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BRPI0401650A - Extração rápida de rna de células e tecidos - Google Patents

Extração rápida de rna de células e tecidos

Info

Publication number
BRPI0401650A
BRPI0401650A BR0401650-5A BRPI0401650A BRPI0401650A BR PI0401650 A BRPI0401650 A BR PI0401650A BR PI0401650 A BRPI0401650 A BR PI0401650A BR PI0401650 A BRPI0401650 A BR PI0401650A
Authority
BR
Brazil
Prior art keywords
rna
tissues
cells
substrate
rna extraction
Prior art date
Application number
BR0401650-5A
Other languages
English (en)
Inventor
Robert Belly
Jacqueline Toner
John Backus
Original Assignee
Johnson & Johnson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johnson & Johnson filed Critical Johnson & Johnson
Publication of BRPI0401650A publication Critical patent/BRPI0401650A/pt

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B42—BOOKBINDING; ALBUMS; FILES; SPECIAL PRINTED MATTER
    • B42D—BOOKS; BOOK COVERS; LOOSE LEAVES; PRINTED MATTER CHARACTERISED BY IDENTIFICATION OR SECURITY FEATURES; PRINTED MATTER OF SPECIAL FORMAT OR STYLE NOT OTHERWISE PROVIDED FOR; DEVICES FOR USE THEREWITH AND NOT OTHERWISE PROVIDED FOR; MOVABLE-STRIP WRITING OR READING APPARATUS
    • B42D5/00—Sheets united without binding to form pads or blocks
    • B42D5/04—Calendar blocks
    • B42D5/043—Supports for desk-type calendars or diaries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • G—PHYSICS
    • G09—EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
    • G09F—DISPLAYING; ADVERTISING; SIGNS; LABELS OR NAME-PLATES; SEALS
    • G09F23/00—Advertising on or in specific articles, e.g. ashtrays, letter-boxes
    • G09F23/10—Advertising on or in specific articles, e.g. ashtrays, letter-boxes on paper articles, e.g. booklets, newspapers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Theoretical Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Extraction Or Liquid Replacement (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

"EXTRAçãO RáPIDA DE RNA DE CéLULAS E TECIDOS". A presente invenção refere-se a um método de extrair RNA de sistemas biológicos (células, fragmentos celulares, organelas, tecidos, órgãos ou organismos) no qual uma solução contendo RNA é contatada com um substrato ao qual o RNA pode ligar-se. O RNA é retirado do substrato aplicando-se pressão negativa. Nenhuma etapa de centrifugação excede a trinta segundos. Preferivelmente, o RNA é diluído antes da filtragem e uma ou mais etapas de lavagem podem ser empregadas para remover interferentes. Em um tal método, exceto para cisalhamento de DNA e etapas de secagem, a centrifugação não é empreendida durante a etapa de extração.
BR0401650-5A 2003-05-01 2004-05-03 Extração rápida de rna de células e tecidos BRPI0401650A (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US10/427,217 US7267950B2 (en) 2003-05-01 2003-05-01 Rapid extraction of RNA from cells and tissues

Publications (1)

Publication Number Publication Date
BRPI0401650A true BRPI0401650A (pt) 2005-07-12

Family

ID=33310080

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0401650-5A BRPI0401650A (pt) 2003-05-01 2004-05-03 Extração rápida de rna de células e tecidos

Country Status (11)

