CN115956568B - A kind of bactericidal composition containing flutriafol - Google Patents

Abstract

The present invention belongs to the technical field of pesticides, and specifically belongs to a fungicide composition containing flutriafol. A fungicide composition containing flutriafol is compounded with flutriafol and carbendazim, thiram or enoxamyl. The fungicide composition of the present invention has different action mechanisms from carbendazim, thiram or enoxamyl. When used in combination, the fungicide composition can inhibit pathogens from different action sites, thereby delaying the development of pathogen resistance and extending the service life of the agent. In addition, the fungicide composition of the present invention shows a synergistic effect on inhibiting wheat fusarium head blight pathogens, can improve the control effect of wheat fusarium head blight, thereby reducing the application dosage of pesticides and reducing the control cost.

Description

A kind of bactericidal composition containing flutriafol

Technical Field

The invention belongs to the technical field of pesticides, and in particular belongs to a fungicide composition containing flutriafol.

Background Art

Fusarium fusarium, powdery mildew, sheath blight, rust and leaf blight are important fungal diseases that threaten wheat production. Among them, wheat fusarium fusarium is mainly caused by Fusarium graminearum. The occurrence of wheat fusarium fusarium not only causes a reduction in wheat production, but also reduces wheat quality due to the production of fungal toxins, affecting the health of consumers.

In the actual production process, the prevention and control of wheat fusarium head blight mainly adopts a comprehensive prevention and control method based on disease-resistant varieties and focusing on chemical control. However, the breeding of disease-resistant varieties has a series of problems such as a long resistance improvement cycle and a lack of high-efficiency resistance planting resources, which makes chemical agents still the traditional Chinese medicine means of preventing and controlling wheat fusarium head blight. Commonly used agents for the prevention and control of wheat fusarium head blight include: carbendazim, metconazole, tebuconazole and prochloraz, but due to years of continuous use, the resistance problem has developed rapidly, the detection rate of resistant strains has increased sharply, and the prevention effect has decreased year by year. Therefore, it is necessary to develop new alternative agents or new compound agents.

Flutriafol is a triazole sterol demethylation inhibitor fungicide developed by Syngenta Group Co., Ltd. Flutriafol can effectively inhibit the biosynthesis of ergosterol and cause the rupture of fungal cell walls. It has a broad spectrum of antibacterial activity and strong systemicity, and has good protective and therapeutic effects on many fungal diseases caused by basidiomycetes and ascomycetes. CN201410788209.9 discloses a composite composition and fungicide containing mesifenazole and flutriafol, and specifically discloses that mesifenazole and flutriafol can be used to prevent and treat bacterial and fungal diseases, such as rice bacterial leaf streak, citrus canker, tomato bacterial wilt and peanut leaf spot. In addition, CN201310061385.8 also discloses a fungicide composition containing fluopyram, and specifically discloses that fluopyram and flutriafol have high activity against a variety of diseases on a variety of crops after compounding, and have obvious synergistic effects and expanded fungicidal spectrum.

So far, there are no reports on the combination of flutriafol and carbendazim, thiram or enoxamil.

The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention and should not be regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art.

Summary of the invention

The object of the present invention is to provide a fungicide composition containing flutriafol, which has a synergistic effect on inhibiting the growth of wheat fusarium scurf disease pathogens, can improve the control effect of wheat fusarium scurf disease, and solve the problems existing when using a single chemical agent.

To achieve the above object, the present invention provides the following technical solutions:

A fungicide composition containing flutriafol is prepared by compounding flutriafol with carbendazim, thiram or enoxamil.

Preferably, the mass ratio of flutriafol to carbendazim is 1-40:20-1.

Preferably, the mass ratio of flutriafol to thiram is 1-10:70-1.

Preferably, the mass ratio of flutriafol to thiram is 1:8.

Preferably, the mass ratio of flutriafol to enoxadone is 1-50:50-1.

