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CN103141447B - A method for artificially cultivating crickets without soil temperature control - Google Patents

A method for artificially cultivating crickets without soil temperature control Download PDF

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CN103141447B
CN103141447B CN201310101322.0A CN201310101322A CN103141447B CN 103141447 B CN103141447 B CN 103141447B CN 201310101322 A CN201310101322 A CN 201310101322A CN 103141447 B CN103141447 B CN 103141447B
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杨俊宴
朱建军
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Southeast University
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Abstract

本发明提供了一种无土温控人工培养蟋蟀的方法,包括野生蟋蟀受精籽的冻籽处理、野生蟋蟀受精籽的恒温培养、蟋蟀1-4蜕若虫的变温培养、蟋蟀5-6蜕大龄若虫的分缸培养。该发明方法工艺简单、设备简单、成本低廉,采用完全无土的蟋蟀人工培育方法,具有明显的全天候反季节性,避免了各种土壤病菌致病因素,产率高;同时,使高品系的蟋蟀人工培养更具稳定性和准确性,培育的蟋蟀个头大、品系好。The invention provides a method for artificially cultivating crickets without soil temperature control, including freezing seed treatment of fertilized seeds of wild crickets, constant temperature cultivation of fertilized seeds of wild crickets, variable temperature cultivation of 1-4 molting nymphs of crickets, 5-6 molting nymphs of crickets Cylinder culture of nymphs. The inventive method is simple in process, simple in equipment and low in cost, adopts a completely soilless cricket artificial cultivation method, has obvious all-weather anti-seasonality, avoids various soil pathogenic factors, and has high yield; Artificial cultivation of crickets is more stable and accurate, and the cultivated crickets are large in size and good in strain.

Description

一种无土温控人工培养蟋蟀的方法A method for artificially cultivating crickets without soil temperature control

技术领域technical field

本发明属于昆虫人工繁殖技术领域,特别涉及一种无土温控环境下人工培养蟋蟀的方法。The invention belongs to the technical field of insect artificial propagation, in particular to a method for artificially cultivating crickets in a soilless temperature-controlled environment.

背景技术Background technique

蟋蟀也称中华斗蟋,为直翅目昆虫,斗蟋蟀是中国一项传统的文化娱乐活动,高品系的蟋蟀具有很高的经济价值。然而,自然环境下蟋蟀的寿命仅为100天左右,成虫时间也限定在秋天;同时,山东等传统野生蟋蟀产地资源濒临枯竭,如何人工培养蟋蟀,保护自然种群,沿承蟋蟀传统文化,是近代长期研究的一个课题。Crickets, also known as Chinese crickets, are insects of the order Orthoptera. Fighting crickets is a traditional cultural and recreational activity in China. High-grade crickets have high economic value. However, the lifespan of crickets in the natural environment is only about 100 days, and the adult time is also limited to autumn. At the same time, resources in traditional wild cricket producing areas such as Shandong are on the verge of exhaustion. How to artificially cultivate crickets to protect natural populations and inherit traditional cricket culture is a modern A subject of long-term research.

1980年代以来,关于人工培养蟋蟀的实验研究逐渐兴起,概括起来大致有二种培养方向:一是室外撒籽自然培养,在冬天选择合适的田地圈围,在浅土层撒蟋蟀籽使其自然生长;二是室内人工培养,选择室内环境恒温培养蟋蟀。Since the 1980s, experimental research on artificial cultivation of crickets has gradually emerged. In general, there are roughly two cultivation directions: one is to sow seeds outdoors for natural cultivation, and to choose suitable field enclosures in winter, and to spread cricket seeds in shallow soil layers to make them grow naturally. Growth; The second is indoor artificial cultivation, selecting indoor environment constant temperature to cultivate crickets.

然而,现有人工方法培养出来的蟋蟀产率低、个头小、品系低,打斗能力远远不如野生蟋蟀,其主要技术缺陷为:However, the crickets cultivated by existing artificial methods have low yield, small size and low strain, and their fighting ability is far inferior to that of wild crickets. The main technical defects are:

1.产率低。无论哪种培养方法,均采用土壤环境,使得土壤中大量病菌和病毒在高温环境下迅速生长繁殖,导致蟋蟀病死率较高。1. Low yield. Regardless of the cultivation method, the soil environment is adopted, so that a large number of germs and viruses in the soil can grow and reproduce rapidly in a high-temperature environment, resulting in a higher mortality rate of crickets.

2.品系低。因此野生蟋蟀籽在野外自然环境下经历整个冬天的低温环境,对其线粒体沉淀和品系稳定具有十分重要的作用,然而现有人工培养方法缺少模拟蟋蟀自然孵化过程中的关键步骤--冻籽,因此导致人工培养出的蟋蟀品系较低。2. Strain is low. Therefore, wild cricket seeds experience the low temperature environment throughout the winter in the natural environment in the wild, which plays a very important role in the precipitation of mitochondria and strain stability. However, the existing artificial cultivation methods lack the key step of simulating the natural hatching process of crickets - freezing seeds. Therefore, the artificially cultivated cricket strains are lower.

3.个头小、品系低。由于培育全程均为自然温度或者人工恒温,导致缺乏适应蟋蟀自然发育特征的温湿度环境条件,从蟋蟀籽孵化到7龄蜕皮成虫全过程均缺乏精确的温湿度控制,这种物理环境并不适合蟋蟀的生长发育需求,导致人工培养出的蟋蟀品个头小、系较低。3. Small in size and low in strain. Since the whole process of cultivation is at natural temperature or artificial constant temperature, it lacks the temperature and humidity environment conditions suitable for the natural development characteristics of crickets. The whole process from the hatching of crickets seeds to the molting adults of the 7th instar lacks precise temperature and humidity control. This physical environment is not suitable. The growth and development needs of crickets lead to the small size and lower lines of the artificially cultivated crickets.

发明内容Contents of the invention

发明目的:本发明目的在于提供一种产率高、蟋蟀个头大、品系高的无土温控人工培养蟋蟀的方法。Purpose of the invention: the purpose of the invention is to provide a method for artificially cultivating crickets without soil temperature control with high yield, large cricket size and high strain.

