Investigative Ophthalmology & Visual Science, Jun 16, 2013
Purpose Aquaporin‐1 (AQP1) is involved in cell migration and proliferation. This study aims to in... more Purpose Aquaporin‐1 (AQP1) is involved in cell migration and proliferation. This study aims to investigate AQP1 expression in membranes from proliferative vitreoretinopathy (PVR) and epiretinal membranes (ERM)
The stimulatory effect of vasoactive intestinal peptide (VIP) and analogues on [Ca2+]ihas been in... more The stimulatory effect of vasoactive intestinal peptide (VIP) and analogues on [Ca2+]ihas been investigated in chinese hamster ovary (CHO) cells stably transfected with the reporter gene aequorin, and expressing either the human VPAC1or VPAC2receptor in absence or in presence of the Gα16 . In cells that were not transfected with Gα16 and expressed a similar density of receptors, the VIP
Purpose Aquaporin‐1 (AQP1) is involved in cell migration and proliferation. This study aims to in... more Purpose Aquaporin‐1 (AQP1) is involved in cell migration and proliferation. This study aims to investigate AQP1 expression in membranes from proliferative vitreoretinopathy (PVR) and epiretinal membranes (ERM)Methods 20 Membranes PVR and ERM were collected following eye surgery from 20 eyes from 20 patients. AQP1 mRNA and protein expression were determined by RT‐qPCR and immunofluorescence in both PVR and ERMResults AQP1 mRNA and protein were expressed in both PVR and ERM as shown by both RT‐qPCR and immunofluorescence. Moreover, AQP1 protein expression was heterogeneous among and between PVR and ERM, and was highly colocalized with alpha‐smooth muscle actin (SMA or Acta2) and slightly colocalized with glial fibrillary acidic protein (GFAP).Conclusion AQP1 mRNA and protein were expressed in membranes from PVR and in ERM. Due to the absence of SMA and GFAP colocalization, it is likely that AQP1 is expressed by at least two distinct cells types. AQP1 might play a role in cell migratio...
Experimental and Clinical Endocrinology & Diabetes, Jul 16, 2009
Abstract Thyroid-stimulating hormone (TSH) receptor has been cloned by a strategy exploiting its ... more Abstract Thyroid-stimulating hormone (TSH) receptor has been cloned by a strategy exploiting its belonging to the superfamily of G-protein-coupled receptors. Comparison with the luteinizing hormone and follicle-stimulating hormone receptors shows that the glycoprotein hormone receptors constitute a subfamily of G-protein-coupled receptors with a large extracellular domain probably responsible for hormone binding. The recombinant human TSH receptor stably expressed in cell lines provides the tool to identify the targets ...
Investigative Ophthalmology & Visual Science, Jun 16, 2013
Purpose Aquaporin‐1 (AQP1) is involved in cell migration and proliferation. This study aims to in... more Purpose Aquaporin‐1 (AQP1) is involved in cell migration and proliferation. This study aims to investigate AQP1 expression in membranes from proliferative vitreoretinopathy (PVR) and epiretinal membranes (ERM)
The stimulatory effect of vasoactive intestinal peptide (VIP) and analogues on [Ca2+]ihas been in... more The stimulatory effect of vasoactive intestinal peptide (VIP) and analogues on [Ca2+]ihas been investigated in chinese hamster ovary (CHO) cells stably transfected with the reporter gene aequorin, and expressing either the human VPAC1or VPAC2receptor in absence or in presence of the Gα16 . In cells that were not transfected with Gα16 and expressed a similar density of receptors, the VIP
Purpose Aquaporin‐1 (AQP1) is involved in cell migration and proliferation. This study aims to in... more Purpose Aquaporin‐1 (AQP1) is involved in cell migration and proliferation. This study aims to investigate AQP1 expression in membranes from proliferative vitreoretinopathy (PVR) and epiretinal membranes (ERM)Methods 20 Membranes PVR and ERM were collected following eye surgery from 20 eyes from 20 patients. AQP1 mRNA and protein expression were determined by RT‐qPCR and immunofluorescence in both PVR and ERMResults AQP1 mRNA and protein were expressed in both PVR and ERM as shown by both RT‐qPCR and immunofluorescence. Moreover, AQP1 protein expression was heterogeneous among and between PVR and ERM, and was highly colocalized with alpha‐smooth muscle actin (SMA or Acta2) and slightly colocalized with glial fibrillary acidic protein (GFAP).Conclusion AQP1 mRNA and protein were expressed in membranes from PVR and in ERM. Due to the absence of SMA and GFAP colocalization, it is likely that AQP1 is expressed by at least two distinct cells types. AQP1 might play a role in cell migratio...
Experimental and Clinical Endocrinology & Diabetes, Jul 16, 2009
Abstract Thyroid-stimulating hormone (TSH) receptor has been cloned by a strategy exploiting its ... more Abstract Thyroid-stimulating hormone (TSH) receptor has been cloned by a strategy exploiting its belonging to the superfamily of G-protein-coupled receptors. Comparison with the luteinizing hormone and follicle-stimulating hormone receptors shows that the glycoprotein hormone receptors constitute a subfamily of G-protein-coupled receptors with a large extracellular domain probably responsible for hormone binding. The recombinant human TSH receptor stably expressed in cell lines provides the tool to identify the targets ...
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