Compared with the prior art, the present invention has the following beneficial effects:

(1) Flutriafol and carbendazim, thiram or enoxamyl in the fungicidal composition of the present invention have different action mechanisms. When used in combination, they can inhibit pathogens from different action sites, thereby delaying the development of pathogen resistance and extending the service life of the agent.

(2) The fungicidal composition of the present invention exhibits a synergistic effect on inhibiting wheat fusarium scurf pathogens, which can improve the control effect of wheat fusarium scurf, thereby reducing the application dosage of pesticides and reducing the control cost.

DETAILED DESCRIPTION

The present invention can be better understood according to the following examples. However, it is easy for those skilled in the art to understand that the contents described in the examples are only used to illustrate the present invention, and should not and will not limit the present invention described in detail in the claims.

Example : Screening of control agents

1. Test strain: Fusarium graminearum, collected from diseased wheat plants in the Xinlianxin Experimental Base in Langgongmiao Town, Xinxiang County, Henan Province, isolated in the laboratory, and preserved on PDA medium.

2. Test Drugs

95% flutriafol TC, 98% carbendazim TC (commercially available), 96% thiram TC (commercially available), 98% enoxadone TC (commercially available)

The test agent was first dissolved in dimethyl sulfoxide and then diluted with 0.1% Tween-80 aqueous solution to prepare a single-dose stock solution. Multiple groups of proportions were set, and each single dose and proportioned mixture was set with 5 mass concentration gradients according to the equal ratio method.

3. Test methods

The mycelium growth rate method was used. 9 mL of pre-melted PDA medium was added to a sterile conical flask, and 1 mL of the drug solution was quantitatively drawn from low concentration to high concentration, respectively, and added to the above conical flasks. After being fully shaken, it was poured into a culture dish with a diameter of 9 cm to make a drug-containing plate of the corresponding concentration; and a treatment without drug was set as a blank control, and 10 replicates were set for each treatment. A 5 mm diameter puncher was used to cut the bacterial cake at the edge of the test strain colony, inoculated in the center of the drug-containing plate and the blank control plate, covered with a dish cover, and placed in a 25°C constant temperature box for culture. When the diameter of the blank control colony reached more than 2/3 of the diameter of the culture dish, the colony diameter was measured using the cross method, and the mycelium growth inhibition rate of different treatments was calculated.

4. Data analysis: DPS software was used for data statistical analysis. Linear regression was performed with the logarithmic value of fungicide concentration as x and the corresponding probability value of mycelium growth inhibition rate as y to obtain the toxicity regression equation and the toxicity _EC50 value of the agent to the target pathogen. The co-toxicity coefficient (CTC) was calculated according to the Sun Yunpei method.

In the above formula: ATI - the toxicity index of the mixture measured; S - the EC ₅₀ of the standard agent, the unit is mg/L; M - the EC ₅₀ of the mixture, the unit is mg/L.

TTI＝TI _A × _PA +TI _B × _PB

In the above formula: TTI is the theoretical toxicological index of the mixture; TI _A is the toxicity index of agent A; _PA is the percentage of agent A in the mixture, in percentage (%); TI _B is the toxicity index of agent B; _PB is the percentage of agent B in the mixture, in percentage (%).

In the above formula: CTC--co-toxicity coefficient; ATI--actual toxicity index of the mixture; TTI--theoretical toxicity index of the mixture.

5. Measurement results

The synergistic effect of the drug was evaluated based on the calculated co-toxicity coefficient (CTC), CTC≤80 was antagonistic effect, 80＜CTC＜120 was additive effect, and CTC≥120 was synergistic effect. The results are shown in Table 1-3.

