CN116746576A - Biological pesticide for preventing and treating rust disease of cane shoots - Google Patents

Abstract

The invention belongs to the technical field of pesticide research and development, and particularly relates to a biological pesticide for preventing and treating rust disease of cane shoots. A biological pesticide for preventing and treating rust disease of cane shoot comprises carvacrol and diniconazole or carbendazim as effective components. The carvacrol and diniconazole or carbendazim binary combination in the biological pesticide has a synergistic effect on cane shoot rust disease, can improve the prevention and treatment effect of cane shoot rust disease, is beneficial to reducing the pesticide application dosage, reduces the prevention and treatment cost and reduces the pesticide residue in cane shoots.

Description

A kind of biopesticide for preventing and controlling wild rice rust

Technical field

The invention belongs to the technical field of pesticide research and development, and specifically relates to a biological pesticide for preventing and treating wild rice rust.

Background technique

Wild rice is the second largest aquatic vegetable in my country and is also a unique vegetable in my country. Wild rice rust is caused by infection by the Basidiomycota fungus Uromyces coronatus and is a major disease in wild rice production. Wild rice rust mainly damages the leaves of wild rice. In severe cases, it may even cause the whole leaf to wither and turn yellow, and the wild rice produced will be very thin and small, which will cause great harm to the yield of wild rice. Data show that the incidence rate of wild rice leaves can reach more than 70%, and the yield loss can reach 15-30%.

Continuous planting of a single wild rice variety for many years has resulted in frequent outbreaks of wild rice rust. In the absence of excellent disease-resistant varieties, chemical control is still the main way to control the epidemic of the disease. Commonly used chemicals include 25% pyraclostrobin water-dispersed Granules, 10% difenoconazole water-dispersible granules and 12.5% difenconazole wettable powder, etc. The application of chemical agents is one of the most effective means of preventing and controlling plant diseases and insect pests. Preventing and controlling plant diseases and insect pests with chemical agents can easily lead to the development of drug resistance. At the same time, long-term continuous high-dose application of a single chemical agent can easily cause a series of problems such as agent residues and environmental pollution. . This also makes the existing chemical agents less effective in preventing and controlling wild rice rust year by year. Therefore, it is necessary to develop new agents to improve the control effect on wild rice rust and ensure the yield and quality of wild rice.

Chemical agents with different components are compounded, and based on the actual application effect, it is judged whether a certain compound is synergistic, additive or antagonistic. Among them, formulas with good synergy can significantly improve the actual control effect and reduce the dosage of pesticides, which can greatly delay the development of pesticide resistance in pests and diseases. It is one of the effective methods for developing new pesticides.

Biopesticides refer to preparations that use living organisms or their metabolism to kill or inhibit agricultural harmful organisms, including plant-derived pesticides, animal-derived pesticides and microbial-derived pesticides. Carvacrol is a pure botanical fungicide extracted and processed from goldenrod buds. It has a strong antibacterial effect and can effectively inhibit the growth of pathogenic spores and hyphae. It is safe, environmentally friendly and drug-free. It is less harmful and has good control effect on many fungal diseases such as powdery mildew, rust and downy mildew. Application number CN201510084511.0 discloses a bactericidal composition containing penflufen and carvacrol and its use. Specifically, it discloses that the compound of penflufen and carvacrol will cause harmful effects on wheat, fruit trees, flowers and vegetables. It has good control effect on powdery mildew or rust.

Through indoor biological activity tests, the inventor found that carvacrol showed a synergistic effect on the prevention and treatment of wild rice rust when compounded with silothiostrobin, diconazole or carbendazim.

At present, there are no relevant reports on the compounding of carvacrol with silothiostrobin, diconazole or carbendazim.

Contents of the invention

The purpose of the present invention is to provide a biopesticide for preventing and treating wild rice rust, which can improve the control effect of wild rice rust, reduce the dosage of pesticides, delay the development of drug resistance of pathogenic bacteria, and can be used to develop new pharmaceuticals.

In order to achieve the above objects, the present invention provides the following technical solutions:

A biopesticide for preventing and treating wild rice rust, the active ingredient of which is a binary compound of carvacrol and diconazole or carbendazim.

Preferably, the mass ratio of carvacrol and enazine alcohol is 1-9:20-1.

Preferably, the mass ratio of carvacrol and carbendazim is 1-40:15-1.

