CN201016954Y - A Static Electrochemiluminescent Electrolytic Cell Suitable for Sheet Electrodes - Google Patents
A Static Electrochemiluminescent Electrolytic Cell Suitable for Sheet Electrodes Download PDFInfo
- Publication number
- CN201016954Y CN201016954Y CNU2007200064598U CN200720006459U CN201016954Y CN 201016954 Y CN201016954 Y CN 201016954Y CN U2007200064598 U CNU2007200064598 U CN U2007200064598U CN 200720006459 U CN200720006459 U CN 200720006459U CN 201016954 Y CN201016954 Y CN 201016954Y
- Authority
- CN
- China
- Prior art keywords
- electrolytic cell
- electrode
- static
- groove
- working electrode
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 230000003068 static effect Effects 0.000 title claims abstract description 17
- 210000004027 cell Anatomy 0.000 claims abstract description 48
- 210000005056 cell body Anatomy 0.000 claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 6
- 238000002347 injection Methods 0.000 claims abstract description 5
- 239000007924 injection Substances 0.000 claims abstract description 5
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims description 11
- 239000011521 glass Substances 0.000 claims description 7
- ZOMNIUBKTOKEHS-UHFFFAOYSA-L dimercury dichloride Chemical class Cl[Hg][Hg]Cl ZOMNIUBKTOKEHS-UHFFFAOYSA-L 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- -1 polytetrafluoroethylene Polymers 0.000 claims 1
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims 1
- 239000004810 polytetrafluoroethylene Substances 0.000 claims 1
- 238000002474 experimental method Methods 0.000 abstract description 6
- 230000007246 mechanism Effects 0.000 abstract description 4
- 239000000126 substance Substances 0.000 abstract description 4
- 238000005868 electrolysis reaction Methods 0.000 abstract description 3
- 238000011160 research Methods 0.000 abstract description 3
- 238000000504 luminescence detection Methods 0.000 abstract 1
- 238000001378 electrochemiluminescence detection Methods 0.000 description 15
- 238000012360 testing method Methods 0.000 description 6
- 229910052697 platinum Inorganic materials 0.000 description 5
- 238000001514 detection method Methods 0.000 description 4
- HWYHZTIRURJOHG-UHFFFAOYSA-N luminol Chemical compound O=C1NNC(=O)C2=C1C(N)=CC=C2 HWYHZTIRURJOHG-UHFFFAOYSA-N 0.000 description 4
- 230000003287 optical effect Effects 0.000 description 4
- 239000003292 glue Substances 0.000 description 3
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 229910052802 copper Inorganic materials 0.000 description 2
- 239000010949 copper Substances 0.000 description 2
- 238000002484 cyclic voltammetry Methods 0.000 description 2
- 230000005518 electrochemistry Effects 0.000 description 2
- 239000008151 electrolyte solution Substances 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
- 229910052737 gold Inorganic materials 0.000 description 2
- 239000010931 gold Substances 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 239000004215 Carbon black (E152) Substances 0.000 description 1
- 229910021607 Silver chloride Inorganic materials 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000003487 electrochemical reaction Methods 0.000 description 1
- 238000005401 electroluminescence Methods 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 230000005281 excited state Effects 0.000 description 1
- 230000005283 ground state Effects 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- AMGQUBHHOARCQH-UHFFFAOYSA-N indium;oxotin Chemical compound [In].[Sn]=O AMGQUBHHOARCQH-UHFFFAOYSA-N 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000012452 mother liquor Substances 0.000 description 1
- 229910052762 osmium Inorganic materials 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 229910052707 ruthenium Inorganic materials 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- HKZLPVFGJNLROG-UHFFFAOYSA-M silver monochloride Chemical compound [Cl-].[Ag+] HKZLPVFGJNLROG-UHFFFAOYSA-M 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Landscapes
