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ATE466080T1 - Formulierungen und verfahren zum denaturieren von proteinen - Google Patents

Formulierungen und verfahren zum denaturieren von proteinen

Info

Publication number
ATE466080T1
ATE466080T1 AT04814250T AT04814250T ATE466080T1 AT E466080 T1 ATE466080 T1 AT E466080T1 AT 04814250 T AT04814250 T AT 04814250T AT 04814250 T AT04814250 T AT 04814250T AT E466080 T1 ATE466080 T1 AT E466080T1
Authority
AT
Austria
Prior art keywords
methods
formulations
denaturing proteins
denaturing
proteins
Prior art date
Application number
AT04814250T
Other languages
English (en)
Inventor
Kim Paulsen
Original Assignee
Qiagen North American Holdings
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen North American Holdings filed Critical Qiagen North American Holdings
Application granted granted Critical
Publication of ATE466080T1 publication Critical patent/ATE466080T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/99—Enzyme inactivation by chemical treatment
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
    • Y10T436/255—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] including use of a solid sorbent, semipermeable membrane, or liquid extraction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Saccharide Compounds (AREA)
AT04814250T 2003-12-16 2004-12-15 Formulierungen und verfahren zum denaturieren von proteinen ATE466080T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US53039203P 2003-12-16 2003-12-16
PCT/US2004/042044 WO2005058933A1 (en) 2003-12-16 2004-12-15 Formulations and methods for denaturing proteins

Publications (1)

Publication Number Publication Date
ATE466080T1 true ATE466080T1 (de) 2010-05-15

Family

ID=34700134

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04814250T ATE466080T1 (de) 2003-12-16 2004-12-15 Formulierungen und verfahren zum denaturieren von proteinen

Country Status (7)

Country Link
US (1) US7115719B2 (de)
EP (1) EP1694692B1 (de)
JP (1) JP4435787B2 (de)
AT (1) ATE466080T1 (de)
CA (1) CA2549985C (de)
DE (1) DE602004026912D1 (de)
WO (1) WO2005058933A1 (de)

