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  • Oceanographer from Federal University of Rio Grande - FURG (1993), master's in Ciencias del Mar from Universidad Cató... moreedit
The objective of this study was to evaluate tilapia, Oreochromis niloticus, for the biological control of suspended solids in an integrated biofloc technology (BFT) system rearing red drum, Sciaenops ocellatus, and Pacific white shrimp,... more
The objective of this study was to evaluate tilapia, Oreochromis niloticus, for the biological control of suspended solids in an integrated biofloc technology (BFT) system rearing red drum, Sciaenops ocellatus, and Pacific white shrimp, Litopenaeus vannamei. Four integrated systems were structured with recirculating water in closed circuits composed of separate tanks for each species. The experiment compared
Biofloc technology is a sustainable aquaculture production system which uses microorganisms to maintain water quality and to increase productivity. In this system, probiotics can enhance the positive effects of bioflocs on the cultured... more
Biofloc technology is a sustainable aquaculture production system which uses microorganisms to maintain water quality and to increase productivity. In this system, probiotics can enhance the positive effects of bioflocs on the cultured species. The objective of this research is to study the role of the probiotic bacterium Bacillus amyloliquefaciens during the formation of a biofloc system for the culture of Litopenaeus vannamei. Two doses of probiotic were assayed and applied directly to the water. The experiment was developed in nine tanks distributed as follows: Three control tanks with no probiotic, three tanks with a probiotic dose of 103 cfu/mL, and three tanks with a dose of 104 cfu/mL. Water quality, microbial activity, growth parameters and the immune system state of shrimps were monitored throughout the maturation process. The results indicate a positive effect upon the shrimp immune system throughout the study period, where specifically there was an increase in granular he...
The objective of this study was to evaluate tilapia, Oreochromis niloticus, for the biological control of suspended solids in an integrated biofloc technology (BFT) system rearing red drum, Sciaenops ocellatus, and Pacific white shrimp,... more
The objective of this study was to evaluate tilapia, Oreochromis niloticus, for the biological control of suspended solids in an integrated biofloc technology (BFT) system rearing red drum, Sciaenops ocellatus, and Pacific white shrimp, Litopenaeus vannamei. Four integrated systems were structured with recirculating water in closed circuits composed of separate tanks for each species. The experiment compared the use of tilapia to control suspended solids (biological treatment [BT]) to control systems without tilapia. The initial mean weights and stocking densities were: 2 g and 250 ind/m3 for shrimp; 71 g and 1.04 kg/m3 for red drum; and 85 g and 5.12 kg/m3 for tilapia. Temperature, dissolved oxygen (DO), pH, alkalinity, salinity, total suspended solids, total ammonia nitrogen (TA‐N), and nitrite were measured. Temperature, DO, pH, alkalinity, and TA‐N were maintained within guidelines recommended for shrimp, red drum, and tilapia. Nitrite concentrations remained within guidelines for red drum and tilapia. No significant difference in growth performance between the control and BT was observed, but the apparent consumption of solids in systems with tilapia resulted in decreased particulate matter in the effluent compared to the control. The study showed that the tilapia could control the suspended solids when integrated with red drum and marine shrimp culture with BFT in an integrated multitrophic system.
The constant production of solids in intensive shrimp and tilapia culture can affect the performance of macroalgae when cultivated in an integrated system, and little is known about culture structures that enhance the performance of... more
The constant production of solids in intensive shrimp and tilapia culture can affect the performance of macroalgae when cultivated in an integrated system, and little is known about culture structures that enhance the performance of macroalgae in biofloc systems. The objective of this work was to evaluate different depths of culture structure for the macroalgae Ulva lactuca in an integrated system with Litopenaeus vannamei and Oreochromis niloticus in a biofloc system. The experiment lasted 70 days, with six systems composed of: a 16 m3 shrimp tank, a 3 m3 tilapia tank, and a 3 m3 macroalgae tank, with water recirculation between tanks. Two treatments were carried out, shallow float, with a structural depth of 10 cm, and bottom float, where the depth was kept at 30 cm from the surface. The shallow float resulted in a growth rate of up to 0.95 ± 0.54% day−1, with biomass loss only at the end of the culture due to the high density of macroalgae, decreasing temperature, and increasing ...
