Compreender a regulação gênica é indispensável para o estudo de como a informação genética flui do DNA ao fenótipo através da síntese proteica. A fim de diminuir a abstração do tema, o material didático AmEG é uma alternativa metodológica... more
Compreender a regulação gênica é indispensável para o estudo de como a informação genética flui do DNA ao fenótipo através da síntese proteica. A fim de diminuir a abstração do tema, o material didático AmEG é uma alternativa metodológica para o processo de ensino-aprendizagem, que pode ser usado por professores do ensino superior.
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O GEA (Genes e Ambientes) é um jogo que mostra, de forma lúdica e visual, o efeito da seleção natural em uma ou mais populações de um mamífero hipotético, por meio de simulações da interação entre genótipos, fenótipos e o meio ambiente.
Neste trabalho didático, é apresentada uma alternativa para o ensino das alterações cromossômicas numéricas, as aneuploidias. A atividade é proposta na forma de um jogo cujas cartas, contendo cromossomos são combinadas aos pares durante a... more
Neste trabalho didático, é apresentada uma alternativa para o ensino das alterações cromossômicas numéricas, as aneuploidias. A atividade é proposta na forma de um jogo cujas cartas, contendo cromossomos são combinadas aos pares durante a montagem do cariótipo, originando assim diferentes aneuploidias. Além de proporcionar um momento de descontração, o jogo também favorece a participação ativa dos alunos na construção do conhecimento e no desenvolvimento de estratégias para dinamizar a aplicação.
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A atividade proposta tem como função conectar conteúdos ministrados na graduação e pós-graduação das áreas de genética geral, citogenética, genética de populações, evolução, sistemática filogenética e zoologia. Utilizamos um modelo de... more
A atividade proposta tem como função conectar conteúdos ministrados na graduação e pós-graduação das áreas de genética geral, citogenética, genética de populações, evolução, sistemática filogenética e zoologia. Utilizamos um modelo de evolução cromossômica para acessar questões sobre os mecanismos envolvidos na reorganização dos genomas ao longo da diversificação das espécies. Resolvendo uma situação problema os discentes terão a possibilidade de verificar como cada tipo de marcador cromossômico pode ser empregado para solucionar questões taxonômicas e observar que tipos de rearranjos cromossômicos podem ocorrer durante a evolução.
Research Interests: Humanities and Biology
Invasive rats and mice typically arrive in novel regions through unintentional transportation aboard ocean-going vessels, which makes seaports the principal point of entry of these species. In this innovative study, we modeled the spatial... more
Invasive rats and mice typically arrive in novel regions through unintentional transportation aboard ocean-going vessels, which makes seaports the principal point of entry of these species. In this innovative study, we modeled the spatial distribution and abundance of two invasive rodent species (Rattus norvegicus and Mus musculus) in Paranaguá, a port city, in southern Brazil whose original vegetation cover was Atlantic Forest. Occupancy and abundance were estimated using live and pitfall traps during nine field campaigns (4,214 trap.nights) at 18 sampling sites distributed along a gradient of habitat between the port and the forest. The abundance and occupancy of both species were affected negatively by the percentage of forest cover within a radius of 1,000 m. The occupancy of M. musculus was also affected by the abundance of buildings within a radius of 100 m and by the distance to the nearest forest edge. The occupancy of R. norvegicus was related positively to the proximity of...
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We analyzed cito and taxonomically specimens (6M 8F)of Akodon montensis from São Francisco do Sul island – SC. The island is situated in the region of phytoecological Rain Forest (Atlantic Forest), composed mainly by the sub-formations... more
We analyzed cito and taxonomically specimens (6M 8F)of Akodon montensis from São Francisco do Sul island – SC. The island is situated in the region of phytoecological Rain Forest (Atlantic Forest), composed mainly by the sub-formations lowlands, sub-montane and sandbanks.The specimens were collected in life traps types Sherman and Tomahawk, and the metaphases obtained through direct preparation of bone marrow and subjected to different staining techniques: common (Giemsa), GTG, CBG and Ag-NOR.The most common karyotype in Akodon montensis (2n = 24; NFA = 42) consists, among autosomes, with 10 pairs of meta-submetacentric chromosomes (1 to 9 and 11) and a pair of acrocentric (10), and between sex, X and Y acrocentric.Partial analysis of this sample, common staining showed a variation in the number of chromosomes (2n = 24 to 27) and the arms of autosomes (NFA = 42 to 48) due to occurrence from 0 to 3 supernumerary chromosomes which: a) five female (2n = 24; NFA = 42); b) three females ...
