Julie M Old
Western Sydney University, Science, Faculty Member
- Associate Professor Julie Old joined Western Sydney University in 2006. She has expertise in comparative and developm... moreAssociate Professor Julie Old joined Western Sydney University in 2006. She has expertise in comparative and developmental immunology, native mammal biology, wildlife management, conservation and citizen science. She completed her PhD in 2002 in marsupial immunology and worked as a postdoctoral research fellow at Macquarie University prior to commencing her position at WSU. Julie’s research combines immunology, developmental biology, molecular biology, anatomy, microscopy, ecological techniques and citizen science to solve important issues in wildlife health and disease, especially marsupials. She established the School of Science and Health Small Native Mammal Teaching and Research Facility in 2009, and is the Chief Investigator of the Citizen Science project, WomSAT. Since 1998, her research has resulted in over 110 research publications in high impact journals including Science, PLoS One and DCI, and a regular reviewer of national and international journals and research grant applications. Julie is a Board member of The Wombat Foundation, and a member of the NSW National Parks and Wildlife Service Advisory Council, NSW NPWS Audit and Compliance Committee, NSW NPWS Regional Advisory Committee, Environmental Trust Invasive Species Technical Review Committee, and formerly a Director and the President of the Australian Wildlife Society (2018-2023). Julie has also been recognised internationally as a Senior Fellow by the Higher Education Academy for her excellence in teaching undergraduate and postgraduate students, and support of those new to teaching in Higher Education.edit
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Quolls are carnivorous marsupials in the family Dasyuridae with characteristic white spots. They are distributed throughout Australia and New Guinea, but uncommonly seen due to their mostly nocturnal solitary nature, and large home... more
Quolls are carnivorous marsupials in the family Dasyuridae with characteristic white spots. They are distributed throughout Australia and New Guinea, but uncommonly seen due to their mostly nocturnal solitary nature, and large home ranges. All Australian quolls are listed as ‘near threatened' or ‘endangered' at state, national and international levels, largely due to human-induced threats. Threats include introduced predators, habitat loss through clearing and modifications including changed fire regimes, disease, human persecution, vehicle collisions and accidental or targeted poisoning by humans and cane toads (Rhinella marina). Conservation efforts that have focussed on reducing introduced predators, and minimising the impact of cane toads, have aided some translocations, hence species recovery in some local areas of Australia has occurred. Where species conservation has required captive breeding for translocation, successful captive management has been crucial. We summar...
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There are three species of wombat, two hairy-nosed and the barenosed wombat (Vombatus ursinus). The southern hairy-nosed wombat (Lasiorhinus latifrons) is threatened (Woinarski & Burbidge, 2016) whilst the northern hairy-nosed wombat... more
There are three species of wombat, two hairy-nosed and the barenosed wombat (Vombatus ursinus). The southern hairy-nosed wombat (Lasiorhinus latifrons) is threatened (Woinarski & Burbidge, 2016) whilst the northern hairy-nosed wombat (Lasiorhinus krefftii) is critically endangered (Taggart, Martin, & Horsup, 2016). The bare-nosed wombat is regarded as Least Concern (Taggart, Martin, & Menkhorst, 2016). All wombats are large native herbivorous grazers, with many adaptations to Australia's low-productivity habitats (Evans et al., 2006; Johnson, 1998; Sharp & Trusler, 2015). The most obvious and wellknown adaptation is burrowing. Burrowing allows wombats to increase home range size, without needing to increase energy expenditure
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The pro-inflammatory cytokine, Interleukin-6 (IL-6), has not yet been fully characterised in the model macropod, Macropus eugenii, due to incomplete sequence information in publically available genome databases. Using a Rapid... more
The pro-inflammatory cytokine, Interleukin-6 (IL-6), has not yet been fully characterised in the model macropod, Macropus eugenii, due to incomplete sequence information in publically available genome databases. Using a Rapid Amplification of cDNA Ends strategy we have confirmed the expression and complete nucleotide sequence for this molecule in lymph node tissue and activated leukocytes. Structural conservation of the mature wallaby IL-6 molecule was high when compared with human IL-6, although there was only 34% amino acid sequence identity with the human IL-6 peptide, consistent with reports of the evolutionary divergence of this cytokine. We also report the discovery of MeIL-6Δ2, a splice variant missing exon 2, which directly translates to a truncated non-functional peptide, but which may also code for an alternative peptide that is translated downstream of the canonical IL-6 start site. This putative gene product is predicted to maintain some, if not all, of the functions of macropod IL-6 and is the first IL-6 isoform reported outside of eutherian mammals.