Abstract This study evaluated the influence of different quantities of artificial substrate on water quality and the performance of Litopenaeus vannamei in an integrated biofilm-biofloc culture system. Thus, three treatments were... more
Abstract This study evaluated the influence of different quantities of artificial substrate on water quality and the performance of Litopenaeus vannamei in an integrated biofilm-biofloc culture system. Thus, three treatments were performed: the control, the treatment without the addition of artificial substrate; T200, the treatment with a 200 % increase in the lateral area of the tanks using artificial substrates; and T400, the treatment with a 400 % increase in the lateral area of the tanks using artificial substrates. The study was conducted in nine 800 L tanks over 60 days. The animals were stocked at an initial density of 300 shrimp.m−2 (equivalent to 500 shrimp m-3), with an initial weight of 1.27 g (± 0.48). Ammonia concentrations did not differ significantly between treatments (p > 0.05). Increasing the amount of substrate from 200 % to 400 % did not cause significant differences in the nitrite concentrations between these treatments. However, in the control treatment, nitrite remained high (above 20 mg.L-1) for a long period, negatively affecting shrimp performance. Nitrate was lower in T400, indicating a more dynamic process in the nitrogen cycle when the quantity of artificial substrate increased. Weekly growth rates, final weight, survival, and productivity were higher in the treatments integrating biofilm and biofloc substrates and did not show significant differences between T200 and T400. The results demonstrate the importance of artificial substrates in enhancing the water quality in biofloc culture systems over the long term, mostly in terms of maintaining nitrite concentrations below levels toxic to L. vannamei. The performance of the shrimp and the improved water quality at the end of the study reflected the advantages provided by incorporating artificial substrates in shrimp biofloc culture.
Integrating marine shrimp and tilapia has been shown to be a viable alternative in a system based on bioflocs, but there is no consensus on the spatial arrangement of farmed animals. The present study aims to (1) compare the performance... more
Integrating marine shrimp and tilapia has been shown to be a viable alternative in a system based on bioflocs, but there is no consensus on the spatial arrangement of farmed animals. The present study aims to (1) compare the performance of shrimp and fish in high density when subjected to polyculture (species in the same tank) and multitrophic (species in different tanks) arrangements, and (2) effects on water quality, especially on organic matter (biofloc). The experiment was carried out for 30 days, and three treatments with bioflocs were evaluated in triplicate: control: shrimp monoculture, polyculture: shrimp and tilapia in the same tank, and multitrophic: shrimp and tilapia in separate tanks. The results show that the best product configuration is the multitrophic system, where the biological control of bioflocs took place, and the best performance of the Pacific white shrimp L. vannamei and Nile tilapia O. niloticus was obtained. Tilapia, as an organic consumer, was effective ...
Shrimp production in biofloc systems generates excess organic matter that must be removed from the system. Due to its ability to consume natural productivity, the integration of tilapia in shrimp culture could help to reduce the levels of... more
Shrimp production in biofloc systems generates excess organic matter that must be removed from the system. Due to its ability to consume natural productivity, the integration of tilapia in shrimp culture could help to reduce the levels of total suspended solids in the biofloc system. The present study aimed to evaluate two stocking densities of tilapia in an integrated culture with shrimp Litopenaeus vannamei reared in a pilot-scale biofloc system. Two stocking densities of tilapia were tested, 35 and 65 fish m-3 in a recirculating system with 10 m3 tanks for shrimp culture and 4 m3 for tilapia culture with water recirculation of 965.66 ± 92.83 L h-1 during 78 days. The initial weight of shrimp was 0.9±0.1 g and of tilapia was 7.1±3.2 g. Shrimps were fed according to the feeding table and fish were underfed to stimulate bioflocs consumption. Selected water quality parameters were monitored during the trial. Tilapia densities did not affect shrimp growth (11.5±1.9 g and 10.1±0.7 g fo...
