CN102511474A - Production method and application of adinandra millettii cutting preservation pretreating mother solution - Google Patents
Production method and application of adinandra millettii cutting preservation pretreating mother solution Download PDFInfo
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- 238000004519 manufacturing process Methods 0.000 title claims abstract description 12
- 238000005520 cutting process Methods 0.000 title claims description 4
- 238000004321 preservation Methods 0.000 title description 2
- 241001062802 Adinandra millettii Species 0.000 title 1
- 239000010413 mother solution Substances 0.000 title 1
- 239000012452 mother liquor Substances 0.000 claims abstract description 19
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 229930006000 Sucrose Natural products 0.000 claims abstract description 8
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 8
- 239000005720 sucrose Substances 0.000 claims abstract description 8
- 229960005070 ascorbic acid Drugs 0.000 claims abstract description 7
- 235000010323 ascorbic acid Nutrition 0.000 claims abstract description 7
- 239000011668 ascorbic acid Substances 0.000 claims abstract description 7
- 239000002994 raw material Substances 0.000 claims abstract description 7
- 229920000742 Cotton Polymers 0.000 claims abstract description 6
- 239000007788 liquid Substances 0.000 claims abstract description 5
- BSYNRYMUTXBXSQ-FOQJRBATSA-N 59096-14-9 Chemical compound CC(=O)OC1=CC=CC=C1[14C](O)=O BSYNRYMUTXBXSQ-FOQJRBATSA-N 0.000 claims abstract description 3
- 239000000243 solution Substances 0.000 claims description 16
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 15
- HFCYZXMHUIHAQI-UHFFFAOYSA-N Thidiazuron Chemical compound C=1C=CC=CC=1NC(=O)NC1=CN=NS1 HFCYZXMHUIHAQI-UHFFFAOYSA-N 0.000 claims description 11
- 238000010790 dilution Methods 0.000 claims description 5
- 239000012895 dilution Substances 0.000 claims description 5
- BSYNRYMUTXBXSQ-UHFFFAOYSA-N Aspirin Chemical compound CC(=O)OC1=CC=CC=C1C(O)=O BSYNRYMUTXBXSQ-UHFFFAOYSA-N 0.000 claims description 4
- 229960001138 acetylsalicylic acid Drugs 0.000 claims description 4
- 239000012530 fluid Substances 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 3
- 238000002791 soaking Methods 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 2
- 239000003643 water by type Substances 0.000 claims 2
- 241000219000 Populus Species 0.000 abstract description 12
- 238000003860 storage Methods 0.000 abstract description 8
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 238000004806 packaging method and process Methods 0.000 abstract description 2
- 239000003755 preservative agent Substances 0.000 abstract description 2
- 230000002335 preservative effect Effects 0.000 abstract description 2
- 238000002203 pretreatment Methods 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 7
- 238000004383 yellowing Methods 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- 239000005648 plant growth regulator Substances 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 description 1
- 241001062872 Cleyera japonica Species 0.000 description 1
- 208000036828 Device occlusion Diseases 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
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- 230000015556 catabolic process Effects 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
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- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 239000004062 cytokinin Substances 0.000 description 1
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 1
- 239000002837 defoliant Substances 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
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- 235000016709 nutrition Nutrition 0.000 description 1
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- 239000003375 plant hormone Substances 0.000 description 1
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Abstract
一种杨桐切枝保鲜预处理母液生产方法,由下列原料及其重量份配比而成:A method for producing a pretreatment mother liquor of poplar cut branches and keeping fresh, comprising the following raw materials and their proportions by weight: 80-32080-320 份蔗糖、part sucrose, 0.08-0.320.08-0.32 份抗坏血酸、part ascorbic acid, 0.16-0.200.16-0.20 份乙酰水杨酸、acetylsalicylic acid, 0.08-0.120.08-0.12 份噻苯隆和thiadizuron and 40004000 份水混合而成。用上述预处理母液对杨桐切枝进行预处理时,先用四倍的水稀释成杨桐切枝保鲜液处理液,再将杨桐枝条捆成束,切口浸于预处理液中Mix with water. When using the above-mentioned pretreatment mother liquor to pretreat the Yangtong cut branches, first dilute it with four times the water to form the Yangtong cut branches fresh-keeping liquid treatment solution, then bundle the Yangtong branches into bundles, and immerse the cuts in the pretreatment solution 24h24h 后取出,用浸润有该预处理液的脱脂棉包裹切口,送至冷库低温储运。用本母液和预处理方法,比用清水和Finally, take it out, wrap the incision with absorbent cotton soaked with the pretreatment solution, and send it to a cold storage for low-temperature storage and transportation. Using this mother liquor and pretreatment method is better than using clean water and 200mg/kg200mg/kg 吲哚丁酸保鲜液处理分别延长Indolebutyric acid preservative solution treatment prolongs the 1414 天和Tianhe 1010 天的保鲜期,且成本低,生产母液节省储运和包装成本,有利于生产厂家、应用户和切枝购买户。1-day fresh-keeping period, and low cost, the production of mother liquor saves storage, transportation and packaging costs, which is beneficial to manufacturers, consumers and purchasers of cut branches.
