CN107594109A - A kind of pet drinks enzyme liquid and preparation method thereof - Google Patents
A kind of pet drinks enzyme liquid and preparation method thereof Download PDFInfo
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- CN107594109A CN107594109A CN201710733521.1A CN201710733521A CN107594109A CN 107594109 A CN107594109 A CN 107594109A CN 201710733521 A CN201710733521 A CN 201710733521A CN 107594109 A CN107594109 A CN 107594109A
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Abstract
The invention discloses a kind of pet to drink enzyme liquid and preparation method thereof, is that compound fruit and vegetable stoste is made in raw material specifically by apple, banana, dragon fruit, lycium ruthenicum, lemon, onion, allocates addition oligosaccharide, vitamin, honey etc. after filtering again, dispense and produce after homogeneous.The pectase liquid of the present invention, various extracellular ferment it is energetic, each component reasonable mixture ratio, obvious synergy between each component, can be obviously promoted internal metabolism, the food in enteron aisle can effectively be decomposed, enteron aisle rubbish is removed, maintains the beneficial microbe colony of enteron aisle, and simple production process is easy to operate, suitable for industrialized production, there is good economic benefit and social benefit.
Description
Technical field
The present invention relates to Vegetable Drink Fermented technical field, and in particular to a kind of fruits and vegetables with regulation gastrointestinal function reconcile
Enzyme liquid and preparation method thereof.
Background technology
Ferment, also known as enzyme, it is a kind of protein, is the catalyst of various biochemical reactions.It is present in various work
Cell interior so that cell shows various biological phenomenas.Various metabolism in human body or animal body, caloric intake, growth
With breeding etc. must could all be completed by the help of ferment.
In general, plant enzyme is using fresh fruit of vegetables as raw material, is generated through a variety of probiotics fermentions, is that one kind has
The microorganism formulation of abundant enzyme, vitamin, mineral matter and secondary metabolite.Common fruit, vegetables, brown rice, mushroom class, medicine
The raw material that homologous Chinese medicine etc. can ferment as ferment is eaten, protease, lipase and superoxide dismutase etc. are enzyme microbs
The primary efficacy enzyme of class health food.Pectase is always the best seller in ferment class product, and it is to pass through fresh fruit of vegetables
Spontaneous fermentation develops, and remains the nutrition elite in fruits and vegetables, containing various ferment oligosaccharides and a variety of vitamins and mineral matter,
Also there is abundant SOD antioxidase compositions, the oxidation resistance of human body or animal, and then strengthen immunity can be effectively improved.
In terms of pet drinks enzyme liquid, the bioactive ingredients that contain in ferment can remove peculiar smell of pet and stink,
Promote digestive function, play antioxidation, and maintain animal intestinal tract healthy.Ferment the probiotics used and fruits and vegetables, both
Side effect is all almost nil.Therefore, apple, banana, dragon fruit, lycium ruthenicum, lemon, the mixing enzyme stoste that onion is formed are
Safe healthcare product with vitality.
The preparation of ferment can then be provided simultaneously with the effect of a variety of magical if selected for suitable food materials and zymotechnique.
The production method of traditional enzyme stoste is because its selection, proportioning, temperature setting, manufacture craft design etc. are not reasonable, so as to deposit
Function is single, poor taste, health-care effect is undesirable, product fraction defective is high the defects of.Existing ferment simple production process, production
Cycle is very long, and also has the following disadvantages：a）Easily cause the growth of spoilage organisms or go mouldy, easily eating by mistake causes in food
Poison；b）Enzyme activity composition can not be effectively retained；c）Fermentation time can not be effectively controlled, causes quality can not management and control；d）It is existing
Some pectase nutritional ingredients are single, it is impossible to meet the needs of for nutrition.
The content of the invention
For above shortcomings in the prior art, the invention provides a kind of pet to drink enzyme liquid and its preparation side
Method, there is regulation gastrointestinal function, promote digestion and remove peculiar smell of pet and stink and other effects.
