[go: up one dir, main page]

DE60144145D1 - Subtilisin-variante - Google Patents

Subtilisin-variante

Info

Publication number
DE60144145D1
DE60144145D1 DE60144145T DE60144145T DE60144145D1 DE 60144145 D1 DE60144145 D1 DE 60144145D1 DE 60144145 T DE60144145 T DE 60144145T DE 60144145 T DE60144145 T DE 60144145T DE 60144145 D1 DE60144145 D1 DE 60144145D1
Authority
DE
Germany
Prior art keywords
homologues
new
subtilisin variant
proteins
subtilisin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60144145T
Other languages
English (en)
Inventor
Jon E Ness
Mark Welch
Lorraine J Giver
Joel R Cherry
Torben V Borchert
Willem P Stemmer
Jeremy Minshull
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Maxygen Inc
Original Assignee
Novozymes AS
Maxygen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes AS, Maxygen Inc filed Critical Novozymes AS
Application granted granted Critical
Publication of DE60144145D1 publication Critical patent/DE60144145D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6402—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals
    • C12N9/6405—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals not being snakes
    • C12N9/6408—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DE60144145T 2000-04-03 2001-04-02 Subtilisin-variante Expired - Lifetime DE60144145D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US19414300P 2000-04-03 2000-04-03
PCT/US2001/010781 WO2001075087A2 (en) 2000-04-03 2001-04-02 Subtilisin variants

Publications (1)

Publication Number Publication Date
DE60144145D1 true DE60144145D1 (de) 2011-04-14

Family

ID=22716459

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60144145T Expired - Lifetime DE60144145D1 (de) 2000-04-03 2001-04-02 Subtilisin-variante

Country Status (8)

Country Link
US (4) US6902922B2 (de)
EP (1) EP1287123B1 (de)
JP (1) JP2004508011A (de)
AT (1) ATE500323T1 (de)
AU (4) AU4981101A (de)
CA (1) CA2405063C (de)
DE (1) DE60144145D1 (de)
WO (1) WO2001075087A2 (de)