Description
技术领域 technical field
本发明涉及一种杨桐(Cleyera japonica Thunb.)带叶枝条切下后进行预处理的保鲜母液的生产方法及应用技术。The invention relates to a production method and application technology of a fresh-keeping mother liquor prepared by cutting off leafy branches of Cleyera japonica Thunb. and performing pretreatment.
背景技术 Background technique
日本由于插花、祭祀及日常生活等活动的需要,每年都要消费大量的杨桐切枝,而且需求越来越大。每年切叶、切枝消费达350亿日元(约合人民币50亿元)左右,我国年均出口约占日本国一年总需求量的65 %,因此,杨桐切枝在日本消费市场广阔,我国利用杨桐生产可创造较大的经济效益。Due to the needs of flower arrangement, sacrifice and daily life and other activities, Japan consumes a large amount of Yangtong cut branches every year, and the demand is increasing. The annual consumption of cut leaves and branches reaches about 35 billion yen (approximately RMB 5 billion), and my country’s annual exports account for about 65% of Japan’s total annual demand. Therefore, cut branches of Yangtong have a large consumer market in Japan , my country's use of Yangtong production can create greater economic benefits.
长期以来,因杨桐枝条剪取均系当年生枝条,一般采后1-2 d,叶子开始枯萎、黄化及脱落,从我国山地采剪、整理,办出口手续后再运输到日本,时间长,因此,延长杨桐切枝的保鲜期,就成为杨桐产品出口创汇的关键。浙江凭借天然的气候优势,已成为杨桐出口的大省之一,但现今杨桐保鲜液的研究很少,且实际生产中多沿用清水进行预处理,因此研发出应用于杨桐保鲜的预处理液有一定的现实意义。For a long time, because the branches of Yangtong are cut from the branches of the same year, generally 1-2 days after harvesting, the leaves begin to wither, yellow and fall off. They are cut and sorted from the mountains in my country, and then transported to Japan after export procedures. Therefore, prolonging the fresh-keeping period of Yangtong cut branches has become the key to earning foreign exchange from the export of Yangtong products. With its natural climate advantages, Zhejiang has become one of the major export provinces of Yangtong, but there are few studies on Yangtong fresh-keeping solution, and in actual production, water is often used for pretreatment. The treatment fluid has certain practical significance.
经检索,有关杨桐切枝的保鲜文献现只有1998年江西庐山自然保护区管理处的《野生杨桐枝条保鲜技术研究简报》的一篇报道,该文中指出分别用吲哚丁酸、ABT生根粉、α-萘乙酸、2, 4- D四种植物生长调节剂作为采后储运前的处理液,24 h处理后则吲哚丁酸200 mg/kg处理效果最佳,能延长杨桐保鲜寿命达35 d。After searching, there is only a report on the fresh-keeping literature of poplar cut branches in 1998 in the "Research Briefing on Fresh-keeping Technology of Wild Poplar Branches" by the Management Office of Lushan Nature Reserve in Jiangxi. Powder, α-naphthalene acetic acid, and 2, 4-D four plant growth regulators were used as the treatment solution before postharvest storage and transportation. After 24 hours of treatment, indolebutyric acid 200 mg/kg had the best effect, which could prolong the growth of poplar tree. The preservation life is up to 35 days.
