TWI816128B - Use of extract of angelica dahurica var. formosana - Google Patents

Abstract

A use of an extract of Angelica dahuricavar. formosanais for manufacturing an agent for promoting hair growth. The extract of Angelica dahuricavar. formosanais administrated to a skin of a subject in need to promote hair growth of the subject in need. Moreover, the extract of Angelica dahuricavar. formosanais obtained by extracting a root sample of Angelica dahuricavar. formosanaat 55-65°C by water as an extractant.

Description

Uses of Duhuo Extract

The present invention relates to the use of an extract of the plant, particularly the use of the extract of the plant to prepare a preparation for promoting hair growth.

It is human nature to love beauty, and hair volume and hair color are important key factors that affect a person's appearance. However, due to factors such as abnormal diet or work and rest, excessive stress, etc., massive hair loss and baldness tend to occur at a younger age.

The hair follicle is an organ located in the dermal layer. The papillae is connected to the hair follicles with sebaceous glands to provide nutrients and oxygen to the growing hair. Therefore, if the papilla atrophies or is injured, , hair will stop growing and hair loss (also called alopecia or baldness) will occur.

Drugs commonly used to treat hair loss include minoxidil and finasteride. However, minoxidil may cause burning or irritation of the eyes and the treatment area. Itching, redness or irritation and other side effects, while finasteride may cause sexual dysfunction. In view of this, if we can further find botanical drugs that can promote hair growth, It really helps to improve the problem of hair loss.

In order to solve the above problems, the object of the present invention is to provide a use of the Indica odorifera extract, which is used to prepare preparations for promoting hair growth.

The use of the Duhuo extract of the present invention is to prepare a preparation for promoting hair growth. The Duhuo extract is applied to the skin of a desired individual to promote hair growth of the desired individual; wherein, the The Tuho extract can be obtained by extracting a Tuho sample with water at a temperature of 55 to 65°C.

Accordingly, the use of the Indica extract of the present invention is to activate hair follicle dermal papilla cells through the active ingredients extracted from the Indigo sample, so that the hair follicle dermal papilla cells have good enzyme activity (such as alkaline phosphatase activity), thereby promoting hair growth, and therefore can be used to prepare preparations for promoting hair growth, which is the effect of the present invention.

The use of the Kuo Kuo extract of the present invention, wherein the Kuo Kuo extract can be administered to the desired individual by smearing, spraying, etc. In this way, the user can easily apply the single-life extract to the skin of the desired individual.

The use of the Kusanagi extract of the present invention, wherein the Kusanagi extract can be administered to the desired individual at a dose of 1.5 to 3.0 mg/cm ² . In this way, the Duhuo extract can effectively promote hair growth.

In order to make the above and other objects, features and advantages of the present invention more obvious and understandable, the following describes the preferred embodiments of the present invention in detail with reference to the accompanying drawings:

The "preparation" mentioned in the present invention refers to an active ingredient processed and prepared into a specific dosage form, and contains a certain dose of drugs and cosmetics. The "preparation for promoting hair growth" of the present invention can activate hair follicle dermal papilla cells (dermal papilla cell (DPC cell), which enables the hair follicle dermal papilla cells to have good activity, thereby promoting hair growth. This can be understood by those with ordinary knowledge in the technical field to which the present invention belongs, and will not be described again here.

The "Independent Living Sample" mentioned in the present invention refers to the root sample of Angelica dahurica var. formosana , a plant of the Apiaceae family, which is known to have anti-inflammatory, analgesic and other effects, and also has the ability to lower blood pressure. active.

The Duhuo extract of the present invention can make hair follicle dermal papilla cells have good activity, provide sufficient nutrients and oxygen for growing hair, and prevent hair loss. Therefore, it can be used to prepare preparations that promote hair growth. The live extract can be combined with at least one pharmaceutically acceptable carrier to form the preparation, or other active ingredients that promote hair growth can be additionally added to the preparation to co-administer it to a desired individual (for example, it can be administered at 1.5 to A dose of 3.0 mg/cm ² is applied to the skin of the desired individual), the carrier system is an excipient or an additive, preferably it can jointly form a liquid, emulsion, cream or gel, etc. Various types of preparations can be conveniently used by the desired individuals.

The Tuho extract can preferably be prepared by a method comprising the following steps: providing the Tuho sample; extracting the Tuho sample using water as an extraction solvent; and concentrating a crude Tuho extract to Obtain this extract.

To be specific, the Duhuo sample can be a root sample of Angelica officinale. Workers can preferably dry the Duhuo sample in advance to obtain a dried Duhuo product with a moisture content of less than 5%; in addition, The Duhuo sample can also be pre-crushed into powder particles with a particle size of about 2 to 3 mm to increase the contact surface area between the Duhuo sample and the extraction solvent, thereby improving the extraction efficiency of subsequent extractions.

