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SG11201610167WA - Perfusion culturing methods and uses thereof - Google Patents

Perfusion culturing methods and uses thereof

Info

Publication number
SG11201610167WA
SG11201610167WA SG11201610167WA SG11201610167WA SG11201610167WA SG 11201610167W A SG11201610167W A SG 11201610167WA SG 11201610167W A SG11201610167W A SG 11201610167WA SG 11201610167W A SG11201610167W A SG 11201610167WA SG 11201610167W A SG11201610167W A SG 11201610167WA
Authority
SG
Singapore
Prior art keywords
culturing methods
perfusion culturing
perfusion
methods
culturing
Prior art date
Application number
SG11201610167WA
Inventor
Agata Villiger-Oberbek
Jianguo Yang
Yang Yang
Gabrielle Lornzo
Original Assignee
Genzyme Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genzyme Corp filed Critical Genzyme Corp
Publication of SG11201610167WA publication Critical patent/SG11201610167WA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/26—Inoculator or sampler
    • C12M1/32—Inoculator or sampler multiple field or continuous type
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/045—Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/10—Rotating vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2527/00—Culture process characterised by the use of mechanical forces, e.g. strain, vibration

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
SG11201610167WA 2014-06-06 2015-06-05 Perfusion culturing methods and uses thereof SG11201610167WA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462009058P 2014-06-06 2014-06-06
PCT/US2015/034494 WO2015188106A2 (en) 2014-06-06 2015-06-05 Perfusion culturing methods and uses thereof

Publications (1)

Publication Number Publication Date
SG11201610167WA true SG11201610167WA (en) 2017-01-27

Family

ID=53484156

Family Applications (2)

Application Number Title Priority Date Filing Date
SG10202008131WA SG10202008131WA (en) 2014-06-06 2015-06-05 Perfusion culturing methods and uses thereof
SG11201610167WA SG11201610167WA (en) 2014-06-06 2015-06-05 Perfusion culturing methods and uses thereof

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG10202008131WA SG10202008131WA (en) 2014-06-06 2015-06-05 Perfusion culturing methods and uses thereof

Country Status (14)

Country Link
US (3) US11060058B2 (en)
EP (1) EP3152293B1 (en)
JP (3) JP6915992B2 (en)
KR (2) KR102479281B1 (en)
CN (1) CN106795495A (en)
AU (2) AU2015269184B2 (en)
BR (1) BR112016028512A8 (en)
CA (1) CA2951255A1 (en)
IL (1) IL249384A0 (en)
MX (2) MX2016016142A (en)
RU (3) RU2733539C2 (en)
SG (2) SG10202008131WA (en)
TW (2) TW202204596A (en)
WO (1) WO2015188106A2 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2717927T3 (en) 2013-02-22 2019-06-26 Genzyme Corp Cultivation procedures by perfusion of microcarriers and uses thereof
WO2014130872A2 (en) 2013-02-22 2014-08-28 Genzyme Corporation Microcarrier perfusion culturing methods and uses thereof
TW202204596A (en) * 2014-06-06 2022-02-01 美商健臻公司 Perfusion culturing methods and uses thereof
TWI707949B (en) 2014-06-09 2020-10-21 美商健臻公司 Seed train processes and uses thereof
KR102568951B1 (en) 2014-12-22 2023-08-22 젠자임 코포레이션 Methods for culturing mammalian cells
US11292999B2 (en) 2016-08-30 2022-04-05 Finesse Solutions, Inc. Bioreactor with multiple coupled vessels
EP3875595A4 (en) * 2018-11-02 2022-07-13 Kyowa Kirin Co., Ltd. PROCESS FOR PREPARING A LIQUID MEDIUM
WO2022173964A1 (en) * 2021-02-10 2022-08-18 Koniku Inc. Assay system, methods, and multi-well plate for gas stimulation of biological cells, proteins or materials
EP4323500A1 (en) * 2021-04-14 2024-02-21 Genzyme Corporation Methods of perfusion culturing a mammalian cell
JP2023058899A (en) * 2021-10-14 2023-04-26 株式会社三共 game machine
JP2023058903A (en) * 2021-10-14 2023-04-26 株式会社三共 game machine
JP2023058901A (en) * 2021-10-14 2023-04-26 株式会社三共 game machine
JP2023058902A (en) * 2021-10-14 2023-04-26 株式会社三共 game machine
JP2023058900A (en) * 2021-10-14 2023-04-26 株式会社三共 game machine
WO2024112917A1 (en) * 2022-11-22 2024-05-30 The University Of North Carolina At Chapel Hill Laminar flow incorporated scaffold-based 3d cell cultures
DE102022214275A1 (en) * 2022-12-22 2024-06-27 Robert Bosch Gesellschaft mit beschränkter Haftung Device and method for culturing cells
US20240263122A1 (en) * 2023-02-08 2024-08-08 National Central University Bioreactor apparatus, ion-exchange substrate and method for regulation of ion concentration and cell culture thereof