Another object of the present invention is to provide a pesticide preparation, which includes the biological pesticide for preventing and controlling wild rice rust, and the rest are auxiliary ingredients that can be added in pesticide science.

Compared with the prior art, the present invention has the following beneficial effects:

(1) The active ingredient eugenol in the biopesticide composition of the present invention has a synergistic effect on wild rice rust when compounded with diconazole or carbendazim, which can improve the control effect of wild rice rust and help reduce the dosage of pesticides. , reduce prevention and control costs and reduce pesticide residues in wild rice.

(2) The active ingredient eugenol in the biopesticide composition of the present invention has different bactericidal mechanisms from that of diconazole or carbendazim, which can effectively delay the development of drug resistance of pathogenic bacteria, extend the service life of the agent, and can be used to develop new agents.

Detailed ways

The present invention can be better understood from the following examples. However, those skilled in the art can easily understand that the content described in the embodiments is only used to illustrate the present invention, and should not and will not limit the present invention described in detail in the claims.

Example : Indoor biological activity test of carvacrol compound against wild rice rust

1. Test strain: Fresh summer spores of Coronamonas corysalis, collected from diseased water bamboo plants.

2. Test reagents

92.5% carvacrol technical material (Shanghai Yuanye Biotechnology Co., Ltd.);

98% silothiaprolin technical material (Hebei Xingbai Agricultural Science and Technology Co., Ltd.);

95% diconazole technical material (Jiangsu Qizhou Green Chemical Co., Ltd.);

98% carbendazim technical material (Jiangsu Huifeng Bio-Agriculture Co., Ltd.).

Dissolve the above-mentioned test reagents with dimethyl sulfoxide first, then dilute with 0.1% Tween-80 aqueous solution to prepare a single-dose mother solution. Set multiple groups of proportions. Each single dose and proportioned mixture are set according to the equal ratio method. 5 mass concentration gradients.

3. Test method (refer to "NY/T 1156.1-2006 Pesticide Indoor Bioassay Test Guidelines for Fungicides Part 1: Inhibition of Pathogenic Fungal Spore Germination Test Concave Slide Method").

Collect fresh summer spores from infected wild rice plants, resuspend the spores in deionized water to 1×10 ⁵ to 1×10 ⁷ spores per ml, and add 0.5% glucose solution.

Use a pipette to add 0.5 mL of the drug solution from low concentration to high concentration into small test tubes, and then pipette 0.5 mL of the prepared spore suspension to mix equal amounts of the drug solution and spore suspension evenly. Use a micropipette to absorb the above mixed solution and drop it onto a concave glass slide, then place it in a petri dish with a shallow layer of water, cover it and place it in a 25°C incubator. Each treatment was repeated three times, and the treatment with 0.1% Tween-80 aqueous solution was used as a blank control.

When the spore germination rate of the blank control reaches more than 90%, check and process the spore germination. Each treatment was repeated and randomly observed 3 fields of view, and a total of 200 spores were investigated. The number of germination and the total number of spores were recorded respectively. The length of the spore tooth tube was greater than the short radius of the spore as germination. Based on the survey data, the relative inhibition rate of spore germination in each treatment was calculated.

4. Data analysis: DPS software is used for statistical analysis of data. Using the logarithmic value of the fungicide concentration as The force EC ₅₀ value was calculated, and the cotoxicity coefficient (CTC) was calculated according to the Sun Yunpei method.

In the above formula: ATI - the measured toxicity index of the mixture; S - the EC ₅₀ of the standard agent, in mg/L; M - the EC ₅₀ of the mixture, in mg/L.

TTI＝TI _A ×P _A +TI _B ×P _B

In the above formula: TTI - the theoretical toxicological index of the mixture; TI _A - the toxicity index of agent A; P _A - the percentage content of agent A in the mixture, the unit is percentage (%); TI _B - -Toxicity index of agent B; P _B --Percentage of agent B in the mixture, the unit is percentage (%).

In the above formula: CTC - co-toxicity coefficient; ATI - measured toxicity index of mixture; TTI - theoretical toxicity index of mixture.

5. Measurement results

The synergistic effect of the agent is evaluated according to the calculated co-toxicity coefficient (CTC). CTC ≤ 80 is an antagonistic effect, 80 < CTC < 120 is an additive effect, and CTC ≥ 120 is a synergistic effect. The results are shown in Table 1-3.