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
一种适用于薄片电极的静态电致化学发光电解池，涉及一种电解池。提供一种实用，简便，易于更换薄片电极，可同时进行电化学实验和发光检测，以利于对一些典型发光物质的机理进行更好研究，适用于薄片电极的静态电致化学发光电解池。设有电解池槽体、T形电解池主体、工作电极、参比电极和辅助电极。电解池槽体呈长方体；T形电解池主体呈T形结构，在电解池主体底部背面设有工作电极槽，工作电极槽中设有透光孔，电解池主体背面中设有嵌槽，电解池主体中间设有3个槽，分别为参比电极槽、辅助电极槽和注液口。工作电极、参比电极和辅助电极分别设于工作电极槽、参比电极槽和辅助电极槽中。
The invention relates to a static electrochemiluminescent electrolytic cell suitable for sheet electrodes, relating to an electrolytic cell. Provides a practical, simple, easy to replace sheet electrode, which can carry out electrochemical experiments and luminescence detection at the same time, so as to facilitate better research on the mechanism of some typical luminescent substances, and is suitable for static electrochemiluminescence electrolysis cells with sheet electrodes. An electrolytic cell body, a T-shaped electrolytic cell body, a working electrode, a reference electrode and an auxiliary electrode are provided. The tank body of the electrolytic cell is a cuboid; the main body of the T-shaped electrolytic cell is a T-shaped structure. There is a working electrode tank on the back of the bottom of the main body of the electrolytic cell. There is a light-transmitting hole in the working electrode tank. There are three grooves in the middle of the main body of the pool, which are the reference electrode groove, the auxiliary electrode groove and the liquid injection port. The working electrode, the reference electrode and the auxiliary electrode are respectively arranged in the working electrode groove, the reference electrode groove and the auxiliary electrode groove.
Description
Technical field
The utility model relates to a kind of electrolytic cell, especially relates to a kind ofly detecting luminously in static systems, can be applicable to thin electrode, as the electrogenerated chemiluminescence electrolytic cell of platinized platinum, gold plaque and indium tin oxide (ITO) glass electrode etc.
Background technology
Electrogenerated chemiluminescence (electrogenerated chemiluminescence, ECL) mainly pass through electrochemical means, utilize some compound in the system to be measured in electrochemical reaction, to generate unsettled electron excitation intermediate state, when returning ground state, this material transition of excited state produces optical radiation, the light of this radiation is by behind the photomultiplier transit, by computer acquisition and record.Nineteen twenty-nine Harvey than under the electronegative potential during (2.8V) electrolysis alkalescence luminol aqueous solution first observed to have near the electrode luminescence phenomenon (Harvey E N, J.Phys.Chem., 1929,33:1456).Since mid-term in 20th century, people are to novel hydrocarbon complex (Ru, Os, transient metal complexes such as Pd) and cluster (Mo, the understanding aspect of photochemistry clusters such as W) and electrochemical properties has obtained very big progress, has meanwhile also promoted beginning to take shape of the theoretical experimental technique analytical approach of ECL.Because luminous wave spectrum of ECL and electrode potential are closely related,, can systematically study the mechanism of some typical luminescent substances therefore by writing down the variation of ECL luminous intensity (I) and electric current (i) simultaneously with electrode potential (E).
Electrolytic cell is an epochmaking part in the electrochemiluminescdetection detection system, and it mainly is made up of electrolytic cell groove and three-electrode system.Common three-electrode system is generally formed by working electrode, contrast electrode with to electrode, working electrode such as glass carbon, gold, platinum and ito glass electrode etc., and contrast electrode such as saturated calomel electrode, Ag/AgCl and filamentary silver etc. are to electrode such as platinum filament etc.