Family Cites Families (73)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4522811A (en) 1982-07-08 1985-06-11 Syntex (U.S.A.) Inc. Serial injection of muramyldipeptides and liposomes enhances the anti-infective activity of muramyldipeptides
RU2058992C1 (en) 1993-07-14 1996-04-27 Антипин Георгий Васильевич Method for production of feed protein and device for its embodiment
DE19612966B4 (en) 1996-04-01 2009-12-10 Novartis Vaccines And Diagnostics Gmbh & Co. Kg MDCK cells and methods of propagating influenza viruses
RU2215748C2 (en) 1999-04-09 2003-11-10 Сентро Де Инмунология Молекулар Method for preparing recombinant human erythropoietin and prepared erythropoietin, pharmaceutical composition
WO2000063403A2 (en) 1999-04-15 2000-10-26 Crucell Holland B.V. Recombinant protein production in a human cell using sequences encoding adenovirus e1 protein
AT409379B (en) * 1999-06-02 2002-07-25 Baxter Ag MEDIUM FOR PROTEIN- AND SERUM-FREE CELL CULTURE
UA74557C2 (en) 1999-09-03 2006-01-16 Applied Research Systems A method for producing a heterologous secreted protein from chinese hamster ovaries cells grown on microcarriers
US6585971B1 (en) 1999-11-12 2003-07-01 Harbor-Ucla Research And Education Institute Recombinant α-L-iduronidase, methods for producing and purifying the same and methods for treating disease caused by deficiencies thereof
JP4176472B2 (en) 2000-12-20 2008-11-05 バイエル・フアーマシユーチカルズ・コーポレーシヨン Apparatus and method for seed-train expansion of mammalian cells
US6544788B2 (en) 2001-02-15 2003-04-08 Vijay Singh Disposable perfusion bioreactor for cell culture
JP2005509406A (en) 2001-05-09 2005-04-14 バイエル・フアーマシユーチカルズ・コーポレーシヨン Cryopreservation bag assembly for mammalian cell lines
JP4279669B2 (en) 2001-08-31 2009-06-17 バイエル・ヘルスケア・アクチェンゲゼルシャフト Unit and method for performing high cell density fermentation
PL368119A1 (en) 2001-10-02 2005-03-21 Novo Nordisk Health Care Ag Method for production of recombinant proteins in eukaryote cells
EP1434953A1 (en) 2001-10-11 2004-07-07 Siemens Aktiengesellschaft Damping device and method for damping movements in a damping device
AU2002348151A1 (en) 2001-11-05 2003-05-19 Genvec, Inc. Viral vector production methods and compositions
US20030091976A1 (en) 2001-11-14 2003-05-15 Ciphergen Biosystems, Inc. Methods for monitoring polypeptide production and purification using surface enhanced laser desorption/ionization mass spectrometry
US6953692B2 (en) 2001-12-18 2005-10-11 Bayer Pharmaceuticals Corporation Device and method for seed-train expansion of mammalian cells
US20050019914A1 (en) 2003-07-24 2005-01-27 Aventis Pharma Deutschland Gmbh Perfusion process for producing erythropoietin
AR046510A1 (en) 2003-07-25 2005-12-14 Regeneron Pharma COMPOSITION OF A VEGF ANTAGONIST AND AN ANTI-PROLIFERATIVE AGENT
EP1508576A1 (en) 2003-08-20 2005-02-23 Crucell Holland B.V. Efficient production of IgA in recombinant mammalian cells
US20050186669A1 (en) 2004-02-20 2005-08-25 Cesco Bioengineering Co., Ltd. Apparatus and method for preparing and culturing cells
EP1773978B1 (en) 2004-05-19 2014-04-02 Massachusetts Institute Of Technology Perfused three-dimensional cell/tissue disease models
WO2006033935A2 (en) * 2004-09-16 2006-03-30 Becton, Dickinson And Company Perfusion bioreactor for culturing cells
US7767446B2 (en) * 2004-09-16 2010-08-03 Becton, Dickinson And Company Perfusion bioreactors for culturing cells
CN101076347B (en) 2004-09-30 2012-09-26 拜耳医药保健有限公司 Devices and methods for integrated continuous manufacturing of biological molecules
DK1891205T3 (en) 2005-06-15 2018-03-19 Zhejiang Jinyishengshi Bioengineering Co Ltd SUSPENSION CULTURE CONTAINERS
WO2006138143A1 (en) 2005-06-15 2006-12-28 Amprotein Corporation Suspension culture vessels
WO2008024769A2 (en) 2006-08-21 2008-02-28 Raven Biotechnologies, Inc. Intensified perfusion production method
US20080207487A1 (en) 2006-11-02 2008-08-28 Neose Technologies, Inc. Manufacturing process for the production of polypeptides expressed in insect cell-lines
US8058027B2 (en) 2007-01-08 2011-11-15 Millipore Corporation Cell culture methods for producing recombinant proteins in the presence of reduced levels of one or more contaminants
US8501462B2 (en) * 2007-02-27 2013-08-06 The Board Of Trustees Of The University Of Illinois Insert device for multiwell plate
US8221999B2 (en) 2007-03-02 2012-07-17 Boehringer Ingelheim Pharma Gmbh & Co. Kg Protein production
EP2155347B1 (en) 2007-04-17 2013-07-17 Xendo Holding B.V. Method and device for continuous membrane adsorption
TW200902708A (en) 2007-04-23 2009-01-16 Wyeth Corp Methods of protein production using anti-senescence compounds