The invention provides the preparation method that a kind of pet drinks enzyme liquid, comprise the following steps：
（1）By apple 10-20 parts, banana 10-20 parts, dragon fruit 35-40 parts, lycium ruthenicum 10-15 parts, lemon 5-10 parts, onion
2-4 parts are cleaned, and ozonization, peeling is cut into small pieces, and are fitted into after smashing in sterile reactor, are the super of 85-90KHz by frequency
Sound wave, processing 30-50min obtains slurries at 50-55 DEG C；
（2）Nitrogen is passed through into fermentation tank and is allowed to saturation, step 1 gained slurries are added in fermentation tank, sequentially add grape
5 parts of sugar, 5 parts of enzymatic activity agent, 25 parts of bacterium solution A, stirs, primary fermentation 10-15 days at 28-35 DEG C；Wherein, bacterium solution A by
The raw material composition of following mass percents：Water 90%, soy meal 7%, saccharomycete 2%, lactic acid bacteria 1%；
（3）Filtered by 0.4 μm of the filter in aperture, obtain the primary fermentation liquid obtained by step 2, sequentially added in filtrate thin
3 parts of lotus alcohol, 25 parts of bacterium solution B, secondary fermentation 10-15 days at 32-35 DEG C；Wherein, bacterium solution B by following mass percents original
Material composition：Water 85%, soy meal 7%, Bifidobacterium 3%, lactobacillus bulgaricus 2%, lactobacillus acidophilus 1%, Lactobacillus plantarum 2%；
（4）Step 3 gained secondary fermentation liquid is sealed into static fermentation 30-35 days at normal temperatures, 8 DEG C is reduced the temperature to, terminates hair
Ferment, and carry out after fermentation is sealed at this temperature, filtered by 0.4 μm of the filter in aperture, produce enzyme stoste；
（5）3 parts of oligosaccharide is weighed, adds purified water, mass fraction 10-15% oligosaccharide solution is made；
（6）Weigh 4 parts of cornstarch, the mixture that 3 parts of maltodextrin, with purified water according to 1:10 quality than wiring solution-forming,
Step 5 gained oligosaccharide solution is added, is well mixed, 100 DEG C of boiling 30min sterilizings is heated to, naturally cools to room temperature, obtain mixed
Close liquid；
（7）Step 4 gained enzyme stoste is added in step 6 gained mixed liquor, is filtered by 0.4 μm of the filter in aperture, then
1 part of nutrient is added, 2 parts of honey, 0.1 part of thickener, is well mixed, the homogeneous under the conditions of 35-45 DEG C and 20Mpa, is then put
Sterilize 30min in 55-60 DEG C, is then cooled to 15-20 DEG C of progress sterile filling, and last normal temperature is kept in dark place, produced.
Preferably, the enzymatic activity agent described in step 2 is mixed by Radix Rhapontici seu Radix Echinopsis sugar, xylitol, lipoic acid and guar gum, institute
State Radix Rhapontici seu Radix Echinopsis sugar：Xylitol：Lipoic acid：The mass ratio of guar gum is（3-4）：（1-2）：（2-3）：（0.5-1）.
It is further preferred that the lipoic acid is the crystal formation I of R (+)-lipoic acid -1B salt, radiated using Cu-K α, its
In the collection of illustrative plates of X-ray powder diffraction, 2 θ values 5.102,7.589,10.072,12.549,15.036,16.688,18.627,
19.356th, there is diffraction maximum at 25.064,27.611, wherein 2 θ values error ranges are ± 0.2.
Preferably, oligosaccharide described in step 5 is selected from：FOS, xylo-oligosaccharide, galactooligosaccharide, chitosan are oligomeric sweet
Dew is sugared, raffinose, soyabean oligosaccharides, kanjak mannan-oligosaccharides, palatinose, the one or more in inulin.
It is further preferred that the oligosaccharide is selected from the mixture of raffinose and kanjak mannan-oligosaccharides, weight ratio is 1:1.
Preferably, nutrient is selected from：Vitamin A, vitamin B complex, vitamin C, vitamin D, vitamin E, folic acid, sulfuric acid
Zinc, calcium lactate, the one or more in magnesium gluconate.
It is further preferred that nutrient is selected from vitamin B complex.
Preferably, thickener described in step 7 is selected from sodium carboxymethylcellulose, xanthans, sodium alginate, in guar gum
It is one or more of.
It is further preferred that thickener is selected from sodium alginate.
Preferably, the ozone sterilization condition described in step 1 is as follows：Ozone concentration 30-50mg/m3, time 2-3h.
The present invention achieves following beneficial technique effect：
（1）Before fermentation, to needing the fruits and vegetables for preparing enzyme stoste to carry out ultrasonic assistant heating so that nutriment obtains
Farthest extraction；Temperature controlled fermentation simultaneously, can so ensure the quality and quantity of the probioticses of advantage, and promote its a large amount of
Breeding, reaches the purpose of Rapid Fermentation, while can also reduce the generation of bad flavor and harmful substance, greatly improves zymotic fluid
Quality, the vigor of strain can be kept by terminating fermentation using low temperature after fermentation ends, while using low temperature progress after fermentation, can be promoted
The fusion of fruity and fermentation perfume, makes fragrance more plentiful, tart flavour is softer, and mouthfeel is more coordinated, and drastically increases quality；Low temperature cold
Hide, ensure that the quality and quantity of wherein probiotics viable bacteria.Meanwhile the use of the distinctive enzymatic activity agent of the present invention so that fermentation
Process is smoothed out, and zymotic fluid quality is higher.