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US7569342B2 (en) 1997-12-10 2009-08-04 Sierra Molecular Corp. Removal of molecular assay interferences
US7893228B2 (en) * 2001-10-12 2011-02-22 Qiagen North American Holdings, Inc. Compositions and methods for using a solid support to purify RNA
US7148343B2 (en) * 2001-10-12 2006-12-12 Gentra Systems, Inc. Compositions and methods for using a solid support to purify RNA
US20050032105A1 (en) * 2001-10-12 2005-02-10 Bair Robert Jackson Compositions and methods for using a solid support to purify DNA
EP1727900A4 (de) * 2004-03-26 2008-05-28 Fujifilm Corp Verfahren zur selektiven trennung und reinigungvon rna sowie verfahren zur trennung und reinigung von nukleinsäure
KR20130143677A (ko) * 2004-11-05 2013-12-31 퀴아젠 노쓰 아메리칸 홀딩즈, 인크. 안정화 시약으로부터 핵산을 정제하기 위한 조성물 및 방법
US20070092403A1 (en) * 2005-10-21 2007-04-26 Alan Wirbisky Compact apparatus, compositions and methods for purifying nucleic acids
DE102005059217B4 (de) * 2005-12-07 2011-03-17 Aj Innuscreen Gmbh Verfahren und Testkit zur Trennung, Aufreinigung und Wiedergewinnung von lang- und kurzkettigen Nukleinsäuren
JP4825528B2 (ja) * 2006-02-03 2011-11-30 富士フイルム株式会社 核酸の分離精製方法
EP2024520B1 (de) 2006-05-11 2014-03-05 Becton, Dickinson and Company Verfahren zur proteinextraktion aus zellen
US9207240B2 (en) 2007-11-14 2015-12-08 Arbor Vita Corporation Method of efficient extraction of protein from cells
WO2009121208A1 (zh) * 2008-04-02 2009-10-08 芮宝生医股份有限公司 基于磁性纤维素材料用于分离核酸的试剂、分离促进剂、试剂盒及方法
GB201217405D0 (en) 2012-09-28 2012-11-14 Fermentas Uab Protein removal agent
DK3467108T3 (da) 2013-03-14 2024-06-10 Translate Bio Inc Fremgangsmåder til oprensning af messenger-RNA
US10077439B2 (en) * 2013-03-15 2018-09-18 Modernatx, Inc. Removal of DNA fragments in mRNA production process
WO2014144767A1 (en) * 2013-03-15 2014-09-18 Moderna Therapeutics, Inc. Ion exchange purification of mrna
EP2971033B8 (de) 2013-03-15 2019-07-10 ModernaTX, Inc. Herstellungsverfahren zur herstellung von rna-transkripten
US11377470B2 (en) 2013-03-15 2022-07-05 Modernatx, Inc. Ribonucleic acid purification
ES2750661T3 (es) 2014-04-25 2020-03-26 Translate Bio Inc Métodos para la purificación de ARN mensajero
WO2016180430A1 (en) 2015-05-08 2016-11-17 Curevac Ag Method for producing rna
DE202016009003U1 (de) 2015-05-29 2021-05-28 Curevac Real Estate Gmbh Zusammensetzung umfassend in vitro transkribierte RNA erhältlich durch ein Verfahren zur Herstellung und Reinigung von RNA mit mindestens einem Schritt mit einer tangentialen Flussfiltration
KR20210060480A (ko) 2018-08-24 2021-05-26 트랜슬레이트 바이오 인코포레이티드 전령 rna의 정제 방법
CN110229225B (zh) * 2019-06-12 2021-07-20 山东师范大学 一种南极磷虾中富硫和/或富铯蛋白的制备方法

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US5599667A (en) * 1987-03-02 1997-02-04 Gen-Probe Incorporated Polycationic supports and nucleic acid purification separation and hybridization
US4843155A (en) * 1987-11-19 1989-06-27 Piotr Chomczynski Product and process for isolating RNA
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EP0818461B1 (de) * 1996-07-12 2005-09-28 Toyo Boseki Kabushiki Kaisha Verfahren zur Isolierung von Ribonucleinsäuren.
EP1027145A4 (de) * 1997-09-17 2004-08-25 Gentra Systems Inc Vorrichtung und verfahren zur isolierung von nukleinsäure
US6204375B1 (en) * 1998-07-31 2001-03-20 Ambion, Inc. Methods and reagents for preserving RNA in cell and tissue samples
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US6811788B2 (en) * 2000-01-19 2004-11-02 Baofa Yu Combinations and methods for treating neoplasms
US20050032105A1 (en) * 2001-10-12 2005-02-10 Bair Robert Jackson Compositions and methods for using a solid support to purify DNA
US7893228B2 (en) * 2001-10-12 2011-02-22 Qiagen North American Holdings, Inc. Compositions and methods for using a solid support to purify RNA
US7148343B2 (en) * 2001-10-12 2006-12-12 Gentra Systems, Inc. Compositions and methods for using a solid support to purify RNA
WO2003033739A1 (en) 2001-10-12 2003-04-24 Gentra Systems, Inc. Compositions and methods for using a solid support to purify rna

Also Published As

Publication number Publication date
EP1694692B1 (de) 2010-04-28
WO2005058933A1 (en) 2005-06-30
CA2549985C (en) 2011-05-24
EP1694692A4 (de) 2008-07-23
CA2549985A1 (en) 2005-06-30
JP2007515959A (ja) 2007-06-21
US20050171333A1 (en) 2005-08-04
EP1694692A1 (de) 2006-08-30
US7115719B2 (en) 2006-10-03
JP4435787B2 (ja) 2010-03-24
DE602004026912D1 (de) 2010-06-10

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