Wombats

In this review we investigated the diet and nutrition of wombats and highlight areas for future research. The low energy requirements of wombats, conservative foraging behaviours and burrowing lifestyle allow them to subsist on low-quality food. Furthermore, their digestive anatomy and physiology is well adapted for digesting the resources available in their habitats. All species feed predominantly on native grasses; however, the nutritional requirements of juvenile and aged wombats are largely unknown. Changes to food availability and grass species in wombat habitats may alter their diet and nutritional intake. Also, despite evidence suggesting that wombats can satisfy their water requirements via the food they eat, the minimum threshold of water content at which wombats will cease feeding is unknown. Other areas for future research include investigating factors that affect digesta retention times (such as age or lactation), and the development of a method to determine age, because...

Wombats are large marsupial grazers native to Australia. They are 'ecosystem engineers' due to their burrowing, impacting rates of soil turnover, assisting with nutrient cycling and water infiltration, and creating habitat for other species. The aim of this review is to provide an overview of available information on the habitat and distribution, abundance and threats to bare-nosed wombats (Vombatus ursinus), and to provide a critical review of the current gaps in our knowledge to aid future management of the species. Bare-nosed wombats were previously widespread. Since European settlement their range has reduced. While habitat loss and contraction impact population numbers, bare-nosed wombat populations are also threatened by roadkill, introduced species, legal and illegal human persecution, and disease. Further research is required to understand the historic range of bare-nosed wombats, as well as current population numbers, so that we can determine the threats that most significantly impact this species. Increasing our understanding of habitat requirements, distribution and population numbers, as well as how to mitigate threats, will aid future management and conservation strategies for the species.

Roadkill is a worldwide issue that can cause local population extinctions. In Australia numerous species are affected, however the bare-nosed wombat (Vombatus ursinus) is particularly vulnerable due to its preference for disturbed habitats. Collisions with motor vehicles causes significant damage to the vehicle and sometimes the driver, as well as wounding or killing the wombat. Virtual fences are light and sound-based devices, originally developed in Austria that can be used to reduce roadkill through mitigation. They have had mixed results. In this study a virtual fence was installed along a 1.5 km stretch of road in semi-rural NSW, Australia, with the aim of reducing wombat roadkill through the use of this form of mitigation strategy. The number of roadkilled wombats was counted before and after the fence was installed in March 2020. Prior to the fence being installed 23 wombats were killed and after the fence was installed six wombats were killed. Along Old Bega Road, outside of the fenced area 64 wombats were killed pre-fence installation and 17 post fence installation. Bare-nosed wombats are semi-fossorial ecosystem engineers with an important role in the ecosystem and despite being listed as Least Concern are readily impacted by roadkill. Virtual fencing implemented in regions that have high wombat roadkill rates may aid in reducing road deaths and species conservation. However, we recommend that more research is required to assess virtual fencing, as a roadkill mitigation strategy, including an investigation into a larger number of species in a range of different habitats.

This study contrasts the actual conservation spending and the Australian public’s demand for conservation funding for two Australian mammal species, the koala and the northern hairy-nosed wombat. It involves a survey of 204 members of the Australian public. Willingness to fund conservation action to protect the northern hairy-nosed wombat was found to be higher than that for the koala despite the koala’s immense popularity. The critically endangered status of the northern-hairy nosed wombat and the more secure conservation status of the koala is a factor likely to have influenced the comparative willingness-to-pay decisions. Actual annual conservation expenditure for both species is lower than the estimated aggregate willingness-to-pay for their conservation. Furthermore, conservation funding for the koala is much more than that for the northern hairy-nosed wombat even though the estimated public willingness-to-pay (demand) for funding koala conservation was less than for this wombat species. Reasons for this are suggested. They may also help to explain misalignment between demand for conservation funding of other species involving differences in charisma and endangerment.

