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Veterinary  Medicine – Open Journal
A formulation was developed using combination of blended natural essential oils as an anti-Vibrio parahemolyticus causing acute hepatopancreatic necrosis disease (AHPND) candidate. Lavandula latifolia, Pinus sylvestris, Jasminum... more
A formulation was developed using combination of blended natural essential oils as an anti-Vibrio parahemolyticus causing acute hepatopancreatic necrosis disease (AHPND) candidate. Lavandula latifolia, Pinus sylvestris, Jasminum officinale, Citrus limon, Prunus avium, Viola odorata, Gardenia jasminoides, Cocos nucifera, Rosa damascene and Eucalyptus globulus, mixed together to develop as anti-V. parahemolyticus product. The treatment group was fed on essential oil mixed feed whereas control group were fed on the regular feed throughout the experiment. The shrimp of both treatment and control were challenged by immersion method at day 8. The cumulative AHPND-gross sign appearance in positive control reached up to 95% at dpi 10 whereas no gross sign appeared in treatment and in negative control. The cumulative mortality reached up to 46.7% at dpi 10 in positive controls whereas no mortality recorded in treatment and in negative control. The V. parahaemolyticus isolated from the hepatopancreas of infected shrimp matched 100% with the existing AHPND strain. The trial results show that the developed natural herbal formulation has significant effect against AHPND in a controlled condition.
Introduction Trypanosomosis is a bottleneck on the livelihood of farmers in the study area and the Ethiopian government has been using 0.4% Delthamethrine impregnated targets and 1% pour-on to reduce the impact of this disease on food... more
Introduction
Trypanosomosis is a bottleneck on the livelihood of farmers in the study area and the Ethiopian government has been using 0.4% Delthamethrine impregnated targets and 1% pour-on to reduce the impact of this disease on food security. However, there was no information on the effect of this chemical on the prevalence of Trypanosomiasis.
Objective
The aim of the study was to evaluate the effect of Deltamethrine in controlling trypanosomiasis.
Methods
Cross-sectional study was conducted on cattle of two districts of Buno Bedele Zone in April 2020 and 2022. Paired samples “t” test statistic was used to summarize data by using statistical package for the social sciences (SPSS) statistics version 21. The approach followed to complete the study was implementing a pre-intervention phase to know the status of trypanosoma before using a chemical in April 2020 and an intervention phase using deltamethrine chemical on cattle at a rate of 1 mL/10 kg body weight and 0.4% Delthamethrine impregnated targets from October 2021 to March 2022. The post-intervention was done in April 2022 to evaluate the prevalence difference.
Results
Out of 576 cattle during pre-intervention 159 (27.60%) cattle were positive with a mean packed cell volume (PCV) of 20.68%. During post-intervention out of 576 cattle, 27 (4.70%) cattle were positive with a mean PCV of 26.10%. For the study, there was strong evidence at a 95% confidence interval (t=3.035, p< 0.05). Delthamethrine chemical application reduces the prevalence of the trypanosoma parasite by ten estimated mean. Also, there was strong evidence at a 95% confidence interval (t=-84.66, p< 0.05) that Delthamethrine chemical application increased the PCV of cattle by five estimated mean.
Conclusion
In both districts, there was strong evidence that deltamethrin chemical application reduces the prevalence of trypanosome parasites. Deltamethrin chemical application increased cattle’s PCV, which showed that an intervention was encouraging to suppress trypanosomosis and should be continually implemented to keep the intervention difference.
Salmonella is one of the most common and significant human and animal food-borne pathogens that cause salmonellosis, which has high medical and monetary costs. Salmonella has a big impact on public health because it can spread zoonotic... more
Salmonella is one of the most common and significant human and animal food-borne pathogens that cause salmonellosis, which has high medical and monetary costs. Salmonella has a big impact on public health because it can spread zoonotic and foodbornediseases to people. Many domestic and wild animals intestinal tracts serve as the main reservoir for Salmonella, which can result in the direct or indirect contamination of a variety of foods with fecal organisms. Salmonellae are etiological agents of diarrheal and systemic infections. They can be shed in large numbers on the faces of clinical cases and carrier animals, causing contamination of the environment and frequently resulting in subclinical infections. Infection in food animals often leads to contamination of meat, eggs, milk and cheese. The organism can also spread through direct contact with infected humans, animals, or feces contaminated environments. Infected food handlers may also act as a source of contamination for foodstuffs, because of the increasing antibiotic resistance of organisms and companion animals, animals are an important source of Salmonella infection for humans. Salmonella can colonize its hosts by invading, adhering, and bypassing the host’s intestinal defense mechanisms, such as gastric acid, thanks to a variety of virulence markers and determinants, including flagella, capsule, plasmids, adhesion systems, and secretion systems. Diagnosis is based on the isolation of the organism either from tissues collected aseptically at necropsy or from faces, rectal swabs or environmental samples, food products, and feedstuffs; prior or current infection of animals by some agents may also be diagnosed serologically.
Aim The study was conducted between December 2021-June 2022 with an objective to assess the current status of veterinary drugs used in veterinary clinics of Dire Dawa and Haramaya districts using a questionnaire, personal interviews, and... more
Aim
The study was conducted between December 2021-June 2022 with an objective to assess the current status of veterinary drugs used in veterinary clinics of Dire Dawa and Haramaya districts using a questionnaire, personal interviews, and observations of public veterinary clinics.
Method
A total number of 40 public veterinary clinics were selected, purposively selected, and included in the study, and veterinary professionals working in each veterinary clinic were conveniently selected by using a non-probability method. The assessment methods such as descriptive drug use indicators, prescribing practices and other drug information sources and drugs of choice at health facilities were included.
Results
The results obtained were as; a majority of the clinicians (57.5%) were Bachelor of Veterinary Science (BVSc) and above. Nearly half (42.5%) of clinicians used only a verbal prescription for prescribing drugs. Oxytetracycline was available in all clinics and 32(80%) responded as it is their first drug of choice for treatment. Only 50% of the clinicians responded as they administer drugs and treat animals presented to their clinic for a definitive (laboratory investigation) diagnosis. The majority (80%) of respondents use antibacterials for prophylaxis. Major factors considered in drug selection by 92.5% of professionals amongst alternative drugs were its availability. About 98% of respondents use promotion materials or leaflets of different drugs as a source of information.
Conclusion
The study showed that the trend of using prescription papers for prescribing drugs was not enough and prescription papers did not include the relevant information that should be incorporated in drug ordering and dispensing drug prescriptions by and large based on tentative diagnosis and inappropriate drug selection were found to be bottlenecks for the successful drug treatment that leads to drug resistance. Therefore, awareness and training should be provided regularly for prescribers and end users about the proper way of drug use.
Introduction An abscess is the most common swelling observed on the skin surface of the animal body with a collection of pus due to microbial invasion of the dermis and its supporting structures. Case Description In the present case,... more
Introduction
An abscess is the most common swelling observed on the skin surface of the animal body with a collection of pus due to microbial invasion of the dermis and its supporting structures.
Case Description
In the present case, nine-years old male Zebu cattle were presented to the Veterinary Teaching Hospital (VTH) with a history of swelling on the cheek. Clinical findings of the mass revealed that there was well-circumscribed, firm, movable cold, and not painful superficial skin swelling on the body of the mandible and check. Culturing on blood agar and gram staining was conducted and then, Corynebacterium pseudotuberculosis was isolated from the sample. Following clinical and laboratory finding the case was diagnosed as caseous lymphadenitis and En bloc excision of the case was performed under local infiltration of anesthesia.
Conclusion
The suture was removed after 15-days post-intervention and the bull was successfully recovered without any other complications. Therefore, management of well-circumscribed, firm, and movable superficial skin abscess in cattle through en bloc excision along with antibacterial therapy and allowing the skin wound to heal by the first intention should be effective.
Dystocia means difficulty in parturition to the point of needing human intervention and it is a major cause of lamb loss in the flock and may result in great economic loss to the farmers. Two local breed adult ewes of different age and... more
Dystocia means difficulty in parturition to the point of needing human intervention and it is a major cause of lamb loss in the flock and may result in great economic loss to the farmers. Two local breed adult ewes of different age and causes of dystocia were presented to Veterinary Teaching Hospital (VTH) with the history of full-term gestation and three-month gestation in case 1 and 2, respectively. Clinical and physical examination finding revealed that there were weak and unable to stand, and straining in case 1 and anorexia, lethargy, recumbent with attempt to stand, abdominal distension and granting sound in case 2. Live fetus in case 1 and died fetus in case 2 was dictated up on abdominal palpation and ultrasonography findings. Vaginal examination also has undergone and revealed that incomplete dilation of cervix in case 1. Depending on diagnostic finding caesarian section were admitted and a single live fetus and a twin died fetuses were removed from case 1 and 2, respectively under line infiltration anaesthesia in case 1 and inverted “L” blocks in case 2. Following the successful caesarian section the dam of case 1 was died after 18-hours of post-surgery but the dam of case 2 was recovered uneventfully without any complications in period of 15 post-operative days.
Introduction Gumboro is an acute, highly contagious, and immunosuppressive viral disease of young chickens less than 17-weeks. However, in local chicken flocks of Ilubabor, there is no known sero-status of the disease. Methods To... more
Introduction
Gumboro is an acute, highly contagious, and immunosuppressive viral disease of young chickens less than 17-weeks. However, in local chicken flocks of Ilubabor, there is no known sero-status of the disease.