Families Citing this family (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
US7153655B2 (en) 1998-06-16 2006-12-26 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence
EP1287123B1 (de) * 2000-04-03 2011-03-02 Maxygen, Inc. Subtilisin-variante
ATE471377T1 (de) * 2000-04-28 2010-07-15 Novozymes As Variante eines lipolytischen enzyms
US7109016B2 (en) * 2000-08-21 2006-09-19 Novozymes A/S Subtilase enzymes
AU758744B2 (en) * 2000-10-31 2003-03-27 F. Hoffmann-La Roche Ag Methods for the analysis of non-proteinaceous components using a protease from a bacillus strain
US6958213B2 (en) 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function
DE10121463A1 (de) * 2001-05-02 2003-02-27 Henkel Kgaa Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
DK200101090A (da) 2001-07-12 2001-08-16 Novozymes As Subtilase variants
DE10162727A1 (de) * 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14391) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE60330346D1 (de) * 2002-01-16 2010-01-14 Genencor Int Mehrfachsubstituierte proteasevarianten
JP5328002B2 (ja) * 2002-01-16 2013-10-30 ジェネンコー・インターナショナル・インク 複数置換プロテアーゼ変異体
DK1504098T4 (da) 2002-05-17 2011-05-23 Alligator Bioscience Ab Fremgangsmåde til molekyulær udvikling in vitro af proteinfunktion
US7888093B2 (en) * 2002-11-06 2011-02-15 Novozymes A/S Subtilase variants
CA2526341C (en) * 2003-05-07 2013-02-19 Novozymes A/S Variant subtilisin enzymes (subtilases)
US9695251B2 (en) 2003-10-31 2017-07-04 The Regents Of The University Of California Activatable cell penetrating peptides with quenched fluorophores
CN1969042B (zh) * 2004-04-02 2014-09-24 诺维信公司 具有改变的免疫原性的枯草杆菌酶变体
US20090060933A1 (en) * 2004-06-14 2009-03-05 Estell David A Proteases producing an altered immunogenic response and methods of making and using the same
JP2009534022A (ja) 2006-04-20 2009-09-24 ノボザイムス アクティーゼルスカブ 卵のシミに対する洗浄能力が向上したサビナーゼ変異体
US7802883B2 (en) 2007-12-20 2010-09-28 Johnson & Johnson Vision Care, Inc. Cosmetic contact lenses having a sparkle effect
CN104130993B (zh) * 2008-06-06 2018-10-23 丹尼斯科美国公司 包括微生物蛋白酶变体的组合物和方法
US20090312196A1 (en) 2008-06-13 2009-12-17 Codexis, Inc. Method of synthesizing polynucleotide variants
WO2009152336A1 (en) 2008-06-13 2009-12-17 Codexis, Inc. Method of synthesizing polynucleotide variants
US20110082055A1 (en) * 2009-09-18 2011-04-07 Codexis, Inc. Reduced codon mutagenesis
DK2478137T3 (da) 2009-09-18 2017-11-27 Codexis Inc Reduceret kodonmutagenese
EP3019602B1 (de) 2013-07-10 2018-09-05 Glykos Finland Oy Filamentöse pilzzellen mit mehrfacher proteasendefizienz und verfahren zur verwendung davon
CN108064266A (zh) 2014-07-21 2018-05-22 格利科斯芬兰公司 在丝状真菌中具有哺乳动物样n-聚糖的糖蛋白的制备
WO2017193022A1 (en) 2016-05-05 2017-11-09 Codexis, Inc. Penicillin-g acylases
EP3878967A3 (de) 2017-02-03 2021-12-08 Codexis, Inc. Manipulierte glycosyltransferasen und verfahren zur steviol-glycosid-glucosylierung
CN110573175A (zh) 2017-02-13 2019-12-13 科德克希思公司 工程化苯丙氨酸氨裂合酶多肽
JP7045725B2 (ja) 2017-04-27 2022-04-01 コデクシス， インコーポレイテッド ケトレダクターゼポリペプチドおよびポリヌクレオチド
KR20200003903A (ko) 2017-05-08 2020-01-10 코덱시스, 인코포레이티드 조작된 리가제 변이체
EP3638688A4 (de) 2017-06-14 2021-07-07 Codexis, Inc. Manipulierte transaminase-polypeptide für industrielle biokatalyse
EP3645713A4 (de) 2017-06-27 2021-06-30 Codexis, Inc. Penicillin-g-acylasen
WO2019094301A1 (en) 2017-11-07 2019-05-16 Codexis, Inc. Transglutaminase variants
US11015180B2 (en) 2017-12-13 2021-05-25 Codexis, Inc. Carboxyesterase polypeptides for amide coupling
US10900055B2 (en) 2018-06-12 2021-01-26 Codexis, Inc. Engineered tyrosine ammonia lyase
CN112673091A (zh) 2018-07-09 2021-04-16 科德克希思公司 工程化泛酸激酶变体酶
CA3103514A1 (en) 2018-07-09 2020-01-16 Codexis, Inc. Engineered galactose oxidase variant enzymes
SG11202012107RA (en) 2018-07-09 2021-01-28 Codexis Inc Engineered deoxyribose-phosphate aldolases
WO2020014047A1 (en) 2018-07-09 2020-01-16 Codexis, Inc. Engineered purine nucleoside phosphorylase variant enzymes
MX2021000322A (es) 2018-07-09 2021-03-25 Codexis Inc Enzimas de variante fosfopentomutasa modificada geneticamente.
CN112672989A (zh) 2018-07-12 2021-04-16 科德克希思公司 工程化苯丙氨酸氨裂合酶多肽
KR20210040408A (ko) 2018-07-30 2021-04-13 코덱시스, 인코포레이티드 조작된 글리코실트랜스퍼라제 및 스테비올 글리코시드 글루코실화 방법
US11060075B2 (en) 2018-10-29 2021-07-13 Codexis, Inc. Engineered DNA polymerase variants
EP3893860A4 (de) 2018-12-14 2023-03-29 Codexis, Inc. Manipulierte tyrosin-ammoniak-lyase
JP7245676B2 (ja) * 2019-03-11 2023-03-24 花王株式会社 変異プロテアーゼ
SG11202110860SA (en) 2019-05-01 2021-10-28 Codexis Inc Engineered glucose dehydrogenases and methods for the reductive amination of ketone and amine compounds
CN114127272A (zh) 2019-05-01 2022-03-01 科德克希思公司 工程化亚胺还原酶和用于酮和胺化合物的还原胺化的方法
EP4501403A2 (de) 2019-08-30 2025-02-05 Société des Produits Nestlé S.A. Manipulierte lipasevarianten
EP4027969A4 (de) 2019-09-12 2023-09-27 Codexis, Inc. Peroxidaseaktivität gegen 10-acetyl-3,7-dihydroxyphenoxazin
CA3165484A1 (en) 2019-12-20 2021-06-24 Codexis, Inc. Engineered acid alpha-glucosidase variants
WO2021158686A1 (en) 2020-02-04 2021-08-12 Codexis, Inc. Engineered leucine decarboxylases
US11788071B2 (en) 2020-04-10 2023-10-17 Codexis, Inc. Engineered transaminase polypeptides
BR112023003606A2 (pt) 2020-08-28 2023-03-28 Codexis Inc Protease recombinante, composição, sequência polinucleotídica recombinante, vetor de expressão, célula hospedeira, métodos para produzir uma protease recombinante e para tratar e/ou prevenir os sintomas de insuficiência pancreática, e, uso
CA3191900A1 (en) 2020-08-28 2022-03-03 Codexis, Inc. Engineered amylase variants
WO2022061210A1 (en) * 2020-09-21 2022-03-24 Bayer Cropscience Lp Novel serine proteases
CA3204825A1 (en) 2020-12-18 2022-06-23 Codexis, Inc. Engineered uridine phosphorylase variant enzymes
IL305928A (en) 2021-04-02 2023-11-01 Codexis Inc Engineered acetate kinase variant enzymes
WO2022212824A1 (en) 2021-04-02 2022-10-06 Codexis, Inc. Engineered adenylate kinase variant enzymes
CA3214973A1 (en) 2021-04-02 2022-10-06 Codexis, Inc. Engineered guanylate kinase variant enzymes
WO2022212832A1 (en) 2021-04-02 2022-10-06 Codexis, Inc. Engineered cyclic gmp-amp synthase (cgas) variant enzymes
TW202313969A (zh) 2021-05-21 2023-04-01 美商克迪科思股份有限公司 經工程化的甲硫胺酸γ解離酶變異體
WO2023064938A1 (en) 2021-10-15 2023-04-20 Codexis, Inc. Engineered dna polymerase variants
CN119630280A (zh) 2022-05-14 2025-03-14 诺维信公司 用于预防、处理、抑制和/或消除植物病原性侵染和感染的组合物和方法