Table 1 Indoor bioactivity determination of flutriafol and carbendazim against Fusarium graminearum

Drug name and ratio EC50(mg/L) ATI TTI CTC Flutriafol 4.1159 100.0000 -- -- Carbendazim 0.3162 1301.6762 -- -- Flutriafol 1: Carbendazim 20 0.2294 1794.2023 1244.4535 144.1759 Flutriafol 1: Carbendazim 10 0.2681 1535.2107 1192.4329 128.7461 Flutriafol 1: Carbendazim 8 0.1931 2131.4863 1168.1566 182.4658 Flutriafol 1: Carbendazim 5 0.2300 1789.5217 1101.3968 162.4775 Flutriafol 1: Carbendazim 1 0.3826 1075.7710 700.8381 153.4978 Flutriafol 5: Carbendazim 1 0.9433 436.3299 300.2794 145.3080 Flutriafol 8: Carbendazim 1 1.4313 287.5638 233.5196 123.1433 Flutriafol 10: Carbendazim 1 1.5370 267.7879 209.2433 127.9792 Flutriafol 20: Carbendazim 1 0.8212 501.2056 157.2227 318.7871 Flutriafol 40: Carbendazim 1 2.3874 172.4009 129.3092 133.3246

As shown in Table 1, after flutriafol and carbendazim were mixed, the co-toxicity coefficients against Fusarium graminearum were all greater than 120 in the mass ratio range of 1-40:20-1, showing a synergistic effect.

Table 2 Indoor bioactivity determination of flutriafol and thiram against Fusarium graminearum

As shown in Table 2, after flutriafol and thiram were mixed, the co-toxicity coefficient against Fusarium graminearum was greater than 120 in the mass ratio range of 1-10:70-1, showing a synergistic effect. Especially when the mass ratio was 1:8, the co-toxicity coefficient reached 608.7198, and the synergistic effect was most significant.

Table 3 Indoor bioactivity determination of flutriafol and enoxamyl against Fusarium graminearum

Drug name and ratio EC50(mg/L) ATI TTI CTC Flutriafol 4.1159 100.0000 -- -- Enoxabiodinium 1.6719 246.1810 -- -- Flutriafol 1: Enoxabiodinium 50 1.0049 409.5830 243.3147 168.3347 Flutriafol 1: Enoxabiodinium 40 0.8835 465.8630 242.6156 192.0169 Flutriafol 1: Enoxabiodinium 20 0.7384 557.4079 239.2200 233.0106 Flutriafol 1: Enoxabiodinium 10 0.5685 723.9930 232.8918 310.8709 Flutriafol 1: Enoxabiodinium 8 0.9279 443.5715 229.9387 192.9086 Flutriafol 1: Enoxabiodinium 5 1.3108 313.9991 221.8175 141.5574 Flutriafol 1: Enoxabiodinium 1 0.9843 418.1550 173.0905 241.5817 Flutriafol 5: Enoxabiodinium 1 2.0950 196.4630 124.3635 157.9748 Flutriafol 8: Enoxabiodinium 1 2.4564 167.5582 116.2423 144.1456 Flutriafol 10: Enoxabiodinium 1 1.2957 317.6584 113.2892 280.3961 Flutriafol 20: Enoxabiodinium 1 1.9022 216.3758 106.9610 202.2941 Flutriafol 40: Enoxabiodinium 1 2.7531 149.5006 103.5654 144.3538 Flutriafol 50: Enoxabiodinium 1 2.2547 182.5476 102.8663 177.4610

As shown in Table 3, after flutriafol and enoxamyl are compounded, the co-toxicity coefficients against Fusarium graminearum are all greater than 120 in the mass ratio range of 1-50:50-1, showing a synergistic effect.

In summary, when flutriafol and carbendazim, thiram or enoxamyl are compounded in the fungicidal composition of the present invention, they show a synergistic effect in inhibiting the pathogenic bacteria of wheat fusarium scaber, which can improve the control effect of wheat fusarium scaber, thereby reducing the application dosage of pesticides and reducing the control cost.

The above is only a preferred embodiment of the present invention. Those skilled in the art may make appropriate improvements without departing from the principle of the present invention. These improvements are also within the protection scope of the present invention.

Claims (1)

1. A use of a fungicide composition containing flutriafol in preventing and controlling wheat fusarium scab, characterized in that the fungicide composition containing flutriafol is compounded by flutriafol and enoxamyl, and the mass ratio of flutriafol to enoxamyl is 1-50:50-1.