ATE113985T1 - Verfahren zur reinigung von rekombinanten polypeptiden. - Google Patents
Verfahren zur reinigung von rekombinanten polypeptiden.Info
- Publication number
- ATE113985T1 ATE113985T1 AT88903520T AT88903520T ATE113985T1 AT E113985 T1 ATE113985 T1 AT E113985T1 AT 88903520 T AT88903520 T AT 88903520T AT 88903520 T AT88903520 T AT 88903520T AT E113985 T1 ATE113985 T1 AT E113985T1
- Authority
- AT
- Austria
- Prior art keywords
- cleavage site
- purification
- fused
- amino acids
- leader sequence
- Prior art date
Links
- 229920001184 polypeptide Polymers 0.000 title abstract 5
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 5
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 4
- 238000000746 purification Methods 0.000 title abstract 3
- 238000003776 cleavage reaction Methods 0.000 abstract 5
- 230000007017 scission Effects 0.000 abstract 5
- 150000001413 amino acids Chemical class 0.000 abstract 2
- 102000005593 Endopeptidases Human genes 0.000 abstract 1
- 108010059378 Endopeptidases Proteins 0.000 abstract 1
- 108020004511 Recombinant DNA Proteins 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/485—Epidermal growth factor [EGF], i.e. urogastrone
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/585—Calcitonins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Endocrinology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US2848487A | 1987-03-20 | 1987-03-20 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE113985T1 true ATE113985T1 (de) | 1994-11-15 |
Family
ID=21843701
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT88903520T ATE113985T1 (de) | 1987-03-20 | 1988-03-04 | Verfahren zur reinigung von rekombinanten polypeptiden. |
Country Status (7)
| Country | Link |
|---|---|
| EP (2) | EP0621340A1 (de) |
| JP (1) | JPH02500876A (de) |
| AT (1) | ATE113985T1 (de) |
| AU (1) | AU605291B2 (de) |
| CA (1) | CA1339208C (de) |
| DE (1) | DE3852074D1 (de) |
| WO (1) | WO1988007085A1 (de) |
Families Citing this family (69)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6121424A (en) * | 1991-11-25 | 2000-09-19 | Enzon, Inc. | Multivalent antigen-binding proteins |
| US5260203A (en) * | 1986-09-02 | 1993-11-09 | Enzon, Inc. | Single polypeptide chain binding molecules |
| US4946778A (en) * | 1987-09-21 | 1990-08-07 | Genex Corporation | Single polypeptide chain binding molecules |
| US5869620A (en) * | 1986-09-02 | 1999-02-09 | Enzon, Inc. | Multivalent antigen-binding proteins |
| EP0305481B1 (de) * | 1987-03-20 | 1993-09-29 | Creative Biomolecules, Inc. | Leader-anteile zur produktion von rekombinanten proteinen |
| DE68915841T2 (de) * | 1988-03-30 | 1995-02-02 | Hitachi Chemical Co Ltd | Verfahren zur Herstellung von Epidermal-Wachstumsfaktor durch genetische Transformation. |
| US5262322A (en) * | 1988-06-24 | 1993-11-16 | Genentech, Inc. | Host transformed with yeast gene and ubiquitin/polypeptide fusions |
