ATE557083T1 - Thermostabiler oder thermoactiver dna-polymerase mit abgeschwächter 3'-5' exonukleaseaktivität - Google Patents
Thermostabiler oder thermoactiver dna-polymerase mit abgeschwächter 3'-5' exonukleaseaktivitätInfo
- Publication number
- ATE557083T1 ATE557083T1 AT10172676T AT10172676T ATE557083T1 AT E557083 T1 ATE557083 T1 AT E557083T1 AT 10172676 T AT10172676 T AT 10172676T AT 10172676 T AT10172676 T AT 10172676T AT E557083 T1 ATE557083 T1 AT E557083T1
- Authority
- AT
- Austria
- Prior art keywords
- thermostable
- attenuated
- dna polymerase
- exonuclease activity
- thermoactive dna
- Prior art date
Links
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 title abstract 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 title abstract 2
- 108060002716 Exonuclease Proteins 0.000 title abstract 2
- 230000002238 attenuated effect Effects 0.000 title abstract 2
- 230000000694 effects Effects 0.000 title abstract 2
- 102000013165 exonuclease Human genes 0.000 title abstract 2
- 238000000034 method Methods 0.000 abstract 3
- 238000003752 polymerase chain reaction Methods 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 108020004511 Recombinant DNA Proteins 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 230000001915 proofreading effect Effects 0.000 abstract 1
- 238000003786 synthesis reaction Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US36981502P | 2002-04-02 | 2002-04-02 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE557083T1 true ATE557083T1 (de) | 2012-05-15 |
Family
ID=28042066
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT10172676T ATE557083T1 (de) | 2002-04-02 | 2003-03-31 | Thermostabiler oder thermoactiver dna-polymerase mit abgeschwächter 3'-5' exonukleaseaktivität |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US7148049B2 (de) |
| EP (2) | EP2272952B8 (de) |
| JP (2) | JP4034684B2 (de) |
| CN (1) | CN1482240B (de) |
| AT (1) | ATE557083T1 (de) |
| ES (1) | ES2389711T3 (de) |
Families Citing this family (45)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7947817B2 (en) * | 2003-06-30 | 2011-05-24 | Roche Molecular Systems, Inc. | Synthesis and compositions of 2'-terminator nucleotides |
| JP2007159401A (ja) * | 2004-05-07 | 2007-06-28 | Precision System Science Co Ltd | 改変タンパク質の作製方法 |
| EP1757698B1 (de) * | 2004-06-04 | 2012-05-23 | Takara Bio, Inc. | Polypeptide mit dna-polymerase-aktivität |
| US7745125B2 (en) | 2004-06-28 | 2010-06-29 | Roche Molecular Systems, Inc. | 2′-terminator related pyrophosphorolysis activated polymerization |
| US20070048748A1 (en) * | 2004-09-24 | 2007-03-01 | Li-Cor, Inc. | Mutant polymerases for sequencing and genotyping |
| US7900380B2 (en) * | 2005-10-13 | 2011-03-08 | Masterfit Enterprises Inc. | User moldable adjustable insert |
| US8962293B2 (en) | 2006-10-18 | 2015-02-24 | Roche Molecular Systems, Inc. | DNA polymerases and related methods |
| US8026091B2 (en) * | 2007-07-13 | 2011-09-27 | Roche Molecular Systems, Inc. | DNA polymerases and related methods |
| WO2009068268A1 (en) * | 2007-11-28 | 2009-06-04 | Roche Diagnostics Gmbh | Mutant dna polymerases with improved pyrophosphorolysis activated polymerization (pap) ability |
| US10150990B2 (en) | 2008-04-21 | 2018-12-11 | Roche Molecular Systems, Inc. | Ribonucleotide tag nucleic acid detection |
| WO2010082640A1 (ja) * | 2009-01-15 | 2010-07-22 | 北海道三井化学株式会社 | 耐熱性dnaポリメラーゼを含む酵素調製物およびその製造方法、並びに検出対象生物の検出方法 |
| US9238832B2 (en) | 2009-12-11 | 2016-01-19 | Roche Molecular Systems, Inc. | Allele-specific amplification of nucleic acids |
| GB201007384D0 (en) * | 2010-04-30 | 2010-06-16 | Medical Res Council | Enzymes |
| JP5876477B2 (ja) | 2010-06-18 | 2016-03-02 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 増大した３’末端ミスマッチ識別能を有するｄｎａポリメラーゼ |