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US49827A (en) * 1865-09-05 Improvement in machines for making cement pipe
AU620026B2 (en) * 1987-02-27 1992-02-13 Genencor International, Inc. Molecular cloning and expression of genes encoding proteolytic enzymes
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
US5858757A (en) * 1991-05-01 1999-01-12 Novo Nordisk A/S Stabilized enzymes and detergent compositions
US5340735A (en) * 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
US5316935A (en) 1992-04-06 1994-05-31 California Institute Of Technology Subtilisin variants suitable for hydrolysis and synthesis in organic media
ATE376061T1 (de) * 1992-07-17 2007-11-15 Genencor Int Hochalkalische serinproteasen
CA2145420C (en) * 1992-09-24 2005-01-04 Stanley E. Mainzer Cleaning compositions containing novel alkaline proteases
WO1995010592A1 (en) * 1993-10-14 1995-04-20 The Procter & Gamble Company Bleaching compositions comprising protease enzymes
EP0723579B1 (de) 1993-10-14 2007-05-02 The Procter & Gamble Company Proteasehaltige reinigungsmittel
US5972042A (en) 1995-12-22 1999-10-26 Novo Nordisk A/S Method for dyeing a material with a dyeing system which contains an enzymatic oxidizing agent
ATE409743T1 (de) * 1996-11-04 2008-10-15 Novozymes As Subtilase varianten und verbindungen
AR015977A1 (es) * 1997-10-23 2001-05-30 Genencor Int Variantes de proteasa multiplemente substituida con carga neta alterada para su empleo en detergentes
US6777218B1 (en) * 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
EP1287123B1 (de) * 2000-04-03 2011-03-02 Maxygen, Inc. Subtilisin-variante
DK200101090A (da) * 2001-07-12 2001-08-16 Novozymes As Subtilase variants
DE10162728A1 (de) * 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14393) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease

