... To Dr. Mamadou Baro for his guidance and willing instruction in the areas of research application. ... I would like to acknowledge Dr. Maria T. Velez of the Graduate College and Norma Maynard of the Department of Anthropology for... more
... To Dr. Mamadou Baro for his guidance and willing instruction in the areas of research application. ... I would like to acknowledge Dr. Maria T. Velez of the Graduate College and Norma Maynard of the Department of Anthropology for their time, love, prayers and unrelenting quests ...
Research Interests:
Research Interests: Sociology, Cognitive Science, Trust, Prejudice, Community Health, and 17 moreEthnicity And Health, Cultural Anthropology, Minority Health, Humans, United States, Female, Service Delivery, Data Collection, Cultural difference, African Americans, Aged, Middle Aged, Adult, Public health systems and services research, Consumer Participation, Patient Centered Care, and Level
Research Interests: Analytical Chemistry, Computational Chemistry, Quantum Chemistry, Inorganic Chemistry, Organic Chemistry, and 35 morePhysical Chemistry, Crystallography, Medicinal Chemistry, Polymer Chemistry, Reaction Mechanisms, Green Chemistry, Structure, Biosensors, Supramolecular Chemistry, Electrochemistry, Catalysis, Kinetics, Photochemistry, Spectroscopy, Mass Spectrometry, Self Assembly, Nanotechnology, Biological Chemistry, Pharmaceutical Chemistry, Surface Chemistry, Ionic Liquids, Biocatalysis, Ab initio calculations, Sensors, Scientific, Educational, Density Functional Theory, Combinatorial Chemistry, Colloids, Macromolecules, Interfaces, Proteins, Crystal Structures, BioSensors, and Combinatorial
Research Interests:
Research Interests:
Research Interests:
Cell permeable carrier peptides are currently of interest for their potential to improve the delivery of bioactive molecules into cells and to specific cellular compartments. We have investigated the activity of a derivative of the... more
Cell permeable carrier peptides are currently of interest for their potential to improve the delivery of bioactive molecules into cells and to specific cellular compartments. We have investigated the activity of a derivative of the antiandrogen drug, bicalutamide, attached to the cell-permeable carrier peptide penetratin(R). We have used both disulfide (labile) and thioether (nonlabile) linkages to attach the bicalutamide derivative to the peptide in order to assess whether one type of chemistry has advantages over the other. In addition we have added a nuclear localization sequence (NLS) to the carrier peptide to investigate whether localization of the drug to the nucleus of the cell affects the activity of the drug. Biotin-labeled peptides were used to demonstrate that the carrier peptide is rapidly accumulated inside cultured cells, and that the incorporation of an NLS in the sequence results in its nuclear targeting. The bicalutamide derivative linked to carrier peptides via a disulfide-linkage exerted no greater antiproliferative effect in LNCaP cells, than the bicalutamide derivative alone. The bicalutamide derivative linked to the carrier peptide by a non-labile thioether linkage showed a similar activity profile. When the construct includes a nuclear targeting sequence, however, a markedly increased antiproliferative effect was observed. This study has thus shown that the activity of bicalutamide may be enhanced by the nonlabile attachment of a cell-permeable and nuclear-targeted peptide, which has implications for the development of novel antiandrogens for the treatment of prostate cancer.