KR101654308B1 - Composition for improving hair having effect of depilation prevention and good hair - Google Patents

Abstract

The present invention provides a composition for preventing hair loss and having a hair-wool effect, which comprises a natural combination extract of a herbal medicine contained in a prescription of a ginseng root oil contained in a cultured ginseng adventitious root extract and a ginseng root extract.

Thus, the mixture of the cultured ginseng adventitious roots extract and the natural combination of herbal medicines suppressed 5α-reductase activity and exhibited excellent effects in the hair growth test using the isolated cultivation of rat rhizomal follicle (Rat vibrissa hair follicle) , Improvement and wool effect.

Hair enhancement, herbal medicine complex extract, wild ginseng root extract

Description

Technical Field [0001] The present invention relates to a composition for hair loss prevention and hair firming prevention,

The present invention relates to a composition for improving hair, and more particularly, to a hair improving composition having hair loss prevention effect and hair wool effect containing as an active ingredient a ginseng adriamycin root extract obtained by tissue culture and a herbal medicine combination extract of Dong- .

In general, hair loss is caused by male hormone, deterioration of metabolic function due to decrease of blood flow due to poor blood circulation, malnutrition, side effects due to drugs, scalp damage caused by dandruce or bacteria, genetic factors It is known. It is also known that scalp and hair are damaged due to excessive stimulation of scalp and hair due to excessive shampoo, drying, hair styling, and removal of natural moisturizing ingredients of the scalp due to chemical cleaning ingredients. Especially, in recent years, due to excessive increase of stress due to changes in the social environment, the number of people suffering from hair loss and hair damage symptoms is increasing, and age and gender limitations and boundaries are also widening.

On average, the number of human hair is about 100,000 to 120,000. Hair has a life cycle of development, growth, degeneration, and dormancy, and it is normal that the hair that enters the dormant period usually falls in the range of 50 ~ 100 per day. If the hair grows for a certain period of time, it will fall out and bring new hair. Unlike fingernails or claws, it has a cycle of "growing, resting, and falling". In the case of the molting animals, the hairs are falling at the same time because of the same hair follicle cycle (hair follicle period), so that the new hull is molted. However, since each human hair has its own mother cycle, it always maintains a constant number of hair without molting. In the case of human hair, it grows after 3 years, and after 3 months from that position, new hair is born. There are about 100,000 to 120,000 hairs, of which 70 are lost a day. More than 70 hairs that were lost three months ago are newly developed, and hair is always kept at 10 to 120 thousand.

Each hair grows and falls through anagen, catagen, and telogen with different cycles. This cycle is repeated over 3 to 6 years, resulting in an average of 50 to 100 hairs dropping normally on a daily basis.

Hair is roots in the scalp and nutrients are supplied from the blood. When the capillaries are shrunk over a long period of time and the scalp becomes thin and the hair can not grow normally, the hair of the area becomes thinner and the hair life of the specific area is short The shortened life span of the hair is not only easily lost, but also in the pores in which the hair is missing, the hair does not come out again, resulting in the appearance of a papilloma. In addition, testosterone, known to interfere with the production of energy required for hair growth and development, is transformed into DHT (Dihydrotesosterone), the activity of which is increased by the action of 5α-reductase, which is distributed around the hair follicles. To shorten the growth period of the hair and shrink the size of the hair follicle to make the hair thinner and eventually to cause hair loss.

Various anti-hair-reducing agents and wool-promoting agents have been developed for the purpose of enabling active wool to be formed in the pores of the scalp while preventing the symptoms of the bad hair caused by the hair loss phenomenon as described above.

Minoxidil-containing preparations are widely used as agents for the treatment and prevention of such alopecia. Minoxidil has been developed as a treatment for prostatitis, but nowadays it is becoming more famous as a hair-dressing agent because of wool symptoms due to side effects. However, minoxidil preparations were found to be unsuitable for long-term use due to side effects such as scalp irritation such as scalp drying, erythema, allergic contact dermatitis, facial hyperhidrosis and vasodilatation, hypotension caused by antihypertensive action, hypotension, lost. Propecia, developed by Merck, is a 5α-reductase inhibitor, a male hormone metabolizing enzyme, that prevents the conversion of testosterone, a testosterone, into dihydrotestosterone, thereby preventing hair loss. However, such effects are effective at the time of use, but when the use of the drug is stopped, hair loss is resumed as in the case of non-users after a few months, and high costs and side effects such as loss of sexual desire and erectile dysfunction occur .

Thus, minoxidil and propecia, which have been used so far, exhibit distinctive effects in preventing wool and hair loss, but are reported to be unsuitable for long-term use due to various side effects. Therefore, although most hair removal patients do not cure hair loss symptoms, they are satisfied with the temporary improvement effect by using various wool promoting agents alternately.

