- Erzincan, Erzincan, Turkey
Yucel Kadioglu
Ataturk University, Analytical Chemistry, Faculty Member
- Chemistry, Numerical Analysis, Analytical Chemistry, Pharmaceutical Analysis, Analytical Method Development, Pharmaceutical Analytical Chemistry, and 8 moreEnvironmental Chemistry, Chemometrics, Industrial Chemistry/Chemical Engineering, Gc-fid, Roman History, Theology and History of Vatican II, History of Roman Catholicism, and Roman Catholicismedit
- Eczacılık, Roman Catholicism, History of theology, History of Roman Catholicism, and Catholic Studiesedit
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This paper describes two rapid, sensitive and specific methods for the determination of fulvestrant in pharmaceutical preparations by high performance liquid chromatography (HPLC) and linear sweep voltammetry (LSV). HPLC method was used... more
This paper describes two rapid, sensitive and specific methods for the determination of fulvestrant in pharmaceutical preparations by high performance liquid chromatography (HPLC) and linear sweep voltammetry (LSV). HPLC method was used to study the degradation behaviour. Fulvestrant was subjected to degradation under the conditions of hydrolysis (acid and alkali), oxidation (30% H2O2). The linearity was established over the concentration range of 5-50 m g mL-1 for LSV and 0.5-20 m g mL-1 for HPLC method. The intra- and inter-day relative standard deviation (RSD) was less than 3.96 and 3.07% for LSV and HPLC, respectively. Limits of quantification were determined as 5.0 and 0.50 m g mL-1 for LSV and HPLC, respectively. No interference was found from tablet excipients at the selected assay conditions. The methods were applied for the quality control of commercial fulvestrant dosage form to quantify the drug and to check the formulation content uniformity.
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The accurate determination of prilocaine HCl levels in plasma is important in both clinical and pharmacological/toxicological studies. Prilocaine HCl is quickly hydrolyzed to o-toluidine, causing methemoglobinemia. For this, the present... more
The accurate determination of prilocaine HCl levels in plasma is important in both clinical and pharmacological/toxicological studies. Prilocaine HCl is quickly hydrolyzed to o-toluidine, causing methemoglobinemia. For this, the present work describes the methodology and validation of a GC-MS assay for determination of prilocaine HCl with lidocaine HCl as internal standard in plasma. The validation parameters of linearity, precision, accuracy, recovery, specificity, limit of detection and limit of quantification were studied. The range of quantification for the GC-MS was 20-250 ng/mL in plasma. Within-day and between-day precision, expressed as the relative standard deviation (RSD) were less than 6.0%, and accuracy (relative error) was better than 9.0% (n = 6). The analytical recovery of prilocaine HCl and IS from plasma has averaged 94.79 and 96.8%, respectively. LOQ and LOD values for plasma were found to be 20 and 10 ng/mL, respectively. The GC-MS method can be used for determination from plasma of prilocaine HCl in routine measurement as well as in pharmacokinetic studies for clinical use.
Research Interests: Chemistry, Analytical Chemistry, Chromatography, Analytical Method Development, Medicine, and 11 moreHumans, Calibration, Gas Chromatography, Lidocaine, Gas Chromatography/mass Spectrometry, Reproducibility of Results, Mass Spectroscopy, Sensitivity and Specificity, Prilocaine, Reference standards, and Pharmacology and pharmaceutical sciences
Erectile dysfunction (ED) diseases have almost affected 100 million men all over the world. Orally administered phosphodiesterase 5 (PDE 5) inhibitors are the most used pharmaceutical formulations for the treatment of ED. In this study,... more
Erectile dysfunction (ED) diseases have almost affected 100 million men all over the world. Orally administered phosphodiesterase 5 (PDE 5) inhibitors are the most used pharmaceutical formulations for the treatment of ED. In this study, it is aimed to investigate the metabolomics feature of orally administered vardenafil in rats. To carry out the experimental procedure eight male Wistar albino rats were used. Their livers were gently removed and metabolomics profiles of each sample were determined by UPLC Q-TOF MS. Identification of metabolites was achieved by the METLIN database. Cluster analysis was also performed via Principle Component Analysis. Several metabolites were identified and results were evaluated by XCMS software. UPLC Q-TOF MS could be successfully applied to profile biomarkers and help us understand the molecular mechanisms of vardenafil usage. It was concluded that the level of some metabolites, responsible for the collagen synthesis and Kreb's cycle, has been statistically significant after the vardenafil administration.
