, especially when the ratio of the two is 1:25-5:1, the synergistic effect is more prominent, and when the weight ratio of activated ester to dicyanoanthraquinone is 1:5, the synergistic ratio The SR is the largest and the synergistic effect is the most obvious. Tests have found that when the ratio of activated ester to dicyanoanthraquinone is 5:1, 4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5 , 1:6, 1:7, 1:8, 1:9, 1:10, 1:11, 1:12, 1:13, 1:14, 1:15, 1:16, 1:17, 1 : 18, 1: 19, 1: 20, 1: 21, 1: 22, 1: 23, 1: 24, 1: 25 against rust, downy mildew, mildew, scab, blight, anthracnose , ring spot, brown spot, brown rot, leaf shrinkage, scab, spotted leaf spot, leaf spot, the synergistic effect is obvious, and the synergistic ratio is greater than 1.5.

Application Example Three Activated Ester and Dicyanoanthraquinone and Their Compound Control Efficacy Test on Banana Leaf Spot

This experiment was arranged in Nanning, Guangxi. The test agents were developed and provided by Shaanxi Welch Crop Protection Co., Ltd., and the control agents were 24% activated ester suspension concentrate (self-prepared) and 66% dicyanoanthraquinone water-dispersible granules (commercially purchased).

The condition of banana leaf spot was investigated before the medicine, and the medicine was applied for the first time in the early stage of the disease, and once every 7 days, and the medicine was applied 3 times in total. 3 days, 7 days, and 14 days after the third application, the disease index was investigated and the control effect was calculated. The experimental results are as follows:

Table 2 The efficacy test of activated esters, dicyanoanthraquinone and their compound in the control of banana leaf spot

It can be seen from Table 2 that the combination of activated ester and dicyanoanthraquinone can effectively control banana leaf spot, and the control effect is better than that of a single agent, and the control effect period is long. It has no adverse effect on the target crops within the range of the test drug, and it also has a good control effect on banana scab.

Application Example Four Activated Ester and Dicyanoanthraquinone and Their Compound Control Efficacy Test of Cucumber Anthracnose

This experiment is arranged in the outskirts of Qingdao City, Shandong Province. The test agent is developed and provided by Shaanxi Welch Crop Protection Co., Ltd., and the contrast agent 24% activated ester suspension concentrate (self-preparation), 66% dicyanoanthraquinone water dispersible granule (city purchase).

Investigate the condition of cucumber anthracnose before the drug treatment, apply the drug for the first time in the early stage of the disease, and apply the drug once every 7 days, and apply the drug twice in total. 3 days, 7 days, and 14 days after the second application of the drug, the disease index was investigated and the control effect was calculated. The experimental results are as follows:

Table 3 Efficacy tests of activated esters, dicyanoanthraquinone and their compound in the control of cucumber anthracnose

It can be seen from Table 3 that the combination of activated ester and dicyanoanthraquinone can effectively control cucumber anthracnose, and the control effect is better than that of a single agent, and the control effect period is long. It has no adverse effect on the target crops within the range of the test drug, and has a good control effect on cucumber downy mildew.

Application Example 5 Activated Ester and Dicyanoanthraquinone and Their Compound Control Efficacy Test of Apple Spotted Leaf Blight

This experiment is arranged in Xianyang City, Shaanxi Province. The test agent is developed and provided by Shaanxi Welch Crop Protection Co., Ltd., and the contrast agent 24% activated ester suspension concentrate (self-prepared), 66% dicyanoanthraquinone water dispersible granule (commercially available) ).

The condition of apple spotted leaf defoliation was investigated before the medicine, and the medicine was applied for the first time at the early stage of the disease, and once every 7 days, for a total of 3 times. 3 days, 7 days, and 14 days after the third application, the disease index was investigated and the control effect was calculated. The experimental results are as follows:

Table 4 Efficacy tests of activated esters, dicyanoanthraquinone and their compound in the control of apple spot defoliation

It can be seen from Table 4 that the combination of activated ester and dicyanoanthraquinone can effectively prevent and control apple spot defoliation, and the control effect is better than that of a single agent, and the control effect period is long. There is no adverse effect on the target crops within the scope of the test drug, and the applicant also found that the combination of the activated ester and dicyanoanthraquinone also has a good control effect on apple ringworm, and the control effect is as high as 96%.