技术方案:本发明提供的一种无土温控人工培养蟋蟀的方法,包括以下步骤:Technical solution: A method for artificially cultivating crickets without soil temperature control provided by the invention comprises the following steps:

(1)野生蟋蟀受精籽的冻籽处理:野生蟋蟀受精籽与干沙土混匀后置于温度2-10℃中经冻籽处理100天以上;(1) Freeze seed treatment of fertilized seeds of wild crickets: mix fertilized seeds of wild crickets with dry sand and place them at a temperature of 2-10°C for more than 100 days;

(2)野生蟋蟀受精籽的恒温培养:将经冻籽处理后的野生蟋蟀受精籽与干沙土混合物于自然温度下培养1-2天后,于灭菌后的培养缸中恒温保湿培养6-7天,培养条件为:温度25-30℃,湿度80-90%;(2) Constant temperature cultivation of fertilized seeds of wild crickets: After the mixture of fertilized seeds of wild crickets treated with frozen seeds and dry sand is cultivated at natural temperature for 1-2 days, they are cultured in a sterilized cultivation tank at constant temperature and humidity for 6-7 days. days, the culture conditions are: temperature 25-30 ℃, humidity 80-90%;

(3)蟋蟀1-4蜕若虫的变温培养:于培养缸中设置2-3层斜搭桥砌作为各龄蟋蟀若虫的活动场地,将恒温培养后的野生蟋蟀受精籽于日夜变温环境中培养40-45天,培养条件为:白天温度为25-28℃、湿度为30-50%,夜间温度为30-32℃、湿度为80-90%,饲料包括对虾养殖饲料、玉米、豆皮和蝉蜕的混合物以及白菜叶;(3) Varying temperature cultivation of cricket 1-4 moulting nymphs: set 2-3 layers of inclined bridges in the cultivation tank as the activity site for cricket nymphs of all ages, and cultivate the fertilized seeds of wild crickets cultivated at constant temperature in a day and night temperature-changing environment for 40 days. -45 days, culture conditions are: daytime temperature 25-28°C, humidity 30-50%, night temperature 30-32°C, humidity 80-90%, feed includes prawn breeding feed, corn, soybean skin and cicada slough mixture and cabbage leaves;

(4)蟋蟀5-6蜕大龄若虫的分缸培养:若虫达到5蜕后分缸培养,平均1缸分成10缸,培养条件为:白天温度为25-28℃、湿度为30-50%,夜间温度为30-32℃、湿度为80-90%,饲料以对虾养殖饲料、玉米、豆皮和蝉蜕的混合物以及白菜叶。(4) Cricket 5-6 molting older nymphs are cultured in separate tanks: after the nymphs reach 5 moults, they are divided into tanks, and an average of 1 tank is divided into 10 tanks. The culture conditions are: daytime temperature is 25-28 ° C, humidity is 30-50%, The temperature at night is 30-32°C, the humidity is 80-90%, and the feed is a mixture of prawn breeding feed, corn, bean skin and cicada slough, and cabbage leaves.

其中,步骤(1)中,野生蟋蟀受精籽的保存采用无菌纸袋;从而保证了野生蟋蟀受精籽避免被其他病菌和病毒感染,提高了蟋蟀产率。Wherein, in the step (1), the fertilized seeds of wild crickets are stored in aseptic paper bags; thereby ensuring that the fertilized seeds of wild crickets are prevented from being infected by other germs and viruses, and the yield of crickets is improved.

其中,步骤(1)中,所述野生蟋蟀受精籽标样为经过质量百分比5-6‰高锰酸钾水溶液杀菌处理的受精籽;从而保证了野生蟋蟀受精籽避免被其他病菌和病毒感染,提高了蟋蟀产率。Wherein, in step (1), the wild cricket fertilized seed standard sample is the fertilized seed that has been sterilized by a mass percentage of 5-6‰ potassium permanganate aqueous solution; thereby ensuring that the wild cricket fertilized seed is prevented from being infected by other germs and viruses, Increased cricket yield.

其中,步骤(2)中,培养缸的灭菌方法为将培养缸置于温度为50-60℃、湿度为10%以下的环境中处理8-10h;从而保证了野生蟋蟀受精籽避免被其他病菌和病毒感染,提高了蟋蟀产率。Among them, in step (2), the method of sterilizing the culture tank is to place the culture tank in an environment with a temperature of 50-60°C and a humidity below 10% for 8-10 hours; thereby ensuring that the fertilized seeds of wild crickets are prevented from being fertilized by other Bacterial and viral infections increase cricket productivity.

其中,步骤(3)中,饲料中虾养殖饲料、玉米、豆皮和蝉蜕的质量比为(25-35):(30-40):(25-35):5,优选地为30:35:30:5;从而保证了蟋蟀1-4蜕若虫的营养,提高了蟋蟀的品系。Wherein, in step (3), the mass ratio of shrimp breeding feed, corn, soybean skin and cicada slough in the feed is (25-35): (30-40): (25-35): 5, preferably 30:35 :30:5; thereby ensuring the nutrition of the cricket 1-4 molting nymphs, and improving the strain of the cricket.

有益效果:本发明提供的人工培养蟋蟀的方法,工艺简单、设备简单、成本低廉,采用完全无土的蟋蟀人工培育方法,具有明显的全天候反季节性,避免了各种土壤病菌致病因素,产率高;同时,使高品系的蟋蟀人工培养更具稳定性和准确性,培育的蟋蟀个头大、品系好。Beneficial effects: the method for artificially cultivating crickets provided by the present invention has simple process, simple equipment and low cost, adopts a completely soilless artificial cultivation method for crickets, has obvious all-weather anti-seasonality, and avoids various soil pathogenic factors, The yield is high; at the same time, the artificial cultivation of high strains of crickets is more stable and accurate, and the cultivated crickets are large in size and good in strains.

具体实施方式Detailed ways

根据下述实施例,可以更好地理解本发明。然而,本领域的技术人员容易理解,实施例所描述的具体的物料配比、工艺条件及其结果仅用于说明本发明,而不应当也不会限制权利要求书中所详细描述的本发明。The present invention can be better understood from the following examples. However, those skilled in the art will readily understand that the specific material ratios, process conditions and results described in the examples are only used to illustrate the present invention, and should not and will not limit the present invention described in detail in the claims .

实施例1Example 1

无土温控人工培养蟋蟀的方法,包括以下步骤:The method for artificially cultivating crickets without soil temperature control comprises the following steps:

1.野生蟋蟀受精籽的冻籽处理1. Frozen seed treatment of wild cricket fertilized seeds

(1)野生蟋蟀受精籽的获得:将野生蟋蟀育种专用的细沙预先采用80目的筛子细筛成干沙土颗粒,在野生母本扎籽后,放置6-7天左右,使蟋蟀籽发育膨胀,发育状态连续观察如下:(1) Obtaining the fertilized seeds of wild crickets: the fine sand specially used for wild cricket breeding is sieved into dry sand particles with an 80-mesh sieve in advance. , the developmental state is continuously observed as follows:

1-3天籽呈现半透明状,开始萌发;1-3 days, the seeds are translucent and begin to germinate;

5-10天开始颜色变白,体积膨胀;After 5-10 days, the color turns white and the volume expands;

20天左右每颗籽体积大于80目。In about 20 days, the volume of each seed is greater than 80 mesh.

将采集的野生蟋蟀受精籽与细沙的混合标样以80目的筛子细筛,得到沙土分离的野生蟋蟀受精籽,用火焰灭菌的镊子和解剖针挑入无菌纸袋放置。The collected wild cricket fertilized seeds and fine sand mixed standard samples were finely sieved with an 80-mesh sieve to obtain wild cricket fertilized seeds separated from sand and soil, and were picked into sterile paper bags with flame-sterilized forceps and dissecting needles.