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ABSTRACT This study aimed to review recent chromatographic methods for quantifying and identifying components released from dental composites. Resin-based dental restorative materials are extensively used in dentistry today. Although... more
ABSTRACT This study aimed to review recent chromatographic methods for quantifying and identifying components released from dental composites. Resin-based dental restorative materials are extensively used in dentistry today. Although composite materials are known to be highly stable structures, they are susceptible to degradation because of the incomplete polymerization. Several components may be released from resin composite restorations into the oral environment. The elution of components from composite resins may affect the biocompatibility of the restorations. Therefore, it is essential to understand the nature and quantity of substances that are segregated into the oral cavity. GRAPHICAL ABSTRACT
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A new spectrofluorimetric method to determine losartan potassium (LP) in rabbit plasma is described. The method was based on measuring the native fluorescence of LP in acidic medium. Optimum excitation and emission wavelengths were found... more
A new spectrofluorimetric method to determine losartan potassium (LP) in rabbit plasma is described. The method was based on measuring the native fluorescence of LP in acidic medium. Optimum excitation and emission wavelengths were found to be 248 nm and 410 nm, respectively, in methanol that was diluted with a sulfurous acid solution LP was extracted from rabbit plasma by methyl-tertiary-butyl-ether in acidic media and then back extracted with NaOH. The calibration curves were linear between 0.025 and 0.5 µg/mL with a lower limit of detection 0.004 µg/mL. Precision and accuracy values of the method were calculated as lower than 4.97% and ± 5.68, respectively and the recovery of LP from rabbit plasma was higher than 91.1%. In addition, stability studies of LP in rabbit plasma were carried out and demonstrated its good stability at - 20 °C and at room temperature. The developed and validated method was successfully applied for estimating the pharmacokinetic parameters of LP following oral administrations of a single 10 mg LP/kg to rabbits and it could be concluded that the method can be applied to clinical trials.
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Invasive ductal carcinoma (IDC) is the most common type of breast cancer. In this study, matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS)-based analyses were conducted for determining differential N-glycosylation... more
Invasive ductal carcinoma (IDC) is the most common type of breast cancer. In this study, matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS)-based analyses were conducted for determining differential N-glycosylation patterns of IDC.
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Research Interests: Chemistry, Analytical Chemistry, Chromatography, Medicine, Calibration, and 12 moreSpectrophotometry, Aspirin, Solubility, Ibuprofen, Ascorbic Acid, Pyridoxine, Micellar Liquid Chromatography, Acetaminophen, Pseudoephedrine, Tyrosine, Pyridoxine Hydrochloride, and Pharmacology and pharmaceutical sciences
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Research Interests: Chemistry, Analytical Chemistry, Chromatography, Biological Sciences, Calibration, and 15 moreAnimals, Analytical, Acute Pancreatitis, CHEMICAL SCIENCES, Cattle, Aged, Adult, Clinical Study, AAPS PharmSciTech, Clinical Application, Biochemistry and cell biology, Butylated hydroxytoluene, Acute Disease, Dicloxacillin, and canagliflozin
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For the quantification of flurbiprofen in rat plasma, a simple UPLC-MS/MS method with high sensitivity and short retention time for flurbiprofen was developed and validated using specific parameters. Etodolac was used as internal... more
For the quantification of flurbiprofen in rat plasma, a simple UPLC-MS/MS method with high sensitivity and short retention time for flurbiprofen was developed and validated using specific parameters. Etodolac was used as internal standard. The transitions (precursor to the product) of flurbiprofen and internal standard were obtained using the electrospray ionization in the negative ion multiple reaction monitoring mode, 243.2 → 199.2, 286.2 → 212.1, respectively. For chromatographic separation, C18 column was used for the stationary phase and gradient elution was used for the mobile phase. This mobile phase consisted of a methanol (A) and a 5 mM ammonium formate solution (B), which varied at a flow rate of 0.4 mL/min. For flurbiprofen, LLOQ was determined as 5 ng/mL. Quantification of flurbiprofen in the rat plasma with a linear calibration curve of 5–5000 ng/mL (r > 0.9991 for plasma) is possible with a retention time of 1.89 min. The total analysis time of the method was 3 min....
