Common methods employed in assisted reproduction technology (ART) include intracytoplasmic
sperm ... more Common methods employed in assisted reproduction technology (ART) include intracytoplasmic sperm injection (ICSI) with an unspecified level of sperm DNA fragmentation (SDF) and preimplantation genetic diagnosis (PGD). The aim of this study was to investigate the impact of SDF on human preimplantation embryo development and the incidence of apoptosis following a single blastomere biopsy. Using sperm chromatin dispersion (SCD) to assess SDF, a total of 20 processed semen samples were categorized into two groups; group I: SDF 30% and group II: SDF430%. After ICSI, fertilization, cleavage, and embryo quality score were assessed. A single blastomere was biopsied from day 3 embryos and development was monitored on day 4. The frequency of apoptosis in biopsied embryos was assayed by TUNEL and the level of BCL-2, BAX, hsa-mir-15a, and hsa-mir-16-1 were assessed by quantitative real-time polymerase chain reaction (qRT-PCR). SCD was found to be negatively correlated with sperm motility and normal form spermatozoa (p50.05). The rate of fertilization, cleavage, and embryo quality score were not significantly different between the two groups (all p40.05). SDF430% had no negative effect on potential development and did not increase the proportion of apoptotic cells and the level of apoptosis-related genes and microRNAs (miRNAs) in group II vs. group I (p40.05). It appears that at the levels assessed paternal genome damage had little if any negative effect on preimplantaton embryo development and apoptosis following single blastomere biopsy. This may reflect the selection of morphologically normal sperm for ICSI and the repair capacity of the oocyte.
Variations in arterial anatomy are less frequent, contrary to the venous system, and most
of thes... more Variations in arterial anatomy are less frequent, contrary to the venous system, and most of these variations affect visceral arteries. Variations in the brachial artery are the most frequently reported and so far a minimum of six different patterns have been described. The most common of these patterns is the superficial brachial artery, which lies superficially to the median nerve. Much less prevalent is the high origin of the radial artery (brachioradial artery) or the existence of a doubled brachial artery (accessory brachial artery). The current study presents a pattern of brachial artery variation which was previously undescribed. During dissection of the right upper limb of a 50 year-old male embalmed cadaver, the bifurcation of the brachial artery in the proximal portion of the middle third of the arm was observed. In this case, the medial branch reaches the medial aspect of the arm, posterior to the median nerve. Afterwards, this medial branch redirects laterally and crosses the median nerve again, this time lying anterior to the nerve till it reaches the lateral aspect of the arm. At the elbow level, the medial branch originates from the radial artery. The lateral branch of the brachial artery remains lateral to the median nerve and continues as ulnar artery and originates from the interosseous artery. It was also observed that the left brachial artery was smaller in size, and bifurcated high in the arm into the superficial radial and ulnar arteries. It was also interesting to note that the common interosseous artery was originated from the left radial artery in the cubital fossa, which descended deep to pronator teres where it was divided into the anterior and posterior interosseous arteries. These variations are discussed comprehensively and compared with the previous reports. Also, it is asserted how clinically the findings are significant.
Introduction: SOX9 is a transcriptional activator which is necessary for chondrogenesis. SOX6
are... more Introduction: SOX9 is a transcriptional activator which is necessary for chondrogenesis. SOX6 are closely related to DNA-binding proteins that critically enhance its function. Therefore, to carry out the growth plate chondrocyte differentiation program, SOX9 and SOX6 collaborate genomewide. Chondrocyte differentiation is also known to be promoted by glucocorticoids through unknown molecular mechanisms. Methods: We investigated the effects of asynthetic glucocorticoid, dexamethasone (DEX), on SOX9 gene expression in chondrocytes. Results: SOX9 mRNA was expressed at high levels in these chondrocytes. Treatment with DEX resulted in enhancement of SOX9 mRNA expression. The DEX effect was dose dependent (0·5 nM and 1 nM). Conclusion: RT-PCR analysis revealed that DEX also enhanced the levels of SOX9 expression. It was observed that DEX had enhancing effect only on SOX9; the expression level was low for SOX6. It can thus be concluded that chondrocyte differentiation can be promoted by DEX via SOX9 enhancement
Aim
SHARIF-HMIS is a new inertial sensor designed for movement analysis. The aim of the presentst... more Aim SHARIF-HMIS is a new inertial sensor designed for movement analysis. The aim of the presentstudy wasto assess the inter-tester and intra-testerre liability of some kinematic parameters in different lumbar motions making use of this sensor.