(2)野生蟋蟀受精籽的的灭菌处理(2) Sterilization of fertilized seeds of wild crickets

将野生蟋蟀受精籽的以质量百分比为5‰的高锰酸钾水溶液进行杀菌处理,由于野生蟋蟀受精籽的表面有不溶于水的蜡质层,使用木质牙签搅拌使其下沉入水,以充分消毒杀菌处理。The fertilized seeds of wild crickets are sterilized with a potassium permanganate aqueous solution of 5 ‰ by mass percentage. Since the fertilized seeds of wild crickets have a water-insoluble waxy layer, use a wooden toothpick to stir and make them sink into the water to Fully disinfect and sterilize.

(3)野生蟋蟀受精籽的冻籽处理(3) Freezing seed treatment of wild cricket fertilized seeds

将100℃以上火烤、自然冷却后的干沙土与灭菌处理后的野生蟋蟀受精籽混合,保持混合物总体湿度低于5%、捏手不能成团的状态,并将干沙土野生蟋蟀受精籽的混合物放入保鲜袋密封,置于2-10℃的低温环境中100天以上,充分模拟野生籽过冬环境;可根据孵化时间确定保存温度,计划100天-150天就要孵化的籽温度控制在100℃,计划1年以上才要孵化的籽温度控制在2℃左右;这期间自然死亡的籽约为5%左右,呈现明显的蜡白干瘪状。Mix the dry sand that has been baked at a temperature above 100°C and naturally cooled with the sterilized wild cricket fertilized seeds, keep the overall humidity of the mixture below 5%, and keep the mixture in a state that cannot form agglomerates when kneading hands, and dry the fertilized wild cricket seeds on the dry sand soil Put the mixture into a fresh-keeping bag and seal it, and place it in a low temperature environment of 2-10°C for more than 100 days, fully simulating the winter environment of wild seeds; the storage temperature can be determined according to the incubation time, and the temperature of the seeds that will be hatched in 100-150 days is planned to be controlled At 100°C, the temperature of the seeds that are planned to hatch for more than one year is controlled at about 2°C; during this period, about 5% of the seeds died naturally, showing an obvious waxy white and shriveled shape.

2.无土恒温孵化2. Soilless constant temperature incubation

(1)培养环境的准备(1) Preparation of the culture environment

人工培养房采用10cm厚的夹心泡沫板贴墙设置作为保温房,排列放置孵化架,架上统一铺置长40cm、宽30cm、高30cm的泡沫保温箱作为无土培育蟋蟀的培养缸,缸口敞开,上壁贴上5cm宽的光滑胶带,防止蟋蟀若虫外逃。The artificial culture room adopts 10cm thick sandwich foam boards to stick to the wall and set it as the heat preservation room. The hatching racks are arranged in a row, and the foam incubators with a length of 40 cm, a width of 30 cm and a height of 30 cm are uniformly laid on the racks as a cultivation tank for cultivating crickets without soil. Open it, and paste a 5cm wide smooth tape on the upper wall to prevent the cricket nymph from escaping.

培养缸的恒温恒湿无菌处理。蟋蟀致病源主要为螨虫、寄生蜂、多角体病毒和带病基因遗传。将培养缸预先使用电热暖风加热器组合带插座的温度控制器进行50-60℃高温杀菌、同时使用加湿器组合带插座的湿度控制器进行10%以内的低湿度杀菌,采用该方法持续处理10小时能够有效杀灭蟋蟀致病细菌和病毒。Constant temperature and humidity aseptic treatment of the culture tank. The pathogenic sources of crickets are mainly mites, parasitic wasps, polyhedrosis virus and disease-carrying genetic inheritance. Use the electric warm air heater combined with a temperature controller with a socket to carry out high-temperature sterilization at 50-60°C in advance, and at the same time use a humidifier combined with a humidity controller with a socket to carry out low-humidity sterilization within 10%. This method is used for continuous treatment 10 hours can effectively kill cricket pathogenic bacteria and viruses.

(2)野生蟋蟀受精籽的恒温培养(2) Constant temperature cultivation of fertilized seeds of wild crickets

经冻籽处理后的野生蟋蟀受精籽和干沙土混合物在室内自然温度下放置2天,喷洒水雾使湿度达到60%左右,搅拌后捏手可成团,搅拌后可散开,将铺在不锈钢的孵化盘(尺寸为20*30)上,铺设厚度在2-3cm,置于培养缸中恒温保湿培养6天,控制室温25℃,湿度85%。The fertilized seeds of wild crickets treated with frozen seeds and the mixture of dry sand and soil are placed at the natural temperature of the room for 2 days, sprayed with water mist to make the humidity reach about 60%. On the stainless steel incubator tray (size 20*30), the laying thickness is 2-3cm, placed in the culture tank for constant temperature and moisture cultivation for 6 days, the room temperature is controlled at 25°C, and the humidity is 85%.

为了提高蟋蟀出籽率,混合蟋蟀籽的细沙铺设厚度不能高于3cm,以保证所有籽的均匀受热,这样出籽率在90%以上,而且能够6-7天以内同步孵化。In order to improve the seed yield of crickets, the thickness of the fine sand mixed with cricket seeds should not be higher than 3cm to ensure that all seeds are evenly heated, so that the seed yield rate is above 90%, and they can be hatched simultaneously within 6-7 days.

其中,需要注意的事项:Among them, matters needing attention:

——期间若喷洒冷水雾,将导致60%以上孵化的热籽受凉死亡。——If cold water mist is sprayed during this period, more than 60% of the hatched hot seeds will die from the cold.

——孵化期内不可失水(籽会干瘪),也不可湿度过高(籽会窒息而死并发霉),否则蟋蟀籽将全部死亡。- During the incubation period there must be no loss of water (seeds will dry out) nor excessive humidity (seeds will suffocate and mold), otherwise all cricket seeds will die.

——孵化期内不可受到光线直射,否则籽会发红死亡。——Do not expose to direct light during the hatching period, otherwise the seeds will turn red and die.

——混合蟋蟀籽的细沙铺设厚度高于3cm,将导致过厚的蟋蟀籽上热下冷,上层籽提前孵化,而下层籽延后孵化,孵化时间相差过大,导致后孵化的小龄若虫被大龄若虫吃掉。——The thickness of the fine sand mixed with cricket seeds is more than 3cm, which will cause the top layer of cricket seeds to be hot and the bottom layer to be cold. The upper layer of seeds will hatch earlier, while the lower layer of seeds will hatch later. Nymphs are eaten by older nymphs.