US9109193B2 (en) 2007-07-30 2015-08-18 Ge Healthcare Bio-Sciences Corp. Continuous perfusion bioreactor system
EP2188371B1 (en) 2007-08-09 2017-12-20 Wyeth LLC Use of perfusion to enhance production of fed-batch cell culture in bioreactors
WO2009034186A2 (en) 2007-09-13 2009-03-19 Helmholtz-Zentrum für Infektionsforschung GmbH Process for cell cultivation
US20090233334A1 (en) 2008-03-11 2009-09-17 Excellgene Sa Cell cultivation and production of recombinant proteins by means of an orbital shake bioreactor system with disposable bags at the 1,500 liter scale
DE102008019691A1 (en) 2008-04-15 2009-10-29 Technische Universität Ilmenau Semi-active microfluidic system for 3D cell culture as well as procedures for its perfusion
CN101821378B (en) 2008-06-16 2015-07-08 浙江金仪盛世生物工程有限公司 Bioreactors
WO2010048417A2 (en) * 2008-10-22 2010-04-29 Biovest International, Inc. Perfusion bioreactors, cell culture systems, and methods for production of cells and cell-derived products
WO2010110754A1 (en) * 2009-03-26 2010-09-30 Agency For Science, Technology And Research Apparatus for cell or tissue culture
US8728479B2 (en) 2009-03-31 2014-05-20 The Trustees Of The University Of Pennsylvania Antigen-binding proteins comprising recombinant protein scaffolds
CN101875917B (en) 2009-04-28 2012-07-04 北京清大天一科技有限公司 Method for producing swine fever vaccines by using animal cells cultured by micro-carriers of bio-reactor
KR101838302B1 (en) 2009-07-06 2018-03-13 제넨테크, 인크. Method of culturing eukaryotic cells
EP2542662B1 (en) 2010-03-02 2017-12-06 Universite Technologie De Compiegne-UTC Multi-reactor unit for dynamic cell culture
CN201933090U (en) 2010-11-22 2011-08-17 朱学仁 Interception device for perfusion culture of cell microcarrier
EP3578522A1 (en) 2010-12-06 2019-12-11 Pall Corporation Continuous processing methods for biological products
PL2665806T3 (en) 2011-01-17 2021-12-27 F.Hoffmann-La Roche Ag Separation apparatus
CN103476919B (en) 2011-03-22 2016-03-16 株式会社东海希多 Observation by microscope culture apparatus and using method thereof
DK2707488T3 (en) 2011-05-12 2017-12-11 Probiogen Ag IMPROVEMENT OF PROTEIN PREPARATION YIELD MEDIATED BY CDC42 GTPASE ON FAST CYCLES
KR102108663B1 (en) 2011-07-01 2020-05-07 암젠 인크 Mammalian cell culture
WO2013011616A1 (en) * 2011-07-15 2013-01-24 パナソニック株式会社 Mobile terminal
DK2620561T3 (en) 2012-01-30 2015-02-16 Dannrup Neils Device and method for filtering surface
US20150004686A1 (en) * 2012-02-02 2015-01-01 Corning Incorporated Automatic continuous perfusion cell culture microplate consumables
SG10201607963SA (en) * 2012-04-01 2016-11-29 Emd Millipore Corp Cell culture and gradient migration assay method and devices
US9946845B2 (en) 2012-10-02 2018-04-17 Rxsafe Llc System and method for filling and dispensing orders
WO2014066519A1 (en) * 2012-10-23 2014-05-01 Genzyme Corporation Perfusion culturing methods and uses thereof
WO2014130872A2 (en) 2013-02-22 2014-08-28 Genzyme Corporation Microcarrier perfusion culturing methods and uses thereof
ES2717927T3 (en) 2013-02-22 2019-06-26 Genzyme Corp Cultivation procedures by perfusion of microcarriers and uses thereof
IL274646B (en) 2013-03-08 2022-09-01 Genzyme Corp Integrated continuous production of medicinal substances from medical protein
SG10201707554WA (en) 2013-03-15 2017-11-29 Genzyme Corp High-density cell banking methods
US9481901B2 (en) * 2013-05-30 2016-11-01 Amgen Inc. Methods for increasing mannose content of recombinant proteins
JP6925127B2 (en) 2013-09-16 2021-08-25 ジェンザイム・コーポレーション Methods and systems for processing cell cultures
US20160289633A1 (en) 2013-12-20 2016-10-06 Biogen Ma Inc. Use of Perfusion Seed Cultures to Improve Biopharmaceutical Fed-Batch Production Capacity and Product Quality
TWI709570B (en) 2014-01-17 2020-11-11 美商健臻公司 Sterile chromatography and manufacturing processes
TWI709569B (en) 2014-01-17 2020-11-11 美商健臻公司 Sterile chromatography resin and use thereof in manufacturing processes
CA3195249A1 (en) 2014-06-04 2015-12-10 Amgen Inc. Methods for harvesting mammalian cell cultures
TW202204596A (en) * 2014-06-06 2022-02-01 美商健臻公司 Perfusion culturing methods and uses thereof
TWI707949B (en) 2014-06-09 2020-10-21 美商健臻公司 Seed train processes and uses thereof
CN104480897B (en) 2014-11-28 2017-01-04 上海市政工程设计研究总院（集团）有限公司 Flood prevention wall with small land occupation and wide application range
CN104450597B (en) 2014-12-10 2020-07-28 青岛农业大学 Preparation method of petroleum degrading bacteria solid microbial inoculum and method for repairing petroleum-polluted soil by using solid microbial inoculum prepared by preparation method
KR102568951B1 (en) 2014-12-22 2023-08-22 젠자임 코포레이션 Methods for culturing mammalian cells