（2）The ferment uniform color, has strong fruity and the fragrance that ferments, mellow in taste, comfortable acid, entrance do not pierce
Swash, pleasant impression is sweet.
（3）The effect of pet's oral cavity peculiar smell can be removed：Animal oral cavity peculiar smell is residual mainly due to digestion power deficiency, food
Corruption is produced after staying.Banana contains abundant dietary fiber, and its a big chunk will not be digested and absorb, but gram cause excrement
Cubical content increase, and promote enterocinesia.Meanwhile the sugar content of banana is more than 15%, and contain a large amount of water-solubility vegetable fibres, energy
Cause the gastro-intestinal Fluid of hypertonicity to be secreted, excrement is softened and is easily discharged.And saccharomycete and lactic acid bacteria energy extracellular proteinase and fat
Fat enzyme, promote digestive function.
（4）Anti-oxidation function：The polyphenol and flavonoids native chemical polyphenoils that contain in apple and substantial amounts of crude fibre.
A large amount of SOD enzymes can also be produced in ferment, caused active oxygen in animal body can be eliminated, produce antioxidation.
（5）Maintain intestinal health effect：Zymophyte produces a large amount of organic acids, reduces intestinal pH, improves above-mentioned enzymatic activity, together
When promote beneficial to bacteria growing, suppress harmful bacteria growing.The present invention can also have fall fire by Bifidobacteria population, and profit just, improves new
Old metabolism, intestines peristalsis can be promoted and discharge stool, had no side effect safely.
（6）Oligosaccharide used in the present invention, it is that one kind is not degraded by human body hydrochloric acid in gastric juice, gastric enzyme, not in intestinal absorption, and can
Reach the oligosaccharide of big intestines.The oligosaccharide is the substrate of growth of probiotics, has the physiology such as the propagation for promoting human body probiotics
Function.Probiotics and functional oligose are used in conjunction with, and synergy, promote the regulatory function to gut flora, both are mutually auxiliary
Coordinate, play a role jointly, promote beneficial to proliferation of probiotics, suppress harmful bacteria growing.
（7）Ferment of the present invention is rich in multivitamin, amino acid, Soluble plant fibers and trace element, has and mitigates
Enteron aisle load, healthy cycle system is activated, adjust microecological balance.Cornstarch therein, generated after acid hydrolyzes with ferment
Water-soluble fibre, its fiber content are up to 80%, improve constipation, help intestinal health.Added in inventive formulation a certain proportion of
It maltodextrin, can not only improve nutrition-allocated proportion, and beneficiating ingredient is more easily absorbed.
（8）Fruits and vegetables used in the present invention are also well-chosen, and benefit is the more：
Lemon is that have one of fruit of medical value, and the traditional Chinese medical science thinks that lemon has heat-clearing, sterilization, appetizing, resolving sputum, cough-relieving
Effect, also moisturizing, beauty treatment, eliminate peculiar smell the effect of, doctor trained in Western medicine think vitamin C improve body immunity, prevent scurvy；Dimension
Raw plain P is the conditioning agent of human microvascular, prevents human vas from hardening, anti-hemostatic tube hemorrhage.
Hylocereus undatus is nutritious, rich in various vitamins, mineral matter, anthocyanidin, flavonoids, saponin(e, butanedioic acid, water-soluble meals
Fiber is eaten, is the sage of fruit, there are the functions such as hypotensive, reducing blood lipid, removing toxic substances, moistening lung, dragon fruit also has viscosity containing a kind of
Vegetable albumin, heavy metal has chelant and the function of discharge, also has protective effect to coat of the stomach.
Anthocyanidin is rich in lycium ruthenicum.Anthocyanidin is the water-soluble natural color that nature one kind is widely present in plant
Element, there is anti-oxidant, anti-inflammatory, reducing blood lipid, suppress the physiological functions such as tumor proliferative.The preparation method of the present invention can be to greatest extent
The activity and function of anthocyanidin are remained, free radical unnecessary in human body can be neutralized, there is anti-oxidant, anti-aging, is alleviated tired
Labor, it is suppressed that the growth of spoilage organisms, remain the original flavor of ferment, nutrition and active function, improve the ferment shelf-life, have
There is wide market development prospect.