Methods
To address this information gap, a cross-sectional study was conducted in local backyard chicken flocks of three districts of Ilubabor Zone where chick mortality and morbidity were a big problem. The objectives of the study were to estimate the seroprevalence of infectious bursal disease virus (IBDV) and to assess its risk factors.
Result
Out of 418 serum samples tested, 180 were positive and the overall chicken level seroprevalence of the IBDV antibody in the study area was found to be 43.10% (95% CI: 38.40-47.85) and flock-level seroprevalence was 45.63% (73/160) (95%CI: 37.91- 53.34) with almost all test positive flock chickens were seropositive. Multivariable analysis at chicken level showed that the odds of IBDV seroprevalence was significantly high in Metu and Bilo Nopa districts, in purchased, in female and adult chickens. Also, it was high at flock level in chickens mixed with exotic breeds, in flocks having greater than 5 chickens.
Conclusion
This study shows that IBDV is circulating in the chicken population of Ilubabor at a high prevalence level. Therefore, further studies on serotypes and strains of IBDV identification should be carried out to design suitable control and prevention measures.
Infectious bursal disease, also known as Gumboro disease, is a highly contagious and acute viral disease of poultry characterised by the destruction of lymphoid cells. Diagnosis of infectious bursal disease involves consideration of the... more
Infectious bursal disease, also known as Gumboro disease, is a highly contagious and acute viral disease of poultry characterised by the destruction of lymphoid cells. Diagnosis of infectious bursal disease involves consideration of the flocks’ history, clinical signs, and lesions. The objectives of this paper are to highlight various commonly used diagnostic methods for infectious bursal disease and to review advances made in diagnostic methods and vaccination strategies for infectious bursal disease, with special emphasis on the strengths and weaknesses of each of those techniques. Isolation of infectious bursal disease virus followed by its serological assay and histopathological examination of the bursa is regarded as the gold standard method of infectious bursal disease diagnosis. Serological tests such as agar gel, immune diffusion, enzyme-linked immuno sorbent assay, and viral neutralisation tests are commonly used laboratory assays in diagnosing infectious bursal disease viruses. Recently, the most accurate and relatively fast diagnostic method, the molecular technique, is widely used. The molecular diagnostic technique is the simplest and most sensitive of the diagnostic techniques reviewed. The virus causes immunosuppression, so the infected chicken recovers from the acute disease but becomes more susceptible to infections by other pathogens. Therefore, prevention is important and vaccination has become the principal control measure of infectious bursal disease virus infection in chickens. Conventional attenuated live and killed vaccines are the most commonly used vaccines. With the advancement of knowledge and technology, new generation or genetically-engineered vaccines like deoxyribonucleic acid and subunit vaccines have been used. Various vaccination strategies, such as in ovo, at hatch, and post hatch vaccination, are used. Hatchery vaccination is becoming a common practice. Based on this review paper, more affordable and effective infectious bursal disease vaccines that are affordable and readily available must be identified with further cost-benefit analysis.
Aim This study was conducted using the questionnaire method to assess the effect of breed and factors affecting conception rate on artificial insemination in dairy cows in Tullo district, Western Haraghe, Ethiopia. Methods A follow-up... more
Aim
This study was conducted using the questionnaire method to assess the effect of breed and factors affecting conception rate on artificial insemination in dairy cows in Tullo district, Western Haraghe, Ethiopia.
Methods
A follow-up study design was conducted from December 2018 to June 2019 to determine the effect of breed and factors affecting conception rate to artificial insemination in dairy cows, taking breed, age, parity, body condition and timing of insemination as risk factors. The demographic factors were recorded by interviewing the owners. Most of the cows were examined for pregnancy diagnosis by rectal palpation of the genital tract at 60-80-days post-artificial insemination.
Results
Out of 114 artificially inseminated cows/heifers, 59 became pregnant, giving an overall first service conception rate of 51.8%. Although the breed, age, parity and body condition score did not affect the conception rate significantly, the pregnancy rate was better in the cross-breed (62.5%), in cows of 5-7-years of age (54.6%), in cows of parity 2-3 (59.2%) and in cows of good body condition score (57.7%). The conception rate in cows inseminated at 12-18-hours after the onset of estrus was significantly higher (62.3%) than those inseminated after 18-hours (31.2%) and before 12-hours (52.4%) after the onset of estrus. Therefore, cows with good body condition score (BCS) and artificial insemination (AI) service at 12-18-hours after the onset of estrus are the best choice of selection for obtaining the best result in the first service conception rate to AI in dairy cows/heifers.
Conclusion
This study reveals that the conception rate was influenced by the time of AI, so awareness should be given to cattle owners, as they should give AI for their cattle at optimum time within 12-18-hours after onset of heat sign.
Aim This study was conducted by using the protocols of post-mortem examinations of meat (visual inspection) to determine the prevalence of bovine cysticercosis in the cattle slaughtered at Haramaya Municipal Abattoir, Eastern Hararghe,... more
Aim
This study was conducted by using the protocols of post-mortem examinations of meat (visual inspection) to determine the prevalence of bovine cysticercosis in the cattle slaughtered at Haramaya Municipal Abattoir, Eastern Hararghe, Ethiopia and to determine zoonotic significance of taeniasis.
Method
A cross-section study design was conducted from November 2020 to March 2021, based on routine meat inspection on simple randomly selected cattle slaughtered at the abattoir. Visual inspection of all exposed surface was made in all active organs. They are shoulder muscles, hearts, masseters (cheek muscle), diaphragms, tongues and livers. This is followed by incision of all those organs to be examined for Cysticercus bovis cysts.
Results
Twenty-one (21) of the 384 cattle examined utilizing the post-mortem examinations meat inspection methodology were positive for C. bovis, resulting in a prevalence of 5.5 %. The masseter muscle (11.5 %) had the highest prevalence of cysts, followed by the triceps (8.3%), heart (5.8%), liver (4.8 %), and tongue (2.9 %). The sex-based prevalence rates were 10 (3.4%) and 11 (12.1%), respectively. The predominance of bodily condition was found to be good (1.3%), medium (11.1%), and bad (50 %). The prevalence male and female e differed substantially by organ, sex, and bodily condition (p>0.05), but not statistically significant by age of the animals (young 2.8% vs. adult 6.1%) (p>0.05). Eight (20%) of the total 40 interviewees had contracted Taenia saginata infection at least once in their lives. Religion showed a significant difference (p>0.05) (Christian 66.7% and Muslim 6.5%). However, there was no statistically significant difference in meat consumption habits (raw 31.6% vs. cooked 9.5%), sex (male 26.5% vs. female 11.8%), age (young 33.3% vs. adult 17.7%), educational status (illiterate 22.2% vs. elementary 14.3% vs. high school 27.3% vs. college 16.7%) or latrine use (proper users 19.4% and non-proper users 25%).
Conclusion
This study to increasing public awareness of the disease, as well as strict routine meat inspections, should be prioritized in order to decrease the parasite's impact.
Narrow pelvis is characterized as an insufficient maternal bony pelvis that does not allow for fetal transit due to a variety of factors. Animal immaturity is one of these reasons. This communication was written with the goal of... more
Narrow pelvis is characterized as an insufficient maternal bony pelvis that does not allow for fetal transit due to a variety of factors. Animal immaturity is one of these reasons. This communication was written with the goal of documenting surgical management of dystocia and its impact on a two-year-old heifer who was with a full-term pregnancy. A vaginal delivery was tried but failed due to the narrow maternal pelvis. To avoid any post-operative problems, the surgical management was carried out under strict aseptically. A volume of 30 ml 2% Lidocaine Hydrochloride was infiltrated on left flank with inverted L-block techniques. While the cow was on her right lateral recumbency. The uterus was emptied of a deceased fetus but fetal membrane with the intact umbilical cord was returned back to the womb. Suturing was done using standard surgical procedures, and post-surgical care was followed-up for ten-days. The heifer was successfully rehabilitated.
Urinary and bowel dysfunction is a condition that encompasses loss of bladder and bowel control which is characterized by partial or complete loss of defecation and urination. Urinary and bowel dysfunction is multifactorial and mainly... more
Urinary and bowel dysfunction is a condition that encompasses loss of bladder and bowel control which is characterized by partial or complete loss of defecation and urination. Urinary and bowel dysfunction is multifactorial and mainly caused by spinal cord injury at the lumbosacral level or more cranial. The present case report was aimed to show techniques and outcomes of colotomy to resolve constipation secondary to bowel dysfunction in a cat. A 1-year-old female cat weighing 1.3 kg was presented to Veterinary Teaching Hospital with a history of anorexia dullness, abdominal distension, and absence of defecation for the last 8-days. Clinical examination revealed stunted growth, poor body condition, very weak anal and pedal reflex, distended abdomen, dehydration, and lateral recumbency. Based on the history and clinical findings the case was diagnosed as constipation and exploratory laparotomy was indicated after sufficient stabilization. The caudal ventral midline was used to perform colotomy to remove accumulated feces. Post-operatively fluid therapy, antibiotics, laxative, and vitamins were administered accordingly. Upon 52-days follow-up; the patient was able to pass her feces completely, while urine retention and incontinence remains unresolved. Therefore, the owner was advised how to apply gentle external compression of the bladder to assist urination.
