DK1149160T3 - Genvinding af et protein ved höjt pH - Google Patents
Genvinding af et protein ved höjt pHInfo
- Publication number
- DK1149160T3 DK1149160T3 DK00901052T DK00901052T DK1149160T3 DK 1149160 T3 DK1149160 T3 DK 1149160T3 DK 00901052 T DK00901052 T DK 00901052T DK 00901052 T DK00901052 T DK 00901052T DK 1149160 T3 DK1149160 T3 DK 1149160T3
- Authority
- DK
- Denmark
- Prior art keywords
- elevated
- protein
- recovery
- solution
- state
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA199900085 | 1999-01-25 | ||
| DKPA199901491 | 1999-10-18 | ||
| EP00901052A EP1149160B1 (en) | 1999-01-25 | 2000-01-24 | Recovery of a protein at high ph |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK1149160T3 true DK1149160T3 (da) | 2006-08-14 |
Family
ID=26063274
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK00901052T DK1149160T3 (da) | 1999-01-25 | 2000-01-24 | Genvinding af et protein ved höjt pH |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US6316240B1 (da) |
| EP (1) | EP1149160B1 (da) |
| JP (1) | JP2002534975A (da) |
| CN (1) | CN1181197C (da) |
| AT (1) | ATE323759T1 (da) |
| AU (1) | AU2093100A (da) |
| DE (1) | DE60027406T2 (da) |
| DK (1) | DK1149160T3 (da) |
| WO (1) | WO2000043502A1 (da) |
Families Citing this family (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2252287T3 (es) | 2000-07-28 | 2006-05-16 | Henkel Kommanditgesellschaft Auf Aktien | Enzima amilolitico de bacillus sp. a7-7 (dsm 12368) asi com0 agentes de lavado y de limpieza con este nuevo enzima amilolitico. |
| JP5453116B2 (ja) * | 2007-03-15 | 2014-03-26 | ノボザイムス アクティーゼルスカブ | 発酵ブロス中のプロテアーゼ結晶の可溶化 |
| BRPI0914982B1 (pt) * | 2008-06-09 | 2019-09-24 | Danisco Us Inc. | Métodos para produção de uma formulação granular contendo enzima e grãnulo contendo enzima |
| GB0908770D0 (en) | 2009-04-24 | 2009-07-01 | Danisco | Method |
| AR093330A1 (es) * | 2012-11-01 | 2015-06-03 | Novozymes As | Metodo para la remocion de adn |
| DK3137483T3 (da) * | 2014-04-30 | 2019-04-23 | Novozymes As | Fremgangsmåde til reducering af DNA-indholdet i en fermenteringsbouillon |
| RU2018124771A (ru) | 2015-12-09 | 2020-01-13 | Басф Се | Способ очистки белка от ферментационных твердых веществ при десорбирующих условиях |
| EP3606936B1 (en) | 2017-04-03 | 2021-10-20 | Novozymes A/S | Recovery process |
| EP3784779A1 (en) | 2018-04-26 | 2021-03-03 | Basf Se | Lipase enzymes |
| US20210115422A1 (en) | 2018-05-03 | 2021-04-22 | Basf Se | Amylase enzymes |
| CN114096676A (zh) | 2019-02-20 | 2022-02-25 | 巴斯夫欧洲公司 | 用确定成分培养基和镁补料的芽孢杆菌工业发酵工艺 |
| MX2021010109A (es) | 2019-02-20 | 2021-09-21 | Basf Se | Proceso de fermentacion industrial para bacillus mediante el uso de un medio definido y una alimentacion de oligoelementos. |
| US20220162576A1 (en) | 2019-03-25 | 2022-05-26 | Basf Se | Amylase enzymes |
| US20220170001A1 (en) | 2019-03-25 | 2022-06-02 | Basf Se | Amylase Enzymes |
| WO2020193532A1 (en) | 2019-03-25 | 2020-10-01 | Basf Se | Cleaning composition having amylase enzymes |
