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WO2012124941A3 - ２-하이드록시부티레이트를 모노머로 함유하고 있는 폴리하이드록시알카노에이트를 생산하는 능력을 가지는 재조합 미생물 및 이를 이용한 ２-하이드록시부티레이트를 모노머로 함유하고 있는 폴리하이드록시알카노에이트의 제조방법 - Google Patents

２-하이드록시부티레이트를 모노머로 함유하고 있는 폴리하이드록시알카노에이트를 생산하는 능력을 가지는 재조합 미생물 및 이를 이용한 ２-하이드록시부티레이트를 모노머로 함유하고 있는 폴리하이드록시알카노에이트의 제조방법 Download PDF

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Publication number
WO2012124941A3
WO2012124941A3 PCT/KR2012/001752 KR2012001752W WO2012124941A3 WO 2012124941 A3 WO2012124941 A3 WO 2012124941A3 KR 2012001752 W KR2012001752 W KR 2012001752W WO 2012124941 A3 WO2012124941 A3 WO 2012124941A3
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WO
WIPO (PCT)
Prior art keywords
hydroxybutyrate
monomer
polyhydroxyalkanoate containing
recombinant microorganism
ability
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Application number
PCT/KR2012/001752
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English (en)
French (fr)
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WO2012124941A9 (ko
WO2012124941A2 (ko
Inventor
이상엽
박시재
이승환
송봉근
이태우
Original Assignee
한국과학기술원
한국화학연구원
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Publication of WO2012124941A2 publication Critical patent/WO2012124941A2/ko
Publication of WO2012124941A3 publication Critical patent/WO2012124941A3/ko
Publication of WO2012124941A9 publication Critical patent/WO2012124941A9/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/13—Transferases (2.) transferring sulfur containing groups (2.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C12P7/625—Polyesters of hydroxy carboxylic acids

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Biological Depolymerization Polymers (AREA)
  • Polyesters Or Polycarbonates (AREA)

Abstract

본 발명은 2-하이드록시부티레이트를 모노머로 함유하고 있는 폴리하이드록시알카노에이트, 2-하이드록시부티레이트를 모노머로 함유하고 있는 폴리하이드록시알카노에이트를 생산하는 능력을 가지는 재조합 미생물 및 이를 이용한 2-하이드록시부티레이트를 모노머로 함유하고 있는 폴리하이드록시알카노에이트의 제조방법에 관한 것이다. 본 발명은 생분해성 신규 폴리머인 2-하이드록시부티레이트를 모노머로 함유하고 있는 폴리하이드록시알카노에이트 및 이를 제조할 수 있는 재조합 미생물을 제공하는 효과가 있다.
PCT/KR2012/001752 2011-03-11 2012-03-09 ２-하이드록시부티레이트를 모노머로 함유하고 있는 폴리하이드록시알카노에이트를 생산하는 능력을 가지는 재조합 미생물 및 이를 이용한 ２-하이드록시부티레이트를 모노머로 함유하고 있는 폴리하이드록시알카노에이트의 제조방법 WO2012124941A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020110022021A KR101328567B1 (ko) 2011-03-11 2011-03-11 ２­하이드록시부티레이트를 모노머로 함유하고 있는 폴리하이드록시알카노에이트를 생산하는 능력을 가지는 재조합 미생물 및 이를 이용한 ２­하이드록시부티레이트를 모노머로 함유하고 있는 폴리하이드록시알카노에이트의 제조방법
KR10-2011-0022021 2011-03-11

Publications (3)

Publication Number Publication Date
WO2012124941A2 WO2012124941A2 (ko) 2012-09-20
WO2012124941A3 true WO2012124941A3 (ko) 2012-12-27
WO2012124941A9 WO2012124941A9 (ko) 2013-02-21

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2012/001752 WO2012124941A2 (ko) 2011-03-11 2012-03-09 ２-하이드록시부티레이트를 모노머로 함유하고 있는 폴리하이드록시알카노에이트를 생산하는 능력을 가지는 재조합 미생물 및 이를 이용한 ２-하이드록시부티레이트를 모노머로 함유하고 있는 폴리하이드록시알카노에이트의 제조방법

Country Status (2)