Leptospirosis is the most widespread and most prevalent zoonotic disease caused by Leptospira bacteria this is pathogenic Gramnegative bacteria, the disease leptospirosis a zoonotic disease of global importance. It caused by various... more
Leptospirosis is the most widespread and most prevalent zoonotic disease caused by Leptospira bacteria this is pathogenic Gramnegative bacteria, the disease leptospirosis a zoonotic disease of global importance. It caused by various serovars of leptospira interrogans that belong to the genus Leptospira. Besides leptospira interrogans is the most serovars like the disease occurs in nearly all mammalian species. The highest burden in low-income both found in urban and in rural environments. Rodents are known as the main reservoir animals, However, all animals are reservoir include human so leptospirosis is more common in the tropical regions rather than subtropics because of the longer survival of the organism in the environment and frequent exposure of animals and humans to contaminated environments. Direct transmission to humans by broken skin and mucous membranes exposed to the urine of infected animals. While the clinical signs of leptospirosis do not vary greatly with the species of animals include laboratory tests. Control and prevention is sanitary measures, vaccination, quarantine and rodent control are the most important control measures of the disease, require elimination of the carrier state, maintenance of environmental condition and immunization of the animal, tropical areas where warm humid conditions and alkaline or neutral soil helps leptospira survive better.
Aim This study was conducted by using the protocols of standard meat inspection (visual, palpation and incision) to assess the prevalence and associated risk factors and to estimate the economic loss in cattle slaughtered at Bedeno... more
Aim
This study was conducted by using the protocols of standard meat inspection (visual, palpation and incision) to assess the prevalence and associated risk factors and to estimate the economic loss in cattle slaughtered at Bedeno Municipal Abattoir, Eastern Hararghe, Ethiopia.
Method
A cross-section study design was conducted from February 2020 to November 2020, to determine the prevalence and its associated risk factors of bovine hydatidosis is taking animal origin, sex and age and body condition as risk factors.
Results
From A total of 355 cattle examined using the protocol of standard meat inspection, 99(27.8%) of them are found positive to hydatid cysts. The prevalence of cattle hydatidosis based on animal origin was highest in older cattle 89(29.47%) than young 10(18.89%). Regarding body condition, score to significance variation was observed, as the prevalence was 41.89% for poor cattle followed by medium 24.07% and good 24.37%. Of the total 99 infected, 41(11.55%) of animals were infected in their lungs with only hydatid cysts and 24(6.76%) were infected in their liver, but the rest animals had multiple organ infections, 34(9.58%). Considering the current result, the total annual economic loss from organ condemnation and carcass weight loss due to bovine hydatidosis at Bedeno Municipal Abattoir was estimated at 6,671,803.8 Ethiopian birr (ETB).
Conclusion
This study reveals that cattle hydatidosis was a high prevalence diseases in the study area and needs paucity of information on control and prevention. The cattle owners to be educated and awareness programmes to be conducted about the diseases.
Aim This study was conducted using the sedimentation method to assess the prevalence and associated risk factors of bovine fasciolosis around Furda Veterinary Clinic, East Hararghe, Ethiopia. Method A cross-sectional study design was... more
Aim
This study was conducted using the sedimentation method to assess the prevalence and associated risk factors of bovine fasciolosis around Furda Veterinary Clinic, East Hararghe, Ethiopia.
Method
A cross-sectional study design was conducted from October 2020 up to June 2021 to know the prevalence and its associated risk factors of bovine Fasciolosis, taking animal origin, sex, age and body condition as risk factors and standard deviation. The sedimentation technique was used for the recovery of fasciola eggs from fresh fecal.
Results
From a total of 384 animals selected randomly and coprologically examined, 117 of them contained fasciola eggs with a 30.5% total prevalence in the study area. The prevalence of bovine fasciolosis on the basis of animal origin was highest in Gorowodo 23 (28.7%), followed by Dada 24 (30.4%), Harawa 15 (20.5), Rasa Janata 25 (33.8%), and Tirtiro 30 (38.5%). Based on the sexes of animals, the prevalence rate of 74 (32.7%) in females and 43 (27.2%) in males were obtained. While animals of different ages were recorded at 43 (29.9%) and 74 (30.8%), respectively. Whereas 44 (33.6%), 45 (28.8%), and 28 (28.9%) were found to be in poor, moderate, and good physical condition, respectively. All of the risk factors investigated (animal origin, sex, age, and body conditions) were found to be non-significantly associated with the prevalence of bovine fasciolosis (p>0.05).
Conclusion
This study reveals that bovine fasciolosis was a highly prevalent disease in the study area and needs immediate control and prevention by the animal health office and veterinarians working together, treating cattle with anthelmintics before and after the rainy season, and increasing owner awareness about the disease.
Background Trypanosomosis is disease caused by unicellular parasites, trypanosome, found blood and other tissue of vertebrates; including livestock, wild life and people. It is a serious disease in domestic livestock causing a... more
Background
Trypanosomosis is disease caused by unicellular parasites, trypanosome, found blood and other tissue of vertebrates; including livestock, wild life and people. It is a serious disease in domestic livestock causing a significant negative impact on food production and economic growth in many parts of the world, particularly in sub-Saharan Africa. Its epidemiology and impact on livestock production are largely determined by the prevalence and distribution of the disease and its vectors in the affected area. Aim To assess the host related risk factors of bovine trypanosomosis and apparent density of tsetse flies in four peasant associations of the study area. In relation to the host risk factors, the prevalence of bovine trypanosomosis was highest in those animals with poor body condition.
Results
The overall 5.32% prevalence of bovine trypanosomosis was recorded from 432 blood sample collected from randomly selected animals using Buffy coat method. Trypanosoma congolense was the dominant species 14 (60.87%). However, it was not statistically significant between sex of animals (p>0.05). The mean packed cell volume (PCV) value of the infected animals was lower (20.65%±2.85) compared to non-infected animals (25.74%±4.80). There was statistically significant difference (p> 0.05). The mean packed cell volume (PCV) value of the infected animals was lower (20.65%±2.85) compared to non-infected animals (25.74%±4.80). There was statistically significant difference (p< 0.05) in the prevalence of trypanosomosis. Overall an apparent density of the flies was 2.42 f/t/d by using mono-pyramidal and biconical traps. It indicated that, G. morsitance submorsitance, G. pallidipes and G. tachinoides were tsetse flies species caught.
Conclusion
Finally, this work showed that trypanosomosis is an important disease affecting the health and productivity of cattle in the district. Hence, due attention should be given to this sector so as to improve livestock production and agricultural development in the area.
Dogs and cats are belonging to canine and feline family respectively. The liver is the largest gland in the body and is located in the cranial abdomen between 3rd and 4th ribs in dogs and cats. This review is mainly focused on: to... more
Dogs and cats are belonging to canine and feline family respectively. The liver is the largest gland in the body and is located in the cranial abdomen between 3rd and 4th ribs in dogs and cats. This review is mainly focused on: to understand the anatomy and physiology of liver, the liver diseases pathophysiology, to diagnose the liver diseases, managemental and ameliorative methods of liver diseases. The dual blood supply to the liver is hepatic artery and portal vein. The function of liver includes the regulation of digestion and metabolism, the synthesis of hormones and proteins, immune response and filtering of toxins from the blood stream. Any problem that affects the liver is liver disease. Inflammation of liver is hepatitis. Hepatitis caused by infectious, non-infectious, auto-immune and reactive. It can be acute and chronic. The most encountered liver diseases in dogs and cats are hepticlipidosis, cholangiohepatitis, portosystemicshunt, cholelithiasis, choledocholithiasis, cholecystitis, pneumobilia and hepatic neoplsia. The clinical symptoms of liver diseases include jaundice, hepatic encephalopathy, gastro intestinal disorders and nonspecific signs include polyuria/polydipisia. The liver disease diagnosed based on history, liver function tests, medical imaging. The latest imaging procedures are endoscopic retrograde cholagio pancreatography (ERCP) and computed tomography (CT). For confirmatory diagnosis liver biopsy and histopathological interpretation is required. Therefore, based on diagnosis appropriate treatment should be selected: bile stasis is treated urodeoxycholic acid (URDA), fluid therapy include sugar and salt solution for replacement fluid loss, gastrointestinal protectors include ranitidine, cimitidine and lactulose, albumin to treat hypoalbuminimia, antioxidant for scavenging free radicals include vitamin E, Selenium, S-adenosyl-L-methionine (SAMe), diuretic fursimid for treatment of ascites and supplementation low protein diets. This laparoscopic technique for removal of gallstones and endoscopic retrograde cholagiopancreatography (ERCP) to treat gall stones on bile ducts and to widen the slipped ducts and liver transplantation for cirrhotic dogs and cats. Early accurate diagnosis and managing any predisposing factors that affects the health of dogs and cats are important for controlling the liver diseases.
Aim This study was devised to be conducted to determine the prevalence and identify the major gastrointestinal nematode parasites based on fecal examination. Methods A cross-sectional study was carried out to determine the prevalence... more
Aim
This study was devised to be conducted to determine the prevalence and identify the major gastrointestinal nematode parasites based on fecal examination.
Methods
A cross-sectional study was carried out to determine the prevalence and associated factors with ovine gastrointestinal nematode infestation by fecal examination of 384 sheep from Wayu Tuka and Diga district, Eastern Oromia regional state.
Results
Out of the total 384 sampled sheep, 169 (44.0%) had been infected with gastrointestinal nematode parasite. Haemonchus species were the most frequently (20.8%) recovered nematode eggs followed by Trichostrongylus (13.0%) and Nematodirus (10.2%). There was no significant difference (p>0.05) in prevalence between age groups and sex. Sheep with poor body condition had a significantly higher prevalence of gastrointestinal nematode parasite (p>0.05) in prevalence between age groups and sex. Sheep with poor body condition had a significantly higher prevalence of gastrointestinal nematode parasite (p<0.05) than those sheep in moderate or good body condition. There was no significant association between the gastrointestinal nematode infection in animals of different ages and sex groups.