| CN113993878B (zh) * | 2019-06-13 | 2025-01-03 | 巴斯夫欧洲公司 | 用二价阳离子从发酵液中回收蛋白的方法 |
| WO2021001297A1 (en) | 2019-07-02 | 2021-01-07 | Basf Se | Method for preparing a fermentation medium |
| MX2022000253A (es) | 2019-07-05 | 2022-02-03 | Basf Se | Proceso de fermentacion industrial para celulas microbianas mediante el uso de un precultivo de alimentacion por lotes. |
| JP2022545465A (ja) | 2019-08-22 | 2022-10-27 | ビーエーエスエフ ソシエタス・ヨーロピア | アミラーゼバリアント |
| EP4047088A1 (en) | 2021-02-22 | 2022-08-24 | Basf Se | Amylase variants |
| WO2022175435A1 (en) | 2021-02-22 | 2022-08-25 | Basf Se | Amylase variants |
| CN119630806A (zh) | 2022-08-02 | 2025-03-14 | 巴斯夫欧洲公司 | 使用芽孢杆菌属宿主细胞与盐进料的稳定的蛋白质生产方法 |
| WO2024033135A2 (en) | 2022-08-11 | 2024-02-15 | Basf Se | Amylase variants |
| WO2024033136A1 (en) | 2022-08-11 | 2024-02-15 | Basf Se | Amylase variants |
| WO2024033134A1 (en) | 2022-08-11 | 2024-02-15 | Basf Se | Enzyme compositions comprising protease, mannanase, and/or cellulase |
| WO2024033133A2 (en) | 2022-08-11 | 2024-02-15 | Basf Se | Enzyme compositions comprising an amylase |
| WO2024094735A1 (en) | 2022-11-04 | 2024-05-10 | Basf Se | Polypeptides having protease activity for use in detergent compositions |
| WO2024094733A1 (en) | 2022-11-04 | 2024-05-10 | Basf Se | Polypeptides having protease activity for use in detergent compositions |
| WO2024094732A1 (en) | 2022-11-04 | 2024-05-10 | Basf Se | Polypeptides having protease activity for use in detergent compositions |
| EP4389864A1 (en) | 2022-12-20 | 2024-06-26 | Basf Se | Cutinases |
| WO2025031865A1 (en) | 2023-08-08 | 2025-02-13 | Basf Se | Improved bacillus cell with inactivated metalloprotease |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IE55163B1 (en) * | 1982-06-07 | 1990-06-20 | Celltech Ltd | A process for the preparation of chymosin |
| GB2138004B (en) | 1983-03-25 | 1987-05-13 | Celltech Ltd | A process for the production of a protein |
| US5340926A (en) * | 1983-03-25 | 1994-08-23 | Celltech, Limited | Process for the recovery of recombinantly produced protein from insoluble aggregate |
| CA1267615A (en) * | 1984-08-27 | 1990-04-10 | Dan Hadary | Method for recovering purified growth hormones from genetically engineered microorganisms |
| JPH01222780A (ja) | 1988-03-02 | 1989-09-06 | Tosoh Corp | ウロキナーゼ前駆体様蛋白質のリフォールディング方法 |
| US5064943A (en) * | 1988-12-16 | 1991-11-12 | American Cyanamid Company | Method for solubilization and naturation of somatotropin |
| EP0574050A1 (en) | 1992-05-19 | 1993-12-15 | Gist-Brocades N.V. | Large scale separation and purification of fermentation product |
| BE1009844A3 (fr) | 1995-12-22 | 1997-10-07 | Genencor Int | Procede de recolte d'une proteine. |
-
2000
- 2000-01-24 DK DK00901052T patent/DK1149160T3/da active
- 2000-01-24 WO PCT/DK2000/000029 patent/WO2000043502A1/en active IP Right Grant
- 2000-01-24 JP JP2000594910A patent/JP2002534975A/ja active Pending