Also Published As

Publication number Publication date
AU2006208427A1 (en) 2006-09-28
EP1287123B1 (de) 2011-03-02
AU2009251169A1 (en) 2010-01-21
AU4981101A (en) 2001-10-15
WO2001075087A8 (en) 2001-12-27
WO2001075087A3 (en) 2002-12-19
US20040203130A1 (en) 2004-10-14
JP2004508011A (ja) 2004-03-18
AU2001249811B2 (en) 2006-06-08
AU2006208427B2 (en) 2009-10-01
CA2405063C (en) 2013-06-04
ATE500323T1 (de) 2011-03-15
EP1287123A2 (de) 2003-03-05
CA2405063A1 (en) 2001-10-11
US20100105599A1 (en) 2010-04-29
US20030049827A1 (en) 2003-03-13
US20070270574A1 (en) 2007-11-22
US7220566B2 (en) 2007-05-22
US6902922B2 (en) 2005-06-07
US7605115B2 (en) 2009-10-20
WO2001075087A2 (en) 2001-10-11

Similar Documents

Publication Publication Date Title
DE60144145D1 (de) Subtilisin-variante
WO2002006457A3 (en) Novel lipase genes
DK1210411T3 (da) Sammensætninger og fremgangsmåder til forbedret celledyrkning
DE60127441D1 (de) Dipeptidylpeptidasen
DE69942459D1 (de) Isolierte nukleinsäuremoleküle die t-zellen induzierbare faktoren kodieren (tif), kodierte proteine und ihre anwendung
AR006188A1 (es) "composicion farmaceutica para inhibir la actividad de serinproteasa, metodo in vitro para inhibir la actividad de serinproteasa y metodo para preparar dicha composicion".
DE60037450D1 (de) Funf-helix protein
WO2002031134A3 (en) Novel serine protease genes related to dppiv
ATE400656T1 (de) Mini-dystrophin nukleinsäure- und peptidsequenzen
DE50112802D1 (de) Peptide, deren herstellung und verwendung zur bindung von immunglobulinen
DK0981630T3 (da) Polypeptider med prolyldipeptidylaminopeptidaseaktivitet og nukleinsyrer, der koder for samme
ATE474926T1 (de) Verwendung von clya hemolysin fur ausscheidung von fusionprotein
WO2001025438A3 (en) Ifn-alpha homologues
ATE384787T1 (de) Tripeptidyl-aminopeptidase
ATE439441T1 (de) Veränderte, fluoreszierende proteine
DK1082423T3 (da) Cyclin E2-gener og -proteiner
WO2001064912A3 (en) Triazine degrading enzymes
DK0939769T3 (da) Vertebrat-Smoothened-proteiner
DE69734586D1 (de) Retinoid-metabolisierendes protein
AU2001238064A1 (en) 18477, a human protein kinase and uses therefor
DE69736779D1 (de) Zusammensetzungen von rekombinanten papillomavirus vakzinen
SE9602822D0 (sv) New receptor
WO2002062964A3 (en) Ap1 amine oxidase variants
WO2001040257A3 (en) Modified il-12 subunits and nucleic acids encoding them
WO2002012541A3 (en) Novel motor proteins and methods for their use