发明内容 Contents of the invention
本发明要解决的技术问题是提供一种杨桐切枝保鲜预处理母液生产方法及应用技术。The technical problem to be solved by the present invention is to provide a production method and application technology of the pretreatment mother liquor for fresh-keeping and fresh-keeping of poplar tree branches.
解决该技术问题采用如下技术方案:To solve this technical problem, the following technical solutions are adopted:
本杨桐切枝保鲜预处理母液生产方法按下列步骤进行:The production method of the poplar cut branch fresh-keeping pretreatment mother liquor is carried out according to the following steps:
(1)备料:由市售获得80-320重量份蔗糖、0.08-0.32重量份抗坏血酸、0.16-0.20重量份乙酰水杨酸、0.08-0.12重量份噻苯隆和4000重量份水;(1) Material preparation: 80-320 parts by weight of sucrose, 0.08-0.32 parts by weight of ascorbic acid, 0.16-0.20 parts by weight of acetylsalicylic acid, 0.08-0.12 parts by weight of thiadizuron and 4000 parts by weight of water are obtained from the market;
(2)噻苯隆溶液的制备:用浓度为0.1 mol/L的NaOH溶液缓慢加入在0.08-0.12重量份噻苯隆中至全溶即止,再用浓度为0.1 mol/L HCL调节pH值为7,制成噻苯隆溶液;(2) Preparation of thiadizuron solution: Slowly add 0.08-0.12 parts by weight of thiadiuron into 0.08-0.12 parts by weight of NaOH solution with a concentration of 0.1 mol/L until it is completely dissolved, and then adjust the pH value with 0.1 mol/L HCL For 7, make Thidiazuron solution;
(3)混合成杨桐切枝保鲜预处理母液:将步骤(2)用0.08-0.12重量份噻苯隆制成的噻苯隆溶液、80-320重量份蔗糖、0.08-0.32重量份抗坏血酸、0.16-0.20重量份乙酰水杨酸和4000重量份水加入于混合缸中搅拌均匀,桶装即成。(3) Mixing into the pretreatment mother liquor of poplar tree branches fresh-keeping: thiadiuron solution prepared in step (2) with 0.08-0.12 parts by weight of thidiazuron, 80-320 parts by weight of sucrose, 0.08-0.32 parts by weight of ascorbic acid, 0.16-0.20 parts by weight of acetylsalicylic acid and 4000 parts by weight of water are added into a mixing tank, stirred evenly, and then packed in a barrel.
用上述杨桐切枝保鲜预处理母液生产方法制得的母液的应用按下列步骤进行:The application of the mother liquor prepared by the above-mentioned Yangtong cut branch fresh-keeping pretreatment mother liquor production method is carried out according to the following steps:
(1)母液的稀释:用母液与稀释水之重量比为1:4的比例稀释成杨桐切枝保鲜预处理液;(1) Dilution of the mother liquor: Dilute the mother liquor and the dilution water in a ratio of 1:4 to form a fresh-keeping pretreatment liquid for cut branches of Yangtong;
(2)浸枝:在采集到的众杨桐枝条的下端逐步进行斜切处理后,分别捆成束,杨桐切枝束的众切口浸于上述的预处理液中24 h;(2) Soaking branches: After the collected branches of Yangtong are gradually obliquely cut, they are bundled into bundles, and the incisions of the cut branches of Yangtong are immersed in the above-mentioned pretreatment solution for 24 hours;
(3)杨桐切枝束众切口的液棉包裹:将浸24 h后取出的杨桐,用浸润有杨桐切枝保鲜预处理液的脱脂棉,逐束包裹杨桐切枝束的众切口后,送至冷库低温储运,完成了杨桐切枝保鲜的预处理工作。(3) Wrapping with liquid cotton of cut branches of Yangtong: take out Yangtong after soaking for 24 hours, wrap the cuts of cut branches of Yangtong bundle by bundle with absorbent cotton soaked with the fresh-keeping pretreatment liquid of cut branches of Yangtong Afterwards, it was sent to the cold storage for low-temperature storage and transportation, and the pretreatment of poplar branches to keep fresh was completed.
