- Prof.Dr.2 da Faculdade de Ciencias Farmacêuticas de Ribeirao Preto-USPDedit
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Direct enantioselective separation on chiral stationary phases and indirect separation based on the formation of diastereomeric derivatives were developed and compared for the HPLC analysis of R(+) and S(-)-metoprolol in human plasma.... more
Direct enantioselective separation on chiral stationary phases and indirect separation based on the formation of diastereomeric derivatives were developed and compared for the HPLC analysis of R(+) and S(-)-metoprolol in human plasma. Plasma samples prepared using solid-phase extraction columns or liquid-liquid extraction were directly analyzed on a Chiralpack AD or on a Chiralcel OD-H columns, respectively. S-(-)-menthyl choroformate was also used to yield diastereomeric derivatives resolved on a RP-8 column. The methods were employed to determine plasma concentrations of metoprolol enantiomers in a pharmacokinetic study of single dose administration of racemic metoprolol to a healthy Caucasian volunteer phenotyped as extensive metabolizer of debrisoquine. The correlation coefficients among enantioselective metoprolol plasma concentrations (5-223 ng/ml) obtained by the three methods were equal or higher than 0.99. The direct method that employed the chiral column Chiralpak AD may be considered the most sensitive, although the three methods demonstrated interchangeable use in the pharmacokinetic investigation.
Research Interests: Chemistry, Chromatography, Kinetics, Pharmacokinetics, Medicine, and 14 moreSample Preparation, Humans, Liquid Liquid Extraction, Solid Phase Extraction, High Performance Liquid Chromatography, High Pressure Liquid Chromatography, Enantioselective Synthesis, Reproducibility of Results, Correlation coefficient, Sensitivity and Specificity, Direct Method, Enantiomer, Metoprolol, and stereoisomerism
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Research Interests: Chemistry, Chromatography, Brazil, Drug metabolism, Medicine, and 15 moreHumans, Hypertension, Calibration, Female, Male, High Pressure Liquid Chromatography, Aged, Middle Aged, Adult, European Continental Ancestry Group, Molecular Conformation, Hydroxylation, Metabolite, Essential Hypertension, and Antihypertensive agents
A new high-performance liquid chromatography method is presented for the determination of 10 frequently prescribed tricyclic and nontricyclic antidepressants: imipramine, amitriptyline, clomipramine, fluoxetine, sertraline, paroxetine,... more
A new high-performance liquid chromatography method is presented for the determination of 10 frequently prescribed tricyclic and nontricyclic antidepressants: imipramine, amitriptyline, clomipramine, fluoxetine, sertraline, paroxetine, citalopram, mirtazapine, moclobemide and duloxetine. The simple and accurate sample preparation step, consisted of liquid:liquid extraction with recoveries ranging between 72% and 86%, except for moclobemide (59%). Separation was obtained using a reverse phase Select B column under isocratic conditions with UV detection (230 nm). The mobile phase consisted of 35% of a mixture of acetonitrile/methanol (92:8, v/v) and 65% of 0.25 mol L(-1) sodium acetate buffer, pH 4.5. The standard curves were linear over a working range of 2.5-1000 ng mL(-1) for moclobemide, 5-2000 ng mL(-1) for citalopram, duloxetine, fluoxetine, 10-2000 ng mL(-1) for sertraline, imipramine, paroxetine, mirtazapine and clomipramine. The intra-assay and inter-assay precision and accuracy were studied at three concentrations (50, 200, and 500 ng mL(-1)). The intra-assay coefficients of variation (CVs) for all compounds were less than 8.8%, and all inter-CVs were less than 10%. Limits of quantification were 2.5 ng mL(-1) for moclobemide, 5 ng mL(-1) for citalopram, duloxetine and amitriptyline, and 10 ng mL(-1) for mirtazapine, paroxetine, imipramine, fluoxetine, sertraline, and clomipramine. No interference of the drugs normally associated with antidepressants was observed. The method has been successfully applied to the analysis of real samples, for the drug monitoring of ten frequently prescribed tricyclic and non-tricyclic antidepressant drugs.