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Prilocaine is a local anaesthetic compound and methemoglobinemia may occur after its administration. In some cases, it may lead to dyspnoea, dysrhythmia, seizures and coma. Chitosan (CS) nanoparticles are potential vehicles for controlled... more
Prilocaine is a local anaesthetic compound and methemoglobinemia may occur after its administration. In some cases, it may lead to dyspnoea, dysrhythmia, seizures and coma. Chitosan (CS) nanoparticles are potential vehicles for controlled delivery of drugs and have the capacity to protect sensitive bioactive materials from enzymatic and chemical degradation in vivo. The purpose of this study was to prepare and characterize prilocaine-loaded CS nanoparticles and evaluate their potential for reducing side effects in local anaesthetic applications. Prilocaine-loaded CS nanoparticles are evaluated for their particle size, zeta potential, drug loading capacity and release properties. Quantification of the prilocaine was carried out with a validated gas chromatography method. According to the data obtained, these chitosan-based prilocaine nanoparticles May open an interesting perspective for localised delivery of prilocaine, thus reducing side effects
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Ultra-high-performance liquid chromatographic UPLC data obtained from photodiode array PDA detection was processed by the PCR and PLS algorithms for the simultaneous quantitative resolution of Ascorbic Acid AA , Paracetamol PAR , Aspirin... more
Ultra-high-performance liquid chromatographic UPLC data obtained from photodiode array PDA detection was processed by the PCR and PLS algorithms for the simultaneous quantitative resolution of Ascorbic Acid AA , Paracetamol PAR , Aspirin ASP in a commercial formulation. Principle component regression PCR and partial least squares PLS were applied to the peak area ratio of the analytes/internal standard at multi-wavelength PDA detector responses. The combined use of UPLC and chemometric calibration techniques was denoted UPLC- PCR and UPLC-PLS. For the comparison purpose, the UPLC method was used for the confirmation of the results obtained from combined UPLC-chemometric calibration techniques. A good chromatographic separation between drugs and internal standard IS was achieved using a Waters ACQUITY UPLC BEH Phenyl 100 mm x 1.0 mm, i.d., 1.7 μm column and a mobile phase consisting of 0.1 M CH3COOH and methanol 75:25, v/v . The multi-wavelength PDA detection for Ascorbic Acid AA , P...
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ABSTRACT The dissolution kinetics of natural pyrite samples in Cl2-saturated carbon tetrachloride and water-carbon tetrachloride media have been investigated. The effects of pyrite particle size, reaction time, temperature, stirring speed... more
ABSTRACT The dissolution kinetics of natural pyrite samples in Cl2-saturated carbon tetrachloride and water-carbon tetrachloride media have been investigated. The effects of pyrite particle size, reaction time, temperature, stirring speed and water-to-carbon tetrachloride ratio were studied. The dissolution rate of pyrite in these media increased with increase in temperature and decrease in particle size. In water-carbon tetrachloride medium, the rate was independent of the stirring speed. However, in carbon tetrachloride medium alone, the increase in the stirring speed significantly increased the dissolution rate. The experimental data were analyzed on the basis of unreacted shrinking core model. It has been found that the dissolution is controlled by diffusion through the ash layer in carbon tetrachloride and that it is chemically-controlled in water-carbon tetrachloride medium. The activation energies for the diffusion- and the chemically-controlled steps were 32.3 and 22.3 kJ mol−1, respectively.
