CN103013970A - Immobilized probiotics both rich in plant fiber and probiotics and preparation method thereof - Google Patents
Immobilized probiotics both rich in plant fiber and probiotics and preparation method thereof Download PDFInfo
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- CN103013970A CN103013970A CN2012105010710A CN201210501071A CN103013970A CN 103013970 A CN103013970 A CN 103013970A CN 2012105010710 A CN2012105010710 A CN 2012105010710A CN 201210501071 A CN201210501071 A CN 201210501071A CN 103013970 A CN103013970 A CN 103013970A
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- vegetable fibre
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- 239000006041 probiotic Substances 0.000 title claims abstract description 76
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 76
- 239000000835 fiber Substances 0.000 title claims abstract description 36
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- 239000000463 material Substances 0.000 claims abstract description 22
- 239000003094 microcapsule Substances 0.000 claims abstract description 16
- 239000000843 powder Substances 0.000 claims abstract description 13
- 241000894006 Bacteria Species 0.000 claims description 75
- 230000000529 probiotic effect Effects 0.000 claims description 66
- 235000013339 cereals Nutrition 0.000 claims description 35
- 235000013311 vegetables Nutrition 0.000 claims description 32
- 239000000243 solution Substances 0.000 claims description 22
- 239000000203 mixture Substances 0.000 claims description 19
- 230000001580 bacterial effect Effects 0.000 claims description 16
- 239000012531 culture fluid Substances 0.000 claims description 16
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 14
- 230000000694 effects Effects 0.000 claims description 13
- 239000004519 grease Substances 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 244000068988 Glycine max Species 0.000 claims description 9
- 235000010469 Glycine max Nutrition 0.000 claims description 9
- 235000013312 flour Nutrition 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 8
- 235000007164 Oryza sativa Nutrition 0.000 claims description 7
- 235000009566 rice Nutrition 0.000 claims description 7
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 7
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 7
- 238000005119 centrifugation Methods 0.000 claims description 6
- 102000004169 proteins and genes Human genes 0.000 claims description 6
- 108090000623 proteins and genes Proteins 0.000 claims description 6
- 239000007787 solid Substances 0.000 claims description 6
- 241000209140 Triticum Species 0.000 claims description 5
- 235000021307 Triticum Nutrition 0.000 claims description 5
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 5
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 5
- 235000019797 dipotassium phosphate Nutrition 0.000 claims description 5
- 239000008267 milk Substances 0.000 claims description 5
- 210000004080 milk Anatomy 0.000 claims description 5
- 235000013336 milk Nutrition 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 230000001105 regulatory effect Effects 0.000 claims description 4
- 239000007864 aqueous solution Substances 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- 239000010419 fine particle Substances 0.000 claims description 2
- 239000007858 starting material Substances 0.000 claims description 2
- 235000019156 vitamin B Nutrition 0.000 claims description 2
- 239000011720 vitamin B Substances 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims 2
- 239000002994 raw material Substances 0.000 abstract description 5
- 230000001502 supplementing effect Effects 0.000 abstract 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 10
- 235000015097 nutrients Nutrition 0.000 description 9
- 239000000126 substance Substances 0.000 description 7
- 241000209094 Oryza Species 0.000 description 5
- 235000013305 food Nutrition 0.000 description 5
- 235000014655 lactic acid Nutrition 0.000 description 5
- 230000002950 deficient Effects 0.000 description 4
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 3
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
- 229930003471 Vitamin B2 Natural products 0.000 description 3
- 230000000968 intestinal effect Effects 0.000 description 3
- 229960002477 riboflavin Drugs 0.000 description 3
- 239000001488 sodium phosphate Substances 0.000 description 3
- 229910000162 sodium phosphate Inorganic materials 0.000 description 3
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 3
- 238000001291 vacuum drying Methods 0.000 description 3
- 239000011716 vitamin B2 Substances 0.000 description 3
- 235000019164 vitamin B2 Nutrition 0.000 description 3
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 230000007413 intestinal health Effects 0.000 description 2
- 239000004310 lactic acid Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 206010010774 Constipation Diseases 0.000 description 1
- 208000005016 Intestinal Neoplasms Diseases 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000013872 defecation Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 229910010272 inorganic material Inorganic materials 0.000 description 1
- 239000011147 inorganic material Substances 0.000 description 1
- 201000002313 intestinal cancer Diseases 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000008855 peristalsis Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 229960001866 silicon dioxide Drugs 0.000 description 1
- 235000012239 silicon dioxide Nutrition 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000002910 solid waste Substances 0.000 description 1
- 239000004408 titanium dioxide Substances 0.000 description 1
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- Coloring Foods And Improving Nutritive Qualities (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention relates to immobilized probiotics both rich in plant fibers and probiotics and a preparation method thereof. The immobilized probiotics are microcapsule powder obtained by embedding the probiotics by taking the pure grain powder of the plant fibers as embedding materials. The raw materials of the immobilized probiotics comprise the following components in percentage by weight: 70%-80% of pure grain seeds and 20%-30% of thallus culture solutions. Compared with the prior art, the immobilized probiotics disclosed by the invention has the advantages of both supplementing the plant fiber and the probiotics, and the like.