Country Link
KR (1) KR101328567B1 (ko)
WO (1) WO2012124941A2 (ko)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101630003B1 (ko) * 2011-11-28 2016-06-13 주식회사 엘지화학 2-하이드록시알카노에이트 중합체의 제조방법
KR102088502B1 (ko) * 2015-09-03 2020-03-12 주식회사 엘지화학 4-하이드록시부티레이트, 3-하이드록시부티레이트, 2-하이드록시부티레이트 및 락테이트를 반복단위로 포함하는 사중합체 및 이의 제조방법
KR102088503B1 (ko) * 2015-09-03 2020-03-12 주식회사 엘지화학 4-하이드록시부티레이트, 3-하이드록시부티레이트 및 2-하이드록시부티레이트를 반복단위로 포함하는 삼중합체 및 이의 제조방법
KR102088504B1 (ko) * 2015-09-03 2020-03-12 주식회사 엘지화학 3-하이드록시프로피오네이트, 2-하이드록시부티레이트 및 락테이트를 반복단위로 포함하는 삼중합체 및 이의 제조방법
KR102361618B1 (ko) * 2018-01-08 2022-02-09 주식회사 엘지화학 2-하이드록시부티레이트 및 3-하이드록시부티레이트의 공중합체 및 이의 제조방법
KR102497785B1 (ko) * 2018-01-08 2023-02-07 주식회사 엘지화학 2-하이드록시부티레이트 및 락테이트의 공중합체 제조를 위한 재조합 균주 및 방법
KR20190090602A (ko) * 2018-01-25 2019-08-02 주식회사 엘지화학 2-하이드록시부티레이트, 2-하이드록시이소발레레이트 및 락테이트의 삼중합체 제조를 위한 재조합 균주 및 방법
CN115851510B (zh) * 2022-10-13 2023-09-12 深圳中科翎碳生物科技有限公司 盐单胞菌及其在联产四氢嘧啶和聚羟基脂肪酸酯的应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080275208A1 (en) * 1998-05-22 2008-11-06 Metabolix, Inc. Delaware Polyhydroxyalkanoate biopolymer compositions
KR20090078925A (ko) * 2008-01-16 2009-07-21 주식회사 엘지화학 폴리락테이트 또는 그 공중합체 생성능을 가지는 재조합미생물 및 이를 이용한 폴리락테이트 또는 그 공중합체의제조방법
KR20100090019A (ko) * 2009-02-05 2010-08-13 주식회사 엘지화학 폴리락틱산 또는 폴리락틱산 공중합체 생성능을 지닌 재조합 랄스토니아 유트로파 및 이것을 이용하여 폴리락틱산 또는 폴리락틱산 공중합체를 제조하는 방법

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080275208A1 (en) * 1998-05-22 2008-11-06 Metabolix, Inc. Delaware Polyhydroxyalkanoate biopolymer compositions
KR20090078925A (ko) * 2008-01-16 2009-07-21 주식회사 엘지화학 폴리락테이트 또는 그 공중합체 생성능을 가지는 재조합미생물 및 이를 이용한 폴리락테이트 또는 그 공중합체의제조방법
KR20100090019A (ko) * 2009-02-05 2010-08-13 주식회사 엘지화학 폴리락틱산 또는 폴리락틱산 공중합체 생성능을 지닌 재조합 랄스토니아 유트로파 및 이것을 이용하여 폴리락틱산 또는 폴리락틱산 공중합체를 제조하는 방법

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
JIA JIAN ET AL.: "Production ofpolyhydroxyalkanoates by Escherichia coli mutants with defected mixed acid fermentation pathways", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 87, no. 6, August 2010 (2010-08-01), pages 2247 - 2256 *
KEN' ICHIRO MATSUMOTO ET AL.: "Enzymatic and whole-cell synthesis of lactate-containing polyesters: toward the complete biological production of polylactate", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 85, no. 4, January 2010 (2010-01-01), pages 921 - 932 *
TAEK HO YANG ET AL.: "Biosynthesis of polylactic acid and its copolymers using evolved propionate CoA transferase and PHA synthase", BIOTECHNOLOGY AND BIOENGINEERING, vol. 105, no. 1, 1 January 2010 (2010-01-01), pages 150 - 160 *
YU KYUNG JUNG ET AL.: "Metabolic engineering of Escherichia coli for the production of polylactic acid and its copolymers", BIOTECHNOLOGY AND BIOENGINEERING, vol. 105, no. 1, 1 January 2010 (2010-01-01), pages 161 - 171 *
YUYANG SONG ET AL.: "Engineered Corynebacterium glutamicum as an endotoxin-free platform strain for lactate-based polyester production", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 93, no. 5, 30 November 2011 (2011-11-30), pages 1917 - 1925 *

Also Published As

Publication number Publication date
WO2012124941A9 (ko) 2013-02-21
WO2012124941A2 (ko) 2012-09-20
KR20120103997A (ko) 2012-09-20
KR101328567B1 (ko) 2013-11-13

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