3.蟋蟀1-4蜕若虫的变温培养3. Variable temperature culture of cricket 1-4 molting nymphs

以内径3*3毫米的多孔PVC扣板条(6cm*20cm)斜搭桥砌作为各龄蟋蟀若虫的活动场地,在保温箱内堆叠2-3层。贴合蟋蟀昼伏夜出的生物特性,白天采用低室温25℃、低湿度40%,有利于蟋蟀若虫休眠;夜晚采用高室温30℃、高湿度85%,促进蟋蟀若虫夜晚进食和活动发育。饲料以质量百分比为30:35:30:5的对虾养殖饲料、玉米、豆皮和蝉蜕,搅拌成颗粒状干粉,洒在缸内,内含野生环境下蟋蟀能够从土壤环境中所摄取的蛋白质、钙、电解多维等微量元素。同时将白菜叶切成1mm*1mm的碎末,撒在表层,供若虫食用补充自然养分。饮水为长10cm,宽20cm的浅水盆上盖湿布,可以有效避免蟋蟀若虫落水致死。The porous PVC gusset strips (6cm*20cm) with an inner diameter of 3*3mm are used as the activity site for cricket nymphs of all ages, and 2-3 layers are stacked in the incubator. In line with the biological characteristics of nocturnal crickets, a low room temperature of 25°C and a low humidity of 40% during the day is conducive to the dormancy of cricket nymphs; a high room temperature of 30°C and a high humidity of 85% at night is used to promote night eating and activity development of cricket nymphs. The feed is 30:35:30:5 mass percentage of prawn breeding feed, corn, bean skin and cicada slough, stirred into granular dry powder, sprinkled in the tank, containing the protein that crickets can ingest from the soil environment in the wild environment , calcium, electrolytic multidimensional and other trace elements. At the same time, cut the cabbage leaves into 1mm*1mm pieces, sprinkle them on the surface, and feed the nymphs to supplement natural nutrients. Drinking water is a shallow water basin with a length of 10 cm and a width of 20 cm, covered with a wet cloth, which can effectively prevent the death of cricket nymphs from falling into the water.

4.蟋蟀5-6蜕大龄若虫的分缸培养4. Cricket culture of 5-6 molting nymphs in separate tanks

生长40天以后,若虫达到5蜕左右,开始分缸,平均一缸分成10缸。分缸过程中,小孔板换成内径1*1cm的多孔PVC扣板条(6cm*20cm)纵横搭桥砖砌作为各龄蟋蟀若虫的活动场地,在保温箱内堆叠2-3层。另外以加热调成糊状的湿饲料抹在白菜叶上作为辅助饲料,给蜕皮后不久的嫩皮蟋蟀若虫食用,其余环境参数与步骤3一致。After 40 days of growth, the nymphs reach about 5 sloughs and start to divide into tanks, with an average of 10 tanks per tank. During the tank division process, the small orifice plate was replaced with porous PVC gusset strips (6cm*20cm) with an inner diameter of 1*1cm, and the vertical and horizontal bridging bricks were used as the activity site for cricket nymphs of all ages, and 2-3 layers were stacked in the incubator. In addition, the wet feed adjusted to paste by heating is used as auxiliary feed on the cabbage leaves, and it is eaten by the tender skin cricket nymphs shortly after molting, and the rest of the environmental parameters are consistent with step 3.

培养结果显示,通过无土温度控制方法人工培养的蟋蟀,具有以下特点:The cultivation results show that the crickets artificially cultivated by the soilless temperature control method have the following characteristics:

——成虫个头大,皮色好。经过人工优化选育和分缸培养,蟋蟀普遍个体成长度高,平均重量在60毫克以上。——The adults are big and have good skin color. After artificially optimized breeding and sub-tank cultivation, crickets generally have a high individual growth rate, with an average weight of more than 60 mg.

——成活率高。由于人工培养解决了蟋蟀自然天敌的侵害,无土环境又极大地降低了各类致病菌的侵害,使得人工培养的蟋蟀成活率达到了90%以上。——High survival rate. Because the artificial cultivation solves the invasion of crickets' natural enemies, and the soilless environment greatly reduces the invasion of various pathogenic bacteria, the survival rate of artificially cultivated crickets has reached more than 90%.

——基因品系高,领地意识强烈。通过有意识地定向培养,蟋蟀种群品系稳定,血统纯正,多孔扣板的空间环境使其生长过程中达到一虫一窝,领地占有意识强烈,成虫持续打斗能力强。——High genetic strain, strong sense of territory. Through conscious directional cultivation, the cricket population has stable strains and pure bloodlines. The space environment of the porous gussets makes it possible to have one worm and one nest during the growth process. The crickets have a strong sense of territorial possession and strong continuous fighting ability of adults.

实施例2Example 2

无土温控人工培养蟋蟀的方法,包括以下步骤:The method for artificially cultivating crickets without soil temperature control comprises the following steps:

1.野生蟋蟀受精籽的冻籽处理1. Frozen seed treatment of wild cricket fertilized seeds

(1)野生蟋蟀受精籽的获得:将野生蟋蟀育种专用的细沙预先采用80目的筛子细筛成干沙土颗粒,在野生母本扎籽后,放置6-7天左右,使蟋蟀籽发育膨胀,发育状态连续观察如下:(1) Obtaining the fertilized seeds of wild crickets: the fine sand specially used for wild cricket breeding is sieved into dry sand particles with an 80-mesh sieve in advance. , the developmental state is continuously observed as follows:

1-3天籽呈现半透明状,开始萌发;1-3 days, the seeds are translucent and begin to germinate;

5-10天开始颜色变白,体积膨胀;After 5-10 days, the color turns white and the volume expands;

20天左右每颗籽体积大于80目。In about 20 days, the volume of each seed is greater than 80 mesh.

将采集的野生蟋蟀受精籽与细沙的混合标样以80目的筛子细筛,得到沙土分离的野生蟋蟀受精籽,用火焰灭菌的镊子和解剖针挑入无菌纸袋放置。The collected wild cricket fertilized seeds and fine sand mixed standard samples were finely sieved with an 80-mesh sieve to obtain wild cricket fertilized seeds separated from sand and soil, and were picked into sterile paper bags with flame-sterilized forceps and dissecting needles.

(2)野生蟋蟀受精籽的的灭菌处理(2) Sterilization of fertilized seeds of wild crickets

将野生蟋蟀受精籽的以质量百分比为5‰的高锰酸钾水溶液进行杀菌处理,由于野生蟋蟀受精籽的表面有不溶于水的蜡质层,使用木质牙签搅拌使其下沉入水,以充分消毒杀菌处理。The fertilized seeds of wild crickets are sterilized with a potassium permanganate aqueous solution of 5 ‰ by mass percentage. Since the fertilized seeds of wild crickets have a water-insoluble waxy layer, use a wooden toothpick to stir and make them sink into the water to Fully disinfect and sterilize.