Research Interests: Chemistry, Analytical Chemistry, Chromatography, Humans, Liquid Liquid Extraction, and 15 moreCalibration, Female, Male, Coefficient of Variation, High Performance Liquid Chromatography, High Pressure Liquid Chromatography, Aged, Adult, Citalopram, Amitriptyline, Imipramine, clomipramine, HPLC Chromatography, Antidepressive agents, and DOXEPIN
Background: Dyslipidemias are a major risk factor for the development of cardiovascular diseases, proven through large-scale observational studies. Objective: To analyze the costs of taking atorvastatin, simvastatin, bezafibrate or... more
Background: Dyslipidemias are a major risk factor for the development of cardiovascular diseases, proven through large-scale observational studies. Objective: To analyze the costs of taking atorvastatin, simvastatin, bezafibrate or ciprofibrate for individuals covered by the Exceptional Medicines Program run by the Ministry of Health at the Hospital das Clinicas in Ribeirao Preto, Sao Paulo State, Brazil, in 2007. Methods: This is an observational, descriptive crosssection study with a sample consisting of 332 (31.11%) persons selected randomly from among 1067 male and female patients referred by the Unified National Health System (SUS) and private clinics, who were interviewed and their medical records were examined. Results: Of the 312 patients interviewed, 157 (51%) were male, between 15 and 63 (62.0±12.23) years old; 227 (73.22%) patients were taking statins and 54 (17.42%) were taking fibrates; 31(10%) formed the control group. The atorvastatin group presented the highest treatment cost (R$ 994.69 patient/year), while the simvastatin group (R$337.61 patient/year) spent more on laboratory examinations and supplementary tests. In the fibrates group, the drugs category resulted in higher expenditures for both groups. Patients in the ciprofibrate group needed more laboratory examinations and supplementary tests than the bezafibrate group. Conclusions: Although treatment with atorvastatin was the most expensive, these patients presented fewer cardiovascular events and procedures, in addition to lower outlays on laboratory examinations and supplementary tests.
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Background: A study published in 1967 analyzing annual mortality rates for hypertensive heart diseases adjusted by sex and age of 100,000 inhabitants from 1962 to 1964, listed three cities with the highest annual mortality rates due to... more
Background: A study published in 1967 analyzing annual mortality rates for hypertensive heart diseases adjusted by sex and age of 100,000 inhabitants from 1962 to 1964, listed three cities with the highest annual mortality rates due to hypertensive heart diseases (HHD): Ribeirao Preto (34.2) and Sao Paulo (31.7) in Brazil, and Cali (31.6) in Colombia. Objective: To investigate possible causes of death among hypertensive patients at a public health unit in Ribeirao Preto, SP. Methods: The casuistics consisted of a sample of hypertensive patients selected in compliance with the JNC VII (2003) from among the 1601 patients seen in 1999 and under follow-up monitoring at the Cardiology and Hypertension Out-Patient Clinic (CSE-FMRPUSP). The study included male and female adults over 20 years old, with pregnant women excluded. There were 155 deaths (9.68%) in the sample: 75 (48.1%) female and 80 (51.3%) male. The data were collected at the Instituto Medico Legal, Sistema de Coleta e Analise de Estatisticas Vitais in Ribeirao Preto and the Servico de Verificacao de Obitos do Interior. Results: The most frequent causes of death were: acute myocardial infarction (12.9%); non-specified septicemia (11.6%); pneumonia (8.4%); cancer (5.8%); acute pulmonary edema (5.8%); congestive heart failure (4.5%); stroke (4.5%); cardiogenic shock (5.8%); respiratory failure (3.2%); flutter or ventricular fibrillation (2.0%). Conclusion: The main causa mortis for a significant percentage of patients treated in a public specialized
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Research Interests: Chemistry, Analytical Chemistry, Organic Chemistry, Medicine, Chirality, and 15 moreHumans, Chronic Disease, Female, High Performance Liquid Chromatography, Heart Disease, Aged, Middle Aged, Cardiac Arrhythmias, Adult, Biotransformation, Chagas Cardiomyopathy, Hydroxylation, Area Under Curve, Enantiomer, and Cmax