Table 1 Indoor biological activity determination of the combination of carvacrol and silothiostrobin against water bamboo rust pathogens

Drug name and proportion EC ₅₀ (mg/L) ATI TTI CTC carvacrol 24.5846 100.0000 -- -- silothiaphen 9.7613 251.8578 -- -- Carvacrol 1: Silothiaphen 7 7.1211 345.2360 232.8756 148.2491 Carvacrol 1: Silothiaphen 5 6.1408 400.3485 226.5482 176.7167 Carvacrol 1: Silothiaphen 3 8.1083 303.2029 213.8934 141.7542 Carvacrol 1: Silothiaphen 1 10.0628 244.3117 175.9289 138.8696 Carvacrol 3: Silothiaphen 1 12.4932 196.7839 137.9645 142.6337 Carvacrol 5: Silothiaphen 1 9.4200 260.9830 125.3096 208.2705

It can be seen from Table 1 that after the active ingredients carvacrol and silothiaphen are compounded, the co-toxicity coefficients against the pathogenic bacteria of water bamboo rust are greater than 120 within the mass ratio of 1-5:7-1, showing a synergistic effect.

Table 2 Indoor biological activity determination of the combination of carvacrol and diconazole against water bamboo rust pathogens

It can be seen from Table 2 that after the active ingredients carvacrol and fenconazole are compounded, the co-toxicity coefficients against the pathogenic bacteria of water bamboo rust are greater than 120 within the mass ratio of 1-9:20-1, showing a synergistic effect.

Table 3 Indoor biological activity determination of carvacrol and carbendazim compound against wild rice rust pathogens

Drug name and proportion EC ₅₀ (mg/L) ATI TTI CTC carvacrol 24.5846 100.0000 -- -- carbendazim 23.1117 106.3730 -- -- Carvacrol 1: Carbendazim 15 17.0061 144.5634 105.9747 136.4132 Carvacrol 1: Carbendazim 10 16.2915 150.9045 105.7936 142.6404 Carvacrol 1: Carbendazim 5 12.5888 195.2895 105.3108 185.4411 Carvacrol 1: Carbendazim 3 14.1544 173.6887 104.7797 165.7656 Carvacrol 1: Carbendazim 1 11.0878 221.7266 103.1865 214.8795 Carvacrol 3: Carbendazim 1 18.1152 135.7126 101.5932 133.5842 Carvacrol 5: Carbendazim 1 20.0182 122.8112 101.0622 121.5205 Carvacrol 10: Carbendazim 1 16.2907 150.9119 100.5794 150.0426 Carvacrol 15: Carbendazim 1 17.1861 143.0493 100.3983 142.4818 Carvacrol 20: Carbendazim 1 13.6796 179.7172 100.3035 179.1735 Carvacrol 30: Carbendazim 1 10.7408 228.8898 100.2056 228.4203 Carvacrol 40: Carbendazim 1 12.1569 202.2275 100.1554 201.9137

It can be seen from Table 3 that after the active ingredients carvacrol and carbendazim are compounded, the co-toxicity coefficients against the pathogenic bacteria of wild rice rust are all greater than 120 within the mass ratio of 1-40:15-1, showing a synergistic effect.

In summary, the combination of eugenol with silothiacil, fenconazole or carbendazim has a synergistic effect on wild rice rust, which can improve the control effect of wild rice rust, help reduce the dosage of pesticides, and reduce the cost of prevention and control. cost and reduce pesticide residues in wild rice.

The above are only preferred embodiments of the present invention. For those skilled in the art, appropriate improvements can be made without departing from the principles of the present invention, and these improvements are also within the protection scope of the present invention.

Claims (4)

1. A biopesticide for preventing and treating wild rice rust, characterized in that its active ingredient is a binary compound of carvacrol and diconazole or carbendazim. 2. The biological pesticide for preventing and treating wild rice rust according to claim 1, characterized in that the mass ratio of carvacrol and diconazole is 1-9:20-1. 3. The biological pesticide for preventing and treating wild rice rust according to claim 1, characterized in that the mass ratio of carvacrol and carbendazim is 1-40:15-1. 4. A pesticide preparation, characterized in that it includes the biological pesticide for preventing and treating wild rice rust described in any one of claims 1-3.