Embodiment
Embodiment 1
（1）By 20 parts of apple, 15 parts of banana, 35 parts of dragon fruit, 10 parts of lycium ruthenicum, 5 parts of lemon, 3 parts of onion, clean, ozone disappears
Poison, ozone concentration 30-50mg/m3, time 2-3h, peeling is cut into small pieces, is fitted into after smashing in sterile reactor, passes through frequency
For 85KHz ultrasonic wave, processing 30min obtains slurries at 50-55 DEG C；
（2）Nitrogen is passed through into fermentation tank and is allowed to saturation, step 1 gained slurries are added in fermentation tank, sequentially add grape
5 parts of sugar, 5 parts of enzymatic activity agent, 25 parts of bacterium solution A, stirs, primary fermentation 10-15 days at a temperature of 28-35 DEG C；Wherein, its
Middle enzymatic activity agent is by Radix Rhapontici seu Radix Echinopsis sugar：Xylitol：The crystal formation I of R (+)-lipoic acid -1B salt：Guar gum, according to mass ratio
3：1：2：Being formed mixed by 0.5, the crystal formation I of described R (+)-lipoic acid -1B salt refers to radiate using Cu-K α, its
In the collection of illustrative plates of X-ray powder diffraction, 2 θ values 5.102,7.589,10.072,12.549,15.036,16.688,18.627,
19.356th, there is diffraction maximum at 25.064,27.611, wherein 2 θ values error ranges are ± 0.2；Bacterium solution A is by following mass percents
Raw material composition：Water 90%, soy meal 7%, saccharomycete 2%, lactic acid bacteria 1%；
（3）Filtered by 0.4 μm of the filter in aperture, obtain the primary fermentation liquid obtained by step 2, menthol is added in filtrate
3 parts, 25 parts of bacterium solution B, secondary fermentation 10-15 days in 32-35 DEG C of temperature；Wherein, bacterium solution B is by following mass percents
Raw material forms：Water 85%, soy meal 7%, Bifidobacterium 3%, lactobacillus bulgaricus 2%, lactobacillus acidophilus 1%, Lactobacillus plantarum
2%；
（4）Step 3 gained secondary fermentation liquid is sealed into static fermentation 30-35 days at normal temperatures, 8 DEG C is reduced the temperature to, terminates hair
Ferment, and carry out after fermentation is sealed at this temperature, filtered by 0.4 μm of the filter in aperture, produce enzyme stoste；
（5）1.5 parts of raffinose is weighed, 1.5 parts of kanjak mannan-oligosaccharides, adds purified water, the oligosaccharide of mass fraction 10% is made
Solution；
（6）Weigh 4 parts of cornstarch, the mixture that 3 parts of maltodextrin, with purified water according to 1:10 quality than wiring solution-forming,
Step 5 gained oligosaccharide solution is added, is well mixed, 100 DEG C of boiling 30min sterilizings is heated to, naturally cools to room temperature, obtain mixed
Close liquid；
（7）Step 4 gained enzyme stoste is added in step 6 gained mixed liquor, is filtered by 0.4 μm of the filter in aperture, then
1 part of vitamin B complex is added, 2 parts of honey, 0.1 part of sodium alginate, is well mixed, the homogeneous under the conditions of 35-45 DEG C and 20Mpa, is connect
And be placed in 55-60 DEG C of sterilizing 30min, be then cooled to 15-20 DEG C of progress sterile filling, last normal temperature is kept in dark place, produced.
Embodiment 2
（1）By 10 parts of apple, 20 parts of banana, 40 parts of dragon fruit, 15 parts of lycium ruthenicum, 10 parts of lemon, 2 parts of onion, clean, ozone disappears
Poison, ozone concentration 30-50mg/m3, time 2-3h, peeling is cut into small pieces, is fitted into after smashing in sterile reactor, passes through frequency
For 90KHz ultrasonic wave, processing 50min obtains slurries at 50-55 DEG C；
（2）Nitrogen is passed through into fermentation tank and is allowed to saturation, step 1 gained slurries are added in fermentation tank, sequentially add grape
5 parts of sugar, 5 parts of enzymatic activity agent, 25 parts of bacterium solution A, stirs, primary fermentation 10-15 days at a temperature of 28-35 DEG C；Wherein, its
Middle enzymatic activity agent is by Radix Rhapontici seu Radix Echinopsis sugar：Xylitol：The crystal formation I of R (+)-lipoic acid -1B salt：Guar gum, according to mass ratio
4：2：3：Formed mixed by 1, the crystal formation I of described R (+)-lipoic acid -1B salt refers to radiate using Cu-K α, its X-
In the collection of illustrative plates of ray powder diffraction, 2 θ values 5.102,7.589,10.072,12.549,15.036,16.688,18.627,
19.356th, there is diffraction maximum at 25.064,27.611, wherein 2 θ values error ranges are ± 0.2；Bacterium solution A is by following mass percents