Bibimys is a genus still poorly known in its basic aspects, such as systematics, natural history and geographic distribution. In Brazil, only Bibimys labiosus is recorded, with occasional records spread in time and space. This paper... more
Bibimys is a genus still poorly known in its basic aspects, such as systematics, natural history and geographic distribution. In Brazil, only Bibimys labiosus is recorded, with occasional records spread in time and space. This paper presents four new localities of the species in Araucaria Forest, Paraná state, extending its distribution 150 km southwards. Additionally, a map of the known distribution of the species in Brazil and in Araucaria Forest is provided. The records point toward the lack of knowledge about basic aspects of the small non-flying mammal fauna in the plateau regions of Brazil.
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Research Interests:
Juliomys ossitenuis was previously known from the Atlantic Forest of southeastern Brazil, where it occurs in Dense Ombrophilous Forest. The record of the species in Floresta Nacional de Piraí do Sul, in Paraná state, confirmed by... more
Juliomys ossitenuis was previously known from the Atlantic Forest of southeastern Brazil, where it occurs in Dense Ombrophilous Forest. The record of the species in Floresta Nacional de Piraí do Sul, in Paraná state, confirmed by morphological and cytogenetic analyses, extends its distribution about 200 km southwards. This is the first record of the species in the state and in an area of Mixed Ombrophilous Forest (Araucaria Forest).
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Unexpected chromosomal alterations in Tayassu tajacu
O genero Akodon e um dos mais diversificados da America do Sul, com 41 especies reconhecidas, e se distribui ao longo de todo o continente, indo da Colombia ate a Argentina. As especies desse genero sao morfologicamente muito similares, e... more
O genero Akodon e um dos mais diversificados da America do Sul, com 41 especies reconhecidas, e se distribui ao longo de todo o continente, indo da Colombia ate a Argentina. As especies desse genero sao morfologicamente muito similares, e por isso a citogenetica e uma ferramenta chave para a identificacao especifica. O objeto deste estudo, a especie A. montensis , possui cariotipo padrao com 2n=24 e NA=42. De modo geral o conjunto cromossomico de uma especie deve se manter constante a fim de evitar erros na formacao dos gametas e garantir assim a viabilidade da prole, porem variacoes morfologicas e ate numericas nesse conjunto podem ser encontradas com certa frequencia. O objetivo desse trabalho foi avaliar as variacoes cromossomicas encontradas em individuos de Akodon montensis coletados na Floresta Nacional de Pirai do Sul, Parana. Foram coletados 15 individuos de A. montensis , sendo sete femeas e oito machos. Foram identificadas alteracoes morfologicas nos cromossomos X, sendo a...
Seaports are introduction hotspots for invasive alien species (IAS). This is especially true for rodents, which have accompanied humans around the globe since the earliest days of ocean-going voyages. The rapid spread of IAS soon after... more
Seaports are introduction hotspots for invasive alien species (IAS). This is especially true for rodents, which have accompanied humans around the globe since the earliest days of ocean-going voyages. The rapid spread of IAS soon after arrival in a new environment is facilitated by further human-mediated transport or landscape features, like roads. By measuring genetic diversity and structure to investigate dispersal pathways, we gained insight into the transport, spread and establishment stages of a biological invasion, leveraging the most common rodent species (R. norvegicus) in this setting. We characterized the genetic structure of three Norway rat populations along a busy industrial road used by trucks to access the Port area in Paranaguá city (Brazil). A total of 71 rats were genotyped using 11 microsatellite markers. The results revealed a pattern of gene flow contrary to the expected stepping-stone model along the linear transect, with the two furthest apart populations bein...
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Bird chromosomes, which have been investigated scientifically for more than a century, present a number of unique features. In general, bird karyotypes have a high diploid number (2n) of typically around 80 chromosomes that are divided... more
Bird chromosomes, which have been investigated scientifically for more than a century, present a number of unique features. In general, bird karyotypes have a high diploid number (2n) of typically around 80 chromosomes that are divided into macro- and microchromosomes. In recent decades, FISH studies using whole chromosome painting probes have shown that the macrochromosomes evolved through both inter- and intrachromosomal rearrangements. However, chromosome painting data are available for only a few bird species, which hinders a more systematic approach to the understanding of the evolutionary history of the enigmatic bird karyotype. Thus, we decided to create an innovative database through compilation of the cytogenetic data available for birds, including chromosome numbers and the results of chromosome painting with chicken (Gallus gallus) probes. The data were obtained through an extensive literature review, which focused on cytogenetic studies published up to 2019. In the first...