Materials and Methods 24 healthy persons and 28 patients with low back pain participated in the current reliability study. The test was performed in five different lumbar motions consisting of lumbar flexion in 0, 15, and 30 degrees in the right and left directions. For measuring inter-tester reliability, all the tests were carried out twice on the same day separately by two physiotherapists. Intra-tester reliability was assessed by reproducing the tests after 3 days by the same physiotherapist.
Findings The present study revealed satisfactory inter- and intra-tester reliability indices in different positions. ICCs for intra-tester reliability ranged from 0.65 to 0.98 and 0.59 to 0.81 for healthy and patient participants, respectively. Also, ICCs for inter-tester reliability ranged from 0.65 to 0.92 for the healthy and 0.65 to 0.87 for patient participants.
Conclusion In general, it can be inferred from the results that measuring the kinematic parameters in lumbar movements using inertial sensors enjoys acceptable reliability.
Introduction: Multiple sclerosis (MS) is a disease of the immune system: it attacks the myelin
ar... more Introduction: Multiple sclerosis (MS) is a disease of the immune system: it attacks the myelin around the axons and leaves them exposed. Destruction of myelin weakens the electrical conduction of ions and thus leads to a lack of communication in the nervous system. Methods: In the present study, we constructed recombinant plasmid and then transformed to E. coli cell. The colonies containing plasmid were selected by Colony PCR. Enzyme digestion and sequencing were utilized to approve the accuracy of the extracted plasmid of these clones. Recombinant plasmid transfect in to mesanchymal stem cells. Results: Plasmid was verified correctly. After transfection, the transcription of MOG gene and the expression of MOG protein were proved by RT-PCR, western blotting and Elisa. Conclusion: Plasmid was constructed correctly and mesenchyme stem cells were successfully transfected by transfection and protein can be expressed well, setting a proper foundation for the future studies on the transplantation of gene modified mesanchymal stem cells in order to promote Multiple sclerosis.
Common methods employed in assisted reproduction technology (ART) include intracytoplasmic
sperm ... more Common methods employed in assisted reproduction technology (ART) include intracytoplasmic sperm injection (ICSI) with an unspecified level of sperm DNA fragmentation (SDF) and preimplantation genetic diagnosis (PGD). The aim of this study was to investigate the impact of SDF on human preimplantation embryo development and the incidence of apoptosis following a single blastomere biopsy. Using sperm chromatin dispersion (SCD) to assess SDF, a total of 20 processed semen samples were categorized into two groups; group I: SDF 30% and group II: SDF430%. After ICSI, fertilization, cleavage, and embryo quality score were assessed. A single blastomere was biopsied from day 3 embryos and development was monitored on day 4. The frequency of apoptosis in biopsied embryos was assayed by TUNEL and the level of BCL-2, BAX, hsa-mir-15a, and hsa-mir-16-1 were assessed by quantitative real-time polymerase chain reaction (qRT-PCR). SCD was found to be negatively correlated with sperm motility and normal form spermatozoa (p50.05). The rate of fertilization, cleavage, and embryo quality score were not significantly different between the two groups (all p40.05). SDF430% had no negative effect on potential development and did not increase the proportion of apoptotic cells and the level of apoptosis-related genes and microRNAs (miRNAs) in group II vs. group I (p40.05). It appears that at the levels assessed paternal genome damage had little if any negative effect on preimplantaton embryo development and apoptosis following single blastomere biopsy. This may reflect the selection of morphologically normal sperm for ICSI and the repair capacity of the oocyte.
