Biomedical papers, 2020 (vol. 164), issue 2

Gene rearrangement detection by next-generation sequencing in patients with non-small cell lung carcinoma

Aneta Brisudova, Jozef Skarda

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2020, 164(2):127-132 | DOI: 10.5507/bp.2020.015  

Gene rearrangements have been identified in patients with non-small cell lung carcinoma (NSCLC). Early detection of these biomarkers is crucial for targeted therapy determination. Currently available methods have limitations, that can be overcome by next-generation sequencing. This review compares current methods of predictive gene rearrangements detection in patients with NSCLC.

Macrophages of the subcutaneous and omental fatty tissue in obese patients: Immunohistochemical phenotyping of M2 subtypes in relation to type 2 diabetes

Jaroslava Chylikova, Jana Dvorackova, Katerina Cizkova, Helena Lacey, Vojtech Kamarad

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2020, 164(2):133-137 | DOI: 10.5507/bp.2019.011  

In this work, the authors present data on macrophage (M1/M2) phenotyping in subcutaneous and visceral fat tissues of morbidly obese patients, both with, and without, diabetes.

Measurement of novel adipokine visfatin in young patients with acute myocardial infarction. Clinical testing of a new ELISA

David Stejskal, Radka Sigutova, Marek Svestak, Jan Vaclavik, Pavlina Kusnierova, Zdenek Svagera

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2020, 164(2):138-140 | DOI: 10.5507/bp.2020.024  

The authors describe visfatin measurement in young persons with acute coronary syndrome using new techniques.

Relationship between epicardial adipose tissue thickness and coronary thrombus burden in patients with ST-elevation myocardial infarction

Abdulkadir Uslu, Ayhan Kup, Cem Dogan, Munevver Sari, Sinan Cersit, Ugur Aksu, Selcuk Kanat, Mehmet Demir, Erhan Tenekecioglu

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2020, 164(2):141-146 | DOI: 10.5507/bp.2019.038  

Determining the factors predicting thrombus burden has great importance in predicting adverse cardiovascular events as well as determining the most appropriate treatment strategy to prevent failure in PCI. EAT was found to be an independent predictor of coronary thrombus burden in STEMI.

Long-term outcome of paroxysmal atrial fibrillation catheter ablation with and without pulmonary vein dormant conduction after adenosine challenge

Tomas Skala, Jan Precek, Martin Hutyra, Ondrej Moravec, Zbynek Tudos, Jitka Skalova, Olga Klementova, Andrea Antonicka, Jana Zapletalova, Milos Taborsky

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2020, 164(2):147-153 | DOI: 10.5507/bp.2019.005  

Patients with apparently dormant conduction after adenosine during catheter ablation of paroxysmal atrial fibrillation are known to have a high arrhythmia recurrence rate. If a dormant conduction is eliminated by additional extensive ablation, these patients have the same outcome in the long term as patients without dormant conduction. This favorable outcome lasts for several years.

The efficacy of a non-leaching antibacterial central venous catheter - a prospective, randomized, double-blind study

Ivo Krikava, Martin Kolar, Barbora Garajova, Tomas Balik, Alena Sevcikova, Ingolf Roschke, Pavel Sevcik

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2020, 164(2):154-160 | DOI: 10.5507/bp.2019.022  

A prospective, randomized, double-blinded and controlled study compared non-leaching central venous catheter (Certofix® protect) with a standard catheter (Certofix®). There was no difference for catheter colonization rates between the coated and uncoated catheter. However, the coated central venous catheter showed a significantly lower incidence of bloodstream infections and a significantly lower mean incidence of bloodstream infections per 1000 catheter days.

Safe and effective percutaneous ethanol injection therapy of 200 thyroid cysts

Milan Halenka, David Karasek, Jan Schovanek, Zdenek Frysak

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2020, 164(2):161-167 | DOI: 10.5507/bp.2019.007  

Aim of this study was to highlight the possibility of using US-PEIT to treat recurrent and symptomatic thyroid cyst. In this patient cohort, the relatively small amount of alcohol (corresponding to 20% of initial volume) was sufficient to treat the cysts, with a very low incidence of complications. To our knowledge this is the third largest published patient cohort on this issue.

