BR112012020975A2 - "método para a construção de bactéria pertencente ao gênero bifidobacterium" - Google Patents
"método para a construção de bactéria pertencente ao gênero bifidobacterium"Info
- Publication number
- BR112012020975A2 BR112012020975A2 BR112012020975A BR112012020975A BR112012020975A2 BR 112012020975 A2 BR112012020975 A2 BR 112012020975A2 BR 112012020975 A BR112012020975 A BR 112012020975A BR 112012020975 A BR112012020975 A BR 112012020975A BR 112012020975 A2 BR112012020975 A2 BR 112012020975A2
- Authority
- BR
- Brazil
- Prior art keywords
- bacteria belonging
- genus bifidobacterium
- construction
- bacteria
- subculturing
- Prior art date
Links
- 241000894006 Bacteria Species 0.000 title abstract 6
- 241000186000 Bifidobacterium Species 0.000 title abstract 6
- 238000000034 method Methods 0.000 title abstract 5
- 238000010276 construction Methods 0.000 title abstract 2
- 230000007613 environmental effect Effects 0.000 abstract 2
- 230000035899 viability Effects 0.000 abstract 2
- 230000014670 detection of bacterium Effects 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/123—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
- A23C9/1234—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt characterised by using a Lactobacillus sp. other than Lactobacillus Bulgaricus, including Bificlobacterium sp.
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/123—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
- A23C9/1236—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt using Leuconostoc, Pediococcus or Streptococcus sp. other than Streptococcus Thermophilus; Artificial sour buttermilk in general
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/36—Adaptation or attenuation of cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/21—Streptococcus, lactococcus
- A23V2400/231—Lactis
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/517—Bifidum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/519—Breve
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Nutrition Science (AREA)
- Cell Biology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Dairy Products (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
método para a construção de bactéria pertencente ao gênero bifidobacterium. um método para a produção de bactérias pertencentes ao gênero bifidobacterium possuindo excelente viabilidade, mesmo sob diversas condições diferentes, com diferentes fatores ambientais, as bactérias pertencendo ao gênero bifidobacterium obtidas pelo método e um método para a detecção das bactérias são fornecidos. mediante repicagem a armazenamento das bactérias pertencentes ao gênero bifidobacterium alternadamente em sistemas sob condições com diferentes fatores ambientais, as bactérias pertencentes ao gênero bifidobacterium exibem excelente viabilidade sob a totalidade das condições utilizadas para a alternada repicagem e armazenando podem ser produzidas.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2010039212 | 2010-02-24 | ||
JP2010-039212 | 2010-02-24 | ||
JP2010136792 | 2010-06-16 | ||
JP2010-136792 | 2010-06-16 | ||
PCT/JP2011/053737 WO2011105335A1 (ja) | 2010-02-24 | 2011-02-21 | 新規ビフィドバクテリウム属細菌の作出方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
BR112012020975A2 true BR112012020975A2 (pt) | 2015-11-03 |
BR112012020975B1 BR112012020975B1 (pt) | 2020-12-01 |
Family
ID=44506743