(3)野生蟋蟀受精籽的冻籽处理(3) Freezing seed treatment of wild cricket fertilized seeds

将100℃以上火烤、自然冷却后的干沙土与灭菌处理后的野生蟋蟀受精籽混合,保持混合物总体湿度低于5%、捏手不能成团的状态,并将干沙土野生蟋蟀受精籽的混合物放入保鲜袋密封,置于2-10℃的低温环境中100天以上,充分模拟野生籽过冬环境;可根据孵化时间确定保存温度,计划100天-150天就要孵化的籽温度控制在100℃,计划1年以上才要孵化的籽温度控制在2℃左右;这期间自然死亡的籽约为5%左右,呈现明显的蜡白干瘪状。Mix the dry sand that has been baked at a temperature above 100°C and naturally cooled with the sterilized wild cricket fertilized seeds, keep the overall humidity of the mixture below 5%, and keep the mixture in a state that cannot form agglomerates when kneading hands, and dry the fertilized wild cricket seeds on the dry sand soil Put the mixture into a fresh-keeping bag and seal it, and place it in a low temperature environment of 2-10°C for more than 100 days, fully simulating the winter environment of wild seeds; the storage temperature can be determined according to the incubation time, and the temperature of the seeds that will be hatched in 100-150 days is planned to be controlled At 100°C, the temperature of the seeds that are planned to hatch for more than one year is controlled at about 2°C; during this period, about 5% of the seeds died naturally, showing an obvious waxy white and shriveled shape.

2.无土恒温孵化2. Soilless constant temperature incubation

(1)培养环境的准备(1) Preparation of the culture environment

人工培养房采用10cm厚的夹心泡沫板贴墙设置作为保温房,排列放置孵化架,架上统一铺置长40cm、宽30cm、高30cm的泡沫保温箱作为无土培育蟋蟀的培养缸,缸口敞开,上壁贴上5cm宽的光滑胶带,防止蟋蟀若虫外逃。The artificial culture room adopts 10cm thick sandwich foam boards to stick to the wall and set it as the heat preservation room. The hatching racks are arranged in a row, and the foam incubators with a length of 40 cm, a width of 30 cm and a height of 30 cm are uniformly laid on the racks as a cultivation tank for cultivating crickets without soil. Open it, and paste a 5cm wide smooth tape on the upper wall to prevent the cricket nymph from escaping.

培养缸的恒温恒湿无菌处理。蟋蟀致病源主要为螨虫、寄生蜂、多角体病毒和带病基因遗传。将培养缸预先使用电热暖风加热器组合带插座的温度控制器进行50-60℃高温杀菌、同时使用加湿器组合带插座的湿度控制器进行10%以内的低湿度杀菌,采用该方法持续处理10小时能够有效杀灭蟋蟀致病细菌和病毒。Constant temperature and humidity aseptic treatment of the culture tank. The pathogenic sources of crickets are mainly mites, parasitic wasps, polyhedrosis virus and disease-carrying genetic inheritance. Use the electric warm air heater combined with a temperature controller with a socket to carry out high-temperature sterilization at 50-60°C in advance, and at the same time use a humidifier combined with a humidity controller with a socket to carry out low-humidity sterilization within 10%. This method is used for continuous treatment 10 hours can effectively kill cricket pathogenic bacteria and viruses.

(2)野生蟋蟀受精籽的恒温培养(2) Constant temperature cultivation of fertilized seeds of wild crickets

经冻籽处理后的野生蟋蟀受精籽和干沙土混合物在室内自然温度下放置1天,喷洒水雾使湿度达到60%左右,搅拌后捏手可成团,搅拌后可散开,将铺在不锈钢的孵化盘(尺寸为20*30)上,铺设厚度在2-3cm,置于培养缸中恒温保湿培养6天,控制室温26℃,湿度90%。The fertilized seeds of wild crickets treated with frozen seeds and the mixture of dry sand and soil are placed at the natural temperature of the room for 1 day, sprayed with water mist to make the humidity reach about 60%, and can be kneaded into agglomerates after stirring, and can be dispersed after stirring. On the stainless steel incubator tray (size 20*30), the laying thickness is 2-3cm, placed in the culture tank for constant temperature and moisture cultivation for 6 days, the room temperature is controlled at 26°C, and the humidity is 90%.

为了提高蟋蟀出籽率,混合蟋蟀籽的细沙铺设厚度不能高于3cm,以保证所有籽的均匀受热,这样出籽率在90%以上,而且能够6-7天以内同步孵化。In order to improve the seed yield of crickets, the thickness of the fine sand mixed with cricket seeds should not be higher than 3cm to ensure that all seeds are evenly heated, so that the seed yield rate is above 90%, and they can be hatched simultaneously within 6-7 days.

其中,需要注意的事项:Among them, matters needing attention:

——期间若喷洒冷水雾,将导致60%以上孵化的热籽受凉死亡。——If cold water mist is sprayed during this period, more than 60% of the hatched hot seeds will die from the cold.

——孵化期内不可失水(籽会干瘪),也不可湿度过高(籽会窒息而死并发霉),否则蟋蟀籽将全部死亡。- During the incubation period there must be no loss of water (seeds will dry out) nor excessive humidity (seeds will suffocate and mold), otherwise all cricket seeds will die.

——孵化期内不可受到光线直射,否则籽会发红死亡。——Do not expose to direct light during the hatching period, otherwise the seeds will turn red and die.

——混合蟋蟀籽的细沙铺设厚度高于3cm,将导致过厚的蟋蟀籽上热下冷,上层籽提前孵化,而下层籽延后孵化,孵化时间相差过大,导致后孵化的小龄若虫被大龄若虫吃掉。——The thickness of the fine sand mixed with cricket seeds is more than 3cm, which will cause the top layer of cricket seeds to be hot and the bottom layer to be cold. The upper layer of seeds will hatch earlier, while the lower layer of seeds will hatch later. Nymphs are eaten by older nymphs.

3.蟋蟀1-4蜕若虫的变温培养3. Variable temperature culture of cricket 1-4 molting nymphs

以内径3*3毫米的多孔PVC扣板条(6cm*20cm)斜搭桥砌作为各龄蟋蟀若虫的活动场地,在保温箱内堆叠2-3层。贴合蟋蟀昼伏夜出的生物特性,白天采用低室温26℃、低湿度30%,有利于蟋蟀若虫休眠;夜晚采用高室温32℃、高湿度90%,促进蟋蟀若虫夜晚进食和活动发育。饲料以质量百分比为35:40:35:5的对虾养殖饲料、玉米、豆皮和蝉蜕,搅拌成颗粒状干粉,洒在缸内,内含野生环境下蟋蟀能够从土壤环境中所摄取的蛋白质、钙、电解多维等微量元素。同时将白菜叶切成1mm*1mm的碎末,撒在表层,供若虫食用补充自然养分。饮水为长10cm,宽20cm的浅水盆上盖湿布,可以有效避免蟋蟀若虫落水致死。The porous PVC gusset strips (6cm*20cm) with an inner diameter of 3*3mm are used as the activity site for cricket nymphs of all ages, and 2-3 layers are stacked in the incubator. In line with the nocturnal biological characteristics of crickets, a low room temperature of 26°C and a low humidity of 30% during the day is conducive to the dormancy of cricket nymphs; a high room temperature of 32°C and a high humidity of 90% at night promotes nighttime feeding and activity development of cricket nymphs. The feed is 35:40:35:5 mass percentage of prawn breeding feed, corn, bean skin and cicada slough, stirred into granular dry powder, sprinkled in the tank, which contains the protein that crickets can ingest from the soil environment in the wild environment , calcium, electrolytic multidimensional and other trace elements. At the same time, cut the cabbage leaves into 1mm*1mm pieces, sprinkle them on the surface, and feed the nymphs to supplement natural nutrients. Drinking water is a shallow water basin with a length of 10 cm and a width of 20 cm, covered with a wet cloth, which can effectively prevent the death of cricket nymphs from falling into the water.

