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MX2020009670A - Metodos de cultivo por perfusion y usos de los mismos. - Google Patents

Metodos de cultivo por perfusion y usos de los mismos.

Info

Publication number
MX2020009670A
MX2020009670A MX2020009670A MX2020009670A MX2020009670A MX 2020009670 A MX2020009670 A MX 2020009670A MX 2020009670 A MX2020009670 A MX 2020009670A MX 2020009670 A MX2020009670 A MX 2020009670A MX 2020009670 A MX2020009670 A MX 2020009670A
Authority
MX
Mexico
Prior art keywords
methods
culturing methods
perfusion culturing
culturing
perfusion
Prior art date
Application number
MX2020009670A
Other languages
English (en)
Inventor
Jianguo Yang
Yang Yang
Agata Villiger-Oberbek
Gabrielle Lorenzo
Original Assignee
Genzyme Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genzyme Corp filed Critical Genzyme Corp
Publication of MX2020009670A publication Critical patent/MX2020009670A/es

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • C12M1/26Inoculator or sampler
    • C12M1/32Inoculator or sampler multiple field or continuous type
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/045Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/12Well or multiwell plates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/10Rotating vessel
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10Perfusion
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00Genetically modified cells
    • C12N2510/02Cells for production
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2527/00Culture process characterised by the use of mechanical forces, e.g. strain, vibration

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

En la presente descripción se proporcionan métodos para cultivar una célula de mamífero y varios métodos que utilizan estos métodos de cultivo. Asimismo, se proporcionan placas de cultivo celular de múltiples pocillos, por ejemplo, para uso en métodos de cultivo por perfusión.
MX2020009670A 2014-06-06 2016-12-06 Metodos de cultivo por perfusion y usos de los mismos. MX2020009670A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201462009058P 2014-06-06 2014-06-06

Publications (1)

Publication Number Publication Date
MX2020009670A true MX2020009670A (es) 2020-10-08

Family

ID=53484156

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2016016142A MX2016016142A (es) 2014-06-06 2015-06-05 Metodos de cultivo por perfusion y usos de los mismos.
MX2020009670A MX2020009670A (es) 2014-06-06 2016-12-06 Metodos de cultivo por perfusion y usos de los mismos.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2016016142A MX2016016142A (es) 2014-06-06 2015-06-05 Metodos de cultivo por perfusion y usos de los mismos.

Country Status (14)

Country Link
US (3) US11060058B2 (es)
EP (1) EP3152293A2 (es)
JP (3) JP6915992B2 (es)
KR (2) KR102611855B1 (es)
CN (1) CN106795495A (es)
AU (2) AU2015269184B2 (es)
BR (1) BR112016028512A8 (es)
CA (1) CA2951255A1 (es)
IL (1) IL249384A0 (es)
MX (2) MX2016016142A (es)
RU (3) RU2708919C2 (es)
SG (2) SG11201610167WA (es)
TW (2) TWI743024B (es)
WO (1) WO2015188106A2 (es)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2901950A1 (en) 2013-02-22 2014-08-28 Genzyme Corporation Microcarrier perfusion culturing methods and uses thereof
WO2014130864A2 (en) 2013-02-22 2014-08-28 Genzyme Corporation Microcarrier perfusion culturing methods and uses thereof
TWI743024B (zh) * 2014-06-06 2021-10-21 美商健臻公司 灌注培養方法及其用途
TWI776235B (zh) * 2014-06-09 2022-09-01 美商健臻公司 種子罐培養法(seed train processes)及其用途
CA2971861C (en) 2014-12-22 2024-02-13 Genzyme Corporation Methods of culturing a mammalian cell
US11292999B2 (en) 2016-08-30 2022-04-05 Finesse Solutions, Inc. Bioreactor with multiple coupled vessels
EP3875595A4 (en) * 2018-11-02 2022-07-13 Kyowa Kirin Co., Ltd. Liquid medium preparation method
WO2022173964A1 (en) * 2021-02-10 2022-08-18 Koniku Inc. Assay system, methods, and multi-well plate for gas stimulation of biological cells, proteins or materials
MX2023012193A (es) * 2021-04-14 2023-10-25 Genzyme Corp Metodos de cultivo de perfusion de una celula de mamifero.
JP2023058900A (ja) * 2021-10-14 2023-04-26 株式会社三共 遊技機
JP2023058901A (ja) * 2021-10-14 2023-04-26 株式会社三共 遊技機
JP2023058899A (ja) * 2021-10-14 2023-04-26 株式会社三共 遊技機
JP2023058903A (ja) * 2021-10-14 2023-04-26 株式会社三共 遊技機
JP2023058902A (ja) * 2021-10-14 2023-04-26 株式会社三共 遊技機
WO2024112917A1 (en) * 2022-11-22 2024-05-30 The University Of North Carolina At Chapel Hill Laminar flow incorporated scaffold-based 3d cell cultures
DE102022214275A1 (de) * 2022-12-22 2024-06-27 Robert Bosch Gesellschaft mit beschränkter Haftung Vorrichtung und Verfahren zur Kultivierung von Zellen
US20240263122A1 (en) * 2023-02-08 2024-08-08 National Central University Bioreactor apparatus, ion-exchange substrate and method for regulation of ion concentration and cell culture thereof