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This study evaluated the spontaneous combustion characteristics of Askale lignite from Turkey. The effect of the gas flow rate, the moisture of the piles of coal, the humidity of the air and particle size on the spontaneous combustion... more
This study evaluated the spontaneous combustion characteristics of Askale lignite from Turkey. The effect of the gas flow rate, the moisture of the piles of coal, the humidity of the air and particle size on the spontaneous combustion characteristics of coal samples were examined using Crossing Point Methods adapted to our laboratories conditions. The amounts of three predominant oxygen functional
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Abstract The present work describes the methodology and validation of gas chromatography with flame ionization detection (GC-FID) for the determination of carbamazepine with internal standard (diazepam) in pharmaceutical preparations. The... more
Abstract The present work describes the methodology and validation of gas chromatography with flame ionization detection (GC-FID) for the determination of carbamazepine with internal standard (diazepam) in pharmaceutical preparations. The method was linear from ...
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Research Interests: Mathematics, Applied Mathematics, Analytical Chemistry, Chemometrics, Principal Component Analysis, and 12 moreWavelet, Quantitative analysis, Wavelet Transform, Partial Least Squares, Partial Least Squares Regression, Nonlinear Science, Numerical Analysis and Computational Mathematics, Quantitative Analysis, Absorbance, Absorption Spectra, Partial Least Square, and Principal Component Regression
A simple, sensitive, precise, accurate and specific HPLC method for the determination of CBZ in human plasma was developed and validated. The CBZ was isolated from human plasma by liquid-liquid extraction, gave good results with... more
A simple, sensitive, precise, accurate and specific HPLC method for the determination of CBZ in human plasma was developed and validated. The CBZ was isolated from human plasma by liquid-liquid extraction, gave good results with chloroform. The method was linear in the concentration range 0.5 to 25 μg ml-1 (r>0.998). Recovery for CBZ was greater than 91.7 %. The limit of quantification (LOQ) and the limit of detection (LOD) were 0.15 μg ml-1 and 0.10 μg.ml-1, respectively. Inter-batch precision, expressed as the relative standard deviation (RSD), ranged from 0.79 to 6.30 % for intra day and from 1.60 to 1.95 % for inter day and accuracy was better than 90 %. Analysis of CBZ concentrations in plasma samples from six healthy volunteers following oral administration of single dose of CBZ (Tegretol i£¨ CR 200 (200 mg)) provided the following pharmacokinetic data (mean ± SD): Cmax, 1.550±0.468 μg ml-1; AUC0-72 , 52.190±18.942 μg h mL-1; AUC0- 8, 68.306±21.698 μg h ml-1; Tmax, 6,120±6,632 h; t½ 34.270±16.787 h; CL, 3.2602 l min-1.
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The aim of this study was to develop and verify a simple, rapid and sensitive high performance liquid chromatography method coupled with UV detector HPLC UV method for the quantitative determination of etodolac in bulk and pharmaceutical... more
The aim of this study was to develop and verify a simple, rapid and sensitive high performance liquid chromatography method coupled with UV detector HPLC UV method for the quantitative determination of etodolac in bulk and pharmaceutical dosage forms. Chromatographic separation was performed at ambient conditions on a reverse phase ACE C8 analytical column 250 mm x 4.6 mm ID, 5 umm using the mobile phase containing acetonitrile water 80 20, v v at a flow rate of 1.0 mL min 1. A wavelength of 272 nm was used for etodolok and paracetamol IS . A retention time of 4.21 min and 2.02 min were obtained for etodolac and IS, respectively. The method showed linearity in the range of 0.08 10 µg mL 1 for etodolac R = 0.9999 . The linear regression equations obtained by least square regression method were the ratio of peak area of etodolac and IS =1.559 concentration etodolac µg mL 0.139. The intra day and inter day RE and RSD values of the method were =10.0 and =2.65 , respectively. Limit of de...