In electrochemistry experiment, the real work area of working electrode, the relative position of contrast electrode and working electrode will produce significantly influence to experimental result, and in luminescent detection system, the distance between working electrode and the optical window also influences the detection of luminous intensity.The static electrolytic cell groove of existing conventional mostly is circular, can not be well near optical window, and there is not good stationary installation to fix three-electrode system, testing measured electrode potential can not well be reappeared, thus can not with ECL spectrogram and galvanochemistry cyclic voltammetric (CV) dry straightly in conjunction with and obtain more response message.Especially slim ITO electrode in the thin electrode, because its good light transmittance, application in analytical chemistry is increasingly extensive, but because slim ITO thickness of electrode is very little, hold and can't stand pressure, therefore how to encapsulate slim ITO electrode, it is broken that it both had been difficult for, can obtain ECL spectrogram and CV figure again exactly, be the key issue of carrying out its relevant ECL research.
Summary of the invention
The purpose of this utility model is the deficiency at the existence of existing conventional electrolysis pond, a kind of practicality is provided, easy, be easy to change thin electrode, can carry out electrochemistry experiment and luminous detection simultaneously, be beneficial to the mechanism of some typical luminescent substances is better studied, be applicable to the static electrogenerated chemiluminescence electrolytic cell of thin electrode.
The utility model is provided with electrolytic cell cell body, T shape electrolytic cell main body, working electrode, contrast electrode and auxiliary electrode.The electrolytic cell cell body is rectangular parallelepiped; The T-shaped structure of T shape electrolytic cell main body, be provided with working electrode groove at the electrolytic cell bottom part body back side, be provided with light hole in the working electrode groove, the electrolytic cell main body is provided with caulking groove in the back side, be provided with 3 grooves in the middle of the electrolytic cell main body, be respectively contrast electrode groove, auxiliary electrode groove and liquid injection port.Working electrode, contrast electrode and auxiliary electrode are located at respectively in working electrode groove, contrast electrode groove and the auxiliary electrode groove.
Electrolytic cell cell body transparent surface is preferably simple glass or quartz glass, and electrolytic cell cell body side and bottom surface are preferably lead polytetrafluoroethylmaterial material side and bottom surface, so that reduce catoptrical absorption in light detects.Working electrode groove in the T shape electrolytic cell main body is preferably square groove, the degree of depth of working electrode groove is decided according to the thickness of thin slice working electrode, the light hole that the working electrode groove middle is provided with is preferably the circular hole that diameter is 2mm, its size is also decided according to needed real work electrode area, the caulking groove that is provided with in the electrolytic cell main body back side is preferably the rectangle caulking groove of wide 1cm, is used for fixing the lead that working electrode is drawn.3 grooves that are provided with in the middle of the electrolytic cell main body are preferably 3 cylindrical grooves.Working electrode is preferably thin electrode, and contrast electrode is preferably saturated calomel electrode, and auxiliary electrode is preferably platinum electrode.
Because electrolytic cell main body of the present utility model adopts T shape structure, two arms by T shape are fixed on the electrolytic cell cell body, the working electrode groove at the main body back side and caulking groove be the steady job electrode well, thereby makes the relative distance of working electrode and contrast electrode keep certain.Therefore, the utility model has the advantages that: 1) device is simple, easy operating; 2) working electrode, contrast electrode and auxiliary electrode all are well fixed, and have solved the encapsulation problem of the especially slim ITO electrode of thin electrode; 3) working electrode groove and caulking groove are easy to the replacing of electrode all in the electrolytic cell body surfaces, and practicality is wide; 4) working electrode and contrast electrode are very close and distance is certain, make the experimental data that obtains more accurate, also help the miniaturization of device simultaneously; 5) the very close optical window of working electrode has effectively reduced the loss of light signal.
Description of drawings
Fig. 1 is the structural representation of the utility model embodiment.
Fig. 2 is the front view of the utility model embodiment.
Fig. 3 is the electrolytic cell cell body front view of the utility model embodiment.
Fig. 4 is the electrolytic cell main body front view of the utility model embodiment.
Fig. 5 is the electrolytic cell main body vertical view of the utility model embodiment.
Fig. 6 is the CV figure in the utility model embodiment application.Experiment condition: electrolytic solution is the phosphate buffer (pH=7.0) that contains 0.1mol/L KBr, and luminol concentration is 10
-4Mol/L, voltage scanning speed is 0.04V/s.In Fig. 6, horizontal ordinate is current potential Potential/V vs SCE, and ordinate is electric current Curret/mA.