Raw material composition：Water 90%, soy meal 7%, saccharomycete 2%, lactic acid bacteria 1%；
（3）Filtered by 0.4 μm of the filter in aperture, obtain the primary fermentation liquid obtained by step 2, menthol is added in filtrate
3 parts, 25 parts of bacterium solution B, secondary fermentation 10-15 days in 32-35 DEG C of temperature, wherein, bacterium solution B is by following mass percents
Raw material forms：Water 85%, soy meal 7%, Bifidobacterium 3%, lactobacillus bulgaricus 2%, lactobacillus acidophilus 1%, Lactobacillus plantarum
2%；
（4）Step 3 gained secondary fermentation liquid is sealed into static fermentation 30-35 days at normal temperatures, 8 DEG C is reduced the temperature to, terminates hair
Ferment, and carry out after fermentation is sealed at this temperature, filtered by 0.4 μm of the filter in aperture, produce enzyme stoste；
（5）3 parts of raffinose is weighed, adds purified water, the oligosaccharide solution of mass fraction 15% is made；
（6）Weigh 4 parts of cornstarch, the mixture that 3 parts of maltodextrin, with purified water according to 1:10 quality than wiring solution-forming,
Step 5 gained oligosaccharide solution is added, is well mixed, 100 DEG C of boiling 30min sterilizings is heated to, naturally cools to room temperature, obtain mixed
Close liquid；
（7）Step 4 gained enzyme stoste is added in step 6 gained mixed liquor, is filtered by 0.4 μm of the filter in aperture, then
1 part of vitamin B complex is added, 2 parts of honey, 0.1 part of sodium alginate, is well mixed, the homogeneous under the conditions of 35-45 DEG C and 20Mpa, is connect
And be placed in 55-60 DEG C of sterilizing 30min, be then cooled to 15-20 DEG C of progress sterile filling, last normal temperature is kept in dark place, produced.
Embodiment 3
（1）By 15 parts of apple, 15 parts of banana, 35 parts of dragon fruit, 10 parts of lycium ruthenicum, 5 parts of lemon, 3 parts of onion, clean, ozone disappears
Poison, ozone concentration 30-50mg/m3, time 2-3h, peeling is cut into small pieces, is fitted into after smashing in sterile reactor, passes through frequency
For 85KHz ultrasonic wave, processing 40min obtains slurries at 50-55 DEG C；
（2）Nitrogen is passed through into fermentation tank and is allowed to saturation, step 1 gained slurries are added in fermentation tank, sequentially add grape
5 parts of sugar, 5 parts of enzymatic activity agent, 25 parts of bacterium solution A, stirs, primary fermentation 10-15 days at a temperature of 28-35 DEG C；Wherein, its
Middle enzymatic activity agent is by Radix Rhapontici seu Radix Echinopsis sugar：Xylitol：R (+)-lipoic acid：Guar gum, according to mass ratio 3：2：2：Formed mixed by 1
, described R (+)-lipoic acid refers to R-DHLA；Bacterium solution A is made up of the raw material of following mass percents：Water 90%, soybean
Powder 7%, saccharomycete 2%, lactic acid bacteria 1%；
（3）Filtered by 0.4 μm of the filter in aperture, obtain the primary fermentation liquid obtained by step 2, menthol is added in filtrate
3 parts, 25 parts of bacterium solution B, secondary fermentation 10-15 days in 32-35 DEG C of temperature, wherein, bacterium solution B is by following mass percents
Raw material forms：Water 85%, soy meal 7%, Bifidobacterium 3%, lactobacillus bulgaricus 2%, lactobacillus acidophilus 1%, Lactobacillus plantarum
2%；
（4）Step 3 gained secondary fermentation liquid is sealed into static fermentation 30-35 days at normal temperatures, 8 DEG C is reduced the temperature to, terminates hair
Ferment, and carry out after fermentation is sealed at this temperature, filtered by 0.4 μm of the filter in aperture, produce enzyme stoste；
（5）3 parts of kanjak mannan-oligosaccharides are weighed, add purified water, the oligosaccharide solution of mass fraction 10% is made；
（6）Weigh 4 parts of cornstarch, the mixture that 3 parts of maltodextrin, with purified water according to 1:10 quality than wiring solution-forming,
Step 5 gained oligosaccharide solution is added, is well mixed, 100 DEG C of boiling 30min sterilizings is heated to, naturally cools to room temperature, obtain mixed
Close liquid；
（7）Step 4 gained enzyme stoste is added in step 6 gained mixed liquor, is filtered by 0.4 μm of the filter in aperture, then
1 part of vitamin C is added, 2 parts of honey, 0.1 part of xanthans, is well mixed, the homogeneous under the conditions of 35-45 DEG C and 20Mpa, then
55-60 DEG C of sterilizing 30min is placed in, is then cooled to 15-20 DEG C of progress sterile filling, last normal temperature is kept in dark place, produced.