Ticks (Arachnida: Acari) are vectors for pathogens and the biggest threat to animal health. Many Australian ticks are associated with pathogens that impact humans, domestic animals and livestock. However, little is known about the presence or impact of tick-borne pathogens in native Australian wildlife. Wombats are particularly susceptible to the effects of the ectoparasite Sarcoptes scabiei which causes sarcoptic mange, the reason for which is unknown. Factors such as other ectoparasites and their associated pathogens may play a role. A critical understanding of the species of ectoparasites that parasitise wombats and their pathogens, and particularly ticks, is therefore warranted. This review describes the ectoparasites of wombats, pathogens known to be associated with those ectoparasites, and related literature gaps. Pathogens have been isolated in most tick species that typically feed on wombats; however, there are minimal molecular studies to determine the presence of pathogens in any other wombat ectoparasites. The development of next-generation sequencing (NGS) technologies allows us to explore entire microbial communities in ectoparasite samples, allowing fast and accurate identification of potential pathogens in many samples at once. These new techniques have highlighted the diversity and uniqueness of native ticks and their microbiomes, including pathogens of potential medical and veterinary importance. An increased understanding of all ectoparasites that parasitise wombats, and their associated pathogens, requires further investigation.

Experimental surveys were used to gather evidence on the likeability of the focal species, the stated willingness of survey participants to contribute funds for the conservation of species and the impact of information pro­ vision on these factors. Information was given to participants to increase their knowledge about the focal species, including their conservation status.
After providing some general background on the focal species, the methodology is outlined. The willingness to pay for the conservation of the species (the demand results) obtained from the experimental survey is then reported. This is followed by an estimate of actual expenditures in Queensland (at the time of the surveys) on the conservation of koalas and hairy-nosed wombats and these are compared with the estimated willingness-to-pay figures. Discrepancies are observed and then discussed and possible limitations of the study are outlined.

Archaeologists and palaeoecologists are increasingly turning to stable isotope analysis (δ 13 C, δ 18 O) of fossil bioapatite to examine interactions of human and animal populations. However, relatively few investigations have focussed on the identification of natural variation in comparable modern populations, particularly within the Australian context. In this paper, we present the first modern isotopic reference dataset for Tasmanian bare-nosed wombat teeth (Vombatus ursinus tasmaniensis). Samples for δ 13 C bioapatite and δ 18 O bioapatite measurements were recovered sequentially at sub-monthly resolution from all tooth types. δ 13 C bioapatite showed little variation within a seasonal sinusoidal pattern within the sample set (n = 24 wombats; 35 teeth) due to the homogeneous C 3 distribution of plants in Tasmania. In contrast , δ 18 O bioapatite profiles varied seasonally, representing time periods of between 0.9 and 2.1 years in 95% of the sample. Significant differences between tooth types were found from intra-individual to interregional scales for both dental growth rates and isotopic values. The accuracy of season-of-death assessments differed across the island; those in eastern Tasmania were accurate in all instances whereas those in the west showed substantial inaccuracies. We suggest that this may be due to the elodont form of wombat dentition and the ecologically influenced seasonally varied diet in western Tasmania. As the rate of dental growth is positively correlated with the proportion of coarse vegetation within the diet, this seasonal variation is therefore likely to change how annual isotopic signals are incorporated into the enamel. These results highlight the need to understand the degree of species-specific isotopic variation at a range of scales before applying this technique to archaeological or palaeontological assemblages.

Background: Ticks are obligate haematophagous ectoparasites of vertebrate hosts and transmit the widest range of pathogenic organisms of any arthropod vector. Seven tick species are known to feed on bare-nosed wombats (Vom-batus ursinus), in addition to the highly prevalent Sarcoptes scabiei mite which causes fatal sarcoptic mange in most bare-nosed wombat populations. Little is known about the pathogens carried by most wombat ticks or how they may impact wombats and wombat handlers. Methods: Wombat ticks were sourced from wildlife hospitals and sanctuaries across Australia and identified to species level using taxonomic keys. Genomic DNA was extracted from a subsample, and following the amplification of the bacterial 16S rRNA gene V3-V4 hypervariable region, next-generation sequencing (NGS) on the Illumina MiSeq platform was used to assess the microbial composition. Results: A total of 447 tick specimens were collected from 47 bare-nosed wombats between January 2019 and Janu-ary 2020. Five species of ticks were identified comprising wombat tick Bothriocroton auruginans (n = 420), wallaby tick Haemaphysalis bancrofti (n = 8), bush tick Haemaphysalis longicornis (n = 3), common marsupial tick Ixodes tasmani (n = 12), and Australian paralysis tick Ixodes holocyclus (n = 4). Tick infestations ranged from one to 73 ticks per wombat. The wombat tick was the most prevalent tick species comprising 94% of the total number of samples and was present on 97.9% (46/47) of wombat hosts. NGS results revealed the 16S rRNA gene diversity profile was predominantly Proteobacteria (55.1%) followed by Firmicutes (21.9%) and Actinobacteria (18.4%). A species of Coxiella sharing closest sequence identity to Coxiella burnetii (99.07%), was detected in 72% of B. auruginans and a Rickettsiella endosymbiont dominated the bacterial profile for I. tasmani. Conclusions: A new host record for H. longicornis is the bare-nosed wombat. One adult male and two engorged adult female specimens were found on an adult male wombat from Coolagolite in New South Wales, and more specimens should be collected to confirm this host record. The most prevalent tick found on bare-nosed wombats was B. auruginans, confirming previous records. Analysis of alpha-diversity showed high variability across both sample locations and instars, similar to previous studies. The detection of various Proteobacteria in this study highlights the high bacterial diversity in native Australian ticks.