The present inventors have searched for the effect of wool from wool-related prescription raw materials listed in Sansam Root Extract (hereinafter referred to as "Sansam Root Extract") and Dongwoo Boo-gop (Rice ginseng root extract) for the development of raw materials having a wool effect that can be safer and longer- As a result, it was confirmed that the wool effect was excellent from the herbal medicine composite extract (hereinafter referred to as "Jinju ranun oil extract"

It is therefore an object of the present invention to provide a method for the treatment of hair loss and hair loss by using a mixture of a herbal extract, an antioxidant extract and a cultured ginseng extract, which shows little or no adverse effects such as loss of sexual desire, erectile dysfunction, hypothalamus, hypotension, To provide new wool and hair loss prevention compositions.

The present invention provides a composition for preventing hair loss and having a hair-wool effect, which comprises a natural combination extract of a herbal medicine contained in a prescription of a ginseng root oil contained in a cultured ginseng adventitious root extract and a ginseng root extract.

There is provided a cosmetic composition comprising the hair improving composition according to the present invention.

According to the present invention, since the 5α-reductase activity is inhibited and the hair growth test using the isolated cultivation of the rat rhizome hair follicle (Rat vibrissa hair follicle) shows an excellent effect, it is possible to provide hair loss prevention, improvement and wool effect.

The composition for preventing hair loss and hair for improving hair loss according to the present invention is characterized by containing a ginseng adriamycin root extract obtained by tissue culture and a natural combination extract of Chinese medicine contained in a prescription of Jinju ranchu oil contained in Dongbokgam.

Here, it is noted that Jinju ranun oil is an oriental medicine prescribed in the Dongbu Bohak section of the Dongbu bohak section, and it is said that it has an efficacy to excrete hair. The added medicinal materials include Manjang, Guri, Deokhyang, , Mangrove, clove, gangbyeong, texture, and lettuce.

Thus, the natural herbal extract of the medicinal herb composition may be an extract of Ganoderma lucidum, Guerbet, Eucalyptus, Hansikcho, Windblown, Rubus spp.

 This is a rare plant which is treated as a mysterious herb medicine together with a weekly rust oiled milk. An extract of artificially cultured wild ginseng which has been used as a therapeutic agent for various diseases and an aphrodisiac is used together.

Ginseng is an example of a plant that contains a large amount of functional ingredients. It is a ginseng that grows naturally in the mountains belonging to Araliacea and Panax ginseng CA Meyer. When the bud comes out, the flower- It belongs to a flowering plant together with a pizza plant, and it is a semicellular plant. It has been recorded as 'Shinsho' in Dongbokgop, and it has been used as a variety of disease remedy and aphrodisiac since it is a rare plant treated as a mysterious dragon.

The ginseng efficacy reported in clinical studies includes antidiabetic action, anticancer action, cardiac strengthening and blood pressure control, liver function enhancement, gastrointestinal function enhancement, stress relief and tonic effect, physical strength (tack) Anti-aging, radiation protection, anemia recovery and hematopoiesis, immunity, anti-inflammation and fragility. The efficacy of ginseng is similar to that of ginseng, but it is known to be superior in terms of pharmacological effects.

The wild ginseng is classified into four kinds such as ten kinds, geographical species, race, camphor, and more specifically, it is classified into four kinds of ginseng (natural ginseng) It is the rabbit raised by sowing this seed and the camphor (ginseng) which picked up the seed of the wild ginseng and artificially cured it in the wild state by sowing in the deep mountain. In general, wild ginseng is a very precious plant that is not easy to cultivate because it does not come out well even if seeds are sprinkled, and even if it grows, it does not pass for many years and rots away.

10 to 90% by weight of the natural herbal extract of the herbal composition and 10 to 90% by weight of the ginseng adventitious root extract of the present invention may be contained in 100% by weight of the hair improving composition according to the present invention.

Herein, the natural herbal composition extract and the cultured ginseng adriamycin root extract include water containing purified water; A lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof; Hexane; Butylene glycol; Propylene glycol; glycerin; Acetic acid; ether; And chloroform. ≪ / RTI >

Meanwhile, the composition for improving hair according to the present invention can be used as a hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair rinse, hair treatment, hair cream, hair nutrition cream, Hair cream, hair wax, hair aerosol, hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair drying agent, hair preservative, hair dye, hair wave agent, hair bleaching agent, hair gel, hair glaze, hair dresser , A hair lacquer, a hair moisturizer, a hair mousse or a hair spray.