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There is currently limited information regarding the levels of infection and distribution of sarcoptic mange in the wombat population throughout Australia. We analyzed cases of sarcoptic mange in bare-nosed wombats reported into WomSAT, a... more
There is currently limited information regarding the levels of infection and distribution of sarcoptic mange in the wombat population throughout Australia. We analyzed cases of sarcoptic mange in bare-nosed wombats reported into WomSAT, a website and mobile phone application where citizen scientists can upload sightings of wombats, burrows, and sarcoptic mange status. We used Maxent software to predict locations and the environmental factors associated with sarcoptic mange occurrence in bare-nosed wombats. A total of 1379 sarcoptic mange-infected and 3043 non-sarcoptic mange-infected wombats were reported by 674 and 841 citizen scientists, respectively. Of all the wombats reported to WomSAT from 2015 to 2019, 31.2% were infected with sarcoptic mange. Sarcoptic mange in bare-nosed wombats was reported in 502 suburbs across four states. New South Wales had the highest number of sarcoptic mange cases reported to WomSAT. There was no statistically significant seasonal variation of sarcoptic mange levels in bare-nosed wombats. The model showed that Euclidean distance to urban areas was the highest contributing factor for sarcoptic mange occurrence. As distance to urban areas decreased, the suitability for sarcoptic mange increased. Annual precipitation was the next contributing factor in the model, with higher rainfall of 400-700 mm correlating to an increase in sarcoptic mange occurrence. As the data collected to date have provided the largest-scale contemporary distribution of sarcoptic mange in wombats, data should continue to be collected by citizen scientists as it is an easy and low-cost method of collecting data over large areas. We suggest targeting the identified hotspot areas and more site-specific studies for studying and mitigating sarcoptic mange in bare-nosed wombats.
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This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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Roads directly impact wildlife through vehicle collisions. In Australia only a few studies have examined the impact of environmental characteristics on wombat roadkill. We analysed wombat roadkills reported into WomSAT, a website and... more
Roads directly impact wildlife through vehicle collisions. In Australia only a few studies have examined the impact of environmental characteristics on wombat roadkill. We analysed wombat roadkills reported into WomSAT, a website and application where citizen scientists can upload sightings of wombats, to map wombat roadkill across their distribution. We used Maxent software to identify the main factors influencing wombat roadkill hotspots. A total of 2391 wombat deaths on roads were reported by 567 citizen scientists from 2015 to 2019. More wombat roadkill deaths occurred in winter, with most unaffected by sarcoptic mange. The average daily solar exposure, distance to populated areas, precipitation of warmest quarter (bio 18), temperature seasonality (bio 4), and precipitation seasonality (bio 15) were identified as the main factors influencing wombat roadkill. The most roadkills reported per kilometre (3.7/km) occurred on Old Bega Road and Steeple Flat Road, in the New South Wales...
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Wombats suffer from sarcoptic mange, a mite infection that ultimately leads to their death from secondary infections. In 2017, wildlife carers were granted legal approval to treat bare-nosed wombats (Vombatus ursinus) for sarcoptic mange... more
Wombats suffer from sarcoptic mange, a mite infection that ultimately leads to their death from secondary infections. In 2017, wildlife carers were granted legal approval to treat bare-nosed wombats (Vombatus ursinus) for sarcoptic mange in the field using 4 mL of topical Cydectin® per adult wombat. However, (limited) scientific field trials suggest approved protocols are inadequate which has been supported anecdotally by wildlife carers. Elucidating carer experience is key to holistically advancing understandings of sarcoptic mange treatment. We interviewed 18 wildlife carers regarding the use of Cydectin® to treat free-ranging adult wombats infected with sarcoptic mange which uncovered 43 detailed case studies for examination. Case studies revealed that wildlife carers have used 10-200-mL doses of topical Cydectin® to treat wombats to recovery. These results suggest there is no best-fit for treating wombats in the field, due to individual differences in observed levels of sarcoptic mange severity and differences in wombat behavior. Furthermore, wildlife carers suggested pour-on Cydectin® appeared non-toxic to wombats at rates as high as 200 mL per treatment. We recommend scientific trials should be undertaken to determine the impact and efficacy of the varying treatment regimens, including low and high doses of topical Cydectin® on bare-nosed wombats. This information is required for regulating authorities, and subsequently wildlife carers, and managers, to make fully informed decisions about wombat sarcoptic mange treatment.
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Background Roads have major ecological impacts on wildlife. Vehicle collisions most frequently impact large herbivores due to their larger home range compared to smaller animals, and higher population density compared to carnivores.... more
Background Roads have major ecological impacts on wildlife. Vehicle collisions most frequently impact large herbivores due to their larger home range compared to smaller animals, and higher population density compared to carnivores. Kangaroos (Macropus spp.) account for a large proportion of reported wildlife vehicle collisions that occur in N S W, Australia. We aimed to evaluate what influenced road mortality of eastern grey kangaroos (Macropus giganteus) in a temperate rural/suburban region. The location of roadkilled kangaroos found on or near two 1 km stretches of road in Richmond NSW was recorded throughout 2014 and 2015. Weather and moon phase data were recorded for the date of each roadkilled kangaroo. Transects were setup on both roads, and multiple road and landscape features, including the width of roadside, fence construction, habitat type, and distance from street lights measured at 50 m intervals. Data were analyzed to explore which landscape features and temporal facto...