Variations in arterial anatomy are less frequent, contrary to the venous system, and most
of thes... more Variations in arterial anatomy are less frequent, contrary to the venous system, and most of these variations affect visceral arteries. Variations in the brachial artery are the most frequently reported and so far a minimum of six different patterns have been described. The most common of these patterns is the superficial brachial artery, which lies superficially to the median nerve. Much less prevalent is the high origin of the radial artery (brachioradial artery) or the existence of a doubled brachial artery (accessory brachial artery). The current study presents a pattern of brachial artery variation which was previously undescribed. During dissection of the right upper limb of a 50 year-old male embalmed cadaver, the bifurcation of the brachial artery in the proximal portion of the middle third of the arm was observed. In this case, the medial branch reaches the medial aspect of the arm, posterior to the median nerve. Afterwards, this medial branch redirects laterally and crosses the median nerve again, this time lying anterior to the nerve till it reaches the lateral aspect of the arm. At the elbow level, the medial branch originates from the radial artery. The lateral branch of the brachial artery remains lateral to the median nerve and continues as ulnar artery and originates from the interosseous artery. It was also observed that the left brachial artery was smaller in size, and bifurcated high in the arm into the superficial radial and ulnar arteries. It was also interesting to note that the common interosseous artery was originated from the left radial artery in the cubital fossa, which descended deep to pronator teres where it was divided into the anterior and posterior interosseous arteries. These variations are discussed comprehensively and compared with the previous reports. Also, it is asserted how clinically the findings are significant.
Introduction: SOX9 is a transcriptional activator which is necessary for chondrogenesis. SOX6
are... more Introduction: SOX9 is a transcriptional activator which is necessary for chondrogenesis. SOX6 are closely related to DNA-binding proteins that critically enhance its function. Therefore, to carry out the growth plate chondrocyte differentiation program, SOX9 and SOX6 collaborate genomewide. Chondrocyte differentiation is also known to be promoted by glucocorticoids through unknown molecular mechanisms. Methods: We investigated the effects of asynthetic glucocorticoid, dexamethasone (DEX), on SOX9 gene expression in chondrocytes. Results: SOX9 mRNA was expressed at high levels in these chondrocytes. Treatment with DEX resulted in enhancement of SOX9 mRNA expression. The DEX effect was dose dependent (0·5 nM and 1 nM). Conclusion: RT-PCR analysis revealed that DEX also enhanced the levels of SOX9 expression. It was observed that DEX had enhancing effect only on SOX9; the expression level was low for SOX6. It can thus be concluded that chondrocyte differentiation can be promoted by DEX via SOX9 enhancement
Aim
SHARIF-HMIS is a new inertial sensor designed for movement analysis. The aim of the presentst... more Aim SHARIF-HMIS is a new inertial sensor designed for movement analysis. The aim of the presentstudy wasto assess the inter-tester and intra-testerre liability of some kinematic parameters in different lumbar motions making use of this sensor.
Materials and Methods 24 healthy persons and 28 patients with low back pain participated in the current reliability study. The test was performed in five different lumbar motions consisting of lumbar flexion in 0, 15, and 30 degrees in the right and left directions. For measuring inter-tester reliability, all the tests were carried out twice on the same day separately by two physiotherapists. Intra-tester reliability was assessed by reproducing the tests after 3 days by the same physiotherapist.
Findings The present study revealed satisfactory inter- and intra-tester reliability indices in different positions. ICCs for intra-tester reliability ranged from 0.65 to 0.98 and 0.59 to 0.81 for healthy and patient participants, respectively. Also, ICCs for inter-tester reliability ranged from 0.65 to 0.92 for the healthy and 0.65 to 0.87 for patient participants.
Conclusion In general, it can be inferred from the results that measuring the kinematic parameters in lumbar movements using inertial sensors enjoys acceptable reliability.
