CN103969305B - bipolar electrode electrochemical luminescence imaging electrolytic cell - Google Patents

Abstract

A kind of bipolar electrode electrochemical luminescence imaging electrolytic cell, the upper surface of subtegulum stick with glue be connected in substrate, the upper surface of middle substrate sticks with glue and is connected to substrate, and upper substrate, middle substrate, subtegulum are lucite sheet.It is machined with electrolytic cell c in the center of upper substrate, the end of electrolytic cell c is middle substrate, it is machined with passage b in the center of upper substrate and middle substrate, the end of passage b is subtegulum, left end at upper substrate and middle substrate upper channel b is machined with left reservoir a, right-hand member is machined with right reservoir d, and passage b and left reservoir a and right reservoir d is connected.The present invention have reasonable in design, simple in construction, reusable, just put the advantages such as general with inverted microscope, can be applicable in bipolar electrode electrochemical luminescence imaging analysis.

Description

Bipolar Electrode Electrochemiluminescence Imaging Electrolytic Cell

technical field

The invention belongs to the technical field of electrochemiluminescence equipment or devices, and in particular relates to a field-induced electrochemiluminescence electrolytic cell utilizing the electric field enrichment effect of double electrodes.

Background technique

A bipolar electrode is a conductor not connected to an external power source but placed in the electrolyte between the driven cathode and anode. When a sufficiently strong electric field is applied to the electrolyte solution, even if the conductor is not directly electrically connected to the external power source, the Faraday reaction can occur simultaneously at both ends of the conductor, and the oxidation reaction occurs at the end close to the driving cathode, called the anode, The reduction reaction occurs near the end of the driving anode, which is called the cathode. Electrochemiluminescence is a kind of light radiation produced by applying a certain voltage on the electrode to cause a chemical reaction between the electrode reaction product or between the electrode reaction product and a certain component in the solution. Add luminescent reagents in the electrolytic cell, such as ruthenium bipyridine-tripropylamine, luminol-hydrogen peroxide, etc., and apply a voltage across the driving electrodes, and a luminescent signal will be detected at one end of the bipolar electrode. The traditional bipolar electrode is made directly on the substrate of the electrolytic cell by standard photolithography, and the channel mold is also made by standard photolithography, and then poured polydimethylsiloxane and coagulant, heat-cured, There are four steps of demolding and irreversible encapsulation with the bipolar electrode substrate to obtain the whole of the bipolar electrode and the channel sealing of the polydimethylsiloxane electrolytic cell. This method is complicated to manufacture, and because the bipolar electrodes are easily damaged, the reuse rate of the electrolytic cell channel is low.

Contents of the invention

The technical problem to be solved by the present invention is to overcome the above-mentioned shortcomings of the traditional bipolar electrode electrochemical electrolytic cell, and provide a bipolar electrode electrochemiluminescence imaging electrolytic cell with reasonable design and simple structure.

The technical solution adopted to solve the above-mentioned technical problems is: a middle substrate is arranged on the upper surface of the lower substrate, an upper substrate is arranged on the upper surface of the middle substrate, an electrolytic cell c is processed at the center of the upper substrate, and the electrolytic cell The bottom of c is the middle substrate, a channel b is processed at the center of the upper substrate and the middle substrate, the bottom of the channel b is the lower substrate, and the left end of the channel b on the upper substrate and the middle substrate is processed with a left liquid storage The groove a and the right end are processed with the right liquid storage tank d, and the channel b communicates with the left liquid storage tank a and the right liquid storage tank d.

The shape of the electrolytic cell c of the present invention is a square electrolytic cell groove or a rectangular electrolytic cell groove; the shape of the channel b is a rectangular groove, the long side length of the rectangular groove>the side length of the square groove, Length > the length of the wide side of the rectangular groove. In the present invention, the centerlines of the left liquid storage tank a, the right liquid storage tank d, and the channel b coincide with the horizontal centerline of the electrolytic cell c.

The shape of the left liquid storage tank a of the present invention is a circular groove, the diameter of the circular groove is greater than the width of the channel b, and the shape of the right liquid storage tank d is exactly the same as that of the left liquid storage tank a.

The side length of the groove of the square electrolytic cell of the present invention is 6-9mm, and the length and width of the groove of the rectangular electrolytic cell are 6×9. The channel b of the present invention has a length of 16-22 mm and a width of 2-3 mm. The diameters of the left liquid storage tank a and the right liquid storage tank d of the present invention are 3-5 mm.

The side length of the square electrolytic cell of the present invention is optimally 8.5mm. The optimal length of the channel b of the present invention is 20 mm and the width is 3 mm. The optimum diameters of the left liquid storage tank a and the right liquid storage tank d of the present invention are 4mm.

Because the electrolytic cell of the present invention and the bipolar electrode are independent of each other, they can be reused, and the bipolar electrode (such as gold, silver, etc.) In this case, the electrode faces down and does not touch the bottom of the cell, and an inverted microscope is used to detect from below, which expands the scope of application of the electrolytic cell. The invention has the advantages of reasonable design, simple structure, reusability, common upright and inverted microscopes, etc., and can be popularized and applied in electrochemiluminescence imaging analysis of bipolar electrodes.

Description of drawings

Fig. 1 is a schematic structural diagram of Embodiment 1 of the present invention.