Research Interests: Geography, Ecology, Marsupials, Habitat, Wildlife, and 4 moreRural Areas, Roads, Eastern Grey Kangaroo, and Roadkill
Populations of the common ringtail possum (Pseudocheirus peregrinus) in inland New South Wales have declined or disappeared. Habitat requirements and diet of these populations are poorly understood. Determining the diet of inland ringtail... more
Populations of the common ringtail possum (Pseudocheirus peregrinus) in inland New South Wales have declined or disappeared. Habitat requirements and diet of these populations are poorly understood. Determining the diet of inland ringtail possums is crucial to understanding the factors limiting their survival, and was the focus of this study. Spotlighting surveys were conducted to locate ringtail possums, and scat and vegetation samples were collected for microhistological analysis. Ringtail possums were most frequently observed in red stringybark followed by bundy box and black cypress pine trees, and this correlated with the most common dietary items consumed.
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Hearing is essential for communication, to locate prey and to avoid predators. We addressed the paucity of information regarding hearing in Australian native mammals by specifically assessing the hearing range and sensitivity of the... more
Hearing is essential for communication, to locate prey and to avoid predators. We addressed the paucity of information regarding hearing in Australian native mammals by specifically assessing the hearing range and sensitivity of the red-tailed phascogale (Phascogale calura), the kultarr (Antechinomys laniger) and the spinifex hopping-mouse (Notomys alexis). Auditory brainstem response (ABR) audiograms were used to estimate hearing thresholds within the range of 1–84 kHz, over a dynamic range of 0–80 dB sound pressure level (SPL). Phascogales had a hearing range of 1–40 kHz, kultarrs 1–35 kHz and hopping-mice 1–35 kHz, with a dynamic range of 17–59 dB SPL, 20–80 dB SPL and 30–73 dB SPL, respectively. Hearing for all species was most sensitive at 8 kHz. Age showed no influence on optimal hearing, but younger animals had more diverse optimal hearing frequencies. There was a relationship between males and their optimal hearing frequency, and greater interaural distances of individual ma...
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People's behaviour towards the conservation of Australian wildlife is important, because people's actions are the main causative factor associated with the demise and future recovery of wildlife conservation. We investigated the... more
People's behaviour towards the conservation of Australian wildlife is important, because people's actions are the main causative factor associated with the demise and future recovery of wildlife conservation. We investigated the type and prevalence of behaviour performed by NSW residents towards wildlife conservation, investigate participants’ willingness to perform conservation behaviours in the future, and identify the barriers preventing people from engaging in conservation action. New South Wales (NSW) residents (n=312) participated in an online questionnaire in a cross-sectional study. Overall, participants’ intention to engage, and rates of actual participation, in conservation behaviours was low. The latter was mostly due to lack of time, money, knowledge, and the impact of health and fitness levels. The knowledge gained from this study can be harnessed to inform future policy and management decisions, design interventions to change conservation behaviours, and provid...
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B-cells are key to humoral immunity, are found in multiple lymphoid organs, and have the unique ability to mediate the production of antigen-specific antibodies in the presence of pathogens. The marsupial immunoglobulin (Ig) heavy (H)... more
B-cells are key to humoral immunity, are found in multiple lymphoid organs, and have the unique ability to mediate the production of antigen-specific antibodies in the presence of pathogens. The marsupial immunoglobulin (Ig) heavy (H) chain locus encodes four constant region isotypes, IgA, IgG, IgM and IgE, but no IgD, and there are two light (L) chain isotypes, lambda (Igλ) and kappa (Igκ). To gain an understanding of the marsupial humoral immune system, B-cell transcriptomes generated by single-cell RNA sequencing from gray short-tailed opossum (Monodelphis domestica) splenocytes, and peripheral blood mononuclear cells were analysed. The cells used were from a single unimmunized animal and the majority of B-cells were transcribing IgM heavy chains. The ratio of Ig light chain use was roughly 2:1, Igλ:Igκ in this individual. This was not predicted due to Igκ being the more complex of the two L chain loci. The variable (V) gene segment pairs used in individual B-cells confirm greater diversity provided by the L chain V. This study is the first to report on using single cell analysis to investigate Ig repertoires in a marsupial and confirms a number of prior hypothesis, as well as revealing some surprises.
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Roads directly impact wildlife through vehicle collisions. In Australia only a few studies have examined the impact of environmental characteristics on wombat roadkill. We analysed wombat roadkills reported into WomSAT, a website and... more
Roads directly impact wildlife through vehicle collisions. In Australia only a few studies have examined the impact of environmental characteristics on wombat roadkill. We analysed wombat roadkills reported into WomSAT, a website and application where citizen scientists can upload sightings of wombats, to map wombat roadkill across their distribution. We used Maxent software to identify the main factors influencing wombat roadkill hotspots. A total of 2391 wombat deaths on roads were reported by 567 citizen scientists from 2015 to 2019. More wombat roadkill deaths occurred in winter, with most unaffected by sarcoptic mange. The average daily solar exposure, distance to populated areas, precipitation of warmest quarter (bio 18), temperature seasonality (bio 4), and precipitation seasonality (bio 15) were identified as the main factors influencing wombat roadkill. The most roadkills reported per kilometre (3.7/km) occurred on Old Bega Road and Steeple Flat Road, in the New South Wales southern tablelands. Reduced reporting corresponded with a reduced number of citizen scientists continuing to report sightings over time, reflecting the limitations of the data collected by citizen scientists. However, data should continue to be collected by citizen scientists as it is an easy and low-cost method of collecting data over large areas, and can provide information to managers to support conservation efforts directed at reducing roadkill. Our study emphasises the need to continue to engage and reward citizen scientists for their efforts.
