[go: up one dir, main page]

ATE430807T1 - Verfahren zur kultivierung von circovirus - Google Patents

Verfahren zur kultivierung von circovirus

Info

Publication number
ATE430807T1
ATE430807T1 AT02712700T AT02712700T ATE430807T1 AT E430807 T1 ATE430807 T1 AT E430807T1 AT 02712700 T AT02712700 T AT 02712700T AT 02712700 T AT02712700 T AT 02712700T AT E430807 T1 ATE430807 T1 AT E430807T1
Authority
AT
Austria
Prior art keywords
circovirus
cultivation
methods
culturing
present
Prior art date
Application number
AT02712700T
Other languages
English (en)
Inventor
Qiang Liu
Suresh Tikoo
Philip Willson
Lorne Babiuk
Original Assignee
Univ Saskatchewan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Saskatchewan filed Critical Univ Saskatchewan
Application granted granted Critical
Publication of ATE430807T1 publication Critical patent/ATE430807T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/10011—Circoviridae
    • C12N2750/10021—Viruses as such, e.g. new isolates, mutants or their genomic sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/10011—Circoviridae
    • C12N2750/10041—Use of virus, viral particle or viral elements as a vector
    • C12N2750/10043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/10011—Circoviridae
    • C12N2750/10051—Methods of production or purification of viral material
    • C12N2750/10052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT02712700T 2001-03-27 2002-03-27 Verfahren zur kultivierung von circovirus ATE430807T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US27917301P 2001-03-27 2001-03-27
PCT/CA2002/000413 WO2002077210A2 (en) 2001-03-27 2002-03-27 Methods to culture circovirus

Publications (1)

Publication Number Publication Date
ATE430807T1 true ATE430807T1 (de) 2009-05-15

Family

ID=23067939

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02712700T ATE430807T1 (de) 2001-03-27 2002-03-27 Verfahren zur kultivierung von circovirus

Country Status (24)