Conclusion
The study shows that the gastrointestinal nematode parasite was a major important health problem and impact on the production of sheep in the study area. Therefore, a detailed study should be conducted to identify the parasite at the species level and special consideration should be taken on the management of sheep in poor body condition to reduce the burden of gastrointestinal nematodes.
Cestode infestations in animals are the most important parasite of livestock and humans because most of these parasites are zoonotic causing cysticercosis and hydatidosis in man and it causes economic and production losses in livestock.... more
Cestode infestations in animals are the most important parasite of livestock and humans because most of these parasites are zoonotic causing cysticercosis and hydatidosis in man and it causes economic and production losses in livestock. Diagnosis of Taenia Spp by microscopic observation lack sensitivity and specificity and detection by enzyme-linked immunosorbent assay (ELISA) technique form cross-reaction. The molecular diagnostic can be best to detect in adult and larval stage in definitive and intermediate host based on the amplification of deoxyribonucleic acid (DNA) of target gene with the primer using a different technique of polymerase chain reaction (PCR) such as multiplex PCR. Conventional PCR, real-time PCR, nested PCR, and PCR-restriction fragment length polymorphism (RFLP) are highly sensitive for the diagnosis of cestode and metacestode. Those diagnoses are used for differentiation of Taenia species and differentiation of Taenia and Echinococcus species. As compared to other diagnostic techniques most molecular methods have higher sensitivity and specificity but due to the relatively higher cost, few are commercially available. Most of the molecular diagnostic tests developed to date are generally applicable for laboratory research purposes. The developments in the genomic and proteomic analysis should be used for further understanding of parasite-animal host interaction to find additional targets for diagnosis.
Background Culling is defined as the departure of cows from the herd because of sale, slaughter, salvage, or death. Culling is an important cost for dairy farms. At the same time, culling is a way to increase herd productivity and... more
Background
Culling is defined as the departure of cows from the herd because of sale, slaughter, salvage, or death. Culling is an important cost for dairy farms. At the same time, culling is a way to increase herd productivity and profitability, as keeping diseased and unproductive cows might result in lower herd milk production and deteriorated reproduction. In order to maximize profitability, the proportion of voluntary culling (selling for dairy purposes or culling due to low production) should be highest among the total culling rate. Previous studies indicate an ascending trend in the proportion of involuntary culling. This observational study used registry data of all cows from herds with ≥5 cow-years in 2016-2017.
Objective
This study was conducted to assess the reason for culling a dairy cow and its association with age at culling in and around Mekelle using a structured questioner format and direct observation. Visits were performed to each farm to collect data directly from owners or animal attendants and using direct observation.
Materials and Methods
The cross-sectional study was conducted in and around Mekelle city dairy farm from November, 2016 to April, 2017.
Results
The most common causes of culling were disease and economic reasons. The most common causes of voluntary culling were economic reasons (17.39), low milk yield (20.29%), and aging (8.70%). The common causes of involuntary culling were diseases (34.78%), injury (7.25%), and infertility (5.80%). In this study rates of culling increased with parity. The highest rate of culling was in animals between parity 2 and 5, increased from (14.49%) to (21.74%) and slightly decreased from parity 6 to 7, i.e. (13.04)% to (8.70)%, respectively. The highest rates of culling were between 3 and 5-years, (17.39)% and (23.19)%, respectively.
Conclusion
This study indicated animals were culled at premature age because of involuntary culling, which is economically not feasible, diseases were the most common causes of involuntary culling and voluntary culling was the most type of culling in dairy cattle farms and animals mostly culled for decreased production and economic reasons.
Pain is the distress feeling, most commonly encountered in diseases, which accompanies from an early age and it is one of the important aspects of determining the level of well-being of animals. Pain is more complex in animals and it can... more
Pain is the distress feeling, most commonly encountered in diseases, which accompanies from an early age and it is one of the important aspects of determining the level of well-being of animals. Pain is more complex in animals and it can rather difficult to determine the seriousness and impact of painful events. Hence, we have to identify the signs of pain in animals consistent with indirect markers which involve behavioral, physiological, and at last clinical responses. Moreover, specifically, the behavioral changes related to pain will be together with the overall signs also species-specific and hardly recognizable especially for an inexperienced observer seemingly unimportant which makes pain assessment even more complicated. The veterinarians have an ethical responsibility to assist in alleviate the animal pain. Although most veterinarians accept the actual fact that animals feel pain, still, post-operative pain relief isn't a routine practice in veterinary hospitals and clinics today the feeling and assessment of pain are subjective. The assessment of pain and also the documentation of the effectiveness of any interventions are fundamental principles of successful pain management. The most important aim is pain management while efforts are made to spot and treat the underlying disease and to reinforce healing and recovery. Adequate management of acute pain may additionally prevent the event of chronic pain.
Aim The study was conducted to determine the prevalence of hydatidosis, to identify the association between expected risk factors and occurrence of the disease, to investigate economic importance and to evaluate fertility and viability of... more
Aim
The study was conducted to determine the prevalence of hydatidosis, to identify the association between expected risk factors and occurrence of the disease, to investigate economic importance and to evaluate fertility and viability of the hydatid cyst in cattle slaughtered at the abattoir.
Materials and Methods
A cross-sectional study, which involves both ante-mortem and post-mortem examination, was conducted at Nekemte municipal abattoir from November 2015 to March 2016.
Results
A total of 355 local cattle breed was randomly sampled and examined for the presence of hydatid cysts in the organs of the animals using the standard meat inspection procedures, 66 (18.6%) animals were found harboring hydatid cysts. Statistical analysis showed that there was a significant difference (p<0.05) between the prevalence of bovine hydatidosis in all risk factors with exception of sex of the animals. Among 207 hydatid cysts recorded, 93 (44.92%) were from lungs, 65 (31.40%) from livers, 4 (1.93%) from heart, 3 (1.44%) from kidney, 1 (0.48%) from spleen and 41 (19.8%) were in two organs from lung and liver. Fifty-seven (57) of these 207 cysts were randomly selected and subjected to fertility and viability test which revealed 19 (33.33%) as fertile, 25 (43.86 %) sterile and 13 (22.81%) calcified. Viability test proved 7 (12.28%) of 19 fertile cysts as viable and 12 (21.05%) of 19 fertile cysts as non-viable. Viability of cysts in different organs revealed that 5 (16.1%) and 2 (10%) of cysts from lungs and liver, respectively. The estimated financial loss during the study period both due to condemnation of organs and an in direct carcass weight loss from hydatidosis was 2,190,143.52 ETB.
Conclusion
The results of this study revealed that bovine hydatidosis was an economically important disease of cattle which need serious attention for prevention and control actions in and around Nekemte. Hence, establishment of well-equipped standardized abattoirs, prohibition of backyard slaughter, creation of public awareness, deworming and control of stray dogs are of paramount importance.
Reproduction is one of the key pillars of production in many herds and individual animals. In female animals, reproduction can be affected by uterine diseases including metritis and endometritis. The objective of this review is to... more
Reproduction is one of the key pillars of production in many herds and individual animals. In female animals, reproduction can be affected by uterine diseases including metritis and endometritis. The objective of this review is to understand metritis and endometritis, diagnosis and treatment approaches, and the economic impact of uterine inflammations. Metritis is inflammation of the wall of the uterus, whereas endometritis is inflammation of the functional lining of the uterus, called the endometrium. Clinically, they are characterized by an enlarged uterus containing a watery red-brown fluid to viscous off-white purulent uterine discharge, which often has a fetid odor within 10 to 14-days after postpartum period in clinical metritis whereas clinical endometritis can also be observed its clinical signs after 21-days postpartum period. The risk factors for the occurrence of clinical metritis and endometritis include extrinsic factors include calving season and nutrition whereas intrinsic factors include retained fetal membrane (RFM), dystocia, metabolic disturbance, negative energy balance (NEB) and other non-specific diseases. These factors disturbing the normal physiology and weaken the immune system of dairy cattle. The factors favor the multiplication of pathogenic bacteria and cause inflammation of the uterus and results in metritis and endometritis. This result in increased conception interval increase the number of inseminations, loss of milk and meat production, marked drop in fertility and increase the cost of treatment. Clinical metritis and endometritis can be diagnosed by clinical symptoms and cytological examinations. Clinical metritis and endometritis can be treated by anti-inflammatory drugs flunixin meglumine, antibiotics (pens-trip and long-acting oxytetracycline), intrauterine infusion by using saline or iodine tincture solutions. Clinical metritis and endometritis are the most frequent uterine disorders in dairy cows causing decreased fertility leading to high economic losses. Therefore, management of these production diseases can be achieved by early diagnosis and treatment, supplementation of balanced feed containing minerals, vitamins, appropriate proteins and carbohydrates, good quality management during and after calving of the female animals.
Nanoparticles are small and have a large surface-to-volume ratio, which gives them phenomenal, unique features. It is an emerging, multidisciplinary field that frequently employs new techniques and tools from diverse disciplines,... more
Nanoparticles are small and have a large surface-to-volume ratio, which gives them phenomenal, unique features. It is an emerging, multidisciplinary field that frequently employs new techniques and tools from diverse disciplines, including biology, engineering, chemistry, and medicine. Nanotechnology entities help to improve the solubility, absorbability, bioavailability, and half-life of conventional natural products. Nano-applications are used in poultry and animal production systems using available tools and techniques without affecting animal health and welfare. Nanotechnology is a smart technology in the field of biomedical engineering used for the diagnosis and treatment of different poultry diseases. This technology provides better solutions for various applications and poultry production that can help in reducing costs and improving the final product quality. Even though nanotechnology is one of the main novelties which have already been applied in poultry and other different areas, it is still in the initial stages of its development and it hampers the environment, animal, and people’s health. Therefore, wide hazard assessments should be conducted to ensure the safety of the nanoproducts before making them immediate implementation for poultry/animal/ or human use.