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There are many limitations when using traditional laboratory species. Limits on variation, may result in limited outcomes, at both the species and individual level, due to different individuals/species having diverse physiological... more
There are many limitations when using traditional laboratory species. Limits on variation, may result in limited outcomes, at both the species and individual level, due to different individuals/species having diverse physiological processes, or differing molecular and genetic mechanisms. By using a variety of model species, we will be able to develop creative solutions to biological problems and identify differences of which we were not previously aware. The laboratory mouse has been a suitable model species for various mammalian studies, however most are bred specifically for laboratory research with limited variability due to selective breeding. Marsupial models offer unique research opportunities compared to eutherian models. We believe that there should be an expansion in marsupial model species, and the introduction of the red-tailed phascogale (Phascogale calura), a dasyurid marsupial, should be one of them. Phascogales are easily managed in captivity, and there are now multiple studies involving their development, reproduction, nutrition, behavior and immune system, which can serve as a baseline for future studies. The addition of the phascogale as a model species will improve future mammalian studies by introducing variability and offer alternate solutions to biological problems, particularly in the areas of genetics, nutrition, immunology, the neuro-endocrine system, and ageing, due to their semelparous reproductive strategy and hence, subsequent predictive physiology. In this review, we provide information based on existing research on red-tailed phascogales to support their inclusion as a model species.
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Mosquito-borne viruses are well recognized as a global public health burden amongst humans, but the effects on non-human vertebrates is rarely reported. Australia, houses a number of endemic mosquito-borne viruses, such as Ross River... more
Mosquito-borne viruses are well recognized as a global public health burden amongst humans, but the effects on non-human vertebrates is rarely reported. Australia, houses a number of endemic mosquito-borne viruses, such as Ross River virus, Barmah Forest virus, and Murray Valley encephalitis virus. In this review, we synthesize the current state of mosquito-borne viruses impacting non-human vertebrates in Australia, including diseases that could be introduced due to local mosquito distribution. Given the unique island biogeography of Australia and the endemism of vertebrate species (including macropods and monotremes), Australia is highly susceptible to foreign mosquito species becoming established, and mosquito-borne viruses becoming endemic alongside novel reservoirs. For each virus, we summarize the known geographic distribution, mosquito vectors, vertebrate hosts, clinical signs and treatments, and highlight the importance of including non-human vertebrates in the assessment of ...
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Ticks (Arachnida: Acari) are vectors for pathogens and the biggest threat to animal health. Many Australian ticks are associated with pathogens that impact humans, domestic animals and livestock. However, little is known about the... more
Ticks (Arachnida: Acari) are vectors for pathogens and the biggest threat to animal health. Many Australian ticks are associated with pathogens that impact humans, domestic animals and livestock. However, little is known about the presence or impact of tick-borne pathogens in native Australian wildlife. Wombats are particularly susceptible to the effects of the ectoparasite Sarcoptes scabiei which causes sarcoptic mange, the reason for which is unknown. Factors such as other ectoparasites and their associated pathogens may play a role. A critical understanding of the species of ectoparasites that parasitise wombats and their pathogens, and particularly ticks, is therefore warranted. This review describes the ectoparasites of wombats, pathogens known to be associated with those ectoparasites, and related literature gaps. Pathogens have been isolated in most tick species that typically feed on wombats; however, there are minimal molecular studies to determine the presence of pathogens in any other wombat ectoparasites. The development of next-generation sequencing (NGS) technologies allows us to explore entire microbial communities in ectoparasite samples, allowing fast and accurate identification of potential pathogens in many samples at once. These new techniques have highlighted the diversity and uniqueness of native ticks and their microbiomes, including pathogens of potential medical and veterinary importance. An increased understanding of all ectoparasites that parasitise wombats, and their associated pathogens, requires further investigation.
Research Interests: Microbiology, Parasitology, Medical Microbiology, Infectious Diseases, Host-parasite Coevolution, and 11 moreMedicine, Emerging Infectious Diseases, Ticks, Marsupial, Parasites, Wombats, Ectoparasites, Host Parasite Interaction, Ticks and Tick Borne Diseases, Veterinary Sciences, and Animal Parasites
Quolls are carnivorous marsupials in the family Dasyuridae with characteristic white spots. They are distributed throughout Australia and New Guinea, but uncommonly seen due to their mostly nocturnal solitary nature, and large home... more
Quolls are carnivorous marsupials in the family Dasyuridae with characteristic white spots. They are distributed throughout Australia and New Guinea, but uncommonly seen due to their mostly nocturnal solitary nature, and large home ranges. All Australian quolls are listed as ‘near threatened’ or ‘endangered’ at state, national and international levels, largely due to human-induced threats. Threats include introduced predators, habitat loss through clearing and modifications including changed fire regimes, disease, human persecution, vehicle collisions and accidental or targeted poisoning by humans and cane toads (Rhinella marina). Conservation efforts that have focussed on reducing introduced predators, and minimising the impact of cane toads, have aided some translocations, hence species recovery in some local areas of Australia has occurred. Where species conservation has required captive breeding for translocation, successful captive management has been crucial. We summarise rese...