In this case, the hair improving composition may be contained in an amount of 0.1 to 50% by weight based on the total weight of the cosmetic composition.

As described above, according to the present invention, by using a mixture of a herbal extract, a Jinju ranun oil and a tissue-cultured ginseng extract, which exhibits the effect of preventing wool and hair loss and has no side effects such as loss of sex, erectile dysfunction, hypothalamus, hypotension and tachycardia New wool and hair loss preventing compositions can be provided.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples. However, the examples and experimental examples are illustrative of the present invention, and the content of the present invention is not limited to the following examples and experimental examples.

Production Example 1 (Preparation of a green tea oil extract of this week)

The herb medicines purchased from Kyungdong market were dried and cut into 3 L of 70% aqueous ethanol solution in an amount equal to 1 kg, followed by stirring for 3 days, followed by filtration (Whatman No.5, whatman international Ltd, England). The filtrate was concentrated under reduced pressure to obtain 114 g of a caustic rancid oil-extracted powder.

Production Example 2 (Production of wild ginseng adventitia root extract)

The wild ginseng adventitious root used in the invention was obtained by tissue culture and mass propagation as described in Korean Patent No. 353636. To 1 kg of dried ginseng adventitious roots, 30 kg of 70% ethanol and 3 kg of 1,3-butylene glycol Or 3 kg of propylene glycol were mixed. Then, it was aged sufficiently and then filtered with a filter paper to obtain an extract, which was further extracted with ethanol at 50 to 60 ° C.

EXAMPLES (Preparation of compositions for preventing hair loss and improving hair with wool effect)

A hair improving composition was prepared by mixing 30% by weight of the green tea oil extract of Preparation Example 1 and 70% by weight of the extract of the wild ginseng adventitia root of Preparation Example 2.

EXPERIMENTAL EXAMPLE 1 (Hair growth effect test using separate culturing of Rat vibrissa hair follicle)

In order to examine the effect of the wool of the hair improving composition obtained in the examples, the following experiment was carried out using the organ culture method (Jindo and Tsuboi, Jpn. J. Dermatol., 107, pp769-779, J. Dermatol. 20, pp. 756-762, 1993; Philpott et al., J. Cell Sci. ≪ RTI ID = 0.0 > et al., & , 97, pp 463-471, 1990).

Vibrissa follciles isolated from 3-week-old rats were incubated in a 5% CO ₂ incubator at 37 ° C for 2 hours. 500 μl of William E medium was added to each well of a 24-well plate, and one hair follicle was added to each well, followed by incubation in a 5% CO ₂ incubator at 37 ° C. During the cultivation, the medium was changed every 3 days, and the aqueous extract solution was treated at 0.5, 1, 2.5, and 5% concentrations. The effect of the extract on the growth of the hair follicles was examined. To observe the growth of hair follicles over time, the length of hair follicles cultured with an image analyzer (Image Analyzer, DP701 controller, Olympus) was measured at day 0 and 14, and the relative follicular growth rate (%) The results are shown in Table 1 below.

[Table 1]

sample Growth rate (%) vehicle 100 Example 0.5% 99 1.0% 116 2.5% 132 5.0% 143

As a result, the maximum growth of about 43% was promoted over time, and the growth rate of the extract was dependent on the concentration. Therefore, it was confirmed that the hair follicle effect of the hair follicle by the herbal composition of the present invention is excellent.

Experimental Example 2 (5? -Reductase activity test)

[ ³ H] testosterone ([ ³ H]) added with the rat prostate 5α-reductase was used to determine whether the composition of the example was due to inhibition of 5α-reductase activity in hair follicle hair follicles ] testosterone, T) is ^[3 H] dihydro-testosterone ^([3 H] Dihydrotestosterone, the amount that is converted to DHT) were determined using the methods described in the literature (Franck et al., Bioorganic & Medicinal Chemistry, 8, pp1479 -1487,2000).

Prostate was separated from male Wistar rat 7 ~ 8 weeks of age and quenched with liquid nitrogen. The prostate was weighed and weighed 5 ml per gram of 20 mM photometric phosphate buffer saline (PBS) containing 0.32 mM sucrose (Amresco Inc, USA) and 1 mM dithiothreitol (DTT, Sigma, USA) . The prostate was pulverized, centrifuged at 1,000 rpm for 10 minutes, filtered, and the supernatant was collected and centrifuged at 100,000 g for 90 minutes using an ultracentrifuge (Beckman, USA).