A three-months-old Holstein Friesian crossbred calf weighing 56 kg was presented to Veterinary Teaching Hospital, Mekelle University, Ethiopia with a history of swelling at the umbilical region from birth since last few months. Clinical... more
A three-months-old Holstein Friesian crossbred calf weighing 56 kg was presented to Veterinary Teaching Hospital, Mekelle University, Ethiopia with a history of swelling at the umbilical region from birth since last few months. Clinical examination revealed
umbilical hernia with a hernial ring of 3 fingers breadth. The case was handled surgically by means of herniorrhaphy using overlapping mattress suture techniques and synthetic absorbable polyglycolic acid. The calf has unevenly recovered on the 20th-day
post-operation. The present case report discusses the successful management of umbilical hernia in the calf.
Background Equines play a crucial role in the Ethiopian economy; however, their welfare is extremely poor. Wounds, eye problems, lameness, hoof problems, parasites, and poor body condition were the most dominant welfare issues identified... more
Background
Equines play a crucial role in the Ethiopian economy; however, their welfare is extremely poor. Wounds, eye problems, lameness, hoof problems, parasites, and poor body condition were the most dominant welfare issues identified from different part of the country.
Aim
The objective of this study was to assess the socio-economic importance and constraints of equines in and around Debre Berhan town, North Shewa Zone of the Amhara region, Central Ethiopia.
Material and Methods
A cross-sectional questionnaire-based survey was carried from November 2016 to April 2017 and a total of 150 equine owners from the three districts namely Debre Berhan (97), Basonaworana (41), and Chacha districts (12) were interviewed during the study period.
Results
Accordingly, 66 (44%) and 46 (30.67%) of respondents use their equines for packing and carting. Besides, equines were also used for other social activities such as a funeral (2%), marriage (5.33%), religious ceremonies (23.33%), and mixed uses (69.34%). Diseases (46.67%), feed shortage (26%), water shortage (18%), and market unavailability of what (9.33%) were among the major constraints identified in the area. Moreover, gastrointestinal parasites (30%), back sore (16.67%), lameness (21.33), colic (12%), and other diseases (20%) were the major health constraints of working equines in the study area.
Conclusion
The welfare and management of working equines in the study sites need more close attention and should be improved. Awareness creation through training in the welfare problems of equines is advantageous to fully exploit these animals.
Tail docking is usually performed on those dogs whose tail or associated structures have been injured. A two-year-old male dog was presented Veterinary Teaching Hospital (VTH) with complain of injury on the tail due to bite by another... more
Tail docking is usually performed on those dogs whose tail or associated structures have been injured. A two-year-old male dog was presented Veterinary Teaching Hospital (VTH) with complain of injury on the tail due to bite by another dog. The injured site of the tail was contaminated with the debris on presentation and the dog was in severe pain. Amputation of the tail at the level above the injury site of the tail was taken. Care for surgery, oxytetracycline wound spray was applied on the incision site of the tail; Pen Strep and Meloxicam were given for three-days. The suture material was removed 10th-day post-surgery and the tail stump healed uneventfully. Hence, tail amputation is the best option of treatment for severing traumatic tail and successful management of the injured tail was presented in the present case report.
A 2-year-old American Pitbull Terrier bitch weighing 25 kg was presented to Teaching Veterinary Clinical complex at College of Veterinary and Animal Science, Udgir District, Latur, Maharashtra, India on 24/02/2020 with a history of... more
A 2-year-old American Pitbull Terrier bitch weighing 25 kg was presented to Teaching Veterinary Clinical complex at College of Veterinary and Animal Science, Udgir District, Latur, Maharashtra, India on 24/02/2020 with a history of convulsions, restlessness, severe howling, muscle tremors along with inappetance for 1-day and muscle weakness. On obtaining the history from owner it was found out that the owner had accidentally fed the bitch Hitex (Ivermectin) bolus of 80 mg.
A Holstein Friesian (HF) crossbred dairy cow of 5-years-old and 38.5-weeks pregnant was presented to the Veterinary Hospital of College of Veterinary Science, Mekelle University with a history of repeated prolapse since last 10-days. Upon... more
A Holstein Friesian (HF) crossbred dairy cow of 5-years-old and 38.5-weeks pregnant was presented to the Veterinary Hospital of College of Veterinary Science, Mekelle University with a history of repeated prolapse since last 10-days. Upon clinical examination, the case was confirmed as prepartum cervico-vaginal prolapse due to excess labor and straining to deliver the fetus. After caesarean section, the cow was restrained on sternal recumbency and the prolapsed mass was made aseptic by washing with a 2% potassium permanganate solution and was repositioned into the pelvic cavity. The cow was kept on fluid therapy (stat), antiinflammatory and antibiotic for three successive days. A modified Buhner’s technique, using infusion set tube as suture was to keep the prolapsed tissue in position to prevent the recurrence and the cow had an uneventful recovery. A successful management of recurrent prepartum cervico-vaginal prolapse using modified Buhner’s suture after caesarean section in a crossbred Holstein Friesian dairy cow is discussed.
Aim The objective of this study was to assess the major welfare problems in working donkeys in Hosaena district, Hadiya Zone, Southern Ethiopia. Methods and Materials A total of 318 male and 76 female working donkeys were randomly... more
Aim
The objective of this study was to assess the major welfare problems in working donkeys in Hosaena district, Hadiya Zone, Southern Ethiopia.
Methods and Materials
A total of 318 male and 76 female working donkeys were randomly selected using a cross-sectional study from November 2015 to April 2016.
Results
According to the current investigation, 70% of donkeys were used for draught and 30% were used for pack type of work. All females (100%) were used for packing purpose, whereas 86.8% of male donkeys were used for draught purpose. Overall, 41.6% of donkeys had a poor body condition in all age groups, whereas most donkeys (44.3%) under the age group less than 5-years were having poor conditions. Regarding wound on the skin, a higher proportion (82.2%) of back lesions were observed in pack donkeys whereas lip lesions were observed in 87.7% of draught donkeys. Besides, the welfare problem was observed in 28.9% of working donkeys due to overloading. A significant association (p<0.01) was found between the duration of working hours of donkey and poor body condition. Out of the interviewed, the majority of animal owners (76.4%) had one donkey.
Conclusion
The assessment showed that working donkeys in the present study area were experiencing multiple welfare problems. Accordingly, awareness creation through mass education, training and extension service should be promoted in the study area in order to ensure better donkey welfare and productivity.
Prolonged pregnancy is defined as a pregnancy that lasts for more than 294-days compared with term gestation which is between 260 and 293-days. This case report was prepared with the aim of describing and documenting the surgical... more
Prolonged pregnancy is defined as a pregnancy that lasts for more than 294-days compared with term gestation which is between 260 and 293-days. This case report was prepared with the aim of describing and documenting the surgical procedures, techniques of cesarean section and its outcome on eight-years-old crossbred cow that was referred to the veterinary hospital, Mekelle University from a field; Edaga Hamus Veterinary Clinic. According to the owner’s history, the cow was presented to the clinic with an extended gestational period over 345-days than anticipated according to their own record. Thus, based on the history and clinical findings, the case was diagnosed as prolonged pregnancy. After aseptic preparation of the surgical site, stabilizing the animal and locally desensitizing the incision area; a vertical skin incision with a distance of approximately (~40 cm wide) on the left flank region approximately (~10 cm) below the lumbar transverse process. After the incision of the abdominal muscles and uterus, the non-viable and abnormally oversized calf was taken out with the help of an assistant. Moreover, the uterus was filled with excess and abnormal dark brown colored blood and was properly drained, washed with sterile isotonic saline solution. The uterus was closed using a double layer of Utrecht suturing pattern whereas the peritoneum and abdominal muscles were separately closed with a continuous lockstitch pattern and simple interrupted pattern using 2-0 size sterile absorbable polyglycolic acid, respectively. After that, the skin was closed using silk 2-0 size in a horizontal interrupted mattress. Lastly, with proper antibiotic follow-up, dressing and cleaning of the surgical site, the cow was successfully recovered after two months.
Organic livestock farming is a useful strategy to overcome the challenges of the agricultural sector like sustainability, food security, and food safety while matching with a high status of animal welfare, care for the environment,... more
Organic livestock farming is a useful strategy to overcome the challenges of the agricultural sector like sustainability, food security, and food safety while matching with a high status of animal welfare, care for the environment, restricted use of medical drugs, growth promoters, synthetic fertilizer and the production of healthy food products without drug residues. Furthermore, organic livestock farming could also be an interesting strategy for the eternal rural development issue and solution for the farms’ decreasing profitability. The regulation of organic animal production is comprehensive and affected by different aspects such as feeding, housing, breeding, health care, and medical treatment. In contrast to conventional production systems, animals under organic livestock production are allowed a larger housing area, have obligatory straw bedding, fed organic feed, and roughage. The animal feed is a strong determinant of the fatty acid composition of the milk, egg or meat. In relation to animal health and welfare, the application of breed selection, good management, nutrition, and husbandry plays a greater role in disease prevention and good animal welfare. Now-a-days, antimicrobial resistance is a global public health issue and a threat to the modern health care system which is recognized by many international health organizations. Accordingly, over the last few years, the demand for organic products and availability in the market has been increased and made significant development in the economy. Several people have started to consider organic food instead of conventional food because it is healthier and less prone to the impact of chemical residues. Consequently, awareness creation and encouraging the importance of organic products for customers is important for the development of organic production.