Research Interests: Zoology, Biology, Ecology, Marsupials, Dasyurids, and 5 moreQuoll, Marsupialia, Dasyuridae, Tiger Quoll, and Spotted Tail Quoll
Abstract Roadkill is a worldwide issue that can cause local population extinctions. In Australia numerous species are affected, however the bare-nosed wombat (Vombatus ursinus) is particularly vulnerable due to its preference for... more
Abstract Roadkill is a worldwide issue that can cause local population extinctions. In Australia numerous species are affected, however the bare-nosed wombat (Vombatus ursinus) is particularly vulnerable due to its preference for disturbed habitats. Collisions with motor vehicles causes significant damage to the vehicle and sometimes the driver, as well as wounding or killing the wombat. Virtual fences are light and sound-based devices, originally developed in Austria that can be used to reduce roadkill through mitigation. They have had mixed results. In this study a virtual fence was installed along a 1.5 km stretch of road in semi-rural NSW, Australia, with the aim of reducing wombat roadkill through the use of this form of mitigation strategy. The number of roadkilled wombats was counted before and after the fence was installed in March 2020. Prior to the fence being installed 23 wombats were killed and after the fence was installed six wombats were killed. Along Old Bega Road, outside of the fenced area 64 wombats were killed pre-fence installation and 17 post fence installation. Bare-nosed wombats are semi-fossorial ecosystem engineers with an important role in the ecosystem and despite being listed as Least Concern are readily impacted by roadkill. Virtual fencing implemented in regions that have high wombat roadkill rates may aid in reducing road deaths and species conservation. However, we recommend that more research is required to assess virtual fencing, as a roadkill mitigation strategy, including an investigation into a larger number of species in a range of different habitats.
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Moxidectin (MOX) is a macrocyclic lactone approved worldwide for the treatment of both endo- and ecto-parasites in many mammalian species. The aim of this study was to assess the efficacy of MOX as a treatment against parasites in a range... more
Moxidectin (MOX) is a macrocyclic lactone approved worldwide for the treatment of both endo- and ecto-parasites in many mammalian species. The aim of this study was to assess the efficacy of MOX as a treatment against parasites in a range of mammalian species. An electronic literature search was performed for publications to the 1st September 2020. A total of 205 papers were retrieved and screened against all required criteria; hence, 35 were papers were reviewed in this study. The level of evidence and methodological quality was analysed, where a total of 13 publications were categorised as a 'randomised control trial', seven were categorised as a 'non-randomised control trial' and 15 as an 'experimental control trial'. The overall methodological quality of the publications was considered low, low to moderate, moderate, moderate to high and high in ten, four, twelve, five and a further four, respectively. We assessed the treatment and possible toxicity of MOX in 13 mammalian species, six investigations reported adverse effects to MOX in a small percentage of individuals. The authors reported observed reactions that were typically mild symptoms that did not require additional therapies, and/or resolved themselves. Further studies are needed to assess the efficacy of MOX treatment in a larger number of species, particularly in wildlife.
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Studies of gene diversity are used to investigate population dynamics, including immunological fitness. Aside from the selection of an appropriate gene target, an important factor that underpins these studies is the ability to recover... more
Studies of gene diversity are used to investigate population dynamics, including immunological fitness. Aside from the selection of an appropriate gene target, an important factor that underpins these studies is the ability to recover viable DNA samples from native animals that are protected, threatened or difficult to sample or locate such as the bare-nosed wombat (Vombatus ursinus). In this study, we used genomic DNA extracted from muscle tissue samples and also identified the optimal method to extract DNA from fresh wombat scat samples to enable further analyses to be performed using non-invasive techniques. The DNA was probed via the polymerase chain reaction using previously targeted marsupial Major Histocompatibility Complex (MHC) gene primers. These genes are highly variable and associated with binding and presentation of pathogens in the immune system. Twenty-three unique MHC Class II DAB V. ursinus gene sequences were translated to 21 unique predicted peptide sequences from...
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Sarcoptic mange is caused by the mite Sarcoptes scabiei and has recently been recognized as an emerging infectious disease of wildlife worldwide. The mite is one of the main causes of population decline in southern hairy-nosed... more
Sarcoptic mange is caused by the mite Sarcoptes scabiei and has recently been recognized as an emerging infectious disease of wildlife worldwide. The mite is one of the main causes of population decline in southern hairy-nosed (Lasiorhinus latifrons) and bare-nosed wombats (Vombatus ursinus). This review focuses on Sarcoptes scabiei infestations in wombats and provides insights into why the disease may be so prevalent in wombats. Current treatment practices and trials conducted in the field to reduce the incidence of sarcoptic mange in wombats are described and critically reviewed. Current and potential future avenues of research are discussed.