Fig. 2 is a cross-sectional view along line A-A of Fig. 1 .

detailed description

The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments, but the present invention is not limited to the following embodiments.

Example 1

As shown in FIGS. 1 and 2 , the bipolar electrode electrochemiluminescence imaging electrolytic cell of this embodiment is composed of an upper substrate 1 , a middle substrate 2 , and a lower substrate 3 .

The upper surface of the lower substrate 3 is bonded with the middle substrate 2, the upper surface of the middle substrate 2 is bonded with the upper substrate 1, the upper substrate 1, the middle substrate 2, and the lower substrate 3 are organic glass sheet. An electrolytic cell c is processed at the center of the upper substrate 1, the structure of the electrolytic cell c is a square groove, the bottom of the square groove is the middle substrate 2, and the side length of the square groove is 8.5 mm. And the central position of substrate 2 is processed with channel b, the shape of channel b is a rectangular groove, the bottom of the rectangular groove is the lower substrate 3, the length of the rectangular groove is 20mm, and the width is 3mm. For the electrochemiluminescence reagent used for detection, the left end of the channel b on the upper substrate 1 and the middle substrate 2 is processed with a left liquid storage tank a, the right end is processed with a right liquid storage tank d, the left liquid storage tank a and the right liquid storage tank The shape of d is a circular groove with a diameter of 4mm, and the bottom of the circular groove is the lower substrate 3 . Channel b communicates with left liquid storage tank a and right liquid storage tank d respectively, and the centerlines of left liquid storage tank a, right liquid storage tank d, and channel b coincide with the horizontal centerline of electrolytic cell c, and electrolytic cell c uses The amphoteric electrode 4 is mounted on a glass substrate, which has been widely used in electrochemistry and reported in the literature.

Example 2

The structure of the electrolytic cell c is a square groove, the side length of which is 6mm, the shape of the channel b is a rectangular groove, the length of the rectangular groove is 16mm, and the width is 2mm, and the shape of the left storage tank a is circular The groove, the diameter of the circular groove is 4mm, the structure of the right reservoir d is exactly the same as that of the left reservoir a. Other components and the coupling relationship of the components are the same as in Embodiment 1.

Example 3

The structure of the electrolytic cell c is a square groove, the side length of which is 9mm, the shape of the channel b is a rectangular groove, the length of the rectangular groove is 22mm, and the width is 3mm, and the shape of the left storage tank a is circular Groove, the diameter of the circular groove is 5mm, and the structure of the right reservoir d is exactly the same as that of the left reservoir a. Other components and the coupling relationship of the components are the same as in Embodiment 1.

Example 4

In embodiment 1, the structure of the electrolytic cell c is a rectangular groove, the length of the rectangular groove is 9mm, and the width is 6mm. The centerlines of b coincide. Other components and the connection relationship of the components are the same as those in the corresponding embodiments.

Claims (5)

1. a bipolar electrode electrochemical luminescence imaging electrolytic cell, is provided with upper substrate on subtegulum (3) (1), upper substrate (1) is machined with the left storage that electrolytic cell (c), passage (b) and passage (b) are connected Liquid bath (a) and right reservoir (d), be provided with bipolar electrode 4 in electrolytic cell (c), it is characterised in that: Being provided with middle substrate (2) between upper substrate (1) and subtegulum (3), described electrolytic cell (c) is positioned at The center of substrate (1), the end of electrolytic cell (c) is middle substrate (2)；Described passage (b) processing exists Upper substrate (1) and the center of middle substrate (2), the end of passage (b) is subtegulum (3)；A described left side Reservoir (a) and right reservoir (d) are processed on upper substrate (1) and middle substrate (2), left reservoir (a) It is subtegulum (3) with the end of right reservoir (d).

Bipolar electrode electrochemical luminescence imaging electrolytic cell the most according to claim 1, it is characterised in that: Described electrolytic cell (c) be shaped as square electrolytic cell groove or rectangle electrolytic cell groove；Described is logical Road (b) be shaped as rectangular recess, the length of side of the long length of side ＞ square indentations of rectangular recess, square The broadside of the length of side ＞ rectangular recess of groove is long；Described left reservoir (a), right reservoir (d), logical The center line in road (b) coincides with the horizontal center line of electrolytic cell (c).

Bipolar electrode electrochemical luminescence imaging electrolytic cell the most according to claim 1, it is characterised in that: The groove generally circular in shape of described left reservoir (a), the width of diameter ＞ passage (b) of circular groove, right The shape of reservoir (d) is identical with the shape of left reservoir (a).

Bipolar electrode electrochemical luminescence imaging electrolytic cell the most according to claim 2, it is characterised in that: Described square electrolytic cell is recessed+and the length of side of groove is 6～9mm, rectangle electrolytic cell groove length × a width of 6 × 9； A length of the 16 of described passage (b)～22mm, a width of 2～3mm；Described left reservoir (a) and right liquid storage A diameter of the 3 of groove (d)～5mm.

Bipolar electrode electrochemical luminescence imaging electrolytic cell the most according to claim 2, it is characterised in that: The length of side of described square electrolytic cell is 8.5mm；A length of 20mm of described passage (b), a width of 3mm； Described left reservoir (a), a diameter of 4mm of right reservoir (d).