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AR093330A1 - Metodo para la remocion de adn - Google Patents

Metodo para la remocion de adn

Info

Publication number
AR093330A1
AR093330A1 ARP130104004A ARP130104004A AR093330A1 AR 093330 A1 AR093330 A1 AR 093330A1 AR P130104004 A ARP130104004 A AR P130104004A AR P130104004 A ARP130104004 A AR P130104004A AR 093330 A1 AR093330 A1 AR 093330A1
Authority
AR
Argentina
Prior art keywords
fermentation broth
dna removal
interest
protein
microorganism
Prior art date
Application number
ARP130104004A
Other languages
English (en)
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=47172448&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AR093330(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Novozymes As filed Critical Novozymes As
Publication of AR093330A1 publication Critical patent/AR093330A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Un método para la remoción del ADN residual de un caldo de fermentación que comprende una proteína de interés y un microorganismo que produce la proteína de interés, comprendiendo dicho método: a) agregar un cloruro de polialuminio al caldo de fermentación, y b) separar el microorganismo floculado del caldo de fermentación.
ARP130104004A 2012-11-01 2013-11-01 Metodo para la remocion de adn AR093330A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP12190937 2012-11-01

Publications (1)

Publication Number Publication Date
AR093330A1 true AR093330A1 (es) 2015-06-03

Family

ID=47172448

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP130104004A AR093330A1 (es) 2012-11-01 2013-11-01 Metodo para la remocion de adn

Country Status (6)

Country Link
US (2) US20150291656A1 (es)
EP (1) EP2914611B1 (es)
CN (1) CN104797590A (es)
AR (1) AR093330A1 (es)
DK (1) DK2914611T3 (es)
WO (1) WO2014068083A1 (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2902799B1 (fr) 2006-06-27 2012-10-26 Millipore Corp Procede et unite de preparation d'un echantillon pour l'analyse microbiologique d'un liquide
US8569464B2 (en) 2006-12-21 2013-10-29 Emd Millipore Corporation Purification of proteins
US8362217B2 (en) 2006-12-21 2013-01-29 Emd Millipore Corporation Purification of proteins
WO2008079280A1 (en) 2006-12-21 2008-07-03 Millipore Corporation Purification of proteins
US20100190963A1 (en) 2008-12-16 2010-07-29 Millipore Corporation Stirred Tank Reactor And Method
KR101827855B1 (ko) 2010-05-17 2018-02-12 이엠디 밀리포어 코포레이션 생체분자 정제용 자극 반응성 중합체
CN112639119A (zh) * 2018-08-30 2021-04-09 葛兰素史密斯克莱知识产权发展有限公司 检测核酸的方法
EP3926039A1 (en) 2020-06-17 2021-12-22 AB Enzymes GmbH Use of a nuclease for reducing the viscosity and/or preventing an increase in viscosity of a fermentation broth
JP2025500180A (ja) * 2021-12-23 2025-01-09 ノボザイムス アクティーゼルスカブ 微生物発酵産物中の残留ｄｎａの低減
CN114540514A (zh) * 2022-02-16 2022-05-27 湖州申科生物技术有限公司 检测玫瑰孢链霉菌残留dna的引物对、检测试剂及检测方法