Brucella is intracellular bacteria that cause brucellosis, a chronic zoonotic disease. The genus of Brucella are subdivided into six species categorized by antigenic variation and primary preferred host and these include B. abortus, B.... more
Brucella is intracellular bacteria that cause brucellosis, a chronic zoonotic disease. The genus of Brucella are subdivided into six species categorized by antigenic variation and primary preferred host and these include B. abortus, B. melitensis, B. suis, B. ovis, B. canis and B. neotomae. The epidemiologies of the disease in livestock and humans, as well as appropriate preventive measures, are not well understood in developing countries. Materials excreted from the female genital tract acting as the main supply of organisms for transmission to other animals and human. Millions of individuals are at risk worldwide, especially in countries where infection in animals has not been brought under control, procedures for the heat treatment of milk, such as pasteurization are not routinely applied, and standards of hygiene in animal husbandry are low. A precise diagnosis of brucellosis is important for the control of the disease in animals and consequently in human. Molecular and serological techniques are important tools for diagnosis and epidemiologic studies, providing relevant information for identification of species and biotyping. The economic and public health impact of bucellosis remains particular concern and neglected in developing countries. The disease mainly hampers the productivity of small ruminant’s resulting infertility and increase the average inter-calving periods. One of the major gaps in our knowledge at present is the relative contribution of brucellosis on small ruminant and humans. In Ethiopia, no strategy is in place to control brucellosis. The most important approach to the control and prevent human of brucellosis in human and animal is the practice of one health approach. So knowing the status of small ruminant brucellosis in our country is therefore extremely important. Therefore, the aims of this review are; a) To highlight (snapshot) of brucellosis in the small ruminant; b) To show the seroprevalence status of small ruminant brucellosis in Ethiopia perspective; c)To highlight possible risk factor and its economic importance.
Probiotics are live microbial feed supplements, which have an effect on the host animal by means of enhancing its intestinal microbial balance. A probiotic is a culture of a single bacterial strain, or a mixture of different strains, with... more
Probiotics are live microbial feed supplements, which have an effect on the host animal by means of enhancing its intestinal microbial balance. A probiotic is a culture of a single bacterial strain, or a mixture of different strains, with the aim of eliminating the colonization of pathogens in the gastrointestinal tract of poultry. The main sources of probiotics in poultry are strains of microorganisms such as Lactobacillus, Enterococcus and Bacillus and fermented dairy products like yoghurt, cultured buttermilk and cheese. A good probiotic is characterized by its ability to exert a beneficial effect on a host, resistance to low pH and bile salts, adhere and colonizing of the intestinal epithelium, non-pathogenic to host and produces antimicrobial substances towards pathogens. It also boosts immune responses, improves the growth performance and productivity of poultry and increases the quality of meat and egg. Thus, probiotics are considered to fill the gap in the poultry industry due to diseases and antimicrobial resistance of pathogenic bacteria as well as environmental conditions that cause serious problems and economic losses in many countries. With current consumer preferences tending toward purchasing products from livestock grown without antibiotics and feed additive, the ingredients in this review paper presented the beneficial applications probiotic may have in poultry production.
Foodborne diseases are a public health threat which causes a large economic impact across the worldwide. Escherichia coli (E. coli), Listeria monocytogenes (L. monocytogenes), Salmonella species (S. species), Staphylococcus aureus (S.... more
Foodborne diseases are a public health threat which causes a large economic impact across the worldwide. Escherichia coli (E. coli), Listeria monocytogenes (L. monocytogenes), Salmonella species (S. species), Staphylococcus aureus (S. aureus) and many more other organisms are the leading causes of foodborne illness and death in the world. Increment of antibiotic resistance exhibited by the actions of microbial infectious agents has led to screening of several medicinal plants for their potential antimicrobial activity. Therefore, the aim of this paper is to review on the medicinal value of Croton macrostachyus (C. macrostachyus) and Solanum incanum (S. incanum) against causative agents of foodborne disease. Antimicrobial compounds of medicinal plants differ from antibiotics as they have fewer side effects, better patient tolerance, relatively less expensive, acceptance due to a long history of use and being renewable in nature. There are so many medicinal plants used to treat foodborne diseases which associated with gastroenteritis in humans and animals, among plants S. incanum and C. macrostachyus are the common for treatment of foodborne diseases associated with diarrhea. S. incanum has different bioactive substances which have medicinal importance against skin diseases, abdominal pains, fever, stomachaches and indigestion, treatment of dandruff, wounds, sore throat, angina, ear inflammation, liver disorders, wart and ringworm and treatment of cow driosis, dermatophilosis, foot rot, pasteurellosis, black leg, fasciolosis and snake bite. Bioactive compounds which present in S. incanum are including alkaloids, steroids, saponins, tannins, glycosides, flavonoid and terpenoids. C. macrostachyus is medicinal plant which have bioactive compounds including terpenoids, alkaloids, flavonoids, lignoids, proanthcyanidins, sesquiterpenoids and lupeol, saponnins, resins, crotepeoxide. C. macrostachyus have medicinal value in treatment of malaria, rabies, gonorrhea, wound, diarrhea, hepatitis, jaundice, abdominal pain, cancer, toothache, pneumonia, typhoidand gastrointestinal disorder. Which is also used as abortifacient and uterotonic to expel retained placenta. Therefore, S. incanum and C. macrostachyus have different medicinal value against foodborne disease and drug-resistance infectious agents. However, an advanced study have not been conducted on the extract of pure bioactive compounds and toxicity analysis. Therefore, further studies should have to be conducted to extract pure compounds from these medicinal plants for pharmaceutics industry.
Non-surgical sterilization technique in animals is an ancient practice and dates back to 7000 BC. Surgical castration in animals has been applied for centuries to control the animal population, advance genetic selection, improve calmness... more
Non-surgical sterilization technique in animals is an ancient practice and dates back to 7000 BC. Surgical castration in animals has been applied for centuries to control the animal population, advance genetic selection, improve calmness of aggressive animals and mainly to ensure and selectively provide high-quality meat production for human consumption. An ideal method of castration should cause permanent block to spermatogenesis and inhibit androgenetic enzymes with low-cost of treatment and doesn’t affect the welfare of the animal. Even if operative methods are the main methods of castration, hormonal castration and chemical castration are also an alternative and equivalent method of sterilization. Over the past years, non-surgical sterilization has found application in male dogs, cats, monkeys, goats, bulls, hamsters and rabbits. Calcium chloride, lactic acid, sodium chloride, chlorhexidine, formalin, zinc tannate, zinc gluconate, glycerol, glucose, ethanol and silver nitrate are commonly used in chemical castration. After intratesticular application, degeneration of seminiferous tubules and Leydig cells, decrease in testosterone and sperm production, testicular atrophy is observed. In this review, the approaches of chemical castration were mentioned in different male animals.
Dehorning or disbudding is the process of removing or stopping the growth of the horns and horn producing tissues after the horns have formed from the bud by different methods which can match to the size of the horn and age. The present... more
Dehorning or disbudding is the process of removing or stopping the growth of the horns and horn producing tissues after the horns have formed from the bud by different methods which can match to the size of the horn and age. The present case report is aimed to describe and document the surgical procedures, techniques of unilateral horn amputation and its outcome on six years old local breed cow that was referred to the veterinary teaching hospital (VTH), Addis Ababa University from nearby; Hiddi Veterinary Clinic. History stipulated as the cow was treated several times with antibiotics but didn’t respond as the condition was getting worse. Based on the history and clinical findings, the case was diagnosed as suppurative frontal sinusitis. After aseptic preparation of the surgical site, stabilizing the animal and locally desensitizing the incision area; an elliptical skin incision with a distance of approximately (~1 cm wide) around the base of a right horn was performed for successful removal of the corium. Then, skin edge was opposed to assist the skin contraction by using the silk 2-0 size in cross mattress suture pattern. Then the area was properly bandaged with elastic bandages and properly secured to the normal horn and admitted home. Finally, with regular dressing, bandaging and lavaging of the dehorned site, the cow was successfully recovered after two months.
Aim The objectives of this study were to assess the prevalence of major gastrointestinal tract (GIT) parasite of cattle’s and associated major risk factor at Jimma municipal abattoir. Method A cross-sectional study with a simple random... more
Aim
The objectives of this study were to assess the prevalence of major gastrointestinal tract (GIT) parasite of cattle’s and associated major risk factor at Jimma municipal abattoir.
Method
A cross-sectional study with a simple random sampling method was conducted from November, 2018 to April, 2019.