A biofloc system is rich in nutrients, which favors the cultivation of macroalgae, but the influence of the system on the performance of macroalgae is unknown. The objective of this study was to analyze the feasibility of introducing the... more
A biofloc system is rich in nutrients, which favors the cultivation of macroalgae, but the influence of the system on the performance of macroalgae is unknown. The objective of this study was to analyze the feasibility of introducing the macroalgae Ulva lactuca into the culture of Litopenaeus vannamei in a biofloc system. The first experiment evaluated the influence of 400 mg L−1 and 30 mg L−1 solids concentration of the system in biofloc and von Stosch culture medium on macroalgae growth. In the second experiment, the densities of 1, 2, and 3 g L−1 of U. lactuca were cultivated in an integrated system with shrimp and monoculture treatment. Both experiments had 35 days of cultivation. There was no significant difference in macroalgae growth between the treatments with biofloc and von Stosch culture medium. In the integrated culture, the density of 1 g L−1 showed better nutrient absorption. Shrimp performance was not affected by macroalgae cultivation. In conclusion, the solids did n...
Shrimp farming in Biofloc Technology System (BFT), is already considered as an alternative to the traditional culture system. The bioflocs keep the water quality and can be used as food supplement for shrimp. Most of the production in BFT... more
Shrimp farming in Biofloc Technology System (BFT), is already considered as an alternative to the traditional culture system. The bioflocs keep the water quality and can be used as food supplement for shrimp. Most of the production in BFT is based on the Pacific white shrimp Litopenaeus vannamei. However, its cultured is limited by the low temperatures. Thus, the in BFT culture potential of native species such as the pink shrimp Farfantepenaeus brasiliensis should be considered. The present study aimed to compare the culture of F. brasiliensis and L. vannamei in the Grow out phase, in BFT system. The experiment was composed of two treatments: (FB) Grow out of F. brasiliensis (LV) Grow out of L. vannamei. The study lasted 70 days and was conducted at the Marine Station of Aquaculture from Federal University of Rio Grande, Rio Grande do Sul State, Brazil. The stocking density were of 100 shrimp/m² for both species. The shrimp were fed twice a day with commercial food. Physicochemical ...
Abstract The purpose of this study was to evaluate the effect of partial harvest management on Litopenaeus vannamei reared in BFT system. The experiment lasted for 154 days and it was divided in four experimental treatments according to... more
Abstract The purpose of this study was to evaluate the effect of partial harvest management on Litopenaeus vannamei reared in BFT system. The experiment lasted for 154 days and it was divided in four experimental treatments according to the number of partial harvests. Treatment H0 had no partial harvest, H1 had one partial harvest (50% of total biomass), H2 had two partial harvests (33% of total biomass each), and H4 that had four partial harvests (20% of total biomass each). The shrimps were kept at optimal water quality conditions and were feed three times a day with commercial feed. The results of production parameters such as final weight 16.1 g (H0), 18.9 g (H1), 18.5 g (H2) and 20.1 g (H4), survival 43.67% (H0), 82.7% (H1), 86.6% (H2) and 84.7% (H4), and yield 3.48 kg.m−2 (H0), 5.85 Kg.m−2 (H1), 6.22 kg.m−2 (H2) and 6.14 kg.m−2 (H4) demonstrate significant differences among treatments. These results suggest that the partial harvest management in BFT system can leverage productivity.
This study evaluated the use of biofilm in a Litopenaeus vannamei biofloc system using different feeding rates. Shrimp juveniles (0.89 ± 0.35 g) were stocked at 300 shrimp m−3 in 24,150-L tanks. The feeding rates were calculated by... more
This study evaluated the use of biofilm in a Litopenaeus vannamei biofloc system using different feeding rates. Shrimp juveniles (0.89 ± 0.35 g) were stocked at 300 shrimp m−3 in 24,150-L tanks. The feeding rates were calculated by considering an expected weekly growth of 1 g week−1 and an estimated weekly mortality of 0.5%. Each treatment corresponded to a different feeding rate, and each feeding rate corresponded to a fixed food conversion ratio. Thus, the treatments tested were as follows: T0 and T0+B (without addition of artificial food, with and without biofilm addition, respectively); T0.6 and T0.6+B; T1.2 and T1.2+B; and T1.8 and T1.8+B. The study lasted 42 days. At the end of the study, shrimp that were grown with no artificial food presented lower final weights and minor survival, independent of the addition of biofilm. The T1.2+B treatment did not differ significantly from the T1.2, T1.8, and T1.8+B treatments for the growth and feeding parameters. The survivals were higher than 91% in all of the feed treatments, and no significant differences were detected among these treatments. In contrast, the results allowed the conclusion that the presence of biofilm in the T1.2+B treatment represented a feed saving of 35% of the total amount of artificial food offered. This could represent a significant value in the cost of operation and may make the biofloc technology (BFT) system more cost-effective and environmentally friendly. The use of BFT in conjunction with biofilm can benefit shrimp farming by reducing the amount of feed supplied.