- 2000-01-24 AU AU20931/00A patent/AU2093100A/en not_active Abandoned
- 2000-01-24 CN CNB008030650A patent/CN1181197C/zh not_active Expired - Lifetime
- 2000-01-24 AT AT00901052T patent/ATE323759T1/de not_active IP Right Cessation
- 2000-01-24 DE DE60027406T patent/DE60027406T2/de not_active Expired - Lifetime
- 2000-01-24 US US09/489,712 patent/US6316240B1/en not_active Expired - Lifetime
- 2000-01-24 EP EP00901052A patent/EP1149160B1/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JP2002534975A (ja) | 2002-10-22 |
| AU2093100A (en) | 2000-08-07 |
| CN1337999A (zh) | 2002-02-27 |
| US6316240B1 (en) | 2001-11-13 |
| DE60027406D1 (de) | 2006-05-24 |
| CN1181197C (zh) | 2004-12-22 |
| ATE323759T1 (de) | 2006-05-15 |
| EP1149160B1 (en) | 2006-04-19 |
| EP1149160A1 (en) | 2001-10-31 |
| DE60027406T2 (de) | 2006-11-23 |
| WO2000043502A1 (en) | 2000-07-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DK1149160T3 (da) | Genvinding af et protein ved höjt pH | |
| ATE326454T1 (de) | Neue bernsteinsäure derivative als cysteine- protease-inhibitoren | |
| TR200200205T2 (tr) | MEK inhibitörleri kullanılarak kronik ağrının tedavi edilmesi | |
| DE59904100D1 (de) | Neue effektoren von dipeptidylpeptidase iv | |
| ATE372334T1 (de) | Phenylpyrazolderivate als seh-inhibitoren | |
| WO2005025496A3 (en) | Aliphatic pyrazinoylguanidine sodium channel blockers | |
| DK1040108T3 (da) | Orthoanthranilamidderivater som antikoagulanter | |
| ATE250066T1 (de) | Verfahren zur reduktion von oxiranyl-epothilonen zu olefinischen epothilonen | |
| FR2810406B1 (fr) | Nouveaux cryptates de terre rare peu sensibles a l'extinction de fluorescence | |
| DK1200418T3 (da) | Nematodicide trifluorbutener | |
| PL344475A1 (en) | Bridged indenopyrrolocarbazoles | |
| TR199800011T1 (xx) | Benzo$g] kuinolin t�revleri | |
| DK1075285T3 (da) | Anvendelse af et vitalt farvestof til at lette kirurgiske procedurer til katarakt ekstraktion | |
| ATE408624T1 (de) | Verfahren zur reinigung von fsh | |
| BR0005636A (pt) | Método para a produção de 4-carboxiamino-1,2,3,4-tetrahidroquinolina 2-substituìda | |
| DK1154986T3 (da) | Fremgangsmåde til fremstilling af iopamidol | |
| ES2160410T3 (es) | Preparados de complejos de protrombina inmunotolerantes. | |
| ATE432946T1 (de) | Abtrennung von fibrinogen von plasmaproteasen | |
| TR199800543A2 (xx) | Benziloksi ikame edilen, aniliye edilmi� N-heterosiklik bile�ikler. | |
| ATE270275T1 (de) | Verfahren zur herstellung von 2-aryl-3-aryl-5- halogen-pyridinen, verwendbar als cox-2 inhibitoren | |
| DE50115655D1 (de) | Verfahren zur herstellung von unsymmetrischen 4,6-bis(aryloxy)pyrimidin-derivaten | |
| DK0477284T3 (da) | Fremgangsmåder til udvinding af naturligt produceret chymosin | |
| DK1097130T3 (da) | Fremgangsmåde til fremstilling af hydroxymethylthiosmörsyre | |
| DE69923995D1 (de) | Thiazol-derivate als ppar-gamma-ligande | |
| DK1206452T3 (da) | Fremgangsmåde til fremstilling af benzoperhydro-isoindolforbindelser |