Embodiment 4
（1）By 10 parts of apple, 20 parts of banana, 40 parts of dragon fruit, 15 parts of lycium ruthenicum, 8 parts of lemon, 3 parts of onion, clean, ozone disappears
Poison, ozone concentration 30-50mg/m3, time 2-3h, peeling is cut into small pieces, is fitted into after smashing in sterile reactor, passes through frequency
For 85KHz ultrasonic wave, processing 50min obtains slurries at 50-55 DEG C；
（2）Nitrogen is passed through into fermentation tank and is allowed to saturation, step 1 gained slurries are added in fermentation tank, sequentially add grape
5 parts of sugar, 5 parts of enzymatic activity agent, 25 parts of bacterium solution A, stirs, primary fermentation 10-15 days at a temperature of 28-35 DEG C；Wherein, its
Middle enzymatic activity agent is by Radix Rhapontici seu Radix Echinopsis sugar：Xylitol：R (+)-lipoic acid：Guar gum, according to mass ratio 3：2：2：Formed mixed by 1
, described R (+)-lipoic acid refers to racemization lipoic acid；Bacterium solution A is made up of the raw material of following mass percents：Water 90%, soybean
Powder 7%, saccharomycete 2%, lactic acid bacteria 1%；
（3）Filtered by 0.4 μm of the filter in aperture, obtain the primary fermentation liquid obtained by step 2, menthol is added in filtrate
3 parts, 25 parts of bacterium solution B, secondary fermentation 10-15 days in 32 DEG C -35 DEG C of temperature, wherein, bacterium solution B is by following mass percents
Raw material composition：Water 85%, soy meal 7%, Bifidobacterium 3%, lactobacillus bulgaricus 2%, lactobacillus acidophilus 1%, Lactobacillus plantarum
2%；
（4）Step 3 gained secondary fermentation liquid is sealed into static fermentation 30-35 days at normal temperatures, 8 DEG C is reduced the temperature to, terminates hair
Ferment, and carry out after fermentation is sealed at this temperature, filtered by 0.4 μm of the filter in aperture, produce enzyme stoste；
（5）3 parts of kanjak mannan-oligosaccharides are weighed, add purified water, the oligosaccharide solution of mass fraction 15% is made；
（6）Weigh 4 parts of cornstarch, the mixture that 3 parts of maltodextrin, with purified water according to 1:10 quality than wiring solution-forming,
Step 5 gained oligosaccharide solution is added, is well mixed, 100 DEG C of boiling 30min sterilizings is heated to, naturally cools to room temperature, obtain mixed
Close liquid；
（7）Step 4 gained enzyme stoste is added in step 6 gained mixed liquor, is filtered by 0.4 μm of the filter in aperture, then
1 part of vitamin E is added, 2 parts of honey, 0.1 part of sodium carboxymethylcellulose, is well mixed, under the conditions of 35-45 DEG C and 20Mpa
Matter, 55-60 DEG C of sterilizing 30min is then placed in, is then cooled to 15-20 DEG C of progress sterile filling, last normal temperature is kept in dark place, i.e.,
.
The enzyme activity determination of test example 1 is tested
The present inventor uses prolease activity in determined by ultraviolet spectrophotometry pectase, is surveyed using assay NBT photoreduction
Determine ferment SOD vigor, amylase activity is determined using iodine colorimetry, using titration measuring lipase activity.
Comparative example used in this test example is prepared according to the raw material proportioning described in embodiment 1 and method, but is not added
Enzymatic activity agent processing.
From the foregoing, it will be observed that the ferment of the present invention for adding enzyme activity agent is compared with comparative example, and in protease, amylase, lipase,
And occupy clear superiority in the enzymatic activity index of superoxide dismutase.
Test example 2 adjusts the experiment of Bacteria from Gl Tract of Rats
Regulation experiment to Bacteria from Gl Tract of Rats, it is specific as follows to prove that the pectase has the effect of coordinating intestines and stomach：Weigh
SD rats 40, male and female half and half, body weight 180-220g, four groups are randomly divided into, every group 10, enteron aisle is made with Lincomycin Hydrochloride
The model of flora imbalance, then it is divided into following four groups：Control group 1, control group 2, embodiment group, and natural recovering group.
Given the test agent is the pectase of the gained of embodiment 1.Tested first three groups are respectively daily according to 0.5mL/Kg bw agent
Amount gavage, each gavage capacity 0.1mL/100g bw, twice daily, and progress flora adjustment, continuous 7 days.
Compare 1 group of mixed fruit and vegetable juice using same ratio when being made with enzyme liquid of the present invention；Compare 2 groups of uses and this hair
The nutrient solution of the nutritional ingredient configuration of same ratio when bright ferment makes；Pectase of the embodiment group obtained by using embodiment 1；
Natural recovering group is filled with the physiological saline of equivalent.After gavage terminates, stool in mice 0.2-0.3g is taken with aseptic procedure, in proportion
After matter dilution, aerobic and Anaerobic culturel is carried out respectively, finally counts lactobacillus, Bifidobacterium, enterobacteria, enterococcus.