Result
Based on the carpological examination, from 400 animals that were presented for slaughter at Jimma municipal abattoir, 46.8% (187) of animals have at least one GIT parasite. The study detected five genera of GIT parasite which were Strongyle-type, Trichuris spp., Monezia spp., Paramphistomum spp. and Eimeria spp. with prevalence of 28.9% (54), 4.8% (9), 3.2% (6), 38.5% (72) and 13.4% (25), respectively while mixed parasites has 11.2% (21) of prevalence. The diversity of those mixed parasite were Strongyle type with Paramphistomum spp., Paramphistomum spp. with Eimeria spp., Eimeria spp. with Trichuris spp., Strongyle spp. with Monezia spp., Paramphistomum spp with Monezia spp,, Strongyle type with Emeria spp. and Paramphistomum spp. with Trichuris spp. with the prevalence of 42.9%, 14.3%, 14.3%, 9.5%, 14.3%, 23.8% and 10.0% respectively. The prevalence of gastrointestinal parasite based on origin of the study animals was recorded and showed statistically significant difference with p-value of 0.001 (p≤0.05) and the prevalence was highest in Seka Chekorsa and followed by Kersa, Mena, Oma Nada and Dedo in decreasing order. The risk factor related to the age showed statistically significant difference (p<0.05) in which GIT parasite has highest prevalence in young and least in old and moderate in adult cattle. This study showed that infection prevalence was highest in animal with poor body condition followed by medium and good body condition scores and difference was statistically significant (p<0.05).
Conclusion
The finding showed that good management and strategic anthelmintic treatment need to be applied in the area to reduce the prevalence of the GIT parasites of cattle and their risk factor to lessen economic loss caused by the parasite.
There is a diversified microbial ecosystem in the rumen for efficient utilization of diet by providing essential nutrient to their host. But there are different factors affecting rumen microbial protein synthesis which are physical... more
There is a diversified microbial ecosystem in the rumen for efficient utilization of diet by providing essential nutrient to their host. But there are different factors affecting rumen microbial protein synthesis which are physical factors, chemical factors, dietary factors, biological factors and endogenous factors. Among the details of factors, dietary factors and ruminal pH are the dominant factors influencing rumen microbial protein production. The effects of some dietary factors, on the amount and efficiency of microbial protein synthesis, are discussed in this review. Specifically, these factors include forage quality diets, level of feed and types of feed. It seemed that diets containing a mixture of forages and concentrates increase the efficiency of microbial protein synthesis because of an improved rumen environment for the growth of more diverse bacterial species. This review describes physical and chemical factors which include: pH and buffer system, oxygen concentration, rumen outflow rate and synchronized release of nitrogen and energy from the diet, a nitrogen compound, energy spilling, vitamins and minerals and antimicrobials chemicals, respectively. Age, species, physiological status, sex, and stress are among endogenous factors that mostly affect microbial protein synthesis of a ruminant. Bacteriophages, protozoa predation and bacterial lysis are biological factors affecting the efficiency of microbial protein synthesis. All these factors have a direct effect on the synthesis of microbial protein in the rumen. Therefore, the cumulative effects of the above factors are resulted in the depopulation of rumen microflora and finally reduction of animal product. So, improvement in quantitative aspect of microbial protein synthesis solves many problems from simple to complex so that, the quantitative aspect of rumen microbial biomass are invaluable for health and productivity of ruminants than qualitative aspect hence, maintain health rumen ecosystem means having healthy ruminant.
The records of 1085 crossbred cattle daughters of 57 sires maintained during 1974-2004 at Instructional Dairy Farm of Govind Ballabh Pant University of Agriculture & Technology, Pantnagar, Uttarakhand, India were used to evaluate sires... more
The records of 1085 crossbred cattle daughters of 57 sires maintained during 1974-2004 at Instructional Dairy Farm of Govind Ballabh Pant University of Agriculture & Technology, Pantnagar, Uttarakhand, India were used to evaluate sires for first lactation performance traits. The data were analyzed to estimate the breeding values of sires using least squares methods (LSM), best linear unbiased prediction (BLUP) and derivative free restricted maximum likelihood (DFREML) method. The average breeding values of sires estimated by all 3 methods were similar and estimated for age at first calving (AFC), first lactation period (FLP), first dry period (FDP) and first calving interval (FCI) as 1359.95 days, 356.56 days, 177.58 days and 534.15 days respectively. The estimated breeding values (EBV’S) showed large genetic variation between sires for first lactation. The EBV’S of sires had very high and significant product moment correlations and rank correlations among all first lactation traits. These results revealed that estimated breeding values of sires estimated for first lactation traits by LSM method showed small genetic variation in comparison to BLUP and DFREML methods. The LSM method was found to be more efficient, accurate, and stable with lowest genetic variation.
Almost all the phages reported were long-tail or short-tail, belong to the Myoviridae or Podoviridae phage families, little information was available on tailless bacteriophages and their biological significance in therapy. Tailless... more
Almost all the phages reported were long-tail or short-tail, belong to the Myoviridae or Podoviridae phage families, little information was available on tailless bacteriophages and their biological significance in therapy. Tailless bacteriophage φHN161 in this study was isolated from sewage water samples. This representative phage displayed a strong ability in eliminating pathogenic Escherichia coli O161. Mice challenged with E. coli O161 were treated with different doses of phage φHN161 for comparing sterilization rate and immunological reaction with those untreated mice in control group. Phage of higher dose was much more effective than of lower dose in eliminating host cells both in vitro and in vivo, while a lower dose of phage treatment could drastically reduce inflammation caused by E. coli O161 infection. Altogether, these observations expanded the diversity of phages that could be utilized as antimicrobial agents substitute in animals; these findings also reminded people to pay attention to the dosage of bacteriophages used in therapy.
Bovine mastitis, which is an inflammation of the mammary gland frequently resulting from bacterial infection, causes the majority of economic losses to the dairy producers. The prevalence of mastitis in dairy cattle is relatively high.... more
Bovine mastitis, which is an inflammation of the mammary gland frequently resulting from bacterial infection, causes the majority of economic losses to the dairy producers. The prevalence of mastitis in dairy cattle is relatively high. Bovine mastitis can be classified based on clinical futures as clinical orsubclinical. Both forms produce significant economic losses due to rejected milk, degraded milk quality, early culling of cows, drug costs, veterinary expenses and increased labour costs for the farmer. Moreover, subclinical mastitis is the main form of mastitis in modern dairy herds, exceeding 20 to 50% of cows in given herds. The cost of subclinical mastitis is very difficult to quantify, but most of the researchers agree that it can be up to 40 times more common than clinical mastitis. In India, the estimated loss due to mastitis is around Rs. 16,702 million per annum. Subclinical mastitis was found more important in India than clinical mastitis especially in cows (10-50%) and buffaloes (5-20%). The predominant causal organisms of mastitis are cell-walled bacteria, although mycoplasma, yeast and algae have also been reported to cause mastitis. Various predisposing factors like physiological, genetic, pathological or environmental may contribute to the emergence of mastitis in dairy cattle. Presently antibiotics are used for the treatment of mastitis. However, therapeutic success rate is poor due to indiscriminate use of antibiotics leading to development of multiple drug resistant pathogens. Besides this, major problems in bovine mastitis with intracellular pathogens like S.aureus because of poor cure rate. These facts highlight the need for completely newer strategies for treatment of mastitis.
Cryopreservation of semen plays an important role in the preservation of genetic resources and its efficiency has been affected by compromised post-thaw sperm quality.1 Considerable evidence suggests that genetic differences have... more
Cryopreservation of semen plays an important role in the preservation of genetic resources and its efficiency has been affected by compromised post-thaw sperm quality.1 Considerable evidence suggests that genetic differences have contributed to the wide variations in post-thaw semen quality, and have encouraged the selection of animals whose semen can withstand the cryopreservation procedure.2 In the case of boar, variations in semen freezability have been shown to be related to molecular genetic markers.2 However, a thorough analysis of the specific genetic markers facilitating freezability will be the most efficient approach to improve the technology of semen cryopreservation. Specifically in the boar, such novel approach is based on transcriptome analysis of ribonucleic acid-sequencing or RNA-sequencing (RNAseq) data from spermatozoa from individuals with poor and good semen freezability. Moreover, transcriptome analysis of spermatozoa has identify potential messenger RNA (mRNA) profiles that could serve as marker for bull semen freezability has been reported.3 So far, RNA-seq data have been widely used in the analysis of different cellular tissues, but its application in transcriptome study on semen freezability is limited. Therefore, it is hypothesized that transcriptome study on poor and good freezability ejaculates, in conjunction with bioinformatics studies, will provide a more comprehensive approach for the identification of novel sperm-specific gene transcripts facilitating sperm cryo-tolerance.
Background Urocystoliths are difficult to palpate and physical examination findings, complete blood cell count (CBC) and serum biochemical analysis are usually normal and the clinical signs are not definitive. Thus diagnostic imaging is a... more
Background
Urocystoliths are difficult to palpate and physical examination findings, complete blood cell count (CBC) and serum biochemical analysis are usually normal and the clinical signs are not definitive. Thus diagnostic imaging is a crucial tool required to confirm the diagnosis of urolithiasis in dogs presented with non-specific clinical signs of urogenital affection.
Aim
The aim of this study was to compare the capability of radiography and ultrasonography in detecting uroliths and concurrent urinary system abnormalities and to evaluate clinical, haematological and urinalysis findings of dogs affected with urolithiasis during the presentation.
Methods
Findings of signalment, history, physical and laboratory examination of blood and urine were performed and recorded. All dogs presented with complete or partial urinary obstruction, haematuria and renal failure were subjected to both radiographic and ultrasonographic evaluation. Uroliths were retrieved by a cystotomy, urethrotomy, and at necropsy from kidney failure cases confirming urolithiasis.
