CN204514853U - A kind of simple and easy peripheral blood lymphocyte electrophoretic apparatus - Google Patents

Abstract

A simple electrophoresis device for peripheral blood lymphocytes, characterized in that it includes a blood cell counting board and a cover glass, a counting platform with counting grids on the upper surface is formed on the blood counting board, and the two sides of the counting platform are respectively formed to connect with the counting platform The first groove and the second groove are connected, and the outer sides of the first groove and the second groove form a cover glass support platform, and the cover glass is placed on the cover glass support platform and forms a counting pool with the counting platform A first electrode is installed in the first groove, a second electrode is installed in the second groove, and electrode leads are connected to the first electrode and the second electrode. The utility model has the advantages that: the electrophoresis device has a simple structure and is easy to manufacture. When in use, after diluting the separated lymphocytes with physiological saline, they are dropped into the counting pool and an electric field is added, and a certain lymphocyte can be specifically observed under a microscope. It is very convenient to use the number of counting grids that cells swim through in a certain period of time.

Description

A Simple Peripheral Blood Lymphocyte Electrophoresis Device

technical field

The utility model relates to an electrophoresis device, in particular to a simple peripheral blood lymphocyte electrophoresis device.

Background technique

Electrophoresis is the movement of charged particles or molecules in an electric field. Under electric field conditions, negatively charged molecules move toward the anode, and positively charged molecules move toward the cathode. Under the action of unit electric field strength, the distance that charged particles move in unit time (ie, mobility) is a constant. , reflecting the physicochemical characteristics of the charged particle. Different charged particles are separated from each other due to different moving distances after a period of electrophoresis in the same electric field due to different charges, or the same charge but different charge-to-mass ratios. The separation distance is related to the voltage of the applied electric field and the electrophoresis Time is proportional. This technology that uses electrophoresis to separate substances is called electrophoresis. Most biological macromolecules such as proteins, nucleic acids, polysaccharides, etc. have cationic particles and anionic groups, which can be separated by electrophoresis.

The instruments needed for electrophoresis mainly include power supply and electrophoresis tank. To make charged biomacromolecules swim in an electric field, an external electric field must be applied, and the resolution and speed of electrophoresis are closely related to the electrical parameters during electrophoresis. The electrophoresis tank is the core component of the electrophoresis system. According to the principle of electrophoresis, the electrophoresis support is placed between the two electrode buffers, and the electric field connects the two buffers through the electrophoresis support. Different electrophoresis methods use different electrophoresis tanks.

At present, there are various electrophoresis devices with different structures in the prior art, such as the "reversible current coagulation for separating biological macromolecules" disclosed in the Chinese invention patent application with the application number 201080049281.X (the application publication number is CN 102656449 U). "Gel electrophoresis device", another example is the "electrophoresis device" disclosed in the Chinese invention patent application whose application number is 01136660.5 (publication number is CN 1350179 A). The main defect of the above-mentioned electrophoresis device is that the structure is relatively complicated and the production cost is relatively high.

Utility model content

The technical problem to be solved by the utility model is to provide a simple peripheral blood lymphocyte electrophoresis device with simple structure, low cost and convenient use, aiming at the above-mentioned current state of the art.

The technical scheme adopted by the utility model to solve the above technical problems is: the simple peripheral blood lymphocyte electrophoresis device, which is characterized in that it includes a blood cell counting board and a cover glass, and an upper surface is formed on the blood counting board. A grid counting platform, the two sides of the counting platform are respectively formed with a first groove and a second groove communicating with the counting platform, and the outer sides of the first groove and the second groove form a cover glass supporting platform , the cover glass is placed on the cover glass supporting platform and forms a counting pool with the counting platform, the first electrode is installed in the first groove, and the A second electrode is installed in the second groove, and electrode leads are connected to the first electrode and the second electrode.

In order to allow the cell solution to drop smoothly into the counting cuvette, preferably, the gap between the cover glass and the counting platform forms the counting cuvette, and the size of the gap is 0.05mm-0.2mm.

Further preferably, the size of the gap is 0.1mm. After the gap of the counting pool adopts this size, it is more conducive to the smooth dripping and counting of lymphocytes.

Further preferably, the counting platform is square, the first groove and the second groove are parallel to each other, the first electrode is arranged along the length direction of the first groove, and the second electrode is arranged along the length direction of the first groove. set along the length direction of the second groove. In this way, the structure of the whole electrophoresis device is simpler and the manufacture is more convenient.

The first electrode and the second electrode can have many different installation structures. Preferably, the first electrode and the second electrode are platinum electrode wires, and are respectively bonded and fixed on both sides of the counting platform.

In order to fix the electrode leads, fixing screws are provided on the electrode leads of the first electrode and the second electrode.

In order to prevent the cell solution dripping into the counting tank from flowing out, a horizontal bar is provided on the counting platform, and the two ends of the transverse bar extend to the first groove and the second groove respectively.

As a preference for any of the above solutions, strip-shaped grooves are respectively formed on the outside of the cover glass supporting platform, and the length direction of the strip-shaped grooves is parallel to the first groove and the second groove the length direction.

Compared with the prior art, the utility model has the advantages that: the electrophoresis device is provided with a counting platform with counting grids on the upper surface on the blood cell counting plate, grooves are formed on both sides of the counting platform, electrodes are placed in the grooves, and the electrodes lead out The electrode leads and the cover glass are covered on the supporting platform of the counting plate and form a counting pool between the counting platform. The whole set of device is simple in structure and easy to manufacture. When in use, the isolated lymphocytes are diluted with normal saline and then dropped into the counting pool. With an electric field added to the pool, the number of counting grids that a certain lymphocyte swims through within a certain period of time can be observed under a microscope, which is very convenient to use.