Family Cites Families (73)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4522811A (en) 1982-07-08 1985-06-11 Syntex (U.S.A.) Inc. Serial injection of muramyldipeptides and liposomes enhances the anti-infective activity of muramyldipeptides
RU2058992C1 (ru) 1993-07-14 1996-04-27 Антипин Георгий Васильевич Способ получения кормового белка и устройство для его осуществления
DE19612966B4 (de) 1996-04-01 2009-12-10 Novartis Vaccines And Diagnostics Gmbh & Co. Kg MDCK-Zellen und Verfahren zur Vermehrung von Influenzaviren
RU2215748C2 (ru) 1999-04-09 2003-11-10 Сентро Де Инмунология Молекулар Способ получения рекомбинантного эритропоэтина человека и полученный эритропоэтин, фармацевтическая композиция
DK1161548T4 (da) 1999-04-15 2010-03-01 Crucell Holland Bv Rekombinant proteinproduktion i en human celle under anvendelse af sekvenser, der koder for adenovirus E1-protein
AT409379B (de) * 1999-06-02 2002-07-25 Baxter Ag Medium zur protein- und serumfreien kultivierung von zellen
UA74557C2 (en) 1999-09-03 2006-01-16 Applied Research Systems A method for producing a heterologous secreted protein from chinese hamster ovaries cells grown on microcarriers
US6585971B1 (en) 1999-11-12 2003-07-01 Harbor-Ucla Research And Education Institute Recombinant α-L-iduronidase, methods for producing and purifying the same and methods for treating disease caused by deficiencies thereof
ES2276853T3 (es) 2000-12-20 2007-07-01 Bayer Pharmaceuticals Corporation Dispositivo y procedimiento para expansion en serie de semillas de celulas de mamifero.
US6544788B2 (en) 2001-02-15 2003-04-08 Vijay Singh Disposable perfusion bioreactor for cell culture
JP2005509406A (ja) 2001-05-09 2005-04-14 バイエル・フアーマシユーチカルズ・コーポレーシヨン 哺乳動物細胞株のための低温保存袋アセンブリー
CN100443581C (zh) 2001-08-31 2008-12-17 拜耳医药保健股份公司 实现高细胞密度发酵的装置和方法
BR0212780A (pt) 2001-10-02 2004-10-13 Novo Nordisk Healthcare Ag Métodos para produzir polipeptìdeos em células eucarióticas, para cultivar células eucarióticas e para colher polipeptìdeos produzidos por células eucarióticas
EP1434953A1 (de) 2001-10-11 2004-07-07 Siemens Aktiengesellschaft Dämpfungsvorrichtung sowie verfahren zum bedämpfen von bewegungen in einer dämpfungsvorrichtung
WO2003039459A2 (en) 2001-11-05 2003-05-15 Genvec, Inc. Viral vector production methods and compositions
US20030091976A1 (en) 2001-11-14 2003-05-15 Ciphergen Biosystems, Inc. Methods for monitoring polypeptide production and purification using surface enhanced laser desorption/ionization mass spectrometry
US6953692B2 (en) 2001-12-18 2005-10-11 Bayer Pharmaceuticals Corporation Device and method for seed-train expansion of mammalian cells
US20050019914A1 (en) 2003-07-24 2005-01-27 Aventis Pharma Deutschland Gmbh Perfusion process for producing erythropoietin
AR046510A1 (es) 2003-07-25 2005-12-14 Regeneron Pharma Composicion de un antagonista de vegf y un agente anti-proliferativo
EP1508576A1 (en) 2003-08-20 2005-02-23 Crucell Holland B.V. Efficient production of IgA in recombinant mammalian cells
GB2411178B8 (en) 2004-02-20 2008-07-11 Cesco Bioengineering Co Ltd Apparatus and method for preparing and culturing cells
CN101061213B (zh) * 2004-05-19 2012-12-19 麻省理工学院 灌注的三维细胞/组织疾病模型
US7767446B2 (en) * 2004-09-16 2010-08-03 Becton, Dickinson And Company Perfusion bioreactors for culturing cells
AU2005287162B2 (en) * 2004-09-16 2010-12-23 Becton, Dickinson And Company Perfusion bioreactor for culturing cells
HUE027136T2 (en) 2004-09-30 2016-08-29 Bayer Healthcare Llc Tools and procedures for the integrated, continuous production of biological molecules
WO2006138143A1 (en) 2005-06-15 2006-12-28 Amprotein Corporation Suspension culture vessels
ES2662589T3 (es) 2005-06-15 2018-04-09 Zhejiang Jinyishengshi Bioengineering Co., Ltd. Recipientes para cultivo en suspensión
WO2008024769A2 (en) 2006-08-21 2008-02-28 Raven Biotechnologies, Inc. Intensified perfusion production method
WO2008073620A2 (en) 2006-11-02 2008-06-19 Neose Technologies, Inc. Manufacturing process for the production of polypeptides expressed in insect cell-lines
WO2008085962A2 (en) 2007-01-08 2008-07-17 Millipore Corporation Cell culture methods for producing recombinant proteins in the presence of reduced levels of one or more contaminants
WO2008106515A1 (en) * 2007-02-27 2008-09-04 The Board Of Trustees Of The University Of Illinois Insert device for multiwell plate