Country Link
US (3) US6794163B2 (de)
EP (1) EP1379671B1 (de)
JP (1) JP4038127B2 (de)
KR (1) KR100876629B1 (de)
CN (2) CN1309836C (de)
AT (1) ATE430807T1 (de)
AU (1) AU2002244584B2 (de)
BR (1) BRPI0208456B8 (de)
CA (1) CA2442135C (de)
CY (1) CY1109267T1 (de)
CZ (1) CZ304525B6 (de)
DE (1) DE60232234D1 (de)
DK (1) DK1379671T3 (de)
ES (1) ES2324466T3 (de)
HU (1) HU228065B1 (de)
MX (1) MXPA03008810A (de)
MY (2) MY148759A (de)
PL (1) PL202951B1 (de)
PT (1) PT1379671E (de)
RU (2) RU2312142C2 (de)
SK (1) SK287969B6 (de)
UA (1) UA82647C2 (de)
WO (1) WO2002077210A2 (de)
ZA (1) ZA200307517B (de)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040062775A1 (en) * 1997-12-05 2004-04-01 Agence Francaise De Securite Sanitaire Des Aliments Circovirus sequences associated with piglet weight loss disease (PWD)
FR2772047B1 (fr) * 1997-12-05 2004-04-09 Ct Nat D Etudes Veterinaires E Sequence genomique et polypeptides de circovirus associe a la maladie de l'amaigrissement du porcelet (map), applications au diagnostic et a la prevention et/ou au traitement de l'infection
ES2170622B1 (es) * 1999-12-03 2004-05-16 Consejo Superior De Investigaciones Cientificas Clones y vectores infectivos derivados de coronavirus y sus aplicaciones.
PT1379671E (pt) 2001-03-27 2009-07-20 Univ Saskatchewan Métodos de cultura de circovírus
US20030096377A1 (en) * 2001-06-28 2003-05-22 Virginia Tech Intellectual Properties, Inc. Differential PCR-RFLP assay for detecting and distinguishing between nonpathogenic PCV-1 and pathogenic PCV-2
US7279166B2 (en) 2001-12-12 2007-10-09 Virginia Tech Intellectual Properties, Inc. Chimeric infectious DNA clones, chimeric porcine circoviruses and uses thereof
US7276353B2 (en) * 2001-12-12 2007-10-02 Virginia Tech Intellectual Properties, Inc. Chimeric infectious DNA clones, chimeric porcine circoviruses and uses thereof
UA95602C2 (ru) 2004-12-30 2011-08-25 Берингер Ингельхейм Ветмедика, Инк. Иммуногенная композиция цвс2 и способы приготовления такой композиции
US7700285B1 (en) 2005-12-29 2010-04-20 Boehringer Ingelheim Vetmedica, Inc. PCV2 immunogenic compositions and methods of producing such compositions
US7833707B2 (en) * 2004-12-30 2010-11-16 Boehringer Ingelheim Vetmedica, Inc. Methods of overexpression and recovery of porcine circovirus type 2 ORF2
US8834891B2 (en) 2005-03-14 2014-09-16 Boehringer Ingelheim Vetmedica, Inc. Immunogenic compositions comprising Lawsonia intracellularis
PT2371383E (pt) * 2005-12-29 2015-11-24 Boehringer Ingelheim Vetmed Utilização de uma composição imunogénica de pcv2 para atenuar os sintomas clínicos em porcos
MX365868B (es) * 2005-12-29 2019-06-18 Boehringer Ingelheim Vetmedica Inc Composiciones inmunogenicas de pcv2 multivalentes y metodos de producir composiciones de este tipo.
EP2859900A1 (de) 2006-12-11 2015-04-15 Boehringer Ingelheim Vetmedica, Inc. Wirksames Verfahren zur Behandlung von Infektionen mit dem porcinen Circovirus und Lawsonia intracellularis
HUE044410T2 (hu) * 2006-12-15 2019-10-28 Boehringer Ingelheim Animal Health Usa Inc Egydózisú anti-PCV2 sertés vakcina
EP1941903A1 (de) 2007-01-03 2008-07-09 Boehringer Ingelheim Vetmedica Gmbh Prophylaxe und Behandlung von PRDC
EP1958644A1 (de) 2007-02-13 2008-08-20 Boehringer Ingelheim Vetmedica Gmbh Vorbeugung und Behandlung von subklinischer PCVD
US8518696B2 (en) * 2007-05-11 2013-08-27 Temasek Life Sciences Laboratory Limited Production of homogeneous cell line highly permissive to porcine circovirus type 2 (PCV2) infection
WO2008151215A1 (en) * 2007-06-04 2008-12-11 Merial Limited Production of porcine circovirus proteins in plants
US7829274B2 (en) * 2007-09-04 2010-11-09 Boehringer Ingelheim Vetmedica, Inc. Reduction of concomitant infections in pigs by the use of PCV2 antigen
KR20100094587A (ko) * 2007-12-21 2010-08-26 와이어쓰 엘엘씨 돼지 써코바이러스에 대해 돼지를 면역시키는 방법 및 조성물
CN101932336B (zh) * 2007-12-31 2014-07-09 贝林格尔.英格海姆维特梅迪卡有限公司 有外来氨基酸插入的pcv2 orf2病毒样颗粒
BRPI0907438A2 (pt) * 2008-01-23 2015-08-04 Boehringer Ingelheim Vetmed Composições imunogênicas para mycoplasma hyopneumoniae e pcv2 e métodos para produzir tais composições
NZ588639A (en) * 2008-04-16 2012-10-26 Virginia Tech Intell Prop CHIMERIC PORCINE CIRCOVIRUS PCV2Gen-1Rep AND USES THEREOF
AR078253A1 (es) * 2009-09-02 2011-10-26 Boehringer Ingelheim Vetmed Metodos para reducir la actividad antivirica en composiciones pcv-2 y composiciones pcv-2 con mejor inmunogenicidad
WO2011091389A2 (en) * 2010-01-25 2011-07-28 Blood Systems, Inc. Cyclovirus and method of use
CA2925281C (en) 2013-09-25 2022-05-03 Zoetis Services Llc Pcv2b divergent vaccine composition and methods of use
ES2778425T3 (es) 2013-10-02 2020-08-10 Boehringer Ingelheim Animal Health Usa Inc Variante de la proteína ORF2 del PCV2 y partículas similares a virus compuestas por esta
EP3294876A1 (de) 2015-05-14 2018-03-21 Merial, Inc. Verfahren zur herstellung von porcinem circovirus und von pcv2-impfstoffen
WO2018071511A1 (en) * 2016-10-11 2018-04-19 Orion Labs Group communication forwarding to a secondary service
CN114934020B (zh) * 2022-01-30 2024-02-13 山东省滨州畜牧兽医研究院 猪视网膜上皮细胞系及其建立方法和应用