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Camera traps are frequently used in wildlife research and may be a useful tool for monitoring behavioural patterns. The suitability of camera traps to monitor behaviour depends on the size, locomotion, and behaviour of the species being... more
Camera traps are frequently used in wildlife research and may be a useful tool for monitoring behavioural patterns. The suitability of camera traps to monitor behaviour depends on the size, locomotion, and behaviour of the species being investigated. The suitability of cameras for documenting the behaviour of eastern grey kangaroos was assessed here by comparing activity patterns collected using cameras to published activity patterns for the species. The activity patterns calculated from camera trap data were largely consistent with data from previous studies, although nocturnal activity appeared to be under-represented. Observations of unusual fighting behaviour illustrates the potential for camera traps to enable capture of novel observations. Kangaroo behaviour appeared to be influenced by the presence of cameras; however, no kangaroos retreated from cameras. Data suggested that kangaroos became habituated to cameras after eight months. The findings of this study suggest that cam...
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Expressed coding sequences for interleukin-6 (IL-6) and interleukin-6 receptor α (IL-6R) were examined in five marsupial species. Full length expressed coding sequences for IL-6 and IL-6R were identified and characterized in the gray... more
Expressed coding sequences for interleukin-6 (IL-6) and interleukin-6 receptor α (IL-6R) were examined in five marsupial species. Full length expressed coding sequences for IL-6 and IL-6R were identified and characterized in the gray short-tailed opossum (Monodelphis domestica). For IL-6, ∼225 bp fragments of the mRNA sequence were identified in the red-tailed phascogale (Phascogale calura), kultarr (Antechinomys laniger), and stripe-faced dunnart (Sminthopsis macroura), while ∼563 bp fragments of mRNA encoding IL-6R were identified in the red-tailed phascogale, kultarr, stripe-face dunnart and fat-tailed dunnart (Sminthopsis crassicaudata). Relative expression levels of IL-6 and IL-6R were examined in the heart, muscle, lung, liver, spleen and kidney of adult red-tailed phascogales, and IL-6 gene expression was found to be significantly higher in the lung and spleen than the other tissues examined, while the expression of IL-6R was significantly higher in the liver, lung and spleen...
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Diversity in major histocompatibility complex (MHC) genes can be correlated with the level of immunological fitness of an individual or group of individuals. This study tested published primer sets designed to amplify fragments of the MHC... more
Diversity in major histocompatibility complex (MHC) genes can be correlated with the level of immunological fitness of an individual or group of individuals. This study tested published primer sets designed to amplify fragments of the MHC Class II DAB and DBB genes to amplify the equivalent gene fragments in red-tailed phascogales (Phascogale calura). Seventeen genomic DNA samples extracted from phascogale muscle tissue were used to amplify the initial DAB and DBB fragments; however, only DAB PCR proved successful. The fragments were 172 bp in length between the primers and had a high level of identity to other known marsupial MHC Class II DAB gene sequences (89–98%), including those of the koala (Phascolarctos cinereus), Tasmanian devil (Sarcophilus harrisii), common brushtail possum (Trichosurus vulpecula) and several wallaby species. Multiple sequence alignment revealed limited variability of MHC Class II genes between the individuals, but eight individual sequences in total. Gen...
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Integrating comparative immunology can improve human, animal, and ecosystem health
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A recent publication in Parasitology Research by (Old et al. Parasitol Res 120:1077-1090, 2021) raises the topical and often controversial issue of the treatment of wildlife by personnel with little or no formal scientific training (e.g.... more
A recent publication in Parasitology Research by (Old et al. Parasitol Res 120:1077-1090, 2021) raises the topical and often controversial issue of the treatment of wildlife by personnel with little or no formal scientific training (e.g. wildlife carers). In a valuable contribution to the subject, Old and colleagues document a wide range of topical (pour-on) application doses and frequencies of moxidectin (Cydectin®) administered in situ to bare-nosed wombats (Vombatus ursinus) by members of the wildlife carer/treater community in southeast Australia to treat sarcoptic mange disease. This treatment occurred under minor use permits issued by the Australian Pesticides and Veterinary Management Authority (APVMA). These permits do not require veterinary supervision, although carers are registered and are expected to comply with the guidelines of this permit. The prevalence and severity of sarcoptic mange in wildlife is influenced by a variety of factors including mite biology, environmental conditions, population density, animal behaviour and immune susceptibility (Browne et al. Bioscience, 2021). In bare-nosed wombats, combinations of these elements play a substantial role in making the treatment of an already difficult disease more complex. (Moroni et al. Parasit Vectors 13:471, 2020) comment that any pharmacological treatment of freeranging wildlife must consider these factors when assessing their feasibility and implications, especially in the context of emerging drug resistance and potential long-term ecological impacts. As individuals with significant interest in sarcoptic mange and representing a range of professional research and veterinary expertise, we see value in providing expert commentary on this issue.