Description
Technical field
The present invention relates to a kind of immobilization probiotic bacterium, especially relate to a kind of immobilization probiotic bacterium of being rich in simultaneously vegetable fibre and probiotic bacterium and preparation method thereof.
Background technology
Probiotic bacterium (Probiotics) is a kind of beneficial microorganism that can improve, nurse one's health human intestinal microbial balance, and its physiologic function obtains whole world scientific and technological circle and generally admits and be widely used in medicine, health care and food aspect.Vegetable fibre is a kind of carbohydrate that can not be digested by human body, and it has very important effect to promoting good digestion and draining solid waste.Vegetable fibre can make the food in the enteron aisle increase deliquescing, promotes intestines peristalsis, thereby accelerates defecation speed, prevents constipation and reduces the intestinal cancer risk.It is the indispensable the seventh-largest nutrient substance of human body.Grain flour embedded material take vegetable fibre as main nutrient elements is made the immobilization probiotic bacterium can make vegetable fibre and probiotic bacterium act on simultaneously human intestinal, and the dual function of two kinds of nutritive elements more is conducive to balance, improves human intestinal health.
Because at present probiotic bacterium adopts usually that the inorganic materials such as silicon-dioxide, activated alumina, titanium dioxide, diatomite or its are material modified to be carrier; And the natural organic high-molecular solid support material is as embedded material.The major defect of these embedded materials has: 1. these artificial polymkeric substance that are used for food have many uncertain factors, and existing natural organic high-molecular solid support material has physical strength lower, poor flexibility, under anaerobic easily decomposed by microorganism, can cause the defectives such as variation of mechanisms for drug release; 2. these artificial polymkeric substance can restrict the use range of live body probiotic bacterium greatly; 3. the immobilization probiotic bacterium of making take artificial polymkeric substance as embedded material can't be satisfied the demand of healthy enteron aisle because it contains a kind of nutritive element of profitable probliotics comprehensively.
Because the defective of above manufacture craft can't satisfy the existing market demand.And the grain flour embedded material take vegetable fibre as main nutrient elements makes the method for immobilization probiotic bacterium and not only overcome the defective that prior art exists, and also changed to use artificial polymkeric substance to be the form of probiotic bacterium carrier at present.The food raw material that vegetable fibre is rich in utilization is the embedding equivalent material, and this material not only can directly be eaten; Abundant vegetable fibre can improve and protect intestinal environment, and this grain flour embedded material that is rich in vegetable fibre can provide enough nutrients for probiotic bacterium simultaneously, strengthens its activity after entering enteron aisle; Intestinal health can effectively be takeed good care of, be improved, protect to the double effects of probiotic bacterium and vegetable fibre.
Summary of the invention
Purpose of the present invention is exactly to provide a kind of immobilization probiotic bacterium of being rich in simultaneously vegetable fibre and probiotic bacterium and preparation method thereof for the defective that overcomes above-mentioned prior art existence.
Purpose of the present invention can be achieved through the following technical solutions: a kind of immobilization probiotic bacterium of being rich in simultaneously vegetable fibre and probiotic bacterium, it is characterized in that, the microscapsule powder of this immobilization probiotic bacterium for obtaining as embedded material embedding probiotic bacterium take the pure grain flour that is rich in vegetable fibre, its starting material comprise the component of prescription:
Pure cereal seed: 70～80wt%;
Bacterial culture fluid: 20～30wt%.
Described pure cereal seed comprises one or more in soybean, wheat, the rice.
Described pure cereal seed is mixed with mass ratio by soybean, wheat, rice at 7: 2: 1.
Described bacterial culture fluid is to add the milk powder 1.5wt% that contains probiotic bacterium, potassium hydrogen phosphate 0.06wt%, vitamins B in water
20.01wt%, the rear sterilization that stirs makes.
Described bacterial culture fluid is the aqueous solution of commercially available probiotic bacterium, and mass percent concentration is 1-2%.
The particle diameter of described microcapsule is 1～5 μ m.