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20070108418A (ko) * 1996-10-23 2007-11-09 더 트러스티스 오브 더 유니버시티 오브 펜실바니아 면역 치료법 및 개량 백신
AUPO856097A0 (en) 1997-08-14 1997-09-04 Commonwealth Scientific And Industrial Research Organisation Vector
US6391314B1 (en) 1997-10-03 2002-05-21 Merial Porcine circoviruses vaccines diagnostic reagents
FR2781159B1 (fr) * 1998-07-06 2000-10-06 Merial Sas Vaccin circovirus et parvovirus porcin
US7192594B2 (en) 1997-10-03 2007-03-20 Merial Limited Postweaning multisystemic wasting syndrome and porcine circovirus from pigs
UA78180C2 (uk) 1997-10-03 2007-03-15 Меріаль Кільцевий вірус свині типу ii, вакцини та діагностичні реагенти
US6517843B1 (en) * 1999-08-31 2003-02-11 Merial Reduction of porcine circovirus-2 viral load with inactivated PCV-2
FR2772047B1 (fr) 1997-12-05 2004-04-09 Ct Nat D Etudes Veterinaires E Sequence genomique et polypeptides de circovirus associe a la maladie de l'amaigrissement du porcelet (map), applications au diagnostic et a la prevention et/ou au traitement de l'infection
US6287856B1 (en) 1998-03-13 2001-09-11 University Of Georgia Research Foundation, Inc. Vaccines against circovirus infections
US6492343B1 (en) 1998-04-15 2002-12-10 University Of Saskatchewan Porcine adenovirus type 3 genome
ATE451378T1 (de) 1998-09-08 2009-12-15 Brij P Giri Chemolumineszente 1,2-dioxetane
WO2000026395A2 (en) 1998-11-02 2000-05-11 University Of Saskatchewan Bovine cells expressing adenovirus essential functions for propagation of recombinant adenoviral vectors
FR2789695B1 (fr) 1999-02-11 2003-03-07 Merial Sas Vecteurs et vaccins viraux a base d'adenovirus porcins recombines et replicatifs
US6497883B1 (en) 1999-06-10 2002-12-24 Merial Porcine circovirus recombinant poxvirus vaccine
US6943152B1 (en) 1999-06-10 2005-09-13 Merial DNA vaccine-PCV
CA2413265A1 (en) 2000-05-03 2001-11-08 University Of Guelph Porcine adenovirus type 5 vector and vaccine
PT1379671E (pt) * 2001-03-27 2009-07-20 Univ Saskatchewan Métodos de cultura de circovírus