4.蟋蟀5-6蜕大龄若虫的分缸培养4. Cricket culture of 5-6 molting nymphs in separate tanks

生长40天以后,若虫达到5蜕左右,开始分缸,平均一缸分成10缸。分缸过程中,小孔板换成内径1*1cm的多孔PVC扣板条(6cm*20cm)纵横搭桥砖砌作为各龄蟋蟀若虫的活动场地,在保温箱内堆叠2-3层。另外以加热调成糊状的湿饲料抹在白菜叶上作为辅助饲料,给蜕皮后不久的嫩皮蟋蟀若虫食用,其余环境参数与步骤3一致。After 40 days of growth, the nymphs reach about 5 sloughs and start to divide into tanks, with an average of 10 tanks per tank. During the tank division process, the small orifice plate was replaced with porous PVC gusset strips (6cm*20cm) with an inner diameter of 1*1cm, and the vertical and horizontal bridging bricks were used as the activity site for cricket nymphs of all ages, and 2-3 layers were stacked in the incubator. In addition, the wet feed adjusted to paste by heating is used as auxiliary feed on the cabbage leaves, and it is eaten by the tender skin cricket nymphs shortly after molting, and the rest of the environmental parameters are consistent with step 3.

培养结果显示,通过无土温度控制方法人工培养的蟋蟀,具有以下特点:The cultivation results show that the crickets artificially cultivated by the soilless temperature control method have the following characteristics:

——成虫个头大,皮色好。经过人工优化选育和分缸培养,蟋蟀普遍个体成长度高,平均重量在60毫克以上。——The adults are big and have good skin color. After artificially optimized breeding and sub-tank cultivation, crickets generally have a high individual growth rate, with an average weight of more than 60 mg.

——成活率高。由于人工培养解决了蟋蟀自然天敌的侵害,无土环境又极大地降低了各类致病菌的侵害,使得人工培养的蟋蟀成活率达到了90%以上。——High survival rate. Because the artificial cultivation solves the invasion of crickets' natural enemies, and the soilless environment greatly reduces the invasion of various pathogenic bacteria, the survival rate of artificially cultivated crickets has reached more than 90%.

——基因品系高,领地意识强烈。通过有意识地定向培养,蟋蟀种群品系稳定,血统纯正,多孔扣板的空间环境使其生长过程中达到一虫一窝,领地占有意识强烈,成虫持续打斗能力强。——High genetic strain, strong sense of territory. Through conscious directional cultivation, the cricket population has stable strains and pure bloodlines. The space environment of the porous gussets makes it possible to have one worm and one nest during the growth process. The crickets have a strong sense of territorial possession and strong continuous fighting ability of adults.

实施例3Example 3

无土温控人工培养蟋蟀的方法,包括以下步骤:The method for artificially cultivating crickets without soil temperature control comprises the following steps:

1.野生蟋蟀受精籽的冻籽处理1. Frozen seed treatment of wild cricket fertilized seeds

(1)野生蟋蟀受精籽的获得:将野生蟋蟀育种专用的细沙预先采用80目的筛子细筛成干沙土颗粒,在野生母本扎籽后,放置6-7天左右,使蟋蟀籽发育膨胀,发育状态连续观察如下:(1) Obtaining the fertilized seeds of wild crickets: the fine sand specially used for wild cricket breeding is sieved into dry sand particles with an 80-mesh sieve in advance. , the developmental state is continuously observed as follows:

1-3天籽呈现半透明状,开始萌发;1-3 days, the seeds are translucent and begin to germinate;

5-10天开始颜色变白,体积膨胀;After 5-10 days, the color turns white and the volume expands;

20天左右每颗籽体积大于80目。In about 20 days, the volume of each seed is greater than 80 mesh.

将采集的野生蟋蟀受精籽与细沙的混合标样以80目的筛子细筛,得到沙土分离的野生蟋蟀受精籽,用火焰灭菌的镊子和解剖针挑入无菌纸袋放置。The collected wild cricket fertilized seeds and fine sand mixed standard samples were finely sieved with an 80-mesh sieve to obtain wild cricket fertilized seeds separated from sand and soil, and were picked into sterile paper bags with flame-sterilized forceps and dissecting needles.

(2)野生蟋蟀受精籽的的灭菌处理(2) Sterilization of fertilized seeds of wild crickets

将野生蟋蟀受精籽的以质量百分比为6‰的高锰酸钾水溶液进行杀菌处理,由于野生蟋蟀受精籽的表面有不溶于水的蜡质层,使用木质牙签搅拌使其下沉入水,以充分消毒杀菌处理。The fertilized seeds of wild crickets are sterilized with a potassium permanganate aqueous solution of 6‰ in mass percent, because the surface of the fertilized seeds of wild crickets has a water-insoluble waxy layer, stir it with a wooden toothpick to sink into the water, and Fully disinfect and sterilize.

(3)野生蟋蟀受精籽的冻籽处理(3) Freezing seed treatment of wild cricket fertilized seeds

将100℃以上火烤、自然冷却后的干沙土与灭菌处理后的野生蟋蟀受精籽混合,保持混合物总体湿度低于5%、捏手不能成团的状态,并将干沙土野生蟋蟀受精籽的混合物放入保鲜袋密封,置于2-10℃的低温环境中100天以上,充分模拟野生籽过冬环境;可根据孵化时间确定保存温度,计划100天-150天就要孵化的籽温度控制在100℃,计划1年以上才要孵化的籽温度控制在2℃左右;这期间自然死亡的籽约为5%左右,呈现明显的蜡白干瘪状。Mix the dry sand that has been baked at a temperature above 100°C and naturally cooled with the sterilized wild cricket fertilized seeds, keep the overall humidity of the mixture below 5%, and keep the mixture in a state that cannot form agglomerates when kneading hands, and dry the fertilized wild cricket seeds on the dry sand soil Put the mixture into a fresh-keeping bag and seal it, and place it in a low temperature environment of 2-10°C for more than 100 days, fully simulating the winter environment of wild seeds; the storage temperature can be determined according to the incubation time, and the temperature of the seeds that will be hatched in 100-150 days is planned to be controlled At 100°C, the temperature of the seeds that are planned to hatch for more than one year is controlled at about 2°C; during this period, about 5% of the seeds died naturally, showing an obvious waxy white and shriveled shape.