A kind of preparation method who is rich in simultaneously the immobilization probiotic bacterium of vegetable fibre and probiotic bacterium, it is characterized in that, the method may further comprise the steps: 8-12h at normal temperatures is soaked in water pure cereal seed, defibrination, centrifugation macrobead throw out again, the insoluble composition of getting soluble part and suspending carries out self-emulsifying to be processed, regulating the pH value is 7.2-7.5, surface of microcapsule is electronegative in the solution after self-emulsifying is processed, the cereal colloidal solution that obtains is become fine-particle powder, being fixed probiotic products with bacterial culture fluid mixing postlyophilization.
The cereal colloidal solution that described cereal colloidal solution is solid 40%, the viable count of described bacterial culture fluid＞1000/ gram.
The pH value that described self-emulsifying is treated to the insoluble composition of regulating described soluble part and suspending is 7-8, and in homogenizer, stir 10-30min, make that protein transfers to meta-alkalescence in the cereal, under the effect of high speed shear power, form albumen-grease microcapsule with the contained grease of cereal itself; Described cryodesiccated temperature is-40 ℃～0 ℃.
Described pH value adopts sodium bicarbonate to regulate.
Compared with prior art, the present invention has the following advantages:
1, the embedded material itself that is rich in vegetable fibre is the nutrient of probiotic bacterium, can guarantee the long period of activity of probiotic bacterium;
2, the combination of probiotic bacterium and vegetable fibre can make the effect maximization that improves enteron aisle;
3, products obtained therefrom is that immobilization material is direct-edible;
4, products obtained therefrom is that immobilization material itself is food, and is safe and reliable;
5, products obtained therefrom is the immobilization material convenient sources.
Description of drawings
Fig. 1 is the Electronic Speculum figure of immobilization probiotic products of the present invention.
Embodiment
The present invention is described in detail below in conjunction with the drawings and specific embodiments.
Embodiment 1 (lactic bacteria activity powder-product)
Grain flour embedded material take vegetable fibre as main nutrient elements is made the method for immobilization probiotic bacterium, and the method comprises following processing step:
1. the mixture that mixes at 7: 2: 1 in mass ratio of soybean wheat rice is made living lactic acid bacteria powder product as investment;
2. after getting at normal temperatures step (1) gained mixture 70kg and being soaked in water 8-12 hour, defibrination, centrifugation macrobead throw out composition again, the insoluble composition of getting soluble part and suspending carries out self-emulsifying to be processed, namely adopt sodium bicarbonate to regulate described soluble part and the pH value of the insoluble composition that can suspend is 7.2, and in homogenizer, stirred 15 minutes, make that protein transfers to meta-alkalescence in the cereal, under the effect of high speed shear power, form albumen-grease microcapsule with the contained grease of cereal itself, and to regulate pH be that surface of microcapsule is electronegative in 7.2 solution that make after self-emulsifying is processed, and produces solid substance and be 40% colloidal solution;
3. add lactic acid bacteria culture solution 30kg at resulting colloidal solution at last, at-40 ℃ of lower being fixed of vacuum-drying probiotic bacterium pulvis products, this product is microencapsulated after mixing, and the particle diameter of microcapsule is 1～5 μ m, and the Electronic Speculum figure of products obtained therefrom as shown in Figure 1.
Described bacterial culture fluid is: add the milk powder 1.5wt% that contains the lactic bacteria activity powder in water, potassium hydrogen phosphate 0.06wt% Lin Suanna Vitamin B2 Sodium Phosphate 0.01wt% stirs evenly rear sterilization and makes.
Embodiment 2 (the dried bacterium of two qis)
Grain flour embedded material take vegetable fibre as main nutrient elements is made the method for immobilization probiotic bacterium, and the method comprises following processing step:
1. the mixture that mixes at 7: 3 in mass ratio take soybean and rice is as raw material,
Get be soaked in water 8-12 hour under the described mixture 80kg of above-mentioned steps (1) normal temperature after, defibrination, centrifugation insoluble composition again, the insoluble composition of getting soluble part and suspending carries out self-emulsifying to be processed: the pH value that adopts sodium bicarbonate to regulate described soluble part is 7.5, and in homogenizer, stirred 20 minutes, make that protein transfers to meta-alkalescence in the cereal, under the effect of high speed shear power, form albumen-grease microcapsule with the contained grease of cereal itself;
3. adopting sodium bicarbonate to regulate pH is the electronegative colloidal solution that obtains of surface of microcapsule in 7.5 solution that make after self-emulsifying is processed;
4. the last bacterial culture fluid 20kg that adds in the colloidal solution that obtains mixes rear at the probiotic bacterium of being fixed of vacuum-drying below-30 ℃ pulvis.