| US5108919A (en) * | 1988-06-24 | 1992-04-28 | Genentech, Inc. | Dna sequences encoding yeast ubiquitin hydrolase |
| US5156968A (en) * | 1988-06-24 | 1992-10-20 | Genentech, Inc. | Purified yeast ubiquitin hydrolase |
| EP0470976B1 (de) * | 1989-05-04 | 1993-03-31 | Genentech, Inc. | Verfahren und zusammensetzungen zur isolation von menschlichem relaxin |
| US5595887A (en) * | 1990-07-16 | 1997-01-21 | Bionebraska, Inc. | Purification directed cloning of peptides using carbonic anhydrase as the affinity binding segment |
| DE4105480A1 (de) * | 1991-02-21 | 1992-08-27 | Boehringer Mannheim Gmbh | Verbesserte aktivierung von rekombinanten proteinen |
| US5837491A (en) * | 1991-11-04 | 1998-11-17 | Xoma Corporation | Polynucleotides encoding gelonin sequences |
| US6146850A (en) * | 1991-11-04 | 2000-11-14 | Xoma Corporation | Proteins encoding gelonin sequences |
| US5621083A (en) * | 1991-11-04 | 1997-04-15 | Xoma Corporation | Immunotoxins comprising ribosome-inactivating proteins |
| US5656456A (en) * | 1992-07-13 | 1997-08-12 | Bionebraska, Inc. | Chemical method for selective modification of the N- and/or C-terminal amino acid α-carbon reactive group of a recombinant polypeptide or a portion thereof |
| AU673190B2 (en) * | 1992-07-13 | 1996-10-31 | Bionebraska, Inc. | Method for modification of recombinant polypeptides |
| WO1994012520A1 (en) * | 1992-11-20 | 1994-06-09 | Enzon, Inc. | Linker for linked fusion polypeptides |
| CA2206848A1 (en) * | 1994-12-07 | 1996-06-13 | Bionebraska, Inc. | Production of peptides using recombinant fusion protein constructs |
| AU4415796A (en) * | 1994-12-07 | 1996-06-26 | Bionebraska, Inc. | Production of c-terminal amidated peptides from recombinant protein constructs |
| GB9716790D0 (en) | 1997-08-07 | 1997-10-15 | Creative Peptides Sweden Ab | Recombinant DNA molecules comprising multimeric copies of a gene sequence and expression thereof |
| KR100620554B1 (ko) | 2004-06-05 | 2006-09-06 | 한국생명공학연구원 | Ｔａｇ-72에 대한 인간화 항체 |
| US7951559B2 (en) | 2007-07-25 | 2011-05-31 | E.I. Du Pont De Nemours And Company | Recombinant peptide production using a cross-linkable solubility tag |
| KR101338517B1 (ko) | 2011-04-18 | 2013-12-10 | 연세대학교 산학협력단 | 인간 간-카르복실에스터라제 １을 특이적으로 인식하는 단일클론 항체, 상기 항체를 생산하는 하이브리도마 세포주 및 이의 용도 |
| CN105636976A (zh) | 2012-07-20 | 2016-06-01 | 庆熙大学校产学协力团 | 新型肽标签以及其用途 |
| CN104812773B (zh) | 2012-07-20 | 2018-04-06 | 庆熙大学校产学协力团 | 表位标记用抗体、杂交瘤细胞株以及其用途 |
| KR101453462B1 (ko) | 2013-05-16 | 2014-10-23 | 앱클론(주) | Her2에 특이적으로 결합하는 항체 |
| KR101605421B1 (ko) | 2014-03-05 | 2016-03-23 | 국립암센터 | B 세포 림프종 세포를 특이적으로 인지하는 단일클론항체 및 이의 용도 |
| AU2015355731B2 (en) | 2014-12-03 | 2018-07-26 | Aimed Bio Inc. | Antibody binding to neuropilin 1 and use thereof |
| KR102462811B1 (ko) | 2015-04-27 | 2022-11-04 | 에이비온 주식회사 | 항체에 덴드론이 접합된 면역접합체 및 이의 용도 |
