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BR112017005354A2 - métodos de criação de um banco de células de densidade ultra-alta - Google Patents

métodos de criação de um banco de células de densidade ultra-alta

Info

Publication number
BR112017005354A2
BR112017005354A2 BR112017005354A BR112017005354A BR112017005354A2 BR 112017005354 A2 BR112017005354 A2 BR 112017005354A2 BR 112017005354 A BR112017005354 A BR 112017005354A BR 112017005354 A BR112017005354 A BR 112017005354A BR 112017005354 A2 BR112017005354 A2 BR 112017005354A2
Authority
BR
Brazil
Prior art keywords
ultra
high density
methods
creating
cell
Prior art date
Application number
BR112017005354A
Other languages
English (en)
Other versions
BR112017005354B1 (pt
Inventor
Buser Claudia
Jin Xiaoxia
Original Assignee
Genzyme Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genzyme Corp filed Critical Genzyme Corp
Publication of BR112017005354A2 publication Critical patent/BR112017005354A2/pt
Publication of BR112017005354B1 publication Critical patent/BR112017005354B1/pt

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/12—Chemical aspects of preservation
    • A01N1/122—Preservation or perfusion media
    • A01N1/125—Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/14—Mechanical aspects of preservation; Apparatus or containers therefor
    • A01N1/142—Apparatus
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/16—Physical preservation processes
    • A01N1/162—Temperature processes, e.g. following predefined temperature changes over time
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/26—Constructional details, e.g. recesses, hinges flexible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C12M3/06—Tissue, human, animal or plant cell, or virus culture apparatus with filtration, ultrafiltration, inverse osmosis or dialysis means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/14—Bags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/16—Vibrating; Shaking; Tilting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Sustainable Development (AREA)
  • Clinical Laboratory Science (AREA)
  • Cell Biology (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Virology (AREA)
  • Water Supply & Treatment (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Analytical Chemistry (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)

Abstract

são fornecidos métodos para a criação de bancos de células criopreservadas de densidade ultra-alta. em certas modalidades, estes métodos empregam técnicas de cultura de perfusão alteradas que levam em conta a produção de culturas de células de densidade ultra-alta que podem ser criopreservadas em densidades de células inesperadamente elevadas sem a necessidade de quaisquer etapas de concentração celular, enquanto retém uma excelente viabilidade e qualidade celular.
BR112017005354-3A 2014-09-18 2015-09-17 Métodos de criação de um banco de células de densidade ultra-alta BR112017005354B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462052257P 2014-09-18 2014-09-18
US62/052,257 2014-09-18
PCT/US2015/050817 WO2016044670A1 (en) 2014-09-18 2015-09-17 Ultra-high density cell banking methods

Publications (2)

Publication Number Publication Date
BR112017005354A2 true BR112017005354A2 (pt) 2017-12-12
BR112017005354B1 BR112017005354B1 (pt) 2021-06-22

Family

ID=54249621

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017005354-3A BR112017005354B1 (pt) 2014-09-18 2015-09-17 Métodos de criação de um banco de células de densidade ultra-alta

Country Status (18)

Country Link
US (2) US20160100570A1 (pt)
EP (2) EP3193594B1 (pt)
JP (4) JP6760927B2 (pt)
KR (3) KR20240005197A (pt)
CN (2) CN117958251A (pt)
AU (4) AU2015317530A1 (pt)
BR (1) BR112017005354B1 (pt)
CA (1) CA2962262A1 (pt)
CY (1) CY1124672T1 (pt)
DK (1) DK3193594T3 (pt)
ES (1) ES2890454T3 (pt)
HU (1) HUE055676T2 (pt)
IL (5) IL303768B2 (pt)
MX (2) MX383858B (pt)
RU (1) RU2723129C2 (pt)
SG (2) SG11201702087QA (pt)
TW (4) TWI781387B (pt)
WO (1) WO2016044670A1 (pt)

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JP3022963B2 (ja) 1998-04-20 2000-03-21 東京ラヂエーター製造株式会社 Ｅｇｒ熱交換装置
CN112410279A (zh) 2013-03-15 2021-02-26 建新公司 高密度细胞存储方法
KR101813048B1 (ko) * 2014-10-30 2017-12-29 린나이코리아 주식회사 플레이트 접합형 열교환기
EP3442688A4 (en) 2016-04-12 2019-11-20 Artemis Biosystems, Inc. FILTRATION SYSTEMS FOR INFUSION
JP6870629B2 (ja) * 2018-02-09 2021-05-12 Ｊｆｅエンジニアリング株式会社 バイオリアクターバッグのスケールアップ方法
JP7333930B2 (ja) * 2019-01-07 2023-08-28 独立行政法人国立高等専門学校機構 ミミズ細胞の保存方法及びミミズ培養細胞の形質転換方法
CN109892321B (zh) * 2019-04-17 2025-03-28 中国科学技术大学 一种大体积生物样本低温保存的方法
CN110628700B (zh) * 2019-10-14 2023-10-17 广东省中医院（广州中医药大学第二附属医院、广州中医药大学第二临床医学院、广东省中医药科学院） 一种用于筛选细胞培养条件的标准方法
AU2021324669A1 (en) * 2020-08-10 2023-02-23 Angiocrine Bioscience, Inc. Cryopreserved endothelial cell compositions
CN116179464A (zh) * 2022-12-06 2023-05-30 无锡药明生物技术股份有限公司 提高培养液细胞密度和高密度细胞培养的方法
CN115644172A (zh) * 2022-12-27 2023-01-31 江苏赛孚士生物技术有限公司 细胞冻存方法和细胞复苏方法