2.无土恒温孵化2. Soilless constant temperature incubation

(1)培养环境的准备(1) Preparation of the culture environment

人工培养房采用10cm厚的夹心泡沫板贴墙设置作为保温房,排列放置孵化架,架上统一铺置长40cm、宽30cm、高30cm的泡沫保温箱作为无土培育蟋蟀的培养缸,缸口敞开,上壁贴上5cm宽的光滑胶带,防止蟋蟀若虫外逃。The artificial culture room adopts 10cm thick sandwich foam boards to stick to the wall and set it as the heat preservation room. The hatching racks are arranged in a row, and the foam incubators with a length of 40 cm, a width of 30 cm and a height of 30 cm are uniformly laid on the racks as a cultivation tank for cultivating crickets without soil. Open it, and paste a 5cm wide smooth tape on the upper wall to prevent the cricket nymph from escaping.

培养缸的恒温恒湿无菌处理。蟋蟀致病源主要为螨虫、寄生蜂、多角体病毒和带病基因遗传。将培养缸预先使用电热暖风加热器组合带插座的温度控制器进行50-60℃高温杀菌、同时使用加湿器组合带插座的湿度控制器进行10%以内的低湿度杀菌,采用该方法持续处理10小时能够有效杀灭蟋蟀致病细菌和病毒。Constant temperature and humidity aseptic treatment of the culture tank. The pathogenic sources of crickets are mainly mites, parasitic wasps, polyhedrosis virus and disease-carrying genetic inheritance. Use the electric warm air heater combined with a temperature controller with a socket to carry out high-temperature sterilization at 50-60°C in advance, and at the same time use a humidifier combined with a humidity controller with a socket to carry out low-humidity sterilization within 10%. This method is used for continuous treatment 10 hours can effectively kill cricket pathogenic bacteria and viruses.

(2)野生蟋蟀受精籽的恒温培养(2) Constant temperature cultivation of fertilized seeds of wild crickets

经冻籽处理后的野生蟋蟀受精籽和干沙土混合物在室内自然温度下放置2天,喷洒水雾使湿度达到60%左右,搅拌后捏手可成团,搅拌后可散开,将铺在不锈钢的孵化盘(尺寸为20*30)上,铺设厚度在2-3cm,置于培养缸中恒温保湿培养7天,控制室温30℃,湿度80%。The fertilized seeds of wild crickets treated with frozen seeds and the mixture of dry sand and soil are placed at the natural temperature of the room for 2 days, sprayed with water mist to make the humidity reach about 60%. On the stainless steel incubator tray (size 20*30), the laying thickness is 2-3cm, placed in the culture tank for 7 days at constant temperature and moisture, and the room temperature is controlled at 30°C and the humidity is 80%.

为了提高蟋蟀出籽率,混合蟋蟀籽的细沙铺设厚度不能高于3cm,以保证所有籽的均匀受热,这样出籽率在90%以上,而且能够6-7天以内同步孵化。In order to improve the seed yield of crickets, the thickness of the fine sand mixed with cricket seeds should not be higher than 3cm to ensure that all seeds are evenly heated, so that the seed yield rate is above 90%, and they can be hatched simultaneously within 6-7 days.

其中,需要注意的事项:Among them, matters needing attention:

——期间若喷洒冷水雾,将导致60%以上孵化的热籽受凉死亡。——If cold water mist is sprayed during this period, more than 60% of the hatched hot seeds will die from the cold.

——孵化期内不可失水(籽会干瘪),也不可湿度过高(籽会窒息而死并发霉),否则蟋蟀籽将全部死亡。- During the incubation period there must be no loss of water (seeds will dry out) nor excessive humidity (seeds will suffocate and mold), otherwise all cricket seeds will die.

——孵化期内不可受到光线直射,否则籽会发红死亡。——Do not expose to direct light during the hatching period, otherwise the seeds will turn red and die.

——混合蟋蟀籽的细沙铺设厚度高于3cm,将导致过厚的蟋蟀籽上热下冷,上层籽提前孵化,而下层籽延后孵化,孵化时间相差过大,导致后孵化的小龄若虫被大龄若虫吃掉。——The thickness of the fine sand mixed with cricket seeds is more than 3cm, which will cause the top layer of cricket seeds to be hot and the bottom layer to be cold. The upper layer of seeds will hatch earlier, while the lower layer of seeds will hatch later. Nymphs are eaten by older nymphs.

3.蟋蟀1-4蜕若虫的变温培养3. Variable temperature culture of cricket 1-4 molting nymphs

以内径3*3毫米的多孔PVC扣板条(6cm*20cm)斜搭桥砌作为各龄蟋蟀若虫的活动场地,在保温箱内堆叠2-3层。贴合蟋蟀昼伏夜出的生物特性,白天采用低室温28℃、低湿度50%,有利于蟋蟀若虫休眠;夜晚采用高室温31℃、高湿度80%,促进蟋蟀若虫夜晚进食和活动发育。饲料以质量百分比为25:30:25:5的对虾养殖饲料、玉米、豆皮和蝉蜕,搅拌成颗粒状干粉,洒在缸内,内含野生环境下蟋蟀能够从土壤环境中所摄取的蛋白质、钙、电解多维等微量元素。同时将白菜叶切成1mm*1mm的碎末,撒在表层,供若虫食用补充自然养分。饮水为长10cm,宽20cm的浅水盆上盖湿布,可以有效避免蟋蟀若虫落水致死。The porous PVC gusset strips (6cm*20cm) with an inner diameter of 3*3mm are used as the activity site for cricket nymphs of all ages, and 2-3 layers are stacked in the incubator. In line with the biological characteristics of nocturnal crickets, a low room temperature of 28°C and a low humidity of 50% during the day is conducive to the dormancy of cricket nymphs; a high room temperature of 31°C and a high humidity of 80% at night promotes nighttime feeding and activity development of cricket nymphs. The feed is 25:30:25:5 mass percentage of prawn breeding feed, corn, bean skin and cicada slough, stirred into granular dry powder, sprinkled in the tank, which contains the protein that crickets can ingest from the soil environment in the wild environment , calcium, electrolytic multidimensional and other trace elements. At the same time, cut the cabbage leaves into 1mm*1mm pieces, sprinkle them on the surface, and feed the nymphs to supplement natural nutrients. Drinking water is a shallow water basin with a length of 10 cm and a width of 20 cm, covered with a wet cloth, which can effectively prevent the death of cricket nymphs from falling into the water.