This study analyzes the investment in Penaeus (Litopenaeus) vannamei shrimp production in greenhouses using the biofloc technology system (BFT). Considering bioeconomic variables, a sensitivity analysis was performed with data collected... more
This study analyzes the investment in Penaeus (Litopenaeus) vannamei shrimp production in greenhouses using the biofloc technology system (BFT). Considering bioeconomic variables, a sensitivity analysis was performed with data collected over the course of 10 years to evaluate program efficiency. The parameters considered include a stocking density of 400 shrimp/m2, which reached a productivity rate of 3.84 kg/m2 (69,120 kg/year—three cycles), a final mean weight of 12 g, and a feed conversion rate of 1.3. The results obtained had a net present value (NPV) of US$ 904,947.21, net future value (NFV) of US$ 2,401,094.35, equivalent annual value (EAV) of US$ 148,861.38, payback (PB) of 2 years and 4 months, discounted payback period (DPP) of 2 years and 10 months, profitability index (PI) of 2.59, internal rate of return (IRR) of 41.23%, and a modified internal rate of return (MIRR) of 21.25%. However, shrimp with a final mean weight of 15 g resulted in an NPV of US$ 2,522,150.62, NFV of US$ 6,692,016.44, EAV of US$ 414,886.98, a PB of 1 year and 2 months, DPP of 1 year and 2 months, PI of 5.43, IRR of 89.13%, and an MIRR of 30.57%. The Monte Carlo simulation (MCS) concluded that the probability of NPV being negative is 0. The analysis confirms that P. vannamei production using the BFT system is a financially sustainable activity.
In recent decades, new aquaculture technologies have been developed and improved, such as the Biofloc Technology system, which is considered an alternative to the conventional aquaculture model. This study compared the bioeconomic... more
In recent decades, new aquaculture technologies have been developed and improved, such as the Biofloc Technology system, which is considered an alternative to the conventional aquaculture model. This study compared the bioeconomic viability of intensive production in nurseries and super-intensive production of shrimp Litopenaeus vannamei bioflocs greenhouses. The investment for implementing the project was US$ 767,190.18 for intensive production and US$ 807,669.16 for super-intensive production. The analyses showed Net Present Value of US$ 363,718.21 and US$ 385,477.42, Equivalent annual value of US$ 59,830.66 and US$ 63,410.00, Net future value of US$ 965,052.69 and US$ 1,022,786.35, Payback Period 4.12 and 4.11, Discounted payback period 5.64 and 5.63, Profitability Index 1.47 and 1.48, Internal Rate of Return 20.49 and 20.55%, and Modified Internal Rate of Return 14.61 and 14.64%. The investment analysis used in this study showed that super-intensive production in a greenhouse is...
Environmental impact of semi-intensive shrimp farming on benthic fauna in southern Brazil. The increase in aquaculture activity in costal areas has raised concerns about the discharge of effluents in the aquatic environment. The aim of... more
Environmental impact of semi-intensive shrimp farming on benthic fauna in southern Brazil. The increase in aquaculture activity in costal areas has raised concerns about the discharge of effluents in the aquatic environment. The aim of this study was to determine the impact of effluents from the cultivation in Patos Lagoon - Brazil, using benthic macroinvertebrates as indicators. This research was conducted in a farm of Litopenaeus vannamei shrimp. Water parameters such as pH, diluted oxygen, oxygen consumption and total suspended solids were checked every 15 days. The sampling sites were a controlled region, the farm outflow channel and the settling ponds. The sediment for analysis of the benthic was sampled in the farm outflow channel and in the control area. One sampling occurred before the cultivation was started, and then every 15 days during the cultivation. The results show that the settling pond in shrimp cultivation was efficient. The pH and oxygen concentrations decreased,...