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Roadkill is a worldwide issue that can cause local population extinctions. In Australia numerous species are affected, however the bare-nosed wombat (Vombatus ursinus) is particularly vulnerable due to its preference for disturbed... more
Roadkill is a worldwide issue that can cause local population extinctions. In Australia numerous species are affected, however the bare-nosed wombat (Vombatus ursinus) is particularly vulnerable due to its preference for disturbed habitats. Collisions with motor vehicles causes significant damage to the vehicle and sometimes the driver, as well as wounding or killing the wombat. Virtual fences are light and sound-based devices, originally developed in Austria that can be used to reduce roadkill through mitigation. They have had mixed results. In this study a virtual fence was installed along a 1.5 km stretch of road in semi-rural NSW, Australia, with the aim of reducing wombat roadkill through the use of this form of mitigation strategy. The number of roadkilled wombats was counted before and after the fence was installed in March 2020. Prior to the fence being installed 23 wombats were killed and after the fence was installed six wombats were killed. Along Old Bega Road, outside of the fenced area 64 wombats were killed pre-fence installation and 17 post fence installation. Bare-nosed wombats are semi-fossorial ecosystem engineers with an important role in the ecosystem and despite being listed as Least Concern are readily impacted by roadkill. Virtual fencing implemented in regions that have high wombat roadkill rates may aid in reducing road deaths and species conservation. However, we recommend that more research is required to assess virtual fencing, as a roadkill mitigation strategy, including an investigation into a larger number of species in a range of different habitats.
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Single-cell transcriptome analysis of the B-cell repertoire reveals the usage of immunoglobulins in the gray short-tailed opossum (Monodelphis domestica)
Moxidectin (MOX) is a macrocyclic lactone approved worldwide for the treatment of both endo-and ecto-parasites in many mammalian species. The aim of this study was to assess the efficacy of MOX as a treatment against parasites in a range... more
Moxidectin (MOX) is a macrocyclic lactone approved worldwide for the treatment of both endo-and ecto-parasites in many mammalian species. The aim of this study was to assess the efficacy of MOX as a treatment against parasites in a range of mammalian species. An electronic literature search was performed for publications to the 1 st September 2020. A total of 205 papers were retrieved and screened against all required criteria; hence, 35 were papers were reviewed in this study. The level of evidence and methodological quality was analysed, where a total of 13 publications were categorised as a 'randomised control trial', seven were categorised as a 'non-randomised control trial' and 15 as an 'experimental control trial'. The overall method-ological quality of the publications was considered low, low to moderate, moderate, moderate to high and high in ten, four, twelve, five and a further four, respectively. We assessed the treatment and possible toxicity of MOX in 13 mammalian species, six investigations reported adverse effects to MOX in a small percentage of individuals. The authors reported observed reactions that were typically mild symptoms that did not require additional therapies, and/or resolved themselves. Further studies are needed to assess the efficacy of MOX treatment in a larger number of species, particularly in wildlife.
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All mammals are characterized by the ability of females to produce milk. Marsupial (metatherian) and monotreme (prototherian) young are born in a highly altricial state and rely on their mother’s milk for the first part of their life.... more
All mammals are characterized by the ability of females to produce milk. Marsupial (metatherian) and monotreme (prototherian) young are born in a highly altricial state and rely on their mother’s milk for the first part of their life. Here we review the role and importance of milk in marsupial and monotreme development. Milk is the primary source of sustenance for young marsupials and monotremes and its composition varies at different stages of development. We applied nutritional geometry techniques to a limited number of species with values available to analyze changes in macronutrient composition of milk at different stages. Macronutrient energy composition of marsupial milk varies between species and changes concentration during the course of lactation. As well as nourishment, marsupial and monotreme milk supplies growth and immune factors. Neonates are unable to mount a specific immune response shortly after birth and therefore rely on immunoglobulins, immunological cells and ot...
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Much of the literature on wombat diet is concerned only with the broad categories of consumed matter, and there is little about their specific nutritional requirements despite diet playing a major role in health. This study gathered... more
Much of the literature on wombat diet is concerned only with the broad categories of consumed matter, and there is little about their specific nutritional requirements despite diet playing a major role in health. This study gathered information by surveying zoological parks and wildlife carers about current feeding regime, diet and the prevalence of nutritional or dental disorders of wombats held in captivity, with the aim of improving the diets of captive wombats, thus their health and welfare outcomes. Data on the composition, variation and basis of diets for 31 bare-nosed or common wombats (Vombatus ursinus) and 19 southern hairy-nosed wombats (Lasiorhinus latifrons) were gathered from 13 facilities. The nutritional values of diets fed to wombats in captivity were calculated and compared with known dietary requirements for bare-nosed wombats. Results demonstrate that the current diet of wombats held in captivity often consists of a high portion of fruits, vegetables and livestock...