Introduction: Multiple sclerosis (MS) is a disease of the immune system: it attacks the myelin
ar... more Introduction: Multiple sclerosis (MS) is a disease of the immune system: it attacks the myelin around the axons and leaves them exposed. Destruction of myelin weakens the electrical conduction of ions and thus leads to a lack of communication in the nervous system. Methods: In the present study, we constructed recombinant plasmid and then transformed to E. coli cell. The colonies containing plasmid were selected by Colony PCR. Enzyme digestion and sequencing were utilized to approve the accuracy of the extracted plasmid of these clones. Recombinant plasmid transfect in to mesanchymal stem cells. Results: Plasmid was verified correctly. After transfection, the transcription of MOG gene and the expression of MOG protein were proved by RT-PCR, western blotting and Elisa. Conclusion: Plasmid was constructed correctly and mesenchyme stem cells were successfully transfected by transfection and protein can be expressed well, setting a proper foundation for the future studies on the transplantation of gene modified mesanchymal stem cells in order to promote Multiple sclerosis.
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sperm injection (ICSI) with an unspecified level of sperm DNA fragmentation (SDF) and
preimplantation genetic diagnosis (PGD). The aim of this study was to investigate the impact of
SDF on human preimplantation embryo development and the incidence of apoptosis following a
single blastomere biopsy. Using sperm chromatin dispersion (SCD) to assess SDF, a total of 20
processed semen samples were categorized into two groups; group I: SDF 30% and group II:
SDF430%. After ICSI, fertilization, cleavage, and embryo quality score were assessed. A single
blastomere was biopsied from day 3 embryos and development was monitored on day 4. The
frequency of apoptosis in biopsied embryos was assayed by TUNEL and the level of BCL-2, BAX,
hsa-mir-15a, and hsa-mir-16-1 were assessed by quantitative real-time polymerase chain reaction
(qRT-PCR). SCD was found to be negatively correlated with sperm motility and normal form
spermatozoa (p50.05). The rate of fertilization, cleavage, and embryo quality score were not
significantly different between the two groups (all p40.05). SDF430% had no negative effect on
potential development and did not increase the proportion of apoptotic cells and the level of
apoptosis-related genes and microRNAs (miRNAs) in group II vs. group I (p40.05). It appears that
at the levels assessed paternal genome damage had little if any negative effect on preimplantaton
embryo development and apoptosis following single blastomere biopsy. This may reflect the
selection of morphologically normal sperm for ICSI and the repair capacity of the oocyte.
of these variations affect visceral arteries. Variations in the brachial artery are the most
frequently reported and so far a minimum of six different patterns have been described. The
most common of these patterns is the superficial brachial artery, which lies superficially to
the median nerve. Much less prevalent is the high origin of the radial artery (brachioradial
artery) or the existence of a doubled brachial artery (accessory brachial artery). The current
study presents a pattern of brachial artery variation which was previously undescribed.
During dissection of the right upper limb of a 50 year-old male embalmed cadaver, the
bifurcation of the brachial artery in the proximal portion of the middle third of the arm was
observed. In this case, the medial branch reaches the medial aspect of the arm, posterior to
the median nerve. Afterwards, this medial branch redirects laterally and crosses the median
nerve again, this time lying anterior to the nerve till it reaches the lateral aspect of the arm.
At the elbow level, the medial branch originates from the radial artery. The lateral branch
of the brachial artery remains lateral to the median nerve and continues as ulnar artery and
originates from the interosseous artery. It was also observed that the left brachial artery was
smaller in size, and bifurcated high in the arm into the superficial radial and ulnar arteries.
It was also interesting to note that the common interosseous artery was originated from the
left radial artery in the cubital fossa, which descended deep to pronator teres where it was
divided into the anterior and posterior interosseous arteries. These variations are discussed
comprehensively and compared with the previous reports. Also, it is asserted how clinically
the findings are significant.
are closely related to DNA-binding proteins that critically enhance its function. Therefore, to carry
out the growth plate chondrocyte differentiation program, SOX9 and SOX6 collaborate genomewide.
Chondrocyte differentiation is also known to be promoted by glucocorticoids through
unknown molecular mechanisms.
Methods: We investigated the effects of asynthetic glucocorticoid, dexamethasone (DEX), on
SOX9 gene expression in chondrocytes.
Results: SOX9 mRNA was expressed at high levels in these chondrocytes. Treatment with DEX
resulted in enhancement of SOX9 mRNA expression. The DEX effect was dose dependent (0·5
nM and 1 nM).