The aim of this study was to determine the lethal salinity (LC50) for the yellow clam Mesodesma mactroides (Bivalvia: Mesodesmatidae) and identify histopathological alterations that could be used to diagnose structural changes in clam... more
The aim of this study was to determine the lethal salinity (LC50) for the yellow clam Mesodesma mactroides (Bivalvia: Mesodesmatidae) and identify histopathological alterations that could be used to diagnose structural changes in clam tissue. Clams in two size classes (adults and juveniles) were placed in 10 L chambers and exposed to salinities of 35, 30, 25, 20, 15, 10, and 5 g/L. There were triplicate chambers with seven clams each for each salinity. The LC50 values for a 48 h exposure were 6.5 g/L and 5.7 g/L for adults and juveniles, respectively. For a 96 h exposure, the LC50 values were 10.5 g/L for adults and 8.8 g/L for juveniles. The histological examination of yellow clams exposed to 10 g/L for 96 h showed intercellular oedema and necrotic foci in the epithelium of the digestive gland and occlusion of the lumen of the digestive gland. In conclusion, M. mactroides can be characterised as a moderately euryhaline species, tolerating salinities from 35 to 15 g/L.
Abstract Currently, aquaculture seeks to implement production models that keep up with the global demands for sustainability and reduced environmental impacts. One of the options adopted is integrated multi-trophic aquaculture (IMTA),... more
Abstract Currently, aquaculture seeks to implement production models that keep up with the global demands for sustainability and reduced environmental impacts. One of the options adopted is integrated multi-trophic aquaculture (IMTA), which cultivates species of different trophic levels, improving the use of nutrients and increasing the economic profitability of the system. On the other hand, the Biofloc Technology system (BFT) has also been highlighted as an important eco-friendly activity. In an attempt to reduce total suspended solids (TSS) and in accordance with the IMTA principles, the present work evaluated the action of the oyster Crassostrea gasar on the reduction of TSS and its influence on the microbial community present on the bioflocs. An experiment with two treatments (With and Without Oyster) was carried out in waters from an ongoing marine shrimp crop. The experiment lasted for 5 days, when water samples and oyster stomach content were collected for analysis of microorganisms. The water quality parameters did not show significant differences. The TSS and aggregates number also showed no differences between treatments, indicating that the presence of oysters did not influence the amount of total suspended solids. However, the predominance of flagellates in the stomach contents of the bivalves indicates a prey selectivity by C. gasar by this microorganism. Thus, it is likely that C. gasar is not an effective tool to reduce suspended solids in IMTA, but this organism can highly benefited from protozoan present in BFT system.
Abstract The unconsumed food in shrimp farm systems is responsible for increasing the organic compounds in sediment, and it is a potential source of trace metals. This study evaluates the total organic carbon (TOC), total nitrogen (TN),... more
Abstract The unconsumed food in shrimp farm systems is responsible for increasing the organic compounds in sediment, and it is a potential source of trace metals. This study evaluates the total organic carbon (TOC), total nitrogen (TN), copper (Cu) and zinc (Zn) concentrations in sediments from control sites, a shrimp pond, a sedimentation basin and receiving ecosystem effluents at a semi-intensive Litopenaeus vannamei farm in southern Brazil. Copper and zinc concentrations were also analyzed in shrimp tissue. The sediment samples were taken before the effluent discharge (BD), 1 day post discharge (1PD), 10 days post discharge (10PD) and 30 days post discharge (30PD). The TOC concentrations ranged from 0.12 to 0.67%, and the TN concentrations were μ g g−1 and from 0.51 to 3.07 μ g g−1, respectively, while the more strongly adsorbed fraction of Cu ranged from 0.3 to 2.65 μ g g−1, and that of Zn ranged from 30.44 to 121.4 μ g g−1. Some significant differences (p ± 0.2 μ g g−1 and 19.76 ± 0.2 μ g g−1 in pond 1 and 7.6 ± 0.51 μ g g−1 and 19.13 ± 0.32 μ g g−1 in pond 2. All parameters were within in accordance with the guidelines levels, and this shrimp farm did not have adverse effects on the environment when considering these parameters.
macroinvertebrados bentonicos. ABSTRACT. Environmental impact of semi-intensive shrimp farming on benthic fauna in southern Brazil. The increase in aquaculture activity in costal areas has raised concerns about the discharge of effluents... more
macroinvertebrados bentonicos. ABSTRACT. Environmental impact of semi-intensive shrimp farming on benthic fauna in southern Brazil. The increase in aquaculture activity in costal areas has raised concerns about the discharge of effluents in the aquatic environment. The aim of this study was to determine the impact of effluents from the cultivation in Patos Lagoon - Brazil, using benthic macroinvertebrates as indicators. This research was conducted in a farm of Litopenaeus vannamei shrimp. Water parameters such as pH, diluted oxygen, oxygen consumption and total suspended solids were checked every 15 days. The sampling sites were a controlled region, the farm outflow channel and the settling ponds. The sediment for analysis of the benthic was sampled in the farm outflow channel and in the control area. One sampling occurred before the cultivation was started, and then every 15 days during the cultivation. The results show that the settling pond in shrimp cultivation was efficient. Th...