Fig. 7 is the ECL figure in the utility model embodiment application.In Fig. 7, horizontal ordinate is current potential Potential/V vs SCE, and ordinate is electroluminescence intensity ECL Intensity/mV.
Embodiment
Referring to Fig. 1～5, the utility model is provided with electrolytic cell cell body 3, T shape electrolytic cell main body 1, working electrode, contrast electrode and auxiliary electrode (3 electrodes in the drawings do not draw).Electrolytic cell cell body 3 is rectangular parallelepiped, and transparent surface is common or quartz glass, and side and bottom surface are the lead polytetrafluoroethylmaterial material, can reduce catoptrical absorption in light detects.T shape electrolytic cell main body 1 T-shaped structure has a square working electrode groove 4 in bottom rear, and the degree of depth of groove is decided according to the thickness of thin slice working electrode.The working electrode groove middle make a call to a diameter be the circular hole of 2mm as light hole 2, its size is also decided according to needed real work electrode area.The rectangle caulking groove 5 of a wide 1cm is cut a hole in the middle at electrolytic cell main body 1 back side, is used for fixing the lead that working electrode is drawn; In the middle of the electrolytic cell main body 1 three cylindrical grooves are arranged, be respectively contrast electrode groove 6, auxiliary electrode groove 7 and liquid injection port 8.Below provide the application example of the utility model embodiment.
1. preparation electrolytic solution: get 0.25mL 10mmol/L luminol mother liquor, be diluted to 25mL, obtain 0.1mmol/L luminol liquid to be measured with the 0.05mol/L phosphate buffer (pH=7.0) that contains 0.1mol/L KBr.
2. the installation of working electrode:
A) get a lead that thickness is suitable, remove the insulation course (each about 1cm) at its two ends, cut one section sizeable copper and be adhesive in ITO one side and the exposed end of lead, and adjust test so that its contact is good.
B) conducting surface of usefulness electroprobe test I TO glass electrode, the adhesive surface of marine glue on the one side of its conduction and the working electrode groove is sticking mutually, getting a slice length of side again is the square marine glue of 1.5cm, is bonded at the ito glass electrode back side, does not contact with water with the lead exposed part to guarantee copper glue.
C) lead is pressed into the caulking groove of electrolytic cell main body, with steady job electrode better.
3. be contrast electrode with the saturated calomel electrode, the platinum filament that a thickness is suitable is an auxiliary electrode, inserts respectively in the electrolytic cell main body corresponding electrode groove.
4. get the test fluid of 2.5mL, inject the electrolytic cell cell body, mounted electrolytic cell main body is put into,, can continue to inject test fluid to get rid of by liquid injection hole if bubble is arranged.
5. connect test circuit, open electrochemical workstation, start the cyclic voltammetry scan program, start EChrom 2000 acquisition systems simultaneously, record data.Cyclic voltammetry experiment condition: sweep speed, 0.04V/s; Sweep limit ,-1.2～1.0V.
Fig. 6 and 7 provides the result of example, has obtained ECL spectrogram and CV figure simultaneously, illustrates that the utility model can be used for the mechanism research of typical luminescent substance well in conjunction with these two kinds of spectrograms, and the result has confirmed the utlity model has above-mentioned outstanding advantage.