| KR101896882B1 (ko) | 2015-11-30 | 2018-09-11 | 앱클론(주) | Vegfr2에 특이적으로 결합하는 항체 |
| CA3013584C (en) | 2016-02-15 | 2021-01-12 | Samsung Life Public Welfare Foundation | Antibody against egfrviii and use thereof |
| BR112018074275A2 (pt) | 2016-05-27 | 2019-10-01 | Orum Therapeutics Inc | anticorpo de penetração no citosol e método de produção do mesmo |
| WO2017209553A2 (ko) | 2016-06-03 | 2017-12-07 | 사회복지법인 삼성생명공익재단 | 환자 유래 세포를 이용한 항체 스크리닝 방법 |
| WO2017209554A2 (ko) | 2016-06-03 | 2017-12-07 | 사회복지법인 삼성생명공익재단 | 항-nrp1 항체 스크리닝 방법 |
| SG11201810775SA (en) | 2016-06-03 | 2019-01-30 | Samsung Life Public Welfare Foundation | Anti-nrp1 antibody screening method |
| CA3026236C (en) | 2016-06-03 | 2021-01-26 | Samsung Life Public Welfare Foundation | Method for screening antibody using patient-derived tumor spheroids |
| MY189412A (en) | 2016-08-05 | 2022-02-10 | Y Biologics Inc | Antibody to programmed cell death 1 (pd-1) and use thereof |
| KR102048477B1 (ko) | 2016-08-05 | 2019-11-26 | 주식회사 와이바이오로직스 | 프로그램화된 세포 사멸 단백질 리간드-1 (pd-l1)에 대한 항체 및 이의 용도 |
| WO2018026249A1 (ko) | 2016-08-05 | 2018-02-08 | 주식회사 와이바이오로직스 | 프로그램화된 세포 사멸 단백질 리간드-1 (pd-l1)에 대한 항체 및 이의 용도 |
| KR101757346B1 (ko) | 2017-03-27 | 2017-07-26 | 아주대학교산학협력단 | 항-emap ii 항체 및 이의 용도 |
| KR101970327B1 (ko) | 2017-04-13 | 2019-04-18 | 고려대학교 산학협력단 | 검출 신호의 자가 증폭 원리를 이용한 정확, 신속, 편리한 단일 단계 질병 진단 방법 |
| JP7325339B2 (ja) | 2017-05-30 | 2023-08-14 | チョン クン ダン ファーマシューティカル コーポレーション | 新規な抗ｃ－Ｍｅｔ抗体およびその用途 |
| EP3683236A4 (de) | 2017-09-08 | 2021-10-13 | Y-Biologics Inc. | Antikörper gegen humanes dlk1 und seine verwendung |
| KR102281405B1 (ko) | 2017-10-20 | 2021-07-26 | 주식회사 파멥신 | 항-vista 항체 및 이의 용도 |
| KR102311838B1 (ko) | 2017-12-27 | 2021-10-14 | 주식회사 파멥신 | 항-pd-l1 항체 및 이의 용도 |
| KR102275930B1 (ko) | 2018-03-14 | 2021-07-12 | (주)알테오젠 | Folr1에 특이적으로 결합하는 항체 및 그의 용도 |
| US11945878B2 (en) | 2018-04-18 | 2024-04-02 | Abclon Inc. | Switch molecule and switchable chimeric antigen receptor |
| JP7062134B2 (ja) | 2018-04-27 | 2022-05-16 | アイメッド バイオ インコーポレーテッド | 細胞の磁性ビーズ付着を用いた磁性基盤バイオパンニング方法 |
| KR102115300B1 (ko) | 2018-06-01 | 2020-05-26 | 재단법인 목암생명과학연구소 | 항체 라이브러리 및 이를 이용한 항체 스크리닝 방법 |
| MX2021001672A (es) | 2018-08-10 | 2021-07-15 | Eutilex Co Ltd | Receptor de antigeno quimerico que se une a hla-dr y linfocito t-car. |
| KR102353568B1 (ko) | 2018-11-14 | 2022-01-20 | 주식회사 헬릭스미스 | 안정성이 향상된 항 c-Met 항체 또는 그의 항원 결합 단편 |
| BR112021017303A2 (pt) | 2019-03-06 | 2021-11-16 | Legochem Biosciences Inc | Conjugado de anticorpo ou um sal ou solvato farmaceuticamente aceitável deste, anticorpo que se liga com especificidade ao dlk1 ou um fragmento de ligação ao antígeno deste, composição farmacêutica para prevenção ou tratamento de doença proliferativa, câncer ou angiogênica, métodos para prevenção e para tratamento de doença proliferativa, câncer ou angiogênica, e, uso do conjugado |