Also Published As

Publication number Publication date
TWI743024B (en) 2021-10-21
WO2015188106A2 (en) 2015-12-10
WO2015188106A3 (en) 2016-01-28
MX2016016142A (en) 2017-03-28
RU2019138327A (en) 2019-12-17
JP2023120382A (en) 2023-08-29
AU2015269184A1 (en) 2016-12-22
CA2951255A1 (en) 2015-12-10
JP2017520242A (en) 2017-07-27
KR20230003634A (en) 2023-01-06
US11060058B2 (en) 2021-07-13
US20210324328A1 (en) 2021-10-21
MX2020009670A (en) 2020-10-08
RU2020131199A (en) 2022-03-22
JP6915992B2 (en) 2021-08-11
TW201614062A (en) 2016-04-16
RU2708919C2 (en) 2019-12-12
RU2020131199A3 (en) 2022-03-22
JP7350810B2 (en) 2023-09-26
EP3152293A2 (en) 2017-04-12
KR20170010892A (en) 2017-02-01
BR112016028512A8 (en) 2021-03-16
RU2733539C2 (en) 2020-10-05
US12006510B2 (en) 2024-06-11
KR102611855B1 (en) 2023-12-07
RU2016152238A3 (en) 2019-05-30
BR112016028512A2 (en) 2017-08-22
JP2021168681A (en) 2021-10-28
AU2021205004A1 (en) 2021-09-23
US20240287451A1 (en) 2024-08-29
RU2016152238A (en) 2018-07-10
IL249384A0 (en) 2017-02-28
EP3152293B1 (en) 2025-07-30
CN106795495A (en) 2017-05-31
RU2019138327A3 (en) 2020-04-02
US20160017280A1 (en) 2016-01-21
AU2015269184B2 (en) 2021-04-15
KR102479281B1 (en) 2022-12-20
SG10202008131WA (en) 2020-10-29
TW202204596A (en) 2022-02-01

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