Entirety is seen, the best results obtained by embodiment group, shows that the nutritional ingredient in ferment of the present invention and ferment collaboration are made
With the growth that can promote intestinal beneficial flora.
The dinitrofluorobenzene of test example 3（DNFB）Induce delayed allergy（DTH）Experiment
Select cleaning grade mouse some, body weight 18-22g, low middle high three dosage is set, is followed successively by：3ml/kg bw, 6ml/kg
bw 18ml/kg bw.Negative control group, test group is set to be given after using the deployed concentration of distilled water simultaneously, negative control group
Directly give distilled water, gavage mouse 30 days.Given the test agent is the pectase of the gained of embodiment 1.
By prescribed dose, after each group test mice continuous gavage 30d, mouse web portion unhairing, with 1%DNFB acetone sesame oil
Solution 50uL smears sensitization, and 5d after sensitization, mouse right ear is uniformly smeared 1%DNFB acetone sesame oil solutions 10uL and attacked, left ear
The control of acetone sesame oil solution is smeared, 24h puts to death mouse after attack, cuts left and right auricular concha, takes diameter 8mm auricles to claim in same position
Weight, left and right auricle weight is only poor to be used as swelling.
With control group ratio, ferment of the present invention, low dosage influences unobvious, middle dosage and high dose pair to mice ear degree
Mice ear degree is obviously promoted rise effect, that is, ear swelling degree of the ferment of the present invention to mouse delayed allergy
With facilitation, that is, have the function that to strengthen mouse cell immunologic function.
The phagocytosis rabbit erythrocyte experiment of the Turnover of Mouse Peritoneal Macrophages of test example 4
It is some by selection cleaning grade mouse, body weight 18-22g, low middle high three dosage, 3ml/kg bw, 6ml/kg bw are set
18ml/kg bw.Negative control group, test group is set to be given after using the deployed concentration of distilled water simultaneously, negative control group is direct
Give distilled water, gavage mouse 30 days.Given the test agent is the pectase of the gained of embodiment 1.
According to prescribed dose to each group test mice continuous gavage 30 days after, hanged to every mouse peritoneal injection rabbit erythrocyte
Liquid, mouse macrophage is activated, mouse is put to death after 4 days, Hank ' the s solution 4mL/ of intraperitoneal injection addition calf serum is only.Inhale
1% rabbit erythrocyte of abdominal cavity washing lotion and equivalent is taken to mix.0.5mL mixed liquors are drawn, adds in the agar circle of slide, is placed in 37 DEG C
Incubator in be incubated 20min, will not glue parietal cell using physiological saline after terminating and wash out, in methyl alcohol fixed 1min, Giemsa
Liquid dyes 15min, and with 50X microscopic counting phagocytic rates, phagocytic rate is in every 100 macrophages, swallows the huge of rabbit erythrocyte
Percentage shared by phagocyte, and the phagocytic activity of macrophage is judged accordingly.
As seen from the above table, compared with control group, the low middle height of the present invention is gulped down to Turnover of Mouse Peritoneal Macrophages phagocytosis rabbit erythrocyte
There is the rate of biting obvious rise to act on（P ＜ 0.05）, illustrate that thing of the present invention has the function that to promote mouse phagocyte, can strengthen
Its non-specific immune function.