| KR102502287B1 (ko) | 2020-04-17 | 2023-02-22 | 앱클론(주) | 항-her2 어피바디 및 이를 스위치 분자로 이용하는 스위처블 키메라 항원 수용체 |
| US20230212286A1 (en) | 2020-06-01 | 2023-07-06 | Innobation Bio Co., Ltd. | Antibodies specific for cd22 and uses thereof |
| EP4163298A4 (de) | 2020-06-09 | 2024-06-12 | Kookmin University Industry Academy Cooperation Foundation | Antikörper mit spezifischer bindung an grp94 oder antigenbindendes fragment davon und verwendungen davon |
| KR102757518B1 (ko) | 2020-12-07 | 2025-01-21 | (주)이노베이션바이오 | Cd47에 특이적인 항체 및 이의 용도 |
| US20240141061A1 (en) | 2021-01-12 | 2024-05-02 | Sg Medical Inc | Novel antibody against cd55 and use thereof |
| JP7651145B2 (ja) | 2021-04-20 | 2025-03-26 | コリア ユニバーシティ リサーチ アンド ビジネス ファウンデーション | 向上した癌細胞死滅効能を有する非対称抗体 |
| US20250129155A1 (en) | 2021-06-30 | 2025-04-24 | Innobation Bio Co., Ltd. | Humanized antibody specific for cd47 and pharmaceutical composition for preventing or treating cd47-related diseases comprising the same |
| US20250019430A1 (en) | 2021-11-05 | 2025-01-16 | Seoul National University Hospital | Resistin-specific antibody and use thereof |
| KR102530111B1 (ko) | 2022-03-11 | 2023-05-10 | 앱클론(주) | 코티닌을 표적하는 항체 또는 그의 항원 결합 단편, 이를 포함하는 키메라 항원 수용체 및 이들의 용도 |
| EP4501964A1 (de) | 2022-03-25 | 2025-02-05 | HK inno.N Corporation | Hla-g-spezifischer antikörper und verwendung davon |
| KR20240008997A (ko) | 2022-07-12 | 2024-01-22 | (재) 스크립스코리아항체연구원 | 사스-코로나 바이러스 2 중화 항체 |
| EP4592312A1 (de) | 2022-09-21 | 2025-07-30 | Industry-Academic Cooperation Foundation, Yonsei University | Neuartiger antikörper zur prävention oder behandlung von fibrotischer erkrankung |
| KR20240114678A (ko) | 2023-01-16 | 2024-07-24 | 주식회사 드노보 바이오테라퓨틱스 | 신규한 항-NKp46 항체 및 이의 용도 |
| KR20240164748A (ko) | 2023-05-12 | 2024-11-20 | 앱클론(주) | 항체 또는 그의 항원 결합 단편, 및 어피바디를 포함하는 이중 항원 표적 단백질 복합체 |
| KR20250091231A (ko) | 2023-05-19 | 2025-06-20 | 서울대학교산학협력단 | 항cd3 항체의 활성화된 t세포의 선택적 제거 용도 |
| KR20240170605A (ko) | 2023-05-23 | 2024-12-04 | 연세대학교 산학협력단 | 신규한 인간 인터류킨-4 수용체 결합 나노바디 및 이를 포함하는 비강 분무 제형 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1200773A (en) * | 1980-02-29 | 1986-02-18 | William J. Rutter | Expression linkers |
| WO1983000346A1 (en) * | 1981-07-15 | 1983-02-03 | Roger Kingdon Craig | Human calcitonin precursor polyprotein structural gene |
| WO1983004030A1 (en) * | 1982-05-06 | 1983-11-24 | Applied Molecular Genetics, Inc. | The manufacture and expression of genes for urogastrone and polypeptide analogs thereof |
| EP0207044A1 (de) * | 1985-06-20 | 1986-12-30 | Monsanto Company | Freisetzung von Peptiden aus Polypeptiden |
-
1988
- 1988-03-04 EP EP94200625A patent/EP0621340A1/de not_active Withdrawn
- 1988-03-04 JP JP63503161A patent/JPH02500876A/ja active Pending
- 1988-03-04 WO PCT/US1988/000717 patent/WO1988007085A1/en not_active Ceased