| US8722380B2 (en) | 2010-06-18 | 2014-05-13 | Roche Molecular Systems, Inc. | DNA polymerases with increased 3′-mismatch discrimination |
| JP5926248B2 (ja) | 2010-06-18 | 2016-05-25 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 増大した３’末端ミスマッチ識別能を有するｄｎａポリメラーゼ |
| CN103025869B (zh) | 2010-06-18 | 2015-07-01 | 霍夫曼-拉罗奇有限公司 | 具有增强的3’-错配辨别力的dna聚合酶 |
| EP2582801B1 (de) | 2010-06-18 | 2015-04-22 | Roche Diagnostics GmbH | Dna polymerasen mit erhöhter unterscheidung von 3' fehlpaarungen |
| WO2011157436A1 (en) | 2010-06-18 | 2011-12-22 | Roche Diagnostics Gmbh | Dna polymerases with increased 3'-mismatch discrimination |
| WO2011157435A1 (en) | 2010-06-18 | 2011-12-22 | Roche Diagnostics Gmbh | Dna polymerases with increased 3'-mismatch discrimination |
| US8715991B2 (en) | 2010-06-18 | 2014-05-06 | Roche Molecular Systems, Inc. | DNA polymerases with increased 3'-mismatch discrimination |
| WO2012046140A1 (en) | 2010-10-05 | 2012-04-12 | Finnzymes Oy | Enzyme mixture |
| US8765435B2 (en) | 2011-02-15 | 2014-07-01 | Roche Molecular Systems, Inc. | DNA polymerases with increased 3′-mismatch discrimination |
| WO2012139748A1 (en) | 2011-04-11 | 2012-10-18 | Roche Diagnostics Gmbh | Dna polymerases with improved activity |
| WO2012162621A1 (en) * | 2011-05-26 | 2012-11-29 | Brandeis University | Methods for suppression pcr |
| US9090883B2 (en) | 2011-07-28 | 2015-07-28 | Roche Molecular Systems, Inc. | DNA polymerases with improved activity |
| JP6144697B2 (ja) | 2011-12-08 | 2017-06-07 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 改善された活性を有するｄｎａポリメラーゼ |
| EP2788479B1 (de) | 2011-12-08 | 2016-02-10 | Roche Diagnostics GmbH | Dna-polymerasen mit verbesserter aktivität |
| ES2716277T3 (es) | 2011-12-08 | 2019-06-11 | Hoffmann La Roche | ADN polimerasas con actividad mejorada |
| WO2014150851A1 (en) | 2013-03-15 | 2014-09-25 | Ibis Biosciences, Inc. | Nucleotide analogs for sequencing |
| RU2688435C2 (ru) | 2013-08-19 | 2019-05-21 | Эбботт Молекьюлар Инк. | Набор для получения реакционной смеси для синтеза 3′-o-пропаргил-модифицированной нуклеиновой кислоты |
| CN104152426A (zh) * | 2014-08-05 | 2014-11-19 | 孙启明 | 一种t5核酸外切酶在大肠杆菌中的制备方法 |
| CN104313132A (zh) * | 2014-09-28 | 2015-01-28 | 南京诺唯赞生物科技有限公司 | 一种t4 dna聚合酶活性测定方法 |
| GB2542391A (en) * | 2015-09-17 | 2017-03-22 | Annexin Pharmaceuticals Ab | Process of manufacture |
| EP3405578B1 (de) | 2016-01-19 | 2022-03-16 | Board of Regents, The University of Texas System | Temperaturstabile reverse transkriptase |
| EP3562962B1 (de) * | 2016-12-30 | 2022-01-05 | Omniome, Inc. | Verfahren und system unter verwendung von unterscheidbaren polymerasen zum nachweis von ternären komplexen und zur identifizierung von verwandten nukleotiden |
| WO2018148726A1 (en) * | 2017-02-13 | 2018-08-16 | Qiagen Waltham Inc. | Polymerase enzyme from phage t4 |
| US20200002689A1 (en) | 2017-02-13 | 2020-01-02 | Qiagen Sciences, Llc | Polymerase enzyme from 9°n |
| EP3692049A4 (de) * | 2017-10-04 | 2021-06-23 | Centrillion Technology Holdings Corporation | Verfahren und system zur enzymatischen synthese von oligonukleotiden |
| TWI680168B (zh) * | 2017-10-18 | 2019-12-21 | 環球晶圓股份有限公司 | 碳化矽晶片 |
| WO2019084538A1 (en) | 2017-10-27 | 2019-05-02 | Board Of Regents, The University Of Texas System | TUMOR SPECIFIC ANTIBODIES, T CELL RECEPTORS AND METHODS OF IDENTIFICATION THEREOF |
| JP7218558B2 (ja) * | 2017-12-19 | 2023-02-07 | 東ソー株式会社 | 核酸増幅法および核酸増幅試薬 |
| ES2969883T3 (es) | 2018-03-21 | 2024-05-23 | Hoffmann La Roche | ADN polimerasas para la incorporación eficiente y eficaz de dNTP metilados |
| JP7504872B2 (ja) | 2018-09-13 | 2024-06-24 | エフ． ホフマン－ラ ロシュ アーゲー | 改善されたストランド置換能力を有する変異体ｄｎａポリメラーゼ |