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Research Interests: Chemistry, Analytical Chemistry, Organic Chemistry, Chromatography, Kinetics, and 14 moreMedicine, Chirality, Humans, Circular Dichroism, Urine, High Performance Liquid Chromatography, High Pressure Liquid Chromatography, Aged, Middle Aged, Adult, Biotransformation, Enantiomer, Metoprolol, and stereoisomerism
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Research Interests: Polymorphism, Anthropometry, Brazil, Medicine, Humans, and 15 morePharmacology and Clinical pharmacy, Female, Male, Urine, Phenotype, High Pressure Liquid Chromatography, Aged, Urinalysis, Middle Aged, Genetic Polymorphism, European Continental Ancestry Group, Oxidation-Reduction, Heart Diseases, Metoprolol, and Pharmacology and pharmaceutical sciences
Research Interests: Chemistry, Analytical Chemistry, Chromatography, Medicine, Humans, and 15 moreLiquid Liquid Extraction, Coefficient of Variation, High Performance Liquid Chromatography, High Pressure Liquid Chromatography, Particle Size, Liquid Chromatography, Reproducibility of Results, Flow Rate, Sensitivity and Specificity, Linear System and Response, Biochemistry and cell biology, Enantiomer, Mianserin, Pharmacology and pharmaceutical sciences, and stereoisomerism
Research Interests: Chemistry, Analytical Chemistry, Chromatography, Medicine, Amylose, and 15 moreQuantitative analysis, Humans, Liquid Liquid Extraction, Pharmaceutical, Calibration, Plasma, Coefficient of Variation, High Pressure Liquid Chromatography, Liquid Chromatography, Reproducibility of Results, Quantitative Analysis, Metabolite, Enantiomer, Pharmacology and pharmaceutical sciences, and HPLC Chromatography
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Research Interests: Clinical Trials, Medicine, Humans, Internal Medicine, Hypertension, and 15 moreBlood Pressure, Diuretics, Female, Male, Microalbuminuria, Aged, Adult, Left ventricular hypertrophy, Cardiovascular Diseases, Diuretic, Doenças Cardiovasculares, Amiloride, Diuréticos, Antihypertensive agents, and Drug combinations
Research Interests: Kinetics, Pharmacokinetics, Medicine, Humans, Female, and 12 moreBlood sampling, Chagas disease, High Performance Liquid Chromatography, Middle Aged, Cardiac Arrhythmias, Heart Ventricles, Metabolite, Ventricular Arrhythmia, Enantiomer, Pharmacology and pharmaceutical sciences, stereoisomerism, and Cmax
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Research Interests: Endocrinology, Obesity, Adolescent, Medicine, Humans, and 15 moreInternal Medicine, Metformin, Cholesterol, Male, Placebos, Medical Physiology, Middle Aged, Adult, Analysis of Variance, FLUOXETINE, Oral Hypoglycemic Agents, Sibutramine, Arquivos brasileiros, Combined Modality Therapy, and Multicenter Studies as Topic
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Research Interests: Chemistry, Chromatography, Medicine, Amylose, Humans, and 11 moreLiquid Liquid Extraction, Urine, High Performance Liquid Chromatography, High Pressure Liquid Chromatography, Reproducibility of Results, Sensitivity and Specificity, Metabolite, Enantiomer, Relative Standard Deviation, protein precipitation, and stereoisomerism
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Two methods were developed for the determination of mexiletine enantiomers in plasma samples suitable for studies on the stereoselective disposition of this drug. Both methods used fluorescence detection to improve sensitivity and... more
Two methods were developed for the determination of mexiletine enantiomers in plasma samples suitable for studies on the stereoselective disposition of this drug. Both methods used fluorescence detection to improve sensitivity and selectivity. The direct enantioselective separation was based on the chiral resolution of mexiletine-2-naphthamide derivatives on a Chiralcel OJ column. The calibration curves were linear over the concentration range
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Research Interests: Chemistry, Analytical Chemistry, Organic Chemistry, Chromatography, Medicine, and 12 moreChirality, Humans, Circular Dichroism, Female, High Performance Liquid Chromatography, High Pressure Liquid Chromatography, Middle Aged, Derivatization, Hydroxylation, Enantiomer, stereoisomerism, and calibration curve