Described bacterial culture fluid is for adding the milk powder 1.5wt% that contains two dried bacterium of qi in water, potassium hydrogen phosphate 0.06wt% Lin Suanna Vitamin B2 Sodium Phosphate 0.01wt% stirs evenly rear sterilization and makes.
Embodiment 3 (milk-acid bacteria+dried bacterium+yeast of two qis)
Grain flour embedded material take vegetable fibre as main nutrient elements is made the method for immobilization probiotic bacterium, and the method comprises following processing step:
1. the mixture that mixes at 8: 2 in mass ratio take soybean and rice is as raw material;
2. after going the described mixture 75kg of step (1) to be soaked in water at normal temperatures 8-12 hour, defibrination, again centrifugation insoluble composition;
3. the insoluble composition of getting soluble part and suspending carries out self-emulsifying to be processed, and regulates pH and make in the solution after self-emulsifying is processed surface of microcapsule electronegative;
4. add probiotic's culture liquid 25kg (the dried bacterium of milk-acid bacteria+two qis+saccharomycetes to make fermentation liquid makes viable bacteria finally reach 1,000 hundred million/gram) at the colloidal solution that obtains at last, solid substance is transferred to lyophilize below 40%, 0 ℃ make immobilization probiotic bacterium pulvis.
Described bacterial culture fluid contains milk-acid bacteria, the dried bacterium of two qi and saccharomycetic milk powder 1.5wt% for adding in water, potassium hydrogen phosphate 0.06wt% Lin Suanna Vitamin B2 Sodium Phosphate 0.01wt% stirs evenly rear sterilization and makes.
Described self-emulsifying is treated to protein in the cereal is transferred to meta-alkalescence, stirs under the effect of high speed shear power with the contained grease of cereal itself and forms albumen-grease microcapsule in 10 minutes.
Described adjusting pH adopts sodium bicarbonate, and it is 7.4 that adjusting pH makes the pH value of the solution after self-emulsifying is processed.
Embodiment 4
Grain flour embedded material take vegetable fibre as main nutrient elements is made the method for immobilization probiotic bacterium, and the method comprises following processing step:
Take soybean as raw material;
Get be soaked in water 8-12 hour under the described soybean 78kg of above-mentioned steps (1) normal temperature after, defibrination, centrifugation insoluble composition again, the insoluble composition of getting soluble part and suspending carries out self-emulsifying to be processed: the pH value that adopts sodium bicarbonate to regulate described soluble part is 7.5, and in homogenizer, stirred 20 minutes, make that protein transfers to meta-alkalescence in the cereal, under the effect of high speed shear power, form albumen-grease microcapsule with the contained grease of cereal itself;
7. adopting sodium bicarbonate to regulate pH is the electronegative colloidal solution that obtains of surface of microcapsule in 7.5 solution that make after self-emulsifying is processed;
8. the last bacterial culture fluid 22kg that adds in the colloidal solution that obtains mixes rear at the probiotic bacterium of being fixed of vacuum-drying below-30 ℃ pulvis.
Described bacterial culture fluid is the aqueous solution of commercially available probiotic bacterium, and the probiotic bacterium mass percent concentration is 1-2%.
Claims (10)
1. an immobilization probiotic bacterium of being rich in simultaneously vegetable fibre and probiotic bacterium is characterized in that, the microscapsule powder of this immobilization probiotic bacterium for obtaining as embedded material embedding probiotic bacterium take the pure grain flour that is rich in vegetable fibre, and its starting material comprise the component of prescription:
Pure cereal seed: 70～80wt%;
Bacterial culture fluid: 20～30wt%.
2. a kind of immobilization probiotic bacterium of being rich in simultaneously vegetable fibre and probiotic bacterium according to claim 1 is characterized in that, described pure cereal seed comprises one or more in soybean, wheat, the rice.
3. a kind of immobilization probiotic bacterium of being rich in simultaneously vegetable fibre and probiotic bacterium according to claim 1 and 2 is characterized in that, described pure cereal seed is mixed with mass ratio by soybean, wheat, rice at 7: 2: 1.
4. a kind of immobilization probiotic bacterium of being rich in simultaneously vegetable fibre and probiotic bacterium according to claim 1 is characterized in that, described bacterial culture fluid is to add the milk powder 1.5wt% that contains probiotic bacterium, potassium hydrogen phosphate 0.06wt%, vitamins B in water
20.01wt%, the rear sterilization that stirs makes.