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112012020975-2A BR112012020975B1 (pt) | 2010-02-24 | 2011-02-21 | bactéria pertencente ao gênero bifidobacterium, alimento ou bebida, usos de um fragmento de dna e de um conjunto de iniciadores e métodos para a detecção e quantificação da dita bactéria |
Country Status (17)
Country | Link |
---|---|
US (1) | US8889120B2 (pt) |
EP (1) | EP2540816B1 (pt) |
JP (1) | JP5643285B2 (pt) |
KR (1) | KR101838762B1 (pt) |
CN (1) | CN102770528B (pt) |
AR (1) | AR081151A1 (pt) |
AU (1) | AU2011218925B2 (pt) |
BR (1) | BR112012020975B1 (pt) |
CA (1) | CA2790414C (pt) |
ES (1) | ES2526414T3 (pt) |
MX (1) | MX2012009799A (pt) |
MY (1) | MY158274A (pt) |
NZ (1) | NZ601532A (pt) |
PH (1) | PH12012501595A1 (pt) |
SG (1) | SG183481A1 (pt) |
TW (1) | TWI495722B (pt) |
WO (1) | WO2011105335A1 (pt) |
Families Citing this family (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
MX2012007873A (es) * | 2010-01-06 | 2012-07-25 | Yakult Honsha Kk | Promotor de reparacion del daño de acido desoxirribonucleico para aplicacion oral e inhibidor de la actividad de elastasa para apalicacion oral. |
JP6032737B2 (ja) * | 2012-09-27 | 2016-11-30 | 株式会社明治 | ビフィズス菌の検出方法 |
US20150284675A1 (en) * | 2012-10-25 | 2015-10-08 | Compagnie Gervais Danone | Streptococcus thermophilus strains for treating helicobacter pylori infection |
JP6412865B2 (ja) * | 2013-03-13 | 2018-10-24 | 株式会社ヤクルト本社 | ビフィドバクテリウム・ブレーベ株特異的遺伝子 |
US9957496B2 (en) | 2013-12-04 | 2018-05-01 | Kabushiki Kaisha Yakult Honsha | Method for regulating acid resistance of microbes |
WO2016080448A1 (ja) | 2014-11-19 | 2016-05-26 | 株式会社ヤクルト本社 | セリアック病予防治療剤 |
WO2017010001A1 (ja) * | 2015-07-16 | 2017-01-19 | 森永乳業株式会社 | 微生物検出法及び微生物検出キット |
WO2019215873A1 (ja) * | 2018-05-10 | 2019-11-14 | 森永乳業株式会社 | 生菌数の測定方法 |
JP7489045B2 (ja) * | 2018-09-25 | 2024-05-23 | 国立研究開発法人国立循環器病研究センター | 抗腫瘍効果増強剤 |
SG11202108657YA (en) * | 2019-02-19 | 2021-09-29 | Yakult Honsha Kk | Method for producing culture of lactic acid bacterium and/or bacterium belonging to genus bifidobacterium |
JP7309412B2 (ja) * | 2019-03-29 | 2023-07-18 | 株式会社ヤクルト本社 | 乳酸菌発酵食品の製造方法 |
JP7523898B2 (ja) * | 2019-09-30 | 2024-07-29 | キリンホールディングス株式会社 | 発酵工程中の機能性ペプチド含有量の減少が抑制された、はっ酵乳の製造方法 |
BR112022007564A2 (pt) * | 2019-11-11 | 2022-07-05 | Yakult Honsha Kk | Método para produção de leite fermentado contendo oligossacarídeos |
KR102265807B1 (ko) * | 2020-03-03 | 2021-06-16 | 나종주 | 생균제를 이용한 사료첨가제의 제조방법 |
CN114369547B (zh) * | 2021-12-30 | 2023-10-20 | 黑龙江飞鹤乳业有限公司 | 一种复合乳酸菌、发酵乳及在缓解便秘中的应用 |
TW202444888A (zh) * | 2023-03-31 | 2024-11-16 | 日商養樂多本社股份有限公司 | 新穎雙叉桿菌屬細菌 |
Family Cites Families (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7015A (en) * | 1850-01-15 | Dampeb fob | ||
JPS5283975A (en) | 1976-01-01 | 1977-07-13 | Yakult Honsha Kk | Method of producing fermented milk product containg living cell of bifidus strain |
JPS5953829B2 (ja) * | 1982-06-22 | 1984-12-27 | 森永乳業株式会社 | ビフィドバクテリウム・ロンガムに属する新規微生物、その菌体の製造法および該微生物を含む組成物 |
JPS6119220A (ja) | 1984-07-05 | 1986-01-28 | Sony Corp | 選局装置 |
JPS62268A (ja) * | 1985-06-26 | 1987-01-06 | Meiji Milk Prod Co Ltd | 変異株ラクトバチルス・ブルガリクス及びその選択方法 |
US4734361A (en) | 1985-06-26 | 1988-03-29 | Meiji Milk Products Co., Ltd. | Low temperature-sensitive variant of lactobacillus bulgaricus and a selection method therefor |
JPS62628A (ja) | 1985-06-26 | 1987-01-06 | Fuji Heavy Ind Ltd | 高温再始動表示装置 |
JP2922013B2 (ja) * | 1991-04-17 | 1999-07-19 | 雪印ローリー株式会社 | 耐酸性及び酸素耐性を有する新規ビフィズス菌 |