To a 1.5 ml microtube was added 250 μg of 5α-reductase, 120 nCi [1,2,6,7- ³ H] -T, 200 μM NADPH (Calbiochem, US) 6.6) and reacted at 37 ° C for 30 minutes. After the reaction, 1 ml of ethyl acetate was added, centrifuged at 1,000 rpm for 10 minutes, and the supernatant was transferred to a new microtube and dried using a speed vac. This was dissolved by adding 50 μl of ethyl acetate and spotted on a thin layer chromatography (TLC, Merck, Germany) plate. The TLC chamber was developed with a developing solvent composed of 50% ethyl acetate and 50% cyclohexane. Testosterone (T) was identified by heating at 254 nm using a UV lamp (UVGL-58, Upland, USA) and by dissolving 10% sulfuric acid in dihydrotestosterone (DHT). The T and DHT sections were cut and placed into a scintillation vial and 5 ml of cocktail was added. Counter Per Minute (CPM) was measured using a liquid scintilation counter (LSC, Packard Instruments, USA). The conversion rate of testosterone (T) by 5α-reductase activity was calculated according to the above- And the relative inhibition rate of 5? -Reductase (%) are shown in Table 2 below.

[Table 2]

sample 5a-Reductase inhibition rate (%) vehicle 0 Example 0.5% 2.9 1.0% 10.3 2.5% 15.8 5.0% 26.2

As shown in Table 2, the inhibitory activity of the 5α-reductase activity was inhibited up to about 26% by increasing the concentration at all treatments. Therefore, it was confirmed that the oriental raw material composition promotes hair growth by inhibiting 5? -Reductase activity.

Formulation examples of the composition containing the extract of the present invention will be described below, but the present invention is not intended to be limited thereto but is specifically described.

Formulation Example 1 (Production of hair tonic)

The hair improving composition according to the example 10.0% Resorcinol 0.1% menthol 0.05% Panthenol 0.2% Salicylic acid 0.1% Tocopheryl acetate 0.1% Pyridoxine hydrochloride 0.1% Castor oil 5.0% Pigment Suitable amount Spices Suitable amount ethanol Suitable amount Purified water Balance system 100.0%

Formulation Example 2 (Preparation of hair conditioner)

The hair improving composition according to the example 2.5% Cetanol 3.5% Self emulsifying glycerin monostearate 1.5% Propylene glycol 2.5% Stearylmethylbenzylammonium chloride (25%) 7.0% Methyl paraoxybenzoate 0.3% Pigment Suitable amount Spices Suitable amount Purified water Balance system 100.0%

Formulation Example 3 (Preparation of hair lotion)

The hair improving composition according to the example 5.0% Resorcinol 2.0% menthol 2.0% Panthenol 0.5% Pyrrothonol amine 0.1% Spices, coloring 0.5% Purified water Balance system 100.0%

(Formulation Example 4: Production of hair soap)

The hair improving composition according to the example 0.1% Titanium dioxide 0.2% Polyethylene glycol 0.8% glycerin 0.5% Ethylenediamine tetraacetic acid 0.05% salt 1.0% Pigment Suitable amount Soap incense Suitable amount Soap base (moisture content 13%, parts by weight) Balance system 100.0%

Thus, in the case of the hair improving composition according to the embodiment of the present invention, since the 5α-reductase activity is inhibited and the hair growth test using the isolated cultivation of the rat rhizome hair follicle (Rat vibrissa hair follicle) , Improvement and wool effect.

In addition, the hair improving composition according to the present invention can be used as a hair improving agent for a hair improving composition of the present invention, which can be used as a hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair conditioner, hair treatment, Hair gel, hair gauze, hair dressing, cosmetic cream, hair wax, hair aerosol, hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair drying agent, hair preservative, hair dye, Hair lacquers, hair moisturizers, hair mousses or hair sprays.

Claims (6)

delete delete delete delete A cosmetic composition for hair, comprising a composition for preventing hair loss and having a wool effect, which comprises a natural combination extract of a medicinal herb which is contained in a prescription for a rancid oil, The tissue-cultured wild ginseng adriamycin root extract is an extract obtained by mixing 30 kg of a 70% ethanol aqueous solution and 3 kg of 1,3-butylene glycol or propylene glycol with 1 kg of wild ginseng adventitious muscle, The extracts of the natural herb complexes contained in the above mentioned Donggwoogwon prescription include extracts of Manjang, Guri, Gwangbyeol, Hwangsik, Hansikcho, Windbreak, It is an extract obtained by mixing 3 L of 70% ethanol aqueous solution with the herbal medicine contained in the rust oiled prescription, Wherein 70% to 90% by weight of the cultured ginseng adventitia root extract and 10% to 30% by weight of the natural herbal composition extract are contained in 100% by weight of the hair improving composition, Wherein the hair improving composition is contained in an amount of 1 to 10% by weight based on the total weight of the hair cosmetic composition. delete