本发明的有益效果是为大宗出口日本的杨桐切枝提供了一种切实有效的保鲜预处理母液,比用清水和用200 mg/kg吲哚丁酸保鲜液处理分别延长14天和10天的保鲜期,还比吲哚丁酸节省一半的成本,为解决储运期长的杨桐切枝保鲜难题开辟了新的途径;另一方面,以母液为成品,临时用临时稀释,降低储运与包装成本。The beneficial effect of the present invention is to provide a kind of practical and effective fresh-keeping pretreatment mother liquor for the poplar cut branches exported to Japan in large quantities, which is 14 days and 10 days longer than the treatment with clear water and 200 mg/kg indole butyric acid fresh-keeping solution respectively It also saves half the cost of indole butyric acid, which opens up a new way to solve the problem of keeping fresh poplar branches with long storage and transportation period; Shipping and packaging costs.
具体实施方式 Detailed ways
本发明下面结合实施例做进一步详述:先将各原料及其重量份配比按9项实施例列于表(1)。The present invention will be further described in detail below in conjunction with the examples: first, each raw material and its weight ratio are listed in Table (1) according to 9 examples.
实施例1(对照表(1)中实施例1的原料及重量分配比值):先将0.08份噻苯隆粉剂用浓度为0.1 mol/L NaOH至全溶,再用浓度为0.1 mol/L HCL调节pH为7(因NaOH与HCL浓度小、用量少,其重量可忽略不计),配制成噻苯隆溶液,再与80份蔗糖、0.08份抗坏血酸、0.16份乙酰水杨酸和4000份工业用水搅拌混匀,桶装即成。为节省成本,以上原料中蔗糖可用食品级,其它用工业纯,尽量少用分析纯。其余实施例2-9,均可对照表中相应实施例的原料及其重量份配比值,与实施例1相同方法制成。其中以实施例4优选。Example 1 (the raw materials and weight distribution ratios of Example 1 in the comparison table (1): first dissolve 0.08 parts of thiadizuron powder with a concentration of 0.1 mol/L NaOH to complete dissolution, and then use a concentration of 0.1 mol/L HCL Adjust the pH to 7 (the weight of NaOH and HCL is negligible due to the low concentration and dosage of NaOH and HCL), and prepare a thidiazuron solution, and then mix it with 80 parts of sucrose, 0.08 parts of ascorbic acid, 0.16 parts of acetylsalicylic acid and 4000 parts of industrial Stir with water to mix well, and serve in barrels. In order to save costs, the sucrose in the above raw materials can be used in food grade, and the others can be used in industrial grades, and analytical grades should be used as little as possible. All the rest of Examples 2-9 can be prepared in the same way as in Example 1 by comparing the raw materials and their weight ratios in the corresponding examples in the table. Wherein preferred with embodiment 4.
表(1)Table 1)
杨桐切枝在运输过程中常出现叶片黄化的问题,本方法中采用新型的高效植物生长调节剂噻苯隆(N-苯基-N-1,2,3-噻二唑-5-脲,英文通名thidiazuron,缩写为TDZ),它具有极强的生物活性,其诱导植物细胞分裂的能力比一般细胞分裂素高出1000倍以上。最初高浓度的噻苯隆在棉花收获上用作落叶剂使用,价格低廉,近些年发现低浓度对观赏植物有保鲜作用,因此它用于抑制杨桐叶片的黄化应该有一定效果。目前杨桐切枝只使用切口浸水后结合低温储运技术并不能解决叶片黄化的问题,保鲜效果尚欠理想。The yellowing of leaves often occurs during the transportation of poplar branches. In this method, a new type of high-efficiency plant growth regulator, thiadizuron (N-phenyl-N-1,2,3-thiadiazole-5-urea , English name thidiazuron, abbreviated as TDZ), it has extremely strong biological activity, and its ability to induce plant cell division is more than 1000 times higher than that of general cytokinins. Initially, high-concentration thiadiuron was used as a defoliant in cotton harvesting, and the price was low. In recent years, it has been found that low-concentration has a fresh-keeping effect on ornamental plants, so it should have a certain effect on inhibiting the yellowing of poplar leaves. At present, cutting branches of Yangtong only uses the incision soaked in water combined with low-temperature storage and transportation technology, which cannot solve the problem of leaf yellowing, and the fresh-keeping effect is not ideal.