| WO2021064106A1 (en) * | 2019-10-01 | 2021-04-08 | Universitetet I Tromsø - Norges Arktiske Universitet | Dna polymerase and dna polymerase derived 3'-5'exonuclease |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK149867C (da) | 1979-08-08 | 1987-04-06 | New Zealand Dev Finance | Proteolytisk enzympraeparat og fremgangsmaade til fremstilling deraf |
| US4399216A (en) | 1980-02-25 | 1983-08-16 | The Trustees Of Columbia University | Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials |
| US4458066A (en) | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
| US4419446A (en) | 1980-12-31 | 1983-12-06 | The United States Of America As Represented By The Department Of Health And Human Services | Recombinant DNA process utilizing a papilloma virus DNA as a vector |
| US4601978A (en) | 1982-11-24 | 1986-07-22 | The Regents Of The University Of California | Mammalian metallothionein promoter system |
| US4711845A (en) | 1984-08-31 | 1987-12-08 | Cetus Corporation | Portable temperature-sensitive control cassette |
| US4666848A (en) | 1984-08-31 | 1987-05-19 | Cetus Corporation | Polypeptide expression using a portable temperature sensitive control cassette with a positive retroregulatory element |
| EP0196864A3 (de) | 1985-03-25 | 1988-03-23 | Cetus Corporation | Behandlung und Sekretion von rekombinanten Proteinen mittels alkalischer Phosphatase, dazu verwendbare DNA-Sequenzen und mittels dieser Sequenzen transformierte Zellen |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| US4889818A (en) | 1986-08-22 | 1989-12-26 | Cetus Corporation | Purified thermostable enzyme |
| US5405774A (en) | 1986-08-22 | 1995-04-11 | Hoffmann-La Roche Inc. | DNA encoding a mutated thermostable nucleic acid polymerase enzyme from thermus species sps17 |
| US5310652A (en) | 1986-08-22 | 1994-05-10 | Hoffman-La Roche Inc. | Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription |
| US5374553A (en) * | 1986-08-22 | 1994-12-20 | Hoffmann-La Roche Inc. | DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima |
| US5079352A (en) | 1986-08-22 | 1992-01-07 | Cetus Corporation | Purified thermostable enzyme |
| US5795762A (en) | 1986-08-22 | 1998-08-18 | Roche Molecular Systems, Inc. | 5' to 3' exonuclease mutations of thermostable DNA polymerases |
| US5352600A (en) | 1986-08-22 | 1994-10-04 | Hoffmann-La Roche Inc. | Purified thermostable enzyme |
| US5466591A (en) | 1986-08-22 | 1995-11-14 | Hoffmann-La Roche Inc. | 5' to 3' exonuclease mutations of thermostable DNA polymerases |
| US5618711A (en) | 1986-08-22 | 1997-04-08 | Hoffmann-La Roche Inc. | Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase |
| US5455170A (en) | 1986-08-22 | 1995-10-03 | Hoffmann-La Roche Inc. | Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05 |
| US5352778A (en) | 1990-04-26 | 1994-10-04 | New England Biolabs, Inc. | Recombinant thermostable DNA polymerase from archaebacteria |
| US5322785A (en) | 1990-04-26 | 1994-06-21 | New England Biolabs, Inc. | Purified thermostable DNA polymerase obtainable from thermococcus litoralis |
| US5210036A (en) | 1990-04-26 | 1993-05-11 | New England Biolabs, Inc. | Purified thermostable DNA polymerase obtainable from thermococcus litoralis |
| ATE197718T1 (de) | 1990-09-28 | 2000-12-15 | Hoffmann La Roche | Gereinigte thermostabile nukleinsäurepolymerase aus thermosipho africanus |
| US5489523A (en) | 1990-12-03 | 1996-02-06 | Stratagene | Exonuclease-deficient thermostable Pyrococcus furiosus DNA polymerase I |
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| US5556772A (en) | 1993-12-08 | 1996-09-17 | Stratagene | Polymerase compositions and uses thereof |
| US5512462A (en) | 1994-02-25 | 1996-04-30 | Hoffmann-La Roche Inc. | Methods and reagents for the polymerase chain reaction amplification of long DNA sequences |