PT2126093E (pt) 2007-03-02 2012-12-03 Boehringer Ingelheim Pharma Melhoramento da produção de proteínas
EP2155347B1 (en) 2007-04-17 2013-07-17 Xendo Holding B.V. Method and device for continuous membrane adsorption
TW200902708A (en) 2007-04-23 2009-01-16 Wyeth Corp Methods of protein production using anti-senescence compounds
US9109193B2 (en) 2007-07-30 2015-08-18 Ge Healthcare Bio-Sciences Corp. Continuous perfusion bioreactor system
ES2657055T3 (es) 2007-08-09 2018-03-01 Wyeth Llc Uso de perfusión para mejorar la producción de un cultivo de células alimentado por lotes en biorreactores
WO2009034186A2 (en) 2007-09-13 2009-03-19 Helmholtz-Zentrum für Infektionsforschung GmbH Process for cell cultivation
US20090233334A1 (en) 2008-03-11 2009-09-17 Excellgene Sa Cell cultivation and production of recombinant proteins by means of an orbital shake bioreactor system with disposable bags at the 1,500 liter scale
DE102008019691A1 (de) 2008-04-15 2009-10-29 Technische Universität Ilmenau Teilaktives mikrofluidisches System für die 3D-Zellkultivierung sowie Verfahren zu dessen Perfusion
US20100076380A1 (en) 2008-06-16 2010-03-25 Amprotein Corporation Bioreactors
JP2012506257A (ja) * 2008-10-22 2012-03-15 バイオベスト インターナショナル インコーポレイテッド 細胞および細胞由来生成物の産生のための、灌流バイオリアクター、細胞培養システム、および方法
CN102449135B (zh) * 2009-03-26 2014-07-09 新加坡科技研究局 用于细胞或组织培养的设备
US8728479B2 (en) 2009-03-31 2014-05-20 The Trustees Of The University Of Pennsylvania Antigen-binding proteins comprising recombinant protein scaffolds
CN101875917B (zh) 2009-04-28 2012-07-04 北京清大天一科技有限公司 生物反应器微载体培养动物细胞生产猪瘟疫苗的方法
CA3118479A1 (en) 2009-07-06 2011-01-13 Genentech, Inc. Method of culturing eukaryotic cells
CA2791565C (fr) * 2010-03-02 2018-08-28 Universite Technologie De Compiegne - Utc Boite multi-reacteurs pour culture cellulaire dynamique
CN201933090U (zh) 2010-11-22 2011-08-17 朱学仁 细胞微载体灌注培养截留装置
JP2013544524A (ja) 2010-12-06 2013-12-19 ターポン バイオシステムズ,インコーポレイテッド 生物学的生成物の連続プロセス法
SG191745A1 (en) 2011-01-17 2013-08-30 Hoffmann La Roche Separation apparatus and use thereof
JP5709976B2 (ja) * 2011-03-22 2015-04-30 株式会社東海ヒット 顕微鏡観察用培養装置およびその使用方法
DK2707488T3 (en) 2011-05-12 2017-12-11 Probiogen Ag IMPROVEMENT OF PROTEIN PREPARATION YIELD MEDIATED BY CDC42 GTPASE ON FAST CYCLES
CA2952347A1 (en) 2011-07-01 2013-01-10 Amgen Inc. Mammalian cell culture
JP5703449B2 (ja) * 2011-07-15 2015-04-22 パナソニックIpマネジメント株式会社 携帯端末
DK2620561T3 (en) 2012-01-30 2015-02-16 Dannrup Neils Device and method for filtering surface
US20150004686A1 (en) 2012-02-02 2015-01-01 Corning Incorporated Automatic continuous perfusion cell culture microplate consumables
EP2834649A4 (en) 2012-04-01 2015-12-09 Emd Millipore Corp CELL CULTURE AND GRADIENT MIGRATION TEST METHOD AND DEVICES
US9946845B2 (en) 2012-10-02 2018-04-17 Rxsafe Llc System and method for filling and dispensing orders
SG10201901708SA (en) 2012-10-23 2019-03-28 Genzyme Corp Perfusion culturing methods and uses thereof
CA2901950A1 (en) 2013-02-22 2014-08-28 Genzyme Corporation Microcarrier perfusion culturing methods and uses thereof
WO2014130864A2 (en) 2013-02-22 2014-08-28 Genzyme Corporation Microcarrier perfusion culturing methods and uses thereof
MX2015012114A (es) 2013-03-08 2016-01-12 Genzyme Corp Fabricacion continua integrada de principios activos proteinicos terapeuticos.
CN112410279A (zh) 2013-03-15 2021-02-26 建新公司 高密度细胞存储方法
US9481901B2 (en) * 2013-05-30 2016-11-01 Amgen Inc. Methods for increasing mannose content of recombinant proteins
MX384020B (es) 2013-09-16 2025-03-14 Genzyme Corp Metodos y sistemas para el procesamiento de un cultivo celular.
JP2017500017A (ja) 2013-12-20 2017-01-05 バイオジェン・エムエイ・インコーポレイテッドBiogen MA Inc. 生物薬剤フェドバッチ生産能力及び生産物品質を改善するための灌流シード培養の使用
TWI709569B (zh) 2014-01-17 2020-11-11 美商健臻公司 無菌層析樹脂及其用於製造方法的用途
TWI671312B (zh) 2014-01-17 2019-09-11 美商健臻公司 無菌層析法及製法
FI3926051T3 (fi) 2014-06-04 2024-06-18 Amgen Inc Menetelmiä nisäkässoluviljelmien keräämiseksi
TWI743024B (zh) 2014-06-06 2021-10-21 美商健臻公司 灌注培養方法及其用途
TWI776235B (zh) 2014-06-09 2022-09-01 美商健臻公司 種子罐培養法(seed train processes)及其用途
CN104480897B (zh) 2014-11-28 2017-01-04 上海市政工程设计研究总院(集团)有限公司 陆域占地少应用范围广的防汛墙
CN104450597B (zh) 2014-12-10 2020-07-28 青岛农业大学 一种石油降解菌固体菌剂的制备方法及由其制备的固体菌剂修复石油污染的土壤的方法
CA2971861C (en) 2014-12-22 2024-02-13 Genzyme Corporation Methods of culturing a mammalian cell