Country Link
US (1) US7267950B2 (pt)
EP (1) EP1559784A3 (pt)
JP (3) JP2004329213A (pt)
KR (1) KR20040094649A (pt)
CN (1) CN100471861C (pt)
AR (1) AR044159A1 (pt)
AU (1) AU2004201841A1 (pt)
BR (1) BRPI0401650A (pt)
CA (1) CA2463716A1 (pt)
HK (1) HK1080483A1 (pt)
MX (1) MXPA04004243A (pt)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT1757705E (pt) 2003-05-01 2009-02-25 Veridex Llc Ensaio intra-operatório de nódulo linfático
DE10358137A1 (de) * 2003-12-12 2005-07-07 Merck Patent Gmbh Verfahren und Kit zur Isolierung von RNA
BRPI0510645A (pt) * 2004-06-04 2007-11-20 Veridex Llc diagnóstico ou prognóstico do progresso do cáncer de mama, composição compreendendo conjuntos de iniciador/sonda de ácido nucléico e kit para análise de linfonodo intraoperativo
KR100843168B1 (ko) 2004-12-10 2008-07-02 주식회사 엘지생활건강 올리고뉴클레오타이드를 포함하는 미백제
EP1915461B1 (en) * 2005-08-17 2018-08-01 Dx4U GmbH Composition and method for determination of ck19 expression
US20070281305A1 (en) * 2006-06-05 2007-12-06 Sean Wuxiong Cao Detection of lymph node metastasis from gastric carcinoma
US8389215B2 (en) 2007-07-26 2013-03-05 University Of Utah Research Foundation Non-invasive recovery of RNA and analysis of gene expression in skin
DE102007035250A1 (de) * 2007-07-27 2009-01-29 Qiagen Gmbh Verfahren zum Abtrennen von nicht-proteinhaltigen Biomolekülen, insbesondere Nukleinsäuren aus proteinhaltigen Proben
GB2463401B (en) 2008-11-12 2014-01-29 Caris Life Sciences Luxembourg Holdings S A R L Characterizing prostate disorders by analysis of microvesicles
US8790916B2 (en) 2009-05-14 2014-07-29 Genestream, Inc. Microfluidic method and system for isolating particles from biological fluid
EP2507393A4 (en) 2009-11-30 2013-05-01 Caris Life Sciences Luxembourg Holdings METHODS AND SYSTEMS FOR ISOLATING, STORING AND ANALYZING VESICLES
CA2787027A1 (en) 2010-01-13 2011-07-21 Caris Life Sciences Luxembourg Holdings, S.A.R.L. Detection of gastrointestinal disorders
JP5808349B2 (ja) 2010-03-01 2015-11-10 カリス ライフ サイエンシズ スウィッツァーランド ホールディングスゲーエムベーハー セラノーシスのためのバイオマーカー
EP2556172A4 (en) 2010-04-06 2013-10-30 Caris Life Sciences Luxembourg Holdings CIRCULATING BIOMARKERS FOR DISEASES
US9046507B2 (en) 2010-07-29 2015-06-02 Gen-Probe Incorporated Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure
US9040679B2 (en) 2012-04-30 2015-05-26 General Electric Company Methods and compositions for extraction and storage of nucleic acids
US9040675B2 (en) 2012-04-30 2015-05-26 General Electric Company Formulations for nucleic acid stabilization on solid substrates
US9044738B2 (en) * 2012-04-30 2015-06-02 General Electric Company Methods and compositions for extraction and storage of nucleic acids
US9480966B2 (en) 2012-04-30 2016-11-01 General Electric Company Substrates and methods for collection, stabilization and elution of biomolecules
CN102864139A (zh) * 2012-09-11 2013-01-09 西安交通大学口腔医院 一种用于组织细胞基因组dna的核酸吸附快速分离方法
CN105431536B (zh) * 2013-08-16 2021-01-01 通用电气公司 用于提取和储存核酸的方法和组合物
EP3636742A1 (en) * 2014-04-25 2020-04-15 Translate Bio, Inc. Methods for purification of messenger rna
FI4035762T3 (fi) 2015-09-09 2023-12-04 Drawbridge Health Inc Laitteita näytteiden keräämistä, stabilointia ja säilytystä varten
CA3054321A1 (en) * 2017-02-27 2018-08-30 Translate Bio, Inc. Methods for purification of messenger rna