Monitoring of fibrinolytic system activity with plasminogen, D-dimers and FDP in primary total knee arthroplasty (TKA) after topical, intravenous or combined administration of tranexamic acid

Jiri Lostak, Jiri Gallo, Ludek Slavik, Jana Zapletalova, Lubos Balaz

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2020, 164(2):168-176 | DOI: 10.5507/bp.2019.034  

This study first describes the effect of different administration modes (topical, intravenous and combination) of tranexamic acid (TXA) on the plasma levels of plasminogen, D-dimers and FDP in patients undergoing TKA surgery. The largest antifibrinolytic effect was associated with intravenous administration of TXA. No intervention was found to significantly interfer with the process associated with systemic fibrinolysis, although D-dimers and FDPs increased over the first 12 hours after surgery and culminated around 6 hours after surgery.

Evaluation of spine structure stability at different locations during SBRT

Lukas Knybel, Jakub Cvek, Zuzana Cermakova, Jaroslav Havelka, Michaela Pomaki, Kamila Resova

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2020, 164(2):177-182 | DOI: 10.5507/bp.2019.027  

Stereotactic body radiotherapy (SBRT) requires great accuracy in order to deliver high doses of radiation to areas close to the organs at risk. SBRT of spine tumors is challenging because of the close relationship between the target tissue and the spinal cord. The present study evaluated spine motion during SBRT treatment to assess the risk of verifying patient alignment only prior to starting treatment.

Brittle cornea syndrome: Disease-causing mutations in ZNF469 and two novel variants identified in a patient followed for 26 years

Pavlina Skalicka, Louise F. Porter, Kristyna Brejchova, Frantisek Malinka, Lubica Dudakova, Petra Liskova

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2020, 164(2):183-188 | DOI: 10.5507/bp.2019.017  

Associations of cholesteryl ester transfer protein (CETP) gene variants with pituitary adenoma

Agne Sidaraite, Rasa Liutkeviciene, Brigita Glebauskiene, Alvita Vilkeviciute, Loresa Kriauciuniene

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2020, 164(2):189-195 | DOI: 10.5507/bp.2019.016  

The authors of this study tried to determine if CETP gene polymorphisms had any associations with PA. They found that rs708272 genotype G/A could be linked to a decreased risk of the PA

Downregulation of CIP2A inhibits cancer cell proliferation and vascularization in renal clear cell carcinoma

Hui Gao, Yuqiao Li, Tao Lin, Yan Cheng, Yunbo Ma

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2020, 164(2):196-202 | DOI: 10.5507/bp.2019.031  

Cancerous inhibitor of PP2A (CIP2A) was upregulated in renal clear cell carcinoma. Downregulation of CIP2A can inhibit cancer cell proliferation and vascularization in renal clear cell carcinoma through inactivation of the protein kinase B (Akt) pathway and its downstream vascular endothelial growth factor (VEGF). We characterized a novel CIP2A/Akt/VEGF in renal clear cell carcinoma.

Serum sphingosine negatively correlates with albumin predicting the risk of hepatocellular carcinoma

Kai Huang, Wanlian Li, Juan Xiao, Diteng Luo, Junfei Jin

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2020, 164(2):203-208 | DOI: 10.5507/bp.2019.015  

Serum sphingosine was increased in patients with HCC. Serum sphingosine combined with AFP provided a better result for HCC diagnosis.

Chronic calcified subdural hematoma - case report of a rare diagnosis

Pavol Snopko, Branislav Kolarovszki, Rene Opsenak, Martin Hanko, Martin Benco

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2020, 164(2):209-212 | DOI: 10.5507/bp.2019.041  

A case report of patient with unique diagnosis of chronic calcified subdural hematoma. The patient underwent successful surgical treatment - craniotomy and evacuation of subdural mass with complete resolution of preoperative neurological symptoms.

Efficacy and analgesic use during the therapy of iatrogenic pneumothorax using Pleuralvent™ and Chest Tube (ASPIRATE): A randomised controlled trial protocol

Milan Sova, Martin Poruba, Samuel Genzor, Petr Jakubec, Jaromir Zatloukal, Vitezslav Kolek, Karel Urbanek, Martina Vasakova, Ludek Stehlik, Pavla Zackova, Amjad Ghazal Asswad

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2020, 164(2):213-215 | DOI: 10.5507/bp.2020.008  

The aim of this study was to compare the efficacy of the Pleuralvent™ device and classic chest tube, in which the clinical data in the treatment of iatrogenic pneumothorax were lacking. According to the results of the study, the use of the Pleuralvent™ device could become the new standard in the treatment of iatrogenic pneumothorax.