5. a kind of immobilization probiotic bacterium of being rich in simultaneously vegetable fibre and probiotic bacterium according to claim 1 is characterized in that, described bacterial culture fluid is the aqueous solution of commercially available probiotic bacterium, and mass percent concentration is 1-2%.
6. a kind of immobilization probiotic bacterium of being rich in simultaneously vegetable fibre and probiotic bacterium according to claim 1 is characterized in that, the particle diameter of described microcapsule is 1～5 μ m.
7. preparation method who is rich in simultaneously the immobilization probiotic bacterium of vegetable fibre and probiotic bacterium as claimed in claim 1, it is characterized in that, the method may further comprise the steps: 8-12h at normal temperatures is soaked in water pure cereal seed, defibrination, centrifugation macrobead throw out again, the insoluble composition of getting soluble part and suspending carries out self-emulsifying to be processed, regulating the pH value is 7.2-7.5, surface of microcapsule is electronegative in the solution after self-emulsifying is processed, the cereal colloidal solution that obtains is become fine-particle powder, being fixed probiotic products with bacterial culture fluid mixing postlyophilization.
8. the preparation method who is rich in simultaneously the immobilization probiotic bacterium of vegetable fibre and probiotic bacterium according to claim 7, it is characterized in that, the cereal colloidal solution that described cereal colloidal solution is solid 40%, the viable count of described bacterial culture fluid＞1000/ gram.
9. the preparation method who is rich in simultaneously the immobilization probiotic bacterium of vegetable fibre and probiotic bacterium according to claim 7, it is characterized in that, the pH value that described self-emulsifying is treated to the insoluble composition of regulating described soluble part and suspending is 7-8, and in homogenizer, stir 10-30min, make that protein transfers to meta-alkalescence in the cereal, under the effect of high speed shear power, form albumen-grease microcapsule with the contained grease of cereal itself; Described cryodesiccated temperature is-40 ℃～0 ℃.
10. according to claim 7 or the 9 described preparation methods that are rich in simultaneously the immobilization probiotic bacterium of vegetable fibre and probiotic bacterium, it is characterized in that, described pH value adopts sodium bicarbonate to regulate.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018082665A1 (en) * | 2016-11-04 | 2018-05-11 | 华为技术有限公司 | Frequency synchronization method and slave clock |
| CN108113004A (en) * | 2017-12-15 | 2018-06-05 | 石家庄君乐宝乳业有限公司 | The preparation method of high activity Lactobacillus paracasei N1115 instant type probiotic powders |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20010016220A1 (en) * | 1999-12-22 | 2001-08-23 | Nutrinova Nutrition Specialties & Food Ingredients Gmbh | Encapsulated multifunctional biologically active food component, process for its production and its use |
| CN101534658A (en) * | 2006-10-06 | 2009-09-16 | 先进生物技术学院 | Pre-fermented symbiotic matrix based on cereal suspension containing encapsulated probiotics, method for preparing same and related applications |
| CN101933620A (en) * | 2009-07-01 | 2011-01-05 | 何黎明 | Probiotic powder and preparation method |
| CN102919669A (en) * | 2012-10-12 | 2013-02-13 | 上海致一食品有限公司 | Method for preparing immobilization probiotics by taking grain powder as embedding material |
-
2012
- 2012-11-29 CN CN2012105010710A patent/CN103013970A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20010016220A1 (en) * | 1999-12-22 | 2001-08-23 | Nutrinova Nutrition Specialties & Food Ingredients Gmbh | Encapsulated multifunctional biologically active food component, process for its production and its use |
| CN101534658A (en) * | 2006-10-06 | 2009-09-16 | 先进生物技术学院 | Pre-fermented symbiotic matrix based on cereal suspension containing encapsulated probiotics, method for preparing same and related applications |
| CN101933620A (en) * | 2009-07-01 | 2011-01-05 | 何黎明 | Probiotic powder and preparation method |
| CN102919669A (en) * | 2012-10-12 | 2013-02-13 | 上海致一食品有限公司 | Method for preparing immobilization probiotics by taking grain powder as embedding material |
Non-Patent Citations (1)
| Title |
|---|
| 谭斌: "功能性谷物基质非乳性益生菌的研究展望", 《中国食品添加剂》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018082665A1 (en) * | 2016-11-04 | 2018-05-11 | 华为技术有限公司 | Frequency synchronization method and slave clock |
| CN108113004A (en) * | 2017-12-15 | 2018-06-05 | 石家庄君乐宝乳业有限公司 | The preparation method of high activity Lactobacillus paracasei N1115 instant type probiotic powders |
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Application publication date: 20130403 |