Family Cites Families (84)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3795586A (en) 1969-08-11 1974-03-05 Pabst Brewing Co Recovery of enzymes
DK187280A (da) 1980-04-30 1981-10-31 Novo Industri As Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode
DK135983D0 (da) 1983-03-25 1983-03-25 Novo Industri As Maltogen amylaseenzymprodukt og fremgangsmade til dets fremstilling og anvendelse
WO1987000859A1 (en) 1985-08-09 1987-02-12 Gist-Brocades N.V. Novel lipolytic enzymes and their use in detergent compositions
ATE110768T1 (de) 1986-08-29 1994-09-15 Novo Nordisk As Enzymhaltiger reinigungsmittelzusatz.
US5389536A (en) 1986-11-19 1995-02-14 Genencor, Inc. Lipase from Pseudomonas mendocina having cutinase activity
EP0305216B1 (en) 1987-08-28 1995-08-02 Novo Nordisk A/S Recombinant Humicola lipase and process for the production of recombinant humicola lipases
JPS6474992A (en) 1987-09-16 1989-03-20 Fuji Oil Co Ltd Dna sequence, plasmid and production of lipase
DK6488D0 (da) 1988-01-07 1988-01-07 Novo Industri As Enzymer
ATE129523T1 (de) 1988-01-07 1995-11-15 Novo Nordisk As Spezifische protease.
JP3079276B2 (ja) 1988-02-28 2000-08-21 天野製薬株式会社 組換え体ｄｎａ、それを含むシュードモナス属菌及びそれを用いたリパーゼの製造法
US5648263A (en) 1988-03-24 1997-07-15 Novo Nordisk A/S Methods for reducing the harshness of a cotton-containing fabric
EP0406314B1 (en) 1988-03-24 1993-12-01 Novo Nordisk A/S A cellulase preparation
GB8915658D0 (en) 1989-07-07 1989-08-23 Unilever Plc Enzymes,their production and use
ES2144990T3 (es) 1989-08-25 2000-07-01 Henkel Of America Inc Enzima proteolitica alcalina y metodo de produccion.
HU9202203D0 (en) * 1990-01-29 1992-12-28 Yasuyuki Sakurada Apparatus for purifying sewage water
KR100236540B1 (ko) 1990-04-14 2000-01-15 레클로우크스 라우에르 알카리성 바실러스-리파제, 이를 코-딩하는 dna 서열 및 리파제를 생산하는 바실러스 균주
KR100237148B1 (ko) 1990-05-09 2000-01-15 한센 핀 베네드 엔도글루칸아제 효소를 함유하는 셀룰라제 제조물
DK115890D0 (da) 1990-05-09 1990-05-09 Novo Nordisk As Enzym
KR930702514A (ko) 1990-09-13 1993-09-09 안네 제케르 리파제 변체
ATE219136T1 (de) 1991-01-16 2002-06-15 Procter & Gamble Kompakte waschmittelzusammensetzungen mit hochaktiven cellulasen
DK72992D0 (da) 1992-06-01 1992-06-01 Novo Nordisk As Enzym
DK88892D0 (da) 1992-07-06 1992-07-06 Novo Nordisk As Forbindelse
DK91092D0 (es) 1992-07-10 1992-07-10 Novo Nordisk As
DE69333454T2 (de) 1992-10-06 2005-01-20 Novozymes A/S Zellulosevarianten
CA2138519C (en) 1993-04-27 2007-06-12 Jan Metske Van Der Laan New lipase variants for use in detergent applications
DK52393D0 (es) 1993-05-05 1993-05-05 Novo Nordisk As
JP2859520B2 (ja) 1993-08-30 1999-02-17 ノボ ノルディスク アクティーゼルスカブ リパーゼ及びそれを生産する微生物及びリパーゼ製造方法及びリパーゼ含有洗剤組成物
US5817495A (en) 1993-10-13 1998-10-06 Novo Nordisk A/S H2 O2 -stable peroxidase variants
JPH07143883A (ja) 1993-11-24 1995-06-06 Showa Denko Kk リパーゼ遺伝子及び変異体リパーゼ
AU1806795A (en) 1994-02-22 1995-09-04 Novo Nordisk A/S A method of preparing a variant of a lipolytic enzyme
CA2185101A1 (en) 1994-03-08 1995-09-14 Martin Schulein Novel alkaline cellulases
ES2250969T3 (es) 1994-03-29 2006-04-16 Novozymes A/S Amilasa alcalina de bacilo.
US6017866A (en) 1994-05-04 2000-01-25 Genencor International, Inc. Lipases with improved surfactant resistance
WO1995035381A1 (en) 1994-06-20 1995-12-28 Unilever N.V. Modified pseudomonas lipases and their use
WO1996000292A1 (en) 1994-06-23 1996-01-04 Unilever N.V. Modified pseudomonas lipases and their use
WO1996011262A1 (en) 1994-10-06 1996-04-18 Novo Nordisk A/S An enzyme and enzyme preparation with endoglucanase activity
BE1008998A3 (fr) 1994-10-14 1996-10-01 Solvay Lipase, microorganisme la produisant, procede de preparation de cette lipase et utilisations de celle-ci.
JPH10507642A (ja) 1994-10-26 1998-07-28 ノボ ノルディスク アクティーゼルスカブ 脂肪分解活性を有する酵素
MX9705906A (es) 1995-02-03 1997-10-31 Novo Nordisk As Un metodo para diseñar mutantes de alfa-amilasa con propiedades predeterminadas.
JPH08228778A (ja) 1995-02-27 1996-09-10 Showa Denko Kk 新規なリパーゼ遺伝子及びそれを用いたリパーゼの製造方法
JP3360830B2 (ja) 1995-03-17 2003-01-07 ノボザイムス アクティーゼルスカブ 新規なエンドグルカナーゼ
DK0828753T3 (da) 1995-06-02 2003-06-30 Novozymes As A1/Fe-behandling af en proteinopløsning efterfulgt af membranopkoncentrering
DE69633825T2 (de) 1995-07-14 2005-11-10 Novozymes A/S Modifiziertes enzym mit lipolytischer aktivität
