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YU187991A - 3-(2)-amino-ali tiol-modifikovani, s fluorescentnom bojom vezani nukleozidi, nukleotidi i oligonukleotidi, postupak za njihovo dobijanje i njihova upotreba - Google Patents

3-(2)-amino-ali tiol-modifikovani, s fluorescentnom bojom vezani nukleozidi, nukleotidi i oligonukleotidi, postupak za njihovo dobijanje i njihova upotreba

Info

Publication number
YU187991A
YU187991A YU187991A YU187991A YU187991A YU 187991 A YU187991 A YU 187991A YU 187991 A YU187991 A YU 187991A YU 187991 A YU187991 A YU 187991A YU 187991 A YU187991 A YU 187991A
Authority
YU
Yugoslavia
Prior art keywords
amino
thiol
oligonucleotides
fluorescent
nucleosides
Prior art date
Application number
YU187991A
Other languages
English (en)
Inventor
S.J. Engel
Original Assignee
Hoechst Aktiengesellschaft
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst Aktiengesellschaft filed Critical Hoechst Aktiengesellschaft
Publication of YU187991A publication Critical patent/YU187991A/sh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

3-(2)-AMINO-ALI TIOL-MODIFIKOVANI, S FLUORESCENTNOM BOJOM VEZANI NUKLEOZIDI, NUKLEOTIDI I OLIGONUKLEOTIDI, POSTUPAK ZA NJIHOVO DOBIJANJE I NJIHOVA UPOTREBA, u 3'- i/ili 2'-položaju nalazeću OH-grupu nukleozida, nukleotida ili oligonukleotida pomoću derivata prevedemo u amino ili tiolnu grupu a potom sjedinimo fluorescentni pigment. Nastale 3'- i/ili 2'-amino i tiolno modificirane nukleozide, nukleotide i oligonukleotide možemo potom da upotrebimo za sintezu prema snopovima u prisustvu šablonsog snopa ili oligonukleotida kao i za detekciju genetskog materijala. Jedino ovi nude prednost, da fluorescentnu oznaku više ne postavimo na 5'-kraju oligonukleotida ili na nukleobazu i time je ne treba uvoditi u hemijsku sintezu kao u dosada poznatim markiranim tehnikama, medjutim pošto poznate i uobičajene metode imaju nedostatak, da možemo da upotrebimo samo neke polimeraze za sintezu, da prihvatljivost trifosfata opadne zbog polimeraza a i osim toga je potreban veći višak supstrata.
YU187991A 1990-12-11 1991-12-02 3-(2)-amino-ali tiol-modifikovani, s fluorescentnom bojom vezani nukleozidi, nukleotidi i oligonukleotidi, postupak za njihovo dobijanje i njihova upotreba YU187991A (sh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE4039488 1990-12-11

Publications (1)

Publication Number Publication Date
YU187991A true YU187991A (sh) 1994-09-09

Family

ID=6420050

Family Applications (1)

Application Number Title Priority Date Filing Date
YU187991A YU187991A (sh) 1990-12-11 1991-12-02 3-(2)-amino-ali tiol-modifikovani, s fluorescentnom bojom vezani nukleozidi, nukleotidi i oligonukleotidi, postupak za njihovo dobijanje i njihova upotreba

Country Status (27)

Country Link
US (3) US5679785A (sh)
EP (1) EP0490281B1 (sh)
JP (1) JP3140127B2 (sh)
KR (1) KR920012107A (sh)
AT (1) ATE142222T1 (sh)
AU (1) AU643856B2 (sh)
BG (1) BG61484B1 (sh)
BR (1) BR9105335A (sh)
CA (1) CA2057475A1 (sh)
CS (1) CS374391A3 (sh)
DE (1) DE59108143D1 (sh)
DK (1) DK0490281T3 (sh)
ES (1) ES2093671T3 (sh)
FI (1) FI915779A (sh)
GR (1) GR3021041T3 (sh)
HU (1) HUT60505A (sh)
IE (1) IE75726B1 (sh)
IL (1) IL100298A (sh)
MX (1) MX9102468A (sh)
NO (1) NO914859L (sh)
NZ (1) NZ240900A (sh)
PL (1) PL168874B1 (sh)
PT (1) PT99747A (sh)
RO (1) RO111575B1 (sh)
RU (1) RU2073682C1 (sh)
TR (1) TR27715A (sh)
YU (1) YU187991A (sh)

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Also Published As

Publication number Publication date
CS374391A3 (en) 1992-06-17
US5659025A (en) 1997-08-19
PL168874B1 (pl) 1996-04-30
IE75726B1 (en) 1997-09-24
NO914859D0 (no) 1991-12-10
BR9105335A (pt) 1992-08-25
KR920012107A (ko) 1992-07-25
IL100298A (en) 1997-03-18
BG95575A (bg) 1993-12-24
HUT60505A (en) 1992-09-28
RU2073682C1 (ru) 1997-02-20
GR3021041T3 (en) 1996-12-31
HU913885D0 (en) 1992-02-28
IL100298A0 (en) 1992-09-06
TR27715A (tr) 1995-06-22
NO914859L (no) 1992-06-12
JP3140127B2 (ja) 2001-03-05
DE59108143D1 (de) 1996-10-10
ATE142222T1 (de) 1996-09-15
FI915779A (fi) 1992-06-12
FI915779A0 (fi) 1991-12-09
BG61484B1 (bg) 1997-09-30
CA2057475A1 (en) 1992-06-12
EP0490281A1 (de) 1992-06-17
AU8892791A (en) 1992-06-18
US5668269A (en) 1997-09-16
MX9102468A (es) 1992-06-01
NZ240900A (en) 1994-07-26
PT99747A (pt) 1992-11-30
PL292701A1 (en) 1992-09-21
US5679785A (en) 1997-10-21
DK0490281T3 (sh) 1997-02-10
AU643856B2 (en) 1993-11-25
EP0490281B1 (de) 1996-09-04
JPH04290896A (ja) 1992-10-15
IE914301A1 (en) 1992-06-17
RO111575B1 (ro) 1996-11-29
ES2093671T3 (es) 1997-01-01

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