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3389133A (en) 1966-08-10 1968-06-18 Schwarz Biores Inc Process for isolating and purifying soluble ribonucleic acid
US4843155A (en) 1987-11-19 1989-06-27 Piotr Chomczynski Product and process for isolating RNA
US5010183A (en) 1989-07-07 1991-04-23 Macfarlane Donald E Process for purifying DNA and RNA using cationic detergents
DE3935098C2 (de) * 1989-10-21 1995-05-24 Macherey Nagel & Co Chem Chromatographisches Trägermaterial sowie seine Verwendung in einem Verfahren zur chromatographischen Trennung von Nukleinsäuren
US5654179A (en) 1990-11-14 1997-08-05 Hri Research, Inc. Nucleic acid preparation methods
US5620852A (en) 1990-11-14 1997-04-15 Hri Research, Inc. Nucleic acid preparation methods
US5155018A (en) 1991-07-10 1992-10-13 Hahnemann University Process and kit for isolating and purifying RNA from biological sources
US5346994A (en) 1992-01-28 1994-09-13 Piotr Chomczynski Shelf-stable product and process for isolating RNA, DNA and proteins
US5438128A (en) 1992-02-07 1995-08-01 Millipore Corporation Method for rapid purifiction of nucleic acids using layered ion-exchange membranes
KR100230909B1 (ko) 1993-11-29 1999-12-01 다니엘 엘. 캐시앙 광범위의 유기체로부터 핵산 추출 방법
EP0743950B1 (de) 1994-02-11 2001-12-12 QIAGEN GmbH Verfahren zur trennung von doppelstrang/einzelstrangnukleinsäurestrukturen
DE69528256T2 (de) 1994-04-14 2003-07-31 The Rockefeller University, New York Vorrichtung zur isolierung von zellularinhaltsstoffen
US5643767A (en) 1994-05-02 1997-07-01 The Rockefeller University Process for isolating cellular components
JP3007161B2 (ja) * 1994-12-01 2000-02-07 オクラホマ メディカル リサーチ ファウンデーション コレステロール吸収を減少するための方法および組成物
US5777099A (en) 1995-02-24 1998-07-07 Biotecx Laboratories, Inc. RNA separation
US5945515A (en) 1995-07-31 1999-08-31 Chomczynski; Piotr Product and process for isolating DNA, RNA and proteins
US5973137A (en) 1996-02-13 1999-10-26 Gentra Systems, Inc. Low pH RNA isolation reagents, method, and kit
DE19702907A1 (de) * 1997-01-28 1998-07-30 Boehringer Mannheim Gmbh Verfahren und Vorrichtung zur Reinigung von Nukleinsäuren
JP2001524820A (ja) 1997-04-10 2001-12-04 ライフ テクノロジーズ，インコーポレイテッド Ｒｎａ単離試薬および方法
US6218531B1 (en) 1997-06-25 2001-04-17 Promega Corporation Method of isolating RNA
DE19746874A1 (de) * 1997-10-23 1999-04-29 Qiagen Gmbh Verfahren zur Isolierung und Reinigung von Nukleinsäuren an hydrophoben Oberflächen - insbesondere unter Verwendung hydrophober Membranen
CN1220995A (zh) * 1997-12-23 1999-06-30 陈铁骅 总rna提取试剂及其制造方法以及提取总rna的方法
US6204375B1 (en) 1998-07-31 2001-03-20 Ambion, Inc. Methods and reagents for preserving RNA in cell and tissue samples
JP2000166556A (ja) * 1998-12-10 2000-06-20 Hitachi Ltd 核酸の回収方法及び装置
CA2299119C (en) * 1999-02-23 2013-02-05 Qiagen Gmbh A method of stabilizing and/or isolating nucleic acids
JP2001037491A (ja) * 1999-05-26 2001-02-13 Hayashibara Biochem Lab Inc トレハラーゼをコードするｄｎａとその用途
US6248535B1 (en) 1999-12-20 2001-06-19 University Of Southern California Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens
ATE353974T1 (de) 2000-04-03 2007-03-15 Corixa Corp Verfahren, zusammensetzungen und kits zur identifizierung und überwachung von brustkrebs
US7033801B2 (en) * 2000-12-08 2006-04-25 Invitrogen Corporation Compositions and methods for rapidly generating recombinant nucleic acid molecules
AU2002227153B2 (en) * 2000-12-08 2008-01-24 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
DE60210963T2 (de) 2001-01-16 2006-11-23 Invitrogen Corp., Carlsbad Reagenz zur isolierung von rna
EP1418943A1 (en) * 2001-02-14 2004-05-19 Protein Design Labs, Inc. Methods of diagnosis of angiogenesis, compositions and methods of screening for angiogenesis modulators
US7101663B2 (en) 2001-03-02 2006-09-05 University of Pittsburgh—of the Commonwealth System of Higher Education PCR method

Also Published As

Publication number Publication date
MXPA04004243A (es) 2004-11-29
AU2004201841A1 (en) 2004-11-18
CA2463716A1 (en) 2004-11-01
US20040219534A1 (en) 2004-11-04
CN100471861C (zh) 2009-03-25
EP1559784A2 (en) 2005-08-03
AR044159A1 (es) 2005-08-24
US7267950B2 (en) 2007-09-11
JP2004329213A (ja) 2004-11-25
HK1080483A1 (en) 2006-04-28
JP2014132905A (ja) 2014-07-24
EP1559784A3 (en) 2005-08-31
KR20040094649A (ko) 2004-11-10
JP2011115173A (ja) 2011-06-16
CN1680422A (zh) 2005-10-12

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B11E Dismissal acc. art. 34 of ipl - requirements for examination incomplete
B11T Dismissal: dismissal of application maintained