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Sarcoptic mange is a threat to bare-nosed wombats (Vombatus ursinus) in Australia and a major factor contributing to the decline in populations of this species. It is caused by a mite, Sarcoptes scabiei, and if untreated can lead to... more
Sarcoptic mange is a threat to bare-nosed wombats (Vombatus ursinus) in Australia and a major factor contributing to the decline in populations of this species. It is caused by a mite, Sarcoptes scabiei, and if untreated can lead to severe irritation, blindness, starvation and eventual death. Modes of transmission likely occur through direct contact between wombats and indirectly from wombat burrows. Our study aimed to estimate and compare the numbers of bare-nosed wombats at three study sites, and to determine how rainfall, temperature and humidity influenced the incidence of sarcoptic mange in the populations. The abundances of macropods and rabbits were also compared with wombat abundance and sarcoptic mange prevalence at the three study sites. Across the study sites, 1655 bare-nosed wombats were observed. Sarcoptic mange prevalence was between 7.0% and 40.7%, depending on site, season and year. Sarcoptic mange prevalence correlated with yearly rainfall, with a higher incidence o...
Research Interests: Parasitology, Zoology, Infectious disease epidemiology, Ecology, Mammalogy, and 14 moreMarsupials, Parasite Ecology, Wildlife Diseases, Mammals, Veterinary Parasitology, Parasites, Host-parasite interactions, Wombats, Vombatiformes, Mites, Animal Parasites, Sarcoptic Mange, Public Health Significance of Zoonotic Parasites, and Vombatidae
In this review we investigated the diet and nutrition of wombats and highlight areas for future research. The low energy requirements of wombats, conservative foraging behaviours and burrowing lifestyle allow them to subsist on... more
In this review we investigated the diet and nutrition of wombats and highlight areas for future research. The low energy requirements of wombats, conservative foraging behaviours and burrowing lifestyle allow them to subsist on low-quality food. Furthermore, their digestive anatomy and physiology is well adapted for digesting the resources available in their habitats. All species feed predominantly on native grasses; however, the nutritional requirements of juvenile and aged wombats are largely unknown. Changes to food availability and grass species in wombat habitats may alter their diet and nutritional intake. Also, despite evidence suggesting that wombats can satisfy their water requirements via the food they eat, the minimum threshold of water content at which wombats will cease feeding is unknown. Other areas for future research include investigating factors that affect digesta retention times (such as age or lactation), and the development of a method to determine age, because...
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The authors have withdrawn this preprint due to erroneous posting.
People's attitudes towards the conservation of Australian wildlife is of particular importance as the types of attitudes people hold can have a significant impact on conservation solutions. We investigated attitudes held towards... more
People's attitudes towards the conservation of Australian wildlife is of particular importance as the types of attitudes people hold can have a significant impact on conservation solutions. We investigated attitudes held towards Australian wildlife and conservation solutions. A sample of 312 residents participated in an online questionnaire. An ‘ecoscientistic’ attitude was the most commonly held attitude, highlighting that wildlife are appreciated for the role they play within our ecosystem. There was a significant association between age and attitude towards Koala Phascolarctos cinereus and Crownof-thorns starfish Acanthaster planci conservation and a significant association between socio-economic status and attitude towards Brush-turkey Alectura lathami conservation. Most participants agreed that action should be taken towards wildlife conservation in the future. Conservation managers and other key stakeholders need to capitalise on this information to increase public support...
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Ectoparasites are a leading cause of arthropod-borne disease in animals, and humans. Defined as arthropods which spend an entire portion of their life cycle on the host, ectoparasites include the ticks and mites (Acarina), and the lice... more
Ectoparasites are a leading cause of arthropod-borne disease in animals, and humans. Defined as arthropods which spend an entire portion of their life cycle on the host, ectoparasites include the ticks and mites (Acarina), and the lice and fleas of the insect family. Their role in human disease transmission has been well documented, as has their importance in agricultural and domestic animals. Little however has been done to comprehensively examine the role these organisms may play in disease transmission and their impact upon native Australian fauna. It is important to consider the effects of such disease agents on the survival of both captive and wild native animal populations, particularly as exposure to a novel pathogen may remove endangered animals that are a vital pool of genetic diversity.
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Wombats are large marsupial grazers native to Australia. They are ‘ecosystem engineers’ due to their burrowing, impacting rates of soil turnover, assisting with nutrient cycling and water infiltration, and creating habitat for other... more
Wombats are large marsupial grazers native to Australia. They are ‘ecosystem engineers’ due to their burrowing, impacting rates of soil turnover, assisting with nutrient cycling and water infiltration, and creating habitat for other species. The aim of this review is to provide an overview of available information on the habitat and distribution, abundance and threats to bare-nosed wombats (Vombatus ursinus), and to provide a critical review of the current gaps in our knowledge to aid future management of the species. Bare-nosed wombats were previously widespread. Since European settlement their range has reduced. While habitat loss and contraction impact population numbers, bare-nosed wombat populations are also threatened by roadkill, introduced species, legal and illegal human persecution, and disease. Further research is required to understand the historic range of bare-nosed wombats, as well as current population numbers, so that we can determine the threats that most significa...