Also Published As

Publication number Publication date
SG11201610167WA (en) 2017-01-27
US12006510B2 (en) 2024-06-11
WO2015188106A3 (en) 2016-01-28
CA2951255A1 (en) 2015-12-10
AU2015269184A1 (en) 2016-12-22
US20240287451A1 (en) 2024-08-29
AU2021205004A1 (en) 2021-09-23
KR102611855B1 (ko) 2023-12-07
KR102479281B1 (ko) 2022-12-20
US20160017280A1 (en) 2016-01-21
JP2017520242A (ja) 2017-07-27
TW202204596A (zh) 2022-02-01
IL249384A0 (en) 2017-02-28
TW201614062A (en) 2016-04-16
KR20230003634A (ko) 2023-01-06
RU2020131199A3 (es) 2022-03-22
RU2019138327A (ru) 2019-12-17
CN106795495A (zh) 2017-05-31
US20210324328A1 (en) 2021-10-21
RU2019138327A3 (es) 2020-04-02
RU2016152238A3 (es) 2019-05-30
US11060058B2 (en) 2021-07-13
TWI743024B (zh) 2021-10-21
BR112016028512A2 (pt) 2017-08-22
MX2016016142A (es) 2017-03-28
SG10202008131WA (en) 2020-10-29
JP2023120382A (ja) 2023-08-29
RU2708919C2 (ru) 2019-12-12
AU2015269184B2 (en) 2021-04-15
RU2733539C2 (ru) 2020-10-05
JP2021168681A (ja) 2021-10-28
JP7350810B2 (ja) 2023-09-26
BR112016028512A8 (pt) 2021-03-16
RU2020131199A (ru) 2022-03-22
RU2016152238A (ru) 2018-07-10
KR20170010892A (ko) 2017-02-01
JP6915992B2 (ja) 2021-08-11
EP3152293A2 (en) 2017-04-12
WO2015188106A2 (en) 2015-12-10

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