Description of drawings

Fig. 1 is the side view of hemocytometer plate and cover glass in the utility model embodiment;

Fig. 2 is the side view of the utility model embodiment;

Fig. 3 is the enlarged schematic view of part A in Fig. 2;

Fig. 4 is the top view of the utility model embodiment (remove the cover glass).

Detailed ways

The utility model is described in further detail below in conjunction with the accompanying drawings.

As shown in Figures 1 to 4, the simple peripheral blood lymphocyte electrophoresis device in this embodiment includes a hemocytometer 1 and a cover glass 2. The upper surface of the hemocytometer 1 is rectangular and forms a The square counting platform 3 has counting grids on the upper surface of the counting platform 3. Since the counting grids cannot be seen with the naked eye but can only be seen under a microscope, the counting grids are not shown in the figure. The two sides of counting platform 3 form the first groove 41 and the second groove 42 that communicate with counting platform 3, and the first groove 41 and the second groove 42 are parallel to each other, and the first groove 41 and the second groove The longitudinal direction of the groove 42 is consistent with the width direction of the blood cell counter 1 .

A cover glass support platform 5 is also formed on the upper surface of the hemocytometer 1 , specifically, the cover glass support platform 5 is formed outside the first groove 41 and the second groove 42 . The cover glass 2 is placed on the cover glass support platform 5 and forms a counting pool 6 with the counting platform 3, that is, the gap between the cover glass 2 and the counting platform 3 forms the counting pool 6. In this embodiment, the gap The size is 0.1mm.

The first electrode 71 is installed in the first groove 41, the second electrode 72 is installed in the second groove 42, and the first electrode 71 is arranged along the length direction of the first groove 41, and the second electrode 72 is arranged along the second groove 41. The lengthwise direction of the two grooves 42 is arranged, that is, the first electrode 71 and the second electrode 72 are also arranged parallel to each other. Electrode lead wires 8 are connected to the first electrode 71 and the second electrode 72 , and fixing screws 9 are provided on the electrode lead wires 8 of the first electrode 71 and the second electrode 72 . In this embodiment, both the first electrode 71 and the second electrode 72 are platinum electrode wires with a square cross-section, and are glued and fixed on both sides of the counting platform 3 respectively.

In order to prevent the cell solution dripping into the counting tank from flowing out, a horizontal bar 10 is arranged on the counting platform 3 , and the two ends of the bar 10 extend to the first groove 41 and the second groove 42 respectively. In addition, in this embodiment, strip-shaped grooves 11 are respectively formed on the outside of the cover glass supporting platform 5 , and the length direction of the strip-shaped grooves 11 is parallel to the length directions of the first groove 41 and the second groove 42 .

When in use, separate lymphocytes, dilute them with normal saline, drop them into the counting pool, apply an electric field, and observe the number of counting grids that a certain lymphocyte swims through within a certain period of time under a microscope, which is very convenient to use.

The above descriptions are only preferred implementations of the present utility model. It should be pointed out that for those of ordinary skill in the art, various modifications or improvements can be made to the present utility model without departing from the principle of the present utility model. It is considered within the protection scope of the present utility model.

Claims (8)

1. a simple and easy peripheral blood lymphocyte electrophoretic apparatus, it is characterized in that: comprise blood counting chamber (1) and cover glass (2), described blood counting chamber (1) is formed the counting platform (3) of upper surface band counting grid, the both sides of described counting platform (3) are formed with the first groove (41) and the second groove (42) that are connected with counting platform respectively, the outside of described first groove (41) and the second groove (42) forms cover glass support platform (5), described cover glass (2) be placed on described cover glass support platform (5) upper and and form counting chamber (6) between described counting platform (3), in described the first groove (41), the first electrode (71) is installed, in described the second groove (42), the second electrode (72) is installed, described the first electrode (71) and the second electrode (72) are all connected with contact conductor (8).

2. simple and easy peripheral blood lymphocyte electrophoretic apparatus according to claim 1, it is characterized in that: the gap between described cover glass (2) and counting platform (3) forms described counting chamber (6), and the size in described gap is 0.05mm ~ 0.2mm.

3. simple and easy peripheral blood lymphocyte electrophoretic apparatus according to claim 2, is characterized in that: the size in described gap is 0.1mm.

4. simple and easy peripheral blood lymphocyte electrophoretic apparatus according to claim 1, it is characterized in that: described counting platform (3) is square, described the first groove (41) and the second groove (42) are parallel to each other, described the first electrode (71) is arranged along the length direction of the first groove (41), and described the second electrode (72) is arranged along the length direction of the second groove (42).

5. simple and easy peripheral blood lymphocyte electrophoretic apparatus according to claim 1, it is characterized in that: described the first electrode (71) and the second electrode (72) are platinum electrode silk, and be adhesively fixed on the both sides of described counting platform (3) respectively.

6. simple and easy peripheral blood lymphocyte electrophoretic apparatus according to claim 1, is characterized in that: on the contact conductor (8) of described first electrode (71) and the second electrode (72), be equipped with fixed screw (9).

7. simple and easy peripheral blood lymphocyte electrophoretic apparatus according to claim 1, it is characterized in that: on described counting platform (3), be provided with a horizontal blend stop (10), and the two ends of described horizontal blend stop (10) extend to described the first groove (41) and the second groove (42) place respectively.

8. the simple and easy peripheral blood lymphocyte electrophoretic apparatus according to claim arbitrary in claim 1 to 7, it is characterized in that: be formed with strip groove (11) respectively in the outside of described cover glass support platform (5), and the length direction of described strip groove (11) is parallel to described the first groove (41) and the length direction of the second groove (42).