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AUPN442295A0 (en) 1995-07-26 1995-08-17 Commonwealth Scientific And Industrial Research Organisation Regulated autocrine growth of mammalian cells
US6475725B1 (en) 1997-06-20 2002-11-05 Baxter Aktiengesellschaft Recombinant cell clones having increased stability and methods of making and using the same
AT407255B (de) 1997-06-20 2001-02-26 Immuno Ag Rekombinanter zellklon mit erhöhter stabilität in serum- und proteinfreiem medium und verfahren zur gewinnung des stabilen zellklons
US6391257B1 (en) 1998-08-19 2002-05-21 Prestone Products Corporation Antifreeze compositions comprising carboxylic acid and cyclohexenoic acid
EP1103610A1 (en) 1999-11-26 2001-05-30 Introgene B.V. Production of vaccines from immortalised mammalian cell lines
US6544424B1 (en) 1999-12-03 2003-04-08 Refined Technology Company Fluid filtration system
US6544788B2 (en) 2001-02-15 2003-04-08 Vijay Singh Disposable perfusion bioreactor for cell culture
JP2007525984A (ja) * 2004-03-05 2007-09-13 ディーエスエム アイピー アセッツ ビー．ブイ． 連続的灌流および交互接線流による細胞培養の方法
RU2290808C2 (ru) * 2004-12-06 2007-01-10 Институт физиологии Коми научного центра Уральского отделения Российской Академии Наук Криопротекторный раствор для замораживания лейкоцитов при низкой температуре
RU2314687C1 (ru) * 2006-07-13 2008-01-20 Институт биологии моря имени А.В. Жирмунского Дальневосточного отделения Российской академии наук (статус государственного учреждения) Способ криосохранения клеток морских беспозвоночных
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CN112410279A (zh) * 2013-03-15 2021-02-26 建新公司 高密度细胞存储方法

Also Published As

Publication number Publication date
ES2890454T3 (es) 2022-01-19
TW202328427A (zh) 2023-07-16
JP7487263B2 (ja) 2024-05-20
CY1124672T1 (el) 2022-07-22
AU2024220017A1 (en) 2024-10-17
AU2019261787A1 (en) 2019-11-28
SG10201902355VA (en) 2019-04-29
US20160100570A1 (en) 2016-04-14
CN117958251A (zh) 2024-05-03
TWI781387B (zh) 2022-10-21
EP3957173A1 (en) 2022-02-23
HUE055676T2 (hu) 2021-12-28
AU2022204128B2 (en) 2024-07-11
IL303768B1 (en) 2024-05-01
DK3193594T3 (da) 2021-09-27
RU2723129C2 (ru) 2020-06-08
IL274458B (en) 2021-12-01
MX2017003651A (es) 2018-01-24
IL274458A (en) 2020-06-30
SG11201702087QA (en) 2017-04-27
US20230309551A1 (en) 2023-10-05
KR20230020014A (ko) 2023-02-09
JP6760927B2 (ja) 2020-09-23
JP2017534581A (ja) 2017-11-24
CA2962262A1 (en) 2016-03-24
JP2020198889A (ja) 2020-12-17
TW202102659A (zh) 2021-01-16
AU2019261787B2 (en) 2022-03-17
JP2024105417A (ja) 2024-08-06
RU2017113076A (ru) 2018-10-18
EP3193594B1 (en) 2021-07-07
CN107426990A (zh) 2017-12-01
IL303768B2 (en) 2024-09-01
IL251206B (en) 2020-05-31
MX383858B (es) 2025-03-14
IL303768A (en) 2023-08-01
TW201614063A (en) 2016-04-16
BR112017005354B1 (pt) 2021-06-22
MX2021007613A (es) 2021-08-11
EP3193594A1 (en) 2017-07-26
WO2016044670A1 (en) 2016-03-24
KR102619142B1 (ko) 2023-12-29
TWI844120B (zh) 2024-06-01
JP7104116B2 (ja) 2022-07-20
IL251206A0 (en) 2017-05-29
IL311946A (en) 2024-06-01
IL287921A (en) 2022-01-01
AU2022204128A1 (en) 2022-06-30
IL287921B2 (en) 2023-11-01
TWI709646B (zh) 2020-11-11
JP2022133397A (ja) 2022-09-13
RU2017113076A3 (pt) 2019-03-11
IL287921B1 (en) 2023-07-01
TW202500741A (zh) 2025-01-01
KR20240005197A (ko) 2024-01-11
RU2020116448A (ru) 2020-07-31
AU2015317530A1 (en) 2017-04-13
KR20170054507A (ko) 2017-05-17

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Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 17/09/2015, OBSERVADAS AS CONDICOES LEGAIS.