4.蟋蟀5-6蜕大龄若虫的分缸培养4. Cricket culture of 5-6 molting nymphs in separate tanks

生长40天以后,若虫达到5蜕左右,开始分缸,平均一缸分成10缸。分缸过程中,小孔板换成内径1*1cm的多孔PVC扣板条(6cm*20cm)纵横搭桥砖砌作为各龄蟋蟀若虫的活动场地,在保温箱内堆叠2-3层。另外以加热调成糊状的湿饲料抹在白菜叶上作为辅助饲料,给蜕皮后不久的嫩皮蟋蟀若虫食用,其余环境参数与步骤3一致。After 40 days of growth, the nymphs reach about 5 sloughs and start to divide into tanks, with an average of 10 tanks per tank. During the tank division process, the small orifice plate was replaced with porous PVC gusset strips (6cm*20cm) with an inner diameter of 1*1cm, and the vertical and horizontal bridging bricks were used as the activity site for cricket nymphs of all ages, and 2-3 layers were stacked in the incubator. In addition, the wet feed adjusted to paste by heating is used as auxiliary feed on the cabbage leaves, and it is eaten by the tender skin cricket nymphs shortly after molting, and the rest of the environmental parameters are consistent with step 3.

培养结果显示,通过无土温度控制方法人工培养的蟋蟀,具有以下特点:The cultivation results show that the crickets artificially cultivated by the soilless temperature control method have the following characteristics:

——成虫个头大,皮色好。经过人工优化选育和分缸培养,蟋蟀普遍个体成长度高,平均重量在60毫克以上。——The adults are big and have good skin color. After artificially optimized breeding and sub-tank cultivation, crickets generally have a high individual growth rate, with an average weight of more than 60 mg.

——成活率高。由于人工培养解决了蟋蟀自然天敌的侵害,无土环境又极大地降低了各类致病菌的侵害,使得人工培养的蟋蟀成活率达到了90%以上。——High survival rate. Because the artificial cultivation solves the invasion of crickets' natural enemies, and the soilless environment greatly reduces the invasion of various pathogenic bacteria, the survival rate of artificially cultivated crickets has reached more than 90%.

——基因品系高,领地意识强烈。通过有意识地定向培养,蟋蟀种群品系稳定,血统纯正,多孔扣板的空间环境使其生长过程中达到一虫一窝,领地占有意识强烈,成虫持续打斗能力强。——High genetic strain, strong sense of territory. Through conscious directional cultivation, the cricket population has stable strains and pure bloodlines. The space environment of the porous gussets makes it possible to have one worm and one nest during the growth process. The crickets have a strong sense of territorial possession and strong continuous fighting ability of adults.

Claims (5)

1. without soil temperature control, manually cultivate a method for cricket, it is characterized in that: comprise the following steps:
(1) seed that freezes of wild cricket fertilization seed is processed: wild cricket fertilization seed and dry sandy soil mix to be placed in temperature 2-10 ℃ to be processed more than 100 days through freezing seed;
(2) constant temperature culture of wild cricket fertilization seed: the wild cricket fertilization seed after freezing seed processing was cultivated after 1-2 days under natural temperature, and in the aquarium after sterilizing, constant-temperature moisture-keeping is cultivated 6-7 days, and condition of culture is: temperature 25-30 ℃, humidity 80-90%;
(3) cricket 1-4 is sloughed off the Fluctuation temperature culture of nymph: the porous PVC buckle bar oblique bridge of usining is built the space for activities as cricket nymph in each in age, stacking layer 2-3 in incubator, wild cricket after constant temperature culture is fertilized to seed in day and night cultivating 40-45 days in varying temperature environment, condition of culture is: day temperature is that 25-28 ℃, humidity are 30-50%, nocturnal temperature is that 30-32 ℃, humidity are 80-90%, and feed is mixture and the Chinese cabbage leaf of prawn culturing feed, corn, skin of beancurd and cicada slough;
(4) cricket 5-6 is sloughed off minute cylinder cultivation of Elder nymph: nymph reaches 5 and sloughs off cylinder cultivation in rear minute, average 1 cylinder is divided into 10 cylinders, condition of culture is: day temperature is that 25-28 ℃, humidity are 30-50%, nocturnal temperature is that 30-32 ℃, humidity are 80-90%, and feed is mixture and the Chinese cabbage leaf of prawn culturing feed, corn, skin of beancurd and cicada slough.
2. a kind of method of manually cultivating cricket without soil temperature control according to claim 1, is characterized in that: in step (1), the preservation of wild cricket fertilization seed adopts aseptic paper bag.
3. a kind of method of manually cultivating cricket without soil temperature control according to claim 1, is characterized in that: in step (1), described wild cricket fertilization seed is the fertilization seed through mass percent 5-6 ‰ potassium permanganate solution sterilization processing.
4. a kind of method of manually cultivating cricket without soil temperature control according to claim 1, is characterized in that: in step (2), the sterilizing methods of aquarium is that 50-60 ℃, humidity are that environment below 10% is processed 8-10h for aquarium being placed in to temperature.
5. a kind of method of manually cultivating cricket without soil temperature control according to claim 1, is characterized in that: in step (3), in feed, the mass ratio of prawn culturing feed, corn, skin of beancurd and cicada slough is (25-35): (30-40): (25-35): 5.
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CN104115792A (en) * 2014-08-08 2014-10-29 太仓市永发农场专业合作社 Long-horned grasshopper breeding method
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CN111165438A (en) * 2020-03-24 2020-05-19 山东万广农业科技有限公司 A method for off-season hatching of black grasshopper eggs

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2590371Y (en) * 2002-11-13 2003-12-10 纪家祥 Cricket thermostat rearing box
CN201029375Y (en) * 2007-01-26 2008-03-05 顾云冬 Automatic temperature control cricket basin

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2829852B2 (en) * 1996-12-30 1998-12-02 学 立石 Breeding crickets for feed

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2590371Y (en) * 2002-11-13 2003-12-10 纪家祥 Cricket thermostat rearing box
CN201029375Y (en) * 2007-01-26 2008-03-05 顾云冬 Automatic temperature control cricket basin

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
JP特开平10-191834A 1998.07.28
人工养殖蟋蟀;韩素芹;《当代畜禽养殖业》;20040815(第8期);第35页 *
刘敬泽等.实验室内蟋蟀的长期饲养和诱捕方法.《生物学通报》.1999,第34卷(第2期),第39页.
双斑蟋的室内饲养;李娜;《特种经济动植物》;20030228(第2期);第12页 *
实验室内蟋蟀的长期饲养与管理;李娜;《生物学通报》;20030420;第38卷(第4期);第59页 *
实验室内蟋蟀的长期饲养和诱捕方法;刘敬泽等;《生物学通报》;19990220;第34卷(第2期);第39页 *
李娜.双斑蟋的室内饲养.《特种经济动植物》.2003,(第2期),第12页.
李娜.实验室内蟋蟀的长期饲养与管理.《生物学通报》.2003,第38卷(第4期),第59页.
韩素芹.人工养殖蟋蟀.《当代畜禽养殖业》.2004,(第8期),第35页.

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