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B chromosomes are supernumerary chromosomes found in the karyotypes of approximately 15% of all eukaryotic species. They present parasitic behavior and do not follow the standard Mendelian pattern of inheritance, resulting in an imbalance... more
B chromosomes are supernumerary chromosomes found in the karyotypes of approximately 15% of all eukaryotic species. They present parasitic behavior and do not follow the standard Mendelian pattern of inheritance, resulting in an imbalance in gametogenesis. The evolutionary dynamics of B chromosomes is still unknown for many species, but studies indicate that the accumulation of repetitive sequences plays an important role in the differentiation of these elements. We analyzed morphology, frequency, and possible homologies amongst different B chromosomes found in an isolated Akodon montensis population in southern Brazil. Repetitive sequences (18S, 5S rDNA and telomeric sequences) were used to test for their accumulation on the supernumerary chromosomes and describe their localization in the species. The results indicate 4 different B chromosome morphotypes, and DNA libraries were generated for 3 of them. 18S rDNA was labelled polymorphically, except in the B chromosomes, whereas the ...
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Research Interests:
Kingfishers comprise about 115 species of the family Alcedinidae, and are an interesting group for cytogenetic studies, for they are among birds with most heterogeneous karyotypes. However, cytogenetics knowledge in Kingfishers is... more
Kingfishers comprise about 115 species of the family Alcedinidae, and are an interesting group for cytogenetic studies, for they are among birds with most heterogeneous karyotypes. However, cytogenetics knowledge in Kingfishers is extremely limited. Thus, the aim of this study was to describe the karyotype structure of the Ringed Kingfisher ( Linnaeus, 1766) and Green Kingfisher ( Gmelin, 1788) and also compare them with related species in order to identify chromosomal rearrangements. The Ringed Kingfisher presented 2n = 84 and the Green Kingfisher had 2n = 94. The increase of the chromosome number in the Green Kingfisher possibly originated by centric fissions in macrochromosomes. In addition, karyotype comparisons in Alcedinidae show a heterogeneity in the size and morphology of macrochromosomes, and chromosome numbers ranging from 2n = 76 to 132. Thus, it is possible chromosomal fissions in macrochromosomes resulted in the increase of the diploid number, whereas chromosome fusion...
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Juliomys ossitenuis was previously known from the Atlantic Forest of southeastern Brazil, where it occurs in Dense Ombrophilous Forest. The record of the species in Flo - resta Nacional de Piraí do Sul, in Paraná state, confirmed by... more
Juliomys ossitenuis was previously known from the Atlantic Forest of southeastern Brazil, where it occurs in Dense Ombrophilous Forest. The record of the species in Flo - resta Nacional de Piraí do Sul, in Paraná state, confirmed by morphological and cytogenetic analyses, extends its distribu- tion about 200 km southwards. This is the first record of the species in the state and in an area of Mixed Ombrophilous Forest (Araucaria Forest).
Research Interests:
Seaports are introduction hotspots for invasive alien species (IAS). This is especially true for rodents, which have accompanied humans around the globe since the earliest days of ocean-going voyages. The rapid spread of IAS soon after... more
Seaports are introduction hotspots for invasive alien species (IAS). This is especially true for rodents, which have accompanied humans around the globe since the earliest days of ocean-going voyages. The rapid spread of IAS soon after arrival in a new environment is facilitated by further human-mediated transport or landscape features, like roads. By measuring genetic diversity and structure to investigate dispersal pathways, we gained insight into the transport, spread and establishment stages of a biological invasion, leveraging the most common rodent species (R. norvegicus) in this setting. We characterized the genetic structure of three Norway rat populations along a busy industrial road used by trucks to access the Port area in Paranaguá city (Brazil). A total of 71 rats were genotyped using 11 microsatellite markers. The results revealed a pattern of gene flow contrary to the expected stepping-stone model along the linear transect, with the two furthest apart populations being clustered together. We hypothesize that the observed outcome is explained by natural dispersal along the corridor being lower than human-mediated transport. The sampled area furthest from the port is a gas station frequented by trucks which are considered the most likely mode of transportation. In terms of management strategies, we suggest more emphasis should be put on cargo surveillance to lower the risk of Norway rat dispersal, not only for biosecurity, but also for sanitary reasons, as this port is a major grain trading point.