Results
The result revealed occult clinical haematuria in 56.5%, microscopic haematuria in 78.3% and dysuria/anuria in 34.8% of the affected dogs. Crystalluria is detected in seven (30.4%) of urolithiasis affected dogs. The total leukocyte count was significantly elevated (p≤0.05) in partially and completely obstructed dogs. Radiography diagnosed 19 of 23 urolithiasis cases in the urinary bladder (UB), 2 of 2 in the kidney and 12 of 13 in the urethra while ultrasonography diagnosed 17 of 23 urolithiasis cases in the UB and one in the urethra. From a total of 15 dogs presented with either neoplastic growth and/or cystitis concurrent with urolithiasis, ultrasound detected six while pneumocystogrpahy detected only one.
Conclusion
The study showed haematuria as the leading clinical sign of urolithiasis. Detection of urolithiasis and concurrent cystitis and/or urinary bladder growth increases when ultrasonography and radiography were employed together.
Infectious bursal disease (IBD) is an infectious viral disease of poultry. It is caused by infectious bursal disease virus (IBDV) that is a member of the genus Avibirnavirus of the family Birnaviridae. The virion is non-enveloped and... more
Infectious bursal disease (IBD) is an infectious viral disease of poultry. It is caused by infectious bursal disease virus (IBDV) that is a member of the genus Avibirnavirus of the family Birnaviridae. The virion is non-enveloped and consists of a bi-segmented RNA molecule. The disease occurs in a clinical and subclinical form depending on age at infection. Only young chickens are clinically affected. Severe acute disease of 3-6 week old birds is associated with high mortality but a less acute or subclinical disease is common in 0-3-week-old birds. This can cause secondary problems due to the effect of the virus on the bursa of Fabricius. There are two serotypes of IBDV; These are serotype 1 which is pathogenic to chickens and commonly leads to the development of the clinical form of the disease and serotype 2 is avirulent to chickens. Clinical IBD can be diagnosed by the combinations of a characteristic sign and post-mortem lesions. Gross lesions are characterized by marked haemorrhages in the pectoral and thigh muscles. At post-mortem examination bursa of Fabricius, thymus, spleen and kidneys are initially enlarged, however, bursa of Fabricius and thymus are later become atrophic. Histologic lesions showed marked edema, infiltration of heterophiles, hyperaemia and lymphoid depletion and hyper plastic corticomedullary layer in the bursa of Fabricius. Serological diagnosis of IBD by agar gel immunodiffusion (AGID), enzyme linked immunosorbent assay (ELISA), virus neutralization test (VNT) and agar gel precipitin test (AGPT) are also possible. Subclinical IBD can be confirmed in the laboratory by detecting viral antigens in tissues. In the absence of such tests, histological examination of the bursa may be helpful.
Background and Aim The study was conducted at Dida Tuyura Cattle Breeding and Improvement Ranch in Borana zone, southern Ethiopia, with the aims of determining the mortality rate of Ethiopian Boran calves and assessment of the... more
Background and Aim
The study was conducted at Dida Tuyura Cattle Breeding and Improvement Ranch in Borana zone, southern Ethiopia, with the aims of determining the mortality rate of Ethiopian Boran calves and assessment of the reproductive performance of heifers and cows.
Materials and Methods
The study was based on the retrospective record of calves, heifers, and cows. The data was taken from 1994-2010 on the calf survival rate and 1994-2005 on reproductive performance of the breed in the ranch were used for this study. The retrospective data collected over the years were used to identify factors associated with calf survival or death.
Results
The mortality rate of calves before weaning and after weaning was 13.3% and 11.2%, respectively. Age, sex and birth weight of calf had a significant effect on survival rate. The overall mean values age at first service, age at first calving, calving interval, days open and gestation length were 42.52, 51.67, 20.67, 11.30 and 9.30 months, respectively. Parity had a significant effect on both the calving interval and days open. Gestation length was not significantly affected by parity, sex, and birth weight of calf.
Conclusion
From the present study, it can be concluded that the obtained calf survival rate and reproductive performance are not sufficient to achieve the established objective of the ranch.
Porcine epidemic diarrhea virus (PEDV) is an economically relevant viral agent associated with diseases in pigs. In a farm which has experienced PEDV outbreak, the infected pigs show severe alimentary system disorders characterized by... more
Porcine epidemic diarrhea virus (PEDV) is an economically relevant viral agent associated with diseases in pigs. In a farm which has experienced PEDV outbreak, the infected pigs show severe alimentary system disorders characterized by symptoms such as vomiting, watery diarrhea, dehydration and growth retardation, and the young piglets usually have a higher mortality rate.1,2 As a member of the Coronaviridae family and the Nidovirales order, PEDV is an enveloped, positive-sense, single-stranded RNA virus. The PEDV genome comprises of a 5’- and 3’- terminal untranslated region (UTR) flanking a large replicase gene and a couple of structural protein genes, approximately encompassing 28 kb size in length.3
Background: There are various emerging scientific evidences based on research trials and therapeutic practices that 'Synbiotics' (probiotics and prebiotics) play an important role in animal health and nutrition. There has been a... more
Background: There are various emerging scientific evidences based on research trials and therapeutic practices that 'Synbiotics' (probiotics and prebiotics) play an important role in animal health and nutrition. There has been a significant increase in the characterization and verification of potential health benefits associated with the use of probiotics and prebiotics.
Objective: The primary clinical effects for the application of probiotics have been reported as the ability to modulate the balance and activities of the gastrointestinal (GI) microbiota, treatment of infectious diseases including viral, bacterial and antibiotic associated diarrhea, cellular immune-modulation, lowering of serum cholesterol, improvement in lactose digestion, allevia-tion of allergy related disorders, reducing the risk of colon cancer and imparting the coloniza-tion resistance effect on intestinal microbiota.
Results: The probiotic therapy also known as the microbial interference therapy (MIT) has in particular drawn the interest of animal clinicians, in the treatment of enteric infections of neonatal farm and food animals eliminating the entero-pathogens selectively while building up the normal intestinal flora to flourish. This cannot be achieved by the use of gut-active antibiotics , which lack the ability to discriminate between its friends and foes. The probiotic therapy in food animals has embarked upon establishing a new non-antibiotic arena in the treatment of their several infections, which ultimately not only eliminates the possibility of development of drug resistance but also render the food animals and their produce free from xenobiotic residual effects, which enter the human food chain.
Conclusion: In this review, the current knowledge on the contribution of the gut microbiota to the host well-being has been discussed. Moreover, the available information on probiotics and prebiotics and their application in animal health, production and nutrition has been reviewed.
Four littermates aged 5 years, belonging to the Dogo Argentino breed, experienced frequent regurgitation, and bilateral alopecia. The diagnostic approach of treatment was associated with a clinical examination of thoracic and abdominal... more
Four littermates aged 5 years, belonging to the Dogo Argentino breed, experienced frequent regurgitation, and bilateral alopecia. The diagnostic approach of treatment was associated with a clinical examination of thoracic and abdominal plain, using a contrast radiograph and by performing esophagogastroscopy. Skin scrapings of the affected dogs were examined microscopically to exclude any discrepancies in the observation due to parasitic infestations. The serum levels of T4, T3, and thyroid-stimulating hormone (TSH) were estimated. On the basis on medical history, clinical symptoms, reports of thyroid profile, radiologic and endoscopic findings, the dogs were diagnosed with megaesophagus concurrent with hypothyroidism. Oral administration of thyroxin and assisting feeding on a raised table was prescribed. The derma-tological signs and regurgitation improved significantly with continued feeding on.small multiple meals with a high calorie content accompanied with elevated feeding for management of megaesophagus, proton pump inhibitors Controloc ® 20 mg pantoprazole tablet per day for the long-term management of gastroesophageal reflux and gastric hyperacidity, in addition to oral levothyroxine for cure of hypothyrodism using Forthyron ® 0.8 mg pills dosed at 20 μg/kg every 12 hours for three months.
Background and Aim: Even though Ethiopia encounters considerable losses due to tick infestations , and existing research has reported the distribution and abundance of tick species across different parts of the country, there are no... more
Background and Aim: Even though Ethiopia encounters considerable losses due to tick infestations , and existing research has reported the distribution and abundance of tick species across different parts of the country, there are no evidences supporting the prevalence and distribution of hard ticks at the Horo Guduru Animal Breeding and Research Center. Therefore, the main objectives of this research are: to analyze the prevalence of ixodid ticks and identify them in the study area as well as to provide baseline data on the relative distribution of tick species.
Materials and Methods: A cross-sectional study was conducted on 409 cattle (305 local breed (Horo breed) and 104 cross breed (Horo breed × Jersey breed) using simple random sampling techniques for identification and determination of prevalence of ticks at the study area.
Results: In the present study, the overall prevalence of tick infestation was recorded as 78.23%. Two tick genera were identified with the composition of Ambylomma (51.2%) and Rhipicepha-lus (48.8%). In the study area, four tick species were identified with their respective prevalence of Rh. (B.) decoloratus (33.8%), A. variegatum (21.3%), Rh. e. evertsi (15%) and A. cohaerens (29.9%). The prevalence of tick infestation was found to be significantly different (p<0.05) between breeds with higher prevalence in local breed (Horo breed) (83.6%) than cross (Horo breed × Jersey) (62.5%). The prevalence of tick infestation among age groups was highly prevalent in adult cattles (79.08%) relative to young (73.3%) cattles. The prevalence of ticks was also highest in medium (81.4%) and lowest in good (73.4%) cattles respectively.
Conclusion: The overall prevalence rate of ixodid ticks in the current study area was 78.23%. According to the present finding, the most important tick species that was intensively investigated were A. decolaratus, Rh. evertsi-evertsi, A. varigatum, and A. cohaerence. The study indicated that there was a high burden of ticks in the discussed area.

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