Background Wombats are large, nocturnal herbivores that build burrows in a variety of habitats, including grassland communities, and can come into conflict with people. Counting the number of active burrows provides information on the local distribution and abundance of wombats and could prove to be an important management tool to monitor population numbers over time. We compared traditional ground surveys and a new method employing drones, to determine if drones could be used to effectively identify and monitor bare-nosed wombat burrows. Results We surveyed burrows using both methods in eight 5-ha transects in grassland, that was interspersed with patches of tussock grassland. Ground surveys were conducted by systematically walking transects and searching for burrows. Drone surveys involved programming flights over transects to capture multiple images, from which an orthomosaic image of each transect was produced. These were subsequently viewed using ArcMap to detect burrows. A tot...

Ticks (Arachnida: Acari) are vectors for pathogens and the biggest threat to animal health. Many Australian ticks are associated with pathogens that impact humans, domestic animals and livestock. However, little is known about the presence or impact of tick-borne pathogens in native Australian wildlife. Wombats are particularly susceptible to the effects of the ectoparasite Sarcoptes scabiei which causes sarcoptic mange, the reason for which is unknown. Factors such as other ectoparasites and their associated pathogens may play a role. A critical understanding of the species of ectoparasites that parasitise wombats and their pathogens, and particularly ticks, is therefore warranted. This review describes the ectoparasites of wombats, pathogens known to be associated with those ectoparasites, and related literature gaps. Pathogens have been isolated in most tick species that typically feed on wombats; however, there are minimal molecular studies to determine the presence of pathogens...

Wombats are large marsupial grazers native to Australia. They are ‘ecosystem engineers’ due to their burrowing, impacting rates of soil turnover, assisting with nutrient cycling and water infiltration, and creating habitat for other species. The aim of this review is to provide an overview of available information on the habitat and distribution, abundance and threats to bare-nosed wombats (Vombatus ursinus), and to provide a critical review of the current gaps in our knowledge to aid future management of the species. Bare-nosed wombats were previously widespread. Since European settlement their range has reduced. While habitat loss and contraction impact population numbers, bare-nosed wombat populations are also threatened by roadkill, introduced species, legal and illegal human persecution, and disease. Further research is required to understand the historic range of bare-nosed wombats, as well as current population numbers, so that we can determine the threats that most significa...

Studies of gene diversity are used to investigate population dynamics, including immunological fitness. Aside from the selection of an appropriate gene target, an important factor that underpins these studies is the ability to recover viable DNA samples from native animals that are protected, threatened or difficult to sample or locate such as the bare-nosed wombat (Vombatus ursinus). In this study, we used genomic DNA extracted from muscle tissue samples and also identified the optimal method to extract DNA from fresh wombat scat samples to enable further analyses to be performed using non-invasive techniques. The DNA was probed via the polymerase chain reaction using previously targeted marsupial Major Histocompatibility Complex (MHC) gene primers. These genes are highly variable and associated with binding and presentation of pathogens in the immune system. Twenty-three unique MHC Class II DAB V. ursinus gene sequences were translated to 21 unique predicted peptide sequences from 34 individual tissue or scat samples. Vombatus ursinus MHC Class II DAB gene and peptide sequences were most similar to other marsupial DNA and peptide sequences. Further analysis also indicated the likelihood of MHC Class II DAB family membership through motif identification. Additional sampling is required to assess the full level of diversity of MHC Class II DAB genes among V. ursinus populations; however, this study is the first to identify MHC genes in a wombat and will advance immunological and disease studies of the species.