Also Published As

Publication number Publication date
KR20040002881A (ko) 2004-01-07
WO2002077210A2 (en) 2002-10-03
SK12102003A3 (sk) 2004-05-04
CA2442135C (en) 2008-08-19
RU2007110808A (ru) 2008-09-27
KR100876629B1 (ko) 2009-01-07
US7560278B2 (en) 2009-07-14
MY148759A (en) 2013-05-31
BRPI0208456B8 (pt) 2021-05-25
SK287969B6 (sk) 2012-08-06
WO2002077210A3 (en) 2003-08-21
CN1840656A (zh) 2006-10-04
UA82647C2 (uk) 2008-05-12
CY1109267T1 (el) 2014-07-02
US6794163B2 (en) 2004-09-21
US7172899B2 (en) 2007-02-06
RU2003131342A (ru) 2005-04-20
CZ20032584A3 (en) 2004-03-17
AU2002244584B2 (en) 2006-12-14
EP1379671A2 (de) 2004-01-14
EP1379671B1 (de) 2009-05-06
MXPA03008810A (es) 2004-02-17
RU2312142C2 (ru) 2007-12-10
US20020177216A1 (en) 2002-11-28
HU228065B1 (en) 2012-09-28
CN1840656B (zh) 2010-06-02
ES2324466T3 (es) 2009-08-07
JP2005500822A (ja) 2005-01-13
BR0208456A (pt) 2004-03-02
PT1379671E (pt) 2009-07-20
CA2442135A1 (en) 2002-10-03
CZ304525B6 (cs) 2014-06-18
US20070128700A1 (en) 2007-06-07
CN1309836C (zh) 2007-04-11
HUP0500161A3 (en) 2010-01-28
PL202951B1 (pl) 2009-08-31
DK1379671T3 (da) 2009-06-29
DE60232234D1 (de) 2009-06-18
CN1625598A (zh) 2005-06-08
JP4038127B2 (ja) 2008-01-23
BRPI0208456B1 (pt) 2016-10-18
US20050239187A1 (en) 2005-10-27
HUP0500161A2 (hu) 2005-05-30
ZA200307517B (en) 2004-09-27
PL364077A1 (en) 2004-12-13
MY146518A (en) 2012-08-15

Similar Documents

Publication Publication Date Title
ATE430807T1 (de) Verfahren zur kultivierung von circovirus
DE60031419D1 (de) Zusammensetzungen und verfahren für verbesserte zellkultur
ATE420945T1 (de) Chemisch definiertes medium für kultivierte säugerzellen
DE60144145D1 (de) Subtilisin-variante
ATE422908T1 (de) Zusammensetzung zur geweberegeneration
WO2006127809A3 (en) Methods for expansion and analysis of cultured hematopoietic stem cells
ATE548391T1 (de) Gefangene bindungsproteine als biosensoren
ATE405644T1 (de) Verfahren zur herstellung und reinigung von erythropoietin
ATE407138T1 (de) Verfahren zur herstellung interferierender rna- moleküle in säugetierzellen und therapeutische anwendungen solcher moleküle
DK1650307T3 (da) Fremgangsmåde til fremstilling af rekombinante proteiner ved anvendelse af apoptoseinhibitorer
DE60141510D1 (de) Methode zur verhinderung der transplantatabstossung unter verwendung von tgf-beta zur induktion von t-suppressorzellen
EP1679964A4 (de) Zusammensetzungen mit bzw. verfahren zur verwendung von sirna-amphipathischen verbindungen und polykationen
DE60232322D1 (de) Verfahren
UA92157C2 (ru) Способ продуцирования гормона роста
ATE398169T1 (de) Verfahren zur reinigung von oozysten mittels enzymatischer verdauung von faeces
ATE511397T1 (de) Lungenzellen von baumwollratten zur virenkultur
WO2007005659A3 (en) Methods and compositions for culturing keratinocytes
WO2004016743A3 (en) Culturing anaplasma
DK1458853T3 (da) Fremgangsmåde til donorspecifik krydsmatchning
ATE313622T1 (de) Verfahren zur züchtung von haftenden tierischen zellen
ATE374243T1 (de) Knorpelzell-kulturmedium und verwendung desselben
ATE342665T1 (de) Verfahren zur extraktion und verbesserung der verdaubarkeit von palmaria palmata proteinen
TW200722520A (en) Human mensenchymal stem cells and culturing methods thereof
DE60326157D1 (de) Verfahren zur identifizierung von modulatoren der glucoseaufnahme

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1379671

Country of ref document: EP