原料中,蔗糖为切枝的枝叶细胞提供营养,调节细胞渗透压,促进水分吸收;抗坏血酸能降低保鲜液pH值,抑制微生物增殖,减少导管堵塞,另外还具有抗氧化作用,可显著减缓切枝蛋白含量的下降、维持细胞膜的完整性。乙酰水杨酸是杀菌剂,具有防止有害微生物繁殖和抑制乙烯生成的作用。噻苯隆是一种新型高效的植物生长调节剂,它可以抑制叶绿素的降解,通过对其它的植物激素和生理活性物质的作用来调节植物的生长发育过程,由于抑制叶片黄化的作用明显,比用清水和用200 mg/kg吲哚丁酸保鲜液处理,分别延缓杨桐叶片黄化达14天和10天。Among the raw materials, sucrose provides nutrition for the cut branches and leaf cells, regulates cell osmotic pressure, and promotes water absorption; ascorbic acid can reduce the pH value of the fresh-keeping solution, inhibit microbial proliferation, and reduce catheter blockage. Decrease in protein content and maintain the integrity of cell membrane. Acetylsalicylic acid is a fungicide, which has the effect of preventing the reproduction of harmful microorganisms and inhibiting the production of ethylene. Thidiazuron is a new type of high-efficiency plant growth regulator, it can inhibit the degradation of chlorophyll, regulate the growth and development of plants through the action of other plant hormones and physiologically active substances, due to the obvious effect of inhibiting leaf yellowing, Compared with water treatment and 200 mg/kg indole butyric acid preservative solution, the yellowing of Yangtong leaves was delayed for 14 days and 10 days, respectively.
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CN107926937A (en) * | 2017-11-22 | 2018-04-20 | 四川农业大学 | A kind of method for extending azalea branch fresh keeping time |
CN109122672A (en) * | 2018-07-18 | 2019-01-04 | 安徽德昌苗木有限公司 | A kind of storage method of oil tea breeding fringe bar heat preserving antistaling |
CN117571935A (en) * | 2024-01-15 | 2024-02-20 | 海南大学三亚南繁研究院 | Application of TDZ solution in-vitro identification of bacterial leaf blight resistance of rice and identification method |
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CN1486605A (en) * | 2003-08-05 | 2004-04-07 | 湖南农业大学 | Anthurium fresh-cut flower preservative |
CN1602688A (en) * | 2004-08-19 | 2005-04-06 | 中国农业大学 | Gerbera cut flower pretreatment solution and method of use thereof |
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CN1486605A (en) * | 2003-08-05 | 2004-04-07 | 湖南农业大学 | Anthurium fresh-cut flower preservative |
CN1602688A (en) * | 2004-08-19 | 2005-04-06 | 中国农业大学 | Gerbera cut flower pretreatment solution and method of use thereof |
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ANTONIO FERRANTE 等: "Effect of thidiazuron and gibberellic acid on leaf yellowing of cut stock flowers", 《CENTRAL EUROPEAN JOURNAL OF BIOLOGY》 * |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107926937A (en) * | 2017-11-22 | 2018-04-20 | 四川农业大学 | A kind of method for extending azalea branch fresh keeping time |
CN109122672A (en) * | 2018-07-18 | 2019-01-04 | 安徽德昌苗木有限公司 | A kind of storage method of oil tea breeding fringe bar heat preserving antistaling |
CN117571935A (en) * | 2024-01-15 | 2024-02-20 | 海南大学三亚南繁研究院 | Application of TDZ solution in-vitro identification of bacterial leaf blight resistance of rice and identification method |
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