sumathy joseph

Pedalium murex has been an integral part of many important herbal formulations used in traditional systems of medicine. The present study was carried out to evaluate the antiproliferative activity of the methanolic fruit extract of P. murex using various solvents in the order of petroleum ether, chloroform, ethyl acetate, methanol and hydro-alcohol. The phytochemical screening of the extracts showed the presence of different bio- active compounds namely flavonoids, saponins, alkaloids, glycosides, tannins, carbohydrates and phenols. TLC analysis further confirms the presence of flavonoids and phenols by their respective Rf values. The fruit extract was also found to contain significant amount of vitamin C. Free radical scavenging activity of the extracts was also studied using DPPH as the substrate since plant phenolic compounds act as primary antioxidant of free radical scavengers. Flavonoids and vitamin C are potent antioxidants, can act as effective anti-cancer agents by preventi...

Objective: The objective of the study was to evaluate the α-amylase inhibitory activity against porcine pancreatic amylase (PPA) and antioxidant potential of ethanolic leaf extract of C.bonplandianum in- vitro. Materials and Methods: The leaf extract was prepared with ethanol, filtered, distilled and stored at -20°C. Preliminary phytochemical screening and quantitative estimation of phenols, flavonoids, tannins and total antioxidant capacity along with free radical scavenging activity (DPPH) was carried out. Different concentrations (10,20,40,60,80,100μg/ml) of the extract was subjected to α-amylase inhibitory activity using soluble starch as substrate and the IC 50 value was calculated. Compounds responsible for these activities were identified by GC-MS with the inbuilt libraries (NIST - 11). Results: Phytochemical screening revealed the presence of phytoconstituents like phenols, flavonoids, alkaloids, saponins, terpenoids, etc. Presence of polyphenols (41.26±0.39 μg/ml), flavonoi...

Increase in adiposity is associated with obesity and obesity-related complications. Syringic acid (SA), a phenolic acid facilitates oxidation, polymerization, and condensation reactions, may function as an effective anti-adipogenic agent. This study examined whether SA inhibits adipogenesis in 3T3-L1 preadipocytes by inhibiting differentiation and controlling lipid accumulation. Ten days of SA treatment suppressed differentiation of 3T3-L1 preadipocyte to mature adipocytes in a concentration-dependent manner as determined by Oil Red O staining. Suppression of adipogenic differentiation by SA was primarily from inhibition of mitotic clonal expansion during the early stages of differentiation. Treatment of adipocytes with SA reduced triglyceride production (lipogenesis) and increased glycerol release (lipolysis). The addition of SA to the adipocytes attenuated in vitro ROS production. These results indicate that SA inhibits the adipogenesis of 3T3-L1 preadipocytes, reduces lipid accumulation, and attenuates ROS accumulation, suggesting for the first time that SA has an anti-obesity effect in vitro.