DE69632538T2 (de) 1995-08-11 2005-05-19 Novozymes A/S Neuartige lipolytische enzyme
WO1998008940A1 (en) 1996-08-26 1998-03-05 Novo Nordisk A/S A novel endoglucanase
JP3532576B2 (ja) 1996-09-17 2004-05-31 ノボザイムス アクティーゼルスカブ セルラーゼ変異体
WO1998015257A1 (en) 1996-10-08 1998-04-16 Novo Nordisk A/S Diaminobenzoic acid derivatives as dye precursors
AU5309698A (en) 1996-12-20 1998-07-17 Novo Nordisk A/S Peniophora phytase
JP4217277B2 (ja) 1997-02-20 2009-01-28 コニンクリーケ デーエスエム ナムローゼ フェンノートシャップ 化学的に明確な培地を用いた工業的規模での有用化合物の発酵生産
WO1999027083A1 (en) 1997-11-24 1999-06-03 Novo Nordisk A/S PECTIN DEGRADING ENZYMES FROM $i(BACILLUS LICHENIFORMIS)
US6124127A (en) 1997-11-24 2000-09-26 Novo Nordisk A/S Pectate lyase
EP1032658B1 (en) 1997-11-24 2012-06-27 Novozymes A/S Pectate lyases
EP1058724B1 (en) 1998-02-27 2010-12-01 Novozymes A/S Maltogenic alpha-amylase variants
DK1080044T3 (da) * 1998-04-20 2007-12-03 Calgon Corp Uorganisk præparat, fremgangsmåde til fremstilling og fremgangsmåde til anvendelse
AU1503800A (en) 1998-12-04 2000-06-26 Novozymes A/S Cutinase variants
JP2002534976A (ja) 1999-01-22 2002-10-22 ノボザイムス アクティーゼルスカブ 改良フィターゼ
DK1149160T3 (da) * 1999-01-25 2006-08-14 Novozymes As Genvinding af et protein ved höjt pH
EP1171581A1 (en) 1999-03-31 2002-01-16 Novozymes A/S Lipase variant
CA2408406C (en) 2000-06-02 2014-07-29 Novozymes A/S Cutinase variants
EP1305408B1 (en) 2000-07-19 2009-01-21 Novozymes A/S Cell-wall degrading enzyme variants
WO2002092741A2 (en) 2001-05-14 2002-11-21 Novozymes A/S 0etergent compositions comprising bacillus subtilis pectate lyases
AU2002349300A1 (en) 2001-12-11 2003-06-23 Novozymes A/S Process for harvesting crystalline particles from fermentation broth
JP4426307B2 (ja) 2002-02-08 2010-03-03 ノボザイムス アクティーゼルスカブ フィターゼ変異体
US7601529B2 (en) 2002-05-14 2009-10-13 Novozymes A/S Pectate lyase variants
CN1665924A (zh) 2002-07-01 2005-09-07 诺维信公司 水解氮源
TWI265911B (en) * 2003-01-22 2006-11-11 Dev Center Biotechnology Novel biological flocculants and production methods
CN1902321A (zh) 2003-10-31 2007-01-24 诺维信公司 絮凝包含真菌的发酵液的方法
WO2005056782A2 (en) 2003-12-03 2005-06-23 Genencor International, Inc. Perhydrolase
US7459548B2 (en) * 2004-05-21 2008-12-02 Mo Bio Laboratories, Inc. Kits and processes for removing contaminants from nucleic acids in environmental and biological samples
MXPA06013239A (es) 2004-05-24 2007-02-28 Novozymes As Enzimas para uso farmaceutico.
JP2006129861A (ja) * 2004-10-06 2006-05-25 Japan Science & Technology Agency 閉環状ｄｎａを簡易に調製する方法、キット及び装置
NZ563693A (en) 2005-06-24 2010-08-27 Novozymes As Lipases derived from Thermocyces lanuginosus optionally in combination with a protease and/or amylase for pharmaceutical use
US8518675B2 (en) 2005-12-13 2013-08-27 E. I. Du Pont De Nemours And Company Production of peracids using an enzyme having perhydrolysis activity
ES2629332T3 (es) 2006-01-23 2017-08-08 Novozymes A/S Variantes de lipasa
AR070490A1 (es) 2008-02-29 2010-04-07 Novozymes As Polipeptidos de thermomyces lanuginosus con actividad de lipasa y polinucleotidos que los codifican
EP2367923A2 (en) 2008-12-01 2011-09-28 Danisco US Inc. Enzymes with lipase activity
US20120028318A1 (en) 2009-03-18 2012-02-02 Danisco Us Inc. Fungal cutinase from magnaporthe grisea
CN102361972A (zh) 2009-03-23 2012-02-22 丹尼斯科美国公司 Cal a相关的酰基转移酶及其使用方法
BR112012017056A2 (pt) 2009-12-21 2016-11-22 Danisco Us Inc "composições detergentes contendo lipase de bacillus subtilis e métodos para uso das mesmas"
CN102712880A (zh) 2009-12-21 2012-10-03 丹尼斯科美国公司 含有嗜热脂肪地芽孢杆菌脂肪酶的洗涤剂组合物及其使用方法
CA2783972A1 (en) 2009-12-21 2011-07-14 Christian Adams Detergent compositions containing thermobifida fusca lipase and methods of use thereof
WO2011150157A2 (en) 2010-05-28 2011-12-01 Danisco Us Inc. Detergent compositions containing streptomyces griseus lipase and methods of use thereof
US20140031272A1 (en) 2011-04-08 2014-01-30 Danisco Us Inc. Compositions

Also Published As

Publication number Publication date
CN104797590A (zh) 2015-07-22
US20150291656A1 (en) 2015-10-15
EP2914611A1 (en) 2015-09-09
DK2914611T3 (en) 2018-12-10
EP2914611B1 (en) 2018-08-29
WO2014068083A1 (en) 2014-05-08
US20180030087A1 (en) 2018-02-01

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