Conclusion: RT-PCR analysis revealed that DEX also enhanced the levels of SOX9 expression.
It was observed that DEX had enhancing effect only on SOX9; the expression level was low for
SOX6. It can thus be concluded that chondrocyte differentiation can be promoted by DEX via
SOX9 enhancement
SHARIF-HMIS is a new inertial sensor designed for movement analysis. The aim of the presentstudy wasto assess the inter-tester and intra-testerre liability of some kinematic parameters in different lumbar motions making use of this sensor.
Materials and Methods
24 healthy persons and 28 patients with low back pain participated in the current reliability study. The test was performed in five different lumbar motions consisting of lumbar flexion in 0, 15, and 30 degrees in the right and left directions. For measuring inter-tester reliability, all the tests were carried out twice on the same day separately by two physiotherapists. Intra-tester reliability was assessed by reproducing the tests after 3 days by the same physiotherapist.
Findings
The present study revealed satisfactory inter- and intra-tester reliability indices in different positions. ICCs for intra-tester reliability ranged from 0.65 to 0.98 and 0.59 to 0.81 for healthy and patient participants, respectively. Also, ICCs for inter-tester reliability ranged from 0.65 to 0.92 for the healthy and 0.65 to 0.87 for patient participants.
Conclusion
In general, it can be inferred from the results that measuring the kinematic parameters in lumbar movements using inertial sensors enjoys acceptable reliability.
around the axons and leaves them exposed. Destruction of myelin weakens the electrical
conduction of ions and thus leads to a lack of communication in the nervous system.
Methods: In the present study, we constructed recombinant plasmid and then transformed to
E. coli cell. The colonies containing plasmid were selected by Colony PCR. Enzyme digestion
and sequencing were utilized to approve the accuracy of the extracted plasmid of these clones.
Recombinant plasmid transfect in to mesanchymal stem cells.
Results: Plasmid was verified correctly. After transfection, the transcription of MOG gene and the
expression of MOG protein were proved by RT-PCR, western blotting and Elisa.
Conclusion: Plasmid was constructed correctly and mesenchyme stem cells were successfully
transfected by transfection and protein can be expressed well, setting a proper foundation for the
future studies on the transplantation of gene modified mesanchymal stem cells in order to promote
Multiple sclerosis.
Talks
sperm injection (ICSI) with an unspecified level of sperm DNA fragmentation (SDF) and
preimplantation genetic diagnosis (PGD). The aim of this study was to investigate the impact of
SDF on human preimplantation embryo development and the incidence of apoptosis following a
single blastomere biopsy. Using sperm chromatin dispersion (SCD) to assess SDF, a total of 20
processed semen samples were categorized into two groups; group I: SDF 30% and group II:
SDF430%. After ICSI, fertilization, cleavage, and embryo quality score were assessed. A single
blastomere was biopsied from day 3 embryos and development was monitored on day 4. The
frequency of apoptosis in biopsied embryos was assayed by TUNEL and the level of BCL-2, BAX,
hsa-mir-15a, and hsa-mir-16-1 were assessed by quantitative real-time polymerase chain reaction
(qRT-PCR). SCD was found to be negatively correlated with sperm motility and normal form
spermatozoa (p50.05). The rate of fertilization, cleavage, and embryo quality score were not
significantly different between the two groups (all p40.05). SDF430% had no negative effect on
potential development and did not increase the proportion of apoptotic cells and the level of
apoptosis-related genes and microRNAs (miRNAs) in group II vs. group I (p40.05). It appears that
at the levels assessed paternal genome damage had little if any negative effect on preimplantaton
embryo development and apoptosis following single blastomere biopsy. This may reflect the
selection of morphologically normal sperm for ICSI and the repair capacity of the oocyte.
of these variations affect visceral arteries. Variations in the brachial artery are the most
frequently reported and so far a minimum of six different patterns have been described. The
most common of these patterns is the superficial brachial artery, which lies superficially to
the median nerve. Much less prevalent is the high origin of the radial artery (brachioradial
artery) or the existence of a doubled brachial artery (accessory brachial artery). The current
study presents a pattern of brachial artery variation which was previously undescribed.