Research Interests:
Bibimys is a genus still poorly known in its basic aspects, such as systematics, natural history and geographic distribution. In Brazil, only Bibimys labiosus is recorded, with occasional records spread in time and space. This paper... more
Bibimys is a genus still poorly known in its basic aspects, such as systematics, natural history and geographic distribution. In Brazil, only Bibimys labiosus is recorded, with occasional records spread in time and space. This paper presents four new localities of the species in Araucaria Forest, Paraná state, extending its distribution 150 km southwards. Additionally, a map of the known distribution of the species in Brazil and in Araucaria Forest is provided. The records point toward the lack of knowledge about basic aspects of the small non-flying mammal fauna in the plateau regions of Brazil.
Research Interests:
Juliomys ossitenuis was previously known from the Atlantic Forest of southeastern Brazil, where it occurs in Dense Ombrophilous Forest. The record of the species in Flo - resta Nacional de Piraí do Sul, in Paraná state,... more
Juliomys ossitenuis was previously known from
the Atlantic Forest of southeastern Brazil, where it occurs in
Dense Ombrophilous Forest. The record of the species in Flo -
resta Nacional de Piraí do Sul, in Paraná state, confirmed by
morphological and cytogenetic analyses, extends its distribu-
tion about 200 km southwards. This is the first record of the
species in the state and in an area of Mixed Ombrophilous
Forest (Araucaria Forest).
the Atlantic Forest of southeastern Brazil, where it occurs in
Dense Ombrophilous Forest. The record of the species in Flo -
resta Nacional de Piraí do Sul, in Paraná state, confirmed by
morphological and cytogenetic analyses, extends its distribu-
tion about 200 km southwards. This is the first record of the
species in the state and in an area of Mixed Ombrophilous
Forest (Araucaria Forest).
Research Interests:
Wild animals have been used as bioindicators in situations in which the environment was exposed to chemical agents. In general, chemical agents may induce chromosomal aberrations, such as breaks and gaps. The peccary, Tayassu tajacu is a... more
Wild animals have been used as bioindicators in situations in which the environment was exposed to chemical agents. In general, chemical agents may induce chromosomal aberrations, such as breaks and gaps. The peccary, Tayassu tajacu is a pig relative that exhibits a very stable karyotype with the only described alterations being of the form of the X chromosome. Chromosomal gaps and breaks were observed at high frequencies during cytogenetics analyses. These alterations were observed in the chromosomes autossomics. Reviews of the literature and of the data described herein suggests that an vermifuge, the ivermectin base, was the most likely cause of these chromosomal alterations.
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We established chromosome homology maps between Mus musculus (MMU) and five species of the Akodontini tribe, Akodon cursor (2n = 14, 15 and 16), A. montensis (2n = 24), A. paranaensis (2n = 44), A. serrensis (2n = 46) and Oligoryzomys... more
We established chromosome homology maps between Mus musculus (MMU) and five species of the Akodontini tribe, Akodon cursor (2n = 14, 15 and 16), A. montensis (2n = 24), A. paranaensis (2n = 44), A. serrensis (2n = 46) and Oligoryzomys flavescens (2n = 66) by Zoo-FISH (fluorescence in situ hybridization) using mouse chromosome-specific probes. The aims of this study were (1) to detect the chromosomal rearrangements responsible for the karyotype variation in this tribe and (2) to reconstruct the phylogenetic relationships among these species. We observed four common syntenic associations of homologous chromosome segments, of which the MMU 7/19 has been described previously in other rodents from Africa, Asia and Europe, and might represent a phylogenetic link between the Old World and Neotropical rodents. The remaining three associations (3/18, 6/12 and 8/13) have been observed exclusively in Neotropical rodents so far, which at present can be considered synapomorphic traits of this group. Five further mouse chromosomes (MMU 4, 9, 14, 18 and 19) were each found evolutionarily conserved as a separate syntenic unit. Our phylogenetic analysis using parsimony and heuristic search detected one consistent group, separating the Akodontini from other rodents.