Claims (10)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007200064598U CN201016954Y (en) | 2007-03-02 | 2007-03-02 | A Static Electrochemiluminescent Electrolytic Cell Suitable for Sheet Electrodes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007200064598U CN201016954Y (en) | 2007-03-02 | 2007-03-02 | A Static Electrochemiluminescent Electrolytic Cell Suitable for Sheet Electrodes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201016954Y true CN201016954Y (en) | 2008-02-06 |
Family
ID=39057449
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2007200064598U Expired - Fee Related CN201016954Y (en) | 2007-03-02 | 2007-03-02 | A Static Electrochemiluminescent Electrolytic Cell Suitable for Sheet Electrodes |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201016954Y (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102749283A (en) * | 2012-07-23 | 2012-10-24 | 黑龙江大学 | Electrochromism testing device and testing method |
| CN103645225A (en) * | 2013-12-05 | 2014-03-19 | 华中科技大学 | Electrochemical testing device for organic semiconductor |
| CN103969305B (en) * | 2014-04-30 | 2016-08-17 | 陕西师范大学 | bipolar electrode electrochemical luminescence imaging electrolytic cell |
-
2007
- 2007-03-02 CN CNU2007200064598U patent/CN201016954Y/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102749283A (en) * | 2012-07-23 | 2012-10-24 | 黑龙江大学 | Electrochromism testing device and testing method |
| CN103645225A (en) * | 2013-12-05 | 2014-03-19 | 华中科技大学 | Electrochemical testing device for organic semiconductor |
| CN103969305B (en) * | 2014-04-30 | 2016-08-17 | 陕西师范大学 | bipolar electrode electrochemical luminescence imaging electrolytic cell |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Chiang et al. | Tris (2, 2′-bipyridyl) ruthenium (III)-based electrochemiluminescence detector with indium/tin oxide working electrode for capillary electrophoresis | |
| Kang et al. | Electrochemiluminescence quenching as an indirect method for detection of dopamine and epinephrine with capillary electrophoresis | |
| CN105866107B (en) | Analytical equipment based on electrogenerated chemiluminescence technology and bipolar electrode configuration | |
| CN102788831A (en) | Microfluidic chip electrophoretic-electrochemical detecting device with adjustable pH after separation and use thereof | |
| CN201016954Y (en) | A Static Electrochemiluminescent Electrolytic Cell Suitable for Sheet Electrodes | |
| CN103852502B (en) | An electrolytic cell and detection method suitable for needle-tip-enhanced Raman spectroscopy | |
| CN205643389U (en) | A compound scanning microprobe of bipolar electrode for in site measurement metal surface pH value distribution | |
| CN109001271A (en) | A kind of thin liquid layer electrochemical reaction cell suitable for In-situ Infrared detection | |
| CN106248769B (en) | The method for detecting hydroquinone concentration in solution | |
| CN103776883B (en) | A kind of preparation method of integrated microarray electrode | |
| Ji et al. | Determination of glutathione in individual Ramos cells by capillary electrophoresis with electrochemiluminescence detection | |
| Huang et al. | Determination of atenolol and metoprolol by capillary electrophoresis with tris (2, 2′-bipyridyl) ruthenium (II) electrochemiluminescence detection | |
| CN101650329A (en) | Thin-layer long optical distance spectrum electrochemical pool with capillary interface | |
| CN104614422A (en) | Electrochemical reaction device and method for bipolar electrode coupling | |
| Sreedhar et al. | Determination of tertiary amines based on pH junctions and field amplification in capillary electrophoresis with electrochemiluminescence detection | |
| AU2004250141A1 (en) | Electrochemiluminescence electrode | |
| CN201007715Y (en) | Flow system electrochemiluminescence electrolysis cell for indium tin oxide glass electrode | |
| CN102520162A (en) | Method for detecting glycoprotein | |
| CN104132976A (en) | Method for in-situ construction of electrode through electro-depositing super-stable metal thin films on ITO conductive glass surface | |
| CN113376230A (en) | Photoelectrochemical optical fiber microelectrode adopting electrode internal illumination mode and preparation method thereof | |
| CN105806833A (en) | Electrochemical luminescence detection pool | |
| CN205719989U (en) | A kind of electrochemical luminescence detecting pool | |
| Hu et al. | Cathodic electrochemiluminescence in aqueous solutions at bismuth electrodes | |
| CN202083620U (en) | A Flow Injection Electrochemiluminescence Analysis Device | |
| CN108152210B (en) | A special electrochemiluminescence detection cell for screen printing electrodes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20080206 Termination date: 20110302 |