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A simple and sensitive spectrophotometric method was developed for the determination of prilocaine HCl in pharmaceutical preparation and human plasma. The quantitative analysis of prilocaine HCl was carried out using of wavelength at 230... more
A simple and sensitive spectrophotometric method was developed for the determination of prilocaine HCl in pharmaceutical preparation and human plasma. The quantitative analysis of prilocaine HCl was carried out using of wavelength at 230 nm. The method was linear in concentration range of 3-15 μg/mL for standard solution and 4-15 μg/mL for human plasma. Linearity was determined by calculating correlation coefficient. These values were found as a 0.9999 and 0.9967 in standard solution and human plasma, respectively. Developed spectrophotometric method was found suitable in terms of accuracy, sensitivity, precision, reproducibility. In addition to these, this method could be easily and directly applied to both human plasma and pharmaceutical preparation. INTRODUCTION: The reason of using local anesthetics is to block the conduction of impulses in nerve fibers which cause anesthesia. They are most commonly used in dentistry and minor surgery in order to provide
The rapid and sensitive UV spectrophotometric method was described for the determination of etodolac, non-steroidal antiinflammatory drugs. The type of solvent, the range of wavelength and the range of concentration were chosen in order... more
The rapid and sensitive UV spectrophotometric method was described for the determination of etodolac, non-steroidal antiinflammatory drugs. The type of solvent, the range of wavelength and the range of concentration were chosen in order to optimize the conditions. The developed and validated method was successfully applied to the analysis of etodolac without derivatization with any compound in commercial formulations. The standard calibration curve of etodolac was constructed by plotting absorbance versus concentration in the determined concentration range with the final dilution. The calibration curve was Absorbance in 277 nm wavelength = 0.0303 Concentration in ppm –0.0201 and linear (R0.9995) in concentration range of 1-50 ppm of etodolac. The LOD and LOQ values of method were 0.7 ppm and 1.0 ppm, respectively. Moreover, the proposed method was fast with respect to analysis time as compared to more sophisticated chromatographic techniques. INTRODUCTION: Non-steroidal antiinflamm...
Firstand second-order derivative spectrophotometric methods for the quantitative determination by converting menadione sodium bisulphite (MSB) to menadione (M ) with sodium carbonate were developed and validated in pharmaceutical... more
Firstand second-order derivative spectrophotometric methods for the quantitative determination by converting menadione sodium bisulphite (MSB) to menadione (M ) with sodium carbonate were developed and validated in pharmaceutical preparation. The quantitative determination of MSB was carried out by using wavelengths 350 nm (for first-order; 1D) and 355 nm (for second-order; 2D) with the linearity ranges 0.5-40 μg/mL M (i.e.0.82-65.6 μg/mL MSB). The percentages of conversion MSB to M obtained with methods were more than 97.1 %. The proposed derivative spectrophotometric methods easily applied to commercial ampoule containing MSB and were statistically compared.
Zero, first and second order derivative spectrophot ometric methods were developed for the determination of insulin. The standard solutions and pharmaceutical sample were p repared in 0.01 M HCl. Absorbences of insulin were measured at... more
Zero, first and second order derivative spectrophot ometric methods were developed for the determination of insulin. The standard solutions and pharmaceutical sample were p repared in 0.01 M HCl. Absorbences of insulin were measured at 276 nm for the zero order by measuring height of pe ak from zero, at 264, 277 and 284 nm for the first order and at 277, 280, 283 and 289 nm for second order derivative spe ctrophotometric method by measuring peak to peak height. The type of solvent, the degree of deviation and range of wavel ength were chosen in order to optimize the conditio ns. The linearity ranges were found to be 5.0-70 µg mL -1 for the zero, first and second order derivative spe ctrophotometric methods. The detection and quantitation limits were found to be 2.0 µg mL -1 & 3.0 µg mL -1 for the zero order, 3.0 µg mL -1 & 4.0 µg mL -1 for first and second order derivative spectrophotom etric methods, respectively. The developed zero, fi rst and second order derivative spectrophotom...