- 1988-03-04 AU AU15750/88A patent/AU605291B2/en not_active Expired
- 1988-03-04 DE DE3852074T patent/DE3852074D1/de not_active Expired - Lifetime
- 1988-03-04 EP EP88903520A patent/EP0305500B1/de not_active Expired - Lifetime
- 1988-03-04 AT AT88903520T patent/ATE113985T1/de active
- 1988-03-18 CA CA000561913A patent/CA1339208C/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP0621340A1 (de) | 1994-10-26 |
| WO1988007085A1 (en) | 1988-09-22 |
| EP0305500A1 (de) | 1989-03-08 |
| AU605291B2 (en) | 1991-01-10 |
| CA1339208C (en) | 1997-08-05 |
| JPH02500876A (ja) | 1990-03-29 |
| AU1575088A (en) | 1988-10-10 |
| DE3852074D1 (de) | 1994-12-15 |
| EP0305500B1 (de) | 1994-11-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ATE113985T1 (de) | Verfahren zur reinigung von rekombinanten polypeptiden. | |
| KR920701452A (ko) | 프라세싱 부위에 인접한 음전하를 띤 아미노산을 함유하는 이스트 프라세싱 시스템 | |
| DK161681A (da) | Fremgangsmaade til syntese i en bakteriel vaert af et udvalgt modent protein eller polypeptid | |
| ATE124456T1 (de) | Verfahren zur enzymatischen spaltung rekombinanter proteine unter verwendung von iga- proteasen. | |
| GB8314362D0 (en) | Polypeptide and protein products | |
| EP0722461A4 (de) | Expression eines fusionspolypeptides, das ohne leadersequenz aus dem cytoplasma transportiert wird | |
| DK38189A (da) | Hidtil ukendte fusionsproteiner indeholdende glutation-s-transferase | |
| DE3586333D1 (de) | Expressionsvektoren fuer hirudin, transformierte zellen und verfahren zur herstellung von hirudin. | |
| ATE123520T1 (de) | Kex2-endoprotease und verfahren zu ihrer herstellung. | |
| ES2000278A6 (es) | Procedimiento para preparar una proteina de fusion y para preparar una proteina eucariotica. | |
| SE8204811L (sv) | Dna-fragment innefattande en signalsekvens | |
| EA200000202A1 (ru) | Рекомбинантная экспрессия c-пептида инсулина | |
| DE3877035D1 (de) | Gentechnologisches verfahren zur herstellung von polypeptiden. | |
| ATE21118T1 (de) | Verfahren zur umwandlung von praeproinsulinanaloga zu insulinen. | |
| ES2078229T3 (es) | Procedimiento para la preparacion de un polipeptido similar a la motilina y su expresion. | |
| ATE78873T1 (de) | Verfahren zur selektiven spaltung von fusionsproteinen. | |
| ATE103983T1 (de) | Licht-induzierbarer promotor. | |
| DE68915204D1 (de) | Biosynthetisches Verfahren zur Herstellung von chemischen Verbindungen. | |
| ATE191007T1 (de) | Verfahren zur rekombinanten herstellung von proteinen in der hefe hansenula | |
| FI870501A0 (fi) | Menetelmä melanoomaan liittyvän p97-antigeenin antigeenisen sukulaispeptidin tai -proteiinin valmistamiseksi, sekä siinä käyttökelpoiset yhdistelmävirukset, yhdistelmävektorit ja isäntäsolut | |
| ATE192780T1 (de) | Verfahren zur rekombinanten herstellung von proteinen in hefe | |
| ATE139538T1 (de) | Verfahren zur selektiven spaltung von fusionsproteinen |