Application Example Six Activated Ester and Dicyanoanthraquinone and Their Compound Control Efficacy Test on Grape Downy Mildew

This experiment is arranged in the suburbs of Weinan City, Shaanxi Province. The test medicament is developed and provided by Shaanxi Welch Crop Protection Co., Ltd., and the contrast agent 24% activated ester suspension concentrate (self-preparation), 66% dicyanoanthraquinone water dispersible granule (city purchase).

The disease condition of grape downy mildew was investigated before the medicine, and the medicine was applied for the first time in the early stage of the disease, once every 7 days, and the medicine was applied twice in total. 3 days, 7 days, and 14 days after the second application, the disease index was investigated and the control effect was calculated. The experimental results are as follows:

Table 5 Efficacy test of activated ester, dicyanoanthraquinone and their compound in controlling grape downy mildew

It can be seen from Table 5 that the combination of activated ester and dicyanoanthraquinone can effectively control grape downy mildew, and the control effect is better than that of a single agent, and the control effect period is long. It has no adverse effect on the target crops within the range of the test drug, and it also has a good control effect on grape anthracnose.

Application Example 7 Activated Ester and Dicyanoanthraquinone and Their Compound Control Efficacy Test of Pear Tree Scab

This experiment was arranged in Liquan County, Xianyang City, Shaanxi Province. The test agents were developed and provided by Shaanxi Welch Crop Protection Co., Ltd. The control agents were 24% activated ester suspension concentrate (self-made), 66% dicyanoanthraquinone water-dispersible granules (commercially available).

Investigate the condition of pear scab before the medicine, apply the medicine for the first time in the early stage of the disease, and apply the medicine once every 7 days, and apply the medicine for 3 times in total. 3 days, 7 days, and 14 days after the third application, the disease index was investigated and the control effect was calculated. The experimental results are as follows:

Table 6 Efficacy Test of Activated Ester, Dicyanoanthraquinone and Their Compounds in Controlling Pear Scab

It can be seen from Table 6 that the combination of activated ester and dicyanoanthraquinone can effectively control pear scab, and the control effect is better than that of a single agent, and the control effect period is long. There is no adverse effect on the target crops within the scope of the test drug.

Claims (6)

1. A bactericidal composition containing activated ester and dicyanoanthraquinone, characterized in that the weight ratio of the effective active ingredient activated ester to dicyanoanthraquinone is 1:80-40:1. 2. A bactericidal composition containing activated ester and dicyanoanthraquinone according to claim 1, wherein the weight ratio of activated ester to dicyanoanthraquinone is 1:40-30:1. 3. A bactericidal composition containing activated ester and dicyanoanthraquinone according to claim 2, wherein the weight ratio of activated ester to dicyanoanthraquinone is 1:25-5:1. 4. a kind of bactericidal composition containing activated ester and dicyanoanthraquinone according to claim 1, is characterized in that: the dosage form of bactericide composition is selected from wettable powder, water dispersible granule, suspending agent, suspoemulsion , Emulsion in water, microemulsion, microcapsule suspension, microcapsule suspension-suspension concentrate. 5. A kind of fungicidal composition containing activated ester and dicyanoanthraquinone according to any one of claims 1 to 4 is used to control rice, wheat, peach, citrus, banana, watermelon, pear, grape, flower, Application of diseases on peppers, cucumbers, and apples. 6. The application according to claim 5, characterized in that: the diseases are rust, downy mildew, mildew, scab, blight, anthracnose, ring spot, brown spot, brown rot , leaf shrinkage, scab, spotted defoliation, leaf spot.