For example, 500 ml of the extraction solvent can be mixed with every 200 grams of Duhuo sample and extracted at a temperature of 55-65°C for 2 hours, so that the active ingredients rich in the Duhuo sample can be completely dissolved into the The extraction solvent is widely used by those with ordinary knowledge in the technical field to which the present invention belongs, and will not be described in detail here.

The aforementioned crude extract of Tuho can be concentrated under reduced pressure and freeze-dried to obtain the Tuho extract. Through this procedure, the active ingredients of the Tuho extract can be more concentrated, so only a small amount is needed. This alone extract can exert the best therapeutic effect.

In order to confirm that the Duhuo extract of the present invention can indeed bring good activity to hair follicle dermal papilla cells, as shown in Table 1, the Duhuo sample was extracted with water at a temperature of 60°C to obtain Group A3. Extract of Kuo Kuo extract (total yield: 8.5 grams); Extract with water at a temperature of 100°C to obtain the Kuko Kuyo extract of Group A4 (total yield: 13.6 grams); Extract at a temperature of 60°C with a concentration of 75 % of the ethanol aqueous solution to obtain the extract of Group A5 (total yield: 6.5 grams); and at a temperature of 60°C, extract the aqueous ethanol solution of 95% to obtain the extract of Group A6 (total yield: 6.5 grams). Yield is 5.6 g).

Table 1. Extraction temperatures and extraction solvents used for each group of Solo extracts Group Extraction temperature extraction solvent A3 60℃ water A4 100℃ water A5 60℃ 75% ethanol aqueous solution A6 60℃ 95% ethanol aqueous solution

Then, the Duhuo extracts from groups A3 to A6 were added to a 96-well plate cultured with hair follicle dermal papilla cells. The number of cells in each well was 1×10 ⁴ and the concentration of the Duhuo extract was 3 mg/ mL. After culturing for 24 hours in an incubator at 37°C and 5% carbon dioxide, remove the culture medium from each well and wash with Hepes BSS solution, then add 100 μL of culture medium and 20 μL of MTS assay solution, after reacting for 2.5 hours, use an enzyme immunoassay reader (ELISA reader) to detect the absorbance value at a wavelength of 490 nm, and then convert the cell growth amount of hair follicle dermal papilla cells in each group.

Please refer to Figure 1. The hair follicle dermal papilla cells that were added to the A3 group of Toko extracts had the largest cell growth, and the effect was much better than that of the A4 to A6 groups of Toko extracts (*: P ＜0.05; ** *: P <0.001). Furthermore, the cell growth-promoting effect of the Duhuo extract in Group A3 was similar to that of the positive control groups A1 and A2 (adenosine at a concentration of 10 or 5 μg/mL), and was similar to that of Group A7 that only added culture medium. There are also obvious differences.

Next, after lysing the cells in each of the aforementioned groups, centrifuge at 25°C and 13,000 rpm for 3 minutes, add the supernatant to a 96-well plate, and then add dNPP solution and alkaline phosphatase (ALP). ) solution, react at room temperature for 60 minutes, then add the stop solution, use an enzyme immunoassay reader to detect the absorbance value at a wavelength of 405 nm, and then convert the alkalinity of the hair follicle dermal papilla cells in each group Phosphatase enzyme activity.

Please refer to Figure 2. The enzyme activity of alkaline phosphatase in the dermal papilla cells of hair follicles added to the A3 group of univitae extracts is the best, and its effect is also better than that of the A4 to A6 groups of univitae extracts. It is also Better than adenosine at a concentration of 5 μg/mL (Group A2) (*: P <0.05; **: P <0.01; ***: P <0.001).

To sum up, the purpose of the Duhuo extract of the present invention is to activate the hair follicle dermal papilla cells through the active ingredients extracted from the Duhuo sample, so that the hair follicle dermal papilla cells have good enzyme activity (such as alkaline Phosphatase activity), thereby promoting hair growth, and therefore can be used to prepare preparations for promoting hair growth, which is the effect of the present invention.

Although the present invention has been disclosed using the above-mentioned preferred embodiments, they are not intended to limit the invention. Anyone skilled in the art can make various changes and modifications to the above-described embodiments without departing from the spirit and scope of the invention. The technical scope protected by the invention, therefore, the scope of protection of the invention shall be determined by the appended patent application scope.

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[Picture 1] A bar graph showing the effects of each group of Duhuo extracts on cell growth. [Picture 2] A bar graph showing the effect of each group of Duhuo extracts on the enzyme activity of alkaline phosphatase.

Claims (3)

The use of an extract of Indica solani is used to prepare a preparation that promotes hair growth. The extract of Indica solani is applied to the skin of a desired individual to promote the hair growth of the individual in need; wherein, the Indigo plant extract is used to promote hair growth of the individual. The extract is obtained by extracting a living sample with water at a temperature of 55 to 65°C. For example, the use of the lovage extract of claim 1, wherein the lovage extract is applied to the desired individual by smearing or spraying. For example, the use of the lovage extract of claim 2, wherein the lovage extract is administered to the desired individual at a dose of 1.5 to 3.0 mg/cm ² .

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