Claims (10)
1. a kind of pet drinks the preparation method of enzyme liquid, it is characterised in that comprises the following steps：
（1）By apple 10-20 parts, banana 10-20 parts, dragon fruit 35-40 parts, lycium ruthenicum 10-15 parts, lemon 5-10 parts, onion
2-4 parts are cleaned, and ozonization, peeling is cut into small pieces, and are fitted into after smashing in sterile reactor, are the super of 85-90KHz by frequency
Sound wave, processing 30-50min obtains slurries at 50-55 DEG C；
（2）Nitrogen is passed through into fermentation tank and is allowed to saturation, step 1 gained slurries are added in fermentation tank, sequentially add grape
5 parts of sugar, 5 parts of enzymatic activity agent, 25 parts of bacterium solution A, stirs, primary fermentation 10-15 days at 28-35 DEG C；Wherein, bacterium solution A by
The raw material composition of following mass percents：Water 90%, soy meal 7%, saccharomycete 2%, lactic acid bacteria 1%；
（3）Filtered by 0.4 μm of the filter in aperture, obtain the primary fermentation liquid obtained by step 2, sequentially added in filtrate thin
3 parts of lotus alcohol, 25 parts of bacterium solution B, secondary fermentation 10-15 days at 32-35 DEG C；Wherein, bacterium solution B by following mass percents original
Material composition：Water 85%, soy meal 7%, Bifidobacterium 3%, lactobacillus bulgaricus 2%, lactobacillus acidophilus 1%, Lactobacillus plantarum 2%；
（4）Step 3 gained secondary fermentation liquid is sealed into static fermentation 30-35 days at normal temperatures, 8 DEG C is reduced the temperature to, terminates hair
Ferment, and carry out after fermentation is sealed at this temperature, filtered by 0.4 μm of the filter in aperture, produce enzyme stoste；
（5）3 parts of oligosaccharide is weighed, adds purified water, mass fraction 10-15% oligosaccharide solution is made；
（6）Weigh 4 parts of cornstarch, the mixture that 3 parts of maltodextrin, with purified water according to 1:10 quality than wiring solution-forming,
Step 5 gained oligosaccharide solution is added, is well mixed, 100 DEG C of boiling 30min sterilizings is heated to, naturally cools to room temperature, obtain mixed
Close liquid；
（7）Step 4 gained enzyme stoste is added in step 6 gained mixed liquor, is filtered by 0.4 μm of the filter in aperture, then
1 part of nutrient is added, 2 parts of honey, 0.1 part of thickener, is well mixed, the homogeneous under the conditions of 35-45 DEG C and 20Mpa, is then put
Sterilize 30min in 55-60 DEG C, is then cooled to 15-20 DEG C of progress sterile filling, and last normal temperature is kept in dark place, produced.
2. preparation method according to claim 1, it is characterised in that the enzymatic activity agent described in step 2 is by Radix Rhapontici seu Radix Echinopsis sugar, xylose
Alcohol, lipoic acid and guar gum mix, the Radix Rhapontici seu Radix Echinopsis sugar：Xylitol：Lipoic acid：The mass ratio of guar gum is（3-4）
：（1-2）：（2-3）：（0.5-1）.
3. preparation method according to claim 2, it is characterised in that the lipoic acid is R (+)-lipoic acid -1B
The crystal formation I of salt, it is characterised in that radiated using Cu-K α, in the collection of illustrative plates of its X-ray powder diffraction, 2 θ values 5.102,
7.589th, there is diffraction maximum at 10.072,12.549,15.036,16.688,18.627,19.356,25.064,27.611, wherein 2
θ values error range is ± 0.2.
4. preparation method according to claim 1, it is characterised in that oligosaccharide is selected from described in step 5：FOS is low
Xylan, galactooligosaccharide, chitosan, Oligomeric manna sugar, raffinose, soyabean oligosaccharides, kanjak mannan-oligosaccharides, para gold
Sugar, the one or more in inulin.
5. preparation method according to claim 4, it is characterised in that the oligosaccharide is selected from raffinose and kanjak mannan is low
The mixture of glycan, weight ratio are 1:1.
6. preparation method according to claim 1, it is characterised in that nutrient is selected from described in step 7：Vitamin A, dimension life
Plain B races, vitamin C, vitamin D, vitamin E, folic acid, zinc sulfate, calcium lactate, the one or more in magnesium gluconate.
7. preparation method according to claim 6, it is characterised in that nutrient is selected from vitamin B complex.
8. preparation method according to claim 1, it is characterised in that thickener described in step 7 is selected from carboxymethyl cellulose
Sodium, xanthans, sodium alginate, the one or more in guar gum.
9. preparation method according to claim 8, it is characterised in that thickener is selected from sodium alginate.
10. preparation method according to claim 1, it is characterised in that the ozone sterilization condition described in step 1 is as follows：It is smelly
Oxygen concentration 30-50mg/m3, time 2-3h.
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| CN108782947A (en) * | 2018-05-24 | 2018-11-13 | 彭家煌 | A kind of ornamental fish eats the preparation method of ferment |
| CN108902017A (en) * | 2018-06-08 | 2018-11-30 | 陕西正能农牧科技有限责任公司 | The one boar cultivation fattening later period adds feed producing method without vitamin |
| CN112006167A (en) * | 2020-09-18 | 2020-12-01 | 上海一点不麻烦生物科技有限公司 | Lemon-flavored pet natural enzymolysis water containing small molecular peptides |
| CN112006168A (en) * | 2020-09-18 | 2020-12-01 | 上海一点不麻烦生物科技有限公司 | Blueberry-flavored pet natural enzymolysis water containing small molecular peptides |
| CN112616993A (en) * | 2020-12-25 | 2021-04-09 | 黑龙江省京福龙农牧科技开发有限公司 | Enzyme for pets and preparation method thereof |
| CN116584577A (en) * | 2023-05-31 | 2023-08-15 | 深圳市豆柴宠物用品有限公司 | Metazoan containing living probiotics and preparation method and application thereof |
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