Fahrul Ilham

Onset and duration of estrus are the early indicator of the success of prostaglandin F2α on the implementation of the estrus synchronization. The purpose of this study is to determine the effect of prostaglandin F2α injected in the vulva submucosal to the onset and etrus duration of kacang goats. This research has been conducted in the District of Bone Pantai, Bolango Bone Regency, Gorontalo, Indonesia. Kacang goats were used in research are 12 and grouped by doses/tail, there are 0.25 ml, 0.50 ml, 0.75 ml, and 1.00 ml. Kacang goats housed in a single enclosure and fed with local natural feed that is often consumed; natural forage and bran. After the adaptation process of feed and maintenance management for 2 weeks, kacang goats injected with prostaglandin F2α 1 ml/tail in the vulva submucosal by 2 times of injection. Onset and estrus duration was monitored every hour since it was first injected. Descriptive analysis result obtained from the mean of onset of estrus in kacang goats were injected with 0.25 ml dosis is 39.5 hours, 0.50 ml dosis is 62 hours, 0.75 ml is 50.5 hours, and 1.00 mL was 38.5 hours. Estrus duration were injected at 0.25 ml dosis is 28 hours, 0.50 ml dosis is 30.5 hours, 0.75 ml is 28.5 hours, and 1.00 mL is 29.5 hours. Based on the Analysis of Variance (ANOVA), the number of doses doesn't give significant effects statistically (P>0.05) on the onset and estrus duration of kacang goats were injected PGF2α in the vulva submucosal. Keyword: dosis of prostaglandin F2α, estrus synchronization ABSTRAK Onset dan lama estrus merupakan indikator awal keberhasilan prostaglandin F2α pada pelaksanaan sinkronisasi birahi. Tujuan penelitian ini adalah untuk mengetahui pengaruh prostaglandin F2α yang diinjeksi pada submukosa vulva terhadap onset dan lama estrus kambing kacang. Penelitian ini telah dilaksanakan di Kecamatan Bone Pantai, Kabupaten Bone Bolango, Gorontalo. Kambing kacang yang digunakan sebanyak 12 ekor yang dikelompokkan berdasarkan dosis pemberian/ekor yaitu 0.25 ml, 0.50 ml, 0.75 ml, dan 1.00 ml. Ternak ditempatkan dalam satu kandang kelompok dan diberi pakan lokal alami yang sering dikonsumsi yaitu hijauan dan dedak. Setelah proses adaptasi pakan dan manajemen pemeliharaan selama 2 minggu, kambing kacang dinjeksi dengan prostaglandin F2α 1 ml/ekor pada submukosa vulva dengan 2 kali penyuntikan. Onset dan lama estrus diamati setiap jam sejak pertama kali diinjeksi. Hasil analisis deskriptif diperoleh rerata onset estrus pada kambing kacang yang diinjeksi pada dosis 0.25 ml adalah 39.5 jam, dosis 0.50 ml adalah 62 jam, 0.75 ml adalah 50.5 jam, dan 1.00 ml adalah 38.5 jam. Lama estrus yang yang diinjeksi pada dosis 0.25 ml adalah 28 jam, dosis 0.50 ml adalah 30.5 jam, 0.75 ml adalah 28.5 jam, dan 1.00 ml adalah 29.5 jam. Berdasarkan hasil analisis of varian (Anova) jumlah dosis tidak

Diversity Gen Growth Hormone (Gh) of Kacang Goat In Kota Gorontalo and Regency Of Bone Bolango (Province Of Gorontalo)

Fahrul Ilham 1, Safriyanto Dako 1, Agus Bahar Rachman 1, Muhammad Ihsan Andi Dagong2, and Lellah Rahim2

1 Department of Animal Science, Faculty of Agriculture, State University of Gorontalo, Gorontalo
2 Department of Animal Science, Faculty of Animal Science, University of Hasanuddin, Makassar

ABSTRACT
Growth Hormone (GH) is a hormone produced by cells in the anterior lobe of the pituitary somatrotop and formation process under the control of GH gene. One important function of this hormone is to help the process of tissue formation and metabolism of fat to meat forming. The
purpose of this study was to determine the genetic diversity of genes GH Kacang goat in subpopulations of Kota Gorontalo and Regency of Bone Bolango. Blood samples were used for DNA extraction process in Centre of Biotechnology Laboratory University of Hasanuddin is 41 samples of
Kacang goats with 21 samples from Kota Gorontalo city and 20 samples from Regency of Bone Bolango. Genomic DNA was extracted using a kit DNA extraction Genjet Genomic DNA Extraction (Thermo Scientific) following standard protocol phenol-chloroform, amplified by the technique of Polymerase Chain Reaction (PCR), and genotyping was done by Polymerase Chain ReactionRestriction Fragment Length Polymorphism (PCR-RFLP) using the restriction enzyme Hae III. Data
were analyzed descriptively by calculating the frequency of genotype, allele frequency, and degree of heterozygosity. The results showed GH genotype frequencies for the genotypes AA and AB were 2.45 and 97.5% respectively and the frequency of alleles A and B were 51.2 and 48.7% per cent respectively and the observed heterozygosity (Ho) and expected heterozygosity (He) were 0.97 and 0.50 respectively. Based on the sub-population genotype frequencies obtained GH gene of Kacang goat from Kota Gorontalo is 95.25 % for AA and 4.76% for AB, the frequency of allele A and B was 52.3% 47.6%, observated heterozygosity (Ho) 0.95 and expected heterozygosity (He ) 0.51. GH gen genotype frequencies in Kacang goat from Regency of Bone Bolango is AB 100%, the frequency of allele A and allele B 0.5 0.5, observation heterozygosity (Ho) of 1.00 and expectation heterozygosity (He) 0.51. Based on the results concluded GH gene Kacang goat from Kota Gorontalo and Regency of
Bone Bolango is polymorphic so that it can be used as the basis for the implementation of the selection.

Key Words: Genetic Diversity, Growth Hormone, Kacang Goat

Kacang goat is a small ruminant native from Indonesia and spread widely in several Southeast Asian countries, and has become part of the world's biodiversity. Growth Hormone (GH) in goat is a protein secreted from anterior hipophyse by somatrotop cells and its formation process under the control of GH gene. Its main function is to stimulate the growth of bone, muscle and fat metabolism. The purpose of this study is to identify the GH gene polymorphism in Kacang goat with Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) methods. A total of 168 blood samples from Kacang goat in two regions of Indonesia (Gorontalo and South Sulawesi province) were extracted by using DNA extraction kit to collect whole DNA genome. Amplification and genotyping of GH gene was performed using PCR-RFLP using the HaeIII restriction enzyme. The results obtained two kinds of genotypes, with the AA genotype frequencies (0.095) and AB (0.904). The frequency of allele A (0.547) and B (0.452) indicate a polymorphism in the GH A781G locus in Kacang goat. The observed (Ho) and expected (He) heterozygosity value were respectively 0.0904 and 0.496. GH alleles distribution in Kacang goat populations were not in Hardy-Weinberg equilibrium. Based on statistical analysis, there is no difference between body size of genotype AA and AB. The results of this study can be used as initial information in formulating a strategy for Kacang goat breeding by utilizing Marker Assisted Selection (MAS).