In general, the adverse effect of a chemical compound present in water varies with the concentration and time of exposure to the compound, the nature of the chemical species and age of the exposed organisms. Thus, nitrogen does not... more
In general, the adverse effect of a chemical compound present in water varies with the concentration and time of exposure to the compound, the nature of the chemical species and age of the exposed organisms. Thus, nitrogen does not necessarily cause adverse effects on shrimp, but may, instead, promote sub-lethal effects by long-term exposure. Juvenile Farfantepenaeus brasiliensis (initial mean weight = 0.61 g ± 0.07) were exposed to sub-lethal concentrations of ammonia (0.44 and 0.88 mg L-1), nitrite (5.30 and 10.60 mg L-1) and nitrate (45.60 and 91.20 mg L-1) corresponding to the safe levels for the species. After 40 days of exposure of juveniles to ammonia, nitrite and nitrate, all groups differed significantly (p<0,05) from the control group regarding the growth and survival. Based on the results, it was determined that the shrimp F. brasiliensis was susceptible to nitrogen compounds in concentrations equivalent to supposedly safe levels previously proposed for the specie. Thu...
Water quality, chlorophyll a, phytoplankton, proto and mezo-zooplankton abundance were spatiotemporally evaluated in an estuary receiving effluents from a Pacific white shrimp Litopenaeus vannamei farm in Patos Lagoon estuary, Southern... more
Water quality, chlorophyll a, phytoplankton, proto and mezo-zooplankton abundance were spatiotemporally evaluated in an estuary receiving effluents from a Pacific white shrimp Litopenaeus vannamei farm in Patos Lagoon estuary, Southern Brazil. Samples were taken before (BD) and; 1 day (1 PD) 5 days (5 PD), 10 days (10 PD), 20 days (20 PD) and 30 days (30 PD) after the effluents discharge. Some water quality parameters were affected by the effluents discharge; however, these changes were restricted to a distance of 20 m from the effluent discharge channel for a period of 5 days. The microbial community was dominated by chlorophyceae, followed by diatoms, cyanobacteria and ciliates. There was an increase in the abundance of different groups on the 1 PD sampling compared to BD. The zooplankton abundance was low in practically all sites, except for 30 PD sampling. The meso-zooplanktonic organisms were represented by copepods, mostly Acartia tonsa. Despite some effects on water quality a...
De forma general, las microalgas son fuente de alimento indispensable durante las primeras fases de desarrollo de los camarones marinos. Algunas microalgas, como la Arthrospira platensis, son ricas en substancias antioxidantes y tienen la... more
De forma general, las microalgas son fuente de alimento indispensable durante las primeras fases de desarrollo de los camarones marinos. Algunas microalgas, como la Arthrospira platensis, son ricas en substancias antioxidantes y tienen la capacidad de retirar compuestos toxicos del agua en los sistemas de produccion acuicola. De este modo, constituimos un experimento con dos tratamientos triplicados, donde se evaluo la aplicacion de biomasa filtrada de microalga A. platensis como suplemento alimenticio para camaron blanco del Pacifico, Litopenaeus vannamei. Se utilizo un sistema de cultivo estatico de seis unidades, con volumen util de 250 litros de agua marina (30 ppt), donde se sembraron 300 camarones m-2 con peso inicial de 0,7 ± 0,2 g. Al final del experimento (42 dias) se evaluaron parametros inmunologicos y de desempeno zootecnico. Los camarones alimentados con A. platensis como suplemento fueron signicativamente mayores que los camarones del tratamiento control y no presentaron diferencias en la supervivencia. Igualmente, se obtuvieron mejores resultados en los parametros inmunologicos del tratamiento con inclusion de microalga. De esta forma, al suministrar la microalga como alimento suplementario al camaron L. vannamei, crecimiento y sistema inmunologico se vieron favorecidos.

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