TW357191B (en) | 1996-06-04 | 1999-05-01 | Food Industry Development Res Institute | Bifidobacteria strains with acid, bile salt and oxygen tolerance and their culture method the invention relates to bifidobacteria strains with acid, bile salt and oxygen tolerance and their culture method |
JP3068484B2 (ja) | 1997-02-17 | 2000-07-24 | 株式会社ヤクルト本社 | ビフィズス菌発酵乳及びその製造法 |
JP4046389B2 (ja) | 1997-09-04 | 2008-02-13 | 株式会社ヤクルト本社 | ビフィドバクテリウム・ブレーベ及びこれを用いた発酵豆乳 |
JP3749359B2 (ja) * | 1997-09-16 | 2006-02-22 | 雪印乳業株式会社 | 新規ビフィズス菌及びその利用 |
US20070150978A1 (en) | 1999-05-17 | 2007-06-28 | Byrum Joseph R | Nucleic acid molecules and other molecules associated with plants |
JP2001112437A (ja) * | 1999-10-18 | 2001-04-24 | Yakult Honsha Co Ltd | ビフィドバクテリウム属細菌含有飲食品の製造方法 |
WO2002061118A1 (fr) | 2001-02-01 | 2002-08-08 | Kabushiki Kaisha Yakult Honsha | Methode d'evaluation de l'acces a l'intestin d'une bacterie bifidus contenue dans un aliment ou une boisson a base de lait fermente |
JP3905082B2 (ja) | 2001-11-05 | 2007-04-18 | 株式会社ヤクルト本社 | ビフィドバクテリウム属細菌及びこれを用いた発酵食品 |
JP2003250528A (ja) * | 2002-03-06 | 2003-09-09 | Yakult Honsha Co Ltd | ビフィドバクテリウム属細菌の生残性改善剤、増殖促進剤、又は、同細菌含有醗酵物の製造方法 |
JP2004135535A (ja) * | 2002-10-16 | 2004-05-13 | Aaku Giken:Kk | 乳酸菌生成物とその製造方法 |
US8058051B2 (en) | 2005-07-21 | 2011-11-15 | Kabushiki Kaisha Yakult Honsha | Bacterium belonging to the genus Bifidobacterium and utilization of the same |
-
2011
- 2011-02-21 KR KR1020127021944A patent/KR101838762B1/ko active Active
- 2011-02-21 AU AU2011218925A patent/AU2011218925B2/en active Active
- 2011-02-21 SG SG2012062840A patent/SG183481A1/en unknown
- 2011-02-21 CN CN201180010882.4A patent/CN102770528B/zh active Active
- 2011-02-21 MX MX2012009799A patent/MX2012009799A/es active IP Right Grant
- 2011-02-21 CA CA2790414A patent/CA2790414C/en active Active
- 2011-02-21 JP JP2012501772A patent/JP5643285B2/ja active Active
- 2011-02-21 ES ES11747296.9T patent/ES2526414T3/es active Active
- 2011-02-21 US US13/577,511 patent/US8889120B2/en active Active
- 2011-02-21 EP EP11747296.9A patent/EP2540816B1/en active Active
- 2011-02-21 PH PH1/2012/501595A patent/PH12012501595A1/en unknown
- 2011-02-21 NZ NZ601532A patent/NZ601532A/en unknown
- 2011-02-21 WO PCT/JP2011/053737 patent/WO2011105335A1/ja active Application Filing
- 2011-02-21 MY MYPI2012003773A patent/MY158274A/en unknown
- 2011-02-21 BR BR112012020975-2A patent/BR112012020975B1/pt active IP Right Grant
- 2011-02-24 TW TW100106288A patent/TWI495722B/zh active
- 2011-02-24 AR ARP110100577A patent/AR081151A1/es unknown
Also Published As
Publication number | Publication date |
---|---|
CA2790414C (en) | 2016-10-04 |
ES2526414T3 (es) | 2015-01-12 |
US20120329059A1 (en) | 2012-12-27 |
NZ601532A (en) | 2014-03-28 |
AR081151A1 (es) | 2012-07-04 |
KR20130004572A (ko) | 2013-01-11 |
AU2011218925A1 (en) | 2012-08-23 |
TW201142019A (en) | 2011-12-01 |
WO2011105335A1 (ja) | 2011-09-01 |
HK1174952A1 (en) | 2013-06-21 |
AU2011218925B2 (en) | 2014-12-04 |
SG183481A1 (en) | 2012-10-30 |
MY158274A (en) | 2016-09-30 |
TWI495722B (zh) | 2015-08-11 |
EP2540816A4 (en) | 2013-03-20 |
EP2540816B1 (en) | 2014-12-03 |
CA2790414A1 (en) | 2011-09-01 |
MX2012009799A (es) | 2012-09-12 |
BR112012020975B1 (pt) | 2020-12-01 |
PH12012501595A1 (en) | 2016-05-25 |
EP2540816A1 (en) | 2013-01-02 |
CN102770528A (zh) | 2012-11-07 |
US8889120B2 (en) | 2014-11-18 |
KR101838762B1 (ko) | 2018-03-14 |
CN102770528B (zh) | 2014-07-16 |
JP5643285B2 (ja) | 2014-12-17 |
JPWO2011105335A1 (ja) | 2013-06-20 |
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