During dissection of the right upper limb of a 50 year-old male embalmed cadaver, the
bifurcation of the brachial artery in the proximal portion of the middle third of the arm was
observed. In this case, the medial branch reaches the medial aspect of the arm, posterior to
the median nerve. Afterwards, this medial branch redirects laterally and crosses the median
nerve again, this time lying anterior to the nerve till it reaches the lateral aspect of the arm.
At the elbow level, the medial branch originates from the radial artery. The lateral branch
of the brachial artery remains lateral to the median nerve and continues as ulnar artery and
originates from the interosseous artery. It was also observed that the left brachial artery was
smaller in size, and bifurcated high in the arm into the superficial radial and ulnar arteries.
It was also interesting to note that the common interosseous artery was originated from the
left radial artery in the cubital fossa, which descended deep to pronator teres where it was
divided into the anterior and posterior interosseous arteries. These variations are discussed
comprehensively and compared with the previous reports. Also, it is asserted how clinically
the findings are significant.
are closely related to DNA-binding proteins that critically enhance its function. Therefore, to carry
out the growth plate chondrocyte differentiation program, SOX9 and SOX6 collaborate genomewide.
Chondrocyte differentiation is also known to be promoted by glucocorticoids through
unknown molecular mechanisms.
Methods: We investigated the effects of asynthetic glucocorticoid, dexamethasone (DEX), on
SOX9 gene expression in chondrocytes.
Results: SOX9 mRNA was expressed at high levels in these chondrocytes. Treatment with DEX
resulted in enhancement of SOX9 mRNA expression. The DEX effect was dose dependent (0·5
nM and 1 nM).
Conclusion: RT-PCR analysis revealed that DEX also enhanced the levels of SOX9 expression.
It was observed that DEX had enhancing effect only on SOX9; the expression level was low for
SOX6. It can thus be concluded that chondrocyte differentiation can be promoted by DEX via
SOX9 enhancement
SHARIF-HMIS is a new inertial sensor designed for movement analysis. The aim of the presentstudy wasto assess the inter-tester and intra-testerre liability of some kinematic parameters in different lumbar motions making use of this sensor.
Materials and Methods
24 healthy persons and 28 patients with low back pain participated in the current reliability study. The test was performed in five different lumbar motions consisting of lumbar flexion in 0, 15, and 30 degrees in the right and left directions. For measuring inter-tester reliability, all the tests were carried out twice on the same day separately by two physiotherapists. Intra-tester reliability was assessed by reproducing the tests after 3 days by the same physiotherapist.
Findings
The present study revealed satisfactory inter- and intra-tester reliability indices in different positions. ICCs for intra-tester reliability ranged from 0.65 to 0.98 and 0.59 to 0.81 for healthy and patient participants, respectively. Also, ICCs for inter-tester reliability ranged from 0.65 to 0.92 for the healthy and 0.65 to 0.87 for patient participants.
Conclusion
In general, it can be inferred from the results that measuring the kinematic parameters in lumbar movements using inertial sensors enjoys acceptable reliability.
around the axons and leaves them exposed. Destruction of myelin weakens the electrical
conduction of ions and thus leads to a lack of communication in the nervous system.
Methods: In the present study, we constructed recombinant plasmid and then transformed to
E. coli cell. The colonies containing plasmid were selected by Colony PCR. Enzyme digestion
and sequencing were utilized to approve the accuracy of the extracted plasmid of these clones.
Recombinant plasmid transfect in to mesanchymal stem cells.
Results: Plasmid was verified correctly. After transfection, the transcription of MOG gene and the
expression of MOG protein were proved by RT-PCR, western blotting and Elisa.
Conclusion: Plasmid was constructed correctly and mesenchyme stem cells were successfully
transfected by transfection and protein can be expressed well, setting a proper foundation for the
future studies on the transplantation of gene modified mesanchymal stem cells in order to promote
Multiple sclerosis.