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Este trabalho tem por objetivo descrever as atividades da Associacao Ribeirao-pretana de Ensino, Pesquisa e Assistencia ao Hipertenso (Arepah) – entidade filantropica sem fins lucrativos, referentes ao periodo de outubro de 1996 a marco... more
Este trabalho tem por objetivo descrever as atividades da Associacao Ribeirao-pretana de Ensino, Pesquisa e Assistencia ao Hipertenso (Arepah) – entidade filantropica sem fins lucrativos, referentes ao periodo de outubro de 1996 a marco de 2010. As atividades de ensino foram constituidas por 84 palestras dirigidas a populacao, de diferentes temas de utilidade pratica, com a participacao de 2.312 pessoas, variando de 20 a 216 (media de 63,4) participantes por sessao. Foram distribuidas gratuitamente seis edicoes de um jornal educativo a 1.259 individuos cadastrados. As atividades de pesquisa resultaram em oito dissertacoes de mestrado, duas teses de doutorado, um trabalho de Livre Docencia, 15 trabalhos publicados em revistas internacionais, sete em revistas nacionais e 66 trabalhos apresentados em congressos. Em relacao as atividades assistenciais, foram organizadas 24 campanhas de utilidade publica (dez Dias de Prevencao e Combate a Hipertensao Arterial, dez Semanas do Coracao e quatro Dias de Controle do Colesterol), sendo que 1.785 pacientes estiveram em seguimento clinico num ambulatorio de referencia de Cardiologia e Hipertensao Arterial (CSE-FMRP-USP). Acredita-se que associacoes como esta possam constituir-se, a medio e longo prazo, numa importante estrategia para prevencao e reducao da morbimortalidade por doencas cardiovasculares, independente das acoes governamentais nesta area de saude publica.
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The Brazilian Society of Hypertension (BSH) yearly conducts the National Campaign to promote and encourage the cardiovascular health of the population. Objective: To characterize the profile of the population assisted in the “ Less... more
The Brazilian Society of Hypertension (BSH) yearly conducts the National Campaign to promote and encourage the cardiovascular health of the population. Objective: To characterize the profile of the population assisted in the “ Less Pressure Campaign ” in 2018. Methods: This is a descriptive cross-sectional study based on the campaign MMM with the general public of the São Paulo city Brazil in May. The campaign comprised multi-professional interventions made by five workshops: 1)blood pressure(BP)measurement with automatic devices; 2)exercise guidelines; 3)anthropometry, i.e., measurement of weight, height, and abdominal circumference (AC) and calculation of body mass index (BMI); 4)nutritional guidance; and 5)psychological orientation. Data analysis was in accordance with the VII BSH Guidelines, which considered values ≥140mmHg for systolic BP (SBP) and ≥90 mmHg for diastolic BP (DBP) as altered. Results: A total of 1184 individuals were assisted; of these, 49% (580) were men and 51%(604) were women. The mean age was 53.5±15 years, the total participants, 51% (627) declared they were normotensive, 32% (380) stated they were hypertensive and 15,3% (182) do not know if you have hypertension. The BP of 31% (380) of the individuals was altered compared with normotensive (627) as follows: SBP146.9±14mmHg vs SBP 115.8±12; DBP 90.9±10mmHg vs 76.1±8, and heart rate 78.9±14 vs of 79.2±12, respectively between hypertensive, normotensive individuals. The anthropometric data of the participants with altered BP compared with normotensive population were the following: weight was statistically significant (p=0,0407) 77±15kg vs 71.9±18Kg; BMI was statistically significant (p=0,0007) 27.5±14Kg/m2 vs 21.9±22Kg/m2; and AC was statistically significant (p=0,0000) 98.5±11 vs 96.4±11cm respectively between hypertensive and normotensive individuals. When analyzing the association between sex and hypertension, it was identified that 48.9% (186) hypertensive were female and 51% (194) were male, when the association was assessed no statistical difference was found (p=0.0658) between sex and hypertension. Conclusions: The study points to the need for investment in educational and awareness actions to promote better control of the hypertensive population