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Olanzapine which is named as 2-methyl-4-4-(4-methyl-1-piperazynyl)-10H-thio (2,3-b)(1,5) benzodiazepine is used as a pharmaceutical formulation since 1985. Olanzapine is an atypical antipsycotic drug that has a great affinity to dopamine... more
Olanzapine which is named as 2-methyl-4-4-(4-methyl-1-piperazynyl)-10H-thio (2,3-b)(1,5) benzodiazepine is used as a pharmaceutical formulation since 1985. Olanzapine is an atypical antipsycotic drug that has a great affinity to dopamine (D1, D2, D3, D4), serotonine (5HT-2A, 5HT-2C, 5HT3, 5HT6), muskarinic (M1, M5) histamine H1 and 1 adrenejenik receptors. By this way it can differenttiated from the unselective typical antipsycotics which block the both stratial and limbic neurons and cause adverse effects. In this study, it is aimed to develop and validate an accurate, precise, sensitive LC-MS method to determine olanzapine in pharmaceutical formulations. In order to carry out this study, method parameters were optimized to be 1 mL/min flow rate, 25°C column temperature, 0.1% TFA-acetonitrile (20:80 v/v) mobile phase, positive ion mode with 313 m/z ratio, 100 V fragmentor voltage, 5μA corona current and Agilent C18 column (5 μm, 150 x 4,6 mm). The proposed method were validated wit...
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A new method was developed for the determination of 17 β-estradiol in rabbit plasma by reversed-phase high performance liquid chromatography (RP-HPLC) with fluorescence (FL) detection. The method employed one-step extraction of 17... more
A new method was developed for the determination of 17 β-estradiol in rabbit plasma by reversed-phase high performance liquid chromatography (RP-HPLC) with fluorescence (FL) detection. The method employed one-step extraction of 17 β-estradiol from plasma matrix with a mixture of water and ethylacetate (1.9:5, v/v) using estriol as an internal standard. The mobile phase consisted of a mixture of metanol and water (70:30, v/v) flowing at a flow rate of 1.0 mL min−1. Calibration curve was linear between concentration range of 125–6000 ng mL−1. Average recovery of 17 β-estradiol and the internal standard from the biological matrix was more than 94.9 and 92.5%, respectively. The intra-day and inter-day precision and accuracy were between 3.74–8.12 and 3.72–8.80%, respectively. Also, the method was successfully applied to determine of 17 β-estradiol in New Zealand white rabbits.
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Abstract In this study, new, rapid UV spectrophotometry (UV) and reversed phase high performance liquid chromatography (HPLC) methods were developed for the determination of 17 β-estradiol in pure and in pharmaceutical dosage form. The... more
Abstract In this study, new, rapid UV spectrophotometry (UV) and reversed phase high performance liquid chromatography (HPLC) methods were developed for the determination of 17 β-estradiol in pure and in pharmaceutical dosage form. The solvent system, wavelength of detection and chromatographic conditions were optimized in order to maximize the sensitivity of both the proposed methods. The linear regression equations obtained by least square regression method were y = 0.0184x + 0.0059 for the UV method and y = 56742x − 3403.6 for the HPLC method. The developed methods were successfully employed with a high degree of precision and accuracy for the estimation of total drug content in a commercial tablet of 17 β-estradiol. The results obtained from the UV method were compared with those obtained by using HPLC. The proposed methods are highly sensitive, precise and accurate and can be used for the reliable quantitation of 17 β-estradiol in pharmaceutical dosage form.
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An HPLC system using a new, simple and rapid liquid-liquid extraction and high-performance liquid chromatography-diode array detector method (HPLC-DAD) detection was validated to determine tramadol concentration in rabbit plasma. The... more
An HPLC system using a new, simple and rapid liquid-liquid extraction and high-performance liquid chromatography-diode array detector method (HPLC-DAD) detection was validated to determine tramadol concentration in rabbit plasma. The method described was applied to a pharmacokinetic study of intravenous tramadol injections in rabbits. The extraction with ethylacetate yielded good response. The recovery of tramadol from plasma averaged 90.40%. Serial plasma samples were obtained prior to, during and after completion of the infusion for determination of tramadol concentrations. Tramadol concentrations were measured using reverse-phase high-performance liquid chromatography and pharmacokinetic application with intravenous tramadol in rabbits revealed that tramadol followed one-compartment open model. Maximum plasma concentration (C(max)) and area under the plasma concentration-time curve (AUC) for tramadol were 14.3 microg mL(-1) and 42.2 microg h mL(-1), respectively. The method developed was successfully applied to a simple, rapid, specific, sensitive and accurate HPLC method for investigation of the pharmacokinetics of tramadol in rabbit plasma.