| US6100078A (en) | 1994-04-01 | 2000-08-08 | Gen-Probe Incorporated | Purified DNA polymerase from bacillus stearothermophilus ATCC 12980 |
| US5912155A (en) | 1994-09-30 | 1999-06-15 | Life Technologies, Inc. | Cloned DNA polymerases from Thermotoga neapolitana |
| US6015668A (en) | 1994-09-30 | 2000-01-18 | Life Technologies, Inc. | Cloned DNA polymerases from thermotoga and mutants thereof |
| US5614365A (en) | 1994-10-17 | 1997-03-25 | President & Fellow Of Harvard College | DNA polymerase having modified nucleotide binding site for DNA sequencing |
| US5834253A (en) | 1994-11-17 | 1998-11-10 | Shanghai Institute Of Biochemistry, Chinese Academy Of Sciences | Bacillus stearothermophilus DNA polymerase with proof-reading 3'-5' exonuclease activity |
| CN1123328A (zh) | 1994-11-17 | 1996-05-29 | 中国科学院上海生物化学研究所 | 高温、高传真脱氧核糖核酸(DNA)聚合酶(HiFi Bst) |
| US5602011A (en) | 1995-01-18 | 1997-02-11 | Pharmacia Biotech Inc. | Purified Thermococcus barossii DNA polymerase |
| US6077664A (en) | 1995-06-07 | 2000-06-20 | Promega Corporation | Thermophilic DNA polymerases from Thermotoga neapolitana |
| US6001645A (en) | 1995-06-07 | 1999-12-14 | Promega Corporation | Thermophilic DNA polymerases from thermotoga neapolitana |
| AU7236296A (en) * | 1995-09-08 | 1997-03-27 | Life Technologies, Inc. | Cloned dna polymerases from thermotoga and mutants thereof |
| US5830714A (en) | 1996-04-17 | 1998-11-03 | Molecular Biology Resources, Inc. | Biologically active fragment of bacillus stearothermophilus DNA polymerase |
| CZ293215B6 (cs) | 1996-08-06 | 2004-03-17 | F. Hoffmann-La Roche Ag | Rekombinantní tepelně stálá DNA polymeráza, způsob její přípravy a prostředek, který ji obsahuje |
| SG86311A1 (en) | 1997-04-10 | 2002-02-19 | Univ Singapore | Bacillus stearothermophilus dna polymerase i (klenow) clones with reduced 3'-to-5' exonuclease activity |
| US6218150B1 (en) * | 1997-06-26 | 2001-04-17 | Takara Shuzo Co., Ltd. | DNA polymerase-related factors |
| US6228628B1 (en) * | 1997-07-09 | 2001-05-08 | Roche Molecular Systems | Mutant chimeric DNA polymerase |
| US5882904A (en) | 1997-08-04 | 1999-03-16 | Amersham Pharmacia Biotech Inc. | Thermococcus barossii DNA polymerase mutants |
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| US6183998B1 (en) | 1998-05-29 | 2001-02-06 | Qiagen Gmbh Max-Volmer-Strasse 4 | Method for reversible modification of thermostable enzymes |
| US6214557B1 (en) | 2000-06-06 | 2001-04-10 | Washington University | Cold sensitive mutant DNA polymerases |
-
2003
- 2003-03-26 US US10/401,403 patent/US7148049B2/en not_active Expired - Lifetime
- 2003-03-31 EP EP10172676A patent/EP2272952B8/de not_active Expired - Lifetime
- 2003-03-31 AT AT10172676T patent/ATE557083T1/de active
- 2003-03-31 ES ES10172676T patent/ES2389711T3/es not_active Expired - Lifetime
- 2003-03-31 EP EP20030006888 patent/EP1350841B1/de not_active Expired - Lifetime
- 2003-04-02 JP JP2003099762A patent/JP4034684B2/ja not_active Expired - Lifetime
- 2003-04-02 CN CN031312330A patent/CN1482240B/zh not_active Expired - Lifetime
-
2006
- 2006-08-24 JP JP2006228285A patent/JP4585996B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| CN1482240B (zh) | 2012-09-05 |
| JP4585996B2 (ja) | 2010-11-24 |
| EP1350841A3 (de) | 2003-12-10 |
| JP4034684B2 (ja) | 2008-01-16 |
| EP1350841A2 (de) | 2003-10-08 |
| EP2272952B1 (de) | 2012-05-09 |
| EP1350841B1 (de) | 2015-05-06 |
| JP2006311867A (ja) | 2006-11-16 |
| EP2272952B8 (de) | 2012-08-15 |
| US20040005599A1 (en) | 2004-01-08 |
| US7148049B2 (en) | 2006-12-12 |
| ES2389711T3 (es) | 2012-10-30 |
| EP2272952A1 (de) | 2011-01-12 |
| CN1482240A (zh) | 2